CmaCh02G016540 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh02G016540
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionAmino acid permease family protein
LocationCma_Chr02: 9373616 .. 9376296 (-)
RNA-Seq ExpressionCmaCh02G016540
SyntenyCmaCh02G016540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAGAATTTCCTCCTCCTCCTTCTTCTTCTTCTGTAAGTTAATGTTAGATTCTGAAACACCATACTGATTTGAGGGCTTTTCGGCGGTAGTTTTCTCCGTTTGTTCGACGGCGGAGGTGCTATTGCAGTAATGCATCCTTCGACGCTGGCGATTGATTTTTCTTGATGGTTTGTTCTTTCTTCTACTTGATTTTGATTCTCCTTTTGTTCGTTTGTTCTTGCATATTTCAGTTTTACGATCCTGAAGATTTTTCTCCAAGATTACGTTTATTTTTATTTTAATATCTCGATTTTTGGTTTTCTGAATTGTTTGTTCTTCTTTCTTTTTCTTTCAGCTTAATATTTAATTTCGAATCTTATTTTGTGTTTTGTTCGTCTTTTTTGGGACTCTCGTTTCATTTGTAATGTGAAAACAGTGTCTAGGTGTAGTTCTGTCATATTTGATTAATGCGAATCATGAGTTTGATGCGTCTTTGGAGACGTTAATCAAGGAATTACTTGCTATGGCAAGATGTAAGATGTCGATTGGTACTTACTGGATTTACGAAACTGAAAGTGTTTATGAACTGATAGGAATTAAGTGACCGAGAGTAGTGTGTTCTTTGTTTCTGTGTTCGAATTGTAACGTGAGAGATGAATTCGAACCTTCTTAGTCATAATGTTACTTCGTGACTTGTGTGCAGAAATTTGGTACTTCGCCAGCCAGACAAGTTTCTGTTGCAATGGGAGAGTTAAATCGTGCAGAGTATGTGTCGGTTGGCGAATCGCCTTCTTCTCCGGTCAATAATGCAAAGAAAGTTTCAGTGTTACCACTTGTGTTTCTCATCTTCTATGAGGTCTCAGGGGGTCCGTTTGGAGTTGAGGACAGTGTTGGGGCAGCTGGTCCTCTGTTAGCTCTTCTCGGGTTCTTGGTGTTTCCACTTATATGGAGTATTCCTGAAGCATTGGTTACTGCTGAGATGGGGACTATGTTCCCTGAAAATGGTGGTTATGTTGTTTGGGTTTCTTCTGCATTGGGTCCGTTTTGGGGGTTCCAGCAGGGTTGGATGAAATGGCTTAGTGGAGTTATTGATAATGCTCTATATCCTGTTTTGTTTCTCGATTACTTGAAGTCAGAGATCCCAGCTCTTGGTGGTGGTCTTCCAAAAGTCGCTGCGGCTTTGGCTTTGACAGTGGTTCTCACTTACATGAACTATAGAGGATTGACAATTGTTGGTTGGGTTGCTGTAATCCTTGGAGTTTTCTCAATCCTTCCTTTTGCAGTTATGGGACTTATATCTATTCCCAAGCTAAGACCAAGTAGATGGCTTGTGGTGAACCTAAAGGATGTTGACTGGAATCTGTACTGGAACACTCTTTTCTGGAATTTGAATTATTGGGATTCTATTAGCACATTGGCTGGAGAAGTGGAAAACCCAAACAAAACACTCCCCAAAGCACTGTTTTATGCTTTGATTTTGGTTGTTCTGGGGTACTTTTTTCCTCTTTTAAGTGGGACCGGAGCCATTCCGCTTAATCGTGACTTGTGGACAGATGGCTACTTCTCTGATGTTGCTAAAATTATTGGTGGGGCTTGGTTGAGTTGGTGGATCACAGGTGCTGCTGCCATATCGAATATGGGAATGTTCGTGGCTGAGATGAGCAGTGATTCTTTTCAACTTCTTGGTATGGCAGAACGCGGAATGCTGCCAGAGTTCTTTGGGAAACGGTCTCGTCATGGGACACCATTGATTGGGATACTATTCTCAGCCTCAGGTGTTGTTTTGCTTTCATGGTTGAGCTTCCAAGAAATCATAGCAGTAGAGAACTTCTTGTACTGCTTTGGAATGATTCTGGAATTCTTAGCCTTTATCATGCTAAGGATTAAACATCCAGCTGCATCTCGGCCTTTCAAGATTCCTGTGGGAACCGTTGGATCAATCCTGATGTGTATCCCTCCTACAATATTAATATGCGTAGTGTTGGCCCTTTCTGTGGTAAAAGTGATGATTGTGAGTCTAGCTGCTGTGGCAGTTGGCTTGTTGTTGCACCCCGGTCTCAGGTATGTTGAAAAGAAGAGATGGCTTAAATTCTCTGTGAGCGCTGACCTCCCTGATCTCCATTTTGCCAACTGAGACCGCCCCGACACCTTGGTATATTAACAGGAATTCACTTGTCACTCAGTTTCAAAGACAGGATAACTCGTTCTGAGATGATTGCAATGTGTTGGAGTTCTATATCTATACCAATTGATGCAATGGCAGACCAAAGATCTTGTAAGTTCATTCTGTTTTGATCTGTGCTCAAGTTGTAGTCTAATCATTGTTATCACCCTAGTTTTAGAATGGTGACGAACAGGAAAACAAAAAAGAAGCAAAATCTTAGTTGTTCAAGTAAGCGTCAATAGTAGCACTGCTTGTTCATGTTTTAATCCCACGATTCCACTGTCCTCAAAATATTCAATTAAGATGCCTTCAGCTCACACATCTCTTCCTCTCACGAGAAGAGTAGGACAATGCTATCTGTTTGAAATAAATTAGCAGAAGATTGAATATATGCATTCTTTAAACATTGCGATACGAATTCTCCTTTCTGATTGTCTTGTAAGTTCATGTGAAGAACAATATTGAAATTTGAAATGGTACAGCCGGGTGTAAAACGAAAGGTGGTTGAATTGTGAAAAGAAATCGGTGAATTTG

mRNA sequence

ATGGTAGAATTTCCTCCTCCTCCTTCTTCTTCTTCTAAATTTGGTACTTCGCCAGCCAGACAAGTTTCTGTTGCAATGGGAGAGTTAAATCGTGCAGAGTATGTGTCGGTTGGCGAATCGCCTTCTTCTCCGGTCAATAATGCAAAGAAAGTTTCAGTGTTACCACTTGTGTTTCTCATCTTCTATGAGGTCTCAGGGGGTCCGTTTGGAGTTGAGGACAGTGTTGGGGCAGCTGGTCCTCTGTTAGCTCTTCTCGGGTTCTTGGTGTTTCCACTTATATGGAGTATTCCTGAAGCATTGGTTACTGCTGAGATGGGGACTATGTTCCCTGAAAATGGTGGTTATGTTGTTTGGGTTTCTTCTGCATTGGGTCCGTTTTGGGGGTTCCAGCAGGGTTGGATGAAATGGCTTAGTGGAGTTATTGATAATGCTCTATATCCTGTTTTGTTTCTCGATTACTTGAAGTCAGAGATCCCAGCTCTTGGTGGTGGTCTTCCAAAAGTCGCTGCGGCTTTGGCTTTGACAGTGGTTCTCACTTACATGAACTATAGAGGATTGACAATTGTTGGTTGGGTTGCTGTAATCCTTGGAGTTTTCTCAATCCTTCCTTTTGCAGTTATGGGACTTATATCTATTCCCAAGCTAAGACCAAGTAGATGGCTTGTGGTGAACCTAAAGGATGTTGACTGGAATCTGTACTGGAACACTCTTTTCTGGAATTTGAATTATTGGGATTCTATTAGCACATTGGCTGGAGAAGTGGAAAACCCAAACAAAACACTCCCCAAAGCACTGTTTTATGCTTTGATTTTGGTTGTTCTGGGGTACTTTTTTCCTCTTTTAAGTGGGACCGGAGCCATTCCGCTTAATCGTGACTTGTGGACAGATGGCTACTTCTCTGATGTTGCTAAAATTATTGGTGGGGCTTGGTTGAGTTGGTGGATCACAGGTGCTGCTGCCATATCGAATATGGGAATGTTCGTGGCTGAGATGAGCAGTGATTCTTTTCAACTTCTTGGTATGGCAGAACGCGGAATGCTGCCAGAGTTCTTTGGGAAACGGTCTCGTCATGGGACACCATTGATTGGGATACTATTCTCAGCCTCAGGTGTTGTTTTGCTTTCATGGTTGAGCTTCCAAGAAATCATAGCAGTAGAGAACTTCTTGTACTGCTTTGGAATGATTCTGGAATTCTTAGCCTTTATCATGCTAAGGATTAAACATCCAGCTGCATCTCGGCCTTTCAAGATTCCTGTGGGAACCGTTGGATCAATCCTGATGTGTATCCCTCCTACAATATTAATATGCGTAGTGTTGGCCCTTTCTGTGGTAAAAGTGATGATTGTGAGTCTAGCTGCTGTGGCAGTTGGCTTGTTGTTGCACCCCGGTCTCAGGTATGTTGAAAAGAAGAGATGGCTTAAATTCTCTGTGAGCGCTGACCTCCCTGATCTCCATTTTGCCAACTGAGACCGCCCCGACACCTTGGTATATTAACAGGAATTCACTTGTCACTCAGTTTCAAAGACAGGATAACTCGTTCTGAGATGATTGCAATGTGTTGGAGTTCTATATCTATACCAATTGATGCAATGGCAGACCAAAGATCTTGTAAGTTCATTCTGTTTTGATCTGTGCTCAAGTTGTAGTCTAATCATTGTTATCACCCTAGTTTTAGAATGGTGACGAACAGGAAAACAAAAAAGAAGCAAAATCTTAGTTGTTCAAGTAAGCGTCAATAGTAGCACTGCTTGTTCATGTTTTAATCCCACGATTCCACTGTCCTCAAAATATTCAATTAAGATGCCTTCAGCTCACACATCTCTTCCTCTCACGAGAAGAGTAGGACAATGCTATCTGTTTGAAATAAATTAGCAGAAGATTGAATATATGCATTCTTTAAACATTGCGATACGAATTCTCCTTTCTGATTGTCTTGTAAGTTCATGTGAAGAACAATATTGAAATTTGAAATGGTACAGCCGGGTGTAAAACGAAAGGTGGTTGAATTGTGAAAAGAAATCGGTGAATTTG

Coding sequence (CDS)

ATGGTAGAATTTCCTCCTCCTCCTTCTTCTTCTTCTAAATTTGGTACTTCGCCAGCCAGACAAGTTTCTGTTGCAATGGGAGAGTTAAATCGTGCAGAGTATGTGTCGGTTGGCGAATCGCCTTCTTCTCCGGTCAATAATGCAAAGAAAGTTTCAGTGTTACCACTTGTGTTTCTCATCTTCTATGAGGTCTCAGGGGGTCCGTTTGGAGTTGAGGACAGTGTTGGGGCAGCTGGTCCTCTGTTAGCTCTTCTCGGGTTCTTGGTGTTTCCACTTATATGGAGTATTCCTGAAGCATTGGTTACTGCTGAGATGGGGACTATGTTCCCTGAAAATGGTGGTTATGTTGTTTGGGTTTCTTCTGCATTGGGTCCGTTTTGGGGGTTCCAGCAGGGTTGGATGAAATGGCTTAGTGGAGTTATTGATAATGCTCTATATCCTGTTTTGTTTCTCGATTACTTGAAGTCAGAGATCCCAGCTCTTGGTGGTGGTCTTCCAAAAGTCGCTGCGGCTTTGGCTTTGACAGTGGTTCTCACTTACATGAACTATAGAGGATTGACAATTGTTGGTTGGGTTGCTGTAATCCTTGGAGTTTTCTCAATCCTTCCTTTTGCAGTTATGGGACTTATATCTATTCCCAAGCTAAGACCAAGTAGATGGCTTGTGGTGAACCTAAAGGATGTTGACTGGAATCTGTACTGGAACACTCTTTTCTGGAATTTGAATTATTGGGATTCTATTAGCACATTGGCTGGAGAAGTGGAAAACCCAAACAAAACACTCCCCAAAGCACTGTTTTATGCTTTGATTTTGGTTGTTCTGGGGTACTTTTTTCCTCTTTTAAGTGGGACCGGAGCCATTCCGCTTAATCGTGACTTGTGGACAGATGGCTACTTCTCTGATGTTGCTAAAATTATTGGTGGGGCTTGGTTGAGTTGGTGGATCACAGGTGCTGCTGCCATATCGAATATGGGAATGTTCGTGGCTGAGATGAGCAGTGATTCTTTTCAACTTCTTGGTATGGCAGAACGCGGAATGCTGCCAGAGTTCTTTGGGAAACGGTCTCGTCATGGGACACCATTGATTGGGATACTATTCTCAGCCTCAGGTGTTGTTTTGCTTTCATGGTTGAGCTTCCAAGAAATCATAGCAGTAGAGAACTTCTTGTACTGCTTTGGAATGATTCTGGAATTCTTAGCCTTTATCATGCTAAGGATTAAACATCCAGCTGCATCTCGGCCTTTCAAGATTCCTGTGGGAACCGTTGGATCAATCCTGATGTGTATCCCTCCTACAATATTAATATGCGTAGTGTTGGCCCTTTCTGTGGTAAAAGTGATGATTGTGAGTCTAGCTGCTGTGGCAGTTGGCTTGTTGTTGCACCCCGGTCTCAGGTATGTTGAAAAGAAGAGATGGCTTAAATTCTCTGTGAGCGCTGACCTCCCTGATCTCCATTTTGCCAACTGA

Protein sequence

MVEFPPPPSSSSKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALVTAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPKVAAALALTVVLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLISIPKLRPSRWLVVNLKDVDWNLYWNTLFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWLSWWITGAAAISNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGVVLLSWLSFQEIIAVENFLYCFGMILEFLAFIMLRIKHPAASRPFKIPVGTVGSILMCIPPTILICVVLALSVVKVMIVSLAAVAVGLLLHPGLRYVEKKRWLKFSVSADLPDLHFAN
Homology
BLAST of CmaCh02G016540 vs. ExPASy Swiss-Prot
Match: Q9C6S5 (Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g31830 PE=2 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 2.1e-213
Identity = 362/473 (76.53%), Postives = 415/473 (87.74%), Query Frame = 0

Query: 17  SPARQVSVAMGELNRAEYVSVG--ESPSSP---VNNAKKVSVLPLVFLIFYEVSGGPFGV 76
           +P+  + ++  E N   Y SVG  E PSSP    +  +KVS+LPLVFLIFYEVSGGPFGV
Sbjct: 10  NPSASIEMSQYENNEVPYSSVGADEVPSSPPKATDKIRKVSMLPLVFLIFYEVSGGPFGV 69

Query: 77  EDSVGAAGPLLALLGFLVFPLIWSIPEALVTAEMGTMFPENGGYVVWVSSALGPFWGFQQ 136
           EDSV AAGPLLALLGF++FP IWSIPEAL+TAEMGTM+PENGGYVVWVSSALGPFWGFQQ
Sbjct: 70  EDSVNAAGPLLALLGFVIFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQ 129

Query: 137 GWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPKVAAALALTVVLTYMNYRGLTIVGW 196
           GWMKWLSGVIDNALYPVLFLDYLKS +PALG GLP+VA+ L LT++LTY+NYRGLTIVGW
Sbjct: 130 GWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGW 189

Query: 197 VAVILGVFSILPFAVMGLISIPKLRPSRWLVVNLKDVDWNLYWNTLFWNLNYWDSISTLA 256
           VAV++GVFSILPFAVMGLISIP+L PSRWLV++L +V+WNLY NTLFWNLNYWDSISTLA
Sbjct: 190 VAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNVNWNLYLNTLFWNLNYWDSISTLA 249

Query: 257 GEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWL 316
           GEVENPN TLPKALFY +ILV   Y FPLL+G GAIPL R+ WTDGYFSDVAK +GGAWL
Sbjct: 250 GEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWL 309

Query: 317 SWWITGAAAISNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGV 376
            WW+  AAA SNMGMF+AEMSSDSFQLLGMAERGMLPEFF KRSR+GTPL+GILFSASGV
Sbjct: 310 RWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLPEFFAKRSRYGTPLLGILFSASGV 369

Query: 377 VLLSWLSFQEIIAVENFLYCFGMILEFLAFIMLRIKHPAASRPFKIPVGTVGSILMCIPP 436
           VLLSWLSFQEI+A EN LYC GMILEF+AF+ +R+KHPAASRP+KIP+GT GSILMCIPP
Sbjct: 370 VLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPP 429

Query: 437 TILICVVLALSVVKVMIVSLAAVAVGLLLHPGLRYVEKKRWLKFSVSADLPDL 485
           TILIC V+ALS +KV  VS+  + +G L+HP L ++++KRW+KFS+S+DLPDL
Sbjct: 430 TILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFSISSDLPDL 482

BLAST of CmaCh02G016540 vs. ExPASy Swiss-Prot
Match: A2X8M8 (Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 1.0e-199
Identity = 339/484 (70.04%), Postives = 410/484 (84.71%), Query Frame = 0

Query: 3   EFPPPPSSSSKFGTSPARQVSVAMGELNRAEYVSV--GESPSSPVNNAKKVSVLPLVFLI 62
           E P   S+    G +P       MGE    EY  +  G++     ++A+ VS++PL+FLI
Sbjct: 41  EKPTVESAQPANGAAP-------MGECG-TEYRGLPDGDAGGPMPSSARTVSMIPLIFLI 100

Query: 63  FYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALVTAEMGTMFPENGGYVVWVS 122
           FYEVSGGPFG+EDSVGAAGPLLA++GFLV P+IWSIPEAL+TAE+G MFPENGGYVVWV+
Sbjct: 101 FYEVSGGPFGIEDSVGAAGPLLAIIGFLVLPVIWSIPEALITAELGAMFPENGGYVVWVA 160

Query: 123 SALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPKVAAALALTVVLTY 182
           SALGP+WGFQQGWMKWLSGVIDNALYPVLFLDYLKS +PALGGG P+  A + LT VLT 
Sbjct: 161 SALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGGGAPRAFAVVGLTAVLTL 220

Query: 183 MNYRGLTIVGWVAVILGVFSILPFAVMGLISIPKLRPSRWLVVNLKDVDWNLYWNTLFWN 242
           +NYRGLT+VGWVA+ LGVFS+LPF VMGLI++PKLRP+RWLV++L +VDWNLY NTLFWN
Sbjct: 221 LNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLRPARWLVIDLHNVDWNLYLNTLFWN 280

Query: 243 LNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFS 302
           LNYWDSISTLAGEV+NP KTLPKALFYA+I VV+ Y +PLL+GTGA+PL+R  WTDGYF+
Sbjct: 281 LNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAYLYPLLAGTGAVPLDRGQWTDGYFA 340

Query: 303 DVAKIIGGAWLSWWITGAAAISNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTP 362
           D+AK++GGAWL WW+  AAA+SNMGMFVAEMSSDS+QLLGMAERGMLP FF  RSR+GTP
Sbjct: 341 DIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSYQLLGMAERGMLPSFFAARSRYGTP 400

Query: 363 LIGILFSASGVVLLSWLSFQEIIAVENFLYCFGMILEFLAFIMLRIKHPAASRPFKIPVG 422
           L GILFSASGV+LLS +SFQEI+A ENFLYCFGM+LEF+AFI+ R++ P A+RP+++P+G
Sbjct: 401 LAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLLEFVAFILHRVRRPDAARPYRVPLG 460

Query: 423 TVGSILMCIPPTILICVVLALSVVKVMIVSLAAVAVGLLLHPGLRYVEKKRWLKFSVSAD 482
           T G + M +PPT LI VVLALS +KV +VSL AVA+GL+L P LR+VEKKRWL+FSV+ D
Sbjct: 461 TAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAMGLVLQPALRFVEKKRWLRFSVNPD 516

Query: 483 LPDL 485
           LP++
Sbjct: 521 LPEI 516

BLAST of CmaCh02G016540 vs. ExPASy Swiss-Prot
Match: Q6Z8D0 (Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 1.0e-199
Identity = 339/484 (70.04%), Postives = 410/484 (84.71%), Query Frame = 0

Query: 3   EFPPPPSSSSKFGTSPARQVSVAMGELNRAEYVSV--GESPSSPVNNAKKVSVLPLVFLI 62
           E P   S+    G +P       MGE    EY  +  G++     ++A+ VS++PL+FLI
Sbjct: 41  EKPTVESAQPANGAAP-------MGECG-TEYRGLPDGDAGGPMPSSARTVSMIPLIFLI 100

Query: 63  FYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALVTAEMGTMFPENGGYVVWVS 122
           FYEVSGGPFG+EDSVGAAGPLLA++GFLV P+IWSIPEAL+TAE+G MFPENGGYVVWV+
Sbjct: 101 FYEVSGGPFGIEDSVGAAGPLLAIIGFLVLPVIWSIPEALITAELGAMFPENGGYVVWVA 160

Query: 123 SALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPKVAAALALTVVLTY 182
           SALGP+WGFQQGWMKWLSGVIDNALYPVLFLDYLKS +PALGGG P+  A + LT VLT 
Sbjct: 161 SALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGGGAPRAFAVVGLTAVLTL 220

Query: 183 MNYRGLTIVGWVAVILGVFSILPFAVMGLISIPKLRPSRWLVVNLKDVDWNLYWNTLFWN 242
           +NYRGLT+VGWVA+ LGVFS+LPF VMGLI++PKLRP+RWLV++L +VDWNLY NTLFWN
Sbjct: 221 LNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLRPARWLVIDLHNVDWNLYLNTLFWN 280

Query: 243 LNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFS 302
           LNYWDSISTLAGEV+NP KTLPKALFYA+I VV+ Y +PLL+GTGA+PL+R  WTDGYF+
Sbjct: 281 LNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAYLYPLLAGTGAVPLDRGQWTDGYFA 340

Query: 303 DVAKIIGGAWLSWWITGAAAISNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTP 362
           D+AK++GGAWL WW+  AAA+SNMGMFVAEMSSDS+QLLGMAERGMLP FF  RSR+GTP
Sbjct: 341 DIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSYQLLGMAERGMLPSFFAARSRYGTP 400

Query: 363 LIGILFSASGVVLLSWLSFQEIIAVENFLYCFGMILEFLAFIMLRIKHPAASRPFKIPVG 422
           L GILFSASGV+LLS +SFQEI+A ENFLYCFGM+LEF+AFI+ R++ P A+RP+++P+G
Sbjct: 401 LAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLLEFVAFILHRVRRPDAARPYRVPLG 460

Query: 423 TVGSILMCIPPTILICVVLALSVVKVMIVSLAAVAVGLLLHPGLRYVEKKRWLKFSVSAD 482
           T G + M +PPT LI VVLALS +KV +VSL AVA+GL+L P LR+VEKKRWL+FSV+ D
Sbjct: 461 TAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAMGLVLQPALRFVEKKRWLRFSVNPD 516

Query: 483 LPDL 485
           LP++
Sbjct: 521 LPEI 516

BLAST of CmaCh02G016540 vs. ExPASy Swiss-Prot
Match: Q9FFL1 (Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 2.5e-198
Identity = 341/484 (70.45%), Postives = 403/484 (83.26%), Query Frame = 0

Query: 3   EFPPPPSSSSKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPLVFLIFY 62
           E PPPP         P   + V  G+           SP+  VN  KK++VLPLVFLIFY
Sbjct: 21  ENPPPP--------PPHISIGVTTGD--------PATSPARTVNQIKKITVLPLVFLIFY 80

Query: 63  EVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALVTAEMGTMFPENGGYVVWVSSA 122
           EVSGGPFG+EDSV AAGPLLA++GF+VFP IWSIPEAL+TAEMGTMFPENGGYVVWV+ A
Sbjct: 81  EVSGGPFGIEDSVKAAGPLLAIVGFIVFPFIWSIPEALITAEMGTMFPENGGYVVWVTLA 140

Query: 123 LGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPKVAAALALTVVLTYMN 182
           +GP+WGFQQGW+KWLSGVIDNALYP+LFLDYLKS IP LG G+P+VAA L LTV LTY+N
Sbjct: 141 MGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRVAAILVLTVALTYLN 200

Query: 183 YRGLTIVGWVAVILGVFSILPFAVMGLISIPKLRPSRWLVVN--LKDVDWNLYWNTLFWN 242
           YRGL+IVG  AV+LGVFSILPF VM  +SIPKL+PSRWLVV+  +K V+W+LY NTLFWN
Sbjct: 201 YRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMKGVNWSLYLNTLFWN 260

Query: 243 LNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFS 302
           LNYWDS+STL GEVENP+KTLP+ALFYAL+LVV  Y FP+L+GTGAI L++ LWTDGYF+
Sbjct: 261 LNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGAIALDQKLWTDGYFA 320

Query: 303 DVAKIIGGAWLSWWITGAAAISNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTP 362
           D+ K+IGG WL WWI  AAA SNMGMF+AEMSSDSFQLLGMAERGMLPE F KRSR+ TP
Sbjct: 321 DIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGMLPEVFAKRSRYRTP 380

Query: 363 LIGILFSASGVVLLSWLSFQEIIAVENFLYCFGMILEFLAFIMLRIKHPAASRPFKIPVG 422
            +GILFSASGV++LSWLSFQEI+A EN LYCFGM+LEF+ F+ LR+K+PAASRPFKIPVG
Sbjct: 381 WVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLRMKYPAASRPFKIPVG 440

Query: 423 TVGSILMCIPPTILICVVLALSVVKVMIVSLAAVAVGLLLHPGLRYVEKKRWLKFSVSAD 482
            +GS+LMCIPPT+LI V++A + +KV +VSLAA+ +GL+L P L+ VEKK WLKFS S+ 
Sbjct: 441 VLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLKQVEKKGWLKFSTSSH 488

Query: 483 LPDL 485
           LP+L
Sbjct: 501 LPNL 488

BLAST of CmaCh02G016540 vs. ExPASy Swiss-Prot
Match: Q9C6S4 (Probable polyamine transporter At1g31820 OS=Arabidopsis thaliana OX=3702 GN=At1g31820 PE=3 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 6.1e-189
Identity = 328/464 (70.69%), Postives = 382/464 (82.33%), Query Frame = 0

Query: 25  AMGELNRAEYVSVGESPSSPVNNAKKVSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLLAL 84
           A G L   +   VG S     N+ +KVS+LPLVFLIFYEVSGGPFG E SV AAGPLLAL
Sbjct: 10  AYGNLYDDDDGDVGGSSKEGNNSIQKVSMLPLVFLIFYEVSGGPFGAEGSVNAAGPLLAL 69

Query: 85  LGFLVFPLIWSIPEALVTAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNA 144
           LGF++FP IW IPEAL+TAEM TMFP NGG+VVWVSSALG FWGFQ GWMKWL GVIDNA
Sbjct: 70  LGFVIFPFIWCIPEALITAEMSTMFPINGGFVVWVSSALGTFWGFQVGWMKWLCGVIDNA 129

Query: 145 LYPVLFLDYLKSEIPALGGGLPKVAAALALTVVLTYMNYRGLTIVGWVAVILGVFSILPF 204
           LYPVLFLDYLKS +PAL  GLP+VA+ L LT++LTY+NYRGLTIVGW AV +GVFS+LPF
Sbjct: 130 LYPVLFLDYLKSAVPALATGLPRVASILILTLLLTYLNYRGLTIVGWTAVFMGVFSMLPF 189

Query: 205 AVMGLISIPKLRPSRWLVVNLKDVDWNLYWNTLFWNLNYWDSISTLAGEVENPNKTLPKA 264
           AVM L+SIP+L PSRWLV++L +V+WNLY NTL WNLNYWDS+STLAGEV NP KTLPKA
Sbjct: 190 AVMSLVSIPQLEPSRWLVMDLGNVNWNLYLNTLLWNLNYWDSVSTLAGEVANPKKTLPKA 249

Query: 265 LFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWLSWWITGAAAISNM 324
           L Y +I V L  F PLLSGTGAIPL+R+LWTDGY ++VAK IGG WL  W+  AAA SNM
Sbjct: 250 LCYGVIFVALSNFLPLLSGTGAIPLDRELWTDGYLAEVAKAIGGGWLQLWVQAAAATSNM 309

Query: 325 GMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGVVLLSWLSFQEIIA 384
           GMF+AEMSSDSFQLLGMAE G+LPE F +RSR+GTPL+GILFSASGV+LLS LSFQEIIA
Sbjct: 310 GMFLAEMSSDSFQLLGMAELGILPEIFAQRSRYGTPLLGILFSASGVLLLSGLSFQEIIA 369

Query: 385 VENFLYCFGMILEFLAFIMLRIKHPAASRPFKIPVGTVGSILMCIPPTILICVVLALSVV 444
            EN LYC GMILEF+AF+ LR KHPAASRP+KIPVGTVGSIL+C+PP +LIC+V+ LS +
Sbjct: 370 AENLLYCGGMILEFIAFVRLRKKHPAASRPYKIPVGTVGSILICVPPIVLICLVIVLSTI 429

Query: 445 KVMIVSLAAVAVGLLLHPGLRYVEKKRWLKFSVSADLPDLHFAN 489
           KV +VS   V +G L+ P L +++ K+W+KFSV +DL +    N
Sbjct: 430 KVALVSFVMVVIGFLMKPCLNHMDGKKWVKFSVCSDLAEFQKEN 473

BLAST of CmaCh02G016540 vs. TAIR 10
Match: AT1G31830.1 (Amino acid permease family protein )

HSP 1 Score: 743.0 bits (1917), Expect = 1.5e-214
Identity = 362/473 (76.53%), Postives = 415/473 (87.74%), Query Frame = 0

Query: 17  SPARQVSVAMGELNRAEYVSVG--ESPSSP---VNNAKKVSVLPLVFLIFYEVSGGPFGV 76
           +P+  + ++  E N   Y SVG  E PSSP    +  +KVS+LPLVFLIFYEVSGGPFGV
Sbjct: 10  NPSASIEMSQYENNEVPYSSVGADEVPSSPPKATDKIRKVSMLPLVFLIFYEVSGGPFGV 69

Query: 77  EDSVGAAGPLLALLGFLVFPLIWSIPEALVTAEMGTMFPENGGYVVWVSSALGPFWGFQQ 136
           EDSV AAGPLLALLGF++FP IWSIPEAL+TAEMGTM+PENGGYVVWVSSALGPFWGFQQ
Sbjct: 70  EDSVNAAGPLLALLGFVIFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQ 129

Query: 137 GWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPKVAAALALTVVLTYMNYRGLTIVGW 196
           GWMKWLSGVIDNALYPVLFLDYLKS +PALG GLP+VA+ L LT++LTY+NYRGLTIVGW
Sbjct: 130 GWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGW 189

Query: 197 VAVILGVFSILPFAVMGLISIPKLRPSRWLVVNLKDVDWNLYWNTLFWNLNYWDSISTLA 256
           VAV++GVFSILPFAVMGLISIP+L PSRWLV++L +V+WNLY NTLFWNLNYWDSISTLA
Sbjct: 190 VAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNVNWNLYLNTLFWNLNYWDSISTLA 249

Query: 257 GEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWL 316
           GEVENPN TLPKALFY +ILV   Y FPLL+G GAIPL R+ WTDGYFSDVAK +GGAWL
Sbjct: 250 GEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWL 309

Query: 317 SWWITGAAAISNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGV 376
            WW+  AAA SNMGMF+AEMSSDSFQLLGMAERGMLPEFF KRSR+GTPL+GILFSASGV
Sbjct: 310 RWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLPEFFAKRSRYGTPLLGILFSASGV 369

Query: 377 VLLSWLSFQEIIAVENFLYCFGMILEFLAFIMLRIKHPAASRPFKIPVGTVGSILMCIPP 436
           VLLSWLSFQEI+A EN LYC GMILEF+AF+ +R+KHPAASRP+KIP+GT GSILMCIPP
Sbjct: 370 VLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPP 429

Query: 437 TILICVVLALSVVKVMIVSLAAVAVGLLLHPGLRYVEKKRWLKFSVSADLPDL 485
           TILIC V+ALS +KV  VS+  + +G L+HP L ++++KRW+KFS+S+DLPDL
Sbjct: 430 TILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFSISSDLPDL 482

BLAST of CmaCh02G016540 vs. TAIR 10
Match: AT1G31830.2 (Amino acid permease family protein )

HSP 1 Score: 739.6 bits (1908), Expect = 1.6e-213
Identity = 361/462 (78.14%), Postives = 409/462 (88.53%), Query Frame = 0

Query: 28  ELNRAEYVSVG--ESPSSP---VNNAKKVSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLL 87
           E N   Y SVG  E PSSP    +  +KVS+LPLVFLIFYEVSGGPFGVEDSV AAGPLL
Sbjct: 5   ENNEVPYSSVGADEVPSSPPKATDKIRKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLL 64

Query: 88  ALLGFLVFPLIWSIPEALVTAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVID 147
           ALLGF++FP IWSIPEAL+TAEMGTM+PENGGYVVWVSSALGPFWGFQQGWMKWLSGVID
Sbjct: 65  ALLGFVIFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVID 124

Query: 148 NALYPVLFLDYLKSEIPALGGGLPKVAAALALTVVLTYMNYRGLTIVGWVAVILGVFSIL 207
           NALYPVLFLDYLKS +PALG GLP+VA+ L LT++LTY+NYRGLTIVGWVAV++GVFSIL
Sbjct: 125 NALYPVLFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFSIL 184

Query: 208 PFAVMGLISIPKLRPSRWLVVNLKDVDWNLYWNTLFWNLNYWDSISTLAGEVENPNKTLP 267
           PFAVMGLISIP+L PSRWLV++L +V+WNLY NTLFWNLNYWDSISTLAGEVENPN TLP
Sbjct: 185 PFAVMGLISIPQLEPSRWLVMDLGNVNWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLP 244

Query: 268 KALFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWLSWWITGAAAIS 327
           KALFY +ILV   Y FPLL+G GAIPL R+ WTDGYFSDVAK +GGAWL WW+  AAA S
Sbjct: 245 KALFYGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATS 304

Query: 328 NMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGVVLLSWLSFQEI 387
           NMGMF+AEMSSDSFQLLGMAERGMLPEFF KRSR+GTPL+GILFSASGVVLLSWLSFQEI
Sbjct: 305 NMGMFIAEMSSDSFQLLGMAERGMLPEFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEI 364

Query: 388 IAVENFLYCFGMILEFLAFIMLRIKHPAASRPFKIPVGTVGSILMCIPPTILICVVLALS 447
           +A EN LYC GMILEF+AF+ +R+KHPAASRP+KIP+GT GSILMCIPPTILIC V+ALS
Sbjct: 365 VAAENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALS 424

Query: 448 VVKVMIVSLAAVAVGLLLHPGLRYVEKKRWLKFSVSADLPDL 485
            +KV  VS+  + +G L+HP L ++++KRW+KFS+S+DLPDL
Sbjct: 425 SLKVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFSISSDLPDL 466

BLAST of CmaCh02G016540 vs. TAIR 10
Match: AT5G05630.1 (Amino acid permease family protein )

HSP 1 Score: 693.0 bits (1787), Expect = 1.8e-199
Identity = 341/484 (70.45%), Postives = 403/484 (83.26%), Query Frame = 0

Query: 3   EFPPPPSSSSKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPLVFLIFY 62
           E PPPP         P   + V  G+           SP+  VN  KK++VLPLVFLIFY
Sbjct: 21  ENPPPP--------PPHISIGVTTGD--------PATSPARTVNQIKKITVLPLVFLIFY 80

Query: 63  EVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALVTAEMGTMFPENGGYVVWVSSA 122
           EVSGGPFG+EDSV AAGPLLA++GF+VFP IWSIPEAL+TAEMGTMFPENGGYVVWV+ A
Sbjct: 81  EVSGGPFGIEDSVKAAGPLLAIVGFIVFPFIWSIPEALITAEMGTMFPENGGYVVWVTLA 140

Query: 123 LGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPKVAAALALTVVLTYMN 182
           +GP+WGFQQGW+KWLSGVIDNALYP+LFLDYLKS IP LG G+P+VAA L LTV LTY+N
Sbjct: 141 MGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRVAAILVLTVALTYLN 200

Query: 183 YRGLTIVGWVAVILGVFSILPFAVMGLISIPKLRPSRWLVVN--LKDVDWNLYWNTLFWN 242
           YRGL+IVG  AV+LGVFSILPF VM  +SIPKL+PSRWLVV+  +K V+W+LY NTLFWN
Sbjct: 201 YRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMKGVNWSLYLNTLFWN 260

Query: 243 LNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFS 302
           LNYWDS+STL GEVENP+KTLP+ALFYAL+LVV  Y FP+L+GTGAI L++ LWTDGYF+
Sbjct: 261 LNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGAIALDQKLWTDGYFA 320

Query: 303 DVAKIIGGAWLSWWITGAAAISNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTP 362
           D+ K+IGG WL WWI  AAA SNMGMF+AEMSSDSFQLLGMAERGMLPE F KRSR+ TP
Sbjct: 321 DIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGMLPEVFAKRSRYRTP 380

Query: 363 LIGILFSASGVVLLSWLSFQEIIAVENFLYCFGMILEFLAFIMLRIKHPAASRPFKIPVG 422
            +GILFSASGV++LSWLSFQEI+A EN LYCFGM+LEF+ F+ LR+K+PAASRPFKIPVG
Sbjct: 381 WVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLRMKYPAASRPFKIPVG 440

Query: 423 TVGSILMCIPPTILICVVLALSVVKVMIVSLAAVAVGLLLHPGLRYVEKKRWLKFSVSAD 482
            +GS+LMCIPPT+LI V++A + +KV +VSLAA+ +GL+L P L+ VEKK WLKFS S+ 
Sbjct: 441 VLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLKQVEKKGWLKFSTSSH 488

Query: 483 LPDL 485
           LP+L
Sbjct: 501 LPNL 488

BLAST of CmaCh02G016540 vs. TAIR 10
Match: AT1G31820.1 (Amino acid permease family protein )

HSP 1 Score: 661.8 bits (1706), Expect = 4.4e-190
Identity = 328/464 (70.69%), Postives = 382/464 (82.33%), Query Frame = 0

Query: 25  AMGELNRAEYVSVGESPSSPVNNAKKVSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLLAL 84
           A G L   +   VG S     N+ +KVS+LPLVFLIFYEVSGGPFG E SV AAGPLLAL
Sbjct: 10  AYGNLYDDDDGDVGGSSKEGNNSIQKVSMLPLVFLIFYEVSGGPFGAEGSVNAAGPLLAL 69

Query: 85  LGFLVFPLIWSIPEALVTAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNA 144
           LGF++FP IW IPEAL+TAEM TMFP NGG+VVWVSSALG FWGFQ GWMKWL GVIDNA
Sbjct: 70  LGFVIFPFIWCIPEALITAEMSTMFPINGGFVVWVSSALGTFWGFQVGWMKWLCGVIDNA 129

Query: 145 LYPVLFLDYLKSEIPALGGGLPKVAAALALTVVLTYMNYRGLTIVGWVAVILGVFSILPF 204
           LYPVLFLDYLKS +PAL  GLP+VA+ L LT++LTY+NYRGLTIVGW AV +GVFS+LPF
Sbjct: 130 LYPVLFLDYLKSAVPALATGLPRVASILILTLLLTYLNYRGLTIVGWTAVFMGVFSMLPF 189

Query: 205 AVMGLISIPKLRPSRWLVVNLKDVDWNLYWNTLFWNLNYWDSISTLAGEVENPNKTLPKA 264
           AVM L+SIP+L PSRWLV++L +V+WNLY NTL WNLNYWDS+STLAGEV NP KTLPKA
Sbjct: 190 AVMSLVSIPQLEPSRWLVMDLGNVNWNLYLNTLLWNLNYWDSVSTLAGEVANPKKTLPKA 249

Query: 265 LFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWLSWWITGAAAISNM 324
           L Y +I V L  F PLLSGTGAIPL+R+LWTDGY ++VAK IGG WL  W+  AAA SNM
Sbjct: 250 LCYGVIFVALSNFLPLLSGTGAIPLDRELWTDGYLAEVAKAIGGGWLQLWVQAAAATSNM 309

Query: 325 GMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGVVLLSWLSFQEIIA 384
           GMF+AEMSSDSFQLLGMAE G+LPE F +RSR+GTPL+GILFSASGV+LLS LSFQEIIA
Sbjct: 310 GMFLAEMSSDSFQLLGMAELGILPEIFAQRSRYGTPLLGILFSASGVLLLSGLSFQEIIA 369

Query: 385 VENFLYCFGMILEFLAFIMLRIKHPAASRPFKIPVGTVGSILMCIPPTILICVVLALSVV 444
            EN LYC GMILEF+AF+ LR KHPAASRP+KIPVGTVGSIL+C+PP +LIC+V+ LS +
Sbjct: 370 AENLLYCGGMILEFIAFVRLRKKHPAASRPYKIPVGTVGSILICVPPIVLICLVIVLSTI 429

Query: 445 KVMIVSLAAVAVGLLLHPGLRYVEKKRWLKFSVSADLPDLHFAN 489
           KV +VS   V +G L+ P L +++ K+W+KFSV +DL +    N
Sbjct: 430 KVALVSFVMVVIGFLMKPCLNHMDGKKWVKFSVCSDLAEFQKEN 473

BLAST of CmaCh02G016540 vs. TAIR 10
Match: AT3G19553.1 (Amino acid permease family protein )

HSP 1 Score: 536.2 bits (1380), Expect = 2.8e-152
Identity = 265/452 (58.63%), Postives = 339/452 (75.00%), Query Frame = 0

Query: 26  MGELNRAEYVSVGESPSSPVNNAKKVSVLPLVFLIFYEVSGGPFGVEDSV-GAAGPLLAL 85
           MGE    E   V +  SS    + K+++LPLVFLIFYEVSGGPFGVEDSV    GPLLAL
Sbjct: 1   MGE----EETIVNDENSSKPKPSPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLAL 60

Query: 86  LGFLVFPLIWSIPEALVTAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNA 145
           LGFL+FPLIWSIPEALVTAE+ T FPENGGYVVW+SSA GPFWGFQ+G+ KW SGV+DNA
Sbjct: 61  LGFLIFPLIWSIPEALVTAELATSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNA 120

Query: 146 LYPVLFLDYLKSEIPALGGGLPKVAAALALTVVLTYMNYRGLTIVGWVAVILGVFSILPF 205
           LYPVLFLDYLK   P L     +V A L +T  LTY+NYRGL IVG+ AV+L VFS+ PF
Sbjct: 121 LYPVLFLDYLKHSFPVLDHVAARVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPF 180

Query: 206 AVMGLISIPKLRPSRWLVVNLKDVDWNLYWNTLFWNLNYWDSISTLAGEVENPNKTLPKA 265
            VM L+++P +RP RWL V+ + ++W  Y+NT+FWNLNYWD  STLAGEV+ P KT PKA
Sbjct: 181 VVMALLAVPNIRPKRWLFVDTQKINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKA 240

Query: 266 LFYALILVVLGYFFPLLSGTGAIPLNRD-LWTDGYFSDVAKIIGGAWLSWWITGAAAISN 325
           LF A++LV+  Y  PL++GTGA+  +    W+DGYF++V  +IGG WL  WI  AAA+SN
Sbjct: 241 LFGAVLLVMGSYLIPLMAGTGALSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSN 300

Query: 326 MGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGVVLLSWLSFQEII 385
           +G+F AEMSSD+FQLLGM+E GMLP FF +RS++GTP I IL SA+GV+ LSW+SFQEII
Sbjct: 301 LGLFEAEMSSDAFQLLGMSEIGMLPAFFAQRSKYGTPTISILCSATGVIFLSWMSFQEII 360

Query: 386 AVENFLYCFGMILEFLAFIMLRIKHPAASRPFKIPVGTVGSILMCIPPTILICVVLALSV 445
              NFLY  GM+LEF AF+ LRIK P   RP+++P+ T G  ++C+PP++L+ +V+ L+ 
Sbjct: 361 EFLNFLYALGMLLEFAAFVKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAA 420

Query: 446 VKVMIVSLAAVAVGLLLHPGLRYVEKKRWLKF 476
            K  ++S   + +G  L+P L  V++K+W +F
Sbjct: 421 PKTFLISGVIIVLGFCLYPFLTLVKEKQWARF 448

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C6S52.1e-21376.53Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
A2X8M81.0e-19970.04Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 S... [more]
Q6Z8D01.0e-19970.04Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1... [more]
Q9FFL12.5e-19870.45Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1[more]
Q9C6S46.1e-18970.69Probable polyamine transporter At1g31820 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
Match NameE-valueIdentityDescription
AT1G31830.11.5e-21476.53Amino acid permease family protein [more]
AT1G31830.21.6e-21378.14Amino acid permease family protein [more]
AT5G05630.11.8e-19970.45Amino acid permease family protein [more]
AT1G31820.14.4e-19070.69Amino acid permease family protein [more]
AT3G19553.12.8e-15258.63Amino acid permease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePIRSFPIRSF006060AA_transportercoord: 32..488
e-value: 1.5E-76
score: 255.4
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 47..477
e-value: 2.4E-60
score: 206.4
NoneNo IPR availablePANTHERPTHR45826:SF10POLYAMINE TRANSPORTER PUT1coord: 16..486
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 56..450
e-value: 3.8E-37
score: 128.2
IPR044566Polyamine transporter RMV1-likePANTHERPTHR45826POLYAMINE TRANSPORTER PUT1coord: 16..486

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh02G016540.1CmaCh02G016540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902047 polyamine transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015203 polyamine transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity