CmaCh05G010620 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh05G010620
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionWAT1-related protein
LocationCma_Chr05: 8438982 .. 8441663 (+)
RNA-Seq ExpressionCmaCh05G010620
SyntenyCmaCh05G010620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGGAGATATCACAGCAATCATGGGGATGGTTATATTGCAATTTTGCTACGCTGGTTTAAGCATTACCTCAAAGCTAGCGATGCAATCAGGAATGAGCCCTCTTGTTCTCCTCACTTACAGGCAATTTTTTGGCACTTTGGCCATTGCTCCCTTTGCTTTCTTTACTGAAAGGTACTCAAAATGAATGAATCTTTGTTATGGGTATCAATTTCTGGGTTTGAAAAGATGATCAAGAACAATGAATGGATCTTTCTTGGTTCTTGTTCTTTATTTTGTTTCTGTTTCTTTGGCAGGAAAACAAGGCCAAAGATCACTTTCCCTGTTCTTGTTCAAATCTTTATCTGTTCTTTATCTGGGTTAGTCAAATGGGTTGTTGTCAAGTTTTTAAATTTTGGAACTTTTTTAGAATTGAAAGGCATTCTATGAATGTTTTGTGCAGAGCCACAGCGAACCAGATATTCTTCTTTGTTGGGTTGAAATGCACAAACCCAACCGTTTCAAGTGCTATGGCTAATGTTCTTCCTGCTGCTACTTTTATTCTTGCTGTTGTTTTCAGGTTAGGTTTTCAAGCAGCCATGAGTATTTTTCTAGTAGATATTGTCTGCTTTAGCCTCTTATTTATCGATGTTAGCCTCATAGTTTTAAAATGTGTCTACTAGGGAGAGGTATTATGAGATCCAATATTGGTTGGAGAGAGGAACAAAACATTTTTTTTTAGAAAGGTGTGACTTCTATCTAGTAGATATGGTTTAAAATTGTGAGGCCGACTGGTATATGTAAGGGGCTAAAGTGGACAATATTTGCTAGTGGTGGGTTTGAGGTGTTAGAAATGGTTTAAGAGCTAGACATTTGGTGGTGTGCCAGCGAGGATGTTAGGCTCTCAAGGGGTAGAGAGAGGAACGAAACATTCCTTGTAAACATGTGGAAACTTTTCTTTAGTAGATGCATTTTAAAATTGTGACGCGATACATAACGGGCCAAAACGAACAATATTTGCTAGCAGTGGGCTTGAATTGTTACAAATGGTATCAGAGCCAGATATTGGGCGGTGTGCGAGTGAGGACGTTGGGCCCTCAAGGGGGCTAGATTGTGAGATCCTACATCAGTTAGAGAGGGAGATAAAACATTTCTTCTAAACATGTGGAAACCTCTCCTTAGTATATGCGTTTTAAAATCGTGAGGCTGACGACGATACGTAACGGGCCAAAATGGACAATATCTGCTAGTAGTGGGCTTTAGCTGTTACAAATGGTATCGGAGTCAGATATCGAGCGGTGTGCCATGAGGACGTTGGGCCTTCAAGGGGGTGGATTGTGAGATTCCACATCGGTTGGAGAGGGGAACGAAACATTCCTTATAAGCGTGTAGAAACCTCTCTCTAGCAGATACGTCTTAAAACCGTGAGGCTAACGACAATATGTAACGGACCAAAACGGATAATATCTACTAACGGTAGATTCAGCCTGCTACATCTATCGAAAGGCCACGAGTGTTCTTCTAGGTTTTTAAGTTTACTTATAAATATATTTAAATTTGTTTACGTTAATCTATCCGCTTAAGCTTTTGATTTAGCAGTAGCGTTAAGAGTAAACTTAAGGGCATGCAATTGGCAGACAAGAGAGTGTGAGAATCAAGACCAAGCCAGGTTTTGCAAAGGTGATGGGAACAATTGTGTGTATATGTGGAGCAATGCTGCTATCTTTCTACCATGGACACACCATTGGCTTAGGTGAATCAAAAATTCACTGGCCTTATGTCGAGAGGATCATGAGCAAAACAAACCCGACGAACCGTCAAGAAAACCGAACCCTCGGCTCGGTTTTGTTGCTTTGCAGCTCGTTCTCTTGGGCGTTATGGTTCGTCATTCAAGTAAGTAGCCATAACCCCTAATTTGCTATATTTTTCAAGCCAAAGACAGCTGAAACTATTTTTTGCAGGCAAGATTGAGTGTCAAGTTTAAAGCTCCCTATACAAGTACAACGTTGTTGTGCTTCATGGCCTTTTTCCAATGTGGACTCATAGCTGTAATTTCCGAGCACAATGTCGCTGCTTGGTCATTAAACAGTCCGATCCGACTCATCTCAGCCCTTTACACGGTATGTAACGTACTTAATAATCGCTCGATGCACGGTGTCGATATGTCTGTATGAAACTAATGGAAGTTTTCACAGGGAATTGTGTGTTCTGGATTGACATTTAGCATCACTTCTTGGATTATACAAAGGAAAGGGCCTCTATATGTCTCAATTTTCACCCCATTGTTGCTTATCATTGTGGCCATCTTTAGCTGGACTCTCCTCCACGAACAGCTCTACATCGGAACGTGCGTATCCATGTCACTCTCGTATATTCAAACTTCTGACACGCTTTCTATTTCGTATAGAACTGTTTATACATAAACATAATCATACTCCACACACTCATATATTCGAATTTATGAGATCTTTTCTGTTTCGTATAGAACTGTTTACTTATTGGGTTTTGTGCGTTCGTGATGGTCCGTTTTTAACAGTGTTCTTGGCTCTATTTTGATAATCATTGGGCTATATGGAGTGCTATGGGGAAAGTCCAAAGAGATGAAAGTAGAAGAGGAAGACAAGGTGGAAAAGGCAACAAATGGGAGTCAAAAAGATGACGTGGAATTGCAAATCATAAACATCAAATAAAGGGAAAGAAA

mRNA sequence

ATGGAGGGAGATATCACAGCAATCATGGGGATGGTTATATTGCAATTTTGCTACGCTGGTTTAAGCATTACCTCAAAGCTAGCGATGCAATCAGGAATGAGCCCTCTTGTTCTCCTCACTTACAGGCAATTTTTTGGCACTTTGGCCATTGCTCCCTTTGCTTTCTTTACTGAAAGGAAAACAAGGCCAAAGATCACTTTCCCTGTTCTTGTTCAAATCTTTATCTGTTCTTTATCTGGAGCCACAGCGAACCAGATATTCTTCTTTGTTGGGTTGAAATGCACAAACCCAACCGTTTCAAGTGCTATGGCTAATGTTCTTCCTGCTGCTACTTTTATTCTTGCTGTTGTTTTCAGACAAGAGAGTGTGAGAATCAAGACCAAGCCAGGTTTTGCAAAGGTGATGGGAACAATTGTGTGTATATGTGGAGCAATGCTGCTATCTTTCTACCATGGACACACCATTGGCTTAGGTGAATCAAAAATTCACTGGCCTTATGTCGAGAGGATCATGAGCAAAACAAACCCGACGAACCGTCAAGAAAACCGAACCCTCGGCTCGGTTTTGTTGCTTTGCAGCTCGTTCTCTTGGGCGTTATGGTTCGTCATTCAAGCAAGATTGAGTGTCAAGTTTAAAGCTCCCTATACAAGTACAACGTTGTTGTGCTTCATGGCCTTTTTCCAATGTGGACTCATAGCTGTAATTTCCGAGCACAATGTCGCTGCTTGGTCATTAAACAGTCCGATCCGACTCATCTCAGCCCTTTACACGGGAATTGTGTGTTCTGGATTGACATTTAGCATCACTTCTTGGATTATACAAAGGAAAGGGCCTCTATATGTCTCAATTTTCACCCCATTGTTGCTTATCATTGTGGCCATCTTTAGCTGGACTCTCCTCCACGAACAGCTCTACATCGGAACTGTTCTTGGCTCTATTTTGATAATCATTGGGCTATATGGAGTGCTATGGGGAAAGTCCAAAGAGATGAAAGTAGAAGAGGAAGACAAGGTGGAAAAGGCAACAAATGGGAGTCAAAAAGATGACGTGGAATTGCAAATCATAAACATCAAATAAAGGGAAAGAAA

Coding sequence (CDS)

ATGGAGGGAGATATCACAGCAATCATGGGGATGGTTATATTGCAATTTTGCTACGCTGGTTTAAGCATTACCTCAAAGCTAGCGATGCAATCAGGAATGAGCCCTCTTGTTCTCCTCACTTACAGGCAATTTTTTGGCACTTTGGCCATTGCTCCCTTTGCTTTCTTTACTGAAAGGAAAACAAGGCCAAAGATCACTTTCCCTGTTCTTGTTCAAATCTTTATCTGTTCTTTATCTGGAGCCACAGCGAACCAGATATTCTTCTTTGTTGGGTTGAAATGCACAAACCCAACCGTTTCAAGTGCTATGGCTAATGTTCTTCCTGCTGCTACTTTTATTCTTGCTGTTGTTTTCAGACAAGAGAGTGTGAGAATCAAGACCAAGCCAGGTTTTGCAAAGGTGATGGGAACAATTGTGTGTATATGTGGAGCAATGCTGCTATCTTTCTACCATGGACACACCATTGGCTTAGGTGAATCAAAAATTCACTGGCCTTATGTCGAGAGGATCATGAGCAAAACAAACCCGACGAACCGTCAAGAAAACCGAACCCTCGGCTCGGTTTTGTTGCTTTGCAGCTCGTTCTCTTGGGCGTTATGGTTCGTCATTCAAGCAAGATTGAGTGTCAAGTTTAAAGCTCCCTATACAAGTACAACGTTGTTGTGCTTCATGGCCTTTTTCCAATGTGGACTCATAGCTGTAATTTCCGAGCACAATGTCGCTGCTTGGTCATTAAACAGTCCGATCCGACTCATCTCAGCCCTTTACACGGGAATTGTGTGTTCTGGATTGACATTTAGCATCACTTCTTGGATTATACAAAGGAAAGGGCCTCTATATGTCTCAATTTTCACCCCATTGTTGCTTATCATTGTGGCCATCTTTAGCTGGACTCTCCTCCACGAACAGCTCTACATCGGAACTGTTCTTGGCTCTATTTTGATAATCATTGGGCTATATGGAGTGCTATGGGGAAAGTCCAAAGAGATGAAAGTAGAAGAGGAAGACAAGGTGGAAAAGGCAACAAATGGGAGTCAAAAAGATGACGTGGAATTGCAAATCATAAACATCAAATAA

Protein sequence

MEGDITAIMGMVILQFCYAGLSITSKLAMQSGMSPLVLLTYRQFFGTLAIAPFAFFTERKTRPKITFPVLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQESVRIKTKPGFAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVAAWSLNSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWTLLHEQLYIGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVELQIINIK
Homology
BLAST of CmaCh05G010620 vs. ExPASy Swiss-Prot
Match: Q8GXB4 (WAT1-related protein At1g09380 OS=Arabidopsis thaliana OX=3702 GN=At1g09380 PE=2 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 2.4e-113
Identity = 198/358 (55.31%), Postives = 270/358 (75.42%), Query Frame = 0

Query: 2   EGDITAIMGMVILQFCYAGLSITSKLAMQSGMSPLVLLTYRQFFGTLAIAPFAFFTERKT 61
           + D+   + MV++Q  YAG++ITSK+AM++GM PL+L+ YRQ F T+A  P AFF ERKT
Sbjct: 3   KSDMLPFLAMVLVQIGYAGMNITSKMAMEAGMKPLILVAYRQIFATIATFPVAFFLERKT 62

Query: 62  RPKITFPVLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQE 121
           RPKIT  +LVQ+F CS++GAT NQ+ +FVGL+ ++PT++ A+ N+LPA TF+LA +FRQE
Sbjct: 63  RPKITLRILVQVFFCSITGATGNQVLYFVGLQNSSPTIACALTNLLPAVTFLLAAIFRQE 122

Query: 122 SVRIKTKPGFAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQE 181
           +V IK   G AKV+GT+VC+ GAM+LSFYHGHTIG+GESKIHW Y E I +K   ++   
Sbjct: 123 TVGIKKASGQAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAYAENI-TKHGSSSGHS 182

Query: 182 NRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVA 241
           N  LG  L++ ++ SWA WF+IQ ++S  F APYTST L+C M   QCG IA+IS+H ++
Sbjct: 183 NFFLGPFLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLLMCLMGSIQCGAIALISDHTIS 242

Query: 242 AWSLNSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWTLLH 301
            WSL+SP+R ISALY G+V S L F + SW +QRKGPLYVS+F+PLLL++VAIFSW LL 
Sbjct: 243 DWSLSSPLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLLLVVVAIFSWALLE 302

Query: 302 EQLYIGTVLGSILIIIGLYGVLWGKSKEM--KVEEEDKVEKATN---GSQKDDVELQI 355
           E+LY GT +GS L++IGLYGVLWGK +E+  K EE +KV++  +       +D+E ++
Sbjct: 303 EKLYTGTFMGSALVVIGLYGVLWGKDREVSEKEEEREKVKQQNHKVKSESNEDIESRL 359

BLAST of CmaCh05G010620 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 5.7e-67
Identity = 138/344 (40.12%), Postives = 221/344 (64.24%), Query Frame = 0

Query: 11  MVILQFCYAGLSITSKLAMQSGMSPLVLLTYRQFFGTLAIAPFAFFTERKTRPKITFPVL 70
           M+ LQF YAG++I +K+++ +GMS  VL+ YR    T  IAPFAFF ERK +PKITF + 
Sbjct: 22  MISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKITFSIF 81

Query: 71  VQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQESVRIKTKPG 130
           +Q+FI  L G   +Q F+++GLK T+PT S AM+N+LPA TFILAV+FR E + +K    
Sbjct: 82  MQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKLWC 141

Query: 131 FAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQENRTL--GSV 190
            AK+ GT+V + GAML++ Y G  + L  +K          + T+  N   ++    GS+
Sbjct: 142 QAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFLKGSI 201

Query: 191 LLLCSSFSWALWFVIQAR-LSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVAAWSLNS 250
           LL+ ++ +WA  FV+QA+ L    K   + TTL+CF+   Q   +  + EHN +AW +  
Sbjct: 202 LLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAWRIGW 261

Query: 251 PIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWTLLHEQLYIG 310
            + L++A Y+GIV S +++ +   +++++GP++ + F+PL+++IVA+    +L E++++G
Sbjct: 262 DMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIFLG 321

Query: 311 TVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVE 352
            V+G++LI+IGLY VLWGK KE +V   +  +  +N    +DVE
Sbjct: 322 GVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTEDVE 365

BLAST of CmaCh05G010620 vs. ExPASy Swiss-Prot
Match: Q9LI65 (WAT1-related protein At3g30340 OS=Arabidopsis thaliana OX=3702 GN=At3g30340 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.6e-64
Identity = 127/324 (39.20%), Postives = 212/324 (65.43%), Query Frame = 0

Query: 7   AIMGMVILQFCYAGLSITSKLAMQSGMSPLVLLTYRQFFGTLAIAPFAFFTERKTRPKIT 66
           A++ M ++    + +++  K  +  G++ +V  TYR   GTL + PFA F ER  RPK+T
Sbjct: 11  AVLMMSMINIGLSVVNVMFKKMIDEGLNRMVATTYRLAVGTLFLIPFAIFLERHNRPKLT 70

Query: 67  FPVLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQESVRIK 126
             +L  +F  +L G +  Q FF +GL+ T+ T S A +N++P+ TF LA+VFRQE++ IK
Sbjct: 71  GRILCSLFFSALLGTSLVQYFFLIGLEYTSSTFSLAFSNMVPSVTFALALVFRQETLNIK 130

Query: 127 TKPGFAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQENR-TL 186
           +  G AK++GT++CICGA++L+ Y G  +    S+ H  ++E   ++T+ T     +  +
Sbjct: 131 SNVGRAKLLGTMICICGALVLTLYKGTAL----SREHSTHME-THTRTDSTGAMTQKWAM 190

Query: 187 GSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVAAWSL 246
           GS++L+ S   W+ WF++QA++S  +   YTSTT+L F    Q  L+++ISE + + W +
Sbjct: 191 GSIMLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTSMWVV 250

Query: 247 NSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWTLLHEQLY 306
               ++++ LY+GIV SGL +   SW ++++G ++ S F PL+ +  AIFS++ LHEQ+Y
Sbjct: 251 KDKFQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLHEQIY 310

Query: 307 IGTVLGSILIIIGLYGVLWGKSKE 330
            G+V+GS++II+GLY +LWGKSK+
Sbjct: 311 CGSVIGSMVIIVGLYILLWGKSKD 329

BLAST of CmaCh05G010620 vs. ExPASy Swiss-Prot
Match: F4IJ08 (WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 7.2e-62
Identity = 130/331 (39.27%), Postives = 208/331 (62.84%), Query Frame = 0

Query: 11  MVILQFCYAGLSITSKLAMQSGMSPLVLLTYRQFFGTLAIAPFAFFTERKTRPKITFPVL 70
           MV LQF YAG+++ +K  +  GMS  VL+ YR  F T AIAPFA  +ERK R K+TFP+ 
Sbjct: 15  MVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVRSKMTFPIF 74

Query: 71  VQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQESVRIKTKPG 130
           ++IF+ +L G   +Q  +++GLK T+PT SSA++N++PA T ILA +FR E V ++    
Sbjct: 75  MRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEKVEMRKVRC 134

Query: 131 FAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQENRTLGSVLL 190
             KVMGT+V + G++L+ FY G  I    S +         + + PT    +    +V L
Sbjct: 135 LVKVMGTLVTVVGSILMIFYKGPFINFFRSHL-------TAASSPPT---ADYLKAAVFL 194

Query: 191 LCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVAAWSLNSPIR 250
           L +S SWA +FV+QA    K+ A  + +T++CFM   Q   +A + EHN +A ++   + 
Sbjct: 195 LLASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHNPSALNIGFDMN 254

Query: 251 LISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWTLLHEQLYIGTVL 310
           L+++ Y GI+ S + + +   ++QRKGP++V+ F PL+++IV+I S+ +L + +Y+G V+
Sbjct: 255 LLASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFVLGQGIYLGGVI 314

Query: 311 GSILIIIGLYGVLWGKSKEMKVEE---EDKV 339
           G +++++G+Y VLWGK  +   EE   ED V
Sbjct: 315 GVVVLMVGVYAVLWGKHVDDDGEETRHEDNV 335

BLAST of CmaCh05G010620 vs. ExPASy Swiss-Prot
Match: Q94AP3 (Protein WALLS ARE THIN 1 OS=Arabidopsis thaliana OX=3702 GN=WAT1 PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 8.0e-61
Identity = 128/331 (38.67%), Postives = 193/331 (58.31%), Query Frame = 0

Query: 9   MGMVILQFCYAGLSITSKLAMQSGMSPLVLLTYRQFFGTLAIAPFAFFTERKTRPKITFP 68
           + M+ LQF YAG  + S+ A+  G+S LV   YR     L + PFA+F E+K RP IT  
Sbjct: 22  IAMLTLQFGYAGFHVVSRAALNMGISKLVFPVYRNIIALLLLLPFAYFLEKKERPAITLN 81

Query: 69  VLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQESVRIKTK 128
            L+Q F  +L G TANQ F+ +GL  T+PT +S+M N +PA TF++A + R E VRI  +
Sbjct: 82  FLIQFFFLALIGITANQGFYLLGLDNTSPTFASSMQNSVPAITFLMAALLRIEKVRINRR 141

Query: 129 PGFAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNR--QENRTLG 188
            G +K++GT +C+ GA +++ Y G TI    S +H   +    +   P      +N TLG
Sbjct: 142 DGISKILGTALCVAGASVITLYKGPTIYTPASHLHAHLLTTNSAVLAPLGNAAPKNWTLG 201

Query: 189 SVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVAAWSLN 248
            + L+    SW+ W V QA +   + A  + T+  CF    Q  +IA   E +  AW  +
Sbjct: 202 CIYLIGHCLSWSGWLVFQAPVLKSYPARLSVTSYTCFFGIIQFLIIAAFCERDSQAWVFH 261

Query: 249 SPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWTLLHEQLYI 308
           S   L + LY GIV SG+ F++  W I R GP++V+++ P+  ++VAI +   L E+ Y+
Sbjct: 262 SGWELFTILYAGIVASGIAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIMASIALGEEFYL 321

Query: 309 GTVLGSILIIIGLYGVLWGKSKEMKVEEEDK 338
           G ++G++LII GLY VL+GKS+E K    +K
Sbjct: 322 GGIIGAVLIIAGLYFVLYGKSEERKFAALEK 352

BLAST of CmaCh05G010620 vs. TAIR 10
Match: AT1G09380.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 410.2 bits (1053), Expect = 1.7e-114
Identity = 198/358 (55.31%), Postives = 270/358 (75.42%), Query Frame = 0

Query: 2   EGDITAIMGMVILQFCYAGLSITSKLAMQSGMSPLVLLTYRQFFGTLAIAPFAFFTERKT 61
           + D+   + MV++Q  YAG++ITSK+AM++GM PL+L+ YRQ F T+A  P AFF ERKT
Sbjct: 3   KSDMLPFLAMVLVQIGYAGMNITSKMAMEAGMKPLILVAYRQIFATIATFPVAFFLERKT 62

Query: 62  RPKITFPVLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQE 121
           RPKIT  +LVQ+F CS++GAT NQ+ +FVGL+ ++PT++ A+ N+LPA TF+LA +FRQE
Sbjct: 63  RPKITLRILVQVFFCSITGATGNQVLYFVGLQNSSPTIACALTNLLPAVTFLLAAIFRQE 122

Query: 122 SVRIKTKPGFAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQE 181
           +V IK   G AKV+GT+VC+ GAM+LSFYHGHTIG+GESKIHW Y E I +K   ++   
Sbjct: 123 TVGIKKASGQAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAYAENI-TKHGSSSGHS 182

Query: 182 NRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVA 241
           N  LG  L++ ++ SWA WF+IQ ++S  F APYTST L+C M   QCG IA+IS+H ++
Sbjct: 183 NFFLGPFLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLLMCLMGSIQCGAIALISDHTIS 242

Query: 242 AWSLNSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWTLLH 301
            WSL+SP+R ISALY G+V S L F + SW +QRKGPLYVS+F+PLLL++VAIFSW LL 
Sbjct: 243 DWSLSSPLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLLLVVVAIFSWALLE 302

Query: 302 EQLYIGTVLGSILIIIGLYGVLWGKSKEM--KVEEEDKVEKATN---GSQKDDVELQI 355
           E+LY GT +GS L++IGLYGVLWGK +E+  K EE +KV++  +       +D+E ++
Sbjct: 303 EKLYTGTFMGSALVVIGLYGVLWGKDREVSEKEEEREKVKQQNHKVKSESNEDIESRL 359

BLAST of CmaCh05G010620 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 256.1 bits (653), Expect = 4.1e-68
Identity = 138/344 (40.12%), Postives = 221/344 (64.24%), Query Frame = 0

Query: 11  MVILQFCYAGLSITSKLAMQSGMSPLVLLTYRQFFGTLAIAPFAFFTERKTRPKITFPVL 70
           M+ LQF YAG++I +K+++ +GMS  VL+ YR    T  IAPFAFF ERK +PKITF + 
Sbjct: 22  MISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKITFSIF 81

Query: 71  VQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQESVRIKTKPG 130
           +Q+FI  L G   +Q F+++GLK T+PT S AM+N+LPA TFILAV+FR E + +K    
Sbjct: 82  MQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKLWC 141

Query: 131 FAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQENRTL--GSV 190
            AK+ GT+V + GAML++ Y G  + L  +K          + T+  N   ++    GS+
Sbjct: 142 QAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFLKGSI 201

Query: 191 LLLCSSFSWALWFVIQAR-LSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVAAWSLNS 250
           LL+ ++ +WA  FV+QA+ L    K   + TTL+CF+   Q   +  + EHN +AW +  
Sbjct: 202 LLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAWRIGW 261

Query: 251 PIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWTLLHEQLYIG 310
            + L++A Y+GIV S +++ +   +++++GP++ + F+PL+++IVA+    +L E++++G
Sbjct: 262 DMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIFLG 321

Query: 311 TVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVE 352
            V+G++LI+IGLY VLWGK KE +V   +  +  +N    +DVE
Sbjct: 322 GVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTEDVE 365

BLAST of CmaCh05G010620 vs. TAIR 10
Match: AT3G30340.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 248.1 bits (632), Expect = 1.1e-65
Identity = 127/324 (39.20%), Postives = 212/324 (65.43%), Query Frame = 0

Query: 7   AIMGMVILQFCYAGLSITSKLAMQSGMSPLVLLTYRQFFGTLAIAPFAFFTERKTRPKIT 66
           A++ M ++    + +++  K  +  G++ +V  TYR   GTL + PFA F ER  RPK+T
Sbjct: 11  AVLMMSMINIGLSVVNVMFKKMIDEGLNRMVATTYRLAVGTLFLIPFAIFLERHNRPKLT 70

Query: 67  FPVLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQESVRIK 126
             +L  +F  +L G +  Q FF +GL+ T+ T S A +N++P+ TF LA+VFRQE++ IK
Sbjct: 71  GRILCSLFFSALLGTSLVQYFFLIGLEYTSSTFSLAFSNMVPSVTFALALVFRQETLNIK 130

Query: 127 TKPGFAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQENR-TL 186
           +  G AK++GT++CICGA++L+ Y G  +    S+ H  ++E   ++T+ T     +  +
Sbjct: 131 SNVGRAKLLGTMICICGALVLTLYKGTAL----SREHSTHME-THTRTDSTGAMTQKWAM 190

Query: 187 GSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVAAWSL 246
           GS++L+ S   W+ WF++QA++S  +   YTSTT+L F    Q  L+++ISE + + W +
Sbjct: 191 GSIMLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTSMWVV 250

Query: 247 NSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWTLLHEQLY 306
               ++++ LY+GIV SGL +   SW ++++G ++ S F PL+ +  AIFS++ LHEQ+Y
Sbjct: 251 KDKFQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLHEQIY 310

Query: 307 IGTVLGSILIIIGLYGVLWGKSKE 330
            G+V+GS++II+GLY +LWGKSK+
Sbjct: 311 CGSVIGSMVIIVGLYILLWGKSKD 329

BLAST of CmaCh05G010620 vs. TAIR 10
Match: AT2G40900.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 239.2 bits (609), Expect = 5.1e-63
Identity = 130/331 (39.27%), Postives = 208/331 (62.84%), Query Frame = 0

Query: 11  MVILQFCYAGLSITSKLAMQSGMSPLVLLTYRQFFGTLAIAPFAFFTERKTRPKITFPVL 70
           MV LQF YAG+++ +K  +  GMS  VL+ YR  F T AIAPFA  +ERK R K+TFP+ 
Sbjct: 15  MVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVRSKMTFPIF 74

Query: 71  VQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQESVRIKTKPG 130
           ++IF+ +L G   +Q  +++GLK T+PT SSA++N++PA T ILA +FR E V ++    
Sbjct: 75  MRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEKVEMRKVRC 134

Query: 131 FAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQENRTLGSVLL 190
             KVMGT+V + G++L+ FY G  I    S +         + + PT    +    +V L
Sbjct: 135 LVKVMGTLVTVVGSILMIFYKGPFINFFRSHL-------TAASSPPT---ADYLKAAVFL 194

Query: 191 LCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVAAWSLNSPIR 250
           L +S SWA +FV+QA    K+ A  + +T++CFM   Q   +A + EHN +A ++   + 
Sbjct: 195 LLASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHNPSALNIGFDMN 254

