CmaCh04G000370 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G000370
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionXyloglucan endotransglucosylase/hydrolase
LocationCma_Chr04: 207985 .. 210428 (-)
RNA-Seq ExpressionCmaCh04G000370
SyntenyCmaCh04G000370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCATCGGTGCTTGCCCTTCCCTTCCACTTACATTACACGCCAACAAAGCGCCTCTTCTGCTCTTCTGCGACCTTTTTCTACCCATTTTCTTCCCTCTTTTCCCACCCCTCTTTTTCTCTTCTTCTTTTCTCCCCATGGCTCTCTCTTCCTTCTCCTTCCTCTGTTTTCCCCTTTCCTTCATTCTTCTCCTCCTTCCCTCTTCTTCTTCCCCTCCAAATCTTCCAATCCTCTTCTTCGACGAAGCCTACACCCACTTGTTCGGCGATGACAATCTCCTTGTTCTCAAGGATGGAAAATCGGTGCATCTGCATCTAGATGAACGAACAGGTTTTCTCTTTCTCTCTTTCTTACTTCCCTTTCATTTTCTCTCTTTGTTGCTGGTTTTTTAATGTGGGTTTTTTTTTTTTTTTTTCTTTCAGGCGCGGGATTTGTGTCCCAGGACCTTTATCTTCATGGGTTCTTCAGTGCTTCCATTAAATTGCCTGCGGATTACTCTGCTGGTGTTGTGGTTGCTTTTTATGTGAGTATGGAAATTGGAATTCATTGGGTTTTTTTTTCTGGAAATGGAAATGGGTTGAAGTGTACTCTGTTTAAGTTCTTAATAATCTTTAACTGTTTCAATCCATGAGTTTCTCATGATTTGACAATATGGGTGTTTCTCTTCATTTGATTTTGTTCTGTATTCTTTCACTCTGTTTTCCGTGTGTATTAGTGGGGCTGTTAATGTGTTAGGGTTGTGATAAAGAACTTACTAAACACAACTTACTTCAGGGGTATTAATATGGAAGATCTTTTAAAACTTAACCTTTAGCAGTTTTTTATTTTTTATTTTCCCTTTAATTATTATTATTATTATAAGAAATATCGCATTTTAATACTTGTTTCTACCCTCTGAAAATGGTTTTAAGTTCTCATCCAACAAACACTTTCAGAGTTTTTAAATGTGATTAAATACATTGCTTTTAAGGGGTTACCTAACAAACACTTCAGTTCTTAGACACAAGCATAGGGCACCTAACAAACACTTTTCGTGTTCAAAGTGTTTTTGGAGAGAATTATAGCAAATCCCAAACATACTCTTAGTTGTTCAGGTTAAAATTTTAACTTCCATTGCAGTGAATTGGACGTTGGACTCAACCTGTAAGTTGAGTTGAATTTGTTCAGTTGTTGGGGTAATTTATTCAGAATTTTGTTAAAATGTAGCGGAAATGTTAGTAACTGGGGTTAGTAACTCAATAGAGTAACTGTGGGATGACAGATGTCGAATGGCGATATGTTTGAGAACAACCACGATGAGATCGATTTCGAGTTCTTAGGGAATATCAGAGGAAAAGAATGGAGGGTTCAGACTAACTTTTACGGCAATGGAAGCACCAACGTTGGCAGAGAAGAAAGATATGGTCTTTGGTTTGATCCAGCTGAAGATTTTCATCAGTACAGCATTCTTTGGACTGATTCTGTCATTATGTAAGCTATTTCATATGTTTTTTTTTTCTTCTCAGAGAGTTTGGAATCTCCCATGTTTTGTTTTGTTCTTTTAATGATGTTTTTCCAGTGCCATTAGCCATTTGGAAATTAGATTCAGAAAGAAAATGATTTTTTGCTCCCTCCTCCCCCTATGGTTTGTTCGGTGCCGGCTGTTTTTATTGATGTTCTAAAACTTGTTCTTTCTTTTGGTTCCTAAACTTTCATATGTTTTGTTACAATGGTGTCTAAGCTTTCAGATGTTCTGAATGTTCATTCAACTAGTTTGTAAAAAGAATGAATCATTGATATACTGCTGAATGCTTCCACTGTTTTGTCATATGAAGATTTTATGTGGATGAAGTTCCTATAAGAGAAGTTAAAAGAACAGCTTCAATGGGCGGTGAGTTTCCATCCAAGCCAATGACTTTGTATGCAACAATTTGGGATGGTTCAGGCTGGGCCACCAATGGAGGCAAATACAAGGTAAACTACAAATATGCACCCTATGTCGCCCAGTTCTCCAAATTTGTATTGCATGGTTGCACAGTCGATCCGATTGATCTGCACTTCTCCAAGTGCGACGACCTTACACAGAACTCCGAAGCCACCATCCCCAATGACATCACAACTGCTAAAACAAGAAAAATGGAGAGTTTCAGGAGGAAGCACATGACGTACTCATATTGCTATGATCGTCTTCGATACAAGATCCCTCCAGCAGAATGTGTCATTGTACCTCAAGAAGCCGAGCGACTTCGAAAGTTTGACCCTGTAACATTTGGAAAAGGGCGGCGGCACCACCACCGTGGGAAACACCATCACTCTAGCCAGGCTGAGTTNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCAATAATTTCCGGCTGTTTGATTTAGATGGGTCTGTTCTTTTTTGTGTATAAAAATATAGAGACAGTTGGAGGAGTATAG

mRNA sequence

TCCATCGGTGCTTGCCCTTCCCTTCCACTTACATTACACGCCAACAAAGCGCCTCTTCTGCTCTTCTGCGACCTTTTTCTACCCATTTTCTTCCCTCTTTTCCCACCCCTCTTTTTCTCTTCTTCTTTTCTCCCCATGGCTCTCTCTTCCTTCTCCTTCCTCTGTTTTCCCCTTTCCTTCATTCTTCTCCTCCTTCCCTCTTCTTCTTCCCCTCCAAATCTTCCAATCCTCTTCTTCGACGAAGCCTACACCCACTTGTTCGGCGATGACAATCTCCTTGTTCTCAAGGATGGAAAATCGGTGCATCTGCATCTAGATGAACGAACAGGCGCGGGATTTGTGTCCCAGGACCTTTATCTTCATGGGTTCTTCAGTGCTTCCATTAAATTGCCTGCGGATTACTCTGCTGGTGTTGTGGTTGCTTTTTATATGTCGAATGGCGATATGTTTGAGAACAACCACGATGAGATCGATTTCGAGTTCTTAGGGAATATCAGAGGAAAAGAATGGAGGGTTCAGACTAACTTTTACGGCAATGGAAGCACCAACGTTGGCAGAGAAGAAAGATATGGTCTTTGGTTTGATCCAGCTGAAGATTTTCATCAGTACAGCATTCTTTGGACTGATTCTGTCATTATATTTTATGTGGATGAAGTTCCTATAAGAGAAGTTAAAAGAACAGCTTCAATGGGCGGTGAGTTTCCATCCAAGCCAATGACTTTGTATGCAACAATTTGGGATGGTTCAGGCTGGGCCACCAATGGAGGCAAATACAAGGTAAACTACAAATATGCACCCTATGTCGCCCAGTTCTCCAAATTTGTATTGCATGGTTGCACAGTCGATCCGATTGATCTGCACTTCTCCAAGTGCGACGACCTTACACAGAACTCCGAAGCCACCATCCCCAATGACATCACAACTGCTAAAACAAGAAAAATGGAGAGTTTCAGGAGGAAGCACATGACGTACTCATATTGCTATGATCGTCTTCGATACAAGATCCCTCCAGCAGAATGTGTCATTGTACCTCAAGAAGCCGAGCGACTTCGAAAGTTTGACCCTGTAACATTTGGAAAAGGGCGGCGGCACCACCACCAGACAGTTGGAGGAGTATAG

Coding sequence (CDS)

ATGGCTCTCTCTTCCTTCTCCTTCCTCTGTTTTCCCCTTTCCTTCATTCTTCTCCTCCTTCCCTCTTCTTCTTCCCCTCCAAATCTTCCAATCCTCTTCTTCGACGAAGCCTACACCCACTTGTTCGGCGATGACAATCTCCTTGTTCTCAAGGATGGAAAATCGGTGCATCTGCATCTAGATGAACGAACAGGCGCGGGATTTGTGTCCCAGGACCTTTATCTTCATGGGTTCTTCAGTGCTTCCATTAAATTGCCTGCGGATTACTCTGCTGGTGTTGTGGTTGCTTTTTATATGTCGAATGGCGATATGTTTGAGAACAACCACGATGAGATCGATTTCGAGTTCTTAGGGAATATCAGAGGAAAAGAATGGAGGGTTCAGACTAACTTTTACGGCAATGGAAGCACCAACGTTGGCAGAGAAGAAAGATATGGTCTTTGGTTTGATCCAGCTGAAGATTTTCATCAGTACAGCATTCTTTGGACTGATTCTGTCATTATATTTTATGTGGATGAAGTTCCTATAAGAGAAGTTAAAAGAACAGCTTCAATGGGCGGTGAGTTTCCATCCAAGCCAATGACTTTGTATGCAACAATTTGGGATGGTTCAGGCTGGGCCACCAATGGAGGCAAATACAAGGTAAACTACAAATATGCACCCTATGTCGCCCAGTTCTCCAAATTTGTATTGCATGGTTGCACAGTCGATCCGATTGATCTGCACTTCTCCAAGTGCGACGACCTTACACAGAACTCCGAAGCCACCATCCCCAATGACATCACAACTGCTAAAACAAGAAAAATGGAGAGTTTCAGGAGGAAGCACATGACGTACTCATATTGCTATGATCGTCTTCGATACAAGATCCCTCCAGCAGAATGTGTCATTGTACCTCAAGAAGCCGAGCGACTTCGAAAGTTTGACCCTGTAACATTTGGAAAAGGGCGGCGGCACCACCACCAGACAGTTGGAGGAGTATAG

Protein sequence

MALSSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHLDERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNIRGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVKRTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVDPIDLHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQEAERLRKFDPVTFGKGRRHHHQTVGGV
Homology
BLAST of CmaCh04G000370 vs. ExPASy Swiss-Prot
Match: Q38909 (Probable xyloglucan endotransglucosylase/hydrolase protein 28 OS=Arabidopsis thaliana OX=3702 GN=XTH28 PE=2 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 1.8e-123
Identity = 214/315 (67.94%), Postives = 255/315 (80.95%), Query Frame = 0

Query: 8   FLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHLDERTGAG 67
           FL F   F  L+  S  +   LP++ FDE YT LFGD NL+V +DGKSV L LDERTG+G
Sbjct: 7   FLVFMSLFTSLV--SGFALQKLPLIQFDEGYTQLFGDQNLIVHRDGKSVRLTLDERTGSG 66

Query: 68  FVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNIRGKEWRV 127
           FVS D+YLHGFFS+SIKLPADYSAGVV+AFY+SNGD++E NHDEIDFEFLGNIRG+EWR+
Sbjct: 67  FVSNDIYLHGFFSSSIKLPADYSAGVVIAFYLSNGDLYEKNHDEIDFEFLGNIRGREWRI 126

Query: 128 QTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVKRTASMGG 187
           QTN YGNGST++GREERY LWFDP EDFHQYSILW+ S IIFYVD VPIREVKRTASMGG
Sbjct: 127 QTNIYGNGSTHLGREERYNLWFDPTEDFHQYSILWSLSHIIFYVDNVPIREVKRTASMGG 186

Query: 188 EFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVDPIDLHFSKCD 247
           +FP+KPM+LY+TIWDGS WAT+GGKY VNYKYAPYV+QF+  +LHGC VDP +   S  D
Sbjct: 187 DFPAKPMSLYSTIWDGSKWATDGGKYGVNYKYAPYVSQFTDLILHGCAVDPTEKFPSCKD 246

Query: 248 DLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQEAERLRK 307
           +  QN    + ++IT ++  KME FR+KHMTYSYCYD +RYK+  +ECV+ P EA+RLR 
Sbjct: 247 EAVQN--LRLASEITESQRNKMEIFRQKHMTYSYCYDHMRYKVVLSECVVNPAEAKRLRV 306

Query: 308 FDPVTFGKGRRHHHQ 323
           +DPVTFG G  H H+
Sbjct: 307 YDPVTFG-GIPHGHR 316

BLAST of CmaCh04G000370 vs. ExPASy Swiss-Prot
Match: Q8LDS2 (Probable xyloglucan endotransglucosylase/hydrolase protein 27 OS=Arabidopsis thaliana OX=3702 GN=XTH27 PE=2 SV=2)

HSP 1 Score: 439.1 bits (1128), Expect = 4.3e-122
Identity = 212/301 (70.43%), Postives = 240/301 (79.73%), Query Frame = 0

Query: 20  LPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHLDERTGAGFVSQDLYLHGFF 79
           L S  +  NLPI  F+E+YT LFGD NL V +DGKSV L LDERTG+GFVS D YLHGFF
Sbjct: 17  LVSGFALQNLPITSFEESYTQLFGDKNLFVHQDGKSVRLTLDERTGSGFVSNDYYLHGFF 76

Query: 80  SASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNIRGKEWRVQTNFYGNGSTNV 139
           SASIKLP+DY+AGVVVAFYMSNGDM+E NHDEIDFEFLGNIR KEWRVQTN YGNGST+ 
Sbjct: 77  SASIKLPSDYTAGVVVAFYMSNGDMYEKNHDEIDFEFLGNIREKEWRVQTNIYGNGSTHS 136

Query: 140 GREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVKRTASMGGEFPSKPMTLYAT 199
           GREERY LWFDP EDFHQYSILW+DS IIF+VD VPIREVKRTA MGG FPSKPM+LY T
Sbjct: 137 GREERYNLWFDPTEDFHQYSILWSDSHIIFFVDNVPIREVKRTAEMGGHFPSKPMSLYTT 196

Query: 200 IWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVDPIDLHFSKCDDLTQNSEATIPN 259
           IWDGS WATNGGKY VNYKYAPY+A+FS  VLHGC VDPI+  F +CD+     +     
Sbjct: 197 IWDGSKWATNGGKYGVNYKYAPYIARFSDLVLHGCPVDPIE-QFPRCDE-GAAEDMRAAQ 256

Query: 260 DITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQEAERLRKFDPVTFGKGRRH 319
           +IT ++  KM+ FRR+ MTYSYCYDR RY +  +ECV+ P EA+RLR +DPV FG   R 
Sbjct: 257 EITPSQRSKMDVFRRRLMTYSYCYDRARYNVALSECVVNPAEAQRLRVYDPVRFGGIPRR 315

Query: 320 H 321
           H
Sbjct: 317 H 315

BLAST of CmaCh04G000370 vs. ExPASy Swiss-Prot
Match: Q38908 (Probable xyloglucan endotransglucosylase/hydrolase protein 30 OS=Arabidopsis thaliana OX=3702 GN=XTH30 PE=2 SV=2)

HSP 1 Score: 355.9 bits (912), Expect = 4.8e-97
Identity = 179/317 (56.47%), Postives = 225/317 (70.98%), Query Frame = 0

Query: 1   MALSSFSFLCFPLSFILLL---LPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVH 60
           M+ SS++ +     FIL+L   L SSS+  NL  L F+E+ + LFGD NL+   D  SV 
Sbjct: 1   MSKSSYNHI-----FILILCLCLRSSSAFTNLNTLSFEESLSPLFGDANLVRSPDDLSVR 60

Query: 61  LHLDERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFL 120
           L LD  TG+GF+S ++Y HGF+S+ IKLPADY+AGVVVAFY SNGD+FE  HDE+D EFL
Sbjct: 61  LLLDRYTGSGFISSNMYQHGFYSSMIKLPADYTAGVVVAFYTSNGDVFEKTHDELDIEFL 120

Query: 121 GNIRGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIR 180
           GNI+GK WR QTN YGNGST+ GREERY LWFDP+++FH+YSILWT   IIF+VD+VPIR
Sbjct: 121 GNIKGKPWRFQTNLYGNGSTHRGREERYRLWFDPSKEFHRYSILWTPHKIIFWVDDVPIR 180

Query: 181 EVKRTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVD 240
           EV R  +MG ++P+KPM LYATIWD S WAT+GGKYK NYK+AP+VA+F  F L GC+VD
Sbjct: 181 EVIRNDAMGADYPAKPMALYATIWDASDWATSGGKYKANYKFAPFVAEFKSFSLDGCSVD 240

Query: 241 PIDLHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVI 300
           PI      C D     E+   + I + +   M  FR++ M YSYCYD LRY  P  ECVI
Sbjct: 241 PIQEVPMDCSDSVDFLESQDYSSINSHQRAAMRRFRQRFMYYSYCYDTLRYPEPLPECVI 300

Query: 301 VPQEAERLRKFDPVTFG 315
           VP E +R ++   + FG
Sbjct: 301 VPAEKDRFKETGRLKFG 312

BLAST of CmaCh04G000370 vs. ExPASy Swiss-Prot
Match: Q8L7H3 (Probable xyloglucan endotransglucosylase/hydrolase protein 29 OS=Arabidopsis thaliana OX=3702 GN=XTH29 PE=2 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 4.8e-89
Identity = 156/306 (50.98%), Postives = 208/306 (67.97%), Query Frame = 0

Query: 28  NLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHLDERTGAGFVSQDLYLHGFFSASIKLPA 87
           N+  +FFDE  +HLFG+ NL+   D +SV L LD+ TG+GF+S  +Y HGFFS+ IKLP 
Sbjct: 34  NINPIFFDEGLSHLFGEGNLIRSPDDRSVRLLLDKYTGSGFISSSMYQHGFFSSLIKLPG 93

Query: 88  DYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNIRGKEWRVQTNFYGNGSTNVGREERYGL 147
            Y+AG+VVAFY SNGD+F  +HDE+D EFLGN+ GK WR QTN YGNGSTN GREERY L
Sbjct: 94  AYTAGIVVAFYTSNGDVFVKDHDELDIEFLGNLEGKPWRFQTNMYGNGSTNRGREERYRL 153

Query: 148 WFDPAEDFHQYSILWTDSVIIFYVDEVPIREVKRTASMGGEFPSKPMTLYATIWDGSGWA 207
           WFDP+++FH+YSILWT + IIF+VD+VPIRE+ R   M G++P KPM+LYATIWD S WA
Sbjct: 154 WFDPSKEFHRYSILWTPTKIIFWVDDVPIREILRKEEMNGDYPQKPMSLYATIWDASSWA 213

Query: 208 TNGGKYKVNYKYAPYVAQFSKFVLHGCTVD---PIDLHFSKCDDLTQ-----NSEATIPN 267
           T+GGK+ V+Y ++P+V++F    L GC V    P + + +   +        + +  + N
Sbjct: 214 TSGGKFGVDYTFSPFVSEFKDIALDGCNVSDSFPGENNNNNIGNYNNINCSVSDQFLMSN 273

Query: 268 DITTAKTRK---MESFRRKHMTYSYCYDRLRYKIPPAECVIVPQEAERLRKFDPVTFGKG 323
           D +T   ++   M  FR ++M YSYCYD +RY +PP ECVIV  E  R R    + FG  
Sbjct: 274 DYSTISPKQATAMRRFRERYMYYSYCYDTIRYSVPPPECVIVTAEKNRFRDTGRLKFGGS 333

BLAST of CmaCh04G000370 vs. ExPASy Swiss-Prot
Match: Q8LC45 (Probable xyloglucan endotransglucosylase/hydrolase protein 33 OS=Arabidopsis thaliana OX=3702 GN=XTH33 PE=1 SV=2)

HSP 1 Score: 245.7 bits (626), Expect = 7.0e-64
Identity = 120/272 (44.12%), Postives = 164/272 (60.29%), Query Frame = 0

Query: 35  DEAYTHLFGDDNLLVLKDGKSVHLHLDERTGAGFVSQDLYLHGFFSASIKLPADYSAGVV 94
           +  ++  FG  N+ V  +G    L LD+ +GAG VS++ Y +GFFSA +KLPA +++GVV
Sbjct: 44  ENGFSRRFGAHNIQV--NGSLAKLTLDKSSGAGLVSKNKYHYGFFSARLKLPAGFASGVV 103

Query: 95  VAFYMSNGDMFENNHDEIDFEFLGNIRGKEWRVQTNFYGNGSTNVGREERYGLWFDPAED 154
           VAFY+SN + +  +HDEID E LG  R  +W +QTN Y NGST  GREE++  WFDP + 
Sbjct: 104 VAFYLSNAETYPKSHDEIDIELLGRSRRDDWTIQTNVYANGSTRTGREEKFYFWFDPTQA 163

Query: 155 FHQYSILWTDSVIIFYVDEVPIREVKRTASMGGEFPSKPMTLYATIWDGSGWATNGGKYK 214
           FH Y+++W     +F VD +P+R+     +    +PSKPM+LY T+WDGS WAT GGKY 
Sbjct: 164 FHDYTLIWNSHHTVFLVDNIPVRQFPNRGAFTSAYPSKPMSLYVTVWDGSEWATKGGKYP 223

Query: 215 VNYKYAPYVAQFSKFVLHGCTVD-----------PIDLHFSKCDDLTQNSEATIPNDITT 274
           VNYKYAP+V   +   L GC+V+                 S  D +     AT+  +   
Sbjct: 224 VNYKYAPFVVSVADVELSGCSVNNGSSTGSGPCTKSGGSISSLDPVDGQDFATLSKNQIN 283

Query: 275 AKTRKMESFRRKHMTYSYCYDRLRYKIPPAEC 296
           A    M+  RRK M YSYC D+ RYK+ PAEC
Sbjct: 284 A----MDWARRKLMFYSYCSDKPRYKVMPAEC 309

BLAST of CmaCh04G000370 vs. ExPASy TrEMBL
Match: A0A6J1JKQ1 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111485295 PE=3 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 5.6e-189
Identity = 321/322 (99.69%), Postives = 322/322 (100.00%), Query Frame = 0

Query: 1   MALSSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL 60
           MALSSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL
Sbjct: 1   MALSSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL 60

Query: 61  DERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI 120
           DERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI
Sbjct: 61  DERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI 120

Query: 121 RGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK 180
           RGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK
Sbjct: 121 RGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK 180

Query: 181 RTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVDPID 240
           RTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVDPID
Sbjct: 181 RTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVDPID 240

Query: 241 LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ 300
           LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ
Sbjct: 241 LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ 300

Query: 301 EAERLRKFDPVTFGKGRRHHHQ 323
           EAERLRKFDPVTFGKGRRHHH+
Sbjct: 301 EAERLRKFDPVTFGKGRRHHHR 322

BLAST of CmaCh04G000370 vs. ExPASy TrEMBL
Match: A0A6J1FUV1 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111447484 PE=3 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 3.7e-185
Identity = 314/320 (98.12%), Postives = 317/320 (99.06%), Query Frame = 0

Query: 1   MALSSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL 60
           MA SSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL
Sbjct: 1   MAFSSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL 60

Query: 61  DERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI 120
           D+RTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI
Sbjct: 61  DQRTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI 120

Query: 121 RGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK 180
           RGKEWRVQTN YGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK
Sbjct: 121 RGKEWRVQTNIYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK 180

Query: 181 RTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVDPID 240
           RTASMGG+FPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPY+AQFSKFVLHGCTVDPID
Sbjct: 181 RTASMGGKFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYIAQFSKFVLHGCTVDPID 240

Query: 241 LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ 300
           LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ
Sbjct: 241 LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ 300

Query: 301 EAERLRKFDPVTFGKGRRHH 321
           EAERLRKFDPVTFGKGR HH
Sbjct: 301 EAERLRKFDPVTFGKGRPHH 320

BLAST of CmaCh04G000370 vs. ExPASy TrEMBL
Match: A0A1S3C021 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=LOC103495069 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.3e-160
Identity = 279/322 (86.65%), Postives = 294/322 (91.30%), Query Frame = 0

Query: 1   MALSSFSFLCFPL--SFILLLLP-SSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVH 60
           M  SSF+FLCF L  SF+LLLLP SSSSP NLP+L F EAY HLFGD NLL+L  GKSVH
Sbjct: 1   MGFSSFTFLCFSLLPSFLLLLLPFSSSSPSNLPVLSFHEAYNHLFGDANLLLLNHGKSVH 60

Query: 61  LHLDERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFL 120
           L+LDERTGAGF+SQDLYLHGFFSASIKLPADY+AGVVVAFYMSNGDMFENNHDEIDFEFL
Sbjct: 61  LYLDERTGAGFLSQDLYLHGFFSASIKLPADYTAGVVVAFYMSNGDMFENNHDEIDFEFL 120

Query: 121 GNIRGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIR 180
           GNIRGKEWRVQTN YGNGSTNVGREERYGLWFDPAEDFHQYSILWTDS IIFYVDEVPIR
Sbjct: 121 GNIRGKEWRVQTNIYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSRIIFYVDEVPIR 180

Query: 181 EVKRTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVD 240
           EVKRTASMGGEFPSKPMTLYATIWDGS WATNGGKYKVNYKY+PY+A+FS+FVLHGCTVD
Sbjct: 181 EVKRTASMGGEFPSKPMTLYATIWDGSSWATNGGKYKVNYKYSPYIAEFSEFVLHGCTVD 240

Query: 241 PIDLHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVI 300
           PI+  F KCD   Q SEA IPN+ITTAK  KMESFRRKHMTYSYCYD LRYKIPPAECV+
Sbjct: 241 PIEQVFRKCDLTPQISEAAIPNNITTAKRTKMESFRRKHMTYSYCYDHLRYKIPPAECVL 300

Query: 301 VPQEAERLRKFDPVTFGKGRRH 320
            PQEA+RLRKFDPVTFGKGRRH
Sbjct: 301 DPQEAKRLRKFDPVTFGKGRRH 322

BLAST of CmaCh04G000370 vs. ExPASy TrEMBL
Match: A0A0A0KMP2 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G057140 PE=3 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.5e-157
Identity = 277/323 (85.76%), Postives = 292/323 (90.40%), Query Frame = 0

Query: 1   MALSSFSFLCFPLSFILLLLPSSS---SPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVH 60
           M  SSF+FL F     LLLLP SS   SP NLPIL F EAY HLFGD NLL+L  GKSVH
Sbjct: 1   MGFSSFTFLSF-----LLLLPFSSPSPSPSNLPILSFHEAYNHLFGDANLLLLNHGKSVH 60

Query: 61  LHLDERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFL 120
           LHLDERTGAGF+SQDLYLHGFFSASIKLPADY+AGVVVAFYMSNGDMFENNHDEIDFEFL
Sbjct: 61  LHLDERTGAGFLSQDLYLHGFFSASIKLPADYTAGVVVAFYMSNGDMFENNHDEIDFEFL 120

Query: 121 GNIRGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIR 180
           GNIRGKEWRVQTN YGNGSTNVGREERYGLWFDPAEDFHQYSILWTDS IIFYVDEVPIR
Sbjct: 121 GNIRGKEWRVQTNIYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSRIIFYVDEVPIR 180

Query: 181 EVKRTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVD 240
           EVKRTASMGGEFPSKPMTLYATIWDGS WATNGGKYKVNYKYAPY+A+FS+FVLHGCTVD
Sbjct: 181 EVKRTASMGGEFPSKPMTLYATIWDGSSWATNGGKYKVNYKYAPYIAEFSEFVLHGCTVD 240

Query: 241 PIDLHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVI 300
           PI+  F KC DLTQ SEA+IPN+ITTAK  KMESFRRKHMTYSYCYD LRYKIPPAECV+
Sbjct: 241 PIEQVFPKC-DLTQISEASIPNNITTAKRTKMESFRRKHMTYSYCYDHLRYKIPPAECVL 300

Query: 301 VPQEAERLRKFDPVTFGKGRRHH 321
            PQEA+RL++FDPVTFG+GRRHH
Sbjct: 301 DPQEAKRLQRFDPVTFGRGRRHH 317

BLAST of CmaCh04G000370 vs. ExPASy TrEMBL
Match: N0DX43 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth31 PE=3 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.3e-156
Identity = 276/323 (85.45%), Postives = 291/323 (90.09%), Query Frame = 0

Query: 1   MALSSFSFLCFPLSFILLLLPSSS---SPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVH 60
           M  SSF+FL F     LLLLP SS   SP NLPIL F EAY HLFGD NLL+L  GKSVH
Sbjct: 1   MGFSSFTFLSF-----LLLLPFSSPSPSPSNLPILSFHEAYNHLFGDANLLLLNHGKSVH 60

Query: 61  LHLDERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFL 120
           LHLDERTGAGF+SQDLYLHGFFSASIKLPADY+AGVVVAFYMSNGDMFENNHDEIDFEFL
Sbjct: 61  LHLDERTGAGFLSQDLYLHGFFSASIKLPADYTAGVVVAFYMSNGDMFENNHDEIDFEFL 120

Query: 121 GNIRGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIR 180
           GNIRGKEWRVQTN YGNGSTNVGREERYGLWFDPAED HQYSILWTDS IIFYVDEVPIR
Sbjct: 121 GNIRGKEWRVQTNIYGNGSTNVGREERYGLWFDPAEDLHQYSILWTDSRIIFYVDEVPIR 180

Query: 181 EVKRTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVD 240
           EVKRTASMGGEFPSKPMTLYATIWDGS WATNGGKYKVNYKYAPY+A+FS+FVLHGCTVD
Sbjct: 181 EVKRTASMGGEFPSKPMTLYATIWDGSSWATNGGKYKVNYKYAPYIAEFSEFVLHGCTVD 240

Query: 241 PIDLHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVI 300
           PI+  F KC DLTQ SEA+IPN+ITTAK  KMESFRRKHMTYSYCYD LRYKIPPAECV+
Sbjct: 241 PIEQVFPKC-DLTQISEASIPNNITTAKRTKMESFRRKHMTYSYCYDHLRYKIPPAECVL 300

Query: 301 VPQEAERLRKFDPVTFGKGRRHH 321
            PQEA+RL++FDPVTFG+GRRHH
Sbjct: 301 DPQEAKRLQRFDPVTFGRGRRHH 317

BLAST of CmaCh04G000370 vs. NCBI nr
Match: XP_022987893.1 (probable xyloglucan endotransglucosylase/hydrolase protein 28 [Cucurbita maxima])

HSP 1 Score: 669.8 bits (1727), Expect = 1.2e-188
Identity = 321/322 (99.69%), Postives = 322/322 (100.00%), Query Frame = 0

Query: 1   MALSSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL 60
           MALSSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL
Sbjct: 1   MALSSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL 60

Query: 61  DERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI 120
           DERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI
Sbjct: 61  DERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI 120

Query: 121 RGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK 180
           RGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK
Sbjct: 121 RGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK 180

Query: 181 RTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVDPID 240
           RTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVDPID
Sbjct: 181 RTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVDPID 240

Query: 241 LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ 300
           LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ
Sbjct: 241 LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ 300

Query: 301 EAERLRKFDPVTFGKGRRHHHQ 323
           EAERLRKFDPVTFGKGRRHHH+
Sbjct: 301 EAERLRKFDPVTFGKGRRHHHR 322

BLAST of CmaCh04G000370 vs. NCBI nr
Match: XP_023513342.1 (probable xyloglucan endotransglucosylase/hydrolase protein 28 [Cucurbita pepo subsp. pepo] >XP_023513354.1 probable xyloglucan endotransglucosylase/hydrolase protein 28 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 658.7 bits (1698), Expect = 2.7e-185
Identity = 315/320 (98.44%), Postives = 317/320 (99.06%), Query Frame = 0

Query: 1   MALSSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL 60
           MA SSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGD NLLVLKDGKSVHLHL
Sbjct: 1   MAFSSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDHNLLVLKDGKSVHLHL 60

Query: 61  DERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI 120
           DERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI
Sbjct: 61  DERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI 120

Query: 121 RGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK 180
           RGKEWRVQTN YGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK
Sbjct: 121 RGKEWRVQTNIYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK 180

Query: 181 RTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVDPID 240
           RTASMGG+FPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPY+AQFSKFVLHGCTVDPID
Sbjct: 181 RTASMGGDFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYIAQFSKFVLHGCTVDPID 240

Query: 241 LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ 300
           LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ
Sbjct: 241 LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ 300

Query: 301 EAERLRKFDPVTFGKGRRHH 321
           EAERLRKFDPVTFGKGRRHH
Sbjct: 301 EAERLRKFDPVTFGKGRRHH 320

BLAST of CmaCh04G000370 vs. NCBI nr
Match: KAG6599882.1 (putative xyloglucan endotransglucosylase/hydrolase protein 28, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 658.3 bits (1697), Expect = 3.5e-185
Identity = 315/320 (98.44%), Postives = 316/320 (98.75%), Query Frame = 0

Query: 1   MALSSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL 60
           MA SS SFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL
Sbjct: 1   MAFSSLSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL 60

Query: 61  DERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI 120
           DERTGAGFVS DLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI
Sbjct: 61  DERTGAGFVSHDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI 120

Query: 121 RGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK 180
           RGKEWRVQTN YGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK
Sbjct: 121 RGKEWRVQTNIYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK 180

Query: 181 RTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVDPID 240
           RTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPY+AQFSKFVLHGCTVDPID
Sbjct: 181 RTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYIAQFSKFVLHGCTVDPID 240

Query: 241 LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ 300
           LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ
Sbjct: 241 LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ 300

Query: 301 EAERLRKFDPVTFGKGRRHH 321
           EAERLRKFDPVTFGKGRRHH
Sbjct: 301 EAERLRKFDPVTFGKGRRHH 320

BLAST of CmaCh04G000370 vs. NCBI nr
Match: XP_022942448.1 (probable xyloglucan endotransglucosylase/hydrolase protein 28 [Cucurbita moschata])

HSP 1 Score: 657.1 bits (1694), Expect = 7.7e-185
Identity = 314/320 (98.12%), Postives = 317/320 (99.06%), Query Frame = 0

Query: 1   MALSSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL 60
           MA SSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL
Sbjct: 1   MAFSSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL 60

Query: 61  DERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI 120
           D+RTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI
Sbjct: 61  DQRTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI 120

Query: 121 RGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK 180
           RGKEWRVQTN YGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK
Sbjct: 121 RGKEWRVQTNIYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK 180

Query: 181 RTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVDPID 240
           RTASMGG+FPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPY+AQFSKFVLHGCTVDPID
Sbjct: 181 RTASMGGKFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYIAQFSKFVLHGCTVDPID 240

Query: 241 LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ 300
           LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ
Sbjct: 241 LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ 300

Query: 301 EAERLRKFDPVTFGKGRRHH 321
           EAERLRKFDPVTFGKGR HH
Sbjct: 301 EAERLRKFDPVTFGKGRPHH 320

BLAST of CmaCh04G000370 vs. NCBI nr
Match: KAG7030567.1 (putative xyloglucan endotransglucosylase/hydrolase protein 28 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 655.2 bits (1689), Expect = 2.9e-184
Identity = 314/320 (98.12%), Postives = 315/320 (98.44%), Query Frame = 0

Query: 1   MALSSFSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL 60
           MA SS SFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL
Sbjct: 1   MAFSSLSFLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHL 60

Query: 61  DERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI 120
           DERTGAGFVS DLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI
Sbjct: 61  DERTGAGFVSHDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNI 120

Query: 121 RGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK 180
           RGKEWRVQTN YGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK
Sbjct: 121 RGKEWRVQTNIYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVK 180

Query: 181 RTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVDPID 240
           RTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPY+AQFSKFVLHGCTVDPID
Sbjct: 181 RTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYIAQFSKFVLHGCTVDPID 240

Query: 241 LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQ 300
           LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIV Q
Sbjct: 241 LHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVSQ 300

Query: 301 EAERLRKFDPVTFGKGRRHH 321
           EAERLRKFDPVTFGKGRRHH
Sbjct: 301 EAERLRKFDPVTFGKGRRHH 320

BLAST of CmaCh04G000370 vs. TAIR 10
Match: AT1G14720.1 (xyloglucan endotransglucosylase/hydrolase 28 )

HSP 1 Score: 443.7 bits (1140), Expect = 1.3e-124
Identity = 214/315 (67.94%), Postives = 255/315 (80.95%), Query Frame = 0

Query: 8   FLCFPLSFILLLLPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHLDERTGAG 67
           FL F   F  L+  S  +   LP++ FDE YT LFGD NL+V +DGKSV L LDERTG+G
Sbjct: 7   FLVFMSLFTSLV--SGFALQKLPLIQFDEGYTQLFGDQNLIVHRDGKSVRLTLDERTGSG 66

Query: 68  FVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNIRGKEWRV 127
           FVS D+YLHGFFS+SIKLPADYSAGVV+AFY+SNGD++E NHDEIDFEFLGNIRG+EWR+
Sbjct: 67  FVSNDIYLHGFFSSSIKLPADYSAGVVIAFYLSNGDLYEKNHDEIDFEFLGNIRGREWRI 126

Query: 128 QTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVKRTASMGG 187
           QTN YGNGST++GREERY LWFDP EDFHQYSILW+ S IIFYVD VPIREVKRTASMGG
Sbjct: 127 QTNIYGNGSTHLGREERYNLWFDPTEDFHQYSILWSLSHIIFYVDNVPIREVKRTASMGG 186

Query: 188 EFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVDPIDLHFSKCD 247
           +FP+KPM+LY+TIWDGS WAT+GGKY VNYKYAPYV+QF+  +LHGC VDP +   S  D
Sbjct: 187 DFPAKPMSLYSTIWDGSKWATDGGKYGVNYKYAPYVSQFTDLILHGCAVDPTEKFPSCKD 246

Query: 248 DLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQEAERLRK 307
           +  QN    + ++IT ++  KME FR+KHMTYSYCYD +RYK+  +ECV+ P EA+RLR 
Sbjct: 247 EAVQN--LRLASEITESQRNKMEIFRQKHMTYSYCYDHMRYKVVLSECVVNPAEAKRLRV 306

Query: 308 FDPVTFGKGRRHHHQ 323
           +DPVTFG G  H H+
Sbjct: 307 YDPVTFG-GIPHGHR 316

BLAST of CmaCh04G000370 vs. TAIR 10
Match: AT2G01850.1 (endoxyloglucan transferase A3 )

HSP 1 Score: 439.1 bits (1128), Expect = 3.1e-123
Identity = 212/301 (70.43%), Postives = 240/301 (79.73%), Query Frame = 0

Query: 20  LPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHLDERTGAGFVSQDLYLHGFF 79
           L S  +  NLPI  F+E+YT LFGD NL V +DGKSV L LDERTG+GFVS D YLHGFF
Sbjct: 17  LVSGFALQNLPITSFEESYTQLFGDKNLFVHQDGKSVRLTLDERTGSGFVSNDYYLHGFF 76

Query: 80  SASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNIRGKEWRVQTNFYGNGSTNV 139
           SASIKLP+DY+AGVVVAFYMSNGDM+E NHDEIDFEFLGNIR KEWRVQTN YGNGST+ 
Sbjct: 77  SASIKLPSDYTAGVVVAFYMSNGDMYEKNHDEIDFEFLGNIREKEWRVQTNIYGNGSTHS 136

Query: 140 GREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIREVKRTASMGGEFPSKPMTLYAT 199
           GREERY LWFDP EDFHQYSILW+DS IIF+VD VPIREVKRTA MGG FPSKPM+LY T
Sbjct: 137 GREERYNLWFDPTEDFHQYSILWSDSHIIFFVDNVPIREVKRTAEMGGHFPSKPMSLYTT 196

Query: 200 IWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVDPIDLHFSKCDDLTQNSEATIPN 259
           IWDGS WATNGGKY VNYKYAPY+A+FS  VLHGC VDPI+  F +CD+     +     
Sbjct: 197 IWDGSKWATNGGKYGVNYKYAPYIARFSDLVLHGCPVDPIE-QFPRCDE-GAAEDMRAAQ 256

Query: 260 DITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVIVPQEAERLRKFDPVTFGKGRRH 319
           +IT ++  KM+ FRR+ MTYSYCYDR RY +  +ECV+ P EA+RLR +DPV FG   R 
Sbjct: 257 EITPSQRSKMDVFRRRLMTYSYCYDRARYNVALSECVVNPAEAQRLRVYDPVRFGGIPRR 315

Query: 320 H 321
           H
Sbjct: 317 H 315

BLAST of CmaCh04G000370 vs. TAIR 10
Match: AT1G32170.1 (xyloglucan endotransglucosylase/hydrolase 30 )

HSP 1 Score: 355.9 bits (912), Expect = 3.4e-98
Identity = 179/317 (56.47%), Postives = 225/317 (70.98%), Query Frame = 0

Query: 1   MALSSFSFLCFPLSFILLL---LPSSSSPPNLPILFFDEAYTHLFGDDNLLVLKDGKSVH 60
           M+ SS++ +     FIL+L   L SSS+  NL  L F+E+ + LFGD NL+   D  SV 
Sbjct: 1   MSKSSYNHI-----FILILCLCLRSSSAFTNLNTLSFEESLSPLFGDANLVRSPDDLSVR 60

Query: 61  LHLDERTGAGFVSQDLYLHGFFSASIKLPADYSAGVVVAFYMSNGDMFENNHDEIDFEFL 120
           L LD  TG+GF+S ++Y HGF+S+ IKLPADY+AGVVVAFY SNGD+FE  HDE+D EFL
Sbjct: 61  LLLDRYTGSGFISSNMYQHGFYSSMIKLPADYTAGVVVAFYTSNGDVFEKTHDELDIEFL 120

Query: 121 GNIRGKEWRVQTNFYGNGSTNVGREERYGLWFDPAEDFHQYSILWTDSVIIFYVDEVPIR 180
           GNI+GK WR QTN YGNGST+ GREERY LWFDP+++FH+YSILWT   IIF+VD+VPIR
Sbjct: 121 GNIKGKPWRFQTNLYGNGSTHRGREERYRLWFDPSKEFHRYSILWTPHKIIFWVDDVPIR 180

Query: 181 EVKRTASMGGEFPSKPMTLYATIWDGSGWATNGGKYKVNYKYAPYVAQFSKFVLHGCTVD 240
           EV R  +MG ++P+KPM LYATIWD S WAT+GGKYK NYK+AP+VA+F  F L GC+VD
Sbjct: 181 EVIRNDAMGADYPAKPMALYATIWDASDWATSGGKYKANYKFAPFVAEFKSFSLDGCSVD 240

Query: 241 PIDLHFSKCDDLTQNSEATIPNDITTAKTRKMESFRRKHMTYSYCYDRLRYKIPPAECVI 300
           PI      C D     E+   + I + +   M  FR++ M YSYCYD LRY  P  ECVI
Sbjct: 241 PIQEVPMDCSDSVDFLESQDYSSINSHQRAAMRRFRQRFMYYSYCYDTLRYPEPLPECVI 300

Query: 301 VPQEAERLRKFDPVTFG 315
           VP E +R ++   + FG
Sbjct: 301 VPAEKDRFKETGRLKFG 312

BLAST of CmaCh04G000370 vs. TAIR 10
Match: AT4G18990.1 (xyloglucan endotransglucosylase/hydrolase 29 )

HSP 1 Score: 329.3 bits (843), Expect = 3.4e-90
Identity = 156/306 (50.98%), Postives = 208/306 (67.97%), Query Frame = 0

Query: 28  NLPILFFDEAYTHLFGDDNLLVLKDGKSVHLHLDERTGAGFVSQDLYLHGFFSASIKLPA 87
           N+  +FFDE  +HLFG+ NL+   D +SV L LD+ TG+GF+S  +Y HGFFS+ IKLP 
Sbjct: 34  NINPIFFDEGLSHLFGEGNLIRSPDDRSVRLLLDKYTGSGFISSSMYQHGFFSSLIKLPG 93

Query: 88  DYSAGVVVAFYMSNGDMFENNHDEIDFEFLGNIRGKEWRVQTNFYGNGSTNVGREERYGL 147
            Y+AG+VVAFY SNGD+F  +HDE+D EFLGN+ GK WR QTN YGNGSTN GREERY L
Sbjct: 94  AYTAGIVVAFYTSNGDVFVKDHDELDIEFLGNLEGKPWRFQTNMYGNGSTNRGREERYRL 153

Query: 148 WFDPAEDFHQYSILWTDSVIIFYVDEVPIREVKRTASMGGEFPSKPMTLYATIWDGSGWA 207
           WFDP+++FH+YSILWT + IIF+VD+VPIRE+ R   M G++P KPM+LYATIWD S WA
Sbjct: 154 WFDPSKEFHRYSILWTPTKIIFWVDDVPIREILRKEEMNGDYPQKPMSLYATIWDASSWA 213

Query: 208 TNGGKYKVNYKYAPYVAQFSKFVLHGCTVD---PIDLHFSKCDDLTQ-----NSEATIPN 267
           T+GGK+ V+Y ++P+V++F    L GC V    P + + +   +        + +  + N
Sbjct: 214 TSGGKFGVDYTFSPFVSEFKDIALDGCNVSDSFPGENNNNNIGNYNNINCSVSDQFLMSN 273

Query: 268 DITTAKTRK---MESFRRKHMTYSYCYDRLRYKIPPAECVIVPQEAERLRKFDPVTFGKG 323
           D +T   ++   M  FR ++M YSYCYD +RY +PP ECVIV  E  R R    + FG  
Sbjct: 274 DYSTISPKQATAMRRFRERYMYYSYCYDTIRYSVPPPECVIVTAEKNRFRDTGRLKFGGS 333

BLAST of CmaCh04G000370 vs. TAIR 10
Match: AT1G10550.1 (xyloglucan:xyloglucosyl transferase 33 )

HSP 1 Score: 245.7 bits (626), Expect = 5.0e-65
Identity = 120/272 (44.12%), Postives = 164/272 (60.29%), Query Frame = 0

Query: 35  DEAYTHLFGDDNLLVLKDGKSVHLHLDERTGAGFVSQDLYLHGFFSASIKLPADYSAGVV 94
           +  ++  FG  N+ V  +G    L LD+ +GAG VS++ Y +GFFSA +KLPA +++GVV
Sbjct: 44  ENGFSRRFGAHNIQV--NGSLAKLTLDKSSGAGLVSKNKYHYGFFSARLKLPAGFASGVV 103

Query: 95  VAFYMSNGDMFENNHDEIDFEFLGNIRGKEWRVQTNFYGNGSTNVGREERYGLWFDPAED 154
           VAFY+SN + +  +HDEID E LG  R  +W +QTN Y NGST  GREE++  WFDP + 
Sbjct: 104 VAFYLSNAETYPKSHDEIDIELLGRSRRDDWTIQTNVYANGSTRTGREEKFYFWFDPTQA 163

Query: 155 FHQYSILWTDSVIIFYVDEVPIREVKRTASMGGEFPSKPMTLYATIWDGSGWATNGGKYK 214
           FH Y+++W     +F VD +P+R+     +    +PSKPM+LY T+WDGS WAT GGKY 
Sbjct: 164 FHDYTLIWNSHHTVFLVDNIPVRQFPNRGAFTSAYPSKPMSLYVTVWDGSEWATKGGKYP 223

Query: 215 VNYKYAPYVAQFSKFVLHGCTVD-----------PIDLHFSKCDDLTQNSEATIPNDITT 274
           VNYKYAP+V   +   L GC+V+                 S  D +     AT+  +   
Sbjct: 224 VNYKYAPFVVSVADVELSGCSVNNGSSTGSGPCTKSGGSISSLDPVDGQDFATLSKNQIN 283

Query: 275 AKTRKMESFRRKHMTYSYCYDRLRYKIPPAEC 296
           A    M+  RRK M YSYC D+ RYK+ PAEC
Sbjct: 284 A----MDWARRKLMFYSYCSDKPRYKVMPAEC 309

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q389091.8e-12367.94Probable xyloglucan endotransglucosylase/hydrolase protein 28 OS=Arabidopsis tha... [more]
Q8LDS24.3e-12270.43Probable xyloglucan endotransglucosylase/hydrolase protein 27 OS=Arabidopsis tha... [more]
Q389084.8e-9756.47Probable xyloglucan endotransglucosylase/hydrolase protein 30 OS=Arabidopsis tha... [more]
Q8L7H34.8e-8950.98Probable xyloglucan endotransglucosylase/hydrolase protein 29 OS=Arabidopsis tha... [more]
Q8LC457.0e-6444.12Probable xyloglucan endotransglucosylase/hydrolase protein 33 OS=Arabidopsis tha... [more]
Match NameE-valueIdentityDescription
A0A6J1JKQ15.6e-18999.69Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1FUV13.7e-18598.13Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A1S3C0219.3e-16086.65Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=LOC10349506... [more]
A0A0A0KMP22.5e-15785.76Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G05... [more]
N0DX431.3e-15685.45Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth31 PE... [more]
Match NameE-valueIdentityDescription
XP_022987893.11.2e-18899.69probable xyloglucan endotransglucosylase/hydrolase protein 28 [Cucurbita maxima][more]
XP_023513342.12.7e-18598.44probable xyloglucan endotransglucosylase/hydrolase protein 28 [Cucurbita pepo su... [more]
KAG6599882.13.5e-18598.44putative xyloglucan endotransglucosylase/hydrolase protein 28, partial [Cucurbit... [more]
XP_022942448.17.7e-18598.13probable xyloglucan endotransglucosylase/hydrolase protein 28 [Cucurbita moschat... [more]
KAG7030567.12.9e-18498.13putative xyloglucan endotransglucosylase/hydrolase protein 28 [Cucurbita argyros... [more]
Match NameE-valueIdentityDescription
AT1G14720.11.3e-12467.94xyloglucan endotransglucosylase/hydrolase 28 [more]
AT2G01850.13.1e-12370.43endoxyloglucan transferase A3 [more]
AT1G32170.13.4e-9856.47xyloglucan endotransglucosylase/hydrolase 30 [more]
AT4G18990.13.4e-9050.98xyloglucan endotransglucosylase/hydrolase 29 [more]
AT1G10550.15.0e-6544.12xyloglucan:xyloglucosyl transferase 33 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010713Xyloglucan endo-transglycosylase, C-terminalPFAMPF06955XET_Ccoord: 259..295
e-value: 1.7E-12
score: 47.0
IPR016455Xyloglucan endotransglucosylase/hydrolasePIRSFPIRSF005604EndGlu_transfcoord: 2..300
e-value: 3.9E-113
score: 375.4
IPR016455Xyloglucan endotransglucosylase/hydrolaseCDDcd02176GH16_XETcoord: 34..295
e-value: 7.26274E-137
score: 386.94
IPR000757Glycoside hydrolase family 16PFAMPF00722Glyco_hydro_16coord: 37..218
e-value: 2.2E-53
score: 180.4
IPR000757Glycoside hydrolase family 16PROSITEPS51762GH16_2coord: 21..226
score: 28.479883
NoneNo IPR availableGENE3D2.60.120.200coord: 26..295
e-value: 4.5E-90
score: 303.6
NoneNo IPR availablePANTHERPTHR31062:SF235XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 27-RELATEDcoord: 15..313
IPR044791Beta-glucanase/XTHPANTHERPTHR31062XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATEDcoord: 15..313
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 33..297

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G000370.1CmaCh04G000370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042546 cell wall biogenesis
biological_process GO:0071555 cell wall organization
biological_process GO:0010411 xyloglucan metabolic process
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006073 cellular glucan metabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0005618 cell wall
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity