CmaCh06G014260 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh06G014260
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionDEAD-box ATP-dependent RNA helicase
LocationCma_Chr06: 9173121 .. 9178585 (-)
RNA-Seq ExpressionCmaCh06G014260
SyntenyCmaCh06G014260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATCAGCGCCCTTCAGTTACGTCCGATATCCCGCACTTGGCGATCGACAAGTTTCTCTCTATCGTTGTCCATACATGCAGGTAGGCTTATTGATTCAGCTCATGTTACGTCGATTATGTCGATTTCAACTAGCCACATCGAATTTTCGTTTTGGTTTGCTCACTTCTAGGGTCATAAACTATTTTTGAATGATAAAGTTTAAAATGACACTTTGGATCAAAGTACAAGCGCATAATTGAAATTTGTCACATACCATTATTGAGTCGCCATTATATCTCTTTACCATTAATTTTTAATGAGAAATTAAAATTCATCTAATGCTATGCATTATCAACAAATTATAAAATATAATCGTTTTTTATAGTTAAAAAAAATAGTCATAACCAGTAAATATTTTTATAAAAAGAAAATAGCAAATAAAAAATGAAATCAATGTAACCTAATTCATTAAGTCAAAATTTATTTATTTATTTATTATTATTATTTGTGAAAAAAGAAGTCATAAAATTTTAAAATGTGATAATTTGCCCAAAATTTTACCTTATCCAATACCCTTCTGAACTTATTTGAGACGGTTGGGTTCCACAGTGCCCCGCCACTCGCAGAAGCAAGACGGCGGTTGCTTCACCACCGGCGCCGGGAACTCTCCATTACGACCCGACTCCCCATTTCTCTTACGCCCTCATCTTCTTCATCTCCTCTTTCCTCCTTAATAACCCATCCTGTATTGCGTAGGCGCGGCATTCCATTTCCAATGGCCGCAACGCTTCTTCCGTCTACCAAAATTCCTCCCCCTCGGCTGCCCGTCTCATCTGAACGCCGCCATCCAGTTTATTTACCAAAACTTATCAATAGCAATGGACCCATATGCTCCACCCCCAAATTTAGGCCTTCGAAAGCATTTATGAGCTCATCTTCTTCAAACAATGCCGTCGATTCTAATTTCGAGGCTTCCACTCTCCGCGAGATCTGTAATGGATATGTTCCCGATCATATATTGGACAGGTAAGTATCCATATTACCTCCGTATCTTGTGGGTTCCTTCTTAGTTTATCATTTCTAACAATATCTCTTCTGGGGTGTTTTCTTTTCCCTTAATTTTCTTCTCTAAAATGATGTAGAGCTGAGGAAATTGGATTTGTTGCCCCAACTGGTGTGCAGAGGCAAGCTCTTCCAGTTCTGTTTTCTGGTCAGGATTGCGTGCTTCATGCGCAGGTCAAATCTATGTATTTCAAGTAAAATTCCTGCAGGATGTGGATACTTAGTATTGTTTGTTTGTGTAAATTCTGTATATGGTTCTTTGCTTACTCTTATCCTTTTAGACAGGTTCTGGGAAGACGCTGACATATTTGCTATTGATATTTTCTGTAATCAACGCTCAGAAATCAGCTGTGCAAGCACTGATTGTAGTCCCCACCCGGGAGCTTGGAATTCAGGTAAGATTGGGCTTTGTATCTTCCACTAATTTCATTTGTACTTATCTTTCATTCTGTAGTTCTGAAGGGCGGGGTGTTTGGTTGATGCTTCTTACATGTTGATGGTAATGTTGTATTTGACCTGGTGGAATGGATGCCAGGTTACTAAAGTTGCTAGGATGTTAGCTGCAAAGCCTGCAACGGCGGATGAAGTTGGGCAGAAATCTTATGTTGTCATGGCTCTTTTAGATGGAGGCTTGTTGAGAAGACACAAGACTTGGTTGAAGGTGCTGCATTTGTCTCTTTTCTTCATGCATAGTTAATACGAAATCTCTGGTGGAGAAGTTTATTTTCATAAGTAGTAGTGTTTTGGCTAGATGGTTTGCAAAAAGTTGAATTACTTGTTGACAACATACGTTGTCTTGGCTTCTAATTGCATTGTCATCGGGTTTCATTTTGAATTTCTTCAATATGACCTTTGTAAGGGCCCAAGCCCACTGCTAGCAGATATTGTCCTCTTTGGACTTTCCCTTGAAGGGAAGGGCTTCCCCTCAAAGCTTTAAAACGCGTCTGCTAGGGAAAGGTTTCCACACCCTTATAAAGGGTGTTTCATTCTCCTCCTCAACCAATGTGGGATATTACAATCCACCCCCCTTCAGGGCCTAACGTCCTTACTAGCACACCACCTCCTATCTACCCCTTTAGGGAACAACCTCCTCGCTGGCACATCGCCGAGTGTCTAGCTCCGATACCATTTGTAACGGCCTAAGCCCACCGCTAACAGATATTGTCCTCTTTAGGCTTTCCCTTTCGGGCTTCCCCTCAAGACTTTATAACGCGTCTGCTAGAGAAAGGTTTCCACACCCTCAATAAAGGGCGTTTCGTTGTCCTCTCCCAACCAATGTGGGATGTCACAACCTTATATTGTTTTTTTCCGATTCTCTTCAATTTCTTGGCTTGTGGTTAATAGAAAAGAAGTTACATCTAGATGGTAAGTTTGAGATATTAAAATTTTCAGGCAGATCCCTCTTCTATACTGGTGGCAACAGTAGGAAGCTTGTGTCAAATGGTTGAGAGGCGATTTCTTGATCTTGCCTCACTAAAGGTGCTGGTAATTGACGAGGTACTTGCACGAAAAAGTTATTTTCAGTAAGCTTTTGGGTCATACTTTCATGCCAAATCACGTAATTGGAAGGTCGTGTAGCTATAAACTGCTGATGAATTAAGCTTGTTCGAAACGTTTATTTCAAGGAATTGACATTTTTTTCCCGCTCTTTTGCCAGGTTGATTTCATGTTTAGTTCCTCAAAGCAAGTTAGTTCTCTGAAGAAAATCTTGACATCATATTCTATGTTCAGTAATCGTCAGACTGTTTTTGCAAGTGCATCAATTCCCCAGCATAGGCATTTTCTTCAGGAATGTATACAGCAGAAGTGGACAAAGGTGTGTATATGGGTTTCTTTTTGGTTACTTCCAATATGTGATGCAAGTCAGCCATTAGTTTTATCAGCTTGTTAGAACTTTCCCTCTCTTTCTCTTGCAGAATAATGTAATCCATATTCATATTAACGCAGTTCAGCCCATGCCATCGTGTCTACTCCATAGATTTGTGGTAAGAGATTTTATTGCTCATTCAGAAGTAATTATCCTCAGCCATCTCACTGGATTTAGAATTTCTTCTTTCTAATCATTTTGAGATGTAACTTGTTTAGATATTACCTTTTTCTTGACAGATATGTGACAAGAACAAAAAACACCAAACCTTCCTGAGTGTACTACTAACAGATAAACCTCAGTCTGCAATCATTTTTGTCAGTGAGCAGGTTCGTGTTGATTGCCTTAAAGTGAGATAAATAAGTACAATATTCCAACTGCTATTGAACAACCCAAGCCTATCGCTAGCAAATATTGCCCTCTTTGGTGTTTCCGTTTCGGGTTTGTTCTCCAGGTTTTTAAAACGCATCTTTTAAAGAGAGATTTCTACACCCTTATAAGGAATTCTTCGTTCCCCTATCCAACCGATGTGGGATCTCACAATCCACCCCCCTTGGGAGCCCAGCGTCCTTGTTGGTACACCGCTCGATGTCTGACTTTGATACCATTTATAACAGTCGAAACTCACCGTAGATATTGTCCTCTTTGGGCTTTCCCTTCCAGGTTTCCCTTCAAAGTTTTTTAAGACGCATATTCTAGGGAGATGTTTCCACACCCTTATAAGGAATGCTTCGATCCCCTCTCCAACCGATGTGGGATCTCACAATCCACCCCTCTTGGGAGCCTAGCGTCCTTGTTGGTACATCGCCCGGTGTCTAACTTTAATCCATTTGTAACAGCCCAAGCCCACCGCAGCGTCCTTGTTGGTACATCGCCCGGTGTCTAACTTTAATCCATTTGTAACAGCCCAAGCCCACCGCAAATATTGTCCTCTTTGAGCTTTCCCTTATGAGTTTTCCCTCAAGGTTTTTAAAACGCATCTTCTAGGGAGTTTCCACACCCTTATAAGGAGTGCTTCGTTCCTCTCTCTAACCGATGTGGGATCTCACAATTCACCCCCCCCCCCTTGGGGGCCCAATGTCCTCGCTGGCACACCGCCCAGTATCCGACTCTGGTACCATTTGTAACAACCCAAGCCCATCGCTAGCAGATATTGTCCTCTTTGGGCTTTCCCTTCCGGGCTTCCCCTCAAGGTTTTTAAAACGCATCTTCTAGGGAGAGAGTTTCCACACCCTTATAAGAAATGCTTCGTTTCCCTCTCCAACCAATGTGGGATCTCACTGCTATTTTCGCTTGAAGCTTTCAAATTGTCATGGTTTACTATGCCATATCATATTTGCTACAAATCATCAATGAATCGAGGATCTTTTTTTCTTATGGGCTGTTTTTGTAATGTGGTTCCAAATATCATCTTCTATCGCCCTTGAAAGATTAAAATCGAGAAAACGTTTAATCTCAAATCATATTAAATTATAAGCATGTCTGTAGCTACACATCAATGCCCATTATAGGTTCATATTTTTATGTCTTGAACTCTGGATTTGAGGCAAATCTAGTTCTAGAAAGCCAATGTAATTTATTTGATTCTGTTACATTGGATTATCTTTTCTGCTCAATTTACTGAGCTCTTATTTTCAGTCAGAGAGGTCAAAGAAGGCTGGAAAGATTCCACCAGCAACCTTGATGATTGATTTCTTAAAGACTTCATATGATGGTTGTTCTGATGTCGTCCTTCTCGACGAAGACGTAAATTTCAATTCACGAGCATCTTCCCTATCGGTAAGCATAGTATGCAATCTTTTTCCAACCATTAACTGAGCGCGGTGTTAAAACTGCAGAGGATCATTTAACTGGGATGTGATATCTGATGATTCTGATCTGATATTCTAGCTAGCTGTTTGAATGAGCATGTGTTCTGAACCTTTATATCATCTTCGTTTTTCATACCGATAGAGGTTAAGCATATTCTCCTTCCTTCTCCCTTCTTTCACTCTAAAGCAATTATGATCGGAGAGAATTTACACTCACAGATTTGTAGGAAACGGTTCATTGTCGATCTTGTTTACTCTTTACATAACTATATATATATATATATCATCACTTTTCATCCATTGGGTTGGTCCAAAGCACGATATTGTATATTGAGAAAAAACGAACTACGAAAAAATATTGGTGAAGCAATATCTCAATATTTGTTGCATAAGGAGAGAAAATGGAAAAGGAGAGTGAAACCCAATAGATGAATAAGCTTCTATTCAAAATCTCAGTATGGAGAGAAAATGGAAAAGGAGAGTGAAACCCAATAGATGAATAAGCTTCTATTCAAAATCTCAGTATTTAATGTTGACTTGTAACATGTTTGTATTTGAGTTCTTGATTGTTTTGGATGCAGGAAGTGAGACAAGGAGGAAGTTATCTTATTGTAGCAACTGACTTAGTGGCCAGGGGAATCGACTTACCCGAAACGACTCATATATACAACTTTGATCTACCTCGAAGACTTAGTGGCCAGGGGAATCGACTTACCCGAAACGACTCATATATACAACTTTGA

mRNA sequence

ATGCATCAGCGCCCTTCAGTTACGTCCGATATCCCGCACTTGGCGATCGACAAGTTTCTCTCTATCGTTGTCCATACATGCAGTGCCCCGCCACTCGCAGAAGCAAGACGGCGGTTGCTTCACCACCGGCGCCGGGAACTCTCCATTACGACCCGACTCCCCATTTCTCTTACGCCCTCATCTTCTTCATCTCCTCTTTCCTCCTTAATAACCCATCCTGTATTGCGTAGGCGCGGCATTCCATTTCCAATGGCCGCAACGCTTCTTCCGTCTACCAAAATTCCTCCCCCTCGGCTGCCCGTCTCATCTGAACGCCGCCATCCAGTTTATTTACCAAAACTTATCAATAGCAATGGACCCATATGCTCCACCCCCAAATTTAGGCCTTCGAAAGCATTTATGAGCTCATCTTCTTCAAACAATGCCGTCGATTCTAATTTCGAGGCTTCCACTCTCCGCGAGATCTGTAATGGATATGTTCCCGATCATATATTGGACAGAGCTGAGGAAATTGGATTTGTTGCCCCAACTGGTGTGCAGAGGCAAGCTCTTCCAGTTCTGTTTTCTGGTCAGGATTGCGTGCTTCATGCGCAGACAGGTTCTGGGAAGACGCTGACATATTTGCTATTGATATTTTCTGTAATCAACGCTCAGAAATCAGCTGTGCAAGCACTGATTGTAGTCCCCACCCGGGAGCTTGGAATTCAGGTTACTAAAGTTGCTAGGATGTTAGCTGCAAAGCCTGCAACGGCGGATGAAGTTGGGCAGAAATCTTATGTTGTCATGGCTCTTTTAGATGGAGGCTTGTTGAGAAGACACAAGACTTGGTTGAAGGCAGATCCCTCTTCTATACTGGTGGCAACAGTAGGAAGCTTGTGTCAAATGGTTGAGAGGCGATTTCTTGATCTTGCCTCACTAAAGGTGCTGGTAATTGACGAGGTTGATTTCATGTTTAGTTCCTCAAAGCAAGTTAGTTCTCTGAAGAAAATCTTGACATCATATTCTATGTTCAGTAATCGTCAGACTGTTTTTGCAAGTGCATCAATTCCCCAGCATAGGCATTTTCTTCAGGAATGTATACAGCAGAAGTGGACAAAGAATAATGTAATCCATATTCATATTAACGCAGTTCAGCCCATGCCATCGTGTCTACTCCATAGATTTGTGATATGTGACAAGAACAAAAAACACCAAACCTTCCTGAGTGTACTACTAACAGATAAACCTCAGTCTGCAATCATTTTTGTCAGTGAGCAGTCAGAGAGGTCAAAGAAGGCTGGAAAGATTCCACCAGCAACCTTGATGATTGATTTCTTAAAGACTTCATATGATGGTTGTTCTGATGTCGTCCTTCTCGACGAAGACGTAAATTTCAATTCACGAGCATCTTCCCTATCGGAAGTGAGACAAGGAGGAAGTTATCTTATTGTAGCAACTGACTTAGTGGCCAGGGGAATCGACTTACCCGAAACGACTCATATATACAACTTTGATCTACCTCGAAGACTTAGTGGCCAGGGGAATCGACTTACCCGAAACGACTCATATATACAACTTTGA

Coding sequence (CDS)

ATGCATCAGCGCCCTTCAGTTACGTCCGATATCCCGCACTTGGCGATCGACAAGTTTCTCTCTATCGTTGTCCATACATGCAGTGCCCCGCCACTCGCAGAAGCAAGACGGCGGTTGCTTCACCACCGGCGCCGGGAACTCTCCATTACGACCCGACTCCCCATTTCTCTTACGCCCTCATCTTCTTCATCTCCTCTTTCCTCCTTAATAACCCATCCTGTATTGCGTAGGCGCGGCATTCCATTTCCAATGGCCGCAACGCTTCTTCCGTCTACCAAAATTCCTCCCCCTCGGCTGCCCGTCTCATCTGAACGCCGCCATCCAGTTTATTTACCAAAACTTATCAATAGCAATGGACCCATATGCTCCACCCCCAAATTTAGGCCTTCGAAAGCATTTATGAGCTCATCTTCTTCAAACAATGCCGTCGATTCTAATTTCGAGGCTTCCACTCTCCGCGAGATCTGTAATGGATATGTTCCCGATCATATATTGGACAGAGCTGAGGAAATTGGATTTGTTGCCCCAACTGGTGTGCAGAGGCAAGCTCTTCCAGTTCTGTTTTCTGGTCAGGATTGCGTGCTTCATGCGCAGACAGGTTCTGGGAAGACGCTGACATATTTGCTATTGATATTTTCTGTAATCAACGCTCAGAAATCAGCTGTGCAAGCACTGATTGTAGTCCCCACCCGGGAGCTTGGAATTCAGGTTACTAAAGTTGCTAGGATGTTAGCTGCAAAGCCTGCAACGGCGGATGAAGTTGGGCAGAAATCTTATGTTGTCATGGCTCTTTTAGATGGAGGCTTGTTGAGAAGACACAAGACTTGGTTGAAGGCAGATCCCTCTTCTATACTGGTGGCAACAGTAGGAAGCTTGTGTCAAATGGTTGAGAGGCGATTTCTTGATCTTGCCTCACTAAAGGTGCTGGTAATTGACGAGGTTGATTTCATGTTTAGTTCCTCAAAGCAAGTTAGTTCTCTGAAGAAAATCTTGACATCATATTCTATGTTCAGTAATCGTCAGACTGTTTTTGCAAGTGCATCAATTCCCCAGCATAGGCATTTTCTTCAGGAATGTATACAGCAGAAGTGGACAAAGAATAATGTAATCCATATTCATATTAACGCAGTTCAGCCCATGCCATCGTGTCTACTCCATAGATTTGTGATATGTGACAAGAACAAAAAACACCAAACCTTCCTGAGTGTACTACTAACAGATAAACCTCAGTCTGCAATCATTTTTGTCAGTGAGCAGTCAGAGAGGTCAAAGAAGGCTGGAAAGATTCCACCAGCAACCTTGATGATTGATTTCTTAAAGACTTCATATGATGGTTGTTCTGATGTCGTCCTTCTCGACGAAGACGTAAATTTCAATTCACGAGCATCTTCCCTATCGGAAGTGAGACAAGGAGGAAGTTATCTTATTGTAGCAACTGACTTAGTGGCCAGGGGAATCGACTTACCCGAAACGACTCATATATACAACTTTGATCTACCTCGAAGACTTAGTGGCCAGGGGAATCGACTTACCCGAAACGACTCATATATACAACTTTGA

Protein sequence

MHQRPSVTSDIPHLAIDKFLSIVVHTCSAPPLAEARRRLLHHRRRELSITTRLPISLTPSSSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPRLPVSSERRHPVYLPKLINSNGPICSTPKFRPSKAFMSSSSSNNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPTGVQRQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKVARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVERRFLDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECIQQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVSEQSERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIVATDLVARGIDLPETTHIYNFDLPRRLSGQGNRLTRNDSYIQL
Homology
BLAST of CmaCh06G014260 vs. ExPASy Swiss-Prot
Match: Q3E9C3 (DEAD-box ATP-dependent RNA helicase 58, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RH58 PE=2 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 9.6e-140
Identity = 243/378 (64.29%), Postives = 309/378 (81.75%), Query Frame = 0

Query: 135 SSSSSNNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPTGVQRQALPVLFSGQDCV 194
           SS   +N+V       TLR+IC G+VP+HIL R EEIGFV PT +QR+ALP LF+G+DC+
Sbjct: 58  SSEIESNSVTETTVPLTLRQICQGFVPEHILHRMEEIGFVFPTDIQREALPTLFTGRDCI 117

Query: 195 LHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKVARMLAAKPATADEV 254
           LHAQTGSGKTLTYLLLIFS+IN Q+S+VQA+IVVPTRELG+QVTKVARMLAAK     E+
Sbjct: 118 LHAQTGSGKTLTYLLLIFSLINPQRSSVQAVIVVPTRELGMQVTKVARMLAAK----SEI 177

Query: 255 GQKSYVVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVERRFLDLASLKVLVIDEV 314
             K   VMALLDGG LRRHK+WLKA+P +ILVATV SLC M+E+    + S++VLV+DEV
Sbjct: 178 DVKGCTVMALLDGGTLRRHKSWLKAEPPAILVATVASLCHMLEKHIFRIDSVRVLVVDEV 237

Query: 315 DFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECIQQKWTKNNVIHIHI 374
           DF+F SSKQV S++K+LTS+S    RQTVFASASIPQH+HF+ +CIQQKWTK +V+H+H+
Sbjct: 238 DFLFYSSKQVGSVRKLLTSFSSCDKRQTVFASASIPQHKHFVHDCIQQKWTKRDVVHVHV 297

Query: 375 NAVQPMPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVSEQSERSKKAGKIPPATL 434
           +A+ PMP CLLHRFV+C+K  KHQ  L++L +D P+SAIIFV EQSE+SKKAG  P  TL
Sbjct: 298 SAIMPMPLCLLHRFVMCEKTNKHQVLLALLESDAPESAIIFVGEQSEKSKKAGNDPSTTL 357

Query: 435 MIDFLKTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIVATDLVARGIDLPETTHI 494
           +++FLKTSY G  +++LL+ D+NFNSRA+SL+E+RQGG +L+V+TD+ ARGIDLPETTHI
Sbjct: 358 LMEFLKTSYKGSLEILLLEGDMNFNSRAASLTEIRQGGGFLLVSTDIAARGIDLPETTHI 417

Query: 495 YNFDLPRRLSGQGNRLTR 513
           +NFDLP+ ++   +R  R
Sbjct: 418 FNFDLPQTVTDYLHRAGR 431

BLAST of CmaCh06G014260 vs. ExPASy Swiss-Prot
Match: Q0JFN7 (DEAD-box ATP-dependent RNA helicase 58, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0970600 PE=2 SV=2)

HSP 1 Score: 453.0 bits (1164), Expect = 4.6e-126
Identity = 221/353 (62.61%), Postives = 287/353 (81.30%), Query Frame = 0

Query: 149 ASTLREICNGYVPDHILDRAEEIGFVAPTGVQRQALPVLFSGQDCVLHAQTGSGKTLTYL 208
           A+TLRE+C G VP+H+L RAEE+G+V PT VQ Q+LPVL SGQDC+LHAQTGSGKTL YL
Sbjct: 37  AATLREVCAGRVPEHVLQRAEEVGYVVPTEVQEQSLPVLLSGQDCILHAQTGSGKTLAYL 96

Query: 209 LLIFSVINAQKSAVQALIVVPTRELGIQVTKVARMLAAKPATADEVGQKSYVVMALLDGG 268
           L +FS I+  +S+VQAL+VVPTRELG+QVTKVAR+LAAK  T          VMALLDGG
Sbjct: 97  LSVFSAIDFGRSSVQALVVVPTRELGMQVTKVARILAAKACT----------VMALLDGG 156

Query: 269 LLRRHKTWLKADPSSILVATVGSLCQMVERRFLDLASLKVLVIDEVDFMFSSSKQVSSLK 328
           +LRR K+W+KA+P +I+VATV SLCQM+E+R   L S++VLVIDEVDF+F SSKQVSSL+
Sbjct: 157 MLRRQKSWVKAEPPAIIVATVASLCQMIEKRAFSLQSMRVLVIDEVDFIFGSSKQVSSLR 216

Query: 329 KILTSYSMFSNRQTVFASASIPQHRHFLQECIQQKWTKNNVIHIHINAVQPMPSCLLHRF 388
           KILTSYS  S+RQT+FASASIPQH  F+ +C+Q KWTK +V+H+H+N VQPMPS L H++
Sbjct: 217 KILTSYSAASSRQTIFASASIPQHNRFVHDCVQHKWTKTDVVHVHVNPVQPMPSHLQHKY 276

Query: 389 VICDKNKKHQTFLSVLLTDKPQSAIIFVSEQSERSKKAGKIPPATLMIDFLKTSYDGCSD 448
            IC K ++    LS+L  D P+S IIFV+EQSE+SKKAG  P  T++++FL+T+Y G  +
Sbjct: 277 AICSKKERLHVLLSLLEKDAPKSGIIFVAEQSEKSKKAGHPPSTTVVVEFLRTTYMGSLE 336

Query: 449 VVLLDEDVNFNSRASSLSEVRQGGSYLIVATDLVARGIDLPETTHIYNFDLPR 502
           V+LL+ED+NFN+RA+S +EV+ G  +L+V+TD+ +RG DLP+T+HIYNFDLP+
Sbjct: 337 VLLLEEDMNFNARATSFTEVK-GKGFLLVSTDIASRGFDLPQTSHIYNFDLPK 378

BLAST of CmaCh06G014260 vs. ExPASy Swiss-Prot
Match: P09052 (ATP-dependent RNA helicase vasa OS=Drosophila melanogaster OX=7227 GN=vas PE=1 SV=3)

HSP 1 Score: 122.5 bits (306), Expect = 1.4e-26
Identity = 95/357 (26.61%), Postives = 166/357 (46.50%), Query Frame = 0

Query: 162 DHILDRAEEIGFVAPTGVQRQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVI-----N 221
           D I+D   + G+  PT +Q+ ++PV+ SG+D +  AQTGSGKT  +LL I S +      
Sbjct: 254 DIIIDNVNKSGYKIPTPIQKCSIPVISSGRDLMACAQTGSGKTAAFLLPILSKLLEDPHE 313

Query: 222 AQKSAVQALIVVPTRELGIQVTKVARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTW 281
            +    Q +IV PTREL IQ+   AR  A           +SY+ + ++ GG   RH+  
Sbjct: 314 LELGRPQVVIVSPTRELAIQIFNEARKFAF----------ESYLKIGIVYGGTSFRHQNE 373

Query: 282 LKADPSSILVATVGSLCQMVERRFLDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYSM 341
                  +++AT G L   V+R F+     + +V+DE D M         +++I+T  +M
Sbjct: 374 CITRGCHVVIATPGRLLDFVDRTFITFEDTRFVVLDEADRMLDMGFS-EDMRRIMTHVTM 433

Query: 342 FSNRQTVFASASIPQH-RHFLQECIQQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKNK 401
               QT+  SA+ P+  +    E ++      N + + I  V    S +       +K  
Sbjct: 434 RPEHQTLMFSATFPEEIQRMAGEFLK------NYVFVAIGIVGGACSDVKQTIYEVNKYA 493

Query: 402 KHQTFLSVLLTDKPQSAIIFVSEQSERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDED 461
           K    + + L+++    I+FV    E  + A  +       +F  TS  G         D
Sbjct: 494 KRSKLIEI-LSEQADGTIVFV----ETKRGADFLASFLSEKEFPTTSIHG---------D 553

Query: 462 VNFNSRASSLSEVRQGGSYLIVATDLVARGIDLPETTHIYNFDLPRRLSGQGNRLTR 513
              + R  +L + + G   +++AT + +RG+D+    H+ N+D+P ++    +R+ R
Sbjct: 554 RLQSQREQALRDFKNGSMKVLIATSVASRGLDIKNIKHVINYDMPSKIDDYVHRIGR 579

BLAST of CmaCh06G014260 vs. ExPASy Swiss-Prot
Match: P96614 (DEAD-box ATP-dependent RNA helicase CshA OS=Bacillus subtilis (strain 168) OX=224308 GN=cshA PE=1 SV=2)

HSP 1 Score: 121.7 bits (304), Expect = 2.4e-26
Identity = 92/350 (26.29%), Postives = 170/350 (48.57%), Query Frame = 0

Query: 164 ILDRAEEIGFVAPTGVQRQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQ 223
           ++     +GF   T +Q Q +P+  S +D +  AQTG+GKT  + + +   IN +   +Q
Sbjct: 14  LMKAINRMGFEEATPIQAQTIPLGLSNKDVIGQAQTGTGKTAAFGIPLVEKINPESPNIQ 73

Query: 224 ALIVVPTRELGIQVTKVARMLAAKPATADEVGQ-KSYVVMALLDGGLLRRHKTWLKADPS 283
           A+++ PTREL IQV++             ++GQ K   V+ +  G  + R    LK +P 
Sbjct: 74  AIVIAPTRELAIQVSE----------ELYKIGQDKRAKVLPIYGGQDIGRQIRALKKNP- 133

Query: 284 SILVATVGSLCQMVERRFLDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQT 343
           +I+V T G L   + RR + L ++  +V+DE D M +    +  ++ IL++    S  QT
Sbjct: 134 NIIVGTPGRLLDHINRRTIRLNNVNTVVMDEADEMLNMG-FIDDIESILSNVP--SEHQT 193

Query: 344 VFASASIPQHRHFLQECIQQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKNKKHQTFLS 403
           +  SA++P     ++   ++  T+    H+ + A +   S +   ++   + KK  T   
Sbjct: 194 LLFSATMPAP---IKRIAERFMTEPE--HVKVKAKEMTVSNIQQFYLEVQERKKFDTLTR 253

Query: 404 VLLTDKPQSAIIFVSEQSERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRA 463
           +L    P+ AI+F        ++  ++  A  +  +      G         D+    R 
Sbjct: 254 LLDIQSPELAIVF----GRTKRRVDELAEALNLRGYAAEGIHG---------DLTQAKRM 313

Query: 464 SSLSEVRQGGSYLIVATDLVARGIDLPETTHIYNFDLPRRLSGQGNRLTR 513
            +L + ++G   ++VATD+ ARG+D+   TH+YNFD+P+      +R+ R
Sbjct: 314 VALRKFKEGAIEVLVATDVAARGLDISGVTHVYNFDVPQDPESYVHRIGR 331

BLAST of CmaCh06G014260 vs. ExPASy Swiss-Prot
Match: Q81DF9 (DEAD-box ATP-dependent RNA helicase CshE OS=Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711) OX=226900 GN=cshE PE=2 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 2.4e-26
Identity = 98/344 (28.49%), Postives = 171/344 (49.71%), Query Frame = 0

Query: 170 EIGFVAPTGVQRQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVP 229
           E G    T +Q +A+PV+ SG+D +  A+TG+GKTL ++L I   I+ + S VQALIV P
Sbjct: 22  ENGITEATPIQEKAIPVILSGKDIIGQAKTGTGKTLAFVLPILEKIDPECSDVQALIVAP 81

Query: 230 TRELGIQV-TKVARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADPSSILVAT 289
           TREL +Q+ T++ +ML         V ++   V+A+  G  + +    LK + + I+VAT
Sbjct: 82  TRELALQITTEIKKML---------VQREDINVLAIYGGQDVAQQLRKLKGN-THIVVAT 141

Query: 290 VGSLCQMVERRFLDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASAS 349
            G L   + R  +DL++L  +V+DE D M      +  ++ IL       ++QT+  SA+
Sbjct: 142 PGRLLDHIRRETIDLSNLSTIVLDEADQMLYFG-FLYDIEDILDETP--GSKQTMLFSAT 201

Query: 350 IPQHRHFLQECIQQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKNKKHQTFLSVLLTDK 409
           +P+    +++  ++   +  +I +    V      +  R +      K      V+  D+
Sbjct: 202 MPKD---IKKLAKRYMDEPQMIQVQSEEV--TVDTIEQRVIETTDRAKPDALRFVMDRDQ 261

Query: 410 PQSAIIFVSEQSERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRASSLSEV 469
           P  A+IF   +   SK          + D LK     C++   L  D+    R   +   
Sbjct: 262 PFLAVIFCRTKVRASK----------LYDNLKGLGYNCAE---LHGDIPQAKRERVMKSF 321

Query: 470 RQGGSYLIVATDLVARGIDLPETTHIYNFDLPRRLSGQGNRLTR 513
           R+     ++ATD+ ARG+D+   TH++N+D+P  +    +R+ R
Sbjct: 322 REAKIQYLIATDVAARGLDVDGVTHVFNYDIPEDVESYIHRIGR 334

BLAST of CmaCh06G014260 vs. ExPASy TrEMBL
Match: A0A6J1I860 (DEAD-box ATP-dependent RNA helicase 58, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472257 PE=4 SV=1)

HSP 1 Score: 973.4 bits (2515), Expect = 3.7e-280
Identity = 501/501 (100.00%), Postives = 501/501 (100.00%), Query Frame = 0

Query: 1   MHQRPSVTSDIPHLAIDKFLSIVVHTCSAPPLAEARRRLLHHRRRELSITTRLPISLTPS 60
           MHQRPSVTSDIPHLAIDKFLSIVVHTCSAPPLAEARRRLLHHRRRELSITTRLPISLTPS
Sbjct: 1   MHQRPSVTSDIPHLAIDKFLSIVVHTCSAPPLAEARRRLLHHRRRELSITTRLPISLTPS 60

Query: 61  SSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPRLPVSSERRHPVYLPKLINSNGP 120
           SSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPRLPVSSERRHPVYLPKLINSNGP
Sbjct: 61  SSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPRLPVSSERRHPVYLPKLINSNGP 120

Query: 121 ICSTPKFRPSKAFMSSSSSNNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPTGVQ 180
           ICSTPKFRPSKAFMSSSSSNNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPTGVQ
Sbjct: 121 ICSTPKFRPSKAFMSSSSSNNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPTGVQ 180

Query: 181 RQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV 240
           RQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV
Sbjct: 181 RQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV 240

Query: 241 ARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVERRF 300
           ARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVERRF
Sbjct: 241 ARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVERRF 300

Query: 301 LDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECI 360
           LDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECI
Sbjct: 301 LDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECI 360

Query: 361 QQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVSEQS 420
           QQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVSEQS
Sbjct: 361 QQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVSEQS 420

Query: 421 ERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIVATD 480
           ERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIVATD
Sbjct: 421 ERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIVATD 480

Query: 481 LVARGIDLPETTHIYNFDLPR 502
           LVARGIDLPETTHIYNFDLPR
Sbjct: 481 LVARGIDLPETTHIYNFDLPR 501

BLAST of CmaCh06G014260 vs. ExPASy TrEMBL
Match: A0A6J1EZV7 (DEAD-box ATP-dependent RNA helicase 58, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111441060 PE=4 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 6.6e-269
Identity = 477/501 (95.21%), Postives = 492/501 (98.20%), Query Frame = 0

Query: 1   MHQRPSVTSDIPHLAIDKFLSIVVHTCSAPPLAEARRRLLHHRRRELSITTRLPISLTPS 60
           MHQ PSV SD P++ IDKFLSI+V+TC+APPLAEARRRLLHHRRRELSITTRLPISLTPS
Sbjct: 1   MHQSPSVMSDSPYVGIDKFLSIIVYTCNAPPLAEARRRLLHHRRRELSITTRLPISLTPS 60

Query: 61  SSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPRLPVSSERRHPVYLPKLINSNGP 120
           SSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPP LPVSSERR+P+YLPKLIN+NGP
Sbjct: 61  SSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPWLPVSSERRYPIYLPKLINNNGP 120

Query: 121 ICSTPKFRPSKAFMSSSSSNNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPTGVQ 180
           ICSTPKFRP KAFMSSSS NNAVDSNFEAS+LREICNGYVPDHILDRAEEIGFVAPTGVQ
Sbjct: 121 ICSTPKFRPPKAFMSSSSLNNAVDSNFEASSLREICNGYVPDHILDRAEEIGFVAPTGVQ 180

Query: 181 RQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV 240
           RQALPVLFSG+DCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV
Sbjct: 181 RQALPVLFSGRDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV 240

Query: 241 ARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVERRF 300
           ARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADP  ILVATVGSLCQMVERRF
Sbjct: 241 ARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADPPCILVATVGSLCQMVERRF 300

Query: 301 LDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECI 360
           LDLASLKVL+IDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECI
Sbjct: 301 LDLASLKVLIIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECI 360

Query: 361 QQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVSEQS 420
           QQKWTKNN+IH+HINAVQPMPSCLLHRFV+CDKNKKHQTFLSVLLTDKPQSAIIFVSEQS
Sbjct: 361 QQKWTKNNIIHVHINAVQPMPSCLLHRFVMCDKNKKHQTFLSVLLTDKPQSAIIFVSEQS 420

Query: 421 ERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIVATD 480
           ERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRA+SLSEVRQGGSYLIVATD
Sbjct: 421 ERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRAASLSEVRQGGSYLIVATD 480

Query: 481 LVARGIDLPETTHIYNFDLPR 502
           LVARGIDLPETTHIYNFDLPR
Sbjct: 481 LVARGIDLPETTHIYNFDLPR 501

BLAST of CmaCh06G014260 vs. ExPASy TrEMBL
Match: A0A6J1C9C7 (DEAD-box ATP-dependent RNA helicase 58, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009507 PE=4 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 9.8e-196
Identity = 355/422 (84.12%), Postives = 385/422 (91.23%), Query Frame = 0

Query: 84  MAATLLPSTKIPPPRLPVSSERRHPVY----LPKLINSNGPICSTPKFRPSKAFMSSSSS 143
           MAATLLP+TK PPP L +SSER HPVY    LPK  NSNGP+CST KFRP  AF SS S 
Sbjct: 1   MAATLLPTTKSPPPWLLLSSERLHPVYFSVQLPKFNNSNGPLCSTSKFRPPLAFPSSFSL 60

Query: 144 NNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPTGVQRQALPVLFSGQDCVLHAQT 203
           NN VDSNFEASTLREIC+G+VPDHIL RAEEIGFVAPT VQRQALPVLFSG+DCVLHAQT
Sbjct: 61  NNVVDSNFEASTLREICDGHVPDHILRRAEEIGFVAPTDVQRQALPVLFSGRDCVLHAQT 120

Query: 204 GSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKVARMLAAKPATADEVGQKSY 263
           GSGKTLTYLLLIFS+INAQ+SAVQAL+VVPTRELG+QVTKVARMLAAK A A E+GQKSY
Sbjct: 121 GSGKTLTYLLLIFSIINAQRSAVQALVVVPTRELGMQVTKVARMLAAKSA-ASELGQKSY 180

Query: 264 VVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVERRFLDLASLKVLVIDEVDFMFS 323
           VVMALLDGGLLRRHK WLKADP +ILVATVGSLCQMVER+FLDL SL VLVIDEVDFMFS
Sbjct: 181 VVMALLDGGLLRRHKNWLKADPPAILVATVGSLCQMVERQFLDLGSLNVLVIDEVDFMFS 240

Query: 324 SSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECIQQKWTKNNVIHIHINAVQP 383
           SSKQVSSLKK+LTSYS FSNRQT+FASASIPQHRHFL +CIQQKWTKN+V+HIH+N +QP
Sbjct: 241 SSKQVSSLKKLLTSYSSFSNRQTIFASASIPQHRHFLHDCIQQKWTKNDVMHIHVNPMQP 300

Query: 384 MPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVSEQSERSKKAGKIPPATLMIDFL 443
           MPSCLLHRFVICDKNKKHQTF S+LLTD+PQSAIIFVSEQSE+SKKAG I P T+MIDFL
Sbjct: 301 MPSCLLHRFVICDKNKKHQTFRSLLLTDEPQSAIIFVSEQSEKSKKAGNISPTTMMIDFL 360

Query: 444 KTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIVATDLVARGIDLPETTHIYNFDL 502
           KTSY G SD++LL+ED+N+N RASSLSEVRQGG YLIVATD+VARGIDLPETTHIYNFDL
Sbjct: 361 KTSYGGYSDIILLEEDMNYNLRASSLSEVRQGGGYLIVATDIVARGIDLPETTHIYNFDL 420

BLAST of CmaCh06G014260 vs. ExPASy TrEMBL
Match: A0A1S3AX19 (DEAD-box ATP-dependent RNA helicase 58, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483729 PE=4 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 2.3e-192
Identity = 354/422 (83.89%), Postives = 376/422 (89.10%), Query Frame = 0

Query: 84  MAATLLPSTKIPPPRLPVSSERRHPVY----LPKLINSNGPICSTPKFRPSKAFMSSSSS 143
           MAAT LP+TK PPP L +SSER  PV+    LP  IN+NGPI ST KFRP +A MSSSS 
Sbjct: 1   MAATFLPTTKFPPPWLLLSSERPFPVHLSFQLPNFINTNGPIYSTSKFRPLQALMSSSSL 60

Query: 144 NNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPTGVQRQALPVLFSGQDCVLHAQT 203
            N  +SNFE STLREICNG VP+HIL R EEIGFVAPT VQRQALPVLFSG+DCVLHAQT
Sbjct: 61  KNVAESNFEGSTLREICNGRVPEHILRRTEEIGFVAPTDVQRQALPVLFSGRDCVLHAQT 120

Query: 204 GSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKVARMLAAKPATADEVGQKSY 263
           GSGKTLTYLLLIFSVINA KSAVQALIVVPTRELG+QVTKVARMLAAKPA A E G  SY
Sbjct: 121 GSGKTLTYLLLIFSVINAHKSAVQALIVVPTRELGMQVTKVARMLAAKPA-ASEDGLNSY 180

Query: 264 VVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVERRFLDLASLKVLVIDEVDFMFS 323
           VVMALLDGGLLRRHKTWLKADP  ILVATVGSLCQMVER+FLDL SL VLVIDEVDFMFS
Sbjct: 181 VVMALLDGGLLRRHKTWLKADPPEILVATVGSLCQMVERQFLDLGSLNVLVIDEVDFMFS 240

Query: 324 SSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECIQQKWTKNNVIHIHINAVQP 383
           SSKQVSSLKK+LTSYS FSNRQT+FASASIPQHRHFLQ+CIQQKWTKN VIHIHIN+ QP
Sbjct: 241 SSKQVSSLKKLLTSYSSFSNRQTIFASASIPQHRHFLQDCIQQKWTKNTVIHIHINSTQP 300

Query: 384 MPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVSEQSERSKKAGKIPPATLMIDFL 443
           MPSCLLHRFV C+KNKKHQT L++LLTD PQSAIIFVSEQSE+SKKAGKIPP TLMIDFL
Sbjct: 301 MPSCLLHRFVTCEKNKKHQTLLTLLLTDIPQSAIIFVSEQSEKSKKAGKIPPTTLMIDFL 360

Query: 444 KTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIVATDLVARGIDLPETTHIYNFDL 502
           K SY GCSDV+LL+EDVN+N RASSLSEVRQGG YLIVATD+VARGIDLPETTHIYNFDL
Sbjct: 361 KASYGGCSDVILLEEDVNYNLRASSLSEVRQGGCYLIVATDIVARGIDLPETTHIYNFDL 420

BLAST of CmaCh06G014260 vs. ExPASy TrEMBL
Match: A0A0A0L5M9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G149880 PE=4 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 3.3e-191
Identity = 350/422 (82.94%), Postives = 378/422 (89.57%), Query Frame = 0

Query: 84  MAATLLPSTKIPPPRLPVSSERRHPVY----LPKLINSNGPICSTPKFRPSKAFMSSSSS 143
           MAAT LP+TK PPP L +SSER  PV+    LP  IN+NGPI ST K RP +A +SSSS 
Sbjct: 1   MAATFLPTTKFPPPWLLLSSERSFPVHLSFQLPNFINTNGPIYSTSKSRPLQALLSSSSL 60

Query: 144 NNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPTGVQRQALPVLFSGQDCVLHAQT 203
            N  +SNFEASTLREICNG+VP+HIL R EEIGFVAPT VQRQALPVLFSG+DCVLHAQT
Sbjct: 61  KNVAESNFEASTLREICNGHVPEHILRRTEEIGFVAPTDVQRQALPVLFSGRDCVLHAQT 120

Query: 204 GSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKVARMLAAKPATADEVGQKSY 263
           GSGKTLTYLLLIFSVINA+KSAVQALIVVPTRELG+QVTKVARMLAAKPA A E G  SY
Sbjct: 121 GSGKTLTYLLLIFSVINAKKSAVQALIVVPTRELGMQVTKVARMLAAKPA-ASEDGLNSY 180

Query: 264 VVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVERRFLDLASLKVLVIDEVDFMFS 323
           VVMALLDGGLLRRHKTWLKADP  ILVATVGSLCQMVER+FLDL SL VLVIDEVDFMFS
Sbjct: 181 VVMALLDGGLLRRHKTWLKADPPEILVATVGSLCQMVERQFLDLGSLNVLVIDEVDFMFS 240

Query: 324 SSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECIQQKWTKNNVIHIHINAVQP 383
           SSKQVSSLKK+LTSYS FSNRQT+FASASIPQHRHFL +CIQQKWTK+ V+HIHIN+ QP
Sbjct: 241 SSKQVSSLKKLLTSYSSFSNRQTIFASASIPQHRHFLHDCIQQKWTKSTVVHIHINSTQP 300

Query: 384 MPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVSEQSERSKKAGKIPPATLMIDFL 443
           MPSCLLHRFV C+KNKKHQT L++LLTD PQSAIIFVSEQSE+SKKAGKIPP TLMIDFL
Sbjct: 301 MPSCLLHRFVTCEKNKKHQTLLTLLLTDIPQSAIIFVSEQSEKSKKAGKIPPTTLMIDFL 360

Query: 444 KTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIVATDLVARGIDLPETTHIYNFDL 502
           K SY GCSDV+LL+EDVN+N RASSLSE+RQGGSYLIVATD+VARGIDLPETTHIYNFDL
Sbjct: 361 KASYGGCSDVILLEEDVNYNLRASSLSELRQGGSYLIVATDIVARGIDLPETTHIYNFDL 420

BLAST of CmaCh06G014260 vs. NCBI nr
Match: XP_022973672.1 (DEAD-box ATP-dependent RNA helicase 58, chloroplastic [Cucurbita maxima])

HSP 1 Score: 973.4 bits (2515), Expect = 7.7e-280
Identity = 501/501 (100.00%), Postives = 501/501 (100.00%), Query Frame = 0

Query: 1   MHQRPSVTSDIPHLAIDKFLSIVVHTCSAPPLAEARRRLLHHRRRELSITTRLPISLTPS 60
           MHQRPSVTSDIPHLAIDKFLSIVVHTCSAPPLAEARRRLLHHRRRELSITTRLPISLTPS
Sbjct: 1   MHQRPSVTSDIPHLAIDKFLSIVVHTCSAPPLAEARRRLLHHRRRELSITTRLPISLTPS 60

Query: 61  SSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPRLPVSSERRHPVYLPKLINSNGP 120
           SSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPRLPVSSERRHPVYLPKLINSNGP
Sbjct: 61  SSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPRLPVSSERRHPVYLPKLINSNGP 120

Query: 121 ICSTPKFRPSKAFMSSSSSNNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPTGVQ 180
           ICSTPKFRPSKAFMSSSSSNNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPTGVQ
Sbjct: 121 ICSTPKFRPSKAFMSSSSSNNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPTGVQ 180

Query: 181 RQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV 240
           RQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV
Sbjct: 181 RQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV 240

Query: 241 ARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVERRF 300
           ARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVERRF
Sbjct: 241 ARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVERRF 300

Query: 301 LDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECI 360
           LDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECI
Sbjct: 301 LDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECI 360

Query: 361 QQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVSEQS 420
           QQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVSEQS
Sbjct: 361 QQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVSEQS 420

Query: 421 ERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIVATD 480
           ERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIVATD
Sbjct: 421 ERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIVATD 480

Query: 481 LVARGIDLPETTHIYNFDLPR 502
           LVARGIDLPETTHIYNFDLPR
Sbjct: 481 LVARGIDLPETTHIYNFDLPR 501

BLAST of CmaCh06G014260 vs. NCBI nr
Match: XP_022933736.1 (DEAD-box ATP-dependent RNA helicase 58, chloroplastic [Cucurbita moschata])

HSP 1 Score: 936.0 bits (2418), Expect = 1.4e-268
Identity = 477/501 (95.21%), Postives = 492/501 (98.20%), Query Frame = 0

Query: 1   MHQRPSVTSDIPHLAIDKFLSIVVHTCSAPPLAEARRRLLHHRRRELSITTRLPISLTPS 60
           MHQ PSV SD P++ IDKFLSI+V+TC+APPLAEARRRLLHHRRRELSITTRLPISLTPS
Sbjct: 1   MHQSPSVMSDSPYVGIDKFLSIIVYTCNAPPLAEARRRLLHHRRRELSITTRLPISLTPS 60

Query: 61  SSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPRLPVSSERRHPVYLPKLINSNGP 120
           SSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPP LPVSSERR+P+YLPKLIN+NGP
Sbjct: 61  SSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPWLPVSSERRYPIYLPKLINNNGP 120

Query: 121 ICSTPKFRPSKAFMSSSSSNNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPTGVQ 180
           ICSTPKFRP KAFMSSSS NNAVDSNFEAS+LREICNGYVPDHILDRAEEIGFVAPTGVQ
Sbjct: 121 ICSTPKFRPPKAFMSSSSLNNAVDSNFEASSLREICNGYVPDHILDRAEEIGFVAPTGVQ 180

Query: 181 RQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV 240
           RQALPVLFSG+DCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV
Sbjct: 181 RQALPVLFSGRDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV 240

Query: 241 ARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVERRF 300
           ARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADP  ILVATVGSLCQMVERRF
Sbjct: 241 ARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADPPCILVATVGSLCQMVERRF 300

Query: 301 LDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECI 360
           LDLASLKVL+IDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECI
Sbjct: 301 LDLASLKVLIIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECI 360

Query: 361 QQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVSEQS 420
           QQKWTKNN+IH+HINAVQPMPSCLLHRFV+CDKNKKHQTFLSVLLTDKPQSAIIFVSEQS
Sbjct: 361 QQKWTKNNIIHVHINAVQPMPSCLLHRFVMCDKNKKHQTFLSVLLTDKPQSAIIFVSEQS 420

Query: 421 ERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIVATD 480
           ERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRA+SLSEVRQGGSYLIVATD
Sbjct: 421 ERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRAASLSEVRQGGSYLIVATD 480

Query: 481 LVARGIDLPETTHIYNFDLPR 502
           LVARGIDLPETTHIYNFDLPR
Sbjct: 481 LVARGIDLPETTHIYNFDLPR 501

BLAST of CmaCh06G014260 vs. NCBI nr
Match: XP_023539234.1 (DEAD-box ATP-dependent RNA helicase 58, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 931.4 bits (2406), Expect = 3.4e-267
Identity = 480/504 (95.24%), Postives = 491/504 (97.42%), Query Frame = 0

Query: 1   MHQRPSVTSDIPHLAIDKFLSIVVHTCSAPPLAEARRRLLHHRRRELSITTRLPISLTP- 60
           MHQ PSV SD P+L IDKFLS +V+TCSAPPLAEARRRLLHHRRR+LSITTRLPISLTP 
Sbjct: 18  MHQSPSVMSDSPYLGIDKFLSSIVYTCSAPPLAEARRRLLHHRRRKLSITTRLPISLTPS 77

Query: 61  --SSSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPRLPVSSERRHPVYLPKLINS 120
             SSSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPP LPVSSERR+P+YLP LINS
Sbjct: 78  SSSSSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPWLPVSSERRYPIYLPNLINS 137

Query: 121 NGPICSTPKFRPSKAFMSSSSSNNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPT 180
           NGPICSTPKFRP KAFMSSSS NNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPT
Sbjct: 138 NGPICSTPKFRPPKAFMSSSSLNNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPT 197

Query: 181 GVQRQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQV 240
           GVQRQALPVLFSG+DCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQV
Sbjct: 198 GVQRQALPVLFSGRDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQV 257

Query: 241 TKVARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVE 300
           TKVARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADP SILVATVGSLCQMVE
Sbjct: 258 TKVARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADPPSILVATVGSLCQMVE 317

Query: 301 RRFLDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQ 360
           RRFLDLASL+VLVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQ
Sbjct: 318 RRFLDLASLRVLVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQ 377

Query: 361 ECIQQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVS 420
           ECIQQKWTKNNVIH+HINAVQPMPSCLLHRFV+CDKNKKHQTFLSVLLTDKPQSAIIFVS
Sbjct: 378 ECIQQKWTKNNVIHVHINAVQPMPSCLLHRFVMCDKNKKHQTFLSVLLTDKPQSAIIFVS 437

Query: 421 EQSERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIV 480
           EQSERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRA+SLSEVRQGGSYLIV
Sbjct: 438 EQSERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRAASLSEVRQGGSYLIV 497

Query: 481 ATDLVARGIDLPETTHIYNFDLPR 502
           ATDLVARGIDLPETTHIYNFDLPR
Sbjct: 498 ATDLVARGIDLPETTHIYNFDLPR 521

BLAST of CmaCh06G014260 vs. NCBI nr
Match: KAG6597460.1 (DEAD-box ATP-dependent RNA helicase 58, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 921.0 bits (2379), Expect = 4.6e-264
Identity = 472/501 (94.21%), Postives = 488/501 (97.41%), Query Frame = 0

Query: 1   MHQRPSVTSDIPHLAIDKFLSIVVHTCSAPPLAEARRRLLHHRRRELSITTRLPISLTPS 60
           +HQ PSV SD P++ IDKFLS +V+TC+APPLAEARRRLLHHRRRELSITTRLPI LTP 
Sbjct: 356 LHQSPSVMSDSPYVGIDKFLSSIVYTCNAPPLAEARRRLLHHRRRELSITTRLPIFLTP- 415

Query: 61  SSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPRLPVSSERRHPVYLPKLINSNGP 120
            SSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPP LPVSSERR+P+YLPKLINSNGP
Sbjct: 416 -SSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPWLPVSSERRYPIYLPKLINSNGP 475

Query: 121 ICSTPKFRPSKAFMSSSSSNNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPTGVQ 180
           ICSTPKFRP KAFMSSSS NNAVDSNFEAS+LREICNGYVPDHILDRAEEIGFVAPTGVQ
Sbjct: 476 ICSTPKFRPLKAFMSSSSLNNAVDSNFEASSLREICNGYVPDHILDRAEEIGFVAPTGVQ 535

Query: 181 RQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV 240
           RQALPVLFSG+DCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV
Sbjct: 536 RQALPVLFSGRDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV 595

Query: 241 ARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVERRF 300
           ARMLAAKPATADEVG+KSYVVMALLDGGLLRRHKTWLKADP SILVATVGSLCQMVERRF
Sbjct: 596 ARMLAAKPATADEVGEKSYVVMALLDGGLLRRHKTWLKADPPSILVATVGSLCQMVERRF 655

Query: 301 LDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECI 360
           LDLASLKVL+IDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECI
Sbjct: 656 LDLASLKVLIIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECI 715

Query: 361 QQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVSEQS 420
           QQKWTKNNVIH+HINAVQPMPSCLLHRFV+CDKNKKHQTFL VLLTDKPQSAIIFVSEQS
Sbjct: 716 QQKWTKNNVIHVHINAVQPMPSCLLHRFVMCDKNKKHQTFLRVLLTDKPQSAIIFVSEQS 775

Query: 421 ERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIVATD 480
           ERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRA+SLSEVRQGGSYLIVATD
Sbjct: 776 ERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRAASLSEVRQGGSYLIVATD 835

Query: 481 LVARGIDLPETTHIYNFDLPR 502
           LVARGIDLPETTHIYNFDLP+
Sbjct: 836 LVARGIDLPETTHIYNFDLPQ 854

BLAST of CmaCh06G014260 vs. NCBI nr
Match: KAG7028919.1 (DEAD-box ATP-dependent RNA helicase 58, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 895.2 bits (2312), Expect = 2.7e-256
Identity = 473/553 (85.53%), Postives = 487/553 (88.07%), Query Frame = 0

Query: 1   MHQRPSVTSDIPHLAIDKFLSIVVHTCSAPPLAEARRRLLHHRRRELSITTRLPISLTPS 60
           +HQ PSV SD P++ IDKFLS +V+TC+APPLAEARRRLLHHRRRELSITTRLPI LTP 
Sbjct: 375 LHQSPSVMSDSPYVGIDKFLSSIVYTCNAPPLAEARRRLLHHRRRELSITTRLPIFLTP- 434

Query: 61  SSSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPRLPVSSERRHPVYLPKLINSNGP 120
            SSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPP LPVSSERR+ +YLPKLINSNGP
Sbjct: 435 -SSSPLSSLITHPVLRRRGIPFPMAATLLPSTKIPPPWLPVSSERRYSIYLPKLINSNGP 494

Query: 121 ICSTPKFRPSKAFMSSSSSNNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPTGVQ 180
           ICSTPKFRP KAFMSSSS NNAVDSNFEAS+LREICNGYVPDHILDRAEEIGFVAPTGVQ
Sbjct: 495 ICSTPKFRPLKAFMSSSSLNNAVDSNFEASSLREICNGYVPDHILDRAEEIGFVAPTGVQ 554

Query: 181 RQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV 240
           RQALPVLFSG+DCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV
Sbjct: 555 RQALPVLFSGRDCVLHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKV 614

Query: 241 ARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLK---------------------- 300
           ARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLK                      
Sbjct: 615 ARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKGPTSSLTLVPFYDRCGTLTKST 674

Query: 301 ------------------------------ADPSSILVATVGSLCQMVERRFLDLASLKV 360
                                         ADP  ILVATVGSLCQMVERRFLDLASLKV
Sbjct: 675 SLQGLTSLTGTPSRIYPPSGNNLLAGTSLDADPPCILVATVGSLCQMVERRFLDLASLKV 734

Query: 361 LVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECIQQKWTKNN 420
           LVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECIQQKWTKNN
Sbjct: 735 LVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECIQQKWTKNN 794

Query: 421 VIHIHINAVQPMPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVSEQSERSKKAGK 480
           VIH+HINAVQPMPSCLLHRFV+CDKNKKHQTFLSVLLTDKP+SAIIFVSEQSERSKKAGK
Sbjct: 795 VIHVHINAVQPMPSCLLHRFVMCDKNKKHQTFLSVLLTDKPRSAIIFVSEQSERSKKAGK 854

Query: 481 IPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIVATDLVARGIDL 502
           IPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRA+SLSEVRQGGSYLIVATDLVARGIDL
Sbjct: 855 IPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRAASLSEVRQGGSYLIVATDLVARGIDL 914

BLAST of CmaCh06G014260 vs. TAIR 10
Match: AT5G19210.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 498.4 bits (1282), Expect = 6.8e-141
Identity = 243/378 (64.29%), Postives = 309/378 (81.75%), Query Frame = 0

Query: 135 SSSSSNNAVDSNFEASTLREICNGYVPDHILDRAEEIGFVAPTGVQRQALPVLFSGQDCV 194
           SS   +N+V       TLR+IC G+VP+HIL R EEIGFV PT +QR+ALP LF+G+DC+
Sbjct: 58  SSEIESNSVTETTVPLTLRQICQGFVPEHILHRMEEIGFVFPTDIQREALPTLFTGRDCI 117

Query: 195 LHAQTGSGKTLTYLLLIFSVINAQKSAVQALIVVPTRELGIQVTKVARMLAAKPATADEV 254
           LHAQTGSGKTLTYLLLIFS+IN Q+S+VQA+IVVPTRELG+QVTKVARMLAAK     E+
Sbjct: 118 LHAQTGSGKTLTYLLLIFSLINPQRSSVQAVIVVPTRELGMQVTKVARMLAAK----SEI 177

Query: 255 GQKSYVVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVERRFLDLASLKVLVIDEV 314
             K   VMALLDGG LRRHK+WLKA+P +ILVATV SLC M+E+    + S++VLV+DEV
Sbjct: 178 DVKGCTVMALLDGGTLRRHKSWLKAEPPAILVATVASLCHMLEKHIFRIDSVRVLVVDEV 237

Query: 315 DFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRHFLQECIQQKWTKNNVIHIHI 374
           DF+F SSKQV S++K+LTS+S    RQTVFASASIPQH+HF+ +CIQQKWTK +V+H+H+
Sbjct: 238 DFLFYSSKQVGSVRKLLTSFSSCDKRQTVFASASIPQHKHFVHDCIQQKWTKRDVVHVHV 297

Query: 375 NAVQPMPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAIIFVSEQSERSKKAGKIPPATL 434
           +A+ PMP CLLHRFV+C+K  KHQ  L++L +D P+SAIIFV EQSE+SKKAG  P  TL
Sbjct: 298 SAIMPMPLCLLHRFVMCEKTNKHQVLLALLESDAPESAIIFVGEQSEKSKKAGNDPSTTL 357

Query: 435 MIDFLKTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSYLIVATDLVARGIDLPETTHI 494
           +++FLKTSY G  +++LL+ D+NFNSRA+SL+E+RQGG +L+V+TD+ ARGIDLPETTHI
Sbjct: 358 LMEFLKTSYKGSLEILLLEGDMNFNSRAASLTEIRQGGGFLLVSTDIAARGIDLPETTHI 417

Query: 495 YNFDLPRRLSGQGNRLTR 513
           +NFDLP+ ++   +R  R
Sbjct: 418 FNFDLPQTVTDYLHRAGR 431

BLAST of CmaCh06G014260 vs. TAIR 10
Match: AT5G19210.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 362.5 bits (929), Expect = 5.8e-100
Identity = 174/278 (62.59%), Postives = 226/278 (81.29%), Query Frame = 0

Query: 235 IQVTKVARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQ 294
           +QVTKVARMLAAK     E+  K   VMALLDGG LRRHK+WLKA+P +ILVATV SLC 
Sbjct: 1   MQVTKVARMLAAK----SEIDVKGCTVMALLDGGTLRRHKSWLKAEPPAILVATVASLCH 60

Query: 295 MVERRFLDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYSMFSNRQTVFASASIPQHRH 354
           M+E+    + S++VLV+DEVDF+F SSKQV S++K+LTS+S    RQTVFASASIPQH+H
Sbjct: 61  MLEKHIFRIDSVRVLVVDEVDFLFYSSKQVGSVRKLLTSFSSCDKRQTVFASASIPQHKH 120

Query: 355 FLQECIQQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKNKKHQTFLSVLLTDKPQSAII 414
           F+ +CIQQKWTK +V+H+H++A+ PMP CLLHRFV+C+K  KHQ  L++L +D P+SAII
Sbjct: 121 FVHDCIQQKWTKRDVVHVHVSAIMPMPLCLLHRFVMCEKTNKHQVLLALLESDAPESAII 180

Query: 415 FVSEQSERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDEDVNFNSRASSLSEVRQGGSY 474
           FV EQSE+SKKAG  P  TL+++FLKTSY G  +++LL+ D+NFNSRA+SL+E+RQGG +
Sbjct: 181 FVGEQSEKSKKAGNDPSTTLLMEFLKTSYKGSLEILLLEGDMNFNSRAASLTEIRQGGGF 240

Query: 475 LIVATDLVARGIDLPETTHIYNFDLPRRLSGQGNRLTR 513
           L+V+TD+ ARGIDLPETTHI+NFDLP+ ++   +R  R
Sbjct: 241 LLVSTDIAARGIDLPETTHIFNFDLPQTVTDYLHRAGR 274

BLAST of CmaCh06G014260 vs. TAIR 10
Match: AT1G55150.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 107.8 bits (268), Expect = 2.6e-23
Identity = 97/372 (26.08%), Postives = 167/372 (44.89%), Query Frame = 0

Query: 161 PDHILDRAEEIGFVAPTGVQRQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVINAQKS 220
           PD++L+  ++ GF  PT +Q Q  P+   G+D +  A+TGSGKTL+YLL     +NAQ  
Sbjct: 107 PDYVLEEVKKAGFTEPTPIQSQGWPMAMKGRDLIGIAETGSGKTLSYLLPAIVHVNAQPM 166

Query: 221 AVQ-----ALIVVPTRELGIQVTKVARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKT 280
                    L++ PTREL +Q+ +           A + G  S +    + GG+ +  + 
Sbjct: 167 LAHGDGPIVLVLAPTRELAVQIQQ----------EASKFGSSSKIKTTCIYGGVPKGPQV 226

Query: 281 WLKADPSSILVATVGSLCQMVERRFLDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYS 340
                   I++AT G L  M+E    +L  +  LV+DE D M         ++KI++   
Sbjct: 227 RDLQKGVEIVIATPGRLIDMMESNNTNLRRVTYLVLDEADRMLDMGFD-PQIRKIVS--H 286

Query: 341 MFSNRQTVFASASIPQHRHFLQECIQQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKNK 400
           +  +RQT++ SA+ P+      E + +K+  N                  ++ +I   + 
Sbjct: 287 IRPDRQTLYWSATWPKE----VEQLSKKFLYNP-----------------YKVIIGSSDL 346

Query: 401 KHQTFLSVLLTDKPQSAIIFVSEQSERSKKAGK----IPPATLMIDFLKTSYDGCSDV-- 460
           K          ++    I+ V  +S++  K  K    I   + ++ FL T   GC  +  
Sbjct: 347 K---------ANRAIRQIVDVISESQKYNKLVKLLEDIMDGSRILVFLDTK-KGCDQITR 406

Query: 461 ---------VLLDEDVNFNSRASSLSEVRQGGSYLIVATDLVARGIDLPETTHIYNFDLP 513
                    + +  D +   R   LSE R G S ++ ATD+ ARG+D+ +  ++ N+D P
Sbjct: 407 QLRMDGWPALSIHGDKSQAERDWVLSEFRSGKSPIMTATDVAARGLDVKDVKYVINYDFP 434

BLAST of CmaCh06G014260 vs. TAIR 10
Match: AT1G12770.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 7.5e-23
Identity = 103/409 (25.18%), Postives = 166/409 (40.59%), Query Frame = 0

Query: 147 FEASTLREICNGYVPDHILDRAEEIGFVAPTGVQRQALPVLFSGQDCVLHAQTGSGKTLT 206
           F A +  E+    +PD +LD  E  GF  PT VQ  A+P +  G D V+ + TGSGKTL 
Sbjct: 107 FSAKSFEEL---GLPDSLLDSLEREGFSVPTDVQSAAVPAIIKGHDAVIQSYTGSGKTLA 166

Query: 207 YLLLIFSVI-------------NAQKSAVQALIVVPTRELGIQVTKVARMLAAKPATADE 266
           YLL I S I             N +++ +QA+IV P+RELG+Q+ +    L         
Sbjct: 167 YLLPILSEIGPLAEKSRSSHSENDKRTEIQAMIVAPSRELGMQIVREVEKLLGP------ 226

Query: 267 VGQKSYVVMALLDGGLLRRHKTWLKADPSSILVATVGSLCQMVERRFLDLASLKVLVIDE 326
                 +V  L+ G    R +  LK +  +I+V T G + ++ +   L     + LV+DE
Sbjct: 227 --VHRRMVQQLVGGANRMRQEEALKKNKPAIVVGTPGRIAEISKGGKLHTHGCRFLVLDE 286

Query: 327 VDFMFSSSKQVSSLKKILTSYSMFS------------NRQTVFASASIPQHRHFLQECIQ 386
           VD + S + +   + +IL      S            NRQT+  SA++P    F      
Sbjct: 287 VDELLSFNFR-EDIHRILEHVGKRSGAGPKGEVDERANRQTILVSATVP----FSVIRAA 346

Query: 387 QKWTKNNVIHIHINAVQP------------------------------MPSCLLHRFVIC 446
           + W+   V+ +  N V P                              +P  L H + I 
Sbjct: 347 KSWSHEPVL-VQANKVTPLDTVQPSAPVMSLTPTTSEADGQIQTTIQSLPPALKHYYCIS 406

Query: 447 DKNKKHQTFLSVLLTDKPQSAIIFVSEQSERSKKAGKIPPATLMIDFLKTSYDGCSDVVL 501
               K  T    +     QS I F++   +      K+    +             +   
Sbjct: 407 KHQHKVDTLRRCVHALDAQSVIAFMNHSRQLKDVVYKLEARGM-------------NSAE 466

BLAST of CmaCh06G014260 vs. TAIR 10
Match: AT3G06480.1 (DEAD box RNA helicase family protein )

HSP 1 Score: 105.5 bits (262), Expect = 1.3e-22
Identity = 91/358 (25.42%), Postives = 156/358 (43.58%), Query Frame = 0

Query: 160 VPDHILDRAEEIGFVAPTGVQRQALPVLFSGQDCVLHAQTGSGKTLTYLLLIFSVI---- 219
           +P  IL      GF +PT +Q Q  P+    +D V  A+TGSGKTL YL+  F ++    
Sbjct: 442 LPPEILRELLSAGFPSPTPIQAQTWPIALQSRDIVAIAKTGSGKTLGYLIPAFILLRHCR 501

Query: 220 NAQKSAVQALIVVPTRELGIQVTKVARMLAAKPATADEVGQKSYVVMALLDGGLLRRHKT 279
           N  ++    LI+ PTREL  Q+   A             G+ S +    L GG  +  + 
Sbjct: 502 NDSRNGPTVLILAPTRELATQIQDEAL----------RFGRSSRISCTCLYGGAPKGPQL 561

Query: 280 WLKADPSSILVATVGSLCQMVERRFLDLASLKVLVIDEVDFMFSSSKQVSSLKKILTSYS 339
                 + I+VAT G L  ++E + +D   + +LV+DE D M     +   ++KI+    
Sbjct: 562 KELERGADIVVATPGRLNDILEMKMIDFQQVSLLVLDEADRMLDMGFE-PQIRKIVNEIP 621

Query: 340 MFSNRQTVFASASIPQH-RHFLQECIQQKWTKNNVIHIHINAVQPMPSCLLHRFVICDKN 399
               RQT+  +A+ P+  R    + +       N + ++I  V  + +           N
Sbjct: 622 --PRRQTLMYTATWPKEVRKIASDLLV------NPVQVNIGRVDELAA-----------N 681

Query: 400 KKHQTFLSVLLTDKPQSAIIFVSEQSERSKKAGKIPPATLMIDFLKTSYDGCSDVVLLDE 459
           K    ++ V+   + +  +  +    ER  K         + D L  S       V++  
Sbjct: 682 KAITQYVEVVPQMEKERRLEQILRSQERGSKVIIFCSTKRLCDHLARSVGRHFGAVVIHG 741

Query: 460 DVNFNSRASSLSEVRQGGSYLIVATDLVARGIDLPETTHIYNFDLPRRLSGQGNRLTR 513
           D     R   L++ R G S +++ATD+ ARG+D+ +   + N+D P  +    +R+ R
Sbjct: 742 DKTQGERDWVLNQFRSGKSCVLIATDVAARGLDIKDIRVVINYDFPTGVEDYVHRIGR 769

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3E9C39.6e-14064.29DEAD-box ATP-dependent RNA helicase 58, chloroplastic OS=Arabidopsis thaliana OX... [more]
Q0JFN74.6e-12662.61DEAD-box ATP-dependent RNA helicase 58, chloroplastic OS=Oryza sativa subsp. jap... [more]
P090521.4e-2626.61ATP-dependent RNA helicase vasa OS=Drosophila melanogaster OX=7227 GN=vas PE=1 S... [more]
P966142.4e-2626.29DEAD-box ATP-dependent RNA helicase CshA OS=Bacillus subtilis (strain 168) OX=22... [more]
Q81DF92.4e-2628.49DEAD-box ATP-dependent RNA helicase CshE OS=Bacillus cereus (strain ATCC 14579 /... [more]
Match NameE-valueIdentityDescription
A0A6J1I8603.7e-280100.00DEAD-box ATP-dependent RNA helicase 58, chloroplastic OS=Cucurbita maxima OX=366... [more]
A0A6J1EZV76.6e-26995.21DEAD-box ATP-dependent RNA helicase 58, chloroplastic OS=Cucurbita moschata OX=3... [more]
A0A6J1C9C79.8e-19684.12DEAD-box ATP-dependent RNA helicase 58, chloroplastic isoform X1 OS=Momordica ch... [more]
A0A1S3AX192.3e-19283.89DEAD-box ATP-dependent RNA helicase 58, chloroplastic isoform X1 OS=Cucumis melo... [more]
A0A0A0L5M93.3e-19182.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G149880 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022973672.17.7e-280100.00DEAD-box ATP-dependent RNA helicase 58, chloroplastic [Cucurbita maxima][more]
XP_022933736.11.4e-26895.21DEAD-box ATP-dependent RNA helicase 58, chloroplastic [Cucurbita moschata][more]
XP_023539234.13.4e-26795.24DEAD-box ATP-dependent RNA helicase 58, chloroplastic [Cucurbita pepo subsp. pep... [more]
KAG6597460.14.6e-26494.21DEAD-box ATP-dependent RNA helicase 58, chloroplastic, partial [Cucurbita argyro... [more]
KAG7028919.12.7e-25685.53DEAD-box ATP-dependent RNA helicase 58, chloroplastic [Cucurbita argyrosperma su... [more]
Match NameE-valueIdentityDescription
AT5G19210.26.8e-14164.29P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G19210.15.8e-10062.59P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G55150.12.6e-2326.08DEA(D/H)-box RNA helicase family protein [more]
AT1G12770.17.5e-2325.18P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G06480.11.3e-2225.42DEAD box RNA helicase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 172..388
e-value: 3.6E-34
score: 129.5
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 184..367
score: 19.405754
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 178..351
e-value: 7.0E-28
score: 97.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 124..370
e-value: 4.4E-46
score: 159.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 382..519
e-value: 9.4E-15
score: 56.2
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 160..353
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 304..503
IPR001650Helicase, C-terminalPFAMPF00271Helicase_Ccoord: 398..518
e-value: 1.9E-10
score: 41.1
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 396..519
score: 10.025928
NoneNo IPR availablePANTHERPTHR47958ATP-DEPENDENT RNA HELICASE DBP3coord: 129..501
NoneNo IPR availablePANTHERPTHR47958:SF95DEAD-BOX ATP-DEPENDENT RNA HELICASE 58, CHLOROPLASTICcoord: 129..501
NoneNo IPR availableCDDcd00268DEADccoord: 164..373
e-value: 1.43468E-51
score: 172.241

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G014260.1CmaCh06G014260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0005681 spliceosomal complex
molecular_function GO:0005524 ATP binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0003724 RNA helicase activity
molecular_function GO:0003676 nucleic acid binding