CmUC06G123930 (gene) Watermelon (USVL531) v1

Overview
NameCmUC06G123930
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
DescriptionFlavonoid 3' hydroxylase
LocationCmU531Chr06: 27459809 .. 27464510 (-)
RNA-Seq ExpressionCmUC06G123930
SyntenyCmUC06G123930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTGAATCAATAACCCAAAAATTTATATATATTTAATGGACGAAATTACCCTCATCGTCGCCACCGTAGTTACCTTGGCCTCCGCCATCCTCCTCTACCGTGCTTTCTTCCTCCGCCGCAGATTCCGCCTCCCGCCTGGTCCAAAACCATGGCCGATCGTAGGAAACATGCCGCAAATCGGCGGAGCGCCTCTCCATTCCCTCCTCGCCACCATGGCCGGCGAGTACGGACCTCTGATGTACCTCCGATTAGGCTCCGTTGACATGGTCGTGGCGGCGTCGGCGGCAGTAGCGGAGCAACTCTTGAAGATACACGATACTAATTTCCTTAGCCGCCCGCCGAACGCCGGCGCAAAATACATTGCTTATAATTATCAGGATATGGTTTTTGCGCCGTACGGTCCACGGTGGCGATTACTCCGGAAAATCAGCGCTGTCCATCTCTTCTCCTCAAAAGCCCTAGATGATCATCGCCGCATACGCGAGGTTCGCCCTATACATATATATATTTTTCTTTCTATTTATATGCATGCCTGATTTTTACCCTAATTTTCTTCTTCTCAGAAAATAGGTTTTCGTGTTTGGAAAATGATAATAATATGATTTTTTTTTTTTTTAATTTGGTCTCTATACTCCTAAATTTTAGAAAAAACTAATAACAAAATATCTGATAGACTATCACGACCTATCTCAATCTATGATCATATGGTTATCACCCTTCTTGAAATATAGTAATGAATAACAAATTTGAGTATACAAAGATAAATTGAACCACAACTAAAAGTATAAACACTAAACGCGCATAATATAACACCACTCTAAAGTGAACTAGAAATTCTAGTTCACTCAAATTTTTGGACTAAACTTATTGAGTAAGCCGATGTTAATCTACGAAGTTGGATACTTAAAATTTAGGGATTTCATAGAAAATTAAAAATAACACTTTAAATGTACTATCTCATCTTTCTAGTATCGAGTGAGACCTATGGTATGCACCTAAAATTTTCCATTATCTACTTTCCTACGTGGGGAAACCTTACCAAGCCAGTTGTCCGTTCCTGCCGACGCAATAGTTCTCACTTTGCTGGGCTCTCAAAATTCACAACAGTACAAAATCACGGTGTTCTTTTTCTTTTTTCTTTTTTTCTTTTTATTATTTATTTTTCCTTATTAGTTTAATTAACAGTTATGAGAGTTGGGTAAGCCATTTGACTTCGTCCAATTTCTTTAACAAGAAAAAAAAAAATAATAATAATAAATTTTAGTTTATCACCTCACTACGCACGCTTTGTTGAATTGATCATTAATGTGATTTCTTAGGTTGCGTGCTAACTAACCGCTTTTAACTGAGTTGGAATACCAATTTTTAAATATTTAATTTAAAAAATAAGTTATTAAAAAAAATATGTTAAAGTGTATTCTAAATAATTTTAATCAAAATAGTTTAAATAAAAATGAATTTTTTGATAATTTTTTTAAATAATCCAAATGGATCTTTAGTTTATTTATTAAAAGTCAACTTTCTTGAGACTCTACTTATATCACTTCATATAGGTATATTAATGAAAAATTAATGGATTAACTCAAAAACACATGAATTTCGATGTTGTACTACAAAACCATTATATTAGATAACATGATTATGAGTATGAAAATAATAATGGAACATGCGACTTATGTAATCAAGTTCTGTCTTGCACGACAAAACAATTATAAGATAAGGTAAGTATGAGTATGAAAAGATGAAGGAACATTTAATTCTTTTCATTGACTTATGTGAAGACTAAACTAACTTTGAGAAACCGACAGGAGAGTGAGTACGTGTAAGTGTATAAACCAAATGCATTTTATAGTTATTATTTTTTCTTTTTTGTACTTATTCTCTATCAAATTAACCATAGATCTACCTGGGGAAGAACAATTGTGTGTAAATTTCGTTTCTTGATAGAATTTAGTGAGAAAATTATAAAGAAAAAAAAAATAAAATCATATAGTTTTCTGATATAATTTTTAAACCTAGTACAAGTAAAGTAAGGTTTTTATTTGTTTATTTATTTGTTTATTATTTTTTTTCATCACTCTACCTCTAGACTCTAGAGTTAGTCCTTTTCTAAAGAGAGAAAAAATAATAATAATAATAATCCTGTTTTTGTTATAACTGTGGAATTTTTTTAATCATACCAGAAAATGTGATATATTTAAATATATCTAATATGAATTTACATAAATAGCATAATATAAAGATTAACATGTACTTTAATATAACATGAAATTTAAAATTTTACAAAAATAAAGTATAGCTTAAATTCAACCCAAGTTCAAATTCAATATAACCATGTTAAAAAGAAAATTCTAATGTTTTTTTTTTTAATAAGTAGAAAATTATAATTTATGAAAACAATAAGAAAATATGTGACTTCTTTTGATAATAGATATTAAATATATGCTCTTCAATAAAAAAGAAAAAATATATCAATAATTTTCAATATAGTAGAGATAATTAAAATTTATATCCGAGTACAGGAGGAGATAGCGATGCTAGTACAGGCTCTTGCAAGGTCCGGCGAAGCTCCGGCGACCTTGGGGAGCTTGCTCACCGTGTGCACTGCGAATGCGCTCGGACGGACAATGCTCGGCCGACGGGTTTTCGGCGATGGCAGCGGCGGCAACGATCCAGAGGCGAACCGATTTAAGGAAATGGTCGAGCAAGTGATGGTGCTGGCCGGAAAGTTCAACCCCGGCGACTTTTTCCCGTCGCTTGAGTGGCTTGATTTGTTGGGTGTGGCAAGTGAGATGAAGAAGGTCCACAAATGGTTCGACGATTTTTTGACCAAGATCTTGGAGGAGCACCGGAATTTATTGGCCGCAGGCCGTGGCGGCGGCGGCGACCACCACCAAGACTTGTTGAGTACTCTTCTGTCGTCAAAGGATGAAGCTGAGGATGAGAATGAGAAACTTAATGACACTGAGATCAAGGCTTTGTTGTTGGTATTATTTCTCTCACTTCTCATATAGATCTAGTTACACTATTAAGAAAAAACATTTCAAAACTATCATTAAAAAAAAAAAACCTTTCAAAACTACCGTAGAGTAAGTAGAGTAAAAAGACTTATGAAGAATATGCTGCCTAACCTAAGACTTAAAACAAAAATTCATATAGTTATAATGTGTCGGCAAAAGTTTAATAACAAAAATATGAAAAGAAAAAGAGACGCATACGAAACCTCTTTAATAAGAATCGCTAACTCTATTCTCATTTCGGTAAGGTTTTTGTCTAAAACTTGAAGCATTTTCACTCTCATAATAATTTTAAAATATTTATGAACTATCAAGAGTAATAGTGTAAATATTTATGAAATGTCGAGCAATATTGTAATTTTTAGAATAATATTGATATTTTTTAAATAATGATCAAAATTATTTAATTTTTATAATTAAGCCTGTCTAAAAATAACTTAAAGAAAAAAATGATTCGAAGGGTAGTGTCAAATGTCAATTAACATGTACAATATATTATTAAAAAGATGTACAACACAAAAATCACAAACATTTTTTTTTTTAGATATTTAGAAAAAAATACAGGTTAATTTGTTATTATATTAAGTTCAGGTACTTGTCAACATGACTATACTTTAATAATCAATATATCTATTATTTATTTATTTTTGAATAATATGTAGGTTCCAATATTACCAAAAATGGCTGTGTAATTTACCTAGGTTAAAAGTGTGAGTAATTAATTTGCCATTCAAATCAAATTAAAGGAACAACAAAAGTTATATACATTGAACGAGAATACATAAATTGCAATAAATTTCATTAAAAGGAAAGATTAGATTGCCAAACCCTTTCATTATAGAATTACAATCTAAATCAAATCCATCTGGTGGTATTATTATTATTATTTCATGCAGAATATGTTTACCGCTGGAACCGACACTTCAGCAAGCACAGTGGAATGGGCCTTGACAGAGCTCATCAGACATCCCAAAATGATGGCCCAGGCCCAACAAGAATTGGACTCCGCAGTGGGCCGTGATCGGGCAGTTTCCGATCTGGACTTGCCGCAACTGCCCTTTCTCCAGGCGGTGGTGAAGGAAACATTCCGGCTCCACCCACCGACGCCGCTCTCCCTCCCCCGCATGGCCGCCGAGAGCTGCGAAATCAACGGCTACCACATCCCTAAAGGCTCCACTCTCTTGGTGGATGTATGGGCCATTGGTCGTGACCCAAAAGAATGGGCCGACCCACTTGAATTCAGGCCCAATAGGTTCCTCCCAGGCGGAGAGAAGGCCCACGCCGATGTGAAGGGAAATGACTTTGAGGTGATTCCGTTTGGAGCTGGGCGGAGGATCTGCGTGGGTATGAGTATGGGCCTGAGAATGGTCCAGATGTTAACTGCCACGTTGGCCCATTCCTTTGATTGGGCCTTGCCTAATGGGCTTTGGCCAGATGAGTTAAACATGGACGAGGCCTATGGGCTGACTCTGCGTCGGGCCCAACCCTTGGTTGTGCATCCACGGCCCAGATTATTACCTGGGGTTTATCAAAAATTGCCTTAAATTGGTTATTTCTAATTTTCTACTTCTTGGTAGTTAGTTATCTTCTCCAGTTTGTTTTGAAAACATTGTAATGGTTCGATTTTATCAAGACTTTTATTTATTTTATTTTTAATATTGTTGTGTGGGGAGATAAATATCGAG

mRNA sequence

TGTGAATCAATAACCCAAAAATTTATATATATTTAATGGACGAAATTACCCTCATCGTCGCCACCGTAGTTACCTTGGCCTCCGCCATCCTCCTCTACCGTGCTTTCTTCCTCCGCCGCAGATTCCGCCTCCCGCCTGGTCCAAAACCATGGCCGATCGTAGGAAACATGCCGCAAATCGGCGGAGCGCCTCTCCATTCCCTCCTCGCCACCATGGCCGGCGAGTACGGACCTCTGATGTACCTCCGATTAGGCTCCGTTGACATGGTCGTGGCGGCGTCGGCGGCAGTAGCGGAGCAACTCTTGAAGATACACGATACTAATTTCCTTAGCCGCCCGCCGAACGCCGGCGCAAAATACATTGCTTATAATTATCAGGATATGGTTTTTGCGCCGTACGGTCCACGGTGGCGATTACTCCGGAAAATCAGCGCTGTCCATCTCTTCTCCTCAAAAGCCCTAGATGATCATCGCCGCATACGCGAGGAGGAGATAGCGATGCTAGTACAGGCTCTTGCAAGGTCCGGCGAAGCTCCGGCGACCTTGGGGAGCTTGCTCACCGTGTGCACTGCGAATGCGCTCGGACGGACAATGCTCGGCCGACGGGTTTTCGGCGATGGCAGCGGCGGCAACGATCCAGAGGCGAACCGATTTAAGGAAATGGTCGAGCAAGTGATGGTGCTGGCCGGAAAGTTCAACCCCGGCGACTTTTTCCCGTCGCTTGAGTGGCTTGATTTGTTGGGTGTGGCAAGTGAGATGAAGAAGGTCCACAAATGGTTCGACGATTTTTTGACCAAGATCTTGGAGGAGCACCGGAATTTATTGGCCGCAGGCCGTGGCGGCGGCGGCGACCACCACCAAGACTTGTTGAGTACTCTTCTGTCGTCAAAGGATGAAGCTGAGGATGAGAATGAGAAACTTAATGACACTGAGATCAAGGCTTTGTTGTTGAATATGTTTACCGCTGGAACCGACACTTCAGCAAGCACAGTGGAATGGGCCTTGACAGAGCTCATCAGACATCCCAAAATGATGGCCCAGGCCCAACAAGAATTGGACTCCGCAGTGGGCCGTGATCGGGCAGTTTCCGATCTGGACTTGCCGCAACTGCCCTTTCTCCAGGCGGTGGTGAAGGAAACATTCCGGCTCCACCCACCGACGCCGCTCTCCCTCCCCCGCATGGCCGCCGAGAGCTGCGAAATCAACGGCTACCACATCCCTAAAGGCTCCACTCTCTTGGTGGATGTATGGGCCATTGGTCGTGACCCAAAAGAATGGGCCGACCCACTTGAATTCAGGCCCAATAGGTTCCTCCCAGGCGGAGAGAAGGCCCACGCCGATGTGAAGGGAAATGACTTTGAGGTGATTCCGTTTGGAGCTGGGCGGAGGATCTGCGTGGGTATGAGTATGGGCCTGAGAATGGTCCAGATGTTAACTGCCACGTTGGCCCATTCCTTTGATTGGGCCTTGCCTAATGGGCTTTGGCCAGATGAGTTAAACATGGACGAGGCCTATGGGCTGACTCTGCGTCGGGCCCAACCCTTGGTTGTGCATCCACGGCCCAGATTATTACCTGGGGTTTATCAAAAATTGCCTTAAATTGGTTATTTCTAATTTTCTACTTCTTGGTAGTTAGTTATCTTCTCCAGTTTGTTTTGAAAACATTGTAATGGTTCGATTTTATCAAGACTTTTATTTATTTTATTTTTAATATTGTTGTGTGGGGAGATAAATATCGAG

Coding sequence (CDS)

ATGGACGAAATTACCCTCATCGTCGCCACCGTAGTTACCTTGGCCTCCGCCATCCTCCTCTACCGTGCTTTCTTCCTCCGCCGCAGATTCCGCCTCCCGCCTGGTCCAAAACCATGGCCGATCGTAGGAAACATGCCGCAAATCGGCGGAGCGCCTCTCCATTCCCTCCTCGCCACCATGGCCGGCGAGTACGGACCTCTGATGTACCTCCGATTAGGCTCCGTTGACATGGTCGTGGCGGCGTCGGCGGCAGTAGCGGAGCAACTCTTGAAGATACACGATACTAATTTCCTTAGCCGCCCGCCGAACGCCGGCGCAAAATACATTGCTTATAATTATCAGGATATGGTTTTTGCGCCGTACGGTCCACGGTGGCGATTACTCCGGAAAATCAGCGCTGTCCATCTCTTCTCCTCAAAAGCCCTAGATGATCATCGCCGCATACGCGAGGAGGAGATAGCGATGCTAGTACAGGCTCTTGCAAGGTCCGGCGAAGCTCCGGCGACCTTGGGGAGCTTGCTCACCGTGTGCACTGCGAATGCGCTCGGACGGACAATGCTCGGCCGACGGGTTTTCGGCGATGGCAGCGGCGGCAACGATCCAGAGGCGAACCGATTTAAGGAAATGGTCGAGCAAGTGATGGTGCTGGCCGGAAAGTTCAACCCCGGCGACTTTTTCCCGTCGCTTGAGTGGCTTGATTTGTTGGGTGTGGCAAGTGAGATGAAGAAGGTCCACAAATGGTTCGACGATTTTTTGACCAAGATCTTGGAGGAGCACCGGAATTTATTGGCCGCAGGCCGTGGCGGCGGCGGCGACCACCACCAAGACTTGTTGAGTACTCTTCTGTCGTCAAAGGATGAAGCTGAGGATGAGAATGAGAAACTTAATGACACTGAGATCAAGGCTTTGTTGTTGAATATGTTTACCGCTGGAACCGACACTTCAGCAAGCACAGTGGAATGGGCCTTGACAGAGCTCATCAGACATCCCAAAATGATGGCCCAGGCCCAACAAGAATTGGACTCCGCAGTGGGCCGTGATCGGGCAGTTTCCGATCTGGACTTGCCGCAACTGCCCTTTCTCCAGGCGGTGGTGAAGGAAACATTCCGGCTCCACCCACCGACGCCGCTCTCCCTCCCCCGCATGGCCGCCGAGAGCTGCGAAATCAACGGCTACCACATCCCTAAAGGCTCCACTCTCTTGGTGGATGTATGGGCCATTGGTCGTGACCCAAAAGAATGGGCCGACCCACTTGAATTCAGGCCCAATAGGTTCCTCCCAGGCGGAGAGAAGGCCCACGCCGATGTGAAGGGAAATGACTTTGAGGTGATTCCGTTTGGAGCTGGGCGGAGGATCTGCGTGGGTATGAGTATGGGCCTGAGAATGGTCCAGATGTTAACTGCCACGTTGGCCCATTCCTTTGATTGGGCCTTGCCTAATGGGCTTTGGCCAGATGAGTTAAACATGGACGAGGCCTATGGGCTGACTCTGCGTCGGGCCCAACCCTTGGTTGTGCATCCACGGCCCAGATTATTACCTGGGGTTTATCAAAAATTGCCTTAA

Protein sequence

MDEITLIVATVVTLASAILLYRAFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLATMAGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQALARSGEAPATLGSLLTVCTANALGRTMLGRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWLDLLGVASEMKKVHKWFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSKDEAEDENEKLNDTEIKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQLPFLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNGLWPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKLP
Homology
BLAST of CmUC06G123930 vs. NCBI nr
Match: XP_038878698.1 (flavonoid 3'-monooxygenase [Benincasa hispida])

HSP 1 Score: 953.0 bits (2462), Expect = 1.1e-273
Identity = 475/520 (91.35%), Postives = 488/520 (93.85%), Query Frame = 0

Query: 1   MDEITLIVATVVTLASAILLYRAFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLATM 60
           MDEI LIVA VVTLAS ILL+R FF R RFRLPPGPKPWPIVGNMPQIGGAPLHSLLA M
Sbjct: 1   MDEIALIVAAVVTLASTILLHRTFFRRSRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60

Query: 61  AGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AGEYG LMYLRLGSV +VVAASAAVAE+LLK+HDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGEYGSLMYLRLGSVGVVVAASAAVAEKLLKVHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQALARSGEAPATLGSLLTVCTAN 180
           YGPRWRLLRKIS VHLFSSKALDDHRRIREEEIA+LVQALA SGE PA LGSLLTVCTAN
Sbjct: 121 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEIAVLVQALASSGEVPAALGSLLTVCTAN 180

Query: 181 ALGRTMLGRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWLDLLGVASE 240
           ALGRTM+GRRVFGDG  G+D EA +FK MVEQVM+LAGKFNPGDFFPSLEWLDLLGVASE
Sbjct: 181 ALGRTMIGRRVFGDGGSGDDSEAKQFKAMVEQVMILAGKFNPGDFFPSLEWLDLLGVASE 240

Query: 241 MKKVHKWFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSKDEAEDENEKLNDTEI 300
           MKKVHKWFDDFLTKI+EEHRNLLA GRGGG   HQDLLSTLLSS   AEDENEK+NDTEI
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGRGGG---HQDLLSTLLSS---AEDENEKINDTEI 300

Query: 301 KALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQLPF 360
           KALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDS VGRDRAVSDLDLPQL +
Sbjct: 301 KALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSVVGRDRAVSDLDLPQLVY 360

Query: 361 LQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADPLEF 420
           LQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKG+TLLVDVWAIGRDPKEWADPLEF
Sbjct: 361 LQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGTTLLVDVWAIGRDPKEWADPLEF 420

Query: 421 RPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPN 480
           RPNRFLPGGEKAH DVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATL H FDWALPN
Sbjct: 421 RPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLVHYFDWALPN 480

Query: 481 GLWPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKLP 521
           GL PDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQK P
Sbjct: 481 GLLPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKSP 514

BLAST of CmUC06G123930 vs. NCBI nr
Match: XP_008453263.1 (PREDICTED: flavonoid 3'-monooxygenase [Cucumis melo] >KAA0057987.1 flavonoid 3'-monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 939.1 bits (2426), Expect = 1.6e-269
Identity = 463/521 (88.87%), Postives = 489/521 (93.86%), Query Frame = 0

Query: 1   MDEITLIVATVVTLASAILLYRAFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLATM 60
           M+EITLIVA ++TLASA LL+R FFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLA M
Sbjct: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60

Query: 61  AGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AG+YGPLMYLRLGSVDMVVAASAAVAEQ+LKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQALARSGEAPATLGSLLTVCTAN 180
           YGPRWRLLRKISAVHLFSSKALDDHRRIR++EI++LVQALARSGEAPA LGSLLTVC AN
Sbjct: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN 180

Query: 181 ALGRTMLGRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWLDLLGVASE 240
           +LGRTM+GRRVFGDGSG +D E+N+FK MVEQVMVLAGKFNPGDFFP LE LDL+GV SE
Sbjct: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 240

Query: 241 MKKVHKWFDDFLTKILEEHRNLLAAGRGG-GGDHHQDLLSTLLSSKDEAEDENEKLNDTE 300
           MKKVHKWFDDFLTKI+EEHRNLLA G GG GG  HQDLLSTLLS KDE  DENEKLNDTE
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQLP 360
           IKALLLNMFTAGTDTSASTVEWALTELIRHP+MMAQAQQELDS VGRDRAVSDLDLPQL 
Sbjct: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 360

Query: 361 FLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADPLE 420
           +LQAVVKETFRLHPPTPLSLPRMA++SCE+NGYHIPKGSTLLVDVWAIGRDPKEW DPLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 420

Query: 421 FRPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALP 480
           F PNRFLP GEK H DVKGNDFE IPFGAGRRICVG+S+GLRMVQMLTAT+ HSFDWALP
Sbjct: 421 FGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 480

Query: 481 NGLWPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKLP 521
           NGL PD+LNMDE YGLTLRRAQPLVVHPRPRLL GVYQKLP
Sbjct: 481 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521

BLAST of CmUC06G123930 vs. NCBI nr
Match: XP_004138192.1 (flavonoid 3'-monooxygenase [Cucumis sativus] >KGN63725.2 hypothetical protein Csa_014030 [Cucumis sativus])

HSP 1 Score: 922.5 bits (2383), Expect = 1.6e-264
Identity = 453/518 (87.45%), Postives = 484/518 (93.44%), Query Frame = 0

Query: 1   MDEITLIVATVVTLASAILLYRAFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLATM 60
           M+EIT IVA ++TLASA LL+RAFFLRRR RLPPGPKPWPIVGNMPQIG A LHSLLATM
Sbjct: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATM 60

Query: 61  AGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AG+YGPLMYLRLGSVDMVVAASAAVAEQ+LKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQALARSGEAPATLGSLLTVCTAN 180
           YGPRWRLLRKIS VHLFSSKALDDHRRIREEEI++LVQALARSGEAPA LGSLLTVC AN
Sbjct: 121 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIAN 180

Query: 181 ALGRTMLGRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWLDLLGVASE 240
           +LGRTM+GRRVFGDGSG +D E+N+FK MVEQVMVLAGKFNPGDFFP LEWLDL+GV  E
Sbjct: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGRE 240

Query: 241 MKKVHKWFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSKDEAEDENEKLNDTEI 300
           MKKVHKWFDDFLTKI+EEHRNLLA G GGG   HQDLLSTLLS KD+ EDENEKLNDTEI
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGVGGG---HQDLLSTLLSMKDDGEDENEKLNDTEI 300

Query: 301 KALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQLPF 360
           KALLLNMFTAGTDTSASTVEWALTELIRHP+MMAQAQQELDS VGRDRAVSD+DL QL +
Sbjct: 301 KALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVY 360

Query: 361 LQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADPLEF 420
           LQAVVKETFRLHPPTPLSLPRMA++SCE+NGYHIPKGSTLLVDVWAIGRDPK+W DPLEF
Sbjct: 361 LQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEF 420

Query: 421 RPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPN 480
           RPNRFLP GEK H DVKGNDFEVIPFGAGRRICVG+S+GLRMVQMLTAT+ HSFDW LPN
Sbjct: 421 RPNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPN 480

Query: 481 GLWPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQK 519
           GL PD+LNMDE YGLTLRRAQPL++HPRPRLL GVY++
Sbjct: 481 GLTPDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVYER 515

BLAST of CmUC06G123930 vs. NCBI nr
Match: XP_023529494.1 (flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo] >XP_023529495.1 flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 909.4 bits (2349), Expect = 1.4e-260
Identity = 445/520 (85.58%), Postives = 477/520 (91.73%), Query Frame = 0

Query: 1   MDEITLIVATVVTLASAILLYRAFFLRRRFR---LPPGPKPWPIVGNMPQIGGAPLHSLL 60
           MDEI+L  +  +TLAS +LLYR  F +R  R   LPPGPKPWPIVGN+PQIGGAPLHSLL
Sbjct: 1   MDEISLFFSAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLL 60

Query: 61  ATMAGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMV 120
           A M+GEYGPLMYLR GSVD+VVAASAA+AEQ LKI DTNFLSRPPNAGAKYIAYNYQDMV
Sbjct: 61  ADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQALARSGEAPATLGSLLTVC 180
           FAPYGPRWRLLRKISAVHLFSSKAL+DHRR+REEE+ +LVQALARSGEAPATLGSLLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTVC 180

Query: 181 TANALGRTMLGRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWLDLLGV 240
           TANALGRTM+GRRVFGDGSGG DP +++FK MVEQVM+L+GKFNPGDFFPSL+WLDLLGV
Sbjct: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFPSLDWLDLLGV 240

Query: 241 ASEMKKVHKWFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSKDEAEDENEKLND 300
           A EMKKVHKWFDD L+KILEEHRNLLA+G GGGG  HQDLLSTLLS++D AEDENEKLND
Sbjct: 241 AREMKKVHKWFDDLLSKILEEHRNLLASGGGGGGGEHQDLLSTLLSARDRAEDENEKLND 300

Query: 301 TEIKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQ 360
           TEIKALLLNMFTAGTDTSASTVEWAL ELIRHPKMM QAQQELDS VGRDR VSDLDLP+
Sbjct: 301 TEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMHQAQQELDSIVGRDRVVSDLDLPK 360

Query: 361 LPFLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADP 420
           L +LQAVVKETFRLHPPTPLSLPRMAAESCEI+GY IPKGSTLLVDVWAI RDPKEW  P
Sbjct: 361 LGYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYRIPKGSTLLVDVWAISRDPKEWTSP 420

Query: 421 LEFRPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWA 480
           LEFRP+RFLPGGEKAH DVKGNDF VIPFGAGRRICVGMSMGLRMVQ+LTA+L HSFDWA
Sbjct: 421 LEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQILTASLVHSFDWA 480

Query: 481 LPNGLWPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQ 518
           LP+GL P ELNMDEA+GLTLRRAQPLV HPRPRL PGVYQ
Sbjct: 481 LPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 520

BLAST of CmUC06G123930 vs. NCBI nr
Match: XP_022933611.1 (flavonoid 3'-monooxygenase [Cucurbita moschata])

HSP 1 Score: 898.3 bits (2320), Expect = 3.2e-257
Identity = 442/520 (85.00%), Postives = 473/520 (90.96%), Query Frame = 0

Query: 1   MDEITLIVATVVTLASAILLYRAFFLRRRFR---LPPGPKPWPIVGNMPQIGGAPLHSLL 60
           MDEI+L  +  +TLAS +LLYR  F +R  R   LPPGPKPWPIVGN+PQIGGAPLHSLL
Sbjct: 1   MDEISLFFSAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLL 60

Query: 61  ATMAGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMV 120
           A M+GEYGPLMYLR GSVD+VVAASAA+AEQ LKI DTNFLSRPPNAGAKYIAYNYQDMV
Sbjct: 61  ADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQALARSGEAPATLGSLLTVC 180
           FAPYGPRWRLLRKISAVHLFSSKAL+DHRR+REEE+ +LVQALARSGEAPATLGSLLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTVC 180

Query: 181 TANALGRTMLGRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWLDLLGV 240
           TANALGRTM+GRRVFGDGSGG DP +++FK MVEQVM+L+GKFNPGDFF  LEWLDLLGV
Sbjct: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLGV 240

Query: 241 ASEMKKVHKWFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSKDEAEDENEKLND 300
           A EMKKVHKWFDD ++KILEEHRNLLA    GGG  HQDLLSTLLS++D A+DENEKLND
Sbjct: 241 AGEMKKVHKWFDDLMSKILEEHRNLLAT---GGGGEHQDLLSTLLSTRDRAKDENEKLND 300

Query: 301 TEIKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQ 360
           TEIKALLLNMFTAGTDTSASTVEWAL ELIRHPKMM QAQQELDS VGRDR VSDLDLP+
Sbjct: 301 TEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPK 360

Query: 361 LPFLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADP 420
           L +LQAVVKETFRLHPPTPLSLPRMAAESCEI+GYHIPKGSTLLVDVWAI RDPKEW  P
Sbjct: 361 LGYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSP 420

Query: 421 LEFRPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWA 480
           LEFRP+RFLPGGEKAH DVKGNDF VIPFGAGRRICVGMSMGLRMVQMLTA+L HSFDWA
Sbjct: 421 LEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWA 480

Query: 481 LPNGLWPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQ 518
           LP+GL P ELNMDEA+GLTLRRAQPLV HPRPRL PGVYQ
Sbjct: 481 LPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 517

BLAST of CmUC06G123930 vs. ExPASy Swiss-Prot
Match: Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 2.2e-192
Identity = 332/518 (64.09%), Postives = 406/518 (78.38%), Query Frame = 0

Query: 1   MDEITLIVATVVTLASAILLYRAFFLRR-RFRLPPGPKPWPIVGNMPQIGGAPLHSLLAT 60
           M+ ++LI+ TV+       + R+FF +R    LPPGPKPWPI+GN+  +G  P H   A 
Sbjct: 1   MEILSLILYTVIFSFLLQFILRSFFRKRYPLPLPPGPKPWPIIGNLVHLGPKP-HQSTAA 60

Query: 61  MAGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120
           MA  YGPLMYL++G VD+VVAASA+VA Q LK HD NF SRPPN+GA+++AYNYQD+VFA
Sbjct: 61  MAQTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFA 120

Query: 121 PYGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQALARSGEAPATLGSLLTVCTA 180
           PYGPRWR+LRKI +VHLFS+KALDD R +R++E+  L +ALA +G+ P  LG LL VCT 
Sbjct: 121 PYGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNVCTT 180

Query: 181 NALGRTMLGRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWLDLLGVAS 240
           NAL R MLG+RVF DGSG  DP+A  FK MV ++MV+AG FN GDF P L WLD+ GVA+
Sbjct: 181 NALARVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAA 240

Query: 241 EMKKVHKWFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSK-DEAEDENEKLNDT 300
           +MKK+H  FD FLT ILEEH+  +           +DLLSTL+S K D+A+++  KL DT
Sbjct: 241 KMKKLHARFDAFLTDILEEHKGKIFG-------EMKDLLSTLISLKNDDADNDGGKLTDT 300

Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQL 360
           EIKALLLN+F AGTDTS+STVEWA+ ELIR+PK++AQAQQE+D  VGRDR V +LDL QL
Sbjct: 301 EIKALLLNLFVAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQL 360

Query: 361 PFLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADPL 420
            +L+A+VKETFRLHP TPLSLPR+A+ESCEINGY IPKGSTLL++VWAI RDP  WADPL
Sbjct: 361 TYLEAIVKETFRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPL 420

Query: 421 EFRPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWAL 480
           EFRP RFLPGGEK   DV+GNDFEVIPFGAGRRIC GM++G+RMVQ++ ATL H+F+W L
Sbjct: 421 EFRPERFLPGGEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDL 480

Query: 481 PNGLWPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 517
            +G  P+ LNM+EAYGLTL+RA PLVVHPRPRL    Y
Sbjct: 481 VSGQLPEMLNMEEAYGLTLQRADPLVVHPRPRLEAQAY 510

BLAST of CmUC06G123930 vs. ExPASy Swiss-Prot
Match: Q9SD85 (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 1.5e-185
Identity = 319/518 (61.58%), Postives = 397/518 (76.64%), Query Frame = 0

Query: 4   ITLIVATVVTLASAILLYRAFFLRR----RFRLPPGPKPWPIVGNMPQIGGAPLHSLLAT 63
           +T+++ATV+      L+ R F  RR      RLPPGP PWPI+GN+P +G  P H  L+ 
Sbjct: 6   LTILLATVL-----FLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSA 65

Query: 64  MAGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 123
           M   YGP+++LRLG VD+VVAAS +VAEQ LKIHD NF SRPPN+GAK++AYNYQD+VFA
Sbjct: 66  MVTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFA 125

Query: 124 PYGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQALARSGEAPATLGSLLTVCTA 183
           PYG RWRLLRKIS+VHLFS+KAL+D + +R+EE+  L + L R G  P  LG L+ +C  
Sbjct: 126 PYGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVV 185

Query: 184 NALGRTMLGRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWLDLLGVAS 243
           NALGR M+GRR+FG  +   D +A+ F+ MV ++M LAG FN GDF PSL+WLDL GVA 
Sbjct: 186 NALGREMIGRRLFGADA---DHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAG 245

Query: 244 EMKKVHKWFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSK-DEAEDENEKLNDT 303
           +MK++HK FD FL+ IL+EH         G    H D+LSTL+S K  + + +   L DT
Sbjct: 246 KMKRLHKRFDAFLSSILKEHE------MNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDT 305

Query: 304 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQL 363
           EIKALLLNMFTAGTDTSASTV+WA+ ELIRHP +M +AQ+ELD  VGRDR V++ D+ QL
Sbjct: 306 EIKALLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQL 365

Query: 364 PFLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADPL 423
           P+LQAV+KE FRLHPPTPLSLP +A+ESCEINGYHIPKGSTLL ++WAI RDP +W+DPL
Sbjct: 366 PYLQAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPL 425

Query: 424 EFRPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWAL 483
            F+P RFLPGGEK+  DVKG+DFE+IPFGAGRRIC G+S+GLR +Q LTATL   FDW L
Sbjct: 426 AFKPERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWEL 485

Query: 484 PNGLWPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 517
             G+ P++LNM+E+YGLTL+RA PLVVHP+PRL P VY
Sbjct: 486 AGGVTPEKLNMEESYGLTLQRAVPLVVHPKPRLAPNVY 508

BLAST of CmUC06G123930 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 1.2e-169
Identity = 300/518 (57.92%), Postives = 378/518 (72.97%), Query Frame = 0

Query: 4   ITLIVATVVTLASAILLYRAFFLRRRFR---LPPGPKPWPIVGNMPQIGGAPLHSLLATM 63
           +T     + TL  +  +    + RR  +   LPPGP  WPI+GN+PQ+G  P H  L  +
Sbjct: 2   LTFFFLWISTLLLSSFIVYLLYRRRSAQCPPLPPGPNGWPILGNLPQLGAKP-HQTLDAL 61

Query: 64  AGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 123
           + +YGPL  LRLGSV++VVA+S+AVA Q L+ HD NF +RPPN+GA+++AYNYQD+VFAP
Sbjct: 62  SKQYGPLFRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAP 121

Query: 124 YGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQALARSGEAP--ATLGSLLTVCT 183
           YGPRWR+LRK+ +VHLFS KALDD R +R+ E+A LV+ L R  +      LG  L VC 
Sbjct: 122 YGPRWRMLRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCA 181

Query: 184 ANALGRTMLGRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWLDLLGVA 243
            NAL R MLGRRVF D       EA+ FKEMV ++M LAG FN GDF P L WLDL GV 
Sbjct: 182 TNALARAMLGRRVFAD-EDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVV 241

Query: 244 SEMKKVHKWFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSKDEAEDENEKLNDT 303
            +MK++H+ +D FL +++EE++    +G         DLLS L+  K+   +   KLN+T
Sbjct: 242 GKMKRLHRRYDAFLDRVIEENQANAKSG---------DLLSVLIRLKEADAEGEIKLNNT 301

Query: 304 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQL 363
           +IKALLLN+FTAGTDTS+STVEW L ELIRHP ++ + Q ELDS +GRDR V++ DLP L
Sbjct: 302 DIKALLLNLFTAGTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNL 361

Query: 364 PFLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADPL 423
           P+LQAVVKETFRLHP TPLSLPRMA+E C +NGY IPK +TLLV+VW+IGRD   W DPL
Sbjct: 362 PYLQAVVKETFRLHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPL 421

Query: 424 EFRPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWAL 483
           EFRP+RFLPGGE+ H DVKGNDFEVIPFGAGRRIC G+S+GLRMVQ +TAT+ H++DW+L
Sbjct: 422 EFRPSRFLPGGEREHVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSL 481

Query: 484 PNGLWPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 517
           P G    +L+M+EAYGLTL+RA PL+V P PRL    Y
Sbjct: 482 PKGQECQKLDMEEAYGLTLQRAVPLMVQPIPRLSHKAY 508

BLAST of CmUC06G123930 vs. ExPASy Swiss-Prot
Match: Q7G602 (Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP75B3 PE=2 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 1.9e-167
Identity = 296/529 (55.95%), Postives = 376/529 (71.08%), Query Frame = 0

Query: 1   MDEITLIVATVVTLASAILLYRAFFL---------RRRFRLPPGPKPWPIVGNMPQIGGA 60
           MD + L +       SA + Y  +FL         R+R  LPPGP+ WP++GN+PQ+G  
Sbjct: 1   MDVVPLPLLLGSLAVSAAVWYLVYFLRGGSGGDAARKRRPLPPGPRGWPVLGNLPQLGDK 60

Query: 61  PLHSLLATMAGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAY 120
           P H++ A +A +YGPL  LR G  ++VVAASA VA Q L+ HD NF +RPPN+GA+++AY
Sbjct: 61  PHHTMCA-LARQYGPLFRLRFGCAEVVVAASAPVAAQFLRGHDANFSNRPPNSGAEHVAY 120

Query: 121 NYQDMVFAPYGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQALARSGEAPATLG 180
           NYQD+VFAPYG RWR LRK+ A+HLFS+KALDD R +RE E+A++V+ LAR   A   LG
Sbjct: 121 NYQDLVFAPYGARWRALRKLCALHLFSAKALDDLRAVREGEVALMVRNLARQQAASVALG 180

Query: 181 SLLTVCTANALGRTMLGRRVFG-DGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLE 240
               VC  N L R  +G RVF  DG  G    A  FKEMV ++M LAG FN GDF P+L 
Sbjct: 181 QEANVCATNTLARATIGHRVFAVDGGEG----AREFKEMVVELMQLAGVFNVGDFVPALR 240

Query: 241 WLDLLGVASEMKKVHKWFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSKDE--- 300
           WLD  GV ++MK++H+ +D+ +   + E +          G+H  DLLS LL+   E   
Sbjct: 241 WLDPQGVVAKMKRLHRRYDNMMNGFINERKAGAQPDGVAAGEHGNDLLSVLLARMQEEQK 300

Query: 301 AEDENEKLNDTEIKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRD 360
            + + EK+ +T+IKALLLN+FTAGTDT++STVEWAL ELIRHP ++ +AQ ELD+ VGR 
Sbjct: 301 LDGDGEKITETDIKALLLNLFTAGTDTTSSTVEWALAELIRHPDVLKEAQHELDTVVGRG 360

Query: 361 RAVSDLDLPQLPFLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAI 420
           R VS+ DLP+LP+L AV+KETFRLHP TPLSLPR AAE CE++GY IPKG+TLLV+VWAI
Sbjct: 361 RLVSESDLPRLPYLTAVIKETFRLHPSTPLSLPREAAEECEVDGYRIPKGATLLVNVWAI 420

Query: 421 GRDPKEWADPLEFRPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLT 480
            RDP +W DPL+++P+RFLPG   A  DVKG DF +IPFGAGRRIC G+S GLRMV ++T
Sbjct: 421 ARDPTQWPDPLQYQPSRFLPGRMHADVDVKGADFGLIPFGAGRRICAGLSWGLRMVTLMT 480

Query: 481 ATLAHSFDWALPNGLWPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 517
           ATL H FDW L NG  PD+LNM+EAYGLTL+RA PL+V P PRLLP  Y
Sbjct: 481 ATLVHGFDWTLANGATPDKLNMEEAYGLTLQRAVPLMVQPVPRLLPSAY 524

BLAST of CmUC06G123930 vs. ExPASy Swiss-Prot
Match: A0A4D6Q414 (Flavonoid 3',5'-hydroxylase CYP75B138 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B138 PE=1 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 1.2e-161
Identity = 286/528 (54.17%), Postives = 383/528 (72.54%), Query Frame = 0

Query: 3   EITLIVATVVTLASAILLYRAFFLRRR---FRLPPGPKPWPIVGNMPQIGGAPLHSLLAT 62
           +I L ++ +V L  +I  Y  F   +R    +LPPGPK +P++GN+PQ+G  P H  L  
Sbjct: 7   DIILFISAIVFL--SIYYYNLFSNAKRSNGLKLPPGPKGYPVLGNLPQLGAKP-HQALQA 66

Query: 63  MAGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 122
            +  YGPLM LRLGSVD+VVA+S +VA Q LK +D+NF +RPPN+GA+++A+NY D+VFA
Sbjct: 67  FSRVYGPLMRLRLGSVDLVVASSPSVAAQFLK-NDSNFCARPPNSGAEHMAFNYHDLVFA 126

Query: 123 PYGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQAL---ARSGEAPATLGSLLTV 182
           PYGPRWRLLRK+SAVHL   KALDD++ +REEE+A+L + L   +R GE P  +G  + V
Sbjct: 127 PYGPRWRLLRKLSAVHLLGPKALDDNQNVREEELAVLARMLYERSRGGE-PVNVGKEMHV 186

Query: 183 CTANALGRTMLGRRVF---GDGSGGNDPE-----ANRFKEMVEQVMVLAGKFNPGDFFPS 242
           C+ NAL R M+GRRVF     G GG + E     A  FK+MV +VM LAG FN GDF P 
Sbjct: 187 CSTNALSRAMMGRRVFEKLAVGGGGVEEEEEMKKAEEFKDMVVEVMTLAGVFNIGDFVPW 246

Query: 243 LEWLDLLGVASEMKKVHKWFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSKDEA 302
           L+  D+ GV  +MK+VH+ ++ FL K + E R+    G         DLLS L+  + ++
Sbjct: 247 LKPFDIQGVVRKMKRVHRRYNVFLDKFIAECRSSAKPGA-------NDLLSVLIGQRGKS 306

Query: 303 EDENEKLNDTEIKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDR 362
           +    ++ DT IKAL+LN+ TAGTDTS+ST+EWALTELIRHP ++ +AQQE+DSAVGRDR
Sbjct: 307 DGSGGEITDTAIKALVLNLLTAGTDTSSSTIEWALTELIRHPDILKKAQQEIDSAVGRDR 366

Query: 363 AVSDLDLPQLPFLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIG 422
            V++ D+P+LP+LQA+VKE FR+HP TPLSLPRM+ E C+I GYHIPK STL V++WA+G
Sbjct: 367 LVTESDVPKLPYLQAIVKENFRMHPATPLSLPRMSIEECDIGGYHIPKNSTLFVNIWAMG 426

Query: 423 RDPKEWADPLEFRPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTA 482
           RDP  W DP+EFRP+RFLPGG+  H +V+GN FE++PFGAGRRIC G SMG+R+V    A
Sbjct: 427 RDPSIWPDPMEFRPSRFLPGGQGEHLEVRGNHFELMPFGAGRRICAGTSMGIRVVHSTVA 486

Query: 483 TLAHSFDWALPNGLWPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 517
           TL H+FDW LP GL  ++++M+EA+G++L++A PL+ HP PRL P  Y
Sbjct: 487 TLIHAFDWKLPEGLTAEKIDMEEAFGISLQKAIPLMAHPIPRLAPKAY 522

BLAST of CmUC06G123930 vs. ExPASy TrEMBL
Match: A0A5A7UTD5 (Flavonoid 3'-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002910 PE=3 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 7.8e-270
Identity = 463/521 (88.87%), Postives = 489/521 (93.86%), Query Frame = 0

Query: 1   MDEITLIVATVVTLASAILLYRAFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLATM 60
           M+EITLIVA ++TLASA LL+R FFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLA M
Sbjct: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60

Query: 61  AGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AG+YGPLMYLRLGSVDMVVAASAAVAEQ+LKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQALARSGEAPATLGSLLTVCTAN 180
           YGPRWRLLRKISAVHLFSSKALDDHRRIR++EI++LVQALARSGEAPA LGSLLTVC AN
Sbjct: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN 180

Query: 181 ALGRTMLGRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWLDLLGVASE 240
           +LGRTM+GRRVFGDGSG +D E+N+FK MVEQVMVLAGKFNPGDFFP LE LDL+GV SE
Sbjct: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 240

Query: 241 MKKVHKWFDDFLTKILEEHRNLLAAGRGG-GGDHHQDLLSTLLSSKDEAEDENEKLNDTE 300
           MKKVHKWFDDFLTKI+EEHRNLLA G GG GG  HQDLLSTLLS KDE  DENEKLNDTE
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQLP 360
           IKALLLNMFTAGTDTSASTVEWALTELIRHP+MMAQAQQELDS VGRDRAVSDLDLPQL 
Sbjct: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 360

Query: 361 FLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADPLE 420
           +LQAVVKETFRLHPPTPLSLPRMA++SCE+NGYHIPKGSTLLVDVWAIGRDPKEW DPLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 420

Query: 421 FRPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALP 480
           F PNRFLP GEK H DVKGNDFE IPFGAGRRICVG+S+GLRMVQMLTAT+ HSFDWALP
Sbjct: 421 FGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 480

Query: 481 NGLWPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKLP 521
           NGL PD+LNMDE YGLTLRRAQPLVVHPRPRLL GVYQKLP
Sbjct: 481 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521

BLAST of CmUC06G123930 vs. ExPASy TrEMBL
Match: A0A1S3BVU8 (flavonoid 3'-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103494036 PE=3 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 7.8e-270
Identity = 463/521 (88.87%), Postives = 489/521 (93.86%), Query Frame = 0

Query: 1   MDEITLIVATVVTLASAILLYRAFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLATM 60
           M+EITLIVA ++TLASA LL+R FFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLA M
Sbjct: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60

Query: 61  AGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AG+YGPLMYLRLGSVDMVVAASAAVAEQ+LKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQALARSGEAPATLGSLLTVCTAN 180
           YGPRWRLLRKISAVHLFSSKALDDHRRIR++EI++LVQALARSGEAPA LGSLLTVC AN
Sbjct: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN 180

Query: 181 ALGRTMLGRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWLDLLGVASE 240
           +LGRTM+GRRVFGDGSG +D E+N+FK MVEQVMVLAGKFNPGDFFP LE LDL+GV SE
Sbjct: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 240

Query: 241 MKKVHKWFDDFLTKILEEHRNLLAAGRGG-GGDHHQDLLSTLLSSKDEAEDENEKLNDTE 300
           MKKVHKWFDDFLTKI+EEHRNLLA G GG GG  HQDLLSTLLS KDE  DENEKLNDTE
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQLP 360
           IKALLLNMFTAGTDTSASTVEWALTELIRHP+MMAQAQQELDS VGRDRAVSDLDLPQL 
Sbjct: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 360

Query: 361 FLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADPLE 420
           +LQAVVKETFRLHPPTPLSLPRMA++SCE+NGYHIPKGSTLLVDVWAIGRDPKEW DPLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 420

Query: 421 FRPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALP 480
           F PNRFLP GEK H DVKGNDFE IPFGAGRRICVG+S+GLRMVQMLTAT+ HSFDWALP
Sbjct: 421 FGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 480

Query: 481 NGLWPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKLP 521
           NGL PD+LNMDE YGLTLRRAQPLVVHPRPRLL GVYQKLP
Sbjct: 481 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521

BLAST of CmUC06G123930 vs. ExPASy TrEMBL
Match: A0A6J1EZJ7 (flavonoid 3'-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440987 PE=3 SV=1)

HSP 1 Score: 898.3 bits (2320), Expect = 1.5e-257
Identity = 442/520 (85.00%), Postives = 473/520 (90.96%), Query Frame = 0

Query: 1   MDEITLIVATVVTLASAILLYRAFFLRRRFR---LPPGPKPWPIVGNMPQIGGAPLHSLL 60
           MDEI+L  +  +TLAS +LLYR  F +R  R   LPPGPKPWPIVGN+PQIGGAPLHSLL
Sbjct: 1   MDEISLFFSAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLL 60

Query: 61  ATMAGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMV 120
           A M+GEYGPLMYLR GSVD+VVAASAA+AEQ LKI DTNFLSRPPNAGAKYIAYNYQDMV
Sbjct: 61  ADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQALARSGEAPATLGSLLTVC 180
           FAPYGPRWRLLRKISAVHLFSSKAL+DHRR+REEE+ +LVQALARSGEAPATLGSLLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTVC 180

Query: 181 TANALGRTMLGRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWLDLLGV 240
           TANALGRTM+GRRVFGDGSGG DP +++FK MVEQVM+L+GKFNPGDFF  LEWLDLLGV
Sbjct: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLGV 240

Query: 241 ASEMKKVHKWFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSKDEAEDENEKLND 300
           A EMKKVHKWFDD ++KILEEHRNLLA    GGG  HQDLLSTLLS++D A+DENEKLND
Sbjct: 241 AGEMKKVHKWFDDLMSKILEEHRNLLAT---GGGGEHQDLLSTLLSTRDRAKDENEKLND 300

Query: 301 TEIKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQ 360
           TEIKALLLNMFTAGTDTSASTVEWAL ELIRHPKMM QAQQELDS VGRDR VSDLDLP+
Sbjct: 301 TEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPK 360

Query: 361 LPFLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADP 420
           L +LQAVVKETFRLHPPTPLSLPRMAAESCEI+GYHIPKGSTLLVDVWAI RDPKEW  P
Sbjct: 361 LGYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSP 420

Query: 421 LEFRPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWA 480
           LEFRP+RFLPGGEKAH DVKGNDF VIPFGAGRRICVGMSMGLRMVQMLTA+L HSFDWA
Sbjct: 421 LEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWA 480

Query: 481 LPNGLWPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQ 518
           LP+GL P ELNMDEA+GLTLRRAQPLV HPRPRL PGVYQ
Sbjct: 481 LPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 517

BLAST of CmUC06G123930 vs. ExPASy TrEMBL
Match: A0A6J1L0D6 (flavonoid 3'-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499893 PE=3 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 1.8e-253
Identity = 436/520 (83.85%), Postives = 469/520 (90.19%), Query Frame = 0

Query: 1   MDEITLIVATVVTLASAILLYRAFFLRRRFR---LPPGPKPWPIVGNMPQIGGAPLHSLL 60
           MDEI+L  +  +TLA+ +LLYR  F +R  R   LPPGPKPWP VGN+PQIGGAPLHS L
Sbjct: 1   MDEISLFFSAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQL 60

Query: 61  ATMAGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMV 120
           A M+GEYGPLMYLR GS+D+VVAASAA+AEQ LKI DTNFLSRPPNAGAKYIAYNYQDMV
Sbjct: 61  AAMSGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQALARSGEAPATLGSLLTVC 180
           FAPYGPRWRLLRKISAVHLFSSKAL+D+R +REEE+ +LVQALAR GEAPATLGSLLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTVC 180

Query: 181 TANALGRTMLGRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWLDLLGV 240
           TANALGRTM+GRRVFGDGSGG DP +++FK MVEQVM+L+GKFNPG+FFPSLEWLDLLGV
Sbjct: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLGV 240

Query: 241 ASEMKKVHKWFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSKDEAEDENEKLND 300
           A EMKKVHKWFDD L+KILEEHR LLA+     G  HQDLLSTLLS++D AEDENEKLND
Sbjct: 241 AREMKKVHKWFDDLLSKILEEHRTLLAS-----GGEHQDLLSTLLSTRDHAEDENEKLND 300

Query: 301 TEIKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQ 360
           TEIKALLLNMFTAGTDTSASTVEWAL ELIRHPKMM QAQQELDS VGRDR VSDLDLP+
Sbjct: 301 TEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPK 360

Query: 361 LPFLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADP 420
           L +LQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAI RDPKEW  P
Sbjct: 361 LGYLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCP 420

Query: 421 LEFRPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWA 480
           LEFRP+RFLPGGEKAH DVKGNDF VIPFGAGRRICVGMSMGLRMVQMLTA+L HSFDWA
Sbjct: 421 LEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWA 480

Query: 481 LPNGLWPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQ 518
           LP+GL P ELNMDEA+GLTLRRAQPLV HPRPRL PGVYQ
Sbjct: 481 LPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 515

BLAST of CmUC06G123930 vs. ExPASy TrEMBL
Match: A0A0A0LP99 (Flavonoid 3' hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G013190 PE=3 SV=1)

HSP 1 Score: 827.4 bits (2136), Expect = 3.3e-236
Identity = 412/509 (80.94%), Postives = 445/509 (87.43%), Query Frame = 0

Query: 1   MDEITLIVATVVTLASAILLYRAFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLATM 60
           M+EIT IVA ++TLASA LL+RAFFLRRR RLPPGPKPWPIVGNMPQIG A LHSLLATM
Sbjct: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATM 60

Query: 61  AGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AG+YGPLMYLRLGSVDMVVAASAAVAEQ+LKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQALARSGEAPATLGSLLTVCTAN 180
           YGPRWRLLRKIS VHLFSSKALDDHRRIREEEI++LVQALARSGEAPA LGSLLTVC AN
Sbjct: 121 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIAN 180

Query: 181 ALGRTMLGRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWLDLLGVASE 240
           +LGRTM+GRRVFGDGSG +D E+N+FK MVEQVMVLAGKFNPGDFFP LEWLDL+GV  E
Sbjct: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGRE 240

Query: 241 MKKVHKWFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSKDEAEDENEKLNDTEI 300
           MKKVHKWFDDFLTKI+EEHRNLLA G GGG   HQDLLSTLLS KD+ EDENEKLNDTEI
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGVGGG---HQDLLSTLLSMKDDGEDENEKLNDTEI 300

Query: 301 KALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQLPF 360
           KALLL +F                           ++  L+  +GRDRAVSD+DL QL +
Sbjct: 301 KALLLVLFL--------------------------SKFYLNCFIGRDRAVSDVDLHQLVY 360

Query: 361 LQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADPLEF 420
           LQAVVKETFRLHPPTPLSLPRMA++SCE+NGYHIPKGSTLLVDVWAIGRDPK+W DPLEF
Sbjct: 361 LQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEF 420

Query: 421 RPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPN 480
           RPNRFLP GEK H DVKGNDFEVIPFGAGRRICVG+S+GLRMVQMLTAT+ HSFDW LPN
Sbjct: 421 RPNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPN 480

Query: 481 GLWPDELNMDEAYGLTLRRAQPLVVHPRP 510
           GL PD+LNMDE YGLTLRRAQPL++HPRP
Sbjct: 481 GLTPDKLNMDEHYGLTLRRAQPLIMHPRP 480

BLAST of CmUC06G123930 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 650.6 bits (1677), Expect = 1.1e-186
Identity = 319/518 (61.58%), Postives = 397/518 (76.64%), Query Frame = 0

Query: 4   ITLIVATVVTLASAILLYRAFFLRR----RFRLPPGPKPWPIVGNMPQIGGAPLHSLLAT 63
           +T+++ATV+      L+ R F  RR      RLPPGP PWPI+GN+P +G  P H  L+ 
Sbjct: 6   LTILLATVL-----FLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSA 65

Query: 64  MAGEYGPLMYLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 123
           M   YGP+++LRLG VD+VVAAS +VAEQ LKIHD NF SRPPN+GAK++AYNYQD+VFA
Sbjct: 66  MVTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFA 125

Query: 124 PYGPRWRLLRKISAVHLFSSKALDDHRRIREEEIAMLVQALARSGEAPATLGSLLTVCTA 183
           PYG RWRLLRKIS+VHLFS+KAL+D + +R+EE+  L + L R G  P  LG L+ +C  
Sbjct: 126 PYGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVV 185

Query: 184 NALGRTMLGRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWLDLLGVAS 243
           NALGR M+GRR+FG  +   D +A+ F+ MV ++M LAG FN GDF PSL+WLDL GVA 
Sbjct: 186 NALGREMIGRRLFGADA---DHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAG 245

Query: 244 EMKKVHKWFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSK-DEAEDENEKLNDT 303
           +MK++HK FD FL+ IL+EH         G    H D+LSTL+S K  + + +   L DT
Sbjct: 246 KMKRLHKRFDAFLSSILKEHE------MNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDT 305

Query: 304 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQL 363
           EIKALLLNMFTAGTDTSASTV+WA+ ELIRHP +M +AQ+ELD  VGRDR V++ D+ QL
Sbjct: 306 EIKALLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQL 365

Query: 364 PFLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADPL 423
           P+LQAV+KE FRLHPPTPLSLP +A+ESCEINGYHIPKGSTLL ++WAI RDP +W+DPL
Sbjct: 366 PYLQAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPL 425

Query: 424 EFRPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWAL 483
            F+P RFLPGGEK+  DVKG+DFE+IPFGAGRRIC G+S+GLR +Q LTATL   FDW L
Sbjct: 426 AFKPERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWEL 485

Query: 484 PNGLWPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 517
             G+ P++LNM+E+YGLTL+RA PLVVHP+PRL P VY
Sbjct: 486 AGGVTPEKLNMEESYGLTLQRAVPLVVHPKPRLAPNVY 508

BLAST of CmUC06G123930 vs. TAIR 10
Match: AT1G01280.1 (cytochrome P450, family 703, subfamily A, polypeptide 2 )

HSP 1 Score: 338.2 bits (866), Expect = 1.2e-92
Identity = 184/489 (37.63%), Postives = 271/489 (55.42%), Query Frame = 0

Query: 31  RLPPGPKPWPIVGNMPQIGGAPLHSLLATMAGEYGPLMYLRLGSVDMVVAASAAVAEQLL 90
           RLPPGP   PI+GN+ Q+G  P H  LA++  +YGPL+YLRLG+VD +         ++L
Sbjct: 31  RLPPGPPRLPILGNLLQLGPLP-HRDLASLCDKYGPLVYLRLGNVDAITTNDPDTIREIL 90

Query: 91  KIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLRKISAVHLFSSKALDDHRRIRE 150
              D  F SRP    A ++AY   D+  AP GP W+ +R+I   HL ++K L+     R 
Sbjct: 91  LRQDDVFSSRPKTLAAVHLAYGCGDVALAPMGPHWKRMRRICMEHLLTTKRLESFTTQRA 150

Query: 151 EEIAMLVQALARSGEA--PATLGSLLTVCTANALGRTMLGRRVFGDGSGGNDPEANRFKE 210
           EE   L++ + +  E   P  L  +L   + N + R +LG++ FG GS  +  EA  F  
Sbjct: 151 EEARYLIRDVFKRSETGKPINLKEVLGAFSMNNVTRMLLGKQFFGPGSLVSPKEAQEFLH 210

Query: 211 MVEQVMVLAGKFNPGDFFPSLEWLDLLGVASEMKKVHKWFDDFLTKILEEHRNLLAAGRG 270
           +  ++  L G    GD+ P   W+D  G   EM+ V K  D+F TKI++EHR        
Sbjct: 211 ITHKLFWLLGVIYLGDYLPFWRWVDPSGCEKEMRDVEKRVDEFHTKIIDEHRRAKLEDED 270

Query: 271 GGGDHHQDLLSTLLSSKDEAEDENEKLNDTEIKALLLNMFTAGTDTSASTVEWALTELIR 330
             GD   D +  LLS     E+    + D EIKAL+ +M  A TDTSA T EWA+ E I+
Sbjct: 271 KNGD--MDFVDVLLSL--PGENGKAHMEDVEIKALIQDMIAAATDTSAVTNEWAMAEAIK 330

Query: 331 HPKMMAQAQQELDSAVGRDRAVSDLDLPQLPFLQAVVKETFRLHPPTPLSLPRMAAESCE 390
            P++M + Q+ELD+ VG +R V + DL  L +L+ VV+ETFR+HP  P  +P  +  +  
Sbjct: 331 QPRVMRKIQEELDNVVGSNRMVDESDLVHLNYLRCVVRETFRMHPAGPFLIPHESVRATT 390

Query: 391 INGYHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHADVK-GNDFEVIPFG 450
           INGY+IP  + + ++   +GR+ K W D  +FRP R  P       ++  G DF+++PF 
Sbjct: 391 INGYYIPAKTRVFINTHGLGRNTKIWDDVEDFRPERHWPVEGSGRVEISHGPDFKILPFS 450

Query: 451 AGRRICVGMSMGLRMVQMLTATLAHSFDWALPNGLWPDELNMDEAYGLTLRRAQPLVVHP 510
           AG+R C G  +G+ MV M  A L H F+W+ P       ++  E YG+T+ +A+PL    
Sbjct: 451 AGKRKCPGAPLGVTMVLMALARLFHCFEWSSPG-----NIDTVEVYGMTMPKAKPLRAIA 509

Query: 511 RPRLLPGVY 517
           +PRL   +Y
Sbjct: 511 KPRLAAHLY 509

BLAST of CmUC06G123930 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 336.3 bits (861), Expect = 4.5e-92
Identity = 184/510 (36.08%), Postives = 298/510 (58.43%), Query Frame = 0

Query: 14  LASAILLYRAFFLRR----RFRLPPGPKPWPIVGNMPQIGGAPLHSLLATMAGEYGPLMY 73
           L S + +  + FL++    +F LPP P   PI+GN+  + G P H     ++ +YGPL++
Sbjct: 9   LVSLLTIVSSIFLKQNKTSKFNLPPSPSSLPIIGNLHHLAGLP-HRCFHKLSIKYGPLVF 68

Query: 74  LRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLR 133
           LRLGSV +VV +S+  AE +LK +D    SRP   G+  ++Y ++D+ FAPYG  WR +R
Sbjct: 69  LRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYWREVR 128

Query: 134 KISAVHLFSSKALDDHRRIREEEIAMLVQALARSG--EAPATLGSLLTVCTANALGRTML 193
           K++ + LFSSK +   R IREEE+  +V+ ++ S   ++P  L       TA+ + R  L
Sbjct: 129 KLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASIICRVAL 188

Query: 194 GRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPS-----LEWLDLLGVASEMK 253
           G+        G   + +R +E+V +     G F   DFFP      ++W  L     ++ 
Sbjct: 189 GQNF---NESGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRFVDW--LFQRHKKIN 248

Query: 254 KVHKWFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSKDEAEDENE-KLNDTEIK 313
           KV K  D F   ++++H  L   GR      +QD+++ +L   D+ ED +  KLN   +K
Sbjct: 249 KVFKELDAFYQHVIDDH--LKPEGR-----KNQDIVTLILDMIDKQEDSDSFKLNMDNLK 308

Query: 314 ALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVG-RDRAVSDLDLPQLPF 373
           A+++++F AG DTSA T+ WA+TELIR+P++M +AQ+ + + +G +   +++ DL ++ +
Sbjct: 309 AIVMDVFLAGIDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVEY 368

Query: 374 LQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADPLEF 433
           L  ++KETFRLHP  P  +PR      +I GY IP  + + ++VW IGRDPK W DP EF
Sbjct: 369 LNHILKETFRLHPALPFVVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWNDPEEF 428

Query: 434 RPNRFLPGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPN 493
            P RF      +  D +G  F+++PFG+GRRIC GM M +  V++    L + FDW++P+
Sbjct: 429 NPERF----ANSSVDFRGQHFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSMPD 488

Query: 494 GLWPDELNMDEAYGLTLRRAQPLVVHPRPR 511
           G   ++++M+EA  +++ +  PL + P  R
Sbjct: 489 GTKGEDIDMEEAGNISIVKKIPLQLVPVQR 501

BLAST of CmUC06G123930 vs. TAIR 10
Match: AT4G12320.1 (cytochrome P450, family 706, subfamily A, polypeptide 6 )

HSP 1 Score: 335.9 bits (860), Expect = 5.8e-92
Identity = 182/505 (36.04%), Postives = 280/505 (55.45%), Query Frame = 0

Query: 9   ATVVTLASAILLYRAFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLATMAGEYGPLM 68
           A ++ +A   +L+  F    +  LPPGP+  PIVGN+P +    LH+    +A  YGP+ 
Sbjct: 19  AILILIAIFSILWYLFKRSPQPHLPPGPRGLPIVGNLPFL-DPDLHTYFTKLAESYGPIF 78

Query: 69  YLRLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLL 128
            L LGS   VV  + ++A ++LK  D NF +      A+ + Y   D+V+ PYG  WR+L
Sbjct: 79  KLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWRML 138

Query: 129 RKISAVHLFSSKALDDHRRIREEEIAMLVQALARSG--EAPATLGSLLTVCTANALGRTM 188
           RK+  + L S + L+    +R +EI    + L + G  E+P  +G  + +   N     +
Sbjct: 139 RKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLTMMNLTMNML 198

Query: 189 LGRRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWLDLLGVASEMKKVHK 248
            G  V  +     +     FKE++ ++  L G+ N  DFFP L   DL G+  +M    +
Sbjct: 199 WGGSVKAEEM---ESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQGLVKKMHVCAR 258

Query: 249 WFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSKDEAEDENEKLNDTEIKALLLN 308
             D  L + +E+ +  L   R G     +D L  L+  KD+  D    +    +KA+L++
Sbjct: 259 ELDAILDRAIEQMQ--LLRTRDGDDGECKDFLQHLMKLKDQEADSEVPITVNHVKAVLVD 318

Query: 309 MFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQLPFLQAVVK 368
           +   GTDTS +T+E+A+ ELIR P++M +AQQELD  VG+D  + +  + +LPF+ A++K
Sbjct: 319 LVVGGTDTSTNTIEFAMAELIRKPELMKRAQQELDEVVGKDNIIEESHITRLPFISAIMK 378

Query: 369 ETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFL 428
           ET RL+P  PL +P   +E+  + GY IPK + + ++VW+I RDP  W  P EFRP RFL
Sbjct: 379 ETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFRPERFL 438

Query: 429 PGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNGLWPDE 488
              +K   D  G D+  +PFG+GRRIC G+++  RM+    ATL HSFDW +P G     
Sbjct: 439 ---DKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWKIPEG---HI 498

Query: 489 LNMDEAYGLTLRRAQPLVVHPRPRL 512
           L++ E +G+ L+   PLV  P PRL
Sbjct: 499 LDLKEKFGIVLKLKSPLVALPVPRL 511

BLAST of CmUC06G123930 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 335.5 bits (859), Expect = 7.6e-92
Identity = 187/504 (37.10%), Postives = 292/504 (57.94%), Query Frame = 0

Query: 11  VVTLASAILLYRAFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLATMAGEYGPLMYL 70
           +V +  A+  ++     R+F  PPG   +PI+GN+ QIG  P H  L  ++ +YGP+M+L
Sbjct: 12  LVCILLAVFNHKKHPKYRQFPCPPG---FPIIGNLHQIGELP-HQTLWKLSKKYGPVMHL 71

Query: 71  RLGSVDMVVAASAAVAEQLLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLRK 130
            LG V  VV +S+  A Q+L++HD +  +RP  +G + ++YNY D+ F+PY   W+ +RK
Sbjct: 72  MLGRVPTVVVSSSDTARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAFSPYDDYWKEVRK 131

Query: 131 ISAVHLFSSKALDDHRRIREEEIAMLVQALARSG--EAPATLGSLLTVCTANALGRTMLG 190
           +    LFS+K +   + I++EE+  ++ ++A S   + P  L +     T + + RT   
Sbjct: 132 LCVQELFSTKQVHSIQPIKDEEVKKMIDSIAESASQKNPVNLNNKCLELTVSVVCRT--- 191

Query: 191 RRVFGDGSGGNDPEANRFKEMVEQVMVLAGKFNPGDFFPSLEWL--DLLGVASEMKKVHK 250
              FG    G    ++RF ++V + + + G F+  DF P + W+   L G+    ++  +
Sbjct: 192 --AFGVSFEGTVLNSDRFNKIVREALEMLGSFSAADFIPYVGWIIDVLTGLQGRRERSKR 251

Query: 251 WFDDFLTKILEEHRNLLAAGRGGGGDHHQDLLSTLLSSKDEAEDENEKLNDTEIKALLLN 310
             + F  ++ + H+     G+  G +   DLL  L   K+EA   N+KL    IKA+LL+
Sbjct: 252 DLNAFFEQMFDLHKE----GKKEGNEDFVDLLLRL--EKEEAVLGNDKLTRNHIKAILLD 311

Query: 311 MFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSAVGRDRAVSDLDLPQLPFLQAVVK 370
           +  AG DTSA T+ WA+TEL R+P++M + Q E+ + +G    +S  D+ QL +L+ V+K
Sbjct: 312 VLLAGIDTSAITMTWAMTELARNPRVMKKVQSEIRTQMGNRSMISFEDMDQLEYLKMVIK 371

Query: 371 ETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFL 430
           ET+RLHP TPL LPR A    +INGY IP  + L V+VWAIGRDP  W DP  F P RF+
Sbjct: 372 ETWRLHPTTPLLLPREAMSEFDINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFM 431

Query: 431 PGGEKAHADVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNGLWPDE 490
                 + D KG  FE++PFG GRRIC  + MG  MV+   A L + FDW LP G+   +
Sbjct: 432 DN----NIDAKGQHFELLPFGGGRRICPAIYMGTTMVEFGLANLLYHFDWKLPEGVEVKD 491

Query: 491 LNMDEAYGLTLRRAQPLVVHPRPR 511
           ++++EA GLT+ +   L++ P  R
Sbjct: 492 IDVEEAPGLTVNKKNELLLVPEMR 496

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878698.11.1e-27391.35flavonoid 3'-monooxygenase [Benincasa hispida][more]
XP_008453263.11.6e-26988.87PREDICTED: flavonoid 3'-monooxygenase [Cucumis melo] >KAA0057987.1 flavonoid 3'-... [more]
XP_004138192.11.6e-26487.45flavonoid 3'-monooxygenase [Cucumis sativus] >KGN63725.2 hypothetical protein Cs... [more]
XP_023529494.11.4e-26085.58flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo] >XP_023529495.1 flavonoi... [more]
XP_022933611.13.2e-25785.00flavonoid 3'-monooxygenase [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SBQ92.2e-19264.09Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1[more]
Q9SD851.5e-18561.58Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1[more]
A0A4D6Q4151.2e-16957.92Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN... [more]
Q7G6021.9e-16755.95Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
A0A4D6Q4141.2e-16154.17Flavonoid 3',5'-hydroxylase CYP75B138 OS=Crocosmia x crocosmiiflora OX=1053288 G... [more]
Match NameE-valueIdentityDescription
A0A5A7UTD57.8e-27088.87Flavonoid 3'-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S3BVU87.8e-27088.87flavonoid 3'-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103494036 PE=3 SV=1[more]
A0A6J1EZJ71.5e-25785.00flavonoid 3'-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440987 PE=3 SV... [more]
A0A6J1L0D61.8e-25383.85flavonoid 3'-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499893 PE=3 SV=1[more]
A0A0A0LP993.3e-23680.94Flavonoid 3' hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G013190 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G07990.11.1e-18661.58Cytochrome P450 superfamily protein [more]
AT1G01280.11.2e-9237.63cytochrome P450, family 703, subfamily A, polypeptide 2 [more]
AT1G13080.14.5e-9236.08cytochrome P450, family 71, subfamily B, polypeptide 2 [more]
AT4G12320.15.8e-9236.04cytochrome P450, family 706, subfamily A, polypeptide 6 [more]
AT3G26310.17.6e-9237.10cytochrome P450, family 71, subfamily B, polypeptide 35 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 278..301
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 10..508
NoneNo IPR availablePANTHERPTHR24298:SF45FLAVONOID 3'-MONOOXYGENASEcoord: 10..508
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 362..380
score: 40.21
coord: 453..476
score: 21.78
coord: 61..80
score: 33.88
coord: 443..453
score: 57.81
coord: 85..106
score: 21.84
coord: 299..316
score: 32.51
coord: 319..345
score: 31.73
coord: 403..427
score: 38.41
IPR001128Cytochrome P450PRINTSPR00385P450coord: 363..374
score: 45.39
coord: 453..464
score: 23.55
coord: 310..327
score: 38.24
coord: 444..453
score: 60.9
IPR001128Cytochrome P450PFAMPF00067p450coord: 33..481
e-value: 2.6E-105
score: 352.8
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 26..511
e-value: 6.2E-130
score: 436.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 33..510
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 446..455

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC06G123930.1CmUC06G123930.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen