CmaCh00G000740 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh00G000740
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	cytochrome P450 CYP72A219-like
Location	Cma_Chr00: 4068400 .. 4071819 (-)
RNA-Seq Expression	CmaCh00G000740
Synteny	CmaCh00G000740

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAAATCTCTCTATTTAGATGGAGATATTGGCGAGTATTAGGGTTTCCATTTTGGCGGTGGTTGTGGTGTGGTTAGCCGTATGGGGATGGAGATTTCTGAACTGGATTTGGCTGAGGCCGAAGAAGCTTGAGAGGTGCCTCAGAAAGCAAGGCTTCGCCGGGAATCCTTACCGGCTTTTCTCCGGCGACATGAAGGACATAGCCGCCATGAGGGAGCAGGCCATATCCAGCCCCATGAACTTCTCCCATGATATTTCTCCACGTGTCATCCCCTCCATAAATCATACCATCCACAAATATGGTAACTTTAATTTTTGTGCTATAGATATCATATTTGATGTTTGTCGTTTTATTAAAATCAAATATCTTCTTTCTTTATTTTTTTTATTTTTTATTTGTAATTTATTTAATAATTATAAATATAAAACTTTCCCTCCACAATAAATATTCTCTAGTTTATATTAAATACAGGAAAAAATTCATTCATTTGGACTGGACCATATCCAAGAGTGCACATCGTGGACCCCGATCAACTCAAAGCTGCACTTTGTTTAATGCATGATATTCAAAAGCCGACTATCAACCCTCTTGCAAGGTTGCTTTTCGATGGACTTACTAATCATGAAGGACCCAAATGGGTTAAGCATAGAAAGATAATCAATCCAGCCTTTCATTTCAAACACTTGAAGGTATGCTTTCTTCTTGTAATTGGATATCAAAACTTAACTTGAATAAATAAACTCATAATTAGTATTTATAAGTTTAATTTTGTATACTTGTTGCTTTCAATACGATTGGTAACAATTTGTAACAGCTCAAGCTCACCGCTAGTAGATATTGTCCGTTTTGGCCCGTTACATATCGTTGTTAGCCTTACGGTTTTAAAACGTGTTGGATAGGGAGAGGTTTCCAAACTCTTATAAAGAATACTTCATTTCCTTCTCCAACCAATGTGGGATCTCACAATCCACCTCTTATGTGCCCCAGCATTCTCGCTGACACTTGTTCCTCTCGACAATCGATGTGGGATCTCACAATTCACCTCTTTTCGAGGCTCAGCGTTCTTGCTGGCACACTGCCCGGTGTCTGGCTCTAATACTGTTTGTAATAGCCCAAATCTACCACTAGCATCAAATATTGTTTTTTTTTTTTGGCTTTCCCTTTCCCTCAATGTTCTTAAAATATGTCTGCTAAGGAGAGGTTTTTGTACCCTTGTAAACATGTTTGATTGTGAGATTCCATGTAGGTTGGGAGGAAAATGAAACATTCTTTATAAGGGTATAAAAACCTCTCCTTAGCATATACGTTTTAAAATCTTTGAGGGGAAACCCGAAAGGGAAAGCTTAAAGAGGACAATATCTATTAGCGGTAGGGTTAGGCTGTTACACGACTATCTATCCATCTAACCATGTTAGATTCTTTAAACATACATTTAGAAGTTGCTTCTATATTCTTTGATCAACAATATTTTTGCGAGTCTAATTGTTCATATATATGCTTAAATTGCTATATACAGGACATGGTGCCTGCAATATGTGAGAGTTGCACTGAGATGATTAGGAAATGGGAAGAAGTGGTCTCAAAAGATGGATATTATGAATTGGATGTGATGCCTCATTTGGAAAACTTGGCAGCTGATGTGATTTCTCGAACAGCATTTGGAAGCAGCTACGAAGAAGGAAAAATGATCTTTATACGTCTAAAACAACTTACGAATCTAGTTGTTCAATCTATATCTACAGTTTACATACCCGGATGGAGGTGAACTCAGGCACAATTCAATTCAATTTAGAAATGAATATGAATATGAGTCCTTTTTAGACTTTTCCTTTTGAACTTCTCCTCGAGATTTTTAAAACGTGTCTCGGAAAAGGTTTTCACACTCATAAAGAATGTTTTGTTTCCCTCTCTAACTGATATGGGATCTCACAATTCACCCTCCTTCGAGACCCAGCGTCGTCGTTGGCACTCGTTCCTCTCACAATCCACCCTTTTCGAGACCCAGCGTCCTCGCTAACACTCGTTCCTCTCTCCAACCAATGTAGAATCTAACAATCCACCCCCTTTGGCCCGAGTGTCCTCACTGACACACGTTCCTCTTTCCATTCGATGTGGGATCTCACAATTCACCCCCTTCAAGGCCTAGCCTCCTCGTTGGCACACCGCCTGGTGTCTGATTCTGATACCATTTATAACAGCTCAAGCCAACCACTAGCAAATATTGTCCTCTTTGGCCTCTCCCTTTTAGGCTTTCCGTTTTGGGCTTCCTCTAAGGTTTTTAAAACGAGTCTGCTAGAAAGAGGTTTACACACACTTATATGAAATGTTTCGTTTCCCACTCCAACCAATGTGAAATCTCACAATCTACGCCCTTTAAGGCCAGCGTTCTCGCTGACACTCGTTTCTCTCTCCAATCGATCTCACACAAGCAATATTTATAAAATTTCTTTTAACATCTCATTATAACGTTTGTGCAGGTTTGTACCAACAAAGTCAAACACTAGAATGAAAAAGATAAGCAAAGAATTAAAAACGTTGCTCTTGGATGTTATCAACAAAAGGCAAAGGGCTATGATGGCAAGTGAAGCTATGCAAGTATCTATTAAGCATCCTCTTAGAATCAAATTTGAAAGAAATTCGAGAGCACGGAAACAACGATGTCGGAATGAGTATCGAAGACATCGTAGAAGAATGTAGAATCTTTTATCTTGCTGGCCAAGAAACCACAGCAATGCTACTAGTTTGGACTATGGTTTTATTGAGTTCATACACAAAATGGCAAGATCGAGCAAGAGAAGAGGTCCTACAAGTCTTTGGAAACAACAAACCAGATTTTGATGGTTTAAATCGCCTAAAAATTGTGAGTAACACTCTCTTCTATGTATTTTCATTTTCATTTTATTCGTTAAATTTTTATCTTATTCTCAACATTTAGGTTACGATGATATTGAACGAAGTCCTGAGATTATACCCACCAGCACCGTTGCTTACTCGTCTCGTTCAAAACGAAACAAAGCTTGAAAAATTGAGTTTACCAACGGGAGTGATGCTGAGTTTTCCAATTGTTCTTATCCATCGTGATAAGAAGATATGGGGTGATGATGCAAATGAGTTTAATCCCGATAGATTTGCTGAGGGAGTTTCAAAAGCGACACAAAATCAAGCTGGGTTCTTCCCATTTGGATGGGGTCCTCGCATATGCATCGGACAGAACTTTGCTATAATTGAGGCCAAAATTGCTCTATCCATGATTCTACAACATTTTTATTTTGAGCTTTCTTCGTCTTATACGCATGCTCCGACCTCCAATTTAACTATTAGACCTCAACATGGAACTGATATCATGCTACGTAAACTTTAGCCAGGTAAACAAAAAAAAAAATCAATGTACATATAGTTTTTCGAATGTCAAGAATAAAG

mRNA sequence

AGAAATCTCTCTATTTAGATGGAGATATTGGCGAGTATTAGGGTTTCCATTTTGGCGGTGGTTGTGGTGTGGTTAGCCGTATGGGGATGGAGATTTCTGAACTGGATTTGGCTGAGGCCGAAGAAGCTTGAGAGGTGCCTCAGAAAGCAAGGCTTCGCCGGGAATCCTTACCGGCTTTTCTCCGGCGACATGAAGGACATAGCCGCCATGAGGGAGCAGGCCATATCCAGCCCCATGAACTTCTCCCATGATATTTCTCCACGTGTCATCCCCTCCATAAATCATACCATCCACAAATATGGAAAAAATTCATTCATTTGGACTGGACCATATCCAAGAGTGCACATCGTGGACCCCGATCAACTCAAAGCTGCACTTTGTTTAATGCATGATATTCAAAAGCCGACTATCAACCCTCTTGCAAGGTTGCTTTTCGATGGACTTACTAATCATGAAGGACCCAAATGGGTTAAGCATAGAAAGATAATCAATCCAGCCTTTCATTTCAAACACTTGAAGGACATGGTGCCTGCAATATGTGAGAGTTGCACTGAGATGATTAGGAAATGGGAAGAAGTGGTCTCAAAAGATGGATATTATGAATTGGATGTGATGCCTCATTTGGAAAACTTGGCAGCTGATGTGATTTCTCGAACAGCATTTGGAAGCAGCTACGAAGAAGGAAAAATGATCTTTATACGTCTAAAACAACTTACGAATCTAGTTGTTCAATCTATATCTACAGTTTACATACCCGGATGGAGGTTTGTACCAACAAAGTCAAACACTAGAATGAAAAAGATAAGCAAAGAATTAAAAACGTTGCTCTTGGATGTTATCAACAAAAGGCAAAGGGCTATGATGGCAAGTGAAGCTATGCAAGTATCTATTAAGCATCCTCTTAGAATCAAATTTGAAAGAAATTCGAGAGCACGGAAACAACGATGTCGGAATGAGTATCGAAGACATCGTAGAAGAATGTAGAATCTTTTATCTTGCTGGCCAAGAAACCACAGCAATGCTACTAGTTTGGACTATGGTTTTATTGAGTTCATACACAAAATGGCAAGATCGAGCAAGAGAAGAGGTCCTACAAGTCTTTGGAAACAACAAACCAGATTTTGATGGTTTAAATCGCCTAAAAATTGTTACGATGATATTGAACGAAGTCCTGAGATTATACCCACCAGCACCGTTGCTTACTCGTCTCGTTCAAAACGAAACAAAGCTTGAAAAATTGAGTTTACCAACGGGAGTGATGCTGAGTTTTCCAATTGTTCTTATCCATCGTGATAAGAAGATATGGGGTGATGATGCAAATGAGTTTAATCCCGATAGATTTGCTGAGGGAGTTTCAAAAGCGACACAAAATCAAGCTGGGTTCTTCCCATTTGGATGGGGTCCTCGCATATGCATCGGACAGAACTTTGCTATAATTGAGGCCAAAATTGCTCTATCCATGATTCTACAACATTTTTATTTTGAGCTTTCTTCGTCTTATACGCATGCTCCGACCTCCAATTTAACTATTAGACCTCAACATGGAACTGATATCATGCTACGTAAACTTTAGCCAGGTAAACAAAAAAAAAAATCAATGTACATATAGTTTTTCGAATGTCAAGAATAAAG

Coding sequence (CDS)

ATGGAGATATTGGCGAGTATTAGGGTTTCCATTTTGGCGGTGGTTGTGGTGTGGTTAGCCGTATGGGGATGGAGATTTCTGAACTGGATTTGGCTGAGGCCGAAGAAGCTTGAGAGGTGCCTCAGAAAGCAAGGCTTCGCCGGGAATCCTTACCGGCTTTTCTCCGGCGACATGAAGGACATAGCCGCCATGAGGGAGCAGGCCATATCCAGCCCCATGAACTTCTCCCATGATATTTCTCCACGTGTCATCCCCTCCATAAATCATACCATCCACAAATATGGAAAAAATTCATTCATTTGGACTGGACCATATCCAAGAGTGCACATCGTGGACCCCGATCAACTCAAAGCTGCACTTTGTTTAATGCATGATATTCAAAAGCCGACTATCAACCCTCTTGCAAGGTTGCTTTTCGATGGACTTACTAATCATGAAGGACCCAAATGGGTTAAGCATAGAAAGATAATCAATCCAGCCTTTCATTTCAAACACTTGAAGGACATGGTGCCTGCAATATGTGAGAGTTGCACTGAGATGATTAGGAAATGGGAAGAAGTGGTCTCAAAAGATGGATATTATGAATTGGATGTGATGCCTCATTTGGAAAACTTGGCAGCTGATGTGATTTCTCGAACAGCATTTGGAAGCAGCTACGAAGAAGGAAAAATGATCTTTATACGTCTAAAACAACTTACGAATCTAGTTGTTCAATCTATATCTACAGTTTACATACCCGGATGGAGGTTTGTACCAACAAAGTCAAACACTAGAATGAAAAAGATAAGCAAAGAATTAAAAACGTTGCTCTTGGATGTTATCAACAAAAGGCAAAGGGCTATGATGGCAAGTGAAGCTATGCAAGTATCTATTAAGCATCCTCTTAGAATCAAATTTGAAAGAAATTCGAGAGCACGGAAACAACGATGTCGGAATGAGTATCGAAGACATCGTAGAAGAATGTAG

Protein sequence

MEILASIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMKDIAAMREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAALCLMHDIQKPTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTEMIRKWEEVVSKDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQSISTVYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASEAMQVSIKHPLRIKFERNSRARKQRCRNEYRRHRRRM

Homology

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BLAST of CmaCh00G000740 vs. ExPASy Swiss-Prot
Match: H2DH21 (Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 2.9e-86
Identity = 161/308 (52.27%), Postives = 212/308 (68.83%), Query Frame = 0


Query: 1   MEILASIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMKD 60
           ME++  +  S  A+VVV L   GWR  NW+WLRP+KLE+ LR QGF GN YRLF GD+K+
Sbjct: 1   MELVLKLISSFCAIVVVILL--GWRIFNWVWLRPRKLEKYLRNQGFNGNSYRLFFGDVKE 60

Query: 61  IAAMREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAAL 120
           +  M ++A S P+N   DI PR+IP     I  YGKNSF+W GP P VHI++PD +K  L
Sbjct: 61  MIVMLKEAKSKPINLYDDIIPRIIPLNQKIITNYGKNSFLWLGPKPMVHIMNPDHIKDVL 120

Query: 121 CLMHDIQKPTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTEM 180
              +  QKP  NPL +LL  G+ + EG +W KHRK+INPAFH + LK+M+PAI  S +E+
Sbjct: 121 SKFYQFQKPRHNPLTKLLATGVADAEGDRWAKHRKLINPAFHLEKLKNMLPAIYLSSSEI 180

Query: 181 IRKWEEVVSKDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQSI 240
           + KWEE+VS  G +ELDV+P+LE L +DVISRTAFGSSYEEG+ IF   ++   L++Q+ 
Sbjct: 181 VTKWEEMVSTKGQFELDVLPYLETLTSDVISRTAFGSSYEEGRKIFQLQREQAELIIQAS 240

Query: 241 STVYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASEAMQVSIKHPLRIKFE 300
            T+Y+PG RF+PTK N RMK+I+KE+K  L  +INKR +AM A E  + S    L I  E
Sbjct: 241 QTIYLPGMRFLPTKRNKRMKEIAKEVKIALKSIINKRLKAMEAGE--RSSHDDLLGILLE 300

Query: 301 RNSRARKQ 309
            NS+  KQ
Sbjct: 301 SNSKEIKQ 304

[Close]

BLAST of CmaCh00G000740 vs. ExPASy Swiss-Prot
Match: A0A0S2IHL2 (Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.3e-81
Identity = 138/283 (48.76%), Postives = 195/283 (68.90%), Query Frame = 0


Query: 3   ILASIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMKDIA 62
           ++   +++    V V +  W W+ LNW+W+ P+KLE  LRKQGF GN YRLF GD+K+ +
Sbjct: 5   VVTYTKIAAAVAVAVVVVGWAWKVLNWVWVSPRKLEESLRKQGFRGNSYRLFYGDLKESS 64

Query: 63  AMREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAALCL 122
            M  +A   P+N S D   RV P I+ T+ KYGK+SFIW GP PRV I+DP+ +K  +  
Sbjct: 65  EMTRKAKLKPINLSDDPVLRVRPFIHQTVKKYGKSSFIWIGPTPRVQIMDPEIIKEIMVK 124

Query: 123 MHDIQKPTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTEMIR 182
            +   KP  NPL +L  DGL NHEG  W KHRK++NPAFH + LK M+PA+  SC EM+ 
Sbjct: 125 SYKFNKPKRNPLVKLFADGLANHEGELWAKHRKLLNPAFHLERLKCMLPAMYFSCIEMVS 184

Query: 183 KWEEVVSKDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQSIST 242
           KW++++SKDG  ELDV P L+ L +DVIS TAFGSSYEEG ++F    +   LV++++ +
Sbjct: 185 KWDKMISKDGSRELDVWPFLQRLTSDVISHTAFGSSYEEGNIVFELQTEQAELVMKTLQS 244

Query: 243 VYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASE 286
           VYIPGW ++PTK N +MK+I ++ ++ L+++INK+ +AM A E
Sbjct: 245 VYIPGWSYLPTKRNRKMKEIDRKTQSCLMNIINKKTKAMQAGE 287

[Close]

BLAST of CmaCh00G000740 vs. ExPASy Swiss-Prot
Match: Q05047 (Secologanin synthase OS=Catharanthus roseus OX=4058 GN=CYP72A1 PE=1 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 2.1e-76
Identity = 131/282 (46.45%), Postives = 188/282 (66.67%), Query Frame = 0


Query: 6   SIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMKDIAAMR 65
           +IR +I A +   +  W WR L+W W  PK++E+ LR+QGF GNPYR   GD+K+   M 
Sbjct: 7   TIRKAIAATIFALVMAWAWRVLDWAWFTPKRIEKRLRQQGFRGNPYRFLVGDVKESGKMH 66

Query: 66  EQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAALCLMHD 125
           ++A+S PM F++DI PR++P INHTI+ YG+NSF W G  PR+H+++P+ +K  L     
Sbjct: 67  QEALSKPMEFNNDIVPRLMPHINHTINTYGRNSFTWMGRIPRIHVMEPELIKEVLTHSSK 126

Query: 126 IQK--PTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTEMIRK 185
            QK     NPL + L  G+ + EG KW KHR+II+PAF  + LK M+PA      +M+ K
Sbjct: 127 YQKNFDVHNPLVKFLLTGVGSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHDMLTK 186

Query: 186 WEEVVSKDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQSISTV 245
           WE++  K G +E+D+ P  + L +DVIS+ AFGS+YEEG  IF  LK+L +L +  +  V
Sbjct: 187 WEKIAEKQGSHEVDIFPTFDVLTSDVISKVAFGSTYEEGGKIFRLLKELMDLTIDCMRDV 246

Query: 246 YIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASE 286
           YIPGW ++PTK N RMK+I+KE+  +L  +INKR +A+ A E
Sbjct: 247 YIPGWSYLPTKRNKRMKEINKEITDMLRFIINKRMKALKAGE 288

[Close]

BLAST of CmaCh00G000740 vs. ExPASy Swiss-Prot
Match: U5NDT8 (Secologanin synthase OS=Catharanthus roseus OX=4058 GN=SLS PE=1 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.3e-75
Identity = 130/281 (46.26%), Postives = 187/281 (66.55%), Query Frame = 0


Query: 7   IRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMKDIAAMRE 66
           IR +I A +   +  W WR L+W W  PK++E+ LR+QGF GNPYR   GD+K+   M +
Sbjct: 8   IRKAIAATIFALVMAWAWRVLDWAWFTPKRIEKRLRQQGFRGNPYRFLVGDVKESGKMHQ 67

Query: 67  QAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAALCLMHDI 126
           +A+S+PM F +DI PR++P INHTI  YG+NSF W G  PR+H+++P+ +K  L      
Sbjct: 68  EALSNPMEFDNDIVPRLMPHINHTIKTYGRNSFTWMGRIPRIHVMEPELIKEVLTHSSKY 127

Query: 127 QK--PTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTEMIRKW 186
           QK     NPL + L  G+ + EG KW KHR+II+PAF  + LK M+PA      +M+ KW
Sbjct: 128 QKNFDVHNPLVKFLLTGVGSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHDMLTKW 187

Query: 187 EEVVSKDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQSISTVY 246
           E++  K+G +E+D+ P  + L +DVIS+ AFGS+Y+EG  IF  LK+L +L +  +  VY
Sbjct: 188 EKLAEKEGSHEVDIFPTFDVLTSDVISKVAFGSTYDEGGKIFRLLKELMDLTIDCMRDVY 247

Query: 247 IPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASE 286
           IPGW ++PTK N RMK+I+KE+  +L  +INKR +A+ A E
Sbjct: 248 IPGWSYLPTKRNKRMKEINKEITDMLRFIINKRMKALKAGE 288

[Close]

BLAST of CmaCh00G000740 vs. ExPASy Swiss-Prot
Match: Q9LUC5 (Cytochrome P450 72A15 OS=Arabidopsis thaliana OX=3702 GN=CYP72A15 PE=2 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 2.8e-73
Identity = 134/288 (46.53%), Postives = 189/288 (65.62%), Query Frame = 0


Query: 1   MEI-LASIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMK 60
           MEI +AS+ +S++  VV W   W WR L W+W +PK LE  LR+QG AG PY    GD+K
Sbjct: 1   MEISVASVTISVVLAVVSW---WIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLK 60

Query: 61  DIAAMREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAA 120
               M  +A S P+  + DISPRV+P        YG+  F W GP P + I+DP+Q+K  
Sbjct: 61  KNFTMLSEARSKPLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEV 120

Query: 121 LCLMHDIQKPTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTE 180
              ++D QKP   PLA ++  GL N++G KW KHR+IINPAFH + +K+MVPA  +SC E
Sbjct: 121 FNKVYDFQKPHTFPLATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCRE 180

Query: 181 MIRKWEEVVS-KDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQ 240
           ++ +W+++VS K    E+DV P L ++ ADVISRTAFGSSY+EG+ IF    +L  L++Q
Sbjct: 181 VVGEWDQLVSDKGSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQ 240

Query: 241 SISTVYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASEA 287
           +    +IPG+ ++PTKSN RMK  ++E++ +L  ++NKR RA  A EA
Sbjct: 241 AFRKAFIPGYSYLPTKSNRRMKAAAREIQVILRGIVNKRLRAREAGEA 285

[Close]

BLAST of CmaCh00G000740 vs. ExPASy TrEMBL
Match: A0A6J1IHE1 (cytochrome P450 CYP72A219-like OS=Cucurbita maxima OX=3661 GN=LOC111473561 PE=3 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 1.0e-147
Identity = 257/287 (89.55%), Postives = 269/287 (93.73%), Query Frame = 0


Query: 1   MEILASIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMKD 60
           MEIL SIR SI AV VV LAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRL +GDMKD
Sbjct: 1   MEILGSIRDSIWAVAVVCLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLLTGDMKD 60

Query: 61  IAAMREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAAL 120
           IAAMREQAISSPMNFSHDI+PRVIPSINHT HKYGKNSF+W GPYPRVHI+DPDQLKAA 
Sbjct: 61  IAAMREQAISSPMNFSHDIAPRVIPSINHTFHKYGKNSFMWIGPYPRVHIMDPDQLKAAF 120

Query: 121 CLMHDIQKPTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTEM 180
            LMHD+QKP+INPL+RLLFDGLT+HEGPKWVKHRKIINPAFHFK LKDMVPA+CESCTEM
Sbjct: 121 SLMHDVQKPSINPLSRLLFDGLTDHEGPKWVKHRKIINPAFHFKQLKDMVPAMCESCTEM 180

Query: 181 IRKWEEVVSKDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQSI 240
           IRKWEEVVSKD YYELDVMPHL NLAADVISRTAFGSSYEEGKMIFIRLKQL NLVV++ 
Sbjct: 181 IRKWEEVVSKDEYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLVVKAT 240

Query: 241 STVYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASEAM 288
           STVYIPGWRFVPTKSN  MKKISKELKTLL+DVINKRQRAMMA EAM
Sbjct: 241 STVYIPGWRFVPTKSNNIMKKISKELKTLLMDVINKRQRAMMAGEAM 287

[Close]

BLAST of CmaCh00G000740 vs. ExPASy TrEMBL
Match: A0A6J1EYN3 (cytochrome P450 CYP72A219-like OS=Cucurbita moschata OX=3662 GN=LOC111439752 PE=3 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 1.8e-147
Identity = 263/313 (84.03%), Postives = 280/313 (89.46%), Query Frame = 0


Query: 1   MEILASIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMKD 60
           MEIL SIRVSI AV VV LAVWGWRFLNWIWLRPKKLER LR QGFAGNPYRL SGDMKD
Sbjct: 1   MEILGSIRVSIWAVAVVCLAVWGWRFLNWIWLRPKKLERSLRNQGFAGNPYRLLSGDMKD 60

Query: 61  IAAMREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAAL 120
           IAAMREQAISSPMNFSHDI+PRVIPSINHTIHKYGKNSF+W GPYPRVHI+DPDQLKAAL
Sbjct: 61  IAAMREQAISSPMNFSHDIAPRVIPSINHTIHKYGKNSFMWIGPYPRVHIMDPDQLKAAL 120

Query: 121 CLMHDIQKPTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTEM 180
            LMHD+QKP+INPL+RLLFDGLT+HEGPKWVKHRKIINPAFHFKHLK MVPA+CESCTEM
Sbjct: 121 SLMHDVQKPSINPLSRLLFDGLTDHEGPKWVKHRKIINPAFHFKHLKGMVPAMCESCTEM 180

Query: 181 IRKWEEVVSKDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQSI 240
           IRKWEEVVSKDGYYELDVMPHL NLAADVISRTAFGSSYEEGKMIFIRLKQL NLVV++ 
Sbjct: 181 IRKWEEVVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLVVKAT 240

Query: 241 STVYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASEAMQVSIKHPLRIKFE 300
           STVYIPGWRFVPTKSN RMKKISKEL+ LLLDVINKRQRAMMA  AM   +   L I  +
Sbjct: 241 STVYIPGWRFVPTKSNNRMKKISKELRDLLLDVINKRQRAMMAGAAMHNDL---LSILLQ 300

Query: 301 RNSRARKQRCRNE 314
            N +  ++   N+
Sbjct: 301 SNLKEIREHGNND 310

[Close]

BLAST of CmaCh00G000740 vs. ExPASy TrEMBL
Match: A0A5D3CEG3 (Cytochrome P450 CYP72A219-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003160 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 3.5e-111
Identity = 199/295 (67.46%), Postives = 236/295 (80.00%), Query Frame = 0


Query: 14  VVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMKDIAAMREQAISSPM 73
           V +V L   GWRFLNWIWL PKKLE+CLR+QGFAGNPYRL+SGD+ D+ AM E+A S PM
Sbjct: 11  VFMVCLLRCGWRFLNWIWLTPKKLEKCLREQGFAGNPYRLYSGDLNDVVAMEEEAKSKPM 70

Query: 74  NFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAALCLMHDIQKPTINP 133
           NFSHDI PRVIPS++HTI KYGKNSF+W GP PRV I+DP+QLKAAL L +D QKPT NP
Sbjct: 71  NFSHDIVPRVIPSMHHTIKKYGKNSFMWLGPNPRVLIMDPEQLKAALSLYNDFQKPTTNP 130

Query: 134 LARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTEMIRKWEEVVSKDGY 193
           L RLLFDGL NHEG KWVKHRKIIN AFHF+ LKDMVP + ESC EMI KWE++ SKDG 
Sbjct: 131 LVRLLFDGLINHEGEKWVKHRKIINHAFHFEKLKDMVPTMFESCNEMISKWEKMTSKDGS 190

Query: 194 YELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQSISTVYIPGWRFVPT 253
            ELDV+P+L++L ADVISRTAFGS+YEEGK+IF   K+L  LV+Q+ +TVY+PGWRF+PT
Sbjct: 191 CELDVVPYLQSLTADVISRTAFGSTYEEGKIIFNHFKELIRLVLQASNTVYLPGWRFLPT 250

Query: 254 KSNTRMKKISKELKTLLLDVINKRQRAMMASEAMQVSIKHPLRIKFERNSRARKQ 309
           KSN RMKKI+KE+KT+L+++I+KRQR M   E M       L I  E NS   K+
Sbjct: 251 KSNNRMKKITKEIKTILMNIIDKRQRTMKEDETMHSD--DLLGILLESNSNEIKE 303

[Close]

BLAST of CmaCh00G000740 vs. ExPASy TrEMBL
Match: A0A1S3BQL9 (cytochrome P450 CYP72A219-like OS=Cucumis melo OX=3656 GN=LOC103492242 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 3.5e-111
Identity = 199/295 (67.46%), Postives = 236/295 (80.00%), Query Frame = 0


Query: 14  VVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMKDIAAMREQAISSPM 73
           V +V L   GWRFLNWIWL PKKLE+CLR+QGFAGNPYRL+SGD+ D+ AM E+A S PM
Sbjct: 11  VFMVCLLRCGWRFLNWIWLTPKKLEKCLREQGFAGNPYRLYSGDLNDVVAMEEEAKSKPM 70

Query: 74  NFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAALCLMHDIQKPTINP 133
           NFSHDI PRVIPS++HTI KYGKNSF+W GP PRV I+DP+QLKAAL L +D QKPT NP
Sbjct: 71  NFSHDIVPRVIPSMHHTIKKYGKNSFMWLGPNPRVLIMDPEQLKAALSLYNDFQKPTTNP 130

Query: 134 LARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTEMIRKWEEVVSKDGY 193
           L RLLFDGL NHEG KWVKHRKIIN AFHF+ LKDMVP + ESC EMI KWE++ SKDG 
Sbjct: 131 LVRLLFDGLINHEGEKWVKHRKIINHAFHFEKLKDMVPTMFESCNEMISKWEKMTSKDGS 190

Query: 194 YELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQSISTVYIPGWRFVPT 253
            ELDV+P+L++L ADVISRTAFGS+YEEGK+IF   K+L  LV+Q+ +TVY+PGWRF+PT
Sbjct: 191 CELDVVPYLQSLTADVISRTAFGSTYEEGKIIFNHFKELIRLVLQASNTVYLPGWRFLPT 250

Query: 254 KSNTRMKKISKELKTLLLDVINKRQRAMMASEAMQVSIKHPLRIKFERNSRARKQ 309
           KSN RMKKI+KE+KT+L+++I+KRQR M   E M       L I  E NS   K+
Sbjct: 251 KSNNRMKKITKEIKTILMNIIDKRQRTMKEDETMHSD--DLLGILLESNSNEIKE 303

[Close]

BLAST of CmaCh00G000740 vs. ExPASy TrEMBL
Match: A0A1S3BT76 (cytochrome P450 CYP72A219-like OS=Cucumis melo OX=3656 GN=LOC103493471 PE=3 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 3.6e-100
Identity = 186/316 (58.86%), Postives = 235/316 (74.37%), Query Frame = 0


Query: 3   ILASIRVSILAVVVVWLA---VWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMK 62
           +L +I   ++ V+VV L+   + GW+ ++WIW RPKKLE+ LR+QGF GN YR+  GD+K
Sbjct: 4   VLDTISTWLIVVIVVLLSSLLLLGWKLVDWIWFRPKKLEKLLRQQGFTGNSYRILYGDLK 63

Query: 63  DIAAMREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAA 122
           + AAMR+QAIS PMNFS+ I+PRVIPS++HTI  YGKNSF+W GP PRVHI+DP+QLK  
Sbjct: 64  ERAAMRDQAISKPMNFSNHIAPRVIPSVHHTIQHYGKNSFMWIGPIPRVHIMDPEQLKTV 123

Query: 123 LCLMHDIQKPT--INPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESC 182
             L++D QKPT  +NPLA+LL DGL NHEG KWVKHRKIINPAFH + LKDMVPA   SC
Sbjct: 124 FSLINDYQKPTASLNPLAKLLADGLLNHEGHKWVKHRKIINPAFHLEKLKDMVPAFYHSC 183

Query: 183 TEMIRKWEEVVSKDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVV 242
            EMI KWE +VS  G  ELDVMP+L+N+ ADVISRTAFGSSYE+GK IF    +L  +V+
Sbjct: 184 DEMISKWESMVSVQGSCELDVMPYLQNMTADVISRTAFGSSYEKGKKIFKLQTELAEMVI 243

Query: 243 QSISTVYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASEAMQVSIKHPLRI 302
           +S   +YIPGWRF+PTKSN +MK ISKE+ TL++ +IN+R+ +M A EA Q  +   L I
Sbjct: 244 RSTLGIYIPGWRFLPTKSNNKMKVISKEISTLIMGIINERETSMKAGEATQTDL---LSI 303

Query: 303 KFERNSRARKQRCRNE 314
             E N    KQ   N+
Sbjct: 304 LMESNLNEIKQHGNNK 316

[Close]

BLAST of CmaCh00G000740 vs. NCBI nr
Match: XP_023515512.1 (cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 549.7 bits (1415), Expect = 1.7e-152
Identity = 270/313 (86.26%), Postives = 287/313 (91.69%), Query Frame = 0


Query: 1   MEILASIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMKD 60
           MEIL SIRVSI AV VV LAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRL +GDMKD
Sbjct: 1   MEILGSIRVSIWAVAVVCLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLLTGDMKD 60

Query: 61  IAAMREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAAL 120
           IA MREQAISSPMNFSHDI+PRVIPSINHTIHKYGKNSF+W GPYPRVHI+DPDQLKAAL
Sbjct: 61  IATMREQAISSPMNFSHDIAPRVIPSINHTIHKYGKNSFMWVGPYPRVHIMDPDQLKAAL 120

Query: 121 CLMHDIQKPTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTEM 180
            LMHDIQKPTINPLARLLFDGLT HEGPKWVKHRKI+NPAFHFKHLKDMVPA+CESCTEM
Sbjct: 121 SLMHDIQKPTINPLARLLFDGLTTHEGPKWVKHRKIMNPAFHFKHLKDMVPAVCESCTEM 180

Query: 181 IRKWEEVVSKDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQSI 240
           IRKWEEVVS++G+YELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLK+LTNLVVQSI
Sbjct: 181 IRKWEEVVSEEGFYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKELTNLVVQSI 240

Query: 241 STVYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASEAMQVSIKHPLRIKFE 300
           STVYI GWRFVPTKSNTRMKKISKEL TLLLDVINKRQRAMMASEAM   +   L I  +
Sbjct: 241 STVYISGWRFVPTKSNTRMKKISKELNTLLLDVINKRQRAMMASEAMPTDL---LSILLQ 300

Query: 301 RNSRARKQRCRNE 314
            N +  +++  N+
Sbjct: 301 SNLKKIREQGNND 310

[Close]

BLAST of CmaCh00G000740 vs. NCBI nr
Match: XP_023520477.1 (cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 540.8 bits (1392), Expect = 7.9e-150
Identity = 262/313 (83.71%), Postives = 285/313 (91.05%), Query Frame = 0


Query: 1   MEILASIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMKD 60
           MEIL SIRVSILAV+VVWLAVWGWR+LNWIWLRPKKLERCLRKQGFAGNPYRL +GD+KD
Sbjct: 1   MEILGSIRVSILAVIVVWLAVWGWRYLNWIWLRPKKLERCLRKQGFAGNPYRLLTGDIKD 60

Query: 61  IAAMREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAAL 120
           IAAMREQAISSPMNFSHDI+PRVIPS+NHTIH YGKNSF+W GPYPRVHI+DPDQLKAA 
Sbjct: 61  IAAMREQAISSPMNFSHDIAPRVIPSMNHTIHTYGKNSFMWIGPYPRVHIMDPDQLKAAF 120

Query: 121 CLMHDIQKPTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTEM 180
            LMHDIQKP+INPL+RLLFDGL +HEGPKWVKHRKIINPAFHFKHLKDMVPA+CESCTEM
Sbjct: 121 SLMHDIQKPSINPLSRLLFDGLADHEGPKWVKHRKIINPAFHFKHLKDMVPAMCESCTEM 180

Query: 181 IRKWEEVVSKDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQSI 240
           IRKWEE+VSKDGYYELDVMPHL NLAADVISRTAFGSSYEEGKMIFIRLKQL NL+V+ +
Sbjct: 181 IRKWEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLIVKVV 240

Query: 241 STVYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASEAMQVSIKHPLRIKFE 300
           STVYIPGWRFVPTKSN RMKKISKELKTLLL+VINKRQRAMMA EAM   +   L I  +
Sbjct: 241 STVYIPGWRFVPTKSNNRMKKISKELKTLLLNVINKRQRAMMAGEAMHNDL---LSILLQ 300

Query: 301 RNSRARKQRCRNE 314
            N +  ++   N+
Sbjct: 301 SNLKEIREHENND 310

[Close]

BLAST of CmaCh00G000740 vs. NCBI nr
Match: XP_022974803.1 (cytochrome P450 CYP72A219-like [Cucurbita maxima])

HSP 1 Score: 532.7 bits (1371), Expect = 2.2e-147
Identity = 257/287 (89.55%), Postives = 269/287 (93.73%), Query Frame = 0


Query: 1   MEILASIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMKD 60
           MEIL SIR SI AV VV LAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRL +GDMKD
Sbjct: 1   MEILGSIRDSIWAVAVVCLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLLTGDMKD 60

Query: 61  IAAMREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAAL 120
           IAAMREQAISSPMNFSHDI+PRVIPSINHT HKYGKNSF+W GPYPRVHI+DPDQLKAA 
Sbjct: 61  IAAMREQAISSPMNFSHDIAPRVIPSINHTFHKYGKNSFMWIGPYPRVHIMDPDQLKAAF 120

Query: 121 CLMHDIQKPTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTEM 180
            LMHD+QKP+INPL+RLLFDGLT+HEGPKWVKHRKIINPAFHFK LKDMVPA+CESCTEM
Sbjct: 121 SLMHDVQKPSINPLSRLLFDGLTDHEGPKWVKHRKIINPAFHFKQLKDMVPAMCESCTEM 180

Query: 181 IRKWEEVVSKDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQSI 240
           IRKWEEVVSKD YYELDVMPHL NLAADVISRTAFGSSYEEGKMIFIRLKQL NLVV++ 
Sbjct: 181 IRKWEEVVSKDEYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLVVKAT 240

Query: 241 STVYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASEAM 288
           STVYIPGWRFVPTKSN  MKKISKELKTLL+DVINKRQRAMMA EAM
Sbjct: 241 STVYIPGWRFVPTKSNNIMKKISKELKTLLMDVINKRQRAMMAGEAM 287

[Close]

BLAST of CmaCh00G000740 vs. NCBI nr
Match: XP_022933047.1 (cytochrome P450 CYP72A219-like [Cucurbita moschata])

HSP 1 Score: 531.9 bits (1369), Expect = 3.7e-147
Identity = 263/313 (84.03%), Postives = 280/313 (89.46%), Query Frame = 0


Query: 1   MEILASIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMKD 60
           MEIL SIRVSI AV VV LAVWGWRFLNWIWLRPKKLER LR QGFAGNPYRL SGDMKD
Sbjct: 1   MEILGSIRVSIWAVAVVCLAVWGWRFLNWIWLRPKKLERSLRNQGFAGNPYRLLSGDMKD 60

Query: 61  IAAMREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAAL 120
           IAAMREQAISSPMNFSHDI+PRVIPSINHTIHKYGKNSF+W GPYPRVHI+DPDQLKAAL
Sbjct: 61  IAAMREQAISSPMNFSHDIAPRVIPSINHTIHKYGKNSFMWIGPYPRVHIMDPDQLKAAL 120

Query: 121 CLMHDIQKPTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTEM 180
            LMHD+QKP+INPL+RLLFDGLT+HEGPKWVKHRKIINPAFHFKHLK MVPA+CESCTEM
Sbjct: 121 SLMHDVQKPSINPLSRLLFDGLTDHEGPKWVKHRKIINPAFHFKHLKGMVPAMCESCTEM 180

Query: 181 IRKWEEVVSKDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQSI 240
           IRKWEEVVSKDGYYELDVMPHL NLAADVISRTAFGSSYEEGKMIFIRLKQL NLVV++ 
Sbjct: 181 IRKWEEVVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLVVKAT 240

Query: 241 STVYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASEAMQVSIKHPLRIKFE 300
           STVYIPGWRFVPTKSN RMKKISKEL+ LLLDVINKRQRAMMA  AM   +   L I  +
Sbjct: 241 STVYIPGWRFVPTKSNNRMKKISKELRDLLLDVINKRQRAMMAGAAMHNDL---LSILLQ 300

Query: 301 RNSRARKQRCRNE 314
            N +  ++   N+
Sbjct: 301 SNLKEIREHGNND 310

[Close]

BLAST of CmaCh00G000740 vs. NCBI nr
Match: XP_023516285.1 (cytochrome P450 CYP72A219-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023516286.1 cytochrome P450 CYP72A219-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023516287.1 cytochrome P450 CYP72A219-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 528.9 bits (1361), Expect = 3.1e-146
Identity = 261/313 (83.39%), Postives = 278/313 (88.82%), Query Frame = 0


Query: 1   MEILASIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMKD 60
           MEIL SIRVSI AV VV LAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRL +GDMKD
Sbjct: 1   MEILGSIRVSIWAVAVVCLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLLTGDMKD 60

Query: 61  IAAMREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAAL 120
           IA MREQAISSPMNFSHDI+PRVIPSINHTIHKYGKNSF+W GPYP VHI+DPDQLKAAL
Sbjct: 61  IATMREQAISSPMNFSHDIAPRVIPSINHTIHKYGKNSFMWIGPYPSVHIMDPDQLKAAL 120

Query: 121 CLMHDIQKPTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTEM 180
            LMHD++KP+INPL RLLFDGLT+HEGPKWVKHRKIINPAFHFK LKDMVPA+CESCTEM
Sbjct: 121 SLMHDVRKPSINPLQRLLFDGLTDHEGPKWVKHRKIINPAFHFKQLKDMVPAMCESCTEM 180

Query: 181 IRKWEEVVSKDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQSI 240
           IRKWEE VSKDGYYELDVMPHL NLAADVISRTAFGSSYEEGKMIFIRLKQL NL V++ 
Sbjct: 181 IRKWEEGVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLFVKAT 240

Query: 241 STVYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASEAMQVSIKHPLRIKFE 300
           STVYIPGWRFVPTKSN RMKKISKELK LLLDVINKRQRAMMA EAM   +   L I  +
Sbjct: 241 STVYIPGWRFVPTKSNNRMKKISKELKALLLDVINKRQRAMMAGEAMHNDL---LSILLQ 300

Query: 301 RNSRARKQRCRNE 314
            N +  ++   N+
Sbjct: 301 SNLKEIQEHGNND 310

[Close]

BLAST of CmaCh00G000740 vs. TAIR 10
Match: AT3G14690.1 (cytochrome P450, family 72, subfamily A, polypeptide 15 )

HSP 1 Score: 276.9 bits (707), Expect = 2.0e-74
Identity = 134/288 (46.53%), Postives = 189/288 (65.62%), Query Frame = 0


Query: 1   MEI-LASIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMK 60
           MEI +AS+ +S++  VV W   W WR L W+W +PK LE  LR+QG AG PY    GD+K
Sbjct: 1   MEISVASVTISVVLAVVSW---WIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLK 60

Query: 61  DIAAMREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAA 120
               M  +A S P+  + DISPRV+P        YG+  F W GP P + I+DP+Q+K  
Sbjct: 61  KNFTMLSEARSKPLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEV 120

Query: 121 LCLMHDIQKPTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTE 180
              ++D QKP   PLA ++  GL N++G KW KHR+IINPAFH + +K+MVPA  +SC E
Sbjct: 121 FNKVYDFQKPHTFPLATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCRE 180

Query: 181 MIRKWEEVVS-KDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQ 240
           ++ +W+++VS K    E+DV P L ++ ADVISRTAFGSSY+EG+ IF    +L  L++Q
Sbjct: 181 VVGEWDQLVSDKGSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQ 240

Query: 241 SISTVYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASEA 287
           +    +IPG+ ++PTKSN RMK  ++E++ +L  ++NKR RA  A EA
Sbjct: 241 AFRKAFIPGYSYLPTKSNRRMKAAAREIQVILRGIVNKRLRAREAGEA 285

[Close]

BLAST of CmaCh00G000740 vs. TAIR 10
Match: AT3G14620.1 (cytochrome P450, family 72, subfamily A, polypeptide 8 )

HSP 1 Score: 271.2 bits (692), Expect = 1.1e-72
Identity = 137/301 (45.51%), Postives = 198/301 (65.78%), Query Frame = 0


Query: 4   LASIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMKDIAA 63
           ++++ V+   VVV  + VW W+ LN  WLRPKK E  L++QG +G P+    GD+K  A+
Sbjct: 6   ISAVAVAAAVVVVTTVTVWIWKGLNVAWLRPKKNEAYLKRQGLSGTPFTFLVGDIKREAS 65

Query: 64  MREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAALCLM 123
           M EQ  S P+N + D + RV+P I  T+  +GK S++W GP   V +  P+ +K  L  +
Sbjct: 66  MVEQEKSRPINLTDDYTHRVMPLIQQTVKDHGKTSYMWMGPIASVIVTKPEHIKDVLNRV 125

Query: 124 HDIQKPTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTEMIRK 183
           +D  KP ++P+  L   G+  +EG KW KHRKIINP+FH + LK M+PA  ESC+EMI K
Sbjct: 126 YDFPKPPVHPIVELFATGVALYEGEKWSKHRKIINPSFHLEKLKIMIPAFYESCSEMISK 185

Query: 184 WEEVVSKDG-YYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQSIST 243
           WE++V++ G   E+DV P+L +L +DVISRTAFGSSYEEGK IF   ++    V++++  
Sbjct: 186 WEKLVTEQGSSNEIDVWPYLGDLTSDVISRTAFGSSYEEGKRIFELQEEQGRRVLKALEL 245

Query: 244 VYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASEAMQVSIKHPLRIKFERN 303
            +IPG RF+PTK+N RM++I+KE+K+ L ++I KRQR M   EA +  +   L I  E N
Sbjct: 246 AFIPGMRFLPTKNNLRMRQINKEVKSRLREIIMKRQRGMDTGEAPKNDL---LGILLESN 303

[Close]

BLAST of CmaCh00G000740 vs. TAIR 10
Match: AT3G14650.1 (cytochrome P450, family 72, subfamily A, polypeptide 11 )

HSP 1 Score: 268.5 bits (685), Expect = 7.1e-72
Identity = 131/288 (45.49%), Postives = 191/288 (66.32%), Query Frame = 0


Query: 1   MEI-LASIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMK 60
           MEI +AS+ VS+  VVV W   W WR L W+W +PK LE  LR+QG AG PY    GD+K
Sbjct: 1   MEISVASVTVSVAVVVVSW---WVWRTLQWVWFKPKMLESYLRRQGLAGTPYTPLVGDLK 60

Query: 61  DIAAMREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAA 120
              +MR +A S P+N + DI+PR++P     +  +G+  F W G  P + I+DP+Q+   
Sbjct: 61  KNFSMRAEARSKPINLTDDITPRIVPYPLQMLKTHGRTFFTWFGAIPTITIMDPEQITEV 120

Query: 121 LCLMHDIQKPTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTE 180
           L  ++D QK    PL RL+  G+ +++G KW KHR+IINPAFH + +K+MVPA  +SC+E
Sbjct: 121 LNKVYDFQKAHTFPLGRLIATGVLSYDGDKWAKHRRIINPAFHLEKIKNMVPAFHQSCSE 180

Query: 181 MIRKWEEVVS-KDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQ 240
           ++ KW+++VS K+   E+DV P L ++ ADVISRTAFGSS  EG+ IF    +L  L++Q
Sbjct: 181 IVCKWDKLVSDKESSCEVDVWPGLVSMTADVISRTAFGSSCVEGQRIFELQAELAQLIIQ 240

Query: 241 SISTVYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASEA 287
           ++   +IPG+ ++PTK N RMK  ++E++ +L  ++NKR RA  A EA
Sbjct: 241 TVRKAFIPGYSYLPTKGNRRMKAKAREIQVILRGIVNKRLRAREAGEA 285

[Close]

BLAST of CmaCh00G000740 vs. TAIR 10
Match: AT3G14660.1 (cytochrome P450, family 72, subfamily A, polypeptide 13 )

HSP 1 Score: 268.1 bits (684), Expect = 9.2e-72
Identity = 132/288 (45.83%), Postives = 190/288 (65.97%), Query Frame = 0


Query: 1   MEI-LASIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMK 60
           MEI +AS+ VS+  VVV W   W WR L  +WL+PK LE  LR+QG AG PY    GD+K
Sbjct: 1   MEISVASVTVSVAVVVVSW---WVWRTLQRVWLKPKMLESYLRRQGLAGTPYTPLVGDLK 60

Query: 61  DIAAMREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAA 120
              +M  +A S P+N + DI+PR++P     +  +G+  F W GP P + I+DP+Q+K  
Sbjct: 61  RNFSMLAEARSKPINLTDDITPRIVPYPLQMLKTHGRTFFTWFGPIPTITIMDPEQIKEV 120

Query: 121 LCLMHDIQKPTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTE 180
              ++D QK    PL RL+  GL +++G KW KHR+IINPAFH + +K+MVPA  +SC+E
Sbjct: 121 FNKVYDFQKAHTFPLGRLIAAGLVSYDGDKWTKHRRIINPAFHLEKIKNMVPAFHQSCSE 180

Query: 181 MIRKWEEVVS-KDGYYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVQ 240
           ++ +W+++V+ K    E+D+ P L ++ ADVISRTAFGSSY+EG+ IF    +L  L++Q
Sbjct: 181 IVGEWDKLVTDKQSSCEVDIWPWLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQ 240

Query: 241 SISTVYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASEA 287
           +     IPG+R+ PTK N RMK  ++E+K +L  ++NKR RA  A EA
Sbjct: 241 AFRKAIIPGYRYFPTKGNRRMKAAAREIKFILRGIVNKRLRAREAGEA 285

[Close]

BLAST of CmaCh00G000740 vs. TAIR 10
Match: AT3G14640.1 (cytochrome P450, family 72, subfamily A, polypeptide 10 )

HSP 1 Score: 262.7 bits (670), Expect = 3.9e-70
Identity = 129/289 (44.64%), Postives = 188/289 (65.05%), Query Frame = 0


Query: 1   MEI-LASIRVSILAVVVVWLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLFSGDMK 60
           MEI +A + VS++ VVV W   W WR L W+W +PK LE  LR+QG AG PY    GD+K
Sbjct: 1   MEISVACVTVSVVVVVVSW---WVWRTLQWVWFKPKMLESYLRRQGLAGTPYTPLIGDLK 60

Query: 61  DIAAMREQAISSPMNFSHDISPRVIPSINHTIHKYGKNSFIWTGPYPRVHIVDPDQLKAA 120
               M  +A S P+  + DI+PRV+P     +  +G+  F W GP P + I+DP+ +K  
Sbjct: 61  RNVNMLTEATSKPIKLTEDITPRVLPHPFQMLKTHGRTFFTWLGPKPTITIMDPELIKEV 120

Query: 121 LCLMHDIQKPTINPLARLLFDGLTNHEGPKWVKHRKIINPAFHFKHLKDMVPAICESCTE 180
              ++D  K     L RL+  G+ N++G KW KHR+IINPAFH + +K+MVPA  +SC++
Sbjct: 121 FNKVYDYPKAQTFLLGRLIATGIINYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCSD 180

Query: 181 MIRKWEEVVSKDG--YYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVV 240
           ++ +W ++VS  G    E+DV P L ++  DVISRTAFGSSY+EG+ IF    +L +L++
Sbjct: 181 VVGEWSKLVSDKGSSSCEVDVWPWLVSMTGDVISRTAFGSSYKEGQRIFELQAELVHLIL 240

Query: 241 QSISTVYIPGWRFVPTKSNTRMKKISKELKTLLLDVINKRQRAMMASEA 287
           Q+   VYIPG+R++PTKSN RMK  ++E++ +L  ++NKR RA  A +A
Sbjct: 241 QAFWKVYIPGYRYLPTKSNRRMKAAAREIQVILKGIVNKRLRAREAGKA 286

The following BLAST results are available for this feature:

BLAST of CmaCh00G000740 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
H2DH21	2.9e-86	52.27	Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]
A0A0S2IHL2	1.3e-81	48.76	Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1	[more]
Q05047	2.1e-76	46.45	Secologanin synthase OS=Catharanthus roseus OX=4058 GN=CYP72A1 PE=1 SV=1	[more]
U5NDT8	1.3e-75	46.26	Secologanin synthase OS=Catharanthus roseus OX=4058 GN=SLS PE=1 SV=1	[more]
Q9LUC5	2.8e-73	46.53	Cytochrome P450 72A15 OS=Arabidopsis thaliana OX=3702 GN=CYP72A15 PE=2 SV=1	[more]

BLAST of CmaCh00G000740 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IHE1	1.0e-147	89.55	cytochrome P450 CYP72A219-like OS=Cucurbita maxima OX=3661 GN=LOC111473561 PE=3 ...	[more]
A0A6J1EYN3	1.8e-147	84.03	cytochrome P450 CYP72A219-like OS=Cucurbita moschata OX=3662 GN=LOC111439752 PE=...	[more]
A0A5D3CEG3	3.5e-111	67.46	Cytochrome P450 CYP72A219-like protein OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BQL9	3.5e-111	67.46	cytochrome P450 CYP72A219-like OS=Cucumis melo OX=3656 GN=LOC103492242 PE=3 SV=1	[more]
A0A1S3BT76	3.6e-100	58.86	cytochrome P450 CYP72A219-like OS=Cucumis melo OX=3656 GN=LOC103493471 PE=3 SV=1	[more]

BLAST of CmaCh00G000740 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023515512.1	1.7e-152	86.26	cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo]	[more]
XP_023520477.1	7.9e-150	83.71	cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo]	[more]
XP_022974803.1	2.2e-147	89.55	cytochrome P450 CYP72A219-like [Cucurbita maxima]	[more]
XP_022933047.1	3.7e-147	84.03	cytochrome P450 CYP72A219-like [Cucurbita moschata]	[more]
XP_023516285.1	3.1e-146	83.39	cytochrome P450 CYP72A219-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_02351...	[more]

BLAST of CmaCh00G000740 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14690.1	2.0e-74	46.53	cytochrome P450, family 72, subfamily A, polypeptide 15	[more]
AT3G14620.1	1.1e-72	45.51	cytochrome P450, family 72, subfamily A, polypeptide 8	[more]
AT3G14650.1	7.1e-72	45.49	cytochrome P450, family 72, subfamily A, polypeptide 11	[more]
AT3G14660.1	9.2e-72	45.83	cytochrome P450, family 72, subfamily A, polypeptide 13	[more]
AT3G14640.1	3.9e-70	44.64	cytochrome P450, family 72, subfamily A, polypeptide 10	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 61..310 e-value: 6.0E-32 score: 112.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 80..285
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 91..283 e-value: 8.2E-22 score: 77.6
None	No IPR available	PANTHER	PTHR24282:SF140	CYTOCHROME	coord: 11..286
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 11..286

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh00G000740.1	CmaCh00G000740.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen