CmUC03G052590 (gene) Watermelon (USVL531) v1

Overview
NameCmUC03G052590
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
DescriptionHigh-affinity nitrate transporter 2.2
LocationCmU531Chr03: 2846301 .. 2848637 (-)
RNA-Seq ExpressionCmUC03G052590
SyntenyCmUC03G052590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCCATACTTTTAATTAACCGTTTCTCCCGAATCCCACTCCAATATAAATACCACAACTCATATCAAACAAATTACAAGATCATTACCTACTAATTGTTTCATTTTGTTGATTGAAAAGTAAAGAGAAAGAAGGAAGGAAAATGGGAGATGTAGAAGGTTCCCCAGGAAGCTCTATGCATGGGGTGACCGGAAGAGAGCAAAGTTTTGCATTTTCCGTAGCTTCCCCAATTGTCCCAACGGACACAACCGCGAAATTTGCATTACCCGTTGACTCTGAGCACAAAGCGAAGGTTTTCAAGGTGTGGTCTTTAGCCAACCCCCACATGAGAACCTTCCACCTTTCTTGGATTTCCTTCTTTACATGCTTCGTCTCCACATTCGCAGCCGCCCCTCTGGTCCCCATCATTCGAGACAATCTCAACCTCACCAAAGTTGACATCGGTAACGCCGGAGTCACCTCCGTGTCTGGCAGTATCTTCTCTCGTCTTGTAATGGGGGCTGTGTGTGACCTTCTGGGGCCACGCTATGGGTGTGCCTTTCTCATTATGCTCTCCGCCCCCACCGTGTTTTGTATGTCCTTTGTGTCCAACGCTGCTGGGTATATAGCTGTGAGATTCATGATTGGCTTCTCGCTTGCCACCTTCGTCTCCTGCCAGTATTGGATGAGCACCATGTTCAACAGTCAGATCATTGGGCTTGTGAATGGGACTGCTGCTGGGTGGGGGAATATGGGCGGAGGAGCCACTCAGCTCATCATGCCTTTGTTGTACGATGTCATTCAGAGAGCTGGCGCTACTCCTTTCACTGCTTGGAGGATTGCCTTCTTTATCCCTGGTTCCCTTCATATTCTCATGGGCATTTTGGTCTTAACTCTTGGCCAGGACTTGCCTGATGGTAACCTTACCAGTCTTCAGAAGAAGGGCAATGTTGCCAAAGATAAATTCTCCAATGTAATTACTTCCTTCCTTCTCCATCTCAATTCATTCTTTAATTTACTTTAATTATAATATAATATCTCCTCACAGATCCTGTGGTACGCTGTCACCAATTACCGAACTTGGATCTTCGTCCTCCTCTATGGCTACTCCATGGGCGTCGAACTTTCCACCGATAACGTCATTGCCGAATACTTCTACGACAGGTAAAATAATTAAATCCACTGCATTGTTGTGATTTTTTAACCTAATATCAAACCCCGTCAGTCAATCCTAATCCTATGTATATGTGTTATGAAATCAACAGTCATAGAAAGTGATCACAATTCGTAAACAATATTATAGAAAAACCACTTATCGTGTGTTACCTAAGTCCACGTACAAATTGAGACACCATTTACATTAACACAGAAATATCGTGGTTTTAGAGTCTCTACTAAGGACGCGTTTTTGACGTGTCCTATAATATATTCAAAGCATTCCGACCTTACGTTCTGAACTTGTGTTTGTCATATTTAACAGGTTTGATCTAAAGCTACACACAGCAGGAATTATCGCAGCAACGTTCGGGATGGCGAATCTAGTGGCTCGACCGTTCGGGGGGTATGCATCGGACGTGGCAGCGCGGTACTTCGGGATGAGAGGAAGGCTGTGGACTTTGTGGATCCTACAAACATGTGGGGGAGTGTTTTGCATGTGGCTTGGGAAAGCAAACAAACTGCCAACAGCAATAGTTGCAATGATATTGTTCTCAATAGGAGCACAGGCTGCATGTGGAGCAACATTTGGGATCATTCCCTTCATTTCTCGACGGTCTTTAGGAATTATATCAGGACTGACAGGAGCAGGAGGAAACTTTGGAAGTGGTTTGACACAATTAGTGTTCTTCTCAACCACCAAATACTCAACAGACACAGGCTTGTTCTTGATGGGAATAATGATTGTATGTTGCACTTTACCAGTCAGTTTGGTTCATTTCCCTCAATGGGGAAGCATGTTTTTACCTCCCACGAAACATGTTGAAAAATCCACTGAGGAATTTTATTATGGTTCTGAATGGACTGAGGATGAAAAGAAGAAGGGTTTGCATCAACAAAGCATTAAGTTTGCTGAGAATAGCAGATCTGAACGTGGTAGGCGTGTGGCTTCAGCTCCAACTCCACCAAATACAACTCCTTCCCATGTTTAAAATATAGAAATTAGATCCTAATAATGTTTCCTTTACTTGGTGTATTTTGTCTACTGCTTTCGTCATGTATATTTTGACTTTATTCACCTTAAAGCATGTTCTCAAACCAATGAAATGAGTTCATACTTTGTCTGCTAAACGCATCTCTATAATGGTTTTTATTTATTTTATTTATTTTTTAAATTTATTTATTTTTTATAGTTTTTTAGAA

mRNA sequence

ATTCCATACTTTTAATTAACCGTTTCTCCCGAATCCCACTCCAATATAAATACCACAACTCATATCAAACAAATTACAAGATCATTACCTACTAATTGTTTCATTTTGTTGATTGAAAAGTAAAGAGAAAGAAGGAAGGAAAATGGGAGATGTAGAAGGTTCCCCAGGAAGCTCTATGCATGGGGTGACCGGAAGAGAGCAAAGTTTTGCATTTTCCGTAGCTTCCCCAATTGTCCCAACGGACACAACCGCGAAATTTGCATTACCCGTTGACTCTGAGCACAAAGCGAAGGTTTTCAAGGTGTGGTCTTTAGCCAACCCCCACATGAGAACCTTCCACCTTTCTTGGATTTCCTTCTTTACATGCTTCGTCTCCACATTCGCAGCCGCCCCTCTGGTCCCCATCATTCGAGACAATCTCAACCTCACCAAAGTTGACATCGGTAACGCCGGAGTCACCTCCGTGTCTGGCAGTATCTTCTCTCGTCTTGTAATGGGGGCTGTGTGTGACCTTCTGGGGCCACGCTATGGGTGTGCCTTTCTCATTATGCTCTCCGCCCCCACCGTGTTTTGTATGTCCTTTGTGTCCAACGCTGCTGGGTATATAGCTGTGAGATTCATGATTGGCTTCTCGCTTGCCACCTTCGTCTCCTGCCAGTATTGGATGAGCACCATGTTCAACAGTCAGATCATTGGGCTTGTGAATGGGACTGCTGCTGGGTGGGGGAATATGGGCGGAGGAGCCACTCAGCTCATCATGCCTTTGTTGTACGATGTCATTCAGAGAGCTGGCGCTACTCCTTTCACTGCTTGGAGGATTGCCTTCTTTATCCCTGGTTCCCTTCATATTCTCATGGGCATTTTGGTCTTAACTCTTGGCCAGGACTTGCCTGATGGTAACCTTACCAGTCTTCAGAAGAAGGGCAATGTTGCCAAAGATAAATTCTCCAATATCCTGTGGTACGCTGTCACCAATTACCGAACTTGGATCTTCGTCCTCCTCTATGGCTACTCCATGGGCGTCGAACTTTCCACCGATAACGTCATTGCCGAATACTTCTACGACAGGTTTGATCTAAAGCTACACACAGCAGGAATTATCGCAGCAACGTTCGGGATGGCGAATCTAGTGGCTCGACCGTTCGGGGGGTATGCATCGGACGTGGCAGCGCGGTACTTCGGGATGAGAGGAAGGCTGTGGACTTTGTGGATCCTACAAACATGTGGGGGAGTGTTTTGCATGTGGCTTGGGAAAGCAAACAAACTGCCAACAGCAATAGTTGCAATGATATTGTTCTCAATAGGAGCACAGGCTGCATGTGGAGCAACATTTGGGATCATTCCCTTCATTTCTCGACGGTCTTTAGGAATTATATCAGGACTGACAGGAGCAGGAGGAAACTTTGGAAGTGGTTTGACACAATTAGTGTTCTTCTCAACCACCAAATACTCAACAGACACAGGCTTGTTCTTGATGGGAATAATGATTGTATGTTGCACTTTACCAGTCAGTTTGGTTCATTTCCCTCAATGGGGAAGCATGTTTTTACCTCCCACGAAACATGTTGAAAAATCCACTGAGGAATTTTATTATGGTTCTGAATGGACTGAGGATGAAAAGAAGAAGGGTTTGCATCAACAAAGCATTAAGTTTGCTGAGAATAGCAGATCTGAACGTGGTAGGCGTGTGGCTTCAGCTCCAACTCCACCAAATACAACTCCTTCCCATGTTTAAAATATAGAAATTAGATCCTAATAATGTTTCCTTTACTTGGTGTATTTTGTCTACTGCTTTCGTCATGTATATTTTGACTTTATTCACCTTAAAGCATGTTCTCAAACCAATGAAATGAGTTCATACTTTGTCTGCTAAACGCATCTCTATAATGGTTTTTATTTATTTTATTTATTTTTTAAATTTATTTATTTTTTATAGTTTTTTAGAA

Coding sequence (CDS)

ATGGGAGATGTAGAAGGTTCCCCAGGAAGCTCTATGCATGGGGTGACCGGAAGAGAGCAAAGTTTTGCATTTTCCGTAGCTTCCCCAATTGTCCCAACGGACACAACCGCGAAATTTGCATTACCCGTTGACTCTGAGCACAAAGCGAAGGTTTTCAAGGTGTGGTCTTTAGCCAACCCCCACATGAGAACCTTCCACCTTTCTTGGATTTCCTTCTTTACATGCTTCGTCTCCACATTCGCAGCCGCCCCTCTGGTCCCCATCATTCGAGACAATCTCAACCTCACCAAAGTTGACATCGGTAACGCCGGAGTCACCTCCGTGTCTGGCAGTATCTTCTCTCGTCTTGTAATGGGGGCTGTGTGTGACCTTCTGGGGCCACGCTATGGGTGTGCCTTTCTCATTATGCTCTCCGCCCCCACCGTGTTTTGTATGTCCTTTGTGTCCAACGCTGCTGGGTATATAGCTGTGAGATTCATGATTGGCTTCTCGCTTGCCACCTTCGTCTCCTGCCAGTATTGGATGAGCACCATGTTCAACAGTCAGATCATTGGGCTTGTGAATGGGACTGCTGCTGGGTGGGGGAATATGGGCGGAGGAGCCACTCAGCTCATCATGCCTTTGTTGTACGATGTCATTCAGAGAGCTGGCGCTACTCCTTTCACTGCTTGGAGGATTGCCTTCTTTATCCCTGGTTCCCTTCATATTCTCATGGGCATTTTGGTCTTAACTCTTGGCCAGGACTTGCCTGATGGTAACCTTACCAGTCTTCAGAAGAAGGGCAATGTTGCCAAAGATAAATTCTCCAATATCCTGTGGTACGCTGTCACCAATTACCGAACTTGGATCTTCGTCCTCCTCTATGGCTACTCCATGGGCGTCGAACTTTCCACCGATAACGTCATTGCCGAATACTTCTACGACAGGTTTGATCTAAAGCTACACACAGCAGGAATTATCGCAGCAACGTTCGGGATGGCGAATCTAGTGGCTCGACCGTTCGGGGGGTATGCATCGGACGTGGCAGCGCGGTACTTCGGGATGAGAGGAAGGCTGTGGACTTTGTGGATCCTACAAACATGTGGGGGAGTGTTTTGCATGTGGCTTGGGAAAGCAAACAAACTGCCAACAGCAATAGTTGCAATGATATTGTTCTCAATAGGAGCACAGGCTGCATGTGGAGCAACATTTGGGATCATTCCCTTCATTTCTCGACGGTCTTTAGGAATTATATCAGGACTGACAGGAGCAGGAGGAAACTTTGGAAGTGGTTTGACACAATTAGTGTTCTTCTCAACCACCAAATACTCAACAGACACAGGCTTGTTCTTGATGGGAATAATGATTGTATGTTGCACTTTACCAGTCAGTTTGGTTCATTTCCCTCAATGGGGAAGCATGTTTTTACCTCCCACGAAACATGTTGAAAAATCCACTGAGGAATTTTATTATGGTTCTGAATGGACTGAGGATGAAAAGAAGAAGGGTTTGCATCAACAAAGCATTAAGTTTGCTGAGAATAGCAGATCTGAACGTGGTAGGCGTGTGGCTTCAGCTCCAACTCCACCAAATACAACTCCTTCCCATGTTTAA

Protein sequence

MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGILVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTCGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEEFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV
Homology
BLAST of CmUC03G052590 vs. NCBI nr
Match: XP_038895485.1 (high affinity nitrate transporter 2.4-like [Benincasa hispida] >XP_038895611.1 high affinity nitrate transporter 2.4-like [Benincasa hispida])

HSP 1 Score: 1040.4 bits (2689), Expect = 5.3e-300
Identity = 519/530 (97.92%), Postives = 525/530 (99.06%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKF+LPVDSEHKAKVFK+WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFSLPVDSEHKAKVFKLWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPG LH+LMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNV KDKFSN+LWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLGSLQKKGNVTKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTDTGLF MGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFFMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 530

BLAST of CmUC03G052590 vs. NCBI nr
Match: XP_031736393.1 (high affinity nitrate transporter 2.4-like isoform X1 [Cucumis sativus] >AZP53714.1 high affinity nitrate transporter 2.1 [Cucumis sativus] >KAE8652752.1 hypothetical protein Csa_014056 [Cucumis sativus])

HSP 1 Score: 1036.2 bits (2678), Expect = 9.9e-299
Identity = 516/530 (97.36%), Postives = 524/530 (98.87%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPG LH+LMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNVAKDKFSN+LWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTP+HV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

BLAST of CmUC03G052590 vs. NCBI nr
Match: NP_001274401.1 (high affinity nitrate transporter 2.4-like [Cucumis sativus] >AAS93686.3 high-affinity nitrate transporter [Cucumis sativus])

HSP 1 Score: 1034.2 bits (2673), Expect = 3.8e-298
Identity = 516/530 (97.36%), Postives = 523/530 (98.68%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPG LH+LMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNVAKDKFSN+LWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPS V
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSRV 530

BLAST of CmUC03G052590 vs. NCBI nr
Match: AGO64298.1 (high-affinity nitrate transporter NRT2.2 [Cucumis sativus])

HSP 1 Score: 1033.9 bits (2672), Expect = 4.9e-298
Identity = 514/530 (96.98%), Postives = 524/530 (98.87%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVS+AAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPG LH+LMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNVAKDKFSN+LWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTP+HV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

BLAST of CmUC03G052590 vs. NCBI nr
Match: NP_001269063.2 (high affinity nitrate transporter 2.4-like [Cucumis sativus])

HSP 1 Score: 1030.0 bits (2662), Expect = 7.1e-297
Identity = 513/530 (96.79%), Postives = 522/530 (98.49%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVS+AAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPG LH+LMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNVAKDKFSN+LWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLW LWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWALWILQT 360

Query: 361 CGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPS V
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSRV 530

BLAST of CmUC03G052590 vs. ExPASy Swiss-Prot
Match: O82811 (High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 3.0e-250
Identity = 425/530 (80.19%), Postives = 472/530 (89.06%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGD  G PGSSMHGVTGREQSFAFSV SPIV TD TAKF LPVD+EHKA VFK++S A P
Sbjct: 1   MGDSTGEPGSSMHGVTGREQSFAFSVQSPIVHTDKTAKFDLPVDTEHKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISF TCFVSTFAAAPLVPIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFVS+AAG+I VRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF+PG LHI+MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVL LGQDLPDGN  +L+K G VAKDKF  ILWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYF+DRF LKLHTAG+IAA FGMAN  ARP GGYASD AA+YFGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GG+FC+WLG+AN L TA+VAM+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGN
Sbjct: 361 AGGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQL+FFST+ ++T+ GL  MG+MIV CTLPV+LVHFPQWGSMFLPP+    K TE
Sbjct: 421 FGSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
             YYGSEW E EK+K +HQ S++FAEN++SE GRRV SA TPP  TP++V
Sbjct: 481 AHYYGSEWNEQEKQKNMHQGSLRFAENAKSEGGRRVRSAATPPENTPNNV 530

BLAST of CmUC03G052590 vs. ExPASy Swiss-Prot
Match: Q9FJH8 (High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 PE=2 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 8.9e-250
Identity = 422/530 (79.62%), Postives = 474/530 (89.43%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           M D  G PGSSMHGVTGREQS+AFSV SP VP+D++AKF+LPVD+EHKAKVFK+ S   P
Sbjct: 1   MADGFGEPGSSMHGVTGREQSYAFSVESPAVPSDSSAKFSLPVDTEHKAKVFKLLSFEAP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHL+WISFFTCF+STFAAAPLVPIIRDNLNLT+ D+GNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFV  A GYI VRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
            QIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWR+AFF+PG +HI+MGI
Sbjct: 181 GQIIGLVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGN ++L+KKG V KDKFS +LWYA+TNYRTW+FVLLYGYSMGVEL+TDN
Sbjct: 241 LVLTLGQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYF+DRF LKLHTAGIIAA+FGMAN  ARP GG+ASD+AAR FGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GG FC+WLG+A  LPTA+V MILFS+GAQAACGATF IIPFISRRSLGIISGLTGAGGN
Sbjct: 361 LGGFFCLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFST+ +ST+ GL  MG+MI+ CTLPV+LVHFPQWGSMFLP T+   KSTE
Sbjct: 421 FGSGLTQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           E+YY  EWTE EK+KG+H+ S+KFA NSRSERGRRVASAP+PP   P HV
Sbjct: 481 EYYYMKEWTETEKRKGMHEGSLKFAVNSRSERGRRVASAPSPP---PEHV 527

BLAST of CmUC03G052590 vs. ExPASy Swiss-Prot
Match: P0DKG9 (High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 6.0e-238
Identity = 406/529 (76.75%), Postives = 458/529 (86.58%), Query Frame = 0

Query: 6   GSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANPHMRTF 65
           G+PGSS+HGVTGRE +FAFS           +KF LPVDSEHKAK  ++ S ANPHMRTF
Sbjct: 7   GAPGSSLHGVTGREPAFAFSTEVGGEDAAAASKFDLPVDSEHKAKTIRLLSFANPHMRTF 66

Query: 66  HLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLL 125
           HLSWISFF+CFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRL MGA+CD+L
Sbjct: 67  HLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAICDML 126

Query: 126 GPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIG 185
           GPRYGCAFLIML+APTVFCMS + +AAGYIAVRF+IGFSLATFVSCQYWMSTMFNS+IIG
Sbjct: 127 GPRYGCAFLIMLAAPTVFCMSLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIG 186

Query: 186 LVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGILVLTL 245
           LVNG AAGWGNMGGGATQLIMPL+YDVI++ GATPFTAWR+A+F+PG+LH++MG+LVLTL
Sbjct: 187 LVNGLAAGWGNMGGGATQLIMPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVLVLTL 246

Query: 246 GQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEY 305
           GQDLPDGNL SLQKKG+V +D FS +LWYAVTNYRTWIFVLLYGYSMGVEL+TDNVIAEY
Sbjct: 247 GQDLPDGNLRSLQKKGDVNRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEY 306

Query: 306 FYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTCGGVF 365
           FYDRFDL L  AGIIAA+FGMAN+VARP GG  SD+ ARYFGMR RLW +WILQT GG F
Sbjct: 307 FYDRFDLDLRVAGIIAASFGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAF 366

Query: 366 CMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGL 425
           C+ LG+A+ LPT++V M+LFS  AQAACGA FG+IPF+SRRSLGIISG+TGAGGNFG+GL
Sbjct: 367 CLLLGRASTLPTSVVCMVLFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNFGAGL 426

Query: 426 TQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEEFYYG 485
           TQL+FF++++YST TGL  MGIMI+ CTLPV LVHFPQWGSMFLPP    E   EE YYG
Sbjct: 427 TQLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAE---EEHYYG 486

Query: 486 SEWTEDEKKKGLHQQSIKFAENSRSERGRR-----VASAPTPPNTTPSH 530
           SEW+E EK KGLH  S+KFAENSRSERGRR      A+A TPPN +P H
Sbjct: 487 SEWSEQEKSKGLHGASLKFAENSRSERGRRNVINAAAAAATPPNNSPEH 532

BLAST of CmUC03G052590 vs. ExPASy Swiss-Prot
Match: P0DKH0 (High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.2 PE=1 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 6.0e-238
Identity = 406/529 (76.75%), Postives = 458/529 (86.58%), Query Frame = 0

Query: 6   GSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANPHMRTF 65
           G+PGSS+HGVTGRE +FAFS           +KF LPVDSEHKAK  ++ S ANPHMRTF
Sbjct: 7   GAPGSSLHGVTGREPAFAFSTEVGGEDAAAASKFDLPVDSEHKAKTIRLLSFANPHMRTF 66

Query: 66  HLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLL 125
           HLSWISFF+CFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRL MGA+CD+L
Sbjct: 67  HLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAICDML 126

Query: 126 GPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIG 185
           GPRYGCAFLIML+APTVFCMS + +AAGYIAVRF+IGFSLATFVSCQYWMSTMFNS+IIG
Sbjct: 127 GPRYGCAFLIMLAAPTVFCMSLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIG 186

Query: 186 LVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGILVLTL 245
           LVNG AAGWGNMGGGATQLIMPL+YDVI++ GATPFTAWR+A+F+PG+LH++MG+LVLTL
Sbjct: 187 LVNGLAAGWGNMGGGATQLIMPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVLVLTL 246

Query: 246 GQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEY 305
           GQDLPDGNL SLQKKG+V +D FS +LWYAVTNYRTWIFVLLYGYSMGVEL+TDNVIAEY
Sbjct: 247 GQDLPDGNLRSLQKKGDVNRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEY 306

Query: 306 FYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTCGGVF 365
           FYDRFDL L  AGIIAA+FGMAN+VARP GG  SD+ ARYFGMR RLW +WILQT GG F
Sbjct: 307 FYDRFDLDLRVAGIIAASFGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAF 366

Query: 366 CMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGL 425
           C+ LG+A+ LPT++V M+LFS  AQAACGA FG+IPF+SRRSLGIISG+TGAGGNFG+GL
Sbjct: 367 CLLLGRASTLPTSVVCMVLFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNFGAGL 426

Query: 426 TQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEEFYYG 485
           TQL+FF++++YST TGL  MGIMI+ CTLPV LVHFPQWGSMFLPP    E   EE YYG
Sbjct: 427 TQLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAE---EEHYYG 486

Query: 486 SEWTEDEKKKGLHQQSIKFAENSRSERGRR-----VASAPTPPNTTPSH 530
           SEW+E EK KGLH  S+KFAENSRSERGRR      A+A TPPN +P H
Sbjct: 487 SEWSEQEKSKGLHGASLKFAENSRSERGRRNVINAAAAAATPPNNSPEH 532

BLAST of CmUC03G052590 vs. ExPASy Swiss-Prot
Match: Q9LMZ9 (High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana OX=3702 GN=NRT2.2 PE=2 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 3.6e-227
Identity = 390/516 (75.58%), Postives = 452/516 (87.60%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MG  +  PGSSMHGVTGREQS+AFSV     PT+T  K+ LPVD+E KA VFK++S A P
Sbjct: 1   MGSTD-EPGSSMHGVTGREQSYAFSVDGS-EPTNTKKKYNLPVDAEDKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISF TCFVSTFAAAPL+PIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLIPIIRENLNLTKHDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFVS+AAG+I VRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF+PG LHI+MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLP GN  +++K G VAKDKF  ILWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPGGNRAAMEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYF+DRF LKLHTAGIIAA FGMAN  ARP GG+ASD+AA+ FGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGIIAACFGMANFFARPAGGWASDIAAKRFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GG+FC+WLG+AN L TA+V+M+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGN
Sbjct: 361 SGGLFCVWLGRANTLVTAVVSMVLFSLGAQAACGATFAIVPFVSRRALGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFST++++T+ GL  MG+MIV CTLPV+L+HFPQWGSMF PP+     +TE
Sbjct: 421 FGSGLTQLVFFSTSRFTTEEGLTWMGVMIVACTLPVTLIHFPQWGSMFFPPSNDSVDATE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRV 517
            +Y G E++++E++ G+H +S  FA+ +++E G  V
Sbjct: 481 HYYVG-EYSKEEQQIGMHLKSKLFADGAKTEGGSSV 513

BLAST of CmUC03G052590 vs. ExPASy TrEMBL
Match: A0A3Q9CZ42 (High affinity nitrate transporter 2.1 OS=Cucumis sativus OX=3659 GN=NRT2.1 PE=2 SV=1)

HSP 1 Score: 1036.2 bits (2678), Expect = 4.8e-299
Identity = 516/530 (97.36%), Postives = 524/530 (98.87%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPG LH+LMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNVAKDKFSN+LWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTP+HV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

BLAST of CmUC03G052590 vs. ExPASy TrEMBL
Match: Q6PRP8 (High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT2 PE=2 SV=3)

HSP 1 Score: 1034.2 bits (2673), Expect = 1.8e-298
Identity = 516/530 (97.36%), Postives = 523/530 (98.68%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPG LH+LMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNVAKDKFSN+LWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPS V
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSRV 530

BLAST of CmUC03G052590 vs. ExPASy TrEMBL
Match: S4VM42 (High-affinity nitrate transporter NRT2.2 OS=Cucumis sativus OX=3659 GN=NRT2.2 PE=2 SV=1)

HSP 1 Score: 1033.9 bits (2672), Expect = 2.4e-298
Identity = 514/530 (96.98%), Postives = 524/530 (98.87%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVS+AAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPG LH+LMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNVAKDKFSN+LWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTP+HV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

BLAST of CmUC03G052590 vs. ExPASy TrEMBL
Match: A0A6J1HIH1 (high affinity nitrