CmaCh06G008570 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh06G008570 vs. ExPASy Swiss-Prot
Match: P30181 (DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 4.0e-295
Identity = 575/939 (61.24%), Postives = 678/939 (72.20%), Query Frame = 0

Query: 1   MAVETFGKAPTFDE---DDEDNMPIVFKRSS--ASRQNQLDSEAKQR----LNKLSGRQG 60
           M  ET  K P  D    D +D+ P+ FKR++  AS  NQ  S +++       K+S  + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDVCSANGQTSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTD 120
           S V S NG        TT   KTS +KSS        +SS +AS  KSP      S   D
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSSM------PSSSSKASPAKSPLRNDMPSTVKD 120

Query: 121 SQSRHLVKAAVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNK----- 180
                  ++  K E++        DSEDD+PLS  L G   P     V +    K     
Sbjct: 121 RSQLQKDQSECKIEHE--------DSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 --VEKSSA----DSDDEIPLSSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDK 240
             ++++S     +SDDE P+SS F  K  S +SGGNQ   DEKKPL+ K+HQNG    ++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPGVISSKRPLPNADSHLPQFSSKKPKLSDSSTPL--------------GSKLVS--V 300
           +   G +  KRPL   +S   Q S KK K+S S T +              G  LVS  +
Sbjct: 241 VPN-GKVLGKRPL-EKNSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPKM 300

Query: 301 KAKQ-------EVDDNDDHIPISQRMKKVAVSANKSLSSKKTVS-------------KVV 360
           KAKQ         DD+DD +PIS+R K  + ++N S +  K V               VV
Sbjct: 301 KAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNVV 360

Query: 361 SSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVKMIY 420
           S   +   K +KK  K SKY  S+K  PSSGDGQKKWT+LVHNGVIFPPPY+ HG+K++Y
Sbjct: 361 SPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKILY 420

Query: 421 KGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDDCDFTP 480
           KGK VDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDCDFTP
Sbjct: 421 KGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFTP 480

Query: 481 IYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLFRGR 540
           IY+W+  EKEKKKQM+TEEKKALKEEKMKQEEKYMWA++DGVKEK+GNFRVEPPGLFRGR
Sbjct: 481 IYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRGR 540

Query: 541 GEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINPK 600
           GEHPKMGKLK+RIHP +IT+NIGK APIPECPI GERWKEV+HDNTVTWLAFW DPINPK
Sbjct: 541 GEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINPK 600

Query: 601 EFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLI 660
           EFKYVFL A SSLKG SDKEKYEKAR L D+I NIR  YTK+FT+KD   RQIAVATYLI
Sbjct: 601 EFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYLI 660

Query: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
           DKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+
Sbjct: 661 DKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVY 720

Query: 721 KAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
           KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKD
Sbjct: 721 KAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKD 780

Query: 781 GDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAK 840
           GDV +KIV+YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LDRAK
Sbjct: 781 GDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAK 840

Query: 841 KGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKINYLD 884
           KGKPPL+ +DGK+ R+L P   EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLD
Sbjct: 841 KGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLD 900

BLAST of CmaCh06G008570 vs. ExPASy Swiss-Prot
Match: Q9FJ79 (DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1)

HSP 1 Score: 1013.8 bits (2620), Expect = 1.2e-294
Identity = 571/940 (60.74%), Postives = 687/940 (73.09%), Query Frame = 0

Query: 1   MAVETFGKAPT------FDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDV 60
           MA E F K         ++++DED++P+VFKR         +S      N+ S    +  
Sbjct: 1   MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKR---------NSNTAATTNRPSPINNAMR 60

Query: 61  CSANGQTSNAQNRTTSLPKTSPMKSSQVGTQKA-------SNSSIQASLVKSPGTTLKVS 120
            SA G T ++    +  P TSP +S+   T+ +       S+SS+Q S +KSP   L+  
Sbjct: 61  NSAIGSTKSSPPMRS--PLTSPNRSASSSTRSSMMKPALPSSSSVQRSTLKSP---LRDD 120

Query: 121 DSTDSQSRH-LVKAAVKEENDRHPTNESSDSEDDKPLSFRLKGKS-----NPPGKGLVPT 180
            S  ++ R+   KA    ++D        DSEDDKPLS RLK  S      P   G   T
Sbjct: 121 RSVVAKERNGFGKAPSVSKSD------DEDSEDDKPLSARLKLDSKEVTKQPSSSGRGST 180

Query: 181 VLKNKVEKSSADS------------DDEIPLSSKFHMKSSSRVSGGNQHGFDEKKPLISK 240
             +  V+KS+               D+  P+SS    K+S   S       ++K+PL++ 
Sbjct: 181 --QQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNN 240

Query: 241 VHQNGL---ISGDKLQKPGVISSKRPLPNADSHLPQFSSKKPKLSDSSTPL--------- 300
           + +NGL     G   Q P    +KRPL    S   Q S K+PKLS+ + P+         
Sbjct: 241 IDRNGLKPKTEGHSSQAP----AKRPLEKGSSS-NQSSVKRPKLSEPARPVKVEQGSHIS 300

Query: 301 ------GSKLVSVK--------AKQEVDDNDDHIPISQRMKKVAVSANKSLSSKKTVSKV 360
                 G  L + K         K++  D DDH+PI+ RMK  + S NKS S+K + SK+
Sbjct: 301 ATQDAKGKNLDASKPLRANQATVKEDNSDGDDHVPIASRMKSDS-SNNKSSSAKPSSSKM 360

Query: 361 VSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVKMI 420
           ++SS +   +K  K VK SKY KS+K LP SGDGQKKW +L HNGVIFPPPY+ HGVK++
Sbjct: 361 IASSSRTIAQKPNKWVKDSKYSKSSKSLP-SGDGQKKWKTLQHNGVIFPPPYKRHGVKIL 420

Query: 421 YKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
           ++GK VDLTPEQEEVATMFAVM++T+Y +KP+F+ENFWNDWRKLLGKNH+I++LDDCDFT
Sbjct: 421 FQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCDFT 480

Query: 481 PIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLFRG 540
           PIY+WY  EKE KKQMT EEK+ +KEEK+KQEEKYMWA+LDGV+E++GNFRVEPPGLFRG
Sbjct: 481 PIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPGLFRG 540

Query: 541 RGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINP 600
           RGEHPKMGKLK+RI P DITINIGK+APIPECPIPGERWKEV+HDNTVTWLAFW+DPINP
Sbjct: 541 RGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINP 600

Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660
           KEFKYVFLAASSSLKGQSDKEKYEKARKL ++I +IR AYTKDF +KD T+RQIAVATYL
Sbjct: 601 KEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYL 660

Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
           IDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE  V
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPLV 720

Query: 721 FKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
           +KAI QFQ+GKS TDDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML++ T+
Sbjct: 721 YKAIGQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKETR 780

Query: 781 DGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRA 840
           DGDV EK+V+YQ ANKEVAIICNHQRTVSKSHGAQ+ +L  KI ELR+ +KEL IDLDRA
Sbjct: 781 DGDVPEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDRA 840

Query: 841 KKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKINYL 884
           KKG+ PL  +DGK+KRNLTPE LEKKI QT  KIEK+ERDMQTKED+KTVALGTSKINY+
Sbjct: 841 KKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINYM 900

BLAST of CmaCh06G008570 vs. ExPASy Swiss-Prot
Match: P93119 (DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 4.3e-241
Identity = 487/824 (59.10%), Postives = 582/824 (70.63%), Query Frame = 0

Query: 99  SPGTTLKVSDSTDSQSRHLVKAAVKEENDRHPTNESSDSEDDKPLSFR-----LKGKSNP 158
           +PG T+         + ++++  +KEE      + S DS+DDKPLS +     L G S+ 
Sbjct: 4   NPGITV-----IKQNASNVMEKTLKEEGQSR--SNSEDSDDDKPLSHKLSSGALNGNSHH 63

Query: 159 PGKG---LVPTVLKNKVEKSSADSDDEIPLSSKFHMKSSSRVSGGNQHGFDEKKPLISKV 218
              G     P+   +   +     +DE+ LSS+  + + +  S       DE KPL S  
Sbjct: 64  IRMGSNLSCPSPYTSPKPRIIKGPEDEMLLSSELQLNAGA--SNAESSDSDESKPLAS-- 123

Query: 219 HQNGLISGDKLQKPGVISSKRPLPNADSHLPQFSSKKPKLSDSSTPLGSKLVSVKAKQEV 278
                    +L+ P    +KRPL    +  P   SKK KLS+ S P   KL +  A+QE 
Sbjct: 124 ---------ELEDPSSSLNKRPLIQPKNSDPS-PSKKAKLSEPSAPTNRKLKT--AEQEE 183

Query: 279 DDNDDHIPISQRMKKVAVSANKSLSSKKTVSKVVSSSMKKTFKKSKKPVKKSKYVKSTKL 338
              DD   IS R KK    A+K    KK     VS+S+ K        V  SK +K   +
Sbjct: 184 AAADDDPSISNRNKKSTTPASKVSDKKKRPD--VSASVNK--------VDFSKSLKVPLV 243

Query: 339 LPSSGDGQKKWTSLVHNGVIFPPPYQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDY 398
           L    +GQ     LVHNGVIFPP Y+ HGVKM+Y+   VDLTPEQEEVATMFAVM +T+Y
Sbjct: 244 LVKGRNGQ----PLVHNGVIFPPLYKPHGVKMLYRENPVDLTPEQEEVATMFAVMLETEY 303

Query: 399 MQKPRFKENFWNDWRKLLGKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEE 458
           M KP+F+ENF +DWRK+LG+ H+IQNL+DCDFTPIY+W+Q EKEKKKQM+T+EKKA+KE 
Sbjct: 304 MTKPKFRENFMSDWRKILGEKHIIQNLEDCDFTPIYEWHQREKEKKKQMSTDEKKAIKEG 363

Query: 459 KMK-QEEKYMWAILDGV-KEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIHPS-DITINIG 518
           K + ++EKYMWA++DG+ +EKVGNFRV    + +      K  ++K+ ++   DITINIG
Sbjct: 364 KDETRKEKYMWAVVDGMSREKVGNFRVGTTRVVQRSWRASKDPQVKKNVYSQIDITINIG 423

Query: 519 KDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYE 578
           KDAPIPE PIPGERWKE+RHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKG+  +EKYE
Sbjct: 424 KDAPIPEPPIPGERWKEIRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGKVTREKYE 483

Query: 579 KARKLKDYIKNIR-NAYTKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCC 638
           K+RKLKDYI+ IR  AYTKDF SKD  +RQIAVATYLIDKLALRAGNEKDDDEADTVGCC
Sbjct: 484 KSRKLKDYIEGIRAAAYTKDFASKDSKKRQIAVATYLIDKLALRAGNEKDDDEADTVGCC 543

Query: 639 TLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQSGKSGTDDLFDKLDT 698
           TLKVENV+   PN+L+F+FLGKDSI+Y+N VEVE  VF AI QF+SGK G DDLFD+LDT
Sbjct: 544 TLKVENVETKRPNILKFDFLGKDSIRYQNEVEVEPRVFSAIEQFRSGKEGGDDLFDQLDT 603

Query: 699 SKLNAHLKDLMPGLTAKVFRTYNASITLDDM-----LNRGTKDGDVAEKIVIYQHANKEV 758
           SKLNAHLK+LMPGLTAKVFRTYNASITLD+M     L+R TK GDVAEK+V+YQHANKEV
Sbjct: 604 SKLNAHLKELMPGLTAKVFRTYNASITLDEMVRCKLLSRETKGGDVAEKVVVYQHANKEV 663

Query: 759 AIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAKKGKPPLKDADGKQKRNL 818
           AIICNHQRTVSKSH AQM RLNEKI EL+ +LKEL+ DL R  KGKPPLK++DGK KRNL
Sbjct: 664 AIICNHQRTVSKSHSAQMVRLNEKIEELKGLLKELQEDLTRVNKGKPPLKNSDGKPKRNL 723

Query: 819 TPE----------------------VLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 878
             +                       + KKI+QTN KIEK+ERD +TKEDLKTVALGTSK
Sbjct: 724 NLKRYKGKLLKLIQKLRKWSETRRLSVTKKIAQTNTKIEKMERDKETKEDLKTVALGTSK 783

Query: 879 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           INYLDPRITVAWCKRH+VPIEKIFNKSLLAKFAWAMDV   FRF
Sbjct: 784 INYLDPRITVAWCKRHDVPIEKIFNKSLLAKFAWAMDVVLIFRF 790

BLAST of CmaCh06G008570 vs. ExPASy Swiss-Prot
Match: P07799 (DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=top1 PE=1 SV=2)

HSP 1 Score: 531.9 bits (1369), Expect = 1.3e-149
Identity = 346/857 (40.37%), Postives = 479/857 (55.89%), Query Frame = 0

Query: 107 SDSTDSQSRHLVKAAVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNK 166
           SDS     R   +    +      ++E SDS ++ PLS  L                 NK
Sbjct: 6   SDSVSLSIRRRQRRGSSKRISMKESDEESDSSENHPLSESL-----------------NK 65

Query: 167 VEKSSADSDDEIPLSSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGV 226
             KS +D DD IP+  +   ++SS+ +  N      KK   +KV  NG +   K      
Sbjct: 66  KSKSESDEDD-IPIRKR---RASSKKNMSNS---SSKKR--AKVMGNGGLKNGK------ 125

Query: 227 ISSKRPLPNADSHLPQFSSKKPKLSDSSTPLGSKLVSVKA-KQEVDDNDDHIPISQRMKK 286
              K  +   +    + +   PK    S   GSK  +  A K+E  D DD +P+      
Sbjct: 126 ---KTAVVKEEEDFNEIAKPSPKHKRVSKANGSKNGAKSAVKKEESDTDDSVPLR----- 185

Query: 287 VAVSANKSLSSKKTVSKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLV 346
            AVS       K  +    S++  ++    +   +  K+  S  +     D  +KWT+L 
Sbjct: 186 -AVSTVSLTPYKSELPSGASTTQNRSPNDEEDEDEDYKWWTSENI-----DDTQKWTTLE 245

Query: 347 HNGVIFPPPYQ--THGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWND 406
           HNGVIF PPY+     VK+IY G  V+L PE EEVA  +A M +TD+ + P F++NF+ D
Sbjct: 246 HNGVIFAPPYEPLPKNVKLIYDGNPVNLPPEAEEVAGFYAAMLETDHAKNPVFQDNFFRD 305

Query: 407 WRKLLGK---NHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMW 466
           + K+  +   NH I+    CDFT ++  ++ ++E+KK M  E+KKA+K++K ++EEKY W
Sbjct: 306 FLKVCDECNFNHNIKEFSKCDFTQMFHHFEQKREEKKSMPKEQKKAIKQKKDEEEEKYKW 365

Query: 467 AILDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGE 526
            ILDG KEKVGNFR+EPPGLFRGRG HPK G LKRR++P  ITINIG+  P+PE P+PG 
Sbjct: 366 CILDGRKEKVGNFRIEPPGLFRGRGSHPKTGSLKRRVYPEQITINIGEGVPVPE-PLPGH 425

Query: 527 RWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIR 586
           +W EV+HDNTVTWLA W++ IN    KYVFLAA SSLKGQSD +KYEK+RKLKDYI +IR
Sbjct: 426 QWAEVKHDNTVTWLATWHENIN-NNVKYVFLAAGSSLKGQSDLKKYEKSRKLKDYIDDIR 485

Query: 587 NAYTKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNL 646
             Y KD  S+    RQ   A YLID  ALRAGNEK +DEADTVGCC+L+ E+V   PP  
Sbjct: 486 KGYRKDLKSELTVERQRGTAMYLIDVFALRAGNEKGEDEADTVGCCSLRYEHVTLKPPRT 545

Query: 647 LEFNFLGKDSIKYENTVEVELPVFKAITQF-QSGKSGTDDLFDKLDTSKLNAHLKDLMPG 706
           + F+FLGKDSI+Y N VEV+  VFK +  F +  K   D +FD+L T+ LN +L  LM G
Sbjct: 546 VVFDFLGKDSIRYYNEVEVDPQVFKNLKIFKRPPKKEGDLIFDRLSTNSLNKYLTSLMDG 605

Query: 707 LTAKVFRTYNASITLDDMLNRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQM 766
           L+AKVFRTYNAS T+ + L +  K+  +A+KI+ Y  AN+ VAI+CNHQR+V+K+H  QM
Sbjct: 606 LSAKVFRTYNASYTMAEELKKMPKNLTLADKILFYNRANRTVAILCNHQRSVTKNHDVQM 665

Query: 767 SRLNEKISEL---RDILKELKIDLD-RAKKGKPPL------------------------- 826
            R  E+I  L   R  L+++ ++L+ +  K KP L                         
Sbjct: 666 ERFAERIKALQYQRMRLRKMMLNLEPKLAKSKPELLAKEEGITDSWIVKHHETLYELEKE 725

Query: 827 ----------------------------------------KDADGKQKR----NLTPEVL 884
                                                    DA+ K K+      + E L
Sbjct: 726 KIKKKFDRENEKLAAEDPKSVLPESELEVRLKAADELKKALDAELKSKKVDPGRSSMEQL 785

BLAST of CmaCh06G008570 vs. ExPASy Swiss-Prot
Match: Q54RC3 (DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 6.2e-147
Identity = 334/832 (40.14%), Postives = 477/832 (57.33%), Query Frame = 0

Query: 98  KSPGTTLKVSDSTDSQSRHLVKAAVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKG 157
           K P  +   SDS+DS S     +   + +    +++SS S+ D P    +    N P  G
Sbjct: 87  KKPVDSSSDSDSSDSSSDS--DSDSSDSDSDSSSSDSSSSDSDVPKKKPISKIPNKP-SG 146

Query: 158 LVPTVLKNKVEKSSADSDDEIPLSSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLIS 217
            V    KN  + ++  +     + SK   K     S  +    D           +   S
Sbjct: 147 TVSKTSKNSPKTTTTTTTTTTTVKSKPAPKVKPEQSSSDDSSSDSSD---DSSDDSSSDS 206

Query: 218 GDKLQKPGVISSKRPLPNADSHLPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHI 277
             + +KP    SK+P           + KK       T   +K   VK + EV   +   
Sbjct: 207 SSEEEKPKKKPSKKPA----------AVKKEITVKKETTTTTKKTPVKKEVEV-KKETKT 266

Query: 278 PISQRMKKVAVSANKSLSSKKTVSKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDG 337
             ++++KK   S +     K T  K  ++  +K  ++ +   ++ + V          D 
Sbjct: 267 TTTKKVKK-EESDDDGEDEKTTTKKKTTTRKRKLKEEEEGEEEEEEGVNKWWDKEDDEDD 326

Query: 338 QKKWTSLVHNGVIFPPPYQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFK 397
             KWT+L+HNG +FPP Y  HG+K +Y GK+V LTP+QEEVAT +A   +TD+++K  FK
Sbjct: 327 GTKWTTLIHNGPVFPPAYVPHGIKFMYDGKVVKLTPKQEEVATFYANYLETDHVKKQAFK 386

Query: 398 ENFWNDWRKLL--GKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQE 457
           +NF+ +++ LL   +  +++ +D CDF+ I+   +T+KE++K  + EEK A   EK K +
Sbjct: 387 DNFFKEFKGLLTSEQKEIVKKMDKCDFSHIHTHLKTKKEQRKARSKEEKDAETAEKKKIQ 446

Query: 458 EKYMWAILDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPEC 517
           EK+ +A +DG KEKVGN+ +EPPGLF GRG+HPK G LK+RI PSD+T+NIG+   IP  
Sbjct: 447 EKHGFATIDGFKEKVGNYMIEPPGLFLGRGQHPKTGMLKQRIMPSDVTLNIGEGEVIPPS 506

Query: 518 PIPGERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDY 577
           PIPGE+WKE+ H+NTVTWLAFW + +N   FKYV+LA SS +KGQ+D++K+E AR LK+ 
Sbjct: 507 PIPGEKWKEIVHNNTVTWLAFWRESVN-GGFKYVWLAPSSKIKGQADRKKFEVARGLKNC 566

Query: 578 IKNIRNAYTKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKA 637
           I +IR  Y K F  +   +RQ+AVA YLID LALR GNEK +D+ADTVGCC+L+VE++K 
Sbjct: 567 IGDIRKGYQKSFNDESTEKRQLAVALYLIDFLALRVGNEKGEDQADTVGCCSLRVEHIKL 626

Query: 638 VPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKD 697
              N +  +FLGKDS++Y NTV++    +K +  F  GK  TD LFD L  ++LN HLK 
Sbjct: 627 ESNNTITLDFLGKDSMRYLNTVQIREDAYKVLKTFVKGKKPTDLLFDSLTVTQLNNHLKK 686

Query: 698 LMPGLTAKVFRTYNASITLDDMLNR-GTKD-GDVAEKIVIYQHANKEVAIICNHQRTVSK 757
            M GL+AKVFRTYNASITL   L++  TK+   + EKI+ Y   N+EVAI+CNHQ+   K
Sbjct: 687 QMTGLSAKVFRTYNASITLQKELDKMDTKEFQSIDEKILFYNRCNREVAILCNHQKAPPK 746

Query: 758 SHGAQMSRLNEKISELRDILKELKIDLDRAKKGKPP------------------------ 817
           +    MS+++EKI ELRD+      DL R K GK P                        
Sbjct: 747 NLAETMSKIDEKIQELRDLQ-----DLCRIKIGKTPKSQQEDIESREAERLPKLIQLAIT 806

Query: 818 -LKDADGK--------------QKRNLTPEV---LEKKISQTNAKIEKIERDMQTKEDLK 877
             KD D +              +K    P+    +++KI++   +I+K E     K++LK
Sbjct: 807 NKKDKDKEKTDKEISFDVKAAFEKSRTLPDAKGKIKEKIAKYEEQIKKQEILKTGKDELK 866

Query: 878 TVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           TVALGTSKINYLDPRITVAWCK+++VP++KIF KSL  KF W+ DV  D+ F
Sbjct: 867 TVALGTSKINYLDPRITVAWCKKNKVPVDKIFTKSLRDKFPWS-DVGTDYIF 893

BLAST of CmaCh06G008570 vs. ExPASy TrEMBL
Match: A0A6J1L1R3 (DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111498332 PE=3 SV=1)

HSP 1 Score: 1722.2 bits (4459), Expect = 0.0e+00
Identity = 883/883 (100.00%), Postives = 883/883 (100.00%), Query Frame = 0

Query: 1   MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60
           MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ
Sbjct: 1   MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60

Query: 61  TSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRHLVKA 120
           TSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRHLVKA
Sbjct: 61  TSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRHLVKA 120

Query: 121 AVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSDDEIPL 180
           AVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSDDEIPL
Sbjct: 121 AVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSDDEIPL 180

Query: 181 SSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPNADSHL 240
           SSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPNADSHL
Sbjct: 181 SSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPNADSHL 240

Query: 241 PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKVAVSANKSLSSKKTV 300
           PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKVAVSANKSLSSKKTV
Sbjct: 241 PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKVAVSANKSLSSKKTV 300

Query: 301 SKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGV 360
           SKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGV
Sbjct: 301 SKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGV 360

Query: 361 KMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDDC 420
           KMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDDC
Sbjct: 361 KMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDDC 420

Query: 421 DFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGL 480
           DFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGL
Sbjct: 421 DFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGL 480

Query: 481 FRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDP 540
           FRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDP
Sbjct: 481 FRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDP 540

Query: 541 INPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVA 600
           INPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVA
Sbjct: 541 INPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVA 600

Query: 601 TYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVE 660
           TYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVE
Sbjct: 601 TYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVE 660

Query: 661 LPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNR 720
           LPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNR
Sbjct: 661 LPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNR 720

Query: 721 GTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDL 780
           GTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDL
Sbjct: 721 GTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDL 780

Query: 781 DRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKI 840
           DRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKI
Sbjct: 781 DRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKI 840

Query: 841 NYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           NYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 NYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 883

BLAST of CmaCh06G008570 vs. ExPASy TrEMBL
Match: A0A6J1FIM6 (DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111446061 PE=3 SV=1)

HSP 1 Score: 1683.3 bits (4358), Expect = 0.0e+00
Identity = 863/884 (97.62%), Postives = 872/884 (98.64%), Query Frame = 0

Query: 1   MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60
           MAVETFGKAP FDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCS NGQ
Sbjct: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60

Query: 61  TSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRHLVKA 120
           +SNAQNRTTSLPKTSPMKSS+VGTQKASNSSIQA LVKSPGTTLKVSDSTDSQS+HLVKA
Sbjct: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTDSQSKHLVKA 120

Query: 121 AVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSDDEIPL 180
           AVKEE+DRHPTNE+SDSEDDKPLSFRLKG SNPPGKGLVPTVLKNKVEKSS DSDD IPL
Sbjct: 121 AVKEESDRHPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDGIPL 180

Query: 181 SSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPNADSH- 240
           SSKFHMKS+SRVSGGNQHGFDEKKPLISKVHQNG  SGDKLQKPGVISSKRPLPNADSH 
Sbjct: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSGDKLQKPGVISSKRPLPNADSHS 240

Query: 241 LPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKVAVSANKSLSSKKT 300
            PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDD+DDHIPISQRMKKVA+SANKSLSSKKT
Sbjct: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVALSANKSLSSKKT 300

Query: 301 VSKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360
           VSKVVSSSMKKT KKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG
Sbjct: 301 VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360

Query: 361 VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDD 420
           VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKP+FKENFWNDWRKLLGKNHVIQNLDD
Sbjct: 361 VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 420

Query: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480
           CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG
Sbjct: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480

Query: 481 LFRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540
           LFRGRGEHPKMGKLKRRIHP DITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND
Sbjct: 481 LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540

Query: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600
           PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV
Sbjct: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600

Query: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660
           ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV
Sbjct: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660

Query: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720
           ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN
Sbjct: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720

Query: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780
           RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID
Sbjct: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780

Query: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840
           LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK
Sbjct: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840

Query: 841 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884

BLAST of CmaCh06G008570 vs. ExPASy TrEMBL
Match: A0A6J1KZE0 (DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1)

HSP 1 Score: 1521.9 bits (3939), Expect = 0.0e+00
Identity = 787/889 (88.53%), Postives = 826/889 (92.91%), Query Frame = 0

Query: 1   MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAK----QRLNKLSGRQGSDVCS 60
           MAVETFGKA  FD+DDEDNMPIVFKRSSASRQNQL+SE K    QRLN+LSGR GSD CS
Sbjct: 1   MAVETFGKATMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQTSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRH 120
            NGQ+SNAQ R TSLPKTSPM+  ++G+QKAS SSIQ  LVKS GTTLKVSDS DS+ + 
Sbjct: 61  TNGQSSNAQKRMTSLPKTSPMR-PEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVKAAVKEENDR-HPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSD 180
           LV AAVKEEND  HP  E+SDSEDDKPLS RLKG SN P KG +PTV KNK++KSS DSD
Sbjct: 121 LVNAAVKEENDSIHPAQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEIPLSSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPN 240
           DE+PLSSKF MKS+S V GG QHGFDEKKPL SKVHQNG  S DKLQKP V+SSKRPLPN
Sbjct: 181 DEVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKPAVVSSKRPLPN 240

Query: 241 ADSH-LPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKVAVSANKSL 300
           ADSH   Q SSKKPKLSDSSTPL +  VS+KAKQEV D+DDHIPISQRM K++ + NKS 
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRM-KMSAAVNKSS 300

Query: 301 SSKKTVSKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPP 360
            SKKTVSKV SSS+KKT KKSKKPVKKSKYVKSTKLLPSSGDGQKKWT+LVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVI 420
           YQTHGVK+++KGK VDLTPEQEEVATMFAVMKDTDYMQKP+FKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEK+KQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RI PSDITINIGKDAPIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540

Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTR 600
           AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKAR LKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILK 780
           DDMLNRGTKDG+V+EKIV YQHANKEVAIICNHQRTVSKSHGAQM+RLNEKISELRDILK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVA 840
           ELKIDLDRAKKGKPPLK +DGKQKRNLTPE LEKKISQTNAKIEKIERDM+TKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of CmaCh06G008570 vs. ExPASy TrEMBL
Match: A0A6J1EWI0 (DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1)

HSP 1 Score: 1519.6 bits (3933), Expect = 0.0e+00
Identity = 786/889 (88.41%), Postives = 826/889 (92.91%), Query Frame = 0

Query: 1   MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAK----QRLNKLSGRQGSDVCS 60
           MAVETFGKAP FD+DDEDNMPIVFKRSSASRQNQL+SE K    QRLN+LSGR GSD CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQTSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRH 120
           ANGQ+SNAQ R TSLPKTSPM+  ++G+QKA+ SSIQ  LVKS GTTLKVSDS DS+ + 
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMR-PEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVKAAVKEENDR-HPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSD 180
           L  AAVKEEND  HPT E+SDSEDDKPLS RLKG SN P KG +PTV KNK++KSS DSD
Sbjct: 121 LANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEIPLSSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPN 240
           DE+PLSSKF MKS+S V GG QHGF EKKPL SKVHQNG  S DKLQKP V+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240

Query: 241 ADSH-LPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKVAVSANKSL 300
           ADSH   Q SSKKPKLSDSSTPL +  VS+KAKQEV D+DDHIPISQRM K++ + NKS 
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRM-KMSAAVNKSS 300

Query: 301 SSKKTVSKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPP 360
            SKKTVSKV SSS+KKT KKSKKPVKKSKYVKSTKLLPSSGDGQKKWT+LVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVI 420
           YQTHGVK+++KGK VDLT EQEEVATMFAVMKDTDYMQKP+FKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEK+KQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RI PSDITINIGKDAPIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540

Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTR 600
           AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKAR LKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILK 780
           DDMLNRGTKDG+V+EKIV YQHANKEVAIICNHQRTVSKSHGAQM+RLNEKISELRDILK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVA 840
           ELKIDLDRAKKGKPPLK +DGKQKRNLTPE LEKKISQTNAKIEKIERDM+TKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of CmaCh06G008570 vs. ExPASy TrEMBL
Match: A0A1S3BM72 (DNA topoisomerase I OS=Cucumis melo OX=3656 GN=LOC103491068 PE=3 SV=1)

HSP 1 Score: 1505.0 bits (3895), Expect = 0.0e+00
Identity = 781/890 (87.75%), Postives = 821/890 (92.25%), Query Frame = 0

Query: 1   MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAK----QRLNKLSGRQGSDVCS 60
           MAVETFGKAP FD+DDEDNMPIVFKRSSASRQNQLDSE K    QRLNKL GRQGSD+CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60

Query: 61  ANGQTSNAQNRTTSLPKTSPMKSSQVGTQKASNSS-IQASLVKSPGTTLKVSDSTDSQSR 120
            NGQ+SN Q R TSLPKTSPM+ S++G+QKA  SS IQ  L KS G +LK SDS DS+ +
Sbjct: 61  TNGQSSNNQKRMTSLPKTSPMR-SEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120

Query: 121 HLVKAAVKEEN-DRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADS 180
           +LV AAVKEEN   +P+ E+SDSEDDKPLS RLKG SN P KGLV T+ KNKV+KSS DS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180

Query: 181 DDEIPLSSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLP 240
           DDE+PLSSKF MK +S   G  Q GFDEKKPL+ KVHQNG  S DKLQKP V+SSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240

Query: 241 NADSHLP-QFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKVAVSANKS 300
           N DSH P QFS+KKPKLSDSS+PL SKLVS+KAKQEVDD+DD IPISQR+KK   + NKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300

Query: 301 LSSKKTVSKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPP 360
            SSK TVSKVVSSS+KKTFKKSKKPVKKSKYVKSTKLLPS+GDGQKKWT+LVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHV 420
           PYQTHGVKM+YKGK VDLTP+QEEVATMFAVMKDTDYM KP+FKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNF 480
           IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEK+KQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480

Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTW 540
           RVEPPGLFRGRGEHPKMGKLKRRI PSDITINIGK APIPECPIPGERWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540

Query: 541 LAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPT 600
           LAFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKAR LKDYIKNIRNAYTKDFTSKDPT
Sbjct: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPT 600

Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKY 660
           RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+Y
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRY 660

Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
           ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASIT 720

Query: 721 LDDMLNRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDIL 780
           LDDMLNRGTKDG+V+EKIV YQHANKEVAIICNHQRTVSKSHGAQM+RLNEKISELRDIL
Sbjct: 721 LDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780

Query: 781 KELKIDLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTV 840
           KELKIDLDRAKKGKPPLK +DGKQKRNLTPE LEKKISQTNAKIEKIERDM+TKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840

Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of CmaCh06G008570 vs. NCBI nr
Match: XP_023005283.1 (DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023005284.1 DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023005285.1 DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023005286.1 DNA topoisomerase 1 beta-like [Cucurbita maxima])

HSP 1 Score: 1722.2 bits (4459), Expect = 0.0e+00
Identity = 883/883 (100.00%), Postives = 883/883 (100.00%), Query Frame = 0

Query: 1   MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60
           MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ
Sbjct: 1   MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60

Query: 61  TSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRHLVKA 120
           TSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRHLVKA
Sbjct: 61  TSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRHLVKA 120

Query: 121 AVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSDDEIPL 180
           AVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSDDEIPL
Sbjct: 121 AVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSDDEIPL 180

Query: 181 SSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPNADSHL 240
           SSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPNADSHL
Sbjct: 181 SSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPNADSHL 240

Query: 241 PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKVAVSANKSLSSKKTV 300
           PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKVAVSANKSLSSKKTV
Sbjct: 241 PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKVAVSANKSLSSKKTV 300

Query: 301 SKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGV 360
           SKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGV
Sbjct: 301 SKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGV 360

Query: 361 KMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDDC 420
           KMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDDC
Sbjct: 361 KMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDDC 420

Query: 421 DFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGL 480
           DFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGL
Sbjct: 421 DFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGL 480

Query: 481 FRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDP 540
           FRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDP
Sbjct: 481 FRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDP 540

Query: 541 INPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVA 600
           INPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVA
Sbjct: 541 INPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVA 600

Query: 601 TYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVE 660
           TYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVE
Sbjct: 601 TYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVE 660

Query: 661 LPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNR 720
           LPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNR
Sbjct: 661 LPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNR 720

Query: 721 GTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDL 780
           GTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDL
Sbjct: 721 GTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDL 780

Query: 781 DRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKI 840
           DRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKI
Sbjct: 781 DRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKI 840

Query: 841 NYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           NYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 NYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 883

BLAST of CmaCh06G008570 vs. NCBI nr
Match: XP_022940477.1 (DNA topoisomerase 1 alpha-like [Cucurbita moschata] >XP_022940487.1 DNA topoisomerase 1 alpha-like [Cucurbita moschata] >XP_022940496.1 DNA topoisomerase 1 alpha-like [Cucurbita moschata])

HSP 1 Score: 1683.3 bits (4358), Expect = 0.0e+00
Identity = 863/884 (97.62%), Postives = 872/884 (98.64%), Query Frame = 0

Query: 1   MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60
           MAVETFGKAP FDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCS NGQ
Sbjct: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60

Query: 61  TSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRHLVKA 120
           +SNAQNRTTSLPKTSPMKSS+VGTQKASNSSIQA LVKSPGTTLKVSDSTDSQS+HLVKA
Sbjct: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTDSQSKHLVKA 120

Query: 121 AVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSDDEIPL 180
           AVKEE+DRHPTNE+SDSEDDKPLSFRLKG SNPPGKGLVPTVLKNKVEKSS DSDD IPL
Sbjct: 121 AVKEESDRHPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDGIPL 180

Query: 181 SSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPNADSH- 240
           SSKFHMKS+SRVSGGNQHGFDEKKPLISKVHQNG  SGDKLQKPGVISSKRPLPNADSH 
Sbjct: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSGDKLQKPGVISSKRPLPNADSHS 240

Query: 241 LPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKVAVSANKSLSSKKT 300
            PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDD+DDHIPISQRMKKVA+SANKSLSSKKT
Sbjct: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVALSANKSLSSKKT 300

Query: 301 VSKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360
           VSKVVSSSMKKT KKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG
Sbjct: 301 VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360

Query: 361 VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDD 420
           VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKP+FKENFWNDWRKLLGKNHVIQNLDD
Sbjct: 361 VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 420

Query: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480
           CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG
Sbjct: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480

Query: 481 LFRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540
           LFRGRGEHPKMGKLKRRIHP DITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND
Sbjct: 481 LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540

Query: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600
           PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV
Sbjct: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600

Query: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660
           ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV
Sbjct: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660

Query: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720
           ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN
Sbjct: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720

Query: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780
           RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID
Sbjct: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780

Query: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840
           LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK
Sbjct: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840

Query: 841 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884

BLAST of CmaCh06G008570 vs. NCBI nr
Match: XP_023520897.1 (DNA topoisomerase 1 alpha-like [Cucurbita pepo subsp. pepo] >XP_023520898.1 DNA topoisomerase 1 alpha-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1680.6 bits (4351), Expect = 0.0e+00
Identity = 864/885 (97.63%), Postives = 873/885 (98.64%), Query Frame = 0

Query: 1   MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60
           MA+ETFGKAP FDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ
Sbjct: 1   MAIETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60

Query: 61  TSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRHLVKA 120
           +SNAQNRTTSLPKTSPMKSS+VGTQKASNSS QA  VKSPGTTLKVSDSTDSQS+HLVKA
Sbjct: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTDSQSKHLVKA 120

Query: 121 AVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSDDEIPL 180
           AVKEE+DRHPTNESSDSEDDKPLSFRLKG SNPPGKGLVPTVLKNKVEKSS DSDDEIPL
Sbjct: 121 AVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180

Query: 181 SSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPNADSH- 240
           SSKFHMKS+SRVSGGNQHGFDEKKPLISKVHQNGL SGDKLQKPGVISSKRPLPNADSH 
Sbjct: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240

Query: 241 LPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKK-VAVSANKSLSSKK 300
            PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDD+DDHIPISQRMKK VAVSANKSLSSKK
Sbjct: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVVAVSANKSLSSKK 300

Query: 301 TVSKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 360
           TVSKVVSSSMKKT KKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH
Sbjct: 301 TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 360

Query: 361 GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLD 420
           GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKP+FKENFWNDWRKLLGKNHVIQNLD
Sbjct: 361 GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD 420

Query: 421 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 480
           DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP
Sbjct: 421 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 480

Query: 481 GLFRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 540
           GLFRGRGEHPKMGKLKRRIHP DITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN
Sbjct: 481 GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 540

Query: 541 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 600
           DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA
Sbjct: 541 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 600

Query: 601 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 660
           VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE
Sbjct: 601 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 660

Query: 661 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 720
           VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML
Sbjct: 661 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 720

Query: 721 NRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 780
           NRG+KDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI
Sbjct: 721 NRGSKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 780

Query: 781 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 840
           DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS
Sbjct: 781 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 840

Query: 841 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 885

BLAST of CmaCh06G008570 vs. NCBI nr
Match: KAG6596930.1 (DNA topoisomerase 1 alpha, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028410.1 DNA topoisomerase 1 alpha [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1680.6 bits (4351), Expect = 0.0e+00
Identity = 862/884 (97.51%), Postives = 871/884 (98.53%), Query Frame = 0

Query: 1   MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60
           MAVETFGKAP FDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCS NGQ
Sbjct: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60

Query: 61  TSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRHLVKA 120
           +SNAQNRTTSLPKTSPMKSS+VGTQKASNSSIQA LVKSPGTTL VSDSTDSQS+HLV A
Sbjct: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLTVSDSTDSQSKHLVMA 120

Query: 121 AVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSDDEIPL 180
           +VKEE+DRHPTNESSDSEDDKPLSFRLKG SNPPGKGLVPTVLKNKVEKSS DSDDEIPL
Sbjct: 121 SVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180

Query: 181 SSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPNADSH- 240
           SSKFHMKS+SRVSGGNQHGFDEKKPLISKVHQNGL SGDKLQKPGVISSKRPLPNADSH 
Sbjct: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240

Query: 241 LPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKVAVSANKSLSSKKT 300
            PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDD+DDHIPISQRMKKVAVSANK LSSKKT
Sbjct: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVAVSANKPLSSKKT 300

Query: 301 VSKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360
           VSKVVSSSMKKT KKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG
Sbjct: 301 VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360

Query: 361 VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDD 420
           VK+IYKGKLVDLTPEQEEVATMFAVMKDTDYMQKP+FKENFWNDWRKLLGKNHVIQNLDD
Sbjct: 361 VKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 420

Query: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480
           CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG
Sbjct: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480

Query: 481 LFRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540
           LFRGRGEHPKMGKLKRRIHP DITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND
Sbjct: 481 LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540

Query: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600
           PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV
Sbjct: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600

Query: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660
           ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV
Sbjct: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660

Query: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720
           ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN
Sbjct: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720

Query: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780
           RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID
Sbjct: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780

Query: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840
           LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK
Sbjct: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840

Query: 841 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884

BLAST of CmaCh06G008570 vs. NCBI nr
Match: XP_023553297.1 (DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1524.6 bits (3946), Expect = 0.0e+00
Identity = 787/889 (88.53%), Postives = 828/889 (93.14%), Query Frame = 0

Query: 1   MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAK----QRLNKLSGRQGSDVCS 60
           MAVETFGKAP FD+DDEDNMPIVFKRSSASRQNQL+SE K    QRLN+LSGR GSD CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQTSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRH 120
           ANGQ+SNAQ R TSLPKTSPM+  ++G+QKAS SSIQ  LVKS GTTLKVSDS DS+ + 
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMR-PEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVKAAVKEENDR-HPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSD 180
           L  AAVKEEND  HPT ++SDSEDDKPLS RLKG SN P KG +PTV KNK++KSS DSD
Sbjct: 121 LANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEIPLSSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPN 240
           DE+PLSSKF MKS+S V GG QHGFDEKKPL SKVHQNG  S DKLQKP V+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240

Query: 241 ADSH-LPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKVAVSANKSL 300
           ADSH   Q SSKKPKLSDSSTPL +  VS+KAKQEV D+DDHIPISQRM K++ + NKS 
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRM-KMSAAVNKSS 300

Query: 301 SSKKTVSKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPP 360
            SKKTVSKV SSS+KKT KKSKKPVKKSKYVKSTKLLPSSGDGQKKWT+LVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVI 420
           YQTHGVK+++KGK VDLTPEQEEVATMFAVMKDTDYMQKP+FKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEK+KQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RI PSDITINIGKDAPIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540

Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTR 600
           AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKAR LKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILK 780
           DDMLNRGTKDG+V+EKIV YQHANKEVAIICNHQRTVSKSHGAQM+RLNEKISELRDI+K
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780

Query: 781 ELKIDLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVA 840
           ELKIDLDRAKKGKPPLK +DGKQKRNLTPE LEKKISQTNAKIEKIERDM+TKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of CmaCh06G008570 vs. TAIR 10
Match: AT5G55300.1 (DNA topoisomerase I alpha )

HSP 1 Score: 1015.4 bits (2624), Expect = 2.8e-296
Identity = 575/939 (61.24%), Postives = 678/939 (72.20%), Query Frame = 0

Query: 1   MAVETFGKAPTFDE---DDEDNMPIVFKRSS--ASRQNQLDSEAKQR----LNKLSGRQG 60
           M  ET  K P  D    D +D+ P+ FKR++  AS  NQ  S +++       K+S  + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDVCSANGQTSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTD 120
           S V S NG        TT   KTS +KSS        +SS +AS  KSP      S   D
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSSM------PSSSSKASPAKSPLRNDMPSTVKD 120

Query: 121 SQSRHLVKAAVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNK----- 180
                  ++  K E++        DSEDD+PLS  L G   P     V +    K     
Sbjct: 121 RSQLQKDQSECKIEHE--------DSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 --VEKSSA----DSDDEIPLSSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDK 240
             ++++S     +SDDE P+SS F  K  S +SGGNQ   DEKKPL+ K+HQNG    ++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPGVISSKRPLPNADSHLPQFSSKKPKLSDSSTPL--------------GSKLVS--V 300
           +   G +  KRPL   +S   Q S KK K+S S T +              G  LVS  +
Sbjct: 241 VPN-GKVLGKRPL-EKNSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPKM 300

Query: 301 KAKQ-------EVDDNDDHIPISQRMKKVAVSANKSLSSKKTVS-------------KVV 360
           KAKQ         DD+DD +PIS+R K  + ++N S +  K V               VV
Sbjct: 301 KAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNVV 360

Query: 361 SSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVKMIY 420
           S   +   K +KK  K SKY  S+K  PSSGDGQKKWT+LVHNGVIFPPPY+ HG+K++Y
Sbjct: 361 SPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKILY 420

Query: 421 KGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDDCDFTP 480
           KGK VDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDCDFTP
Sbjct: 421 KGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFTP 480

Query: 481 IYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLFRGR 540
           IY+W+  EKEKKKQM+TEEKKALKEEKMKQEEKYMWA++DGVKEK+GNFRVEPPGLFRGR
Sbjct: 481 IYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRGR 540

Query: 541 GEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINPK 600
           GEHPKMGKLK+RIHP +IT+NIGK APIPECPI GERWKEV+HDNTVTWLAFW DPINPK
Sbjct: 541 GEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINPK 600

Query: 601 EFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLI 660
           EFKYVFL A SSLKG SDKEKYEKAR L D+I NIR  YTK+FT+KD   RQIAVATYLI
Sbjct: 601 EFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYLI 660

Query: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
           DKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+
Sbjct: 661 DKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVY 720

Query: 721 KAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
           KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKD
Sbjct: 721 KAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKD 780

Query: 781 GDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAK 840
           GDV +KIV+YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LDRAK
Sbjct: 781 GDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAK 840

Query: 841 KGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKINYLD 884
           KGKPPL+ +DGK+ R+L P   EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLD
Sbjct: 841 KGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLD 900

BLAST of CmaCh06G008570 vs. TAIR 10
Match: AT5G55310.1 (DNA topoisomerase 1 beta )

HSP 1 Score: 1013.8 bits (2620), Expect = 8.2e-296
Identity = 571/940 (60.74%), Postives = 687/940 (73.09%), Query Frame = 0

Query: 1   MAVETFGKAPT------FDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDV 60
           MA E F K         ++++DED++P+VFKR         +S      N+ S    +  
Sbjct: 1   MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKR---------NSNTAATTNRPSPINNAMR 60

Query: 61  CSANGQTSNAQNRTTSLPKTSPMKSSQVGTQKA-------SNSSIQASLVKSPGTTLKVS 120
            SA G T ++    +  P TSP +S+   T+ +       S+SS+Q S +KSP   L+  
Sbjct: 61  NSAIGSTKSSPPMRS--PLTSPNRSASSSTRSSMMKPALPSSSSVQRSTLKSP---LRDD 120

Query: 121 DSTDSQSRH-LVKAAVKEENDRHPTNESSDSEDDKPLSFRLKGKS-----NPPGKGLVPT 180
            S  ++ R+   KA    ++D        DSEDDKPLS RLK  S      P   G   T
Sbjct: 121 RSVVAKERNGFGKAPSVSKSD------DEDSEDDKPLSARLKLDSKEVTKQPSSSGRGST 180

Query: 181 VLKNKVEKSSADS------------DDEIPLSSKFHMKSSSRVSGGNQHGFDEKKPLISK 240
             +  V+KS+               D+  P+SS    K+S   S       ++K+PL++ 
Sbjct: 181 --QQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNN 240

Query: 241 VHQNGL---ISGDKLQKPGVISSKRPLPNADSHLPQFSSKKPKLSDSSTPL--------- 300
           + +NGL     G   Q P    +KRPL    S   Q S K+PKLS+ + P+         
Sbjct: 241 IDRNGLKPKTEGHSSQAP----AKRPLEKGSSS-NQSSVKRPKLSEPARPVKVEQGSHIS 300

Query: 301 ------GSKLVSVK--------AKQEVDDNDDHIPISQRMKKVAVSANKSLSSKKTVSKV 360
                 G  L + K         K++  D DDH+PI+ RMK  + S NKS S+K + SK+
Sbjct: 301 ATQDAKGKNLDASKPLRANQATVKEDNSDGDDHVPIASRMKSDS-SNNKSSSAKPSSSKM 360

Query: 361 VSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVKMI 420
           ++SS +   +K  K VK SKY KS+K LP SGDGQKKW +L HNGVIFPPPY+ HGVK++
Sbjct: 361 IASSSRTIAQKPNKWVKDSKYSKSSKSLP-SGDGQKKWKTLQHNGVIFPPPYKRHGVKIL 420

Query: 421 YKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
           ++GK VDLTPEQEEVATMFAVM++T+Y +KP+F+ENFWNDWRKLLGKNH+I++LDDCDFT
Sbjct: 421 FQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCDFT 480

Query: 481 PIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLFRG 540
           PIY+WY  EKE KKQMT EEK+ +KEEK+KQEEKYMWA+LDGV+E++GNFRVEPPGLFRG
Sbjct: 481 PIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPGLFRG 540

Query: 541 RGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINP 600
           RGEHPKMGKLK+RI P DITINIGK+APIPECPIPGERWKEV+HDNTVTWLAFW+DPINP
Sbjct: 541 RGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINP 600

Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660
           KEFKYVFLAASSSLKGQSDKEKYEKARKL ++I +IR AYTKDF +KD T+RQIAVATYL
Sbjct: 601 KEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYL 660

Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
           IDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE  V
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPLV 720

Query: 721 FKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
           +KAI QFQ+GKS TDDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML++ T+
Sbjct: 721 YKAIGQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKETR 780

Query: 781 DGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRA 840
           DGDV EK+V+YQ ANKEVAIICNHQRTVSKSHGAQ+ +L  KI ELR+ +KEL IDLDRA
Sbjct: 781 DGDVPEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDRA 840

Query: 841 KKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKINYL 884
           KKG+ PL  +DGK+KRNLTPE LEKKI QT  KIEK+ERDMQTKED+KTVALGTSKINY+
Sbjct: 841 KKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINYM 900

BLAST of CmaCh06G008570 vs. TAIR 10
Match: AT5G55300.2 (DNA topoisomerase I alpha )

HSP 1 Score: 1010.0 bits (2610), Expect = 1.2e-294
Identity = 573/936 (61.22%), Postives = 675/936 (72.12%), Query Frame = 0

Query: 1   MAVETFGKAPTFDE---DDEDNMPIVFKRSS--ASRQNQLDSEAKQR----LNKLSGRQG 60
           M  ET  K P  D    D +D+ P+ FKR++  AS  NQ  S +++       K+S  + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDVCSANGQTSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTD 120
           S V S NG        TT   KTS +KSS        +SS +AS  KSP      S   D
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSSM------PSSSSKASPAKSPLRNDMPSTVKD 120

Query: 121 SQSRHLVKAAVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNK----- 180
                  ++  K E++        DSEDD+PLS  L G   P     V +    K     
Sbjct: 121 RSQLQKDQSECKIEHE--------DSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 --VEKSSA----DSDDEIPLSSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDK 240
             ++++S     +SDDE P+SS F  K  S +SGGNQ   DEKKPL+ K+HQNG    ++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPGVISSKRPLPNADSHLPQFSSKKPKLSDSSTPL--------------GSKLVS--V 300
           +   G +  KRPL   +S   Q S KK K+S S T +              G  LVS  +
Sbjct: 241 VPN-GKVLGKRPL-EKNSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPKM 300

Query: 301 KAKQ-------EVDDNDDHIPISQRMKKVAVSANKSLSSKKTVS-------------KVV 360
           KAKQ         DD+DD +PIS+R K  + ++N S +  K V               VV
Sbjct: 301 KAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNVV 360

Query: 361 SSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVKMIY 420
           S   +   K +KK  K SKY  S+K  PSSGDGQKKWT+LVHNGVIFPPPY+ HG+K++Y
Sbjct: 361 SPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKILY 420

Query: 421 KGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDDCDFTP 480
           KGK VDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDCDFTP
Sbjct: 421 KGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFTP 480

Query: 481 IYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLFRGR 540
           IY+W+  EKEKKKQM+TEEKKALKEEKMKQEEKYMWA++DGVKEK+GNFRVEPPGLFRGR
Sbjct: 481 IYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRGR 540

Query: 541 GEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINPK 600
           GEHPKMGKLK+RIHP +IT+NIGK APIPECPI GERWKEV+HDNTVTWLAFW DPINPK
Sbjct: 541 GEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINPK 600

Query: 601 EFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLI 660
           EFKYVFL A SSLKG SDKEKYEKAR L D+I NIR  YTK+FT+KD   RQIAVATYLI
Sbjct: 601 EFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYLI 660

Query: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
           DKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+
Sbjct: 661 DKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVY 720

Query: 721 KAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
           KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKD
Sbjct: 721 KAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKD 780

Query: 781 GDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAK 840
           GDV +KIV+YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LDRAK
Sbjct: 781 GDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAK 840

Query: 841 KGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKINYLD 881
           KGKPPL+ +DGK+ R+L P   EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLD
Sbjct: 841 KGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLD 900

BLAST of CmaCh06G008570 vs. TAIR 10
Match: AT5G55300.3 (DNA topoisomerase I alpha )

HSP 1 Score: 427.2 bits (1097), Expect = 3.3e-119
Identity = 208/271 (76.75%), Postives = 244/271 (90.04%), Query Frame = 0

Query: 613 NEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQS 672
           ++ DDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+KAI QFQ+
Sbjct: 291 DDDDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQA 350

Query: 673 GKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAEKIV 732
           GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDGDV +KIV
Sbjct: 351 GKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIV 410

Query: 733 IYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAKKGKPPLKD 792
           +YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LDRAKKGKPPL+ 
Sbjct: 411 VYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEG 470

Query: 793 ADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKINYLDPRITVAWC 852
           +DGK+ R+L P   EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAWC
Sbjct: 471 SDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWC 530

Query: 853 KRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           KRHEVPIEKIF KSLL KFAWAMDV+P++RF
Sbjct: 531 KRHEVPIEKIFTKSLLEKFAWAMDVEPEYRF 561

HSP 2 Score: 88.2 bits (217), Expect = 3.6e-17
Identity = 108/312 (34.62%), Postives = 150/312 (48.08%), Query Frame = 0

Query: 1   MAVETFGKAPTFDE---DDEDNMPIVFKRSS--ASRQNQLDSEAKQR----LNKLSGRQG 60
           M  ET  K P  D    D +D+ P+ FKR++  AS  NQ  S +++       K+S  + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDVCSANGQTSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTD 120
           S V S NG        TT   KTS +KSS        +SS +AS  KSP      S   D
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSSM------PSSSSKASPAKSPLRNDMPSTVKD 120

Query: 121 SQSRHLVKAAVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNK----- 180
                  ++  K E++        DSEDD+PLS  L G   P     V +    K     
Sbjct: 121 RSQLQKDQSECKIEHE--------DSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 --VEKSSA----DSDDEIPLSSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDK 240
             ++++S     +SDDE P+SS F  K  S +SGGNQ   DEKKPL+ K+HQNG    ++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPGVISSKRPLPNADSHLPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPIS 293
           +   G +  KRPL   +S   Q S KK K+S S T +  K  SV  K+E+DD    + +S
Sbjct: 241 VPN-GKVLGKRPL-EKNSSADQSSLKKAKISASPTSVKMKQDSV--KKEIDDK-GRVLVS 283

BLAST of CmaCh06G008570 vs. TAIR 10
Match: AT4G26701.1 (DNA binding;DNA topoisomerase type Is )

HSP 1 Score: 131.0 bits (328), Expect = 4.9e-30
Identity = 61/68 (89.71%), Postives = 64/68 (94.12%), Query Frame = 0

Query: 816 KIEKIERDMQTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAM 875
           KIEK+ERDMQTKEDLKTVALGTSKINY+DPRITVAWCKRHE PIEKIFNKSLL KFAWAM
Sbjct: 2   KIEKMERDMQTKEDLKTVALGTSKINYMDPRITVAWCKRHEAPIEKIFNKSLLEKFAWAM 61

Query: 876 DVDPDFRF 884
           DV+P F F
Sbjct: 62  DVEPHFTF 69