Query: 251 LISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWTLLHEQLYIGTVL 310
           L+++ Y GI+ S + + +   ++QRKGP++V+ F PL+++IV+I S+ +L + +Y+G V+
Sbjct: 255 LLASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFVLGQGIYLGGVI 314

Query: 311 GSILIIIGLYGVLWGKSKEMKVEE---EDKV 339
           G +++++G+Y VLWGK  +   EE   ED V
Sbjct: 315 GVVVLMVGVYAVLWGKHVDDDGEETRHEDNV 335

BLAST of CmaCh05G010620 vs. TAIR 10
Match: AT1G75500.1 (Walls Are Thin 1 )

HSP 1 Score: 235.7 bits (600), Expect = 5.7e-62
Identity = 128/331 (38.67%), Postives = 193/331 (58.31%), Query Frame = 0

Query: 9   MGMVILQFCYAGLSITSKLAMQSGMSPLVLLTYRQFFGTLAIAPFAFFTERKTRPKITFP 68
           + M+ LQF YAG  + S+ A+  G+S LV   YR     L + PFA+F E+K RP IT  
Sbjct: 22  IAMLTLQFGYAGFHVVSRAALNMGISKLVFPVYRNIIALLLLLPFAYFLEKKERPAITLN 81

Query: 69  VLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQESVRIKTK 128
            L+Q F  +L G TANQ F+ +GL  T+PT +S+M N +PA TF++A + R E VRI  +
Sbjct: 82  FLIQFFFLALIGITANQGFYLLGLDNTSPTFASSMQNSVPAITFLMAALLRIEKVRINRR 141

Query: 129 PGFAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNR--QENRTLG 188
            G +K++GT +C+ GA +++ Y G TI    S +H   +    +   P      +N TLG
Sbjct: 142 DGISKILGTALCVAGASVITLYKGPTIYTPASHLHAHLLTTNSAVLAPLGNAAPKNWTLG 201

Query: 189 SVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVAAWSLN 248
            + L+    SW+ W V QA +   + A  + T+  CF    Q  +IA   E +  AW  +
Sbjct: 202 CIYLIGHCLSWSGWLVFQAPVLKSYPARLSVTSYTCFFGIIQFLIIAAFCERDSQAWVFH 261

Query: 249 SPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWTLLHEQLYI 308
           S   L + LY GIV SG+ F++  W I R GP++V+++ P+  ++VAI +   L E+ Y+
Sbjct: 262 SGWELFTILYAGIVASGIAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIMASIALGEEFYL 321

Query: 309 GTVLGSILIIIGLYGVLWGKSKEMKVEEEDK 338
           G ++G++LII GLY VL+GKS+E K    +K
Sbjct: 322 GGIIGAVLIIAGLYFVLYGKSEERKFAALEK 352

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GXB42.4e-11355.31WAT1-related protein At1g09380 OS=Arabidopsis thaliana OX=3702 GN=At1g09380 PE=2... [more]
Q9FL415.7e-6740.12WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2... [more]
Q9LI651.6e-6439.20WAT1-related protein At3g30340 OS=Arabidopsis thaliana OX=3702 GN=At3g30340 PE=2... [more]
F4IJ087.2e-6239.27WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2... [more]
Q94AP38.0e-6138.67Protein WALLS ARE THIN 1 OS=Arabidopsis thaliana OX=3702 GN=WAT1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
AT1G09380.11.7e-11455.31nodulin MtN21 /EamA-like transporter family protein [more]
AT5G07050.14.1e-6840.12nodulin MtN21 /EamA-like transporter family protein [more]
AT3G30340.11.1e-6539.20nodulin MtN21 /EamA-like transporter family protein [more]
AT2G40900.15.1e-6339.27nodulin MtN21 /EamA-like transporter family protein [more]
AT1G75500.15.7e-6238.67Walls Are Thin 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 7..148
e-value: 1.5E-13
score: 51.1
coord: 185..323
e-value: 4.5E-18
score: 65.7
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 7..353
NoneNo IPR availablePANTHERPTHR31218:SF344WAT1-RELATED PROTEINcoord: 7..353
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 247..328
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 41..151

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G010620.1CmaCh05G010620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity