CmaCh04G015250 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G015250
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionAspartyl protease family protein
LocationCma_Chr04: 7726201 .. 7727655 (+)
RNA-Seq ExpressionCmaCh04G015250
SyntenyCmaCh04G015250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACCTTTCTTCTCTTCCTTCTCCCCCTTCTTTACGCCTCTTTTTCCGCCGTTCTCTCCCGGACCTTGCCTCACGCCCCTCAAACCTCCGTGCTCGATGTCGCTGCTTCCATTCACACCACTCGGCAGGTCTTTGCATTTCAGCCTCAATCTCCAATGCGAGATGAAAGTACCGTCTCGGACTCATCTTCCTTGACTCTCTTGTTGAATTCCAGGGTTTCCATTATGAAAACCTCGCACACTGACTACAAATCCCTCACATTATCCAGACTCTACCGCGACTCTGCTCGAGTCAGATCTTTGACTGCAAGGATGGATCTAGCCATTCCAGGTATTACTGGAGCAGATCTTGAACCACTCCCGACTGGTGGTGCTTCGCAATTTGGGATGGAGGATTTCGAGAGTCCGATTGTCTCAGGCGCGAGTCAGGGGAGCGGTGAGTACTTCTCCCGAGTTGGAATCGGTAGGCCGCCGAGTCCGGTTTACATGGTGCTCGATACTGGTAGTGATGTAAGTTGGGTACAATGCGCGCCTTGCGCTGATTGCTACGAGCAAACCGATCCAATTTTTGAGCCTACTTCCTCTGCTTCTTTCACGTCTCTGTCCTGCCAAACACAGCAATGTAAATCGCTCGATGTTTCTGAGTGCCGGAATGGTACTTGTCTCTACGAGGTTTCTTACGGCGATGGTTCTTACACCGTCGGCGATTTCGTTACTGAAACTGTTACTCTCGGCTCGACTTCTCTCACCAACATTGCTCTAGGCTGTGGCCATAACAATGAGGGTTTGTTCATCGGCGCCGCCGGTTTGCTCGGACTAGGAGGCGGCTCGCTCTCGTTCCCTTCGCAGCTTAATGCCTCGTCTTTTTCGTACTGTCTCGTGGACCGTGACTCTGAATCCACCTCGACTCTCGATTTCAACTCACCGATTCCTCCCGATGCCGTAACCGCGCCGCTGCACCGAAACCCTAATTTGGACACTTTTTTCTACCTCGGCATGACAGGGATGAGCGTCGGAGGTGAAATTCTTCCGATTCCCGAGACGTCGTTCCAAATGAGCCAAGACGGAAACGGCGGCATCATCATTGACTCCGGCACCGCCGTGACGCGGTTGCAGACCACCGCTTATAACTTGTTGCGCGACGCGTTCGTTAAGAAGACGCACGATTTGCAGTCCGCACGTGGCGTGGCGTTGTTCGATACTTGTTACGACTTGTCGTCGAAGTCGAGAGTCGAAGTGCCGACGGTGTCATTTCACTTTGCGGACGGGAAAGAGTTGCCGTTGCCGGCCAAAAATTACCTGATACCGGTTGACTCGGAAGGAACATTTTGTTTCGCCTTTGCCCCTACCGATTCAACGTTGTCAATACTCGGGAACGCACAGCAGCAAGGGACACGTGTCAGTTTCGACCTCGCTAATTCGCTCGTTGGGTTCTCGTCCAACAAATGCTAA

mRNA sequence

ATGGCCACCTTTCTTCTCTTCCTTCTCCCCCTTCTTTACGCCTCTTTTTCCGCCGTTCTCTCCCGGACCTTGCCTCACGCCCCTCAAACCTCCGTGCTCGATGTCGCTGCTTCCATTCACACCACTCGGCAGGTCTTTGCATTTCAGCCTCAATCTCCAATGCGAGATGAAAGTACCGTCTCGGACTCATCTTCCTTGACTCTCTTGTTGAATTCCAGGGTTTCCATTATGAAAACCTCGCACACTGACTACAAATCCCTCACATTATCCAGACTCTACCGCGACTCTGCTCGAGTCAGATCTTTGACTGCAAGGATGGATCTAGCCATTCCAGGTATTACTGGAGCAGATCTTGAACCACTCCCGACTGGTGGTGCTTCGCAATTTGGGATGGAGGATTTCGAGAGTCCGATTGTCTCAGGCGCGAGTCAGGGGAGCGGTGAGTACTTCTCCCGAGTTGGAATCGGTAGGCCGCCGAGTCCGGTTTACATGGTGCTCGATACTGGTAGTGATGTAAGTTGGGTACAATGCGCGCCTTGCGCTGATTGCTACGAGCAAACCGATCCAATTTTTGAGCCTACTTCCTCTGCTTCTTTCACGTCTCTGTCCTGCCAAACACAGCAATGTAAATCGCTCGATGTTTCTGAGTGCCGGAATGGTACTTGTCTCTACGAGGTTTCTTACGGCGATGGTTCTTACACCGTCGGCGATTTCGTTACTGAAACTGTTACTCTCGGCTCGACTTCTCTCACCAACATTGCTCTAGGCTGTGGCCATAACAATGAGGGTTTGTTCATCGGCGCCGCCGGTTTGCTCGGACTAGGAGGCGGCTCGCTCTCGTTCCCTTCGCAGCTTAATGCCTCGTCTTTTTCGTACTGTCTCGTGGACCGTGACTCTGAATCCACCTCGACTCTCGATTTCAACTCACCGATTCCTCCCGATGCCGTAACCGCGCCGCTGCACCGAAACCCTAATTTGGACACTTTTTTCTACCTCGGCATGACAGGGATGAGCGTCGGAGGTGAAATTCTTCCGATTCCCGAGACGTCGTTCCAAATGAGCCAAGACGGAAACGGCGGCATCATCATTGACTCCGGCACCGCCGTGACGCGGTTGCAGACCACCGCTTATAACTTGTTGCGCGACGCGTTCGTTAAGAAGACGCACGATTTGCAGTCCGCACGTGGCGTGGCGTTGTTCGATACTTGTTACGACTTGTCGTCGAAGTCGAGAGTCGAAGTGCCGACGGTGTCATTTCACTTTGCGGACGGGAAAGAGTTGCCGTTGCCGGCCAAAAATTACCTGATACCGGTTGACTCGGAAGGAACATTTTGTTTCGCCTTTGCCCCTACCGATTCAACGTTGTCAATACTCGGGAACGCACAGCAGCAAGGGACACGTGTCAGTTTCGACCTCGCTAATTCGCTCGTTGGGTTCTCGTCCAACAAATGCTAA

