CmaCh03G001390 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh03G001390 vs. ExPASy Swiss-Prot
Match: Q5YLB4 (DNA gyrase subunit B, chloroplastic/mitochondrial OS=Nicotiana benthamiana OX=4100 GN=GYRB PE=2 SV=1)

HSP 1 Score: 1154.4 bits (2985), Expect = 0.0e+00
Identity = 592/731 (80.98%), Postives = 644/731 (88.10%), Query Frame = 0

Query: 9   LLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPPFSFLEFSGTRFRARKIPTSLRL 68
           LLK  P  H     +S  L  S  H  +L+ S+      S +       + R++  ++ L
Sbjct: 3   LLKPFPLQHSLRCMASRFLLHSHYHTHTLSFSSISLSRPSIVLNPRVINKLRRLSDAI-L 62

Query: 69  AQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYIGSTGPRGL 128
            +N +VS  AFMSSS  T+    +  SK YGSEQIQVLEGLDPVRKRPGMYIGSTGPRGL
Sbjct: 63  IRN-MVSLRAFMSSSTTTEAFQENTKSKGYGSEQIQVLEGLDPVRKRPGMYIGSTGPRGL 122

Query: 129 HHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKKSSLETVLT 188
           HHLVYEILDNAVDEAQAGFA+KIDVVLHADNSVSI DNGRGIPT+LHPVTKKSSLETVLT
Sbjct: 123 HHLVYEILDNAVDEAQAGFATKIDVVLHADNSVSIADNGRGIPTELHPVTKKSSLETVLT 182

Query: 189 VLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYSRGKPATTL 248
           VLHAGGKFGGSSSGY+VSGGLHGVGLSVVNALS++LEV IWRDGKE+ QKYSRGKP TTL
Sbjct: 183 VLHAGGKFGGSSSGYNVSGGLHGVGLSVVNALSQALEVTIWRDGKEYQQKYSRGKPITTL 242

Query: 249 TCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITLTKEDD 308
            CH    E++D QGT IRFWPDKEVFTT +QFDYNTI+GRIRELAFLNP+LTI L KED 
Sbjct: 243 ICHDLPVEMRDRQGTAIRFWPDKEVFTTEMQFDYNTIAGRIRELAFLNPELTIALKKEDI 302

Query: 309 DVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWCADAYSDTI 368
           D EK Q NEY YAGGLVEYV WLN+DKKPLHDV GFRKE DG+ ID+ALQWC+DAYSDT+
Sbjct: 303 DPEKIQCNEYFYAGGLVEYVKWLNADKKPLHDVLGFRKEADGITIDMALQWCSDAYSDTM 362

Query: 369 LGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGLTCVVSVR 428
           LGYANSIRTIDGGTHIDG+KA+LTR LNNLGKKSK IK+KDISLSGEHVREGLTCV+SV+
Sbjct: 363 LGYANSIRTIDGGTHIDGVKAALTRILNNLGKKSKTIKEKDISLSGEHVREGLTCVISVK 422

Query: 429 VQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNALKAALAAK 488
           V NPEFEGQTKTRLGNPEVRKVV+QS+QEYLTEYLELHPDVLD ILSKSLNALKAALAAK
Sbjct: 423 VPNPEFEGQTKTRLGNPEVRKVVDQSVQEYLTEYLELHPDVLDSILSKSLNALKAALAAK 482

Query: 489 RARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDRRFQAILPL 548
           RARELVRQKSVL+SSSLPGKLADCS+TNPE++EIFIVEGDSAGGSAKQGRDRRFQAILPL
Sbjct: 483 RARELVRQKSVLKSSSLPGKLADCSATNPEEAEIFIVEGDSAGGSAKQGRDRRFQAILPL 542

Query: 549 RGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTDADVDGAHI 608
           RGKILNIERKDEAAMYKNEEIQNLIL LGLGVKGEDFKKEALRYHKIIILTDADVDGAHI
Sbjct: 543 RGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFKKEALRYHKIIILTDADVDGAHI 602

Query: 609 RTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRSFSSNASYN 668
           RTLLLTFFFRYQ+ALF+EGCIY+GVPPLYKVERGK+ YYCYDD ELK++QRSF SNASYN
Sbjct: 603 RTLLLTFFFRYQRALFEEGCIYVGVPPLYKVERGKQVYYCYDDAELKKVQRSFPSNASYN 662

Query: 669 IQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDFRKELIQKS 728
           IQRFKGLGEMMP QLWETTMNPE R+LKQL VEDAAEANVVFSSLMG+RVD RK+LIQ S
Sbjct: 663 IQRFKGLGEMMPAQLWETTMNPETRLLKQLVVEDAAEANVVFSSLMGSRVDIRKQLIQNS 722

Query: 729 ANMINLGHLDI 740
           A+M+NL  LDI
Sbjct: 723 ASMMNLEQLDI 731

BLAST of CmaCh03G001390 vs. ExPASy Swiss-Prot
Match: Q94BZ7 (DNA gyrase subunit B, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GYRBM PE=2 SV=1)

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 565/738 (76.56%), Postives = 630/738 (85.37%), Query Frame = 0

Query: 9   LLKTPPYL---HLRLMASSASLFPSS----IHLRSLTSSASRAPPFSFLEFSGTRFRARK 68
           LL+   YL   +LRLM S   LF SS    +H  S T S   +PPFS        FR + 
Sbjct: 3   LLQRASYLRLYYLRLMGSRPRLFSSSLSPALHRHSSTLS---SPPFS---SPSPSFRLKF 62

Query: 69  IPTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYIG 128
             TS+ L+Q  ++   A  S   +T+ S  + +SK Y SEQIQVLEGLDPVRKRPGMYIG
Sbjct: 63  QLTSV-LSQR-LIQRNAISSRFLSTEASQETTTSKGYSSEQIQVLEGLDPVRKRPGMYIG 122

Query: 129 STGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKKS 188
           STG RGLHHLVYEILDNA+DEAQAG+ASK+DVVLHAD SVS+ DNGRGIPTDLHP TKKS
Sbjct: 123 STGSRGLHHLVYEILDNAIDEAQAGYASKVDVVLHADGSVSVVDNGRGIPTDLHPATKKS 182

Query: 189 SLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYSR 248
           SLETVLTVLHAGGKFGG+SSGYSVSGGLHGVGLSVVNALSE+LEV +WRDG E  Q YSR
Sbjct: 183 SLETVLTVLHAGGKFGGTSSGYSVSGGLHGVGLSVVNALSEALEVSVWRDGMEHKQNYSR 242

Query: 249 GKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTI 308
           GKP TTLTC V   E K  +GT IRFWPDKEVFTT I+FD+NTI+GRIRELAFLNPK+TI
Sbjct: 243 GKPITTLTCRVLPLESKGTKGTSIRFWPDKEVFTTAIEFDHNTIAGRIRELAFLNPKVTI 302

Query: 309 TLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWCA 368
           +L KEDDD EK QY+EY +AGGL EYV+WLN+DK P+HDV GFR+E +G  +D+ALQWC+
Sbjct: 303 SLKKEDDDPEKTQYSEYSFAGGLTEYVSWLNTDKNPIHDVLGFRREINGATVDVALQWCS 362

Query: 369 DAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGL 428
           DAYSDT+LGYANSIRTIDGGTHI+G+KASLTRTLN L KKSK +K+KDISLSGEHVREGL
Sbjct: 363 DAYSDTMLGYANSIRTIDGGTHIEGVKASLTRTLNTLAKKSKTVKEKDISLSGEHVREGL 422

Query: 429 TCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNAL 488
           TC+VSV+V NPEFEGQTKTRLGNPEVRK+V+QS+QEYLTE+LELHPD+L+ I+SKSLNA 
Sbjct: 423 TCIVSVKVPNPEFEGQTKTRLGNPEVRKIVDQSVQEYLTEFLELHPDILESIISKSLNAY 482

Query: 489 KAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDRR 548
           KAALAAKRARELVR KSVL+SSSLPGKLADCSST+PE SEIFIVEGDSAGGSAKQGRDRR
Sbjct: 483 KAALAAKRARELVRSKSVLKSSSLPGKLADCSSTDPEVSEIFIVEGDSAGGSAKQGRDRR 542

Query: 549 FQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTDA 608
           FQAILPLRGKILNIERKDEAAMYKNEEIQNLIL LGLGVKGEDFKKE LRYHKIIILTDA
Sbjct: 543 FQAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFKKENLRYHKIIILTDA 602

Query: 609 DVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRSF 668
           DVDGAHIRTLLLTFFFRYQ+ALFD GCIY+GVPPL+KVERGK A YCYDD +LK++  +F
Sbjct: 603 DVDGAHIRTLLLTFFFRYQRALFDAGCIYVGVPPLFKVERGKNAQYCYDDADLKKITSNF 662

Query: 669 SSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDFR 728
            +NASYNIQRFKGLGEMMP QLWETTMNPE R+LKQL V+D AEAN+ FSSLMGARVD R
Sbjct: 663 PANASYNIQRFKGLGEMMPEQLWETTMNPETRILKQLVVDDIAEANMTFSSLMGARVDVR 722

Query: 729 KELIQKSANMINLGHLDI 740
           KELI+ +A  INL  LDI
Sbjct: 723 KELIKNAATRINLQRLDI 732

BLAST of CmaCh03G001390 vs. ExPASy Swiss-Prot
Match: Q9SS38 (DNA gyrase subunit B, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GYRBC PE=2 SV=3)

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 565/731 (77.29%), Postives = 629/731 (86.05%), Query Frame = 0

Query: 17  HLRLMAS-------SASLFPS-SIHLRSLTSSASRAPPFSFLEFSGTRFRARKIPTSLRL 76
           + RLMAS       S SL+PS   H  +L+SS  R            +F+   +  S RL
Sbjct: 14  YFRLMASRPRPRLFSHSLYPSLHRHSSALSSSTPR-----------IKFQLANV-FSQRL 73

Query: 77  AQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYIGSTGPRGL 136
            Q   VS  +FMSS+   ++   S +SK Y SE IQVLEGLDPVRKRPGMYIGSTG RGL
Sbjct: 74  VQRNAVSPKSFMSST--MESLQESSTSKDYSSEHIQVLEGLDPVRKRPGMYIGSTGSRGL 133

Query: 137 HHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKKSSLETVLT 196
           HHLVYEILDNA+DEAQAGFASKIDVVLH+D+SVSI+DNGRGIPTDLHP T KSSLETVLT
Sbjct: 134 HHLVYEILDNAIDEAQAGFASKIDVVLHSDDSVSISDNGRGIPTDLHPATGKSSLETVLT 193

Query: 197 VLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYSRGKPATTL 256
           VLHAGGKFGG SSGYSVSGGLHGVGLSVVNALSE+LEV + RDG EF QKYSRGKP TTL
Sbjct: 194 VLHAGGKFGGKSSGYSVSGGLHGVGLSVVNALSEALEVIVRRDGMEFQQKYSRGKPVTTL 253

Query: 257 TCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITLTKEDD 316
           TCHV   E +  QGT IRFWPDKEVFTT IQFD+NTI+GRIRELAFLNPK+TI+L KEDD
Sbjct: 254 TCHVLPPESRGTQGTCIRFWPDKEVFTTAIQFDHNTIAGRIRELAFLNPKVTISLKKEDD 313

Query: 317 DVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWCADAYSDTI 376
           D E++ Y+EY YAGGL EYV+WLN+DKKPLHDV GFRKE +G  +D++LQWC+DAYSDT+
Sbjct: 314 DPERDVYSEYFYAGGLTEYVSWLNTDKKPLHDVLGFRKEINGSTVDVSLQWCSDAYSDTM 373

Query: 377 LGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGLTCVVSVR 436
           LGYANSIRTIDGGTHI+G+KASLTRTLN+L KK K+IK+KDISLSGEHVREGLTC+VSV+
Sbjct: 374 LGYANSIRTIDGGTHIEGVKASLTRTLNSLAKKLKVIKEKDISLSGEHVREGLTCIVSVK 433

Query: 437 VQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNALKAALAAK 496
           V NPEFEGQTKTRLGNPEVRK+V+QS+QEYLTEYLELHPDVL+ I+SKSLNA KAALAAK
Sbjct: 434 VPNPEFEGQTKTRLGNPEVRKIVDQSVQEYLTEYLELHPDVLESIISKSLNAYKAALAAK 493

Query: 497 RARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDRRFQAILPL 556
           RARELVR KSVL+SSSLPGKLADCSST+P +SEIFIVEGDSAGGSAKQGRDRRFQAILPL
Sbjct: 494 RARELVRSKSVLKSSSLPGKLADCSSTDPAESEIFIVEGDSAGGSAKQGRDRRFQAILPL 553

Query: 557 RGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTDADVDGAHI 616
           RGKILNIERKDEAAMYKNEEIQNLIL LGLGVKGEDF KE LRYHKIIILTDADVDGAHI
Sbjct: 554 RGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFNKENLRYHKIIILTDADVDGAHI 613

Query: 617 RTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRSFSSNASYN 676
           RTLLLTFFFRYQ+ALFD GCIY+GVPPL+KVERGK+A+YCYDD  LK++  SF  NASYN
Sbjct: 614 RTLLLTFFFRYQRALFDAGCIYVGVPPLFKVERGKQAHYCYDDAALKKITASFPGNASYN 673

Query: 677 IQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDFRKELIQKS 736
           IQRFKGLGEMMP QLWETTMNP+ R+LKQL V+DAAE N+VFSSLMGARVD RKELI+ +
Sbjct: 674 IQRFKGLGEMMPAQLWETTMNPDTRILKQLVVDDAAETNMVFSSLMGARVDVRKELIKSA 730

Query: 737 ANMINLGHLDI 740
           A  +NL +LDI
Sbjct: 734 ATRMNLENLDI 730

BLAST of CmaCh03G001390 vs. ExPASy Swiss-Prot
Match: Q5NBJ3 (DNA gyrase subunit B, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=GYRB PE=2 SV=1)

HSP 1 Score: 1073.2 bits (2774), Expect = 1.5e-312
Identity = 541/738 (73.31%), Postives = 622/738 (84.28%), Query Frame = 0

Query: 9   LLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPP--FSFLEFSGTRFRAR-KIPTS 68
           L  +PP  HLRL+            LR L S+A+  P         S  R   R ++  +
Sbjct: 5   LRSSPPPRHLRLL------------LRRLLSTAAGRPSRLLPLPASSSARLLVRPRVAVA 64

Query: 69  LRLA----QNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYIG 128
              A    +   V+  AFM+S+AA++       +  Y +  +QVLE LD VR RPGMYIG
Sbjct: 65  AAAAGAPLRRNGVAVRAFMASTAASEAMQEKRVAGEYTAANVQVLEALDGVRTRPGMYIG 124

Query: 129 STGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKKS 188
           STG RGLHHLVYEILDNAVDEAQAG+A+K+DV+LH DNSVS+TDNGRGIPTD+HP TKKS
Sbjct: 125 STGSRGLHHLVYEILDNAVDEAQAGYATKVDVILHGDNSVSVTDNGRGIPTDIHPQTKKS 184

Query: 189 SLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYSR 248
            +ETVLT++HAGGKFGGS SGY+VSGGLHGVGLSVVNALSE+LEV +WRDGKE+ Q YSR
Sbjct: 185 CVETVLTLMHAGGKFGGSKSGYTVSGGLHGVGLSVVNALSEALEVTVWRDGKEYRQNYSR 244

Query: 249 GKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTI 308
           GK  T LT    S+E    QGTRIRFWPDK +FTT + FD+NTI+GRIRELAFLNP+LTI
Sbjct: 245 GKAITMLTSRTLSDESSSRQGTRIRFWPDKHIFTTTMDFDFNTIAGRIRELAFLNPELTI 304

Query: 309 TLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWCA 368
            LTKE+DD++  Q+NEYCYAGGLVEYV WLN+DKK LHD   FRKE DG+ +D++LQWC+
Sbjct: 305 ALTKEEDDLQV-QHNEYCYAGGLVEYVKWLNTDKKSLHDPIAFRKEMDGITVDVSLQWCS 364

Query: 369 DAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGL 428
           D+YSDT+LGYANSIRTIDGGTHIDG+K SLTRT+NN  KKSK +KDKDISLSGEHVREG+
Sbjct: 365 DSYSDTVLGYANSIRTIDGGTHIDGLKTSLTRTINNFAKKSKTLKDKDISLSGEHVREGM 424

Query: 429 TCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNAL 488
           TC+++V+V NPEFEGQTKTRLGNPEVR++VEQS+QE LTEYLELHPDVLD ILSKSLNAL
Sbjct: 425 TCIIAVKVPNPEFEGQTKTRLGNPEVRRIVEQSVQENLTEYLELHPDVLDSILSKSLNAL 484

Query: 489 KAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDRR 548
           KAALAAKRARELVR KSVL+SSSLPGKLADC+S++PE+SEIFIVEGDSAGGSAKQGRDR+
Sbjct: 485 KAALAAKRARELVRTKSVLKSSSLPGKLADCASSDPEESEIFIVEGDSAGGSAKQGRDRK 544

Query: 549 FQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTDA 608
           FQAILPLRGKILNIER+DEAA+YKNEEIQNLI+ALGLGVKGEDF KEALRYHKI+ILTDA
Sbjct: 545 FQAILPLRGKILNIERRDEAALYKNEEIQNLIVALGLGVKGEDFNKEALRYHKIVILTDA 604

Query: 609 DVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRSF 668
           DVDGAHIRTLLLTFFFRYQKALFDEGCIY+GVPPLYKVERGK+A+YCYDD +LK L  +F
Sbjct: 605 DVDGAHIRTLLLTFFFRYQKALFDEGCIYVGVPPLYKVERGKQAHYCYDDADLKELVNTF 664

Query: 669 SSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDFR 728
            +NASY+IQRFKGLGEMMP QLWETTM+PE+RMLKQL VEDAAEANVVFSSLMG RVD R
Sbjct: 665 PTNASYHIQRFKGLGEMMPAQLWETTMDPERRMLKQLKVEDAAEANVVFSSLMGTRVDVR 724

Query: 729 KELIQKSANMINLGHLDI 740
           K+LIQ +A+M+NL HLDI
Sbjct: 725 KQLIQNAASMVNLEHLDI 729

BLAST of CmaCh03G001390 vs. ExPASy Swiss-Prot
Match: Q5HK03 (DNA gyrase subunit B OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=gyrB PE=3 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 3.0e-187
Identity = 346/643 (53.81%), Postives = 452/643 (70.30%), Query Frame = 0

Query: 98  YGSEQIQVLEGLDPVRKRPGMYIGSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHA 157
           YG+ QIQVLEGL+ VRKRPGMYIGST  RGLHHLV+EI+DN++DEA AG+AS I+VV+  
Sbjct: 14  YGAGQIQVLEGLEAVRKRPGMYIGSTSERGLHHLVWEIVDNSIDEALAGYASHIEVVIEK 73

Query: 158 DNSVSITDNGRGIPTDLHPVTKKSSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVV 217
           DN + +TDNGRGIP D+     + ++E +LTVLHAGGKFGG   GY VSGGLHGVG SVV
Sbjct: 74  DNWIKVTDNGRGIPVDIQEKMGRPAVEVILTVLHAGGKFGG--GGYKVSGGLHGVGSSVV 133

Query: 218 NALSESLEVCIWRDGKEFHQKYSRGKPATTLTCHVPSNELK-----DHQGTRIRFWPDKE 277
           NALS+ LEV + R+G  +HQ Y +G         VP  +LK     D  GT IRF  DKE
Sbjct: 134 NALSQDLEVYVHRNGTIYHQAYKQG---------VPQFDLKEIGDTDKTGTAIRFKADKE 193

Query: 278 VFTTVIQFDYNTISGRIRELAFLNPKLTITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLN 337
           +FT    ++Y T+  RIRELAFLN  + ITL  +D+  E+ + + Y Y GG+  YV  LN
Sbjct: 194 IFTETTVYNYETLQKRIRELAFLNKGIQITL--KDEREEEVREDSYHYEGGIKSYVDLLN 253

Query: 338 SDKKPLHD-VFGFRKETDGVAIDIALQWCADAYSDTILGYANSIRTIDGGTHIDGMKASL 397
            +K+PLHD      +  D + ++IALQ+    Y+  +L YAN+I T +GGTH DG K +L
Sbjct: 254 ENKEPLHDEPIYIHQSKDDIEVEIALQY-NSGYATNLLTYANNIHTYEGGTHEDGFKRAL 313

Query: 398 TRTLNNLGKKSKIIKDKDISLSGEHVREGLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVV 457
           TR LN+ G +SKIIK+    LSGE  REGLT VVS++  +P+FEGQTKT+LGN EVR+VV
Sbjct: 314 TRVLNSYGTQSKIIKEDKDRLSGEDTREGLTAVVSIKHGDPQFEGQTKTKLGNSEVRQVV 373

Query: 458 EQSIQEYLTEYLELHPDVLDLILSKSLNALKAALAAKRARELVRQKSVLRSSSLPGKLAD 517
           ++   E+   +L  +P V  +I+ K + A +A +AAK+ARE+ R+KS L  SSLPGKLAD
Sbjct: 374 DRLFSEHFERFLYENPSVGRIIVEKGIMASRARVAAKKAREVTRRKSALDVSSLPGKLAD 433

Query: 518 CSSTNPEDSEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERKDEAAMYKNEEIQN 577
           CSS NPE+SEIF+VEGDSAGGS K GRD R QAILPLRGKILN+E+     +  N EI+ 
Sbjct: 434 CSSKNPEESEIFLVEGDSAGGSTKSGRDSRTQAILPLRGKILNVEKARLDRILNNNEIRQ 493

Query: 578 LILALGLGVKGEDFKKEALRYHKIIILTDADVDGAHIRTLLLTFFFRYQKALFDEGCIYI 637
           +I A G G+ GE F     RYHKI+I+TDADVDGAHIRTLLLTFF+R+ + L + G +YI
Sbjct: 494 MITAFGTGIGGE-FDISKARYHKIVIMTDADVDGAHIRTLLLTFFYRFMRPLIEAGYVYI 553

Query: 638 GVPPLYKVERGKKAYYCYDDKELKRLQRSFSSNASYNIQRFKGLGEMMPVQLWETTMNPE 697
             PPLYK+ +GK+ YY ++D+EL +L++  + +  ++I R+KGLGEM   QLWETTMNPE
Sbjct: 554 AQPPLYKLTQGKQKYYVFNDRELDKLKQELNPSPKWSIARYKGLGEMNADQLWETTMNPE 613

Query: 698 QRMLKQLAVEDAAEANVVFSSLMGARVDFRKELIQKSANMINL 735
            R + Q+ +EDA +A+  F  LMG  V+ R++ I+ +A   NL
Sbjct: 614 HRSMLQVRLEDAIDADQTFEMLMGDVVENRRQFIEDNAVYANL 641

BLAST of CmaCh03G001390 vs. ExPASy TrEMBL
Match: A0A6J1HVA6 (DNA gyrase subunit B OS=Cucurbita maxima OX=3661 GN=LOC111467169 PE=3 SV=1)

HSP 1 Score: 1450.6 bits (3754), Expect = 0.0e+00
Identity = 739/739 (100.00%), Postives = 739/739 (100.00%), Query Frame = 0

Query: 1   MALRNLLHLLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPPFSFLEFSGTRFRAR 60
           MALRNLLHLLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPPFSFLEFSGTRFRAR
Sbjct: 1   MALRNLLHLLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPPFSFLEFSGTRFRAR 60

Query: 61  KIPTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYI 120
           KIPTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYI
Sbjct: 61  KIPTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYI 120

Query: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKK 180
           GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKK
Sbjct: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKK 180

Query: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240
           SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS
Sbjct: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240

Query: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300
           RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT
Sbjct: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300

Query: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC 360
           ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC
Sbjct: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC 360

Query: 361 ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREG 420
           ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREG
Sbjct: 361 ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREG 420

Query: 421 LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA 480
           LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA
Sbjct: 421 LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA 480

Query: 481 LKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDR 540
           LKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDR
Sbjct: 481 LKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDR 540

Query: 541 RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD 600
           RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD
Sbjct: 541 RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD 600

Query: 601 ADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRS 660
           ADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRS
Sbjct: 601 ADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRS 660

Query: 661 FSSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDF 720
           FSSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDF
Sbjct: 661 FSSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDF 720

Query: 721 RKELIQKSANMINLGHLDI 740
           RKELIQKSANMINLGHLDI
Sbjct: 721 RKELIQKSANMINLGHLDI 739

BLAST of CmaCh03G001390 vs. ExPASy TrEMBL
Match: A0A6J1EXI8 (DNA gyrase subunit B OS=Cucurbita moschata OX=3662 GN=LOC111439391 PE=3 SV=1)

HSP 1 Score: 1415.6 bits (3663), Expect = 0.0e+00
Identity = 721/739 (97.56%), Postives = 729/739 (98.65%), Query Frame = 0

Query: 1   MALRNLLHLLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPPFSFLEFSGTRFRAR 60
           MALRNLL LLKTPPYLHLRLMASS SLFPSSIHLRSLTS+ASRAP FSFLEFSGTRFRAR
Sbjct: 1   MALRNLLLLLKTPPYLHLRLMASSTSLFPSSIHLRSLTSAASRAPSFSFLEFSGTRFRAR 60

Query: 61  KIPTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYI 120
           KIPTSLRLAQNVVVSA A MSSSAATKTSPGSES KAYGSEQIQVLEGLDPVRKRPGMYI
Sbjct: 61  KIPTSLRLAQNVVVSAKASMSSSAATKTSPGSESLKAYGSEQIQVLEGLDPVRKRPGMYI 120

Query: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKK 180
           GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHP TKK
Sbjct: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPATKK 180

Query: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240
           SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS
Sbjct: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240

Query: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300
           RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT
Sbjct: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300

Query: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC 360
           ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC
Sbjct: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC 360

Query: 361 ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREG 420
           ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGK+SKIIKDKDISLSGEHVREG
Sbjct: 361 ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKRSKIIKDKDISLSGEHVREG 420

Query: 421 LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA 480
           LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA
Sbjct: 421 LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA 480

Query: 481 LKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDR 540
           LKAALAAKRARELVRQKSVLRSSSLPGKLADCSST+PEDSEIFIVEGDSAGGSAKQGRDR
Sbjct: 481 LKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTDPEDSEIFIVEGDSAGGSAKQGRDR 540

Query: 541 RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD 600
           RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD
Sbjct: 541 RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD 600

Query: 601 ADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRS 660
           ADVDGAHIRTLLLTFFFRYQKALFD+GCIYIGVPPLYKVERGK+AYYCYDDKELKRLQ S
Sbjct: 601 ADVDGAHIRTLLLTFFFRYQKALFDQGCIYIGVPPLYKVERGKQAYYCYDDKELKRLQSS 660

Query: 661 FSSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDF 720
           F SNASYNIQRFKGLGEMMP QLWETTM+PEQRMLKQLA+EDAAEANVVFSSLMGARVDF
Sbjct: 661 FPSNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLAIEDAAEANVVFSSLMGARVDF 720

Query: 721 RKELIQKSANMINLGHLDI 740
           RKELIQKSANMI+LGHLDI
Sbjct: 721 RKELIQKSANMIDLGHLDI 739

BLAST of CmaCh03G001390 vs. ExPASy TrEMBL
Match: A0A5D3D861 (DNA gyrase subunit B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1676G00010 PE=3 SV=1)

HSP 1 Score: 1312.4 bits (3395), Expect = 0.0e+00
Identity = 675/741 (91.09%), Postives = 699/741 (94.33%), Query Frame = 0

Query: 1   MALRNLLHLLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPPFSFLEF--SGTRFR 60
           M LR L  LL+ PPYL LRLMASS SLFPSSIHLR L+SSAS AP FSFL+   SG RFR
Sbjct: 1   MTLRQL--LLRAPPYLPLRLMASSTSLFPSSIHLRFLSSSASIAPRFSFLQLRTSGGRFR 60

Query: 61  ARKIPTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGM 120
             KI TSLRL  NV VSA AFMSS+AAT+TS GSESSKAYGSEQIQVLEGLDPVRKRPGM
Sbjct: 61  TMKISTSLRLVPNVEVSAKAFMSSTAATETSTGSESSKAYGSEQIQVLEGLDPVRKRPGM 120

Query: 121 YIGSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVT 180
           YIGSTGPRGLHHLVYEILDNAVDEAQAGFAS+IDVVLHADNSVSITDNGRGIPTDLHPVT
Sbjct: 121 YIGSTGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPVT 180

Query: 181 KKSSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQK 240
           KKSSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEV IWRDGKEFHQK
Sbjct: 181 KKSSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVSIWRDGKEFHQK 240

Query: 241 YSRGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPK 300
           YSRGKPA+ LT HVPS+ELK+HQGTRIRFWPDKEVFTT IQFDYNTISGRIRELAFLNPK
Sbjct: 241 YSRGKPASILTSHVPSSELKNHQGTRIRFWPDKEVFTTGIQFDYNTISGRIRELAFLNPK 300

Query: 301 LTITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQ 360
           LTITLTKED+D EKNQYNEYCYAGGLVEYVAWLNSDK P+HDVFGFRKETDGVAID+ALQ
Sbjct: 301 LTITLTKEDNDREKNQYNEYCYAGGLVEYVAWLNSDKNPIHDVFGFRKETDGVAIDVALQ 360

Query: 361 WCADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVR 420
           WCADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSK  KDKDISLSGEHVR
Sbjct: 361 WCADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKTFKDKDISLSGEHVR 420

Query: 421 EGLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSL 480
           EGLTCVVSVRV+NPEFEGQTKTRLGNPEVRKVVEQSIQE+LTEYLELHPDVLD ILSKSL
Sbjct: 421 EGLTCVVSVRVRNPEFEGQTKTRLGNPEVRKVVEQSIQEHLTEYLELHPDVLDSILSKSL 480

Query: 481 NALKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGR 540
           NALKAALAAKRARELVRQKSVLRSSSLPGKLADCSS  PE+SEIFIVEGDSAGGSAKQGR
Sbjct: 481 NALKAALAAKRARELVRQKSVLRSSSLPGKLADCSSIKPEESEIFIVEGDSAGGSAKQGR 540

Query: 541 DRRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIIL 600
           DRRFQAILPLRGKILNIERKDEAAMYKNEEI NLI ALGLG KGEDFKKEALRYHKIIIL
Sbjct: 541 DRRFQAILPLRGKILNIERKDEAAMYKNEEINNLIRALGLGFKGEDFKKEALRYHKIIIL 600

Query: 601 TDADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQ 660
           TDADVDGAHIRTLLLTFFFRYQKALFDEGCIY+GVPPL+KVERGK+ YYCYDDKELK++Q
Sbjct: 601 TDADVDGAHIRTLLLTFFFRYQKALFDEGCIYVGVPPLFKVERGKQVYYCYDDKELKKVQ 660

Query: 661 RSFSSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARV 720
            SF SNASYNIQRFKGLGEMMP QLWETTM+PE+RMLKQLAVEDAAEANVVFSSLMGARV
Sbjct: 661 SSFPSNASYNIQRFKGLGEMMPAQLWETTMDPERRMLKQLAVEDAAEANVVFSSLMGARV 720

Query: 721 DFRKELIQKSANMINLGHLDI 740
           DFRKELIQKSA+ +NL HLDI
Sbjct: 721 DFRKELIQKSASTMNLEHLDI 739

BLAST of CmaCh03G001390 vs. ExPASy TrEMBL
Match: A0A1S3C142 (DNA gyrase subunit B OS=Cucumis melo OX=3656 GN=LOC103495821 PE=3 SV=1)

HSP 1 Score: 1310.8 bits (3391), Expect = 0.0e+00
Identity = 674/741 (90.96%), Postives = 699/741 (94.33%), Query Frame = 0

Query: 1   MALRNLLHLLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPPFSFLEF--SGTRFR 60
           M LR L  LL+ PPYL LRLMASS SLFPSSIHLR L+SSAS AP FSFL+   SG RFR
Sbjct: 1   MTLRQL--LLRAPPYLPLRLMASSTSLFPSSIHLRFLSSSASIAPRFSFLQLRTSGGRFR 60

Query: 61  ARKIPTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGM 120
             KI TSLRL  NV VSA AFMSS+AAT+TS GSESSKAYGSEQIQVLEGLDPVRKRPGM
Sbjct: 61  TMKISTSLRLVPNVEVSAKAFMSSTAATETSTGSESSKAYGSEQIQVLEGLDPVRKRPGM 120

Query: 121 YIGSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVT 180
           YIGSTGPRGLHHLVYEILDNAVDEAQAGFAS+IDVVLHADNSVSITDNGRGIPTDLHPVT
Sbjct: 121 YIGSTGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPVT 180

Query: 181 KKSSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQK 240
           KKSSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEV IWRDGKEFHQK
Sbjct: 181 KKSSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVSIWRDGKEFHQK 240

Query: 241 YSRGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPK 300
           YSRGKPA+ LT HVPS+ELK+HQGTRIRFWPDKEVFTT IQFDYNTISGRIRELAFLNPK
Sbjct: 241 YSRGKPASILTSHVPSSELKNHQGTRIRFWPDKEVFTTGIQFDYNTISGRIRELAFLNPK 300

Query: 301 LTITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQ 360
           LTITLTKED+D EKNQYNEYCYAGGLVEYVAWLNSDK P+HDVFGFRKETDGVAID+ALQ
Sbjct: 301 LTITLTKEDNDREKNQYNEYCYAGGLVEYVAWLNSDKNPIHDVFGFRKETDGVAIDVALQ 360

Query: 361 WCADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVR 420
           WCADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSK  KDKDISLSGEHVR
Sbjct: 361 WCADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKTFKDKDISLSGEHVR 420

Query: 421 EGLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSL 480
           EGLTCVVSVRV+NPEFEGQTKTRLGNPEVRKVVEQSIQE+LTEYLELHPDVLD ILSKSL
Sbjct: 421 EGLTCVVSVRVRNPEFEGQTKTRLGNPEVRKVVEQSIQEHLTEYLELHPDVLDSILSKSL 480

Query: 481 NALKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGR 540
           NALKAALAAKRARELVRQKSVLRSSSLPGKLADCSS  PE+SEIFIVEGDSAGGSAKQGR
Sbjct: 481 NALKAALAAKRARELVRQKSVLRSSSLPGKLADCSSIKPEESEIFIVEGDSAGGSAKQGR 540

Query: 541 DRRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIIL 600
           DRRFQAILPLRGKILNIERKDEAAMYKNEEI NLI ALGLG KGEDFKKEALRYHKIIIL
Sbjct: 541 DRRFQAILPLRGKILNIERKDEAAMYKNEEINNLIRALGLGFKGEDFKKEALRYHKIIIL 600

Query: 601 TDADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQ 660
           TDADVDGAHIRTLLLTFFFRYQKALF+EGCIY+GVPPL+KVERGK+ YYCYDDKELK++Q
Sbjct: 601 TDADVDGAHIRTLLLTFFFRYQKALFEEGCIYVGVPPLFKVERGKQVYYCYDDKELKKVQ 660

Query: 661 RSFSSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARV 720
            SF SNASYNIQRFKGLGEMMP QLWETTM+PE+RMLKQLAVEDAAEANVVFSSLMGARV
Sbjct: 661 SSFPSNASYNIQRFKGLGEMMPAQLWETTMDPERRMLKQLAVEDAAEANVVFSSLMGARV 720

Query: 721 DFRKELIQKSANMINLGHLDI 740
           DFRKELIQKSA+ +NL HLDI
Sbjct: 721 DFRKELIQKSASTMNLEHLDI 739

BLAST of CmaCh03G001390 vs. ExPASy TrEMBL
Match: A0A0A0L272 (DNA gyrase subunit B OS=Cucumis sativus OX=3659 GN=Csa_4G430890 PE=3 SV=1)

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 666/739 (90.12%), Postives = 694/739 (93.91%), Query Frame = 0

Query: 1   MALRNLLHLLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPPFSFLEFSGTRFRAR 60
           MALR L  LL+ PPYL LR MASS S FPSSIHLR L+SSAS AP FSFL+  G RFR  
Sbjct: 1   MALRQL--LLRAPPYLPLRFMASSTSFFPSSIHLRFLSSSASFAPRFSFLQLRGARFRTM 60

Query: 61  KIPTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYI 120
           KI TS+RL  N  VS+ AFMSSSAAT+TS  SESSKAYGSEQIQVLEGLDPVRKRPGMYI
Sbjct: 61  KISTSIRLVPNAEVSSKAFMSSSAATETSTRSESSKAYGSEQIQVLEGLDPVRKRPGMYI 120

Query: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKK 180
           GSTGPRGLHHLVYEILDNAVDEAQAGFAS+IDVVLHADNSVS+ DNGRGIPTDLHPVTKK
Sbjct: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSVADNGRGIPTDLHPVTKK 180

Query: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240
           SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEV IWRDG EFHQKYS
Sbjct: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVSIWRDGNEFHQKYS 240

Query: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300
           RGKPA+ LT HVPS+ELK+HQGT IRFWPDKEVFTT IQFDYNTISGRIRELAFLNPKLT
Sbjct: 241 RGKPASILTSHVPSSELKNHQGTHIRFWPDKEVFTTGIQFDYNTISGRIRELAFLNPKLT 300

Query: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC 360
           ITLTKED+D+EKNQYNEYCYAGGLVEYVAWLNSDK P+H+VFGFRKETDGVAID+ALQWC
Sbjct: 301 ITLTKEDNDLEKNQYNEYCYAGGLVEYVAWLNSDKNPIHEVFGFRKETDGVAIDVALQWC 360

Query: 361 ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREG 420
           ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKI KDKDISLSGEHVREG
Sbjct: 361 ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIFKDKDISLSGEHVREG 420

Query: 421 LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA 480
           LTCVVSVRV+NPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVL+LILSKSLNA
Sbjct: 421 LTCVVSVRVRNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLELILSKSLNA 480

Query: 481 LKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDR 540
           LKAALAAKRARELVRQKSVLRSSSLPGKLADCSS  PE+SEIFIVEGDSAGGSAKQGRDR
Sbjct: 481 LKAALAAKRARELVRQKSVLRSSSLPGKLADCSSIKPEESEIFIVEGDSAGGSAKQGRDR 540

Query: 541 RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD 600
           RFQAILPLRGKILNIERKDEAAMYKNEEI NLI ALGLG KGEDFKK+ALRYHKIIILTD
Sbjct: 541 RFQAILPLRGKILNIERKDEAAMYKNEEINNLIRALGLGFKGEDFKKDALRYHKIIILTD 600

Query: 601 ADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRS 660
           ADVDGAHIRTLLLTFFFRYQKALFDEGCIY+GVPPLYKVERGK+ YYCYDDKELK++Q S
Sbjct: 601 ADVDGAHIRTLLLTFFFRYQKALFDEGCIYVGVPPLYKVERGKQVYYCYDDKELKKVQSS 660

Query: 661 FSSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDF 720
           F SNASYNIQRFKGLGEMMP QLWETTM+PE+RMLKQLAVEDAAEANVVFSSLMG RVDF
Sbjct: 661 FPSNASYNIQRFKGLGEMMPAQLWETTMDPERRMLKQLAVEDAAEANVVFSSLMGVRVDF 720

Query: 721 RKELIQKSANMINLGHLDI 740
           RKELIQKSA+ +NL HLDI
Sbjct: 721 RKELIQKSASTMNLEHLDI 737

BLAST of CmaCh03G001390 vs. NCBI nr
Match: XP_022967735.1 (DNA gyrase subunit B, chloroplastic/mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 1450.6 bits (3754), Expect = 0.0e+00
Identity = 739/739 (100.00%), Postives = 739/739 (100.00%), Query Frame = 0

Query: 1   MALRNLLHLLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPPFSFLEFSGTRFRAR 60
           MALRNLLHLLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPPFSFLEFSGTRFRAR
Sbjct: 1   MALRNLLHLLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPPFSFLEFSGTRFRAR 60

Query: 61  KIPTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYI 120
           KIPTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYI
Sbjct: 61  KIPTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYI 120

Query: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKK 180
           GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKK
Sbjct: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKK 180

Query: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240
           SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS
Sbjct: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240

Query: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300
           RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT
Sbjct: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300

Query: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC 360
           ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC
Sbjct: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC 360

Query: 361 ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREG 420
           ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREG
Sbjct: 361 ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREG 420

Query: 421 LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA 480
           LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA
Sbjct: 421 LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA 480

Query: 481 LKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDR 540
           LKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDR
Sbjct: 481 LKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDR 540

Query: 541 RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD 600
           RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD
Sbjct: 541 RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD 600

Query: 601 ADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRS 660
           ADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRS
Sbjct: 601 ADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRS 660

Query: 661 FSSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDF 720
           FSSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDF
Sbjct: 661 FSSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDF 720

Query: 721 RKELIQKSANMINLGHLDI 740
           RKELIQKSANMINLGHLDI
Sbjct: 721 RKELIQKSANMINLGHLDI 739

BLAST of CmaCh03G001390 vs. NCBI nr
Match: KAG6603309.1 (DNA gyrase subunit B, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1416.4 bits (3665), Expect = 0.0e+00
Identity = 721/739 (97.56%), Postives = 730/739 (98.78%), Query Frame = 0

Query: 1   MALRNLLHLLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPPFSFLEFSGTRFRAR 60
           MALRNLL LLKTPPYLHLRLMASS SLFPSSIHLRSLTS+ASRAP FSFLEFSGTRFRAR
Sbjct: 1   MALRNLLLLLKTPPYLHLRLMASSTSLFPSSIHLRSLTSAASRAPSFSFLEFSGTRFRAR 60

Query: 61  KIPTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYI 120
           KIPTSLRLAQNVVVSA A MSS+AATKTSPGSES KAYGSEQIQVLEGLDPVRKRPGMYI
Sbjct: 61  KIPTSLRLAQNVVVSAKASMSSTAATKTSPGSESLKAYGSEQIQVLEGLDPVRKRPGMYI 120

Query: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKK 180
           GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKK
Sbjct: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKK 180

Query: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240
           SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS
Sbjct: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240

Query: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300
           RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT
Sbjct: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300

Query: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC 360
           ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC
Sbjct: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC 360

Query: 361 ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREG 420
           ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGK+SKIIKDKDISLSGEHVREG
Sbjct: 361 ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKRSKIIKDKDISLSGEHVREG 420

Query: 421 LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA 480
           LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA
Sbjct: 421 LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA 480

Query: 481 LKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDR 540
           LKAALAAKRARELVRQKSVLRSSSLPGKLADCSST+PEDSEIFIVEGDSAGGSAKQGRDR
Sbjct: 481 LKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTDPEDSEIFIVEGDSAGGSAKQGRDR 540

Query: 541 RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD 600
           RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD
Sbjct: 541 RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD 600

Query: 601 ADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRS 660
           ADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGK+AYYCYD+KELKRLQ S
Sbjct: 601 ADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKQAYYCYDEKELKRLQSS 660

Query: 661 FSSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDF 720
           F SNA+YNIQRFKGLGEMMP QLWETTM+PEQRMLKQLA+EDAAEANVVFSSLMGARVDF
Sbjct: 661 FPSNATYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLAIEDAAEANVVFSSLMGARVDF 720

Query: 721 RKELIQKSANMINLGHLDI 740
           RKELIQKSANMINLGHLDI
Sbjct: 721 RKELIQKSANMINLGHLDI 739

BLAST of CmaCh03G001390 vs. NCBI nr
Match: XP_023544528.1 (DNA gyrase subunit B, chloroplastic/mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1416.4 bits (3665), Expect = 0.0e+00
Identity = 722/739 (97.70%), Postives = 728/739 (98.51%), Query Frame = 0

Query: 1   MALRNLLHLLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPPFSFLEFSGTRFRAR 60
           MALRNLL LLKTPPYLHLRLMASS SLFPSSIHLRSLTS+ASRAP FSFLEFSGTRFRAR
Sbjct: 1   MALRNLLLLLKTPPYLHLRLMASSTSLFPSSIHLRSLTSAASRAPSFSFLEFSGTRFRAR 60

Query: 61  KIPTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYI 120
           KIPTSLRLAQNVVVSA A MSSSAATKTSPGSES KAYGSEQIQVLEGLDPVRKRPGMYI
Sbjct: 61  KIPTSLRLAQNVVVSAKASMSSSAATKTSPGSESLKAYGSEQIQVLEGLDPVRKRPGMYI 120

Query: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKK 180
           GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHP TKK
Sbjct: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPATKK 180

Query: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240
           SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS
Sbjct: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240

Query: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300
           RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT
Sbjct: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300

Query: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC 360
           ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC
Sbjct: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC 360

Query: 361 ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREG 420
           ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREG
Sbjct: 361 ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREG 420

Query: 421 LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA 480
           LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA
Sbjct: 421 LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA 480

Query: 481 LKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDR 540
           LKAALAAKRARELVRQKSVLRSSSLPGKLADCSST+PEDSEIFIVEGDSAGGSAKQGRDR
Sbjct: 481 LKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTDPEDSEIFIVEGDSAGGSAKQGRDR 540

Query: 541 RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD 600
           RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD
Sbjct: 541 RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD 600

Query: 601 ADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRS 660
           ADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGK+AYYCYDDKELKRLQ S
Sbjct: 601 ADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKQAYYCYDDKELKRLQSS 660

Query: 661 FSSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDF 720
           F SNASYNIQRFKGLGEMMP QLWETTM+PEQRMLKQLA+EDAAEANVVFSSLMG RVDF
Sbjct: 661 FPSNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLAIEDAAEANVVFSSLMGVRVDF 720

Query: 721 RKELIQKSANMINLGHLDI 740
           RKELIQKSANMI+LGHLDI
Sbjct: 721 RKELIQKSANMIDLGHLDI 739

BLAST of CmaCh03G001390 vs. NCBI nr
Match: XP_022932851.1 (DNA gyrase subunit B, chloroplastic/mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 1415.6 bits (3663), Expect = 0.0e+00
Identity = 721/739 (97.56%), Postives = 729/739 (98.65%), Query Frame = 0

Query: 1   MALRNLLHLLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPPFSFLEFSGTRFRAR 60
           MALRNLL LLKTPPYLHLRLMASS SLFPSSIHLRSLTS+ASRAP FSFLEFSGTRFRAR
Sbjct: 1   MALRNLLLLLKTPPYLHLRLMASSTSLFPSSIHLRSLTSAASRAPSFSFLEFSGTRFRAR 60

Query: 61  KIPTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYI 120
           KIPTSLRLAQNVVVSA A MSSSAATKTSPGSES KAYGSEQIQVLEGLDPVRKRPGMYI
Sbjct: 61  KIPTSLRLAQNVVVSAKASMSSSAATKTSPGSESLKAYGSEQIQVLEGLDPVRKRPGMYI 120

Query: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKK 180
           GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHP TKK
Sbjct: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPATKK 180

Query: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240
           SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS
Sbjct: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240

Query: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300
           RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT
Sbjct: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300

Query: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC 360
           ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC
Sbjct: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWC 360

Query: 361 ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREG 420
           ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGK+SKIIKDKDISLSGEHVREG
Sbjct: 361 ADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKRSKIIKDKDISLSGEHVREG 420

Query: 421 LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA 480
           LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA
Sbjct: 421 LTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNA 480

Query: 481 LKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDR 540
           LKAALAAKRARELVRQKSVLRSSSLPGKLADCSST+PEDSEIFIVEGDSAGGSAKQGRDR
Sbjct: 481 LKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTDPEDSEIFIVEGDSAGGSAKQGRDR 540

Query: 541 RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD 600
           RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD
Sbjct: 541 RFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTD 600

Query: 601 ADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRS 660
           ADVDGAHIRTLLLTFFFRYQKALFD+GCIYIGVPPLYKVERGK+AYYCYDDKELKRLQ S
Sbjct: 601 ADVDGAHIRTLLLTFFFRYQKALFDQGCIYIGVPPLYKVERGKQAYYCYDDKELKRLQSS 660

Query: 661 FSSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDF 720
           F SNASYNIQRFKGLGEMMP QLWETTM+PEQRMLKQLA+EDAAEANVVFSSLMGARVDF
Sbjct: 661 FPSNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLAIEDAAEANVVFSSLMGARVDF 720

Query: 721 RKELIQKSANMINLGHLDI 740
           RKELIQKSANMI+LGHLDI
Sbjct: 721 RKELIQKSANMIDLGHLDI 739

BLAST of CmaCh03G001390 vs. NCBI nr
Match: XP_038882822.1 (DNA gyrase subunit B, chloroplastic/mitochondrial-like [Benincasa hispida])

HSP 1 Score: 1328.9 bits (3438), Expect = 0.0e+00
Identity = 683/740 (92.30%), Postives = 703/740 (95.00%), Query Frame = 0

Query: 1   MALRNLLHLLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPPFSFLEFSGTRFRAR 60
           MALR   HLLK P YL LRLMASS SLFPSSIHLRSL+SSAS AP F+FL+ SGTRFR R
Sbjct: 1   MALR---HLLKAPGYLPLRLMASSTSLFPSSIHLRSLSSSASLAPSFTFLQSSGTRFRTR 60

Query: 61  KIPTSLRLAQNVVVSATAFMSSSAA-TKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMY 120
           KI TS+RL QNV     AFMSSSAA T+TS  SES KAYGSEQIQVLEGLDPVRKRPGMY
Sbjct: 61  KISTSIRLVQNV-----AFMSSSAATTETSTASESLKAYGSEQIQVLEGLDPVRKRPGMY 120

Query: 121 IGSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTK 180
           IGSTGPRGLHHLVYEILDNAVDEAQAGFAS+IDVVLHADNSVSITDNGRGIPTDLHPVTK
Sbjct: 121 IGSTGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPVTK 180

Query: 181 KSSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKY 240
           KSSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSE LEV +WRDGKEFHQKY
Sbjct: 181 KSSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVSVWRDGKEFHQKY 240

Query: 241 SRGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKL 300
           SRGKPAT L CHVPS+ELK+HQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKL
Sbjct: 241 SRGKPATILACHVPSSELKNHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKL 300

Query: 301 TITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQW 360
           TITLTKED+D++ NQYNEYCYAGGLVEYVAWLNSDKKPLHDVF FRKETDGVAID ALQW
Sbjct: 301 TITLTKEDNDLKNNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFAFRKETDGVAIDAALQW 360

Query: 361 CADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVRE 420
           CADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKI KDKDISLSGEHVRE
Sbjct: 361 CADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIFKDKDISLSGEHVRE 420

Query: 421 GLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLN 480
           GLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLN
Sbjct: 421 GLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLN 480

Query: 481 ALKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRD 540
           ALKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPE+SEIFIVEGDSAGGSAKQGRD
Sbjct: 481 ALKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEESEIFIVEGDSAGGSAKQGRD 540

Query: 541 RRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILT 600
           RRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILT
Sbjct: 541 RRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILT 600

Query: 601 DADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQR 660
           DADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGK+ +YCYDDKELK+LQ 
Sbjct: 601 DADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKQVHYCYDDKELKKLQS 660

Query: 661 SFSSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVD 720
           SF SNASYNIQRFKGLGEMMP QLWETTM+PEQRMLKQLA+E+ A+ANVVFSSLMGARVD
Sbjct: 661 SFPSNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLAIEEEADANVVFSSLMGARVD 720

Query: 721 FRKELIQKSANMINLGHLDI 740
           FRKELIQKSANMIN  HLDI
Sbjct: 721 FRKELIQKSANMINFEHLDI 732

BLAST of CmaCh03G001390 vs. TAIR 10
Match: AT5G04130.1 (DNA GYRASE B2 )

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 565/738 (76.56%), Postives = 630/738 (85.37%), Query Frame = 0

Query: 9   LLKTPPYL---HLRLMASSASLFPSS----IHLRSLTSSASRAPPFSFLEFSGTRFRARK 68
           LL+   YL   +LRLM S   LF SS    +H  S T S   +PPFS        FR + 
Sbjct: 3   LLQRASYLRLYYLRLMGSRPRLFSSSLSPALHRHSSTLS---SPPFS---SPSPSFRLKF 62

Query: 69  IPTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYIG 128
             TS+ L+Q  ++   A  S   +T+ S  + +SK Y SEQIQVLEGLDPVRKRPGMYIG
Sbjct: 63  QLTSV-LSQR-LIQRNAISSRFLSTEASQETTTSKGYSSEQIQVLEGLDPVRKRPGMYIG 122

Query: 129 STGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKKS 188
           STG RGLHHLVYEILDNA+DEAQAG+ASK+DVVLHAD SVS+ DNGRGIPTDLHP TKKS
Sbjct: 123 STGSRGLHHLVYEILDNAIDEAQAGYASKVDVVLHADGSVSVVDNGRGIPTDLHPATKKS 182

Query: 189 SLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYSR 248
           SLETVLTVLHAGGKFGG+SSGYSVSGGLHGVGLSVVNALSE+LEV +WRDG E  Q YSR
Sbjct: 183 SLETVLTVLHAGGKFGGTSSGYSVSGGLHGVGLSVVNALSEALEVSVWRDGMEHKQNYSR 242

Query: 249 GKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTI 308
           GKP TTLTC V   E K  +GT IRFWPDKEVFTT I+FD+NTI+GRIRELAFLNPK+TI
Sbjct: 243 GKPITTLTCRVLPLESKGTKGTSIRFWPDKEVFTTAIEFDHNTIAGRIRELAFLNPKVTI 302

Query: 309 TLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWCA 368
           +L KEDDD EK QY+EY +AGGL EYV+WLN+DK P+HDV GFR+E +G  +D+ALQWC+
Sbjct: 303 SLKKEDDDPEKTQYSEYSFAGGLTEYVSWLNTDKNPIHDVLGFRREINGATVDVALQWCS 362

Query: 369 DAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGL 428
           DAYSDT+LGYANSIRTIDGGTHI+G+KASLTRTLN L KKSK +K+KDISLSGEHVREGL
Sbjct: 363 DAYSDTMLGYANSIRTIDGGTHIEGVKASLTRTLNTLAKKSKTVKEKDISLSGEHVREGL 422

Query: 429 TCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNAL 488
           TC+VSV+V NPEFEGQTKTRLGNPEVRK+V+QS+QEYLTE+LELHPD+L+ I+SKSLNA 
Sbjct: 423 TCIVSVKVPNPEFEGQTKTRLGNPEVRKIVDQSVQEYLTEFLELHPDILESIISKSLNAY 482

Query: 489 KAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDRR 548
           KAALAAKRARELVR KSVL+SSSLPGKLADCSST+PE SEIFIVEGDSAGGSAKQGRDRR
Sbjct: 483 KAALAAKRARELVRSKSVLKSSSLPGKLADCSSTDPEVSEIFIVEGDSAGGSAKQGRDRR 542

Query: 549 FQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTDA 608
           FQAILPLRGKILNIERKDEAAMYKNEEIQNLIL LGLGVKGEDFKKE LRYHKIIILTDA
Sbjct: 543 FQAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFKKENLRYHKIIILTDA 602

Query: 609 DVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRSF 668
           DVDGAHIRTLLLTFFFRYQ+ALFD GCIY+GVPPL+KVERGK A YCYDD +LK++  +F
Sbjct: 603 DVDGAHIRTLLLTFFFRYQRALFDAGCIYVGVPPLFKVERGKNAQYCYDDADLKKITSNF 662

Query: 669 SSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDFR 728
            +NASYNIQRFKGLGEMMP QLWETTMNPE R+LKQL V+D AEAN+ FSSLMGARVD R
Sbjct: 663 PANASYNIQRFKGLGEMMPEQLWETTMNPETRILKQLVVDDIAEANMTFSSLMGARVDVR 722

Query: 729 KELIQKSANMINLGHLDI 740
           KELI+ +A  INL  LDI
Sbjct: 723 KELIKNAATRINLQRLDI 732

BLAST of CmaCh03G001390 vs. TAIR 10
Match: AT3G10270.1 (DNA GYRASE B1 )

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 565/763 (74.05%), Postives = 629/763 (82.44%), Query Frame = 0

Query: 17  HLRLMAS-------SASLFPS-SIHLRSLTSSASRAPPFSFLEFSGTRFRARKIPTSLRL 76
           + RLMAS       S SL+PS   H  +L+SS  R            +F+   +  S RL
Sbjct: 24  YFRLMASRPRPRLFSHSLYPSLHRHSSALSSSTPR-----------IKFQLANV-FSQRL 83

Query: 77  AQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYIGSTGPRGL 136
            Q   VS  +FMSS+   ++   S +SK Y SE IQVLEGLDPVRKRPGMYIGSTG RGL
Sbjct: 84  VQRNAVSPKSFMSST--MESLQESSTSKDYSSEHIQVLEGLDPVRKRPGMYIGSTGSRGL 143

Query: 137 HHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKKSSLETVLT 196
           HHLVYEILDNA+DEAQAGFASKIDVVLH+D+SVSI+DNGRGIPTDLHP T KSSLETVLT
Sbjct: 144 HHLVYEILDNAIDEAQAGFASKIDVVLHSDDSVSISDNGRGIPTDLHPATGKSSLETVLT 203

Query: 197 VLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYSRGKPATTL 256
           VLHAGGKFGG SSGYSVSGGLHGVGLSVVNALSE+LEV + RDG EF QKYSRGKP TTL
Sbjct: 204 VLHAGGKFGGKSSGYSVSGGLHGVGLSVVNALSEALEVIVRRDGMEFQQKYSRGKPVTTL 263

Query: 257 TCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITLTKEDD 316
           TCHV   E +  QGT IRFWPDKEVFTT IQFD+NTI+GRIRELAFLNPK+TI+L KEDD
Sbjct: 264 TCHVLPPESRGTQGTCIRFWPDKEVFTTAIQFDHNTIAGRIRELAFLNPKVTISLKKEDD 323

Query: 317 DVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWCADAYSDTI 376
           D E++ Y+EY YAGGL EYV+WLN+DKKPLHDV GFRKE +G  +D++LQWC+DAYSDT+
Sbjct: 324 DPERDVYSEYFYAGGLTEYVSWLNTDKKPLHDVLGFRKEINGSTVDVSLQWCSDAYSDTM 383

Query: 377 LGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIK---------------------- 436
           LGYANSIRTIDGGTHI+G+KASLTRTLN+L KK K+IK                      
Sbjct: 384 LGYANSIRTIDGGTHIEGVKASLTRTLNSLAKKLKVIKVKGYIIYSSPWIETKFKYSSAL 443

Query: 437 ----------DKDISLSGEHVREGLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQ 496
                     +KDISLSGEHVREGLTC+VSV+V NPEFEGQTKTRLGNPEVRK+V+QS+Q
Sbjct: 444 KRHALRTINNEKDISLSGEHVREGLTCIVSVKVPNPEFEGQTKTRLGNPEVRKIVDQSVQ 503

Query: 497 EYLTEYLELHPDVLDLILSKSLNALKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTN 556
           EYLTEYLELHPDVL+ I+SKSLNA KAALAAKRARELVR KSVL+SSSLPGKLADCSST+
Sbjct: 504 EYLTEYLELHPDVLESIISKSLNAYKAALAAKRARELVRSKSVLKSSSLPGKLADCSSTD 563

Query: 557 PEDSEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILAL 616
           P +SEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERKDEAAMYKNEEIQNLIL L
Sbjct: 564 PAESEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILGL 623

Query: 617 GLGVKGEDFKKEALRYHKIIILTDADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPL 676
           GLGVKGEDF KE LRYHKIIILTDADVDGAHIRTLLLTFFFRYQ+ALFD GCIY+GVPPL
Sbjct: 624 GLGVKGEDFNKENLRYHKIIILTDADVDGAHIRTLLLTFFFRYQRALFDAGCIYVGVPPL 683

Query: 677 YKVERGKKAYYCYDDKELKRLQRSFSSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLK 736
           +KVERGK+A+YCYDD  LK++  SF  NASYNIQRFKGLGEMMP QLWETTMNP+ R+LK
Sbjct: 684 FKVERGKQAHYCYDDAALKKITASFPGNASYNIQRFKGLGEMMPAQLWETTMNPDTRILK 743

Query: 737 QLAVEDAAEANVVFSSLMGARVDFRKELIQKSANMINLGHLDI 740
           QL V+DAAE N+VFSSLMGARVD RKELI+ +A  +NL +LDI
Sbjct: 744 QLVVDDAAETNMVFSSLMGARVDVRKELIKSAATRMNLENLDI 772

BLAST of CmaCh03G001390 vs. TAIR 10
Match: AT5G04130.2 (DNA GYRASE B2 )

HSP 1 Score: 728.4 bits (1879), Expect = 5.8e-210
Identity = 379/522 (72.61%), Postives = 433/522 (82.95%), Query Frame = 0

Query: 9   LLKTPPYL---HLRLMASSASLFPSS----IHLRSLTSSASRAPPFSFLEFSGTRFRARK 68
           LL+   YL   +LRLM S   LF SS    +H  S T S   +PPFS        FR + 
Sbjct: 3   LLQRASYLRLYYLRLMGSRPRLFSSSLSPALHRHSSTLS---SPPFS---SPSPSFRLKF 62

Query: 69  IPTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYIG 128
             TS+ L+Q  ++   A  S   +T+ S  + +SK Y SEQIQVLEGLDPVRKRPGMYIG
Sbjct: 63  QLTSV-LSQR-LIQRNAISSRFLSTEASQETTTSKGYSSEQIQVLEGLDPVRKRPGMYIG 122

Query: 129 STGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKKS 188
           STG RGLHHLVYEILDNA+DEAQAG+ASK+DVVLHAD SVS+ DNGRGIPTDLHP TKKS
Sbjct: 123 STGSRGLHHLVYEILDNAIDEAQAGYASKVDVVLHADGSVSVVDNGRGIPTDLHPATKKS 182

Query: 189 SLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYSR 248
           SLETVLTVLHAGGKFGG+SSGYSVSGGLHGVGLSVVNALSE+LEV +WRDG E  Q YSR
Sbjct: 183 SLETVLTVLHAGGKFGGTSSGYSVSGGLHGVGLSVVNALSEALEVSVWRDGMEHKQNYSR 242

Query: 249 GKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTI 308
           GKP TTLTC V   E K  +GT IRFWPDKEVFTT I+FD+NTI+GRIRELAFLNPK+TI
Sbjct: 243 GKPITTLTCRVLPLESKGTKGTSIRFWPDKEVFTTAIEFDHNTIAGRIRELAFLNPKVTI 302

Query: 309 TLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAIDIALQWCA 368
           +L KEDDD EK QY+EY +AGGL EYV+WLN+DK P+HDV GFR+E +G  +D+ALQWC+
Sbjct: 303 SLKKEDDDPEKTQYSEYSFAGGLTEYVSWLNTDKNPIHDVLGFRREINGATVDVALQWCS 362

Query: 369 DAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGL 428
           DAYSDT+LGYANSIRTIDGGTHI+G+KASLTRTLN L KKSK +K+KDISLSGEHVREGL
Sbjct: 363 DAYSDTMLGYANSIRTIDGGTHIEGVKASLTRTLNTLAKKSKTVKEKDISLSGEHVREGL 422

Query: 429 TCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNAL 488
           TC+VSV+V NPEFEGQTKTRLGNPEVRK+V+QS+QEYLTE+LELHPD+L+ I+SKSLNA 
Sbjct: 423 TCIVSVKVPNPEFEGQTKTRLGNPEVRKIVDQSVQEYLTEFLELHPDILESIISKSLNAY 482

Query: 489 KAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIF 524
           KAALAAKRARELVR KSVL+SSSLPGKLADCSST+PE S ++
Sbjct: 483 KAALAAKRARELVRSKSVLKSSSLPGKLADCSSTDPEVSGMY 516

BLAST of CmaCh03G001390 vs. TAIR 10
Match: AT5G04110.1 (DNA GYRASE B3 )

HSP 1 Score: 275.4 bits (703), Expect = 1.3e-73
Identity = 148/252 (58.73%), Postives = 184/252 (73.02%), Query Frame = 0

Query: 295 LNPKLTITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETDGVAID 354
           L  ++T++L  ED+D  K    EY YA GL E+V WLN+DKKPLHDV GFRKE +G  I+
Sbjct: 17  LRVQITVSLEMEDNDPNK----EYLYAKGLSEFVTWLNADKKPLHDVLGFRKEINGTTIN 76

Query: 355 IALQWCADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSG 414
           IALQWC D YS+ ILGYAN IRT+DGGT+IDG+KAS+TRTLN+L +KSK+++DKDI  + 
Sbjct: 77  IALQWCVDGYSNKILGYANGIRTMDGGTYIDGVKASITRTLNSLVEKSKLVEDKDIIFTE 136

Query: 415 EHVREGLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLIL 474
           EHV EGLTC+VSV V  PEFEGQT+ RLGNP VR++V+QS+QE L E  ELHPDV + I+
Sbjct: 137 EHVMEGLTCIVSVIVPKPEFEGQTQ-RLGNPNVREIVDQSVQECLMESFELHPDVFESIM 196

Query: 475 SKSLNALKAALAAKRARELVRQKS---VLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAG 534
           SKS NA K  LA KRAR++   +S   V    S+P KL + SS   E SE FI  G S+G
Sbjct: 197 SKSYNAYKTDLAVKRARDVYSSESVAMVCAIESIPMKLTNSSS---ETSETFIGRGVSSG 256

Query: 535 GSAKQGRDRRFQ 544
           G+AK   DR F+
Sbjct: 257 GAAKHDSDRCFK 260

BLAST of CmaCh03G001390 vs. TAIR 10
Match: AT3G23890.1 (topoisomerase II )

HSP 1 Score: 132.1 bits (331), Expect = 1.8e-30
Identity = 156/640 (24.38%), Postives = 268/640 (41.88%), Query Frame = 0

Query: 81  SSSAATKTSPGSESSKAYGSEQIQVL----EGLDPVRKRPGMYIGS-------------- 140
           +S+AA      ++S  A G + I+ +      L+ +  RP  YIGS              
Sbjct: 9   NSNAANVAKAPAKSRAAAGGKTIEEMYQKKSQLEHILLRPDTYIGSIEKHTQTLWVYEKD 68

Query: 141 -------TGPRGLHHLVYEILDNAVDEAQA-GFASKIDVVLHAD-NSVSITDNGRGIPTD 200
                  T   GL+ +  EIL NA D  Q       + VV+  + N +S+ ++G G+P +
Sbjct: 69  EMVQRPVTYVPGLYKIFDEILVNAADNKQRDAKMDSVQVVIDVEQNLISVCNSGAGVPVE 128

Query: 201 LHPVTKKSSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSES--LEVCIWRD 260
           +H        E +   L     +  +      +GG +G G  + N  S    +E    + 
Sbjct: 129 IHQEEGIYVPEMIFGHLLTSSNYDDNVK--KTTGGRNGYGAKLTNIFSTEFIIETADGKR 188

Query: 261 GKEFHQKYSRGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFT-TVIQFDYNTI-SGRI 320
            K++ Q +       +       N  K    T++ F PD + F  T ++ D   + S R+
Sbjct: 189 LKKYKQVFENNMGKKSEPVITKCN--KSENWTKVTFKPDLKKFNMTELEDDVVALMSKRV 248

Query: 321 RELAFLNPKLTITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHDVFGFRKETD 380
            ++A    K ++ +      +    + +Y     +  Y++  N  K    D      E  
Sbjct: 249 FDIAGCLGK-SVKVELNGKQIPVKSFTDY-----VDLYLSAAN--KSRTEDPLPRLTEKV 308

Query: 381 GVAIDIALQWCADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKD 440
               ++ +      +    + + NSI TI GGTH+D + + +T  +     K    K+K+
Sbjct: 309 NDRWEVCVSLSEGQFQQ--VSFVNSIATIKGGTHVDYVTSQITNHIVAAVNK----KNKN 368

Query: 441 ISLSGEHVREGLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLEL--HP 500
            ++   +V+  L   V+  + NP F+ QTK  L    +R+    S  E   ++L+     
Sbjct: 369 ANVKAHNVKNHLWVFVNALIDNPAFDSQTKETL---TLRQSSFGSKCELSEDFLKKVGKS 428

Query: 501 DVLDLILS----KSLNALKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIF 560
            V++ +LS    K    LK +  AK  R LV +             A+    N     + 
Sbjct: 429 GVVENLLSWADFKQNKDLKKSDGAKTGRVLVEKLD---------DAAEAGGKNSRLCTLI 488

Query: 561 IVEGDSAGGSAKQGRD---RRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGV 620
           + EGDSA   A  GR      +  + PLRGK+LN+       +  N+EI+NL   LGL  
Sbjct: 489 LTEGDSAKSLALAGRSVLGNNYCGVFPLRGKLLNVREASTTQITNNKEIENLKKILGLKQ 548

Query: 621 KGEDFKKEALRYHKIIILTDADVDGAHIRTLLLTFFFRYQKALFDEGCIYIG-VPPLYKV 677
             +     +LRY +++I+TD D DG+HI+ LL+ F   +  +L       +  + P+ K 
Sbjct: 549 NMKYENVNSLRYGQMMIMTDQDHDGSHIKGLLINFIHSFWPSLLQVPSFLVEFITPIVKA 608