CmUC06G123530 (gene) Watermelon (USVL531) v1

Overview


Name	CmUC06G123530
Type	gene
Organism	Citrullus mucosospermus (Watermelon (USVL531) v1)
Description	protein MIS12 homolog
Location	CmU531Chr06: 27048413 .. 27051484 (+)
RNA-Seq Expression	CmUC06G123530
Synteny	CmUC06G123530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGGAAGTAAAGGCGAAGCAGTATTCGATTCACTGAATCTGAATCCGCAGCTCTTCATTAATGAAGCTCTCAATATTGTCGACGATTTGCTTGATGACGCTTTCGATTTCTATCAATCGTATTGTTCTTTCTGTTTCCATCTCTTCAACATAGGTTTTCAATTTTGTTCGGTTAATTTTATTGATTCTTGTCAATTCCTTTTCCCTTCAGACAAGCATCGGCCGCCTTGAAGACCGAGGGCTCCGACAGATCCCAGGATCTTACCCTGGTAAGGTTTAATTTGTATTATATTACTTGGCATATTCTTATCAGCCGTTAATTTCTGATTTCTTAAGTTTGTGCTCTTAAGCACTTCTTTGAATTGTTCCGATTTTCTTTGAACAAAATTGTTTATTTGCCTCGTTTCTGTAGGGAATATCGCGAGTCCGAGCGTTGGTCCAATTGGGTCTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTCAATCACTGTTTTTCAGTTCCTGAAGGTTTTTCTTTACCCTCAAATGTAGGTTTTTCTTCTACTCTTTCTGTGATAAGGCTCCGGTGCCCAAGGATAAAAATATCGAAGTCCGTATCAGTATCGAAATTTCAAGTAATAGTTTATCAATTGAATATCAATATCGACAGATATTTTTGTGTAAATCAATAAGTTTATCCAAAAAAAAAAAATTAAATTAATAATTAAGTTATTTTTGCTTTCAAAGAAGTTGTAGTTCTTATTGAGATTAATAGATGACAGCTTCTTATGTTTTTTATATATTTATAAAAGATACCGAGATGTCTAAGAACCCTTTATACCAATGTCAAATCTTCCTATTTATAGATATATCAACATATCAACAAATAATTGAAACCTTGTCTGTGCTTTGTTACGTGAAGTTGCTCTATTAGTGTTGTTTTTATGACTGAATTAGTGGCCCTCTATGTCCGAATCATTTCACAAGCGATTAATAATTGAGTATGTAAAATGGCTGCTTGTTGATTTTGCGAGTTTCCCACTTCTTGTGTACTGATTAGAGAGCTTCCTCAAATTTTGGTTCTGGGGCGAATAAATTTTTGGAACTTTGGAGAAATGTGCATCATCAACTTTCAAGTTTTTTACAAGATATACTCATTGTTTAGATGCAGTTTCGAGTTTCCATATTTTCTGACCTTAGAGCTTGCACATTTTGTTTGCTATAAGTCAATGTGGTTCCACTGTCACTAGAACTATGGTCATGATGGTAAAAGATTGTTTTTAGCTCTAAAAGGGAATGTAAGCAAATGTCTGCAGAATTTTTTAGAAAATCTGTCGTTGGTGCATCATGGCTTTTCTTTTTCAATCATTTTTTAAAGTCTAAACTATGTGAATTCCAGTTCGACTCCTTTCATTTTGAACCAATATAGTCACTTGGGGTTGGGCAATCTCTGTCTGTTTCACTGGCATCTCAATCGAATCTGCGATATCTTATTGTCAAGTTGCTAAATTATCAATGGTTCCCATATTCTGTTTGTGAACTTTGAGTTATGGTTAATTGAGTCTATGTATTCTCTTACTACTTTTTCTTTTTTCTAATGCAATAAACATATTTCTGATTCAGATCTAAATATTTTCTTTAAGTTGTCTTAGGATGAATCACCTGGTGTCACTTCAATAAGTCATGACTATGACGTGGATCTGGATACAGAGTTAGATTTTTTGAGGAATAAGCTCTCTGAGGTATTGAGTGTGTCGTATGTTTATTACTTTTGTGTTTATGTATATCATATTTCATGAGGAAATGTATTTTTCAGGCCAGAAAAGAGAATATTGTATTGAGCCAAGAGCTACAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATTAATCATTTCAACGAAGCATTACAACTATATGAGCAAAATTCTGTGAATGAGATGTTCCAGGGTAATTAAATTGTGCTTAATTAAAATCATTTTTCTCTTCCTGCACTCCTGGGTTTACTTGCGCCCTACTCTACTAGTATACTCATGAAGGCATACTCGAAATGAAATATACAAATAATGTATGAAGCTGTTTTTTCTTATAATGAACCTTTATTGTCATAGTCAGCTTGGTAGAGCAAAGTTTCTGTAAACACTAACCTTCTCTCTGACGGATCAATCTGGCCTGCATATCTCATGGTGACACAAATTCTAATAGTTCCAAATGATACTTTTTATTGAACACCTGCAAAATCACTTCATTTAGGCAATTTTTAGATTACCCTAAAGTAAATTCTGGTAGTCCCATTTTCTGTTGTCCATTCCATAAAAGAGGAACTTCAGTATGTGAATAAGGCAATTTATTTTCCTATTTTCATTTTGAAGGCCACTTGGACAAAATTTATTCACTAGTATTAAATTTCATTGTACTGAAACTTTAACGGTTGGCTGGTCATTTGGGGTTTGTGCTATTTTGTGGGATATTTGGGAGAAGACAAATAACAAAGTGTTTAGAGCTGTGGCTGAACCATGGTGATGTTTGGTCCTTGGTGAGGTTTCATGTTTCTCTTTAGAATTCAGTTTCAAAAACCTTTTGTAACCATCCTCTAGATAGTGTCTTATTTAGTTGGAAACCCTTCCTATAGAGGGGTTTTGCAGGCTTGTCCTTTTTGTTGTCTTGTATTCTTCATTTTTTATCTATAAAAACCGTTTTCCCTATCAAACTTCAATGGTTGGCAAGTTAAAGCTGTTCATGATTTTAGGAAGCAGACTTCTTATGCATATCAACTTCTTGCAGAAATGATGGGAACTGCATCGGAGCTACGAGCGAAAATAGGAAAGCTGAAGAAAAGGGGGATGGAGGAAGCCAAGCTCAGTAAAGTAGAGAAGGTTCATACAAACGGAGACATCTCTCACCATCACAATGGTAGTAGGATTTTCAAGCTACTTTATTATCAATCAAGCTCATTTTAGCTTTCTGGAAGTTGAATTTCATCTCACATGCTGGTTGATACTTGTTTCTGTTCTGTAGGTTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTCTTAGCTGATTTGAAGACCCTTTGA

mRNA sequence

ATGGAAGGAAGTAAAGGCGAAGCAGTATTCGATTCACTGAATCTGAATCCGCAGCTCTTCATTAATGAAGCTCTCAATATTGTCGACGATTTGCTTGATGACGCTTTCGATTTCTATCAATCACAAGCATCGGCCGCCTTGAAGACCGAGGGCTCCGACAGATCCCAGGATCTTACCCTGGGAATATCGCGAGTCCGAGCGTTGGTCCAATTGGGTCTGGATAAGCGGTTGGCCATGTGGGAGAAGTTTTTCTTCTACTCTTTCTGTGATAAGGCTCCGGTGCCCAAGGATAAAAATATCGAAGATGAATCACCTGGTGTCACTTCAATAAGTCATGACTATGACGTGGATCTGGATACAGAGTTAGATTTTTTGAGGAATAAGCTCTCTGAGGCCAGAAAAGAGAATATTGTATTGAGCCAAGAGCTACAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATTAATCATTTCAACGAAGCATTACAACTATATGAGCAAAATTCTGTGAATGAGATGTTCCAGGAAATGATGGGAACTGCATCGGAGCTACGAGCGAAAATAGGAAAGCTGAAGAAAAGGGGGATGGAGGAAGCCAAGCTCAGTAAAGTAGAGAAGGTTCATACAAACGGAGACATCTCTCACCATCACAATGGTTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTCTTAGCTGATTTGAAGACCCTTTGA

Coding sequence (CDS)

ATGGAAGGAAGTAAAGGCGAAGCAGTATTCGATTCACTGAATCTGAATCCGCAGCTCTTCATTAATGAAGCTCTCAATATTGTCGACGATTTGCTTGATGACGCTTTCGATTTCTATCAATCACAAGCATCGGCCGCCTTGAAGACCGAGGGCTCCGACAGATCCCAGGATCTTACCCTGGGAATATCGCGAGTCCGAGCGTTGGTCCAATTGGGTCTGGATAAGCGGTTGGCCATGTGGGAGAAGTTTTTCTTCTACTCTTTCTGTGATAAGGCTCCGGTGCCCAAGGATAAAAATATCGAAGATGAATCACCTGGTGTCACTTCAATAAGTCATGACTATGACGTGGATCTGGATACAGAGTTAGATTTTTTGAGGAATAAGCTCTCTGAGGCCAGAAAAGAGAATATTGTATTGAGCCAAGAGCTACAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATTAATCATTTCAACGAAGCATTACAACTATATGAGCAAAATTCTGTGAATGAGATGTTCCAGGAAATGATGGGAACTGCATCGGAGCTACGAGCGAAAATAGGAAAGCTGAAGAAAAGGGGGATGGAGGAAGCCAAGCTCAGTAAAGTAGAGAAGGTTCATACAAACGGAGACATCTCTCACCATCACAATGGTTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTCTTAGCTGATTTGAAGACCCTTTGA

Protein sequence

MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRALVQLGLDKRLAMWEKFFFYSFCDKAPVPKDKNIEDESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRGMEEAKLSKVEKVHTNGDISHHHNGFSNAKLDDIQEFLADLKTL

Homology

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BLAST of CmUC06G123530 vs. NCBI nr
Match: XP_038879945.1 (protein MIS12 homolog [Benincasa hispida])

HSP 1 Score: 389.0 bits (998), Expect = 2.9e-104
Identity = 207/240 (86.25%), Postives = 217/240 (90.42%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGEAVFDSLNLNPQLFINEALNIVDDL+DDAF FYQSQASA LKTEGSDRSQDLTL
Sbjct: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTL 90

Query: 61  GISRVRALVQLGLDKRLAMWEKFFFYSFCDKAPVPKDKNIEDESPGVTSISHDYDVDLDT 120
           GISRVRALVQLGLDKRLAMWEK+   + C   P        D+SPGVTSISHDYDV LDT
Sbjct: 91  GISRVRALVQLGLDKRLAMWEKYCL-NHCFSVPEGFSLPSNDDSPGVTSISHDYDVGLDT 150

Query: 121 ELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHFNEALQLYEQNSVNEMFQEMM 180
           ELDFLRNKLSE RKENIVL+QELQALERQTA+SNSQINHFNEALQLYEQNSVNEMFQEMM
Sbjct: 151 ELDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQEMM 210

Query: 181 GTASELRAKIGKLKKRGMEEAKLSKVEKVHTNGDISHHHNGFSNAKLDDIQEFLADLKTL 240
           GTASELRAKIGKLKKR MEE+KL+KVEKVHTNGDISH+HNG SNAKLDDI+ FL DLKTL
Sbjct: 211 GTASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDLKTL 269

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BLAST of CmUC06G123530 vs. NCBI nr
Match: KAA0058004.1 (Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 380.9 bits (977), Expect = 7.9e-102
Identity = 201/240 (83.75%), Postives = 217/240 (90.42%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VF+SLNLNPQLFINEALN VDDL+DDAFDFYQSQASAALKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKFFFYSFCDKAPVPKDKNIEDESPGVTSISHDYDVDLDT 120
           GIS+VRALVQLGLDKRLAMWEK+   + C   P        DESPGVTSI+HD DVDLDT
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCL-NHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDT 120

Query: 121 ELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHFNEALQLYEQNSVNEMFQEMM 180
           ELD LRNKLSE RKENIVL+QELQALERQTASSNSQI+HFNEALQLYEQ+SVN+MFQEM+
Sbjct: 121 ELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI 180

Query: 181 GTASELRAKIGKLKKRGMEEAKLSKVEKVHTNGDISHHHNGFSNAKLDDIQEFLADLKTL 240
            TASELRAKIGK+KKR  EEAKL+KVEKVHTNGDISHHHNGFSNAKLDDIQEFL+DLK +
Sbjct: 181 KTASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of CmUC06G123530 vs. NCBI nr
Match: XP_023515499.1 (protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 380.2 bits (975), Expect = 1.3e-101
Identity = 202/242 (83.47%), Postives = 215/242 (88.84%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VFDSLNLNPQLFINEALNIVDDL+DDAFDFYQ+QASA+LKTEGSDRSQDL+L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLSL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKFFFYSFCDKAPVPKDKNIEDESPGVTSISHD--YDVDL 120
           GISRVR  VQ GLDKRLAMWEK+   + C   P        DESP VTS+S D  YDVDL
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCL-NHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDL 120

Query: 121 DTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHFNEALQLYEQNSVNEMFQE 180
           DTELDFLRNKLSE RKEN+VL+QELQALERQTASSNSQ+N FNEALQLYEQNSVNEMFQE
Sbjct: 121 DTELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQE 180

Query: 181 MMGTASELRAKIGKLKKRGMEEAKLSKVEKVHTNGDISHHHNGFSNAKLDDIQEFLADLK 240
           MMGTASELRAKIGKLKKR MEE+KL+KVEK+HTNGDISHHH GFSNAKLDDIQEFLADLK
Sbjct: 181 MMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADLK 240

[Close]

BLAST of CmUC06G123530 vs. NCBI nr
Match: KAA0058032.1 (Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 378.3 bits (970), Expect = 5.1e-101
Identity = 199/240 (82.92%), Postives = 216/240 (90.00%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VF+SLNLNPQLFINEALN VDDL+DDAFDFYQSQASA LKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKFFFYSFCDKAPVPKDKNIEDESPGVTSISHDYDVDLDT 120
           GIS+VRALVQLGLDKRLAMWEK+   + C   P        DESPGVTSI+HD DVDLDT
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCL-NHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDT 120

Query: 121 ELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHFNEALQLYEQNSVNEMFQEMM 180
           ELD LRNKLSE RKENIVL+QELQALERQTASSNSQI+HFNEALQLYEQ+SVN+MFQEM+
Sbjct: 121 ELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI 180

Query: 181 GTASELRAKIGKLKKRGMEEAKLSKVEKVHTNGDISHHHNGFSNAKLDDIQEFLADLKTL 240
            TASELR+KIGK+KKR  EEAKL+KVEKVHTNGDISHHHNGFSNAKLDDIQEFL+DLK +
Sbjct: 181 KTASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of CmUC06G123530 vs. NCBI nr
Match: KAG6589575.1 (Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 377.5 bits (968), Expect = 8.7e-101
Identity = 201/242 (83.06%), Postives = 213/242 (88.02%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VFDSLNLNPQLFINEALNIVDDL+DDAFDFYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKFFFYSFCDKAPVPKDKNIEDESPGVTSISHD--YDVDL 120
           GISRVR  VQ GLDKRLAMWEK+   + C   P        DESP VTS+S D  YDVDL
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCL-NHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDL 120

Query: 121 DTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHFNEALQLYEQNSVNEMFQE 180
           DTELDFLRNKLSE RKEN+VL+QELQALERQTASSNSQ+N FNEALQLYEQNSVNEMFQE
Sbjct: 121 DTELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQE 180

Query: 181 MMGTASELRAKIGKLKKRGMEEAKLSKVEKVHTNGDISHHHNGFSNAKLDDIQEFLADLK 240
           MMGTASELRAKIGKLKKR MEE+KL+KVEK+HTNGDISHHH GFSNAKLDDIQEFLADLK
Sbjct: 181 MMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADLK 240

[Close]

BLAST of CmUC06G123530 vs. ExPASy Swiss-Prot
Match: Q2V0Z5 (Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 2.4e-45
Identity = 111/248 (44.76%), Postives = 163/248 (65.73%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSD--RSQDL 60
           MEGSK EAVFDS+NLNPQ+FINEA+N V+D +D AFDFY   AS +LK +GSD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  TLGISRVRALVQLGLDKRLAMWEKFFFYSFCDKAPVPKDKNIEDESPGVTSISHD--YDV 120
           + GI+RVR L+   +D RL +WE +    FC   P   D  +  +S   +S+  D  YD+
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSL-RFCFAVP---DGFVLPKSEESSSVHQDGLYDL 120

Query: 121 DLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHFNEALQLYEQNSVNEMF 180
           +LD ELD LR+KL+   K ++ L  ELQALER + S    +   NEAL+LY+++S++E+F
Sbjct: 121 ELDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIF 180

Query: 181 QEMMGTASELRAKIGKLKKRGMEEAKLSKVEKVHTNGDISHHHNGFS----NAKLDDIQE 240
           +EM   ASELRA + +LK R M+ ++ +KV++      + +H   FS    + KL+D+++
Sbjct: 181 KEMTKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDLEK 238

[Close]

BLAST of CmUC06G123530 vs. ExPASy TrEMBL
Match: A0A5A7US57 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00040 PE=3 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 3.8e-102
Identity = 201/240 (83.75%), Postives = 217/240 (90.42%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VF+SLNLNPQLFINEALN VDDL+DDAFDFYQSQASAALKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKFFFYSFCDKAPVPKDKNIEDESPGVTSISHDYDVDLDT 120
           GIS+VRALVQLGLDKRLAMWEK+   + C   P        DESPGVTSI+HD DVDLDT
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCL-NHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDT 120

Query: 121 ELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHFNEALQLYEQNSVNEMFQEMM 180
           ELD LRNKLSE RKENIVL+QELQALERQTASSNSQI+HFNEALQLYEQ+SVN+MFQEM+
Sbjct: 121 ELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI 180

Query: 181 GTASELRAKIGKLKKRGMEEAKLSKVEKVHTNGDISHHHNGFSNAKLDDIQEFLADLKTL 240
            TASELRAKIGK+KKR  EEAKL+KVEKVHTNGDISHHHNGFSNAKLDDIQEFL+DLK +
Sbjct: 181 KTASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of CmUC06G123530 vs. ExPASy TrEMBL
Match: A0A5A7UQ95 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003370 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 2.5e-101
Identity = 199/240 (82.92%), Postives = 216/240 (90.00%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VF+SLNLNPQLFINEALN VDDL+DDAFDFYQSQASA LKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKFFFYSFCDKAPVPKDKNIEDESPGVTSISHDYDVDLDT 120
           GIS+VRALVQLGLDKRLAMWEK+   + C   P        DESPGVTSI+HD DVDLDT
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCL-NHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDT 120

Query: 121 ELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHFNEALQLYEQNSVNEMFQEMM 180
           ELD LRNKLSE RKENIVL+QELQALERQTASSNSQI+HFNEALQLYEQ+SVN+MFQEM+
Sbjct: 121 ELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI 180

Query: 181 GTASELRAKIGKLKKRGMEEAKLSKVEKVHTNGDISHHHNGFSNAKLDDIQEFLADLKTL 240
            TASELR+KIGK+KKR  EEAKL+KVEKVHTNGDISHHHNGFSNAKLDDIQEFL+DLK +
Sbjct: 181 KTASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of CmUC06G123530 vs. ExPASy TrEMBL
Match: A0A6J1JME3 (protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.4e-99
Identity = 200/242 (82.64%), Postives = 211/242 (87.19%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VFDSLNLNPQLFINEALNIVDDL+DDAFDFYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKFFFYSFCDKAPVPKDKNIEDESPGVTSISHD--YDVDL 120
           GISRVR  VQ GLDKRLAMWEK+   + C   P        DESP VTS+S D  YDVDL
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCL-NHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDL 120

Query: 121 DTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHFNEALQLYEQNSVNEMFQE 180
           DTELDFLR  LSE RKENIVL+QELQALERQTASSNSQ+N FNEALQLYEQNSVNEMFQE
Sbjct: 121 DTELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQE 180

Query: 181 MMGTASELRAKIGKLKKRGMEEAKLSKVEKVHTNGDISHHHNGFSNAKLDDIQEFLADLK 240
           MMGTASELRAKIGKLKKR MEE+KL+KVEK+HTN DISHHH GFSNAKLDDIQEFLADLK
Sbjct: 181 MMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEFLADLK 240

[Close]

BLAST of CmUC06G123530 vs. ExPASy TrEMBL
Match: A0A6J1E0A1 (protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.4e-99
Identity = 199/242 (82.23%), Postives = 211/242 (87.19%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VFDSLNLNPQLFINEALNIVDDL+DDAFDFYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKFFFYSFCDKAPVPKDKNIEDESPGVTSISHD--YDVDL 120
           GISRVR  VQ GLDKRLAMWEK+   + C   P        DESP VTS+S D  YDVDL
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCL-NHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDL 120

Query: 121 DTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHFNEALQLYEQNSVNEMFQE 180
           DTELDFLRNKLSE RKENIVL+QELQALERQ ASSNSQ+N FNEALQLYE+NSVNEMFQE
Sbjct: 121 DTELDFLRNKLSEVRKENIVLNQELQALERQAASSNSQVNRFNEALQLYERNSVNEMFQE 180

Query: 181 MMGTASELRAKIGKLKKRGMEEAKLSKVEKVHTNGDISHHHNGFSNAKLDDIQEFLADLK 240
           MMGTASELRAKIGKLKKR MEE+KL+KVEK+HTNGD SHHH GFSNAKLDDIQEFLADLK
Sbjct: 181 MMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDASHHHKGFSNAKLDDIQEFLADLK 240

[Close]

BLAST of CmUC06G123530 vs. ExPASy TrEMBL
Match: A0A6J1JD43 (protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 3.3e-98
Identity = 200/243 (82.30%), Postives = 211/243 (86.83%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VFDSLNLNPQLFINEALNIVDDL+DDAFDFYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKFFFYSFCDKAPVPKDKNIEDESPGVTSISHD--YDVDL 120
           GISRVR  VQ GLDKRLAMWEK+   + C   P        DESP VTS+S D  YDVDL
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCL-NHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDL 120

Query: 121 DTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHFNEALQLYEQNSVNEMFQ- 180
           DTELDFLR  LSE RKENIVL+QELQALERQTASSNSQ+N FNEALQLYEQNSVNEMFQ 
Sbjct: 121 DTELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQA 180

Query: 181 EMMGTASELRAKIGKLKKRGMEEAKLSKVEKVHTNGDISHHHNGFSNAKLDDIQEFLADL 240
           EMMGTASELRAKIGKLKKR MEE+KL+KVEK+HTN DISHHH GFSNAKLDDIQEFLADL
Sbjct: 181 EMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEFLADL 240

[Close]

BLAST of CmUC06G123530 vs. TAIR 10
Match: AT5G35520.1 (minichromosome instability 12 (mis12)-like )

HSP 1 Score: 183.7 bits (465), Expect = 1.7e-46
Identity = 111/248 (44.76%), Postives = 163/248 (65.73%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSD--RSQDL 60
           MEGSK EAVFDS+NLNPQ+FINEA+N V+D +D AFDFY   AS +LK +GSD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  TLGISRVRALVQLGLDKRLAMWEKFFFYSFCDKAPVPKDKNIEDESPGVTSISHD--YDV 120
           + GI+RVR L+   +D RL +WE +    FC   P   D  +  +S   +S+  D  YD+
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSL-RFCFAVP---DGFVLPKSEESSSVHQDGLYDL 120

Query: 121 DLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHFNEALQLYEQNSVNEMF 180
           +LD ELD LR+KL+   K ++ L  ELQALER + S    +   NEAL+LY+++S++E+F
Sbjct: 121 ELDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIF 180

Query: 181 QEMMGTASELRAKIGKLKKRGMEEAKLSKVEKVHTNGDISHHHNGFS----NAKLDDIQE 240
           +EM   ASELRA + +LK R M+ ++ +KV++      + +H   FS    + KL+D+++
Sbjct: 181 KEMTKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDLEK 238

The following BLAST results are available for this feature:

BLAST of CmUC06G123530 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879945.1	2.9e-104	86.25	protein MIS12 homolog [Benincasa hispida]	[more]
KAA0058004.1	7.9e-102	83.75	Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere prote...	[more]
XP_023515499.1	1.3e-101	83.47	protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]	[more]
KAA0058032.1	5.1e-101	82.92	Centromere protein Mis12 [Cucumis melo var. makuwa]	[more]
KAG6589575.1	8.7e-101	83.06	Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CmUC06G123530 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2V0Z5	2.4e-45	44.76	Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1	[more]

BLAST of CmUC06G123530 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7US57	3.8e-102	83.75	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A5A7UQ95	2.5e-101	82.92	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A6J1JME3	1.4e-99	82.64	protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]
A0A6J1E0A1	1.4e-99	82.23	protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1	[more]
A0A6J1JD43	3.3e-98	82.30	protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]

BLAST of CmUC06G123530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35520.1	1.7e-46	44.76	minichromosome instability 12 (mis12)-like	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 115..149
IPR008685	Centromere protein Mis12	PFAM	PF05859	Mis12	coord: 11..140 e-value: 6.7E-11 score: 42.4
IPR008685	Centromere protein Mis12	PANTHER	PTHR14527	PROTEIN MIS12 HOMOLOG	coord: 1..239

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmUC06G123530.1	CmUC06G123530.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0051382	kinetochore assembly
biological_process	GO:0000070	mitotic sister chromatid segregation
biological_process	GO:0034501	protein localization to kinetochore
biological_process	GO:0000278	mitotic cell cycle
cellular_component	GO:0000818	nuclear MIS12/MIND complex
cellular_component	GO:0000775	chromosome, centromeric region
cellular_component	GO:0005634	nucleus