Coding sequence (CDS)

ATGGCCACCTTTCTTCTCTTCCTTCTCCCCCTTCTTTACGCCTCTTTTTCCGCCGTTCTCTCCCGGACCTTGCCTCACGCCCCTCAAACCTCCGTGCTCGATGTCGCTGCTTCCATTCACACCACTCGGCAGGTCTTTGCATTTCAGCCTCAATCTCCAATGCGAGATGAAAGTACCGTCTCGGACTCATCTTCCTTGACTCTCTTGTTGAATTCCAGGGTTTCCATTATGAAAACCTCGCACACTGACTACAAATCCCTCACATTATCCAGACTCTACCGCGACTCTGCTCGAGTCAGATCTTTGACTGCAAGGATGGATCTAGCCATTCCAGGTATTACTGGAGCAGATCTTGAACCACTCCCGACTGGTGGTGCTTCGCAATTTGGGATGGAGGATTTCGAGAGTCCGATTGTCTCAGGCGCGAGTCAGGGGAGCGGTGAGTACTTCTCCCGAGTTGGAATCGGTAGGCCGCCGAGTCCGGTTTACATGGTGCTCGATACTGGTAGTGATGTAAGTTGGGTACAATGCGCGCCTTGCGCTGATTGCTACGAGCAAACCGATCCAATTTTTGAGCCTACTTCCTCTGCTTCTTTCACGTCTCTGTCCTGCCAAACACAGCAATGTAAATCGCTCGATGTTTCTGAGTGCCGGAATGGTACTTGTCTCTACGAGGTTTCTTACGGCGATGGTTCTTACACCGTCGGCGATTTCGTTACTGAAACTGTTACTCTCGGCTCGACTTCTCTCACCAACATTGCTCTAGGCTGTGGCCATAACAATGAGGGTTTGTTCATCGGCGCCGCCGGTTTGCTCGGACTAGGAGGCGGCTCGCTCTCGTTCCCTTCGCAGCTTAATGCCTCGTCTTTTTCGTACTGTCTCGTGGACCGTGACTCTGAATCCACCTCGACTCTCGATTTCAACTCACCGATTCCTCCCGATGCCGTAACCGCGCCGCTGCACCGAAACCCTAATTTGGACACTTTTTTCTACCTCGGCATGACAGGGATGAGCGTCGGAGGTGAAATTCTTCCGATTCCCGAGACGTCGTTCCAAATGAGCCAAGACGGAAACGGCGGCATCATCATTGACTCCGGCACCGCCGTGACGCGGTTGCAGACCACCGCTTATAACTTGTTGCGCGACGCGTTCGTTAAGAAGACGCACGATTTGCAGTCCGCACGTGGCGTGGCGTTGTTCGATACTTGTTACGACTTGTCGTCGAAGTCGAGAGTCGAAGTGCCGACGGTGTCATTTCACTTTGCGGACGGGAAAGAGTTGCCGTTGCCGGCCAAAAATTACCTGATACCGGTTGACTCGGAAGGAACATTTTGTTTCGCCTTTGCCCCTACCGATTCAACGTTGTCAATACTCGGGAACGCACAGCAGCAAGGGACACGTGTCAGTTTCGACCTCGCTAATTCGCTCGTTGGGTTCTCGTCCAACAAATGCTAA

Protein sequence

MATFLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDESTVSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEPLPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGGIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFHFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFSSNKC
Homology
[Close]
BLAST of CmaCh04G015250 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 2.5e-150
Identity = 266/485 (54.85%), Postives = 355/485 (73.20%), Query Frame = 0 

Query: 12  LYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQP---------QSPMRDESTVSD 71
           L+ + +   SR+L   P+T+VLDV +S+  T+ + +  P            + D    + 
Sbjct: 17  LFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPESLSDPVFFNS 76

Query: 72  SSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEPLP 131
           SS L+L L+SR + + + H DYKSLTLSRL RDS+RV  + A++  A+ G+  +DL+P+ 
Sbjct: 77  SSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDLKPV- 136

Query: 132 TGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCAD 191
               +++  ED  +P+VSGASQGSGEYFSR+G+G P   +Y+VLDTGSDV+W+QC PCAD
Sbjct: 137 YNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCAD 196

Query: 192 CYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTET 251
           CY+Q+DP+F PTSS+++ SL+C   QC  L+ S CR+  CLY+VSYGDGS+TVG+  T+T
Sbjct: 197 CYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDT 256

Query: 252 VTLG-STSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSES 311
           VT G S  + N+ALGCGH+NEGLF GAAGLLGLGGG LS  +Q+ A+SFSYCLVDRDS  
Sbjct: 257 VTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGK 316

Query: 312 TSTLDFNS-PIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGG 371
           +S+LDFNS  +     TAPL RN  +DTF+Y+G++G SVGGE + +P+  F +   G+GG
Sbjct: 317 SSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGG 376

Query: 372 IIIDSGTAVTRLQTTAYNLLRDAFVKKTHDL-QSARGVALFDTCYDLSSKSRVEVPTVSF 431
           +I+D GTAVTRLQT AYN LRDAF+K T +L + +  ++LFDTCYD SS S V+VPTV+F
Sbjct: 377 VILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAF 436

Query: 432 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 485
           HF  GK L LPAKNYLIPVD  GTFCFAFAPT S+LSI+GN QQQGTR+++DL+ +++G 
Sbjct: 437 HFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGL 496

[Close]
BLAST of CmaCh04G015250 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 3.0e-111
Identity = 219/485 (45.15%), Postives = 299/485 (61.65%), Query Frame = 0 

Query: 4   FLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDESTVSDS 63
           F  FL   L+ S S+ +S      P   ++DV     T          +   DES    S
Sbjct: 6   FFFFLHLHLHLSSSSSIS-----FPDFQIIDVLQPPLTVTATLPDFNNTHFSDES----S 65

Query: 64  SSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEPLPT 123
           S  TL L  R      ++ ++     +R+ RD+ RV ++  R+   +  I  +D      
Sbjct: 66  SKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKV--IPSSD------ 125

Query: 124 GGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADC 183
              S++ + DF S IVSG  QGSGEYF R+G+G PP   YMV+D+GSD+ WVQC PC  C
Sbjct: 126 ---SRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLC 185

Query: 184 YEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETV 243
           Y+Q+DP+F+P  S S+T +SC +  C  ++ S C +G C YEV YGDGSYT G    ET+
Sbjct: 186 YKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETL 245

Query: 244 TLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLN---ASSFSYCLVDRDSE 303
           T   T + N+A+GCGH N G+FIGAAGLLG+GGGS+SF  QL+     +F YCLV R ++
Sbjct: 246 TFAKTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTD 305

Query: 304 STSTLDF-NSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 363
           ST +L F    +P  A   PL RNP   +F+Y+G+ G+ VGG  +P+P+  F +++ G+G
Sbjct: 306 STGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDG 365

Query: 364 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 423
           G+++D+GTAVTRL T AY   RD F  +T +L  A GV++FDTCYDLS    V VPTVSF
Sbjct: 366 GVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSF 425

Query: 424 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 483
           +F +G  L LPA+N+L+PVD  GT+CFAFA + + LSI+GN QQ+G +VSFD AN  VGF
Sbjct: 426 YFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGF 470

Query: 484 SSNKC 485
             N C
Sbjct: 486 GPNVC 470

[Close]
BLAST of CmaCh04G015250 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 9.6e-110
Identity = 227/444 (51.13%), Postives = 288/444 (64.86%), Query Frame = 0 

Query: 51  QSPMRDESTVSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAI 110
           +S     S    SSS+TL L+   ++      D   L  SRL RDS RV+S+ A +   I
Sbjct: 57  ESEFESGSDSESSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSI-ATLAAQI 116

Query: 111 PG--ITGADLEPLPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDT 170
           PG  +T A   P P G         F S +VSG SQGSGEYF+R+G+G P   VYMVLDT
Sbjct: 117 PGRNVTHA---PRPGG---------FSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDT 176

Query: 171 GSDVSWVQCAPCADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSEC--RNGTCLYEV 230
           GSD+ W+QCAPC  CY Q+DPIF+P  S ++ ++ C +  C+ LD + C  R  TCLY+V
Sbjct: 177 GSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQV 236

Query: 231 SYGDGSYTVGDFVTETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLN 290
           SYGDGS+TVGDF TET+T     +  +ALGCGH+NEGLF+GAAGLLGLG G LSFP Q  
Sbjct: 237 SYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTG 296

Query: 291 ---ASSFSYCLVDRDSES--TSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGG 350
                 FSYCLVDR + S  +S +  N+ +   A   PL  NP LDTF+Y+G+ G+SVGG
Sbjct: 297 HRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGG 356

Query: 351 EILP-IPETSFQMSQDGNGGIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALF 410
             +P +  + F++ Q GNGG+IIDSGT+VTRL   AY  +RDAF      L+ A   +LF
Sbjct: 357 TRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLF 416

Query: 411 DTCYDLSSKSRVEVPTVSFHFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGN 470
           DTC+DLS+ + V+VPTV  HF  G ++ LPA NYLIPVD+ G FCFAFA T   LSI+GN
Sbjct: 417 DTCFDLSNMNEVKVPTVVLHFR-GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGN 476

Query: 471 AQQQGTRVSFDLANSLVGFSSNKC 485
            QQQG RV +DLA+S VGF+   C
Sbjct: 477 IQQQGFRVVYDLASSRVGFAPGGC 484

[Close]
BLAST of CmaCh04G015250 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 4.4e-70
Identity = 144/355 (40.56%), Postives = 199/355 (56.06%), Query Frame = 0 

Query: 136 SPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADCYEQTDPIFEPTS 195
           S + +    G GEY   + IG P  P   ++DTGSD+ W QC PC  C+ Q+ PIF P  
Sbjct: 82  SGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQG 141

Query: 196 SASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETVTLGSTSLTNIAL 255
           S+SF++L C +Q C++L    C N  C Y   YGDGS T G   TET+T GS S+ NI  
Sbjct: 142 SSSFSTLPCSSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITF 201

Query: 256 GCGHNNEGLFIG-AAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSESTSTLDFNS---PI 315
           GCG NN+G   G  AGL+G+G G LS PSQL+ + FSYC+    S + S L   S    +
Sbjct: 202 GCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSV 261

Query: 316 PPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQM-SQDGNGGIIIDSGTAVT 375
              +    L ++  + TF+Y+ + G+SVG   LPI  ++F + S +G GGIIIDSGT +T
Sbjct: 262 TAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLT 321

Query: 376 RLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDL-SSKSRVEVPTVSFHFADGKELPL 435
                AY  +R  F+ + +        + FD C+   S  S +++PT   HF DG +L L
Sbjct: 322 YFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHF-DGGDLEL 381

Query: 436 PAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFSSNKC 485
           P++NY I   S G  C A   +   +SI GN QQQ   V +D  NS+V F+S +C
Sbjct: 382 PSENYFIS-PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]
BLAST of CmaCh04G015250 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 3.1e-68
Identity = 147/356 (41.29%), Postives = 198/356 (55.62%), Query Frame = 0 

Query: 135 ESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADCYEQTDPIFEPT 194
           E+P+ +    G GEY   V IG P S    ++DTGSD+ W QC PC  C+ Q  PIF P 
Sbjct: 86  ETPVYA----GDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQ 145

Query: 195 SSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETVTLGSTSLTNIA 254
            S+SF++L C++Q C+ L    C N  C Y   YGDGS T G   TET T  ++S+ NIA
Sbjct: 146 DSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETSSVPNIA 205

Query: 255 LGCGHNNEGLFIG-AAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSESTSTLDFNSP--- 314
            GCG +N+G   G  AGL+G+G G LS PSQL    FSYC+    S S STL   S    
Sbjct: 206 FGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASG 265

Query: 315 IPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGGIIIDSGTAVT 374
           +P  + +  L  +    T++Y+ + G++VGG+ L IP ++FQ+  DG GG+IIDSGT +T
Sbjct: 266 VPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLT 325

Query: 375 RLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDL-SSKSRVEVPTVSFHFADGKELPL 434
            L   AYN +  AF  + +        +   TC+   S  S V+VP +S  F DG  L L
Sbjct: 326 YLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQF-DGGVLNL 385

Query: 435 PAKNYLIPVDSEGTFCFAFAPTDST-LSILGNAQQQGTRVSFDLANSLVGFSSNKC 485
             +N LI   +EG  C A   +    +SI GN QQQ T+V +DL N  V F   +C
Sbjct: 386 GEQNILIS-PAEGVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]
BLAST of CmaCh04G015250 vs. ExPASy TrEMBL
Match: A0A6J1J6A9 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482126 PE=3 SV=1)

HSP 1 Score: 953.0 bits (2462), Expect = 4.9e-274
Identity = 484/484 (100.00%), Postives = 484/484 (100.00%), Query Frame = 0 

Query: 1   MATFLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDESTV 60
           MATFLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDESTV
Sbjct: 1   MATFLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDESTV 60

Query: 61  SDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEP 120
           SDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEP
Sbjct: 61  SDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEP 120

Query: 121 LPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPC 180
           LPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPC
Sbjct: 121 LPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPC 180

Query: 181 ADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT 240
           ADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT
Sbjct: 181 ADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT 240

Query: 241 ETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSE 300
           ETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSE
Sbjct: 241 ETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSE 300

Query: 301 STSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGG 360
           STSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGG
Sbjct: 301 STSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGG 360

Query: 361 IIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFH 420
           IIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFH
Sbjct: 361 IIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFH 420

Query: 421 FADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFS 480
           FADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFS
Sbjct: 421 FADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFS 480

Query: 481 SNKC 485
           SNKC
Sbjct: 481 SNKC 484

[Close]
BLAST of CmaCh04G015250 vs. ExPASy TrEMBL
Match: A0A6J1GZC0 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458894 PE=3 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 1.7e-266
Identity = 475/485 (97.94%), Postives = 476/485 (98.14%), Query Frame = 0 

Query: 1   MAT-FLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDEST 60
           MAT F LFLLPLLYASFS VLSR+LPHAPQTSVLDV ASIHTTRQVFAFQPQSPMRDEST
Sbjct: 1   MATAFFLFLLPLLYASFSGVLSRSLPHAPQTSVLDVDASIHTTRQVFAFQPQSPMRDEST 60

Query: 61  VSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLE 120
           VSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAI GITGADLE
Sbjct: 61  VSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLE 120

Query: 121 PLPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180
           PL  GG SQFG EDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP
Sbjct: 121 PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180

Query: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240
           CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV
Sbjct: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240

Query: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300
           TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS
Sbjct: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300

Query: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360
           ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG
Sbjct: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360

Query: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420
           GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF
Sbjct: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420

Query: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480
           HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF
Sbjct: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480

Query: 481 SSNKC 485
           SSNKC
Sbjct: 481 SSNKC 485

[Close]
BLAST of CmaCh04G015250 vs. ExPASy TrEMBL
Match: A0A1S3BFC3 (protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo OX=3656 GN=LOC103489261 PE=3 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.2e-240
Identity = 426/486 (87.65%), Postives = 452/486 (93.00%), Query Frame = 0 

Query: 1   MATFLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDESTV 60
           MAT  LFLL LL++S SA L RTLP  P+TSVLDVAASI  T+Q+FA +P+S   DE TV
Sbjct: 1   MATISLFLLSLLFSSLSAFLCRTLPPTPRTSVLDVAASIQRTQQIFAMEPKSSTPDEITV 60

Query: 61  SDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEP 120
           SDSSSL+L LNSR+S+MKTSH+DYKSLTLSRL RDSARV+SLTAR+DLAI GITG DLEP
Sbjct: 61  SDSSSLSLQLNSRISVMKTSHSDYKSLTLSRLKRDSARVKSLTARIDLAIRGITGTDLEP 120

Query: 121 LPT--GGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCA 180
           L    GG SQFG EDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCA
Sbjct: 121 LGNGDGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCA 180

Query: 181 PCADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDF 240
           PCA+CYEQTDPIFEPTSSASFTSLSC+T+QCKSLDVSECRNGTCLYEVSYGDGSYTVGDF
Sbjct: 181 PCAECYEQTDPIFEPTSSASFTSLSCETEQCKSLDVSECRNGTCLYEVSYGDGSYTVGDF 240

Query: 241 VTETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRD 300
           VTETVTLGSTSL NIA+GCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRD
Sbjct: 241 VTETVTLGSTSLRNIAIGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRD 300

Query: 301 SESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGN 360
           S+STSTLDFNSPI PDAVTAPLHRNPNLDTFFYLG+TGMSVGG +LPIPETSFQMS+DGN
Sbjct: 301 SDSTSTLDFNSPISPDAVTAPLHRNPNLDTFFYLGLTGMSVGGTVLPIPETSFQMSEDGN 360

Query: 361 GGIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVS 420
           GGII+DSGTAVTRLQTT YN+LRD+FVK THDLQSARGVALFDTCYDLSSKS VEVPTVS
Sbjct: 361 GGIIVDSGTAVTRLQTTVYNVLRDSFVKSTHDLQSARGVALFDTCYDLSSKSSVEVPTVS 420

Query: 421 FHFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVG 480
           FHFA+G ELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRV FDL+NSLVG
Sbjct: 421 FHFANGNELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLSNSLVG 480

Query: 481 FSSNKC 485
           FS NKC
Sbjct: 481 FSPNKC 486

[Close]
BLAST of CmaCh04G015250 vs. ExPASy TrEMBL
Match: A0A0A0KUG1 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G608450 PE=3 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 5.1e-239
Identity = 426/486 (87.65%), Postives = 449/486 (92.39%), Query Frame = 0 

Query: 1   MATFLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDESTV 60
           MAT  LFLL LL++S SA   RTL   P TSVLDVAASI  T+QVFA +P+S   DE+TV
Sbjct: 1   MATISLFLLSLLFSSLSAFHCRTLHPTPTTSVLDVAASIQRTQQVFAVEPKSSTPDETTV 60

Query: 61  SDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEP 120
           SD SSL+L LNSR+S+MK SH+DYKSLTLSRL RDSARVRSLTAR+DLAI GITG DLEP
Sbjct: 61  SDPSSLSLQLNSRISVMKASHSDYKSLTLSRLKRDSARVRSLTARIDLAIRGITGTDLEP 120

Query: 121 LPT--GGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCA 180
           L    GG SQFG EDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCA
Sbjct: 121 LGNGGGGGSQFGTEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCA 180

Query: 181 PCADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDF 240
           PCA+CYEQTDPIFEPTSSASFTSLSC+T+QCKSLDVSECRNGTCLYEVSYGDGSYTVGDF
Sbjct: 181 PCAECYEQTDPIFEPTSSASFTSLSCETEQCKSLDVSECRNGTCLYEVSYGDGSYTVGDF 240

Query: 241 VTETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRD 300
           VTETVTLGSTSL NIA+GCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRD
Sbjct: 241 VTETVTLGSTSLGNIAIGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRD 300

Query: 301 SESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGN 360
           S+STSTLDFNSPI PDAVTAPLHRNPNLDTFFYLG+TGMSVGG +LPIPETSFQMS+DGN
Sbjct: 301 SDSTSTLDFNSPITPDAVTAPLHRNPNLDTFFYLGLTGMSVGGAVLPIPETSFQMSEDGN 360

Query: 361 GGIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVS 420
           GGII+DSGTAVTRLQTT YN+LRDAFVK THDLQ+ARGVALFDTCYDLSSKSRVEVPTVS
Sbjct: 361 GGIIVDSGTAVTRLQTTVYNVLRDAFVKSTHDLQTARGVALFDTCYDLSSKSRVEVPTVS 420

Query: 421 FHFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVG 480
           FHFA+G ELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRV FDLANSLVG
Sbjct: 421 FHFANGNELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLANSLVG 480

Query: 481 FSSNKC 485
           FS NKC
Sbjct: 481 FSPNKC 486

[Close]
BLAST of CmaCh04G015250 vs. ExPASy TrEMBL
Match: A0A6J1G1W1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449928 PE=3 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 1.1e-233
Identity = 416/484 (85.95%), Postives = 441/484 (91.12%), Query Frame = 0 

Query: 1   MATFLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDESTV 60
           MA   LFLL  ++AS S  LSR+LPH  +TSVLDVAASI  T ++FAF+ +SP+ +E +V
Sbjct: 3   MAKIWLFLLIAVFASVSGGLSRSLPHRTKTSVLDVAASIQRTTEIFAFEVKSPVEEEKSV 62

Query: 61  SDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEP 120
            DSSSL L LNSR+SI+KTSH+DYKSLTLSRL RDSARVRSLTAR+DL I G TGADL  
Sbjct: 63  MDSSSLALQLNSRISIVKTSHSDYKSLTLSRLSRDSARVRSLTARIDLVIRGTTGADLRT 122

Query: 121 LPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPC 180
              GG S+F  EDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPC
Sbjct: 123 FGNGGGSRFRAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPC 182

Query: 181 ADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT 240
           ADCYEQTDPIFEPTSS SF SLSC+T+QCKSLDVSECRNGTCLYEVSYGDGS TVGDFVT
Sbjct: 183 ADCYEQTDPIFEPTSSTSFASLSCETEQCKSLDVSECRNGTCLYEVSYGDGSSTVGDFVT 242

Query: 241 ETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSE 300
           ETVTLGSTSL+NIALGCGHNNEGLF+GAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS+
Sbjct: 243 ETVTLGSTSLSNIALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSD 302

Query: 301 STSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGG 360
           S STLDFNSPIPPDAV APLHRNPNLDTFFYLG+TG+SVGGEILPIPETSFQMSQDGNGG
Sbjct: 303 SASTLDFNSPIPPDAVIAPLHRNPNLDTFFYLGVTGLSVGGEILPIPETSFQMSQDGNGG 362

Query: 361 IIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFH 420
           IIIDSGTAVTRLQTT YNLLRDAFVK T  LQSARGVALFDTCYDLSSKSRVEVPTVSFH
Sbjct: 363 IIIDSGTAVTRLQTTVYNLLRDAFVKSTPYLQSARGVALFDTCYDLSSKSRVEVPTVSFH 422

Query: 421 FADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFS 480
           FA+GKELPLPAKNYL+PVDSEGTFCFAFAPTDS LSILGNAQQQGTRVSFDL NSLVGFS
Sbjct: 423 FANGKELPLPAKNYLLPVDSEGTFCFAFAPTDSALSILGNAQQQGTRVSFDLTNSLVGFS 482

Query: 481 SNKC 485
            NKC
Sbjct: 483 PNKC 486

[Close]
BLAST of CmaCh04G015250 vs. NCBI nr
Match: XP_022983595.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima])

HSP 1 Score: 953.0 bits (2462), Expect = 1.0e-273
Identity = 484/484 (100.00%), Postives = 484/484 (100.00%), Query Frame = 0 

Query: 1   MATFLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDESTV 60
           MATFLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDESTV
Sbjct: 1   MATFLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDESTV 60

Query: 61  SDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEP 120
           SDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEP
Sbjct: 61  SDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEP 120

Query: 121 LPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPC 180
           LPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPC
Sbjct: 121 LPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPC 180

Query: 181 ADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT 240
           ADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT
Sbjct: 181 ADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT 240

Query: 241 ETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSE 300
           ETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSE
Sbjct: 241 ETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSE 300

Query: 301 STSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGG 360
           STSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGG
Sbjct: 301 STSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGG 360

Query: 361 IIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFH 420
           IIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFH
Sbjct: 361 IIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFH 420

Query: 421 FADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFS 480
           FADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFS
Sbjct: 421 FADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFS 480

Query: 481 SNKC 485
           SNKC
Sbjct: 481 SNKC 484

[Close]
BLAST of CmaCh04G015250 vs. NCBI nr
Match: XP_022957517.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata])

HSP 1 Score: 927.9 bits (2397), Expect = 3.5e-266
Identity = 475/485 (97.94%), Postives = 476/485 (98.14%), Query Frame = 0 

Query: 1   MAT-FLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDEST 60
           MAT F LFLLPLLYASFS VLSR+LPHAPQTSVLDV ASIHTTRQVFAFQPQSPMRDEST
Sbjct: 1   MATAFFLFLLPLLYASFSGVLSRSLPHAPQTSVLDVDASIHTTRQVFAFQPQSPMRDEST 60

Query: 61  VSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLE 120
           VSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAI GITGADLE
Sbjct: 61  VSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLE 120

Query: 121 PLPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180
           PL  GG SQFG EDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP
Sbjct: 121 PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180

Query: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240
           CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV
Sbjct: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240

Query: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300
           TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS
Sbjct: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300

Query: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360
           ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG
Sbjct: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360

Query: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420
           GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF
Sbjct: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420

Query: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480
           HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF
Sbjct: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480

Query: 481 SSNKC 485
           SSNKC
Sbjct: 481 SSNKC 485

[Close]
BLAST of CmaCh04G015250 vs. NCBI nr
Match: KAG6601306.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032091.1 Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 925.6 bits (2391), Expect = 1.7e-265
Identity = 474/485 (97.73%), Postives = 475/485 (97.94%), Query Frame = 0 

Query: 1   MAT-FLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDEST 60
           MAT F LFLLPLLYASFS VLSRTLPHAP+TSVLDV ASIHTTRQVFAFQPQSPMRDEST
Sbjct: 1   MATAFFLFLLPLLYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQPQSPMRDEST 60

Query: 61  VSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLE 120
           VSDSSSLTLLLNSRVSIMKTSH DYKSLTLSRLYRDSARVRSLTARMDLAI GITGADLE
Sbjct: 61  VSDSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLE 120

Query: 121 PLPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180
           PL  GG SQFG EDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP
Sbjct: 121 PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180

Query: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240
           CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV
Sbjct: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240

Query: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300
           TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS
Sbjct: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300

Query: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360
           ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG
Sbjct: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360

Query: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420
           GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF
Sbjct: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420

Query: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480
           HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF
Sbjct: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480

Query: 481 SSNKC 485
           SSNKC
Sbjct: 481 SSNKC 485

[Close]
BLAST of CmaCh04G015250 vs. NCBI nr
Match: XP_023544196.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 912.5 bits (2357), Expect = 1.5e-261
Identity = 470/485 (96.91%), Postives = 472/485 (97.32%), Query Frame = 0 

Query: 1   MAT-FLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDEST 60
           MAT F LFLLPLLYASFS VLSRTLPHA  TSVLDVAASIHTTRQVFAFQPQSPMRDE  
Sbjct: 1   MATAFFLFLLPLLYASFSGVLSRTLPHASGTSVLDVAASIHTTRQVFAFQPQSPMRDE-- 60

Query: 61  VSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLE 120
            SDSSSLTLLLNSRVSI+KTSHTDYKSLTLSRL+RDSARVRSLTARMDLAI GITGADLE
Sbjct: 61  -SDSSSLTLLLNSRVSIIKTSHTDYKSLTLSRLHRDSARVRSLTARMDLAIRGITGADLE 120

Query: 121 PLPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180
           PL  GG SQFG EDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP
Sbjct: 121 PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180

Query: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240
           CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV
Sbjct: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240

Query: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300
           TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS
Sbjct: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300

Query: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360
           ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG
Sbjct: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360

Query: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420
           GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF
Sbjct: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420

Query: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480
           HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF
Sbjct: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480

Query: 481 SSNKC 485
           SSNKC
Sbjct: 481 SSNKC 482

[Close]
BLAST of CmaCh04G015250 vs. NCBI nr
Match: XP_038891764.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida])

HSP 1 Score: 845.5 bits (2183), Expect = 2.3e-241
Identity = 429/484 (88.64%), Postives = 451/484 (93.18%), Query Frame = 0 

Query: 1   MATFLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDESTV 60
           MA   LFLLPLL++S S  L R+LP  P T+VLDVA SI  T+Q+FA  P+S M DE+TV
Sbjct: 23  MANISLFLLPLLFSSLSGFLCRSLPPTPTTAVLDVATSIQRTQQIFALDPESSMTDENTV 82

Query: 61  SDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEP 120
           SDSSSLTL L+SR+S+MKTSH+DYKSLTLSRL RDSARVRSLTARMDLAI GITGADLEP
Sbjct: 83  SDSSSLTLQLHSRISVMKTSHSDYKSLTLSRLKRDSARVRSLTARMDLAIRGITGADLEP 142

Query: 121 LPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPC 180
                  QFG EDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPC
Sbjct: 143 -------QFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPC 202

Query: 181 ADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT 240
           A+CYEQTDPIFEPTSSASFTSLSC+T+QCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT
Sbjct: 203 AECYEQTDPIFEPTSSASFTSLSCETEQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT 262

Query: 241 ETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSE 300
           ETVT GSTSL NIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS+
Sbjct: 263 ETVTFGSTSLRNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSD 322

Query: 301 STSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGG 360
           STSTLDFNSPIPPDAVTAPLHRNPNL+TFFYLG+TGMSVGG+ILPIPE+SFQMSQDGNGG
Sbjct: 323 STSTLDFNSPIPPDAVTAPLHRNPNLNTFFYLGVTGMSVGGDILPIPESSFQMSQDGNGG 382

Query: 361 IIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFH 420
           IIIDSGTAVTRLQTTAYN+LRDAFVK THDLQSARGVALFDTCYDLSSKSRVEVPTVSFH
Sbjct: 383 IIIDSGTAVTRLQTTAYNVLRDAFVKSTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFH 442

Query: 421 FADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFS 480
           FA+GKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRV FDLANSLVGFS
Sbjct: 443 FANGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLANSLVGFS 499

Query: 481 SNKC 485
            NKC
Sbjct: 503 PNKC 499

[Close]
BLAST of CmaCh04G015250 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 628.2 bits (1619), Expect = 5.3e-180
Identity = 316/483 (65.42%), Postives = 388/483 (80.33%), Query Frame = 0 

Query: 4   FLLFLLPLLYASFSAVLSRTLPH--APQTSVLDVAASIHTTRQVFAFQPQSPMRDESTVS 63
           +  F       S S+V SR LP      TS+L+VA SIH T+   +F+     ++E T S
Sbjct: 5   YSFFFFIFFLTSHSSVFSRILPETSTTTTSILNVADSIHRTKYTSSFRLN--QQEEQTHS 64

Query: 64  DSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEPL 123
            SSS +L L+SRVS+  T H+DYKSLTL+RL RD+ARV+SL  R+DLAI  I+ ADL+P+
Sbjct: 65  ASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKADLKPI 124

Query: 124 PTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCA 183
            T   ++   +D E+P++SG +QGSGEYF+RVGIG+P   VYMVLDTGSDV+W+QC PCA
Sbjct: 125 STMYTTE--EQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCA 184

Query: 184 DCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTE 243
           DCY QT+PIFEP+SS+S+  LSC T QC +L+VSECRN TCLYEVSYGDGSYTVGDF TE
Sbjct: 185 DCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGDFATE 244

Query: 244 TVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSES 303
           T+T+GST + N+A+GCGH+NEGLF+GAAGLLGLGGG L+ PSQLN +SFSYCLVDRDS+S
Sbjct: 245 TLTIGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDRDSDS 304

Query: 304 TSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGGI 363
            ST+DF + + PDAV APL RN  LDTF+YLG+TG+SVGGE+L IP++SF+M + G+GGI
Sbjct: 305 ASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESGSGGI 364

Query: 364 IIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFHF 423
           IIDSGTAVTRLQT  YN LRD+FVK T DL+ A GVA+FDTCY+LS+K+ VEVPTV+FHF
Sbjct: 365 IIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVPTVAFHF 424

Query: 424 ADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFSS 483
             GK L LPAKNY+IPVDS GTFC AFAPT S+L+I+GN QQQGTRV+FDLANSL+GFSS
Sbjct: 425 PGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSLIGFSS 483

Query: 484 NKC 485
           NKC
Sbjct: 485 NKC 483

[Close]
BLAST of CmaCh04G015250 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 533.5 bits (1373), Expect = 1.8e-151
Identity = 266/485 (54.85%), Postives = 355/485 (73.20%), Query Frame = 0 

Query: 12  LYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQP---------QSPMRDESTVSD 71
           L+ + +   SR+L   P+T+VLDV +S+  T+ + +  P            + D    + 
Sbjct: 17  LFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPESLSDPVFFNS 76

Query: 72  SSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEPLP 131
           SS L+L L+SR + + + H DYKSLTLSRL RDS+RV  + A++  A+ G+  +DL+P+ 
Sbjct: 77  SSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDLKPV- 136

Query: 132 TGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCAD 191
               +++  ED  +P+VSGASQGSGEYFSR+G+G P   +Y+VLDTGSDV+W+QC PCAD
Sbjct: 137 YNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCAD 196

Query: 192 CYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTET 251
           CY+Q+DP+F PTSS+++ SL+C   QC  L+ S CR+  CLY+VSYGDGS+TVG+  T+T
Sbjct: 197 CYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDT 256

Query: 252 VTLG-STSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSES 311
           VT G S  + N+ALGCGH+NEGLF GAAGLLGLGGG LS  +Q+ A+SFSYCLVDRDS  
Sbjct: 257 VTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGK 316

Query: 312 TSTLDFNS-PIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGG 371
           +S+LDFNS  +     TAPL RN  +DTF+Y+G++G SVGGE + +P+  F +   G+GG
Sbjct: 317 SSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGG 376

Query: 372 IIIDSGTAVTRLQTTAYNLLRDAFVKKTHDL-QSARGVALFDTCYDLSSKSRVEVPTVSF 431
           +I+D GTAVTRLQT AYN LRDAF+K T +L + +  ++LFDTCYD SS S V+VPTV+F
Sbjct: 377 VILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAF 436

Query: 432 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 485
           HF  GK L LPAKNYLIPVD  GTFCFAFAPT S+LSI+GN QQQGTR+++DL+ +++G 
Sbjct: 437 HFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGL 496

[Close]
BLAST of CmaCh04G015250 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 403.7 bits (1036), Expect = 2.1e-112
Identity = 219/485 (45.15%), Postives = 299/485 (61.65%), Query Frame = 0 

Query: 4   FLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDESTVSDS 63
           F  FL   L+ S S+ +S      P   ++DV     T          +   DES    S
Sbjct: 6   FFFFLHLHLHLSSSSSIS-----FPDFQIIDVLQPPLTVTATLPDFNNTHFSDES----S 65

Query: 64  SSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEPLPT 123
           S  TL L  R      ++ ++     +R+ RD+ RV ++  R+   +  I  +D      
Sbjct: 66  SKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKV--IPSSD------ 125

Query: 124 GGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADC 183
              S++ + DF S IVSG  QGSGEYF R+G+G PP   YMV+D+GSD+ WVQC PC  C
Sbjct: 126 ---SRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLC 185

Query: 184 YEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETV 243
           Y+Q+DP+F+P  S S+T +SC +  C  ++ S C +G C YEV YGDGSYT G    ET+
Sbjct: 186 YKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETL 245

Query: 244 TLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLN---ASSFSYCLVDRDSE 303
           T   T + N+A+GCGH N G+FIGAAGLLG+GGGS+SF  QL+     +F YCLV R ++
Sbjct: 246 TFAKTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTD 305

Query: 304 STSTLDF-NSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 363
           ST +L F    +P  A   PL RNP   +F+Y+G+ G+ VGG  +P+P+  F +++ G+G
Sbjct: 306 STGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDG 365

Query: 364 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 423
           G+++D+GTAVTRL T AY   RD F  +T +L  A GV++FDTCYDLS    V VPTVSF
Sbjct: 366 GVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSF 425

Query: 424 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 483
           +F +G  L LPA+N+L+PVD  GT+CFAFA + + LSI+GN QQ+G +VSFD AN  VGF
Sbjct: 426 YFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGF 470

Query: 484 SSNKC 485
             N C
Sbjct: 486 GPNVC 470

[Close]
BLAST of CmaCh04G015250 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 403.3 bits (1035), Expect = 2.8e-112
Identity = 231/470 (49.15%), Postives = 297/470 (63.19%), Query Frame = 0 

Query: 29  QTSVLDVAASIHTTRQVFAFQPQSPMRDESTVSDSSSLTLLLNSRVSIMKTSHTDYKSLT 88
           QT V++   S  T     ++     + DES    ++SL++ L+   ++   S      L 
Sbjct: 28  QTLVVNTLPSSAT----LSWPESESLTDESLSESTTSLSVHLSHVDALSSFSDASPADLF 87

Query: 89  LSRLYRDSARVRSLTARMDLAIPGITGADLEPLPTGGASQFGMEDFESPIVSGASQGSGE 148
             RL RDS RV+S+T+ +     G       P   GG        F   ++SG SQGSGE
Sbjct: 88  NLRLQRDSLRVKSITS-LAAVSTGRNATKRTPRTAGG--------FSGAVISGLSQGSGE 147

Query: 149 YFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADCYEQTDPIFEPTSSASFTSLSCQTQQ 208
           YF R+G+G P + VYMVLDTGSDV W+QC+PC  CY QTD IF+P  S +F ++ C ++ 
Sbjct: 148 YFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIFDPKKSKTFATVPCGSRL 207

Query: 209 CKSL-DVSEC---RNGTCLYEVSYGDGSYTVGDFVTETVTLGSTSLTNIALGCGHNNEGL 268
           C+ L D SEC   R+ TCLY+VSYGDGS+T GDF TET+T     + ++ LGCGH+NEGL
Sbjct: 208 CRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTFHGARVDHVPLGCGHDNEGL 267

Query: 269 FIGAAGLLGLGGGSLSFPSQLN---ASSFSYCLVDRDSEST-----STLDF-NSPIPPDA 328
           F+GAAGLLGLG G LSFPSQ        FSYCLVDR S  +     ST+ F N+ +P  +
Sbjct: 268 FVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTS 327

Query: 329 VTAPLHRNPNLDTFFYLGMTGMSVGGEILP-IPETSFQMSQDGNGGIIIDSGTAVTRLQT 388
           V  PL  NP LDTF+YL + G+SVGG  +P + E+ F++   GNGG+IIDSGT+VTRL  
Sbjct: 328 VFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQ 387

Query: 389 TAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFHFADGKELPLPAKNY 448
            AY  LRDAF      L+ A   +LFDTC+DLS  + V+VPTV FHF  G E+ LPA NY
Sbjct: 388 PAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTVVFHFGGG-EVSLPASNY 447

Query: 449 LIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFSSNKC 485
           LIPV++EG FCFAFA T  +LSI+GN QQQG RV++DL  S VGF S  C
Sbjct: 448 LIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

[Close]
BLAST of CmaCh04G015250 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 398.7 bits (1023), Expect = 6.8e-111
Identity = 227/444 (51.13%), Postives = 288/444 (64.86%), Query Frame = 0 

Query: 51  QSPMRDESTVSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAI 110
           +S     S    SSS+TL L+   ++      D   L  SRL RDS RV+S+ A +   I
Sbjct: 57  ESEFESGSDSESSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSI-ATLAAQI 116

Query: 111 PG--ITGADLEPLPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDT 170
           PG  +T A   P P G         F S +VSG SQGSGEYF+R+G+G P   VYMVLDT
Sbjct: 117 PGRNVTHA---PRPGG---------FSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDT 176

Query: 171 GSDVSWVQCAPCADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSEC--RNGTCLYEV 230
           GSD+ W+QCAPC  CY Q+DPIF+P  S ++ ++ C +  C+ LD + C  R  TCLY+V
Sbjct: 177 GSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQV 236

Query: 231 SYGDGSYTVGDFVTETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLN 290
           SYGDGS+TVGDF TET+T     +  +ALGCGH+NEGLF+GAAGLLGLG G LSFP Q  
Sbjct: 237 SYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTG 296

Query: 291 ---ASSFSYCLVDRDSES--TSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGG 350
                 FSYCLVDR + S  +S +  N+ +   A   PL  NP LDTF+Y+G+ G+SVGG
Sbjct: 297 HRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGG 356

Query: 351 EILP-IPETSFQMSQDGNGGIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALF 410
             +P +  + F++ Q GNGG+IIDSGT+VTRL   AY  +RDAF      L+ A   +LF
Sbjct: 357 TRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLF 416

Query: 411 DTCYDLSSKSRVEVPTVSFHFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGN 470
           DTC+DLS+ + V+VPTV  HF  G ++ LPA NYLIPVD+ G FCFAFA T   LSI+GN
Sbjct: 417 DTCFDLSNMNEVKVPTVVLHFR-GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGN 476

Query: 471 AQQQGTRVSFDLANSLVGFSSNKC 485
            QQQG RV +DLA+S VGF+   C
Sbjct: 477 IQQQGFRVVYDLASSRVGFAPGGC 484

The following BLAST results are available for this feature:
BLAST of CmaCh04G015250 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LS402.5e-15054.85Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LHE33.0e-11145.15Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LNJ39.6e-11051.13Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q766C34.4e-7040.56Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C23.1e-6841.29Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
BLAST of CmaCh04G015250 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1J6A94.9e-274100.00protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GZC01.7e-26697.94protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A1S3BFC31.2e-24087.65protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo OX=3656 GN=LOC10348926... [more]
A0A0A0KUG15.1e-23987.65Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G60845... [more]
A0A6J1G1W11.1e-23385.95protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=... [more]
BLAST of CmaCh04G015250 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022983595.11.0e-273100.00protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima][more]
XP_022957517.13.5e-26697.94protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata][more]
KAG6601306.11.7e-26597.73Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp... [more]
XP_023544196.11.5e-26196.91protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo][more]
XP_038891764.12.3e-24188.64protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida][more]
BLAST of CmaCh04G015250 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G25510.15.3e-18065.42Eukaryotic aspartyl protease family protein [more]
AT3G18490.11.8e-15154.85Eukaryotic aspartyl protease family protein [more]
AT3G20015.12.1e-11245.15Eukaryotic aspartyl protease family protein [more]
AT3G61820.12.8e-11249.15Eukaryotic aspartyl protease family protein [more]
AT1G01300.16.8e-11151.13Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 330..480
e-value: 5.5E-36
score: 123.8
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 149..306
e-value: 7.7E-49
score: 166.3
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 126..307
e-value: 6.9E-51
score: 175.0
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 311..484
e-value: 4.8E-51
score: 175.1
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 143..484
NoneNo IPR availablePANTHERPTHR13683:SF775EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 10..484
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 10..484
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 164..175
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 149..480
score: 44.833229

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G015250.1CmaCh04G015250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity