CmaCh01G004730 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh01G004730
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	sphinganine C4-monooxygenase 1-like
Location	Cma_Chr01: 2390847 .. 2393901 (-)
RNA-Seq Expression	CmaCh01G004730
Synteny	CmaCh01G004730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCATCGTGGGATTCAGAGATTGAGCCAAGGATTATTGTAATTAGAAATTGAGAATTAGCGATCGATTTCCTGTGTTCTTCAAAAATTTCTCAACCAGAGTGGGGAAGATTATTCTGGATTTACTTCCAAACGCCCAGCGGATTTCCGATTTGGAGATTTATGCTTTGATTTTGTTCATCTTTTTGAATTCTTAACAAATCTATGGTTTTGTTTTTCTATCTCTGAATCTCTACCTTCTTCCTTCTCAATGGGATTCACCATCTCCGATGACTTGTTGGGTACTTTCGTCCCGATTTTCGTTTATTGGTTCTATTCTGGGATTTACGTGATTCTCGGTTTCTTCGAGAATTACCGACTGCACTCCAAGAAAGACGAGGATGAGAAGAACTTGGTTTCAAAATCCACTGTGGTTCGAGGCGTTCTCTTTCAACAATTCATTCAGGCTATTGTCGCCATCATCCTCTTTAAGGTCAGCTCCTACTTTCTATTTAATTTCTTTGCTAGTATTGCAATTAATAATCATCGTCGTCGTCGTCGCACTTGTCGTTATTACTGTAATTTAGTTTATTTCTGATCCTAATTATTGGTGGAACCTGCCCTAGAAAATTTCAACTGGGATTCATTTTGCTCATACACTTTGGTATAACTGTAGTACGATCTTCCTAAGATTTCCTTAGTTTAGCTACTGAGATTGAAAGTTTTGTTGTCTGTGGACCATTTGAGTTCTGACAAAATTAAAGTAACAACTCCTCTTCTCTTTATATGTGTTTTGATCGAAATTAGAGTCTGTCACACCACTGGACGCAGGGGTGAAGCCATCGCAGCAATTTACCATTTGCAAAATTTCGTTTTTTATATAATTAAACACTGCATATTTAAAAGAACATCTATTGCTAATGCTGGCAAAGTGAAAATCATTTTTTGTTACTGTAATTTGAATTGATGAAACAAAAATCTGAAAATCTTATAATTTAGTAGTCAACTCGAAAATTTCGATTCCATTCAGGAACAAAAGGGTTGATGATTCAAGCCGGAAATTGGAAGGATAAACTTGCAGTTTTTAGCATCCACTGTAAAGTTATATTAGTTGGTGTTAAGCTGTTAGCTTTTGACTTTTAAGTTTTTTGGATTTCAAACTCTAGGAATAGAGGTTCATAAGGAAAGAGTTTGGATGAATAATTTCAAAGCTGTGATCGATGGATTTGAATAAAAGTTTTATGCTATTCACCCCTTTTTTTATTCTTAAATTCCCTTTGAGGTTATCGTTTGGTTACAAATCGAATTGGTCAAAACAACACGGTATATCTCAATTGTATGAAAATTTAATCGTACTTTAGCTATAATTCGATTCTCTTGGATTTGTCTATGTAGTTTAGGTTACATATTTATTTGTGTAATCTTTAATGTTTTGGTTTACTTATATATATGTTTGAAAAAAAATATCCTCAAGAAGTATTGGGACAGTACCAATCCCCTTACGTCTTCTGCATTGTAGGAAGTCAACAACTCGTACGAGCTAAAACCTTGGTTCCGTACAATTGTGTCTTGTGTGTTGGATGTATTTACTACAATGTTATTGCTATTGCTTCTAAGCAATCTCTCTTTTTCTTTTTTCCTGTGAGTTTCATGCTAAATGAGATGTAGAACTGTAGTTCTTGTTGTGGATGTTCGTGTGAAATATAATTTCTGGAGTAAAACTATTATTAAAGTTAGCCTCATCTAAGTGCTGTTAAATGTGGCTTTGATCCAGTGGTTAAAGTGGCAAGTCATGAACTTAAATGTTTTCAGCTTATAGCATACTCCCAATTCTGGAAAGGAAAATGGAATCAATGTTAGATTCTGGCATTACTTACTGATATCATGTGGGTTACCAGCCCTGCACTTGCATCCTAGTTTGTATCCAAATTTACAATTGCATCTTGTTATGTTCAGGTGACTGGAAATGATGATGGGAGCATTGCAGTTCCAAAATCCTGGCCAGTGGTTGTTCTTCAATTTCTAACCGCGATGTTCGTTTTGGACACATGGCAATACTTCGCACACAGATACATGCATCATAACAAGTTTCTGTTCAGACACATTCATTCTCAGCACCATAAGCTTGTTGTTCCATATGCGTTTGGGGCTTTGTATAATCATCCTGTGGAAGGACTACTGCTCGACACAGTCGGTGGAGCGTTGTCGTTTCTAGTATCTGGTATGACTCCCAGAGTCTCTATCTTCTTCTTTTCCTTTGCAACTATTAAGACCGTAGACGATCACTGTGGATTATGGCTGCCTGGTAACCTCTTCCACGTCTTGTTTCGAAACAATACTGCATATCACGATGTCCACCATCAGCTATATGGTAGCAAGTTTAATTTCTCACAACCCTTCTTTGTTATATGGGATAGAATACTTGGAACCTACTTGCCTTACTCCTTGGAGAAGAGGGCAGGAGGCGGCTTTGAAGCACGGCCGAAGATTGAATAAATATTTTAAGCAGCCGTCTGTTGCTTATATTATATTATTTGTATCCCACAACAATATAATTTGTATATTACTCCTATTGTTGCGCTTTTTATACCCTTTTTTTTTCACATTGCCCTAACATAGGTTTGAAATATGAGTTGAGATGAGCTTAGGTTGTGTTTTGATATGTTTTCTTAAAACTTTTCTATGGCTTTAGATTAGTCACAATCATTGTGAGTGATGACTTTTGAAGAGCTGCTCCAATCCCAAGTGATAGCTTCTTCTTGTCTTTGTTAGAAAGGTTGTAGTTTCCAGTTATGCATCATAAGCTTGAACAGGGGCCATGTTTGGCTAGGTTGATGGGTTGATGAAGCTTTGGAACTAGATGGTTTGGTTTGGTTTGGTTTGGTTTGTTTGGACGCATGTTCGGAAATGATTTTGGAATGATTGAAATTACTATTCCGAAAAGGTGGAAAAGCTATTCTCTTATCTTATAGGTTGGATATTTGGAACATTTTAAATTTTATTTTGATAACGCTAGCGTTGTGTGTCGATCTTTGATCTCTATTACATGTTATATTTAATTGGAATCGTGATTACGTC

mRNA sequence

TTCATCGTGGGATTCAGAGATTGAGCCAAGGATTATTGTAATTAGAAATTGAGAATTAGCGATCGATTTCCTGTGTTCTTCAAAAATTTCTCAACCAGAGTGGGGAAGATTATTCTGGATTTACTTCCAAACGCCCAGCGGATTTCCGATTTGGAGATTTATGCTTTGATTTTGTTCATCTTTTTGAATTCTTAACAAATCTATGGTTTTGTTTTTCTATCTCTGAATCTCTACCTTCTTCCTTCTCAATGGGATTCACCATCTCCGATGACTTGTTGGGTACTTTCGTCCCGATTTTCGTTTATTGGTTCTATTCTGGGATTTACGTGATTCTCGGTTTCTTCGAGAATTACCGACTGCACTCCAAGAAAGACGAGGATGAGAAGAACTTGGTTTCAAAATCCACTGTGGTTCGAGGCGTTCTCTTTCAACAATTCATTCAGGCTATTGTCGCCATCATCCTCTTTAAGGTGACTGGAAATGATGATGGGAGCATTGCAGTTCCAAAATCCTGGCCAGTGGTTGTTCTTCAATTTCTAACCGCGATGTTCGTTTTGGACACATGGCAATACTTCGCACACAGATACATGCATCATAACAAGTTTCTGTTCAGACACATTCATTCTCAGCACCATAAGCTTGTTGTTCCATATGCGTTTGGGGCTTTGTATAATCATCCTGTGGAAGGACTACTGCTCGACACAGTCGGTGGAGCGTTGTCGTTTCTAGTATCTGGTATGACTCCCAGAGTCTCTATCTTCTTCTTTTCCTTTGCAACTATTAAGACCGTAGACGATCACTGTGGATTATGGCTGCCTGGTAACCTCTTCCACGTCTTGTTTCGAAACAATACTGCATATCACGATGTCCACCATCAGCTATATGGTAGCAAGTTTAATTTCTCACAACCCTTCTTTGTTATATGGGATAGAATACTTGGAACCTACTTGCCTTACTCCTTGGAGAAGAGGGCAGGAGGCGGCTTTGAAGCACGGCCGAAGATTGAATAAATATTTTAAGCAGCCGTCTGTTGCTTATATTATATTATTTGTATCCCACAACAATATAATTTGTATATTACTCCTATTGTTGCGCTTTTTATACCCTTTTTTTTTCACATTGCCCTAACATAGGTTTGAAATATGAGTTGAGATGAGCTTAGGTTGTGTTTTGATATGTTTTCTTAAAACTTTTCTATGGCTTTAGATTAGTCACAATCATTGTGAGTGATGACTTTTGAAGAGCTGCTCCAATCCCAAGTGATAGCTTCTTCTTGTCTTTGTTAGAAAGGTTGTAGTTTCCAGTTATGCATCATAAGCTTGAACAGGGGCCATGTTTGGCTAGGTTGATGGGTTGATGAAGCTTTGGAACTAGATGGTTTGGTTTGGTTTGGTTTGGTTTGTTTGGACGCATGTTCGGAAATGATTTTGGAATGATTGAAATTACTATTCCGAAAAGGTGGAAAAGCTATTCTCTTATCTTATAGGTTGGATATTTGGAACATTTTAAATTTTATTTTGATAACGCTAGCGTTGTGTGTCGATCTTTGATCTCTATTACATGTTATATTTAATTGGAATCGTGATTACGTC

Coding sequence (CDS)

ATGGGATTCACCATCTCCGATGACTTGTTGGGTACTTTCGTCCCGATTTTCGTTTATTGGTTCTATTCTGGGATTTACGTGATTCTCGGTTTCTTCGAGAATTACCGACTGCACTCCAAGAAAGACGAGGATGAGAAGAACTTGGTTTCAAAATCCACTGTGGTTCGAGGCGTTCTCTTTCAACAATTCATTCAGGCTATTGTCGCCATCATCCTCTTTAAGGTGACTGGAAATGATGATGGGAGCATTGCAGTTCCAAAATCCTGGCCAGTGGTTGTTCTTCAATTTCTAACCGCGATGTTCGTTTTGGACACATGGCAATACTTCGCACACAGATACATGCATCATAACAAGTTTCTGTTCAGACACATTCATTCTCAGCACCATAAGCTTGTTGTTCCATATGCGTTTGGGGCTTTGTATAATCATCCTGTGGAAGGACTACTGCTCGACACAGTCGGTGGAGCGTTGTCGTTTCTAGTATCTGGTATGACTCCCAGAGTCTCTATCTTCTTCTTTTCCTTTGCAACTATTAAGACCGTAGACGATCACTGTGGATTATGGCTGCCTGGTAACCTCTTCCACGTCTTGTTTCGAAACAATACTGCATATCACGATGTCCACCATCAGCTATATGGTAGCAAGTTTAATTTCTCACAACCCTTCTTTGTTATATGGGATAGAATACTTGGAACCTACTTGCCTTACTCCTTGGAGAAGAGGGCAGGAGGCGGCTTTGAAGCACGGCCGAAGATTGAATAA

Protein sequence

MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLFQQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFLFRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKTVDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEKRAGGGFEARPKIE

Homology

[Close]

BLAST of CmaCh01G004730 vs. ExPASy Swiss-Prot
Match: Q8VYI1 (Sphinganine C4-monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=SBH1 PE=1 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 4.9e-113
Identity = 192/254 (75.59%), Postives = 223/254 (87.80%), Query Frame = 0


Query: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60
           MGF +SD+LLGT  PI VYW YSGIYV L   E+YRLHSK +E+EKNLVSKS+VV+GVL 
Sbjct: 3   MGFAVSDELLGTVAPIVVYWLYSGIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLV 62

Query: 61  QQFIQAIVAIILFKVTGND-DGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKF 120
           QQ +QA+VAI+LF VTG+D +   A   S+ V+  QF+TAM VLDTWQYF HRYMH NKF
Sbjct: 63  QQVVQAVVAILLFTVTGSDAEADKAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKF 122

Query: 121 LFRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIK 180
           L++HIHSQHH+L+VPYA+GALYNHPVEGLLLDT+GGALSFLVSGM+PR SIFFFSFATIK
Sbjct: 123 LYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIK 182

Query: 181 TVDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLE 240
           TVDDHCGLWLPGNLFH++F+NN+AYHD+HHQLYG+K+NFSQPFFV+WDRILGTY+PYSLE
Sbjct: 183 TVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWDRILGTYMPYSLE 242

Query: 241 KRAGGGFEARPKIE 254
           KR  GGFEARP  E
Sbjct: 243 KREDGGFEARPTKE 256

[Close]

BLAST of CmaCh01G004730 vs. ExPASy Swiss-Prot
Match: Q9AST3 (Sphinganine C4-monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=SBH2 PE=1 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 3.9e-110
Identity = 185/252 (73.41%), Postives = 218/252 (86.51%), Query Frame = 0


Query: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60
           M F ISD+ LGTFVPI VYW YSG+Y+ LG  + YRLHSK DEDEKNLVSKS VV+GVL 
Sbjct: 2   MSFVISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLL 61

Query: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVL--QFLTAMFVLDTWQYFAHRYMHHNK 120
           QQ +QAI+++ILFK+TG+ D   A  + + +++L  QF+ AM V+DTWQYF HRYMH NK
Sbjct: 62  QQTLQAIISVILFKITGS-DADAATTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNK 121

Query: 121 FLFRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATI 180
           FL++HIHSQHH+L+VPY++GALYNHP+EGLLLDT+GGALSFL SGM+PR +IFFFSFATI
Sbjct: 122 FLYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFSFATI 181

Query: 181 KTVDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSL 240
           KTVDDHCGLWLPGN FH+ F NN+AYHDVHHQLYG+K+NFSQPFFV+WDRILGTYLPYSL
Sbjct: 182 KTVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWDRILGTYLPYSL 241

Query: 241 EKRAGGGFEARP 251
           EKRA GGFE RP
Sbjct: 242 EKRANGGFETRP 252

[Close]

BLAST of CmaCh01G004730 vs. ExPASy Swiss-Prot
Match: B8B4W4 (Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. indica OX=39946 GN=GL1-9 PE=3 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 5.4e-96
Identity = 166/251 (66.14%), Postives = 202/251 (80.48%), Query Frame = 0


Query: 5   ISDDLLGTFVPIFVYWFYSGIYVILGF---FENYRLHSKKDEDEKNLVSKSTVVRGVLFQ 64
           +SD+ +GTF PI +YW Y+G Y ++      E YRLH++ +E+EKNLV+   VVRGVL Q
Sbjct: 8   VSDETMGTFAPIALYWVYAGGYQLVLHRRPLERYRLHTRAEEEEKNLVALPAVVRGVLLQ 67

Query: 65  QFIQAIVAIILFKVTGNDDGSIAVPKSWPVVV--LQFLTAMFVLDTWQYFAHRYMHHNKF 124
           Q +QAIVA+ILF VT +    +  P   P+++   QFL AM V+D+WQYF HRYMH NKF
Sbjct: 68  QLVQAIVAMILFMVTSDSSAVVVQP---PIIIQAFQFLVAMLVMDSWQYFVHRYMHQNKF 127

Query: 125 LFRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIK 184
           L+RHIHSQHH+L+VPYA GALYNHP+EGLLLDTVGGA+SFLVSGMTPR S+FFF FA +K
Sbjct: 128 LYRHIHSQHHRLIVPYAIGALYNHPLEGLLLDTVGGAISFLVSGMTPRTSVFFFCFAVLK 187

Query: 185 TVDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLE 244
           TVDDHCGLWLP N+F  LF+NNTAYHDVHHQL GSK+N+SQPFF IWDRILGT++PY+L 
Sbjct: 188 TVDDHCGLWLPYNIFQSLFQNNTAYHDVHHQLQGSKYNYSQPFFSIWDRILGTHMPYNLV 247

Query: 245 KRAGGGFEARP 251
           +R  GGFEARP
Sbjct: 248 RRKEGGFEARP 255

[Close]

BLAST of CmaCh01G004730 vs. ExPASy Swiss-Prot
Match: Q0D4G3 (Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. japonica OX=39947 GN=GL1-9 PE=2 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 5.4e-96
Identity = 166/251 (66.14%), Postives = 202/251 (80.48%), Query Frame = 0


Query: 5   ISDDLLGTFVPIFVYWFYSGIYVILGF---FENYRLHSKKDEDEKNLVSKSTVVRGVLFQ 64
           +SD+ +GTF PI +YW Y+G Y ++      E YRLH++ +E+EKNLV+   VVRGVL Q
Sbjct: 8   VSDETMGTFAPIALYWVYAGGYQLVLHRRPLERYRLHTRAEEEEKNLVALPAVVRGVLLQ 67

Query: 65  QFIQAIVAIILFKVTGNDDGSIAVPKSWPVVV--LQFLTAMFVLDTWQYFAHRYMHHNKF 124
           Q +QAIVA+ILF VT +    +  P   P+++   QFL AM V+D+WQYF HRYMH NKF
Sbjct: 68  QLVQAIVAMILFMVTSDSSAVVVQP---PIIIQAFQFLVAMLVMDSWQYFVHRYMHQNKF 127

Query: 125 LFRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIK 184
           L+RHIHSQHH+L+VPYA GALYNHP+EGLLLDTVGGA+SFLVSGMTPR S+FFF FA +K
Sbjct: 128 LYRHIHSQHHRLIVPYAIGALYNHPLEGLLLDTVGGAISFLVSGMTPRTSVFFFCFAVLK 187

Query: 185 TVDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLE 244
           TVDDHCGLWLP N+F  LF+NNTAYHDVHHQL GSK+N+SQPFF IWDRILGT++PY+L 
Sbjct: 188 TVDDHCGLWLPYNIFQSLFQNNTAYHDVHHQLQGSKYNYSQPFFSIWDRILGTHMPYNLV 247

Query: 245 KRAGGGFEARP 251
           +R  GGFEARP
Sbjct: 248 RRKEGGFEARP 255

[Close]

BLAST of CmaCh01G004730 vs. ExPASy Swiss-Prot
Match: O94298 (Sphingolipid C4-hydroxylase sur2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sur2 PE=3 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 8.7e-54
Identity = 105/253 (41.50%), Postives = 148/253 (58.50%), Query Frame = 0


Query: 15  PIFVYWFYSGI-----YVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLFQQFIQAIVA 74
           P+ +YW  S       Y+ L  FE YR+H  ++   +N V +  VV+ VLFQQ  + +V 
Sbjct: 21  PVIIYWVASAFFGFLHYIELPVFEKYRIHPPEEIARRNRVPQMAVVKAVLFQQLCEVVVG 80

Query: 75  IILFKVTGNDDG------------------------SIAVPK---SWPVVVLQFLTAMFV 134
           I L    G  +                         +   PK   ++ V   Q+  A F+
Sbjct: 81  IALAMFEGYPEPIDEAKQMLRYEAFFSKNLPALLQVAPFAPKLAYNFIVPAFQYFFAFFI 140

Query: 135 LDTWQYFAHRYMHHNKFLFRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVS 194
           +D+WQYF HRY+H+NK L+  IH+ HH+L VPYA GALYNHP EGL+LDT G  +++L +
Sbjct: 141 IDSWQYFWHRYLHYNKKLYNMIHAHHHRLQVPYAMGALYNHPFEGLILDTFGAGVAYLAA 200

Query: 195 GMTPRVSIFFFSFATIKTVDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPF 236
           G++P+ ++ FF+ +T+KTVDDHCG   P +   + F NN  YHD+HHQ YG + NFSQPF
Sbjct: 201 GLSPQQAVIFFTLSTLKTVDDHCGYVFPYDPLQMFFANNARYHDLHHQPYGFQKNFSQPF 260

[Close]

BLAST of CmaCh01G004730 vs. ExPASy TrEMBL
Match: A0A6J1KH66 (sphinganine C4-monooxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493180 PE=3 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 6.5e-145
Identity = 253/253 (100.00%), Postives = 253/253 (100.00%), Query Frame = 0


Query: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60
           MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180
           FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT
Sbjct: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240
           VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CmaCh01G004730 vs. ExPASy TrEMBL
Match: A0A6J1GBQ9 (sphinganine C4-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452712 PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 1.5e-144
Identity = 251/253 (99.21%), Postives = 253/253 (100.00%), Query Frame = 0


Query: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60
           MGFTISDDLLGTFVPIFVYWFYSGIYV+LGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVLLGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGS+AVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSVAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180
           FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT
Sbjct: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240
           VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CmaCh01G004730 vs. ExPASy TrEMBL
Match: A0A5D3CZU2 (Sphinganine C4-monooxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1327G00180 PE=3 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 5.4e-139
Identity = 240/253 (94.86%), Postives = 248/253 (98.02%), Query Frame = 0


Query: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60
           M FTISDDLLGTFVPIFVYWFYSG+Y++LGFF+NYRLHSK DEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGS AVPKSWP+VVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSNAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180
           +RHIHSQHH+LVVPYAFGALYNHPVEGLLLDTVGGALSFL SGMTPRVSIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240
           VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFV WDRILGTY+PYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CmaCh01G004730 vs. ExPASy TrEMBL
Match: A0A1S3B426 (sphinganine C4-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103485785 PE=3 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 5.4e-139
Identity = 240/253 (94.86%), Postives = 248/253 (98.02%), Query Frame = 0


Query: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60
           M FTISDDLLGTFVPIFVYWFYSG+Y++LGFF+NYRLHSK DEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGS AVPKSWP+VVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSNAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180
           +RHIHSQHH+LVVPYAFGALYNHPVEGLLLDTVGGALSFL SGMTPRVSIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240
           VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFV WDRILGTY+PYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CmaCh01G004730 vs. ExPASy TrEMBL
Match: A0A6J1DY75 (sphinganine C4-monooxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111025684 PE=3 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 1.6e-138
Identity = 239/253 (94.47%), Postives = 249/253 (98.42%), Query Frame = 0


Query: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60
           M FTISD+LLGTFVPIFVYW YSGIYV+LGFFENYRLHSKKDEDEKNLVSK+TVVRGVLF
Sbjct: 1   MEFTISDELLGTFVPIFVYWLYSGIYVLLGFFENYRLHSKKDEDEKNLVSKATVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGS AVPKSWP+VVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSSAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180
           +R+IHSQHH+LVVPYAFGALYNHPVEGLLLDT+GGALSFLVSGMTPRVSIFFFSFATIKT
Sbjct: 121 YRYIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240
           VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSK+NFSQPFFV WDRILGTY+PYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKYNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CmaCh01G004730 vs. NCBI nr
Match: XP_022998593.1 (sphinganine C4-monooxygenase 1-like [Cucurbita maxima])

HSP 1 Score: 523.1 bits (1346), Expect = 1.3e-144
Identity = 253/253 (100.00%), Postives = 253/253 (100.00%), Query Frame = 0


Query: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60
           MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180
           FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT
Sbjct: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240
           VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CmaCh01G004730 vs. NCBI nr
Match: XP_023523598.1 (sphinganine C4-monooxygenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 522.3 bits (1344), Expect = 2.3e-144
Identity = 252/253 (99.60%), Postives = 253/253 (100.00%), Query Frame = 0


Query: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60
           MGFTISDDLLGTFVPIFVYWFYSGIYV+LGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVLLGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180
           FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT
Sbjct: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240
           VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CmaCh01G004730 vs. NCBI nr
Match: XP_022949326.1 (sphinganine C4-monooxygenase 1-like [Cucurbita moschata] >KAG6607119.1 Sphinganine C4-monooxygenase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036810.1 Sphinganine C4-monooxygenase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 521.9 bits (1343), Expect = 3.0e-144
Identity = 251/253 (99.21%), Postives = 253/253 (100.00%), Query Frame = 0


Query: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60
           MGFTISDDLLGTFVPIFVYWFYSGIYV+LGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVLLGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGS+AVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSVAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180
           FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT
Sbjct: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240
           VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CmaCh01G004730 vs. NCBI nr
Match: XP_008441706.1 (PREDICTED: sphinganine C4-monooxygenase 1-like [Cucumis melo] >KAA0032268.1 sphinganine C4-monooxygenase 1-like [Cucumis melo var. makuwa] >TYK17072.1 sphinganine C4-monooxygenase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 503.4 bits (1295), Expect = 1.1e-138
Identity = 240/253 (94.86%), Postives = 248/253 (98.02%), Query Frame = 0


Query: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60
           M FTISDDLLGTFVPIFVYWFYSG+Y++LGFF+NYRLHSK DEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGS AVPKSWP+VVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSNAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180
           +RHIHSQHH+LVVPYAFGALYNHPVEGLLLDTVGGALSFL SGMTPRVSIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240
           VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFV WDRILGTY+PYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CmaCh01G004730 vs. NCBI nr
Match: XP_022159270.1 (sphinganine C4-monooxygenase 1-like [Momordica charantia])

HSP 1 Score: 501.9 bits (1291), Expect = 3.2e-138
Identity = 239/253 (94.47%), Postives = 249/253 (98.42%), Query Frame = 0


Query: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60
           M FTISD+LLGTFVPIFVYW YSGIYV+LGFFENYRLHSKKDEDEKNLVSK+TVVRGVLF
Sbjct: 1   MEFTISDELLGTFVPIFVYWLYSGIYVLLGFFENYRLHSKKDEDEKNLVSKATVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGS AVPKSWP+VVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSSAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180
           +R+IHSQHH+LVVPYAFGALYNHPVEGLLLDT+GGALSFLVSGMTPRVSIFFFSFATIKT
Sbjct: 121 YRYIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240
           VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSK+NFSQPFFV WDRILGTY+PYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKYNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CmaCh01G004730 vs. TAIR 10
Match: AT1G69640.1 (sphingoid base hydroxylase 1 )

HSP 1 Score: 408.7 bits (1049), Expect = 3.5e-114
Identity = 192/254 (75.59%), Postives = 223/254 (87.80%), Query Frame = 0


Query: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60
           MGF +SD+LLGT  PI VYW YSGIYV L   E+YRLHSK +E+EKNLVSKS+VV+GVL 
Sbjct: 3   MGFAVSDELLGTVAPIVVYWLYSGIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLV 62

Query: 61  QQFIQAIVAIILFKVTGND-DGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKF 120
           QQ +QA+VAI+LF VTG+D +   A   S+ V+  QF+TAM VLDTWQYF HRYMH NKF
Sbjct: 63  QQVVQAVVAILLFTVTGSDAEADKAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKF 122

Query: 121 LFRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIK 180
           L++HIHSQHH+L+VPYA+GALYNHPVEGLLLDT+GGALSFLVSGM+PR SIFFFSFATIK
Sbjct: 123 LYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIK 182

Query: 181 TVDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLE 240
           TVDDHCGLWLPGNLFH++F+NN+AYHD+HHQLYG+K+NFSQPFFV+WDRILGTY+PYSLE
Sbjct: 183 TVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWDRILGTYMPYSLE 242

Query: 241 KRAGGGFEARPKIE 254
           KR  GGFEARP  E
Sbjct: 243 KREDGGFEARPTKE 256

[Close]

BLAST of CmaCh01G004730 vs. TAIR 10
Match: AT1G14290.1 (sphingoid base hydroxylase 2 )

HSP 1 Score: 399.1 bits (1024), Expect = 2.7e-111
Identity = 185/252 (73.41%), Postives = 218/252 (86.51%), Query Frame = 0


Query: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60
           M F ISD+ LGTFVPI VYW YSG+Y+ LG  + YRLHSK DEDEKNLVSKS VV+GVL 
Sbjct: 2   MSFVISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLL 61

Query: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVL--QFLTAMFVLDTWQYFAHRYMHHNK 120
           QQ +QAI+++ILFK+TG+ D   A  + + +++L  QF+ AM V+DTWQYF HRYMH NK
Sbjct: 62  QQTLQAIISVILFKITGS-DADAATTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNK 121

Query: 121 FLFRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATI 180
           FL++HIHSQHH+L+VPY++GALYNHP+EGLLLDT+GGALSFL SGM+PR +IFFFSFATI
Sbjct: 122 FLYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFSFATI 181

Query: 181 KTVDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSL 240
           KTVDDHCGLWLPGN FH+ F NN+AYHDVHHQLYG+K+NFSQPFFV+WDRILGTYLPYSL
Sbjct: 182 KTVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWDRILGTYLPYSL 241

Query: 241 EKRAGGGFEARP 251
           EKRA GGFE RP
Sbjct: 242 EKRANGGFETRP 252

[Close]

BLAST of CmaCh01G004730 vs. TAIR 10
Match: AT2G29390.3 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 77.0 bits (188), Expect = 2.4e-14
Identity = 69/236 (29.24%), Postives = 108/236 (45.76%), Query Frame = 0


Query: 10  LGTFVPIFVYWFYSGI-YVIL---GFFENYRLHSKKDEDEKNLVSKSTVVRGVLFQQFIQ 69
           +G+F+     +F SG+ Y+ L   GF  NY++ +K +  E        + R +L+   + 
Sbjct: 17  IGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARLLLYHCCVN 76

Query: 70  ---AIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFLFR 129
               + +  +F+  G  + S  +P SW VV  Q L    + D   Y+ HR + H K+L++
Sbjct: 77  LPLMMASYPVFRFMGM-ESSFPLP-SWKVVSAQILFYFIIEDFVFYWGHRIL-HTKWLYK 136

Query: 130 HIHSQHHKLVVPYAFGALYNHPVEGLLL---DTVGGALSFLVSGMTPRVSIFFFSFATIK 189
           ++HS HH+   P+   + Y HP E L L     VG AL    +G        +     I+
Sbjct: 137 NVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAL----TGPHLITLWLWMMLRVIE 196

Query: 190 TVDDHCGL---WLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGT 233
           TV+ HCG    W P N   +       +HD HH+L  +K       FV  D I GT
Sbjct: 197 TVEAHCGYHFPWSPSNFLPLY--GGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGT 240

[Close]

BLAST of CmaCh01G004730 vs. TAIR 10
Match: AT2G29390.2 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 77.0 bits (188), Expect = 2.4e-14
Identity = 69/236 (29.24%), Postives = 108/236 (45.76%), Query Frame = 0


Query: 10  LGTFVPIFVYWFYSGI-YVIL---GFFENYRLHSKKDEDEKNLVSKSTVVRGVLFQQFIQ 69
           +G+F+     +F SG+ Y+ L   GF  NY++ +K +  E        + R +L+   + 
Sbjct: 24  IGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARLLLYHCCVN 83

Query: 70  ---AIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFLFR 129
               + +  +F+  G  + S  +P SW VV  Q L    + D   Y+ HR + H K+L++
Sbjct: 84  LPLMMASYPVFRFMGM-ESSFPLP-SWKVVSAQILFYFIIEDFVFYWGHRIL-HTKWLYK 143

Query: 130 HIHSQHHKLVVPYAFGALYNHPVEGLLL---DTVGGALSFLVSGMTPRVSIFFFSFATIK 189
           ++HS HH+   P+   + Y HP E L L     VG AL    +G        +     I+
Sbjct: 144 NVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAL----TGPHLITLWLWMMLRVIE 203

Query: 190 TVDDHCGL---WLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGT 233
           TV+ HCG    W P N   +       +HD HH+L  +K       FV  D I GT
Sbjct: 204 TVEAHCGYHFPWSPSNFLPLY--GGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGT 247

[Close]

BLAST of CmaCh01G004730 vs. TAIR 10
Match: AT2G29390.1 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 75.5 bits (184), Expect = 6.9e-14
Identity = 71/246 (28.86%), Postives = 110/246 (44.72%), Query Frame = 0


Query: 10  LGTFVPIFVYWFYSGI-YVIL---GFFENYRLHSKKDEDEKNLVSKSTVVRGVLFQQFIQ 69
           +G+F+     +F SG+ Y+ L   GF  NY++ +K +  E        + R +L+   + 
Sbjct: 24  IGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARLLLYHCCVN 83

Query: 70  ---AIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFLFR 129
               + +  +F+  G  + S  +P SW VV  Q L    + D   Y+ HR + H K+L++
Sbjct: 84  LPLMMASYPVFRFMGM-ESSFPLP-SWKVVSAQILFYFIIEDFVFYWGHRIL-HTKWLYK 143

Query: 130 HIHSQHHKLVVPYAFGALYNHPVEGLLL---DTVGGALSFLVSGMTPRVSIFFFSFATIK 189
           ++HS HH+   P+   + Y HP E L L     VG AL    +G        +     I+
Sbjct: 144 NVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAL----TGPHLITLWLWMMLRVIE 203

Query: 190 TVDDHCGL---WLPGN----------LFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIW 233
           TV+ HCG    W P N          L    F  +  +HD HH+L  +K       FV  
Sbjct: 204 TVEAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLLYTKSGNYSSTFVYM 259

The following BLAST results are available for this feature:

BLAST of CmaCh01G004730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYI1	4.9e-113	75.59	Sphinganine C4-monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=SBH1 PE=1 SV=1	[more]
Q9AST3	3.9e-110	73.41	Sphinganine C4-monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=SBH2 PE=1 SV=1	[more]
B8B4W4	5.4e-96	66.14	Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. indica OX=39...	[more]
Q0D4G3	5.4e-96	66.14	Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. japonica OX=...	[more]
O94298	8.7e-54	41.50	Sphingolipid C4-hydroxylase sur2 OS=Schizosaccharomyces pombe (strain 972 / ATCC...	[more]

BLAST of CmaCh01G004730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KH66	6.5e-145	100.00	sphinganine C4-monooxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493180 ...	[more]
A0A6J1GBQ9	1.5e-144	99.21	sphinganine C4-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11145271...	[more]
A0A5D3CZU2	5.4e-139	94.86	Sphinganine C4-monooxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A1S3B426	5.4e-139	94.86	sphinganine C4-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103485785 PE=3...	[more]
A0A6J1DY75	1.6e-138	94.47	sphinganine C4-monooxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC1110256...	[more]

BLAST of CmaCh01G004730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022998593.1	1.3e-144	100.00	sphinganine C4-monooxygenase 1-like [Cucurbita maxima]	[more]
XP_023523598.1	2.3e-144	99.60	sphinganine C4-monooxygenase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022949326.1	3.0e-144	99.21	sphinganine C4-monooxygenase 1-like [Cucurbita moschata] >KAG6607119.1 Sphingani...	[more]
XP_008441706.1	1.1e-138	94.86	PREDICTED: sphinganine C4-monooxygenase 1-like [Cucumis melo] >KAA0032268.1 sphi...	[more]
XP_022159270.1	3.2e-138	94.47	sphinganine C4-monooxygenase 1-like [Momordica charantia]	[more]

BLAST of CmaCh01G004730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G69640.1	3.5e-114	75.59	sphingoid base hydroxylase 1	[more]
AT1G14290.1	2.7e-111	73.41	sphingoid base hydroxylase 2	[more]
AT2G29390.3	2.4e-14	29.24	sterol 4-alpha-methyl-oxidase 2-2	[more]
AT2G29390.2	2.4e-14	29.24	sterol 4-alpha-methyl-oxidase 2-2	[more]
AT2G29390.1	6.9e-14	28.86	sterol 4-alpha-methyl-oxidase 2-2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 97..232 e-value: 2.2E-23 score: 83.1
None	No IPR available	PANTHER	PTHR11863:SF190	BNACNNG58690D PROTEIN	coord: 1..251
None	No IPR available	PANTHER	PTHR11863	STEROL DESATURASE	coord: 1..251

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh01G004730.1	CmaCh01G004730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016491	oxidoreductase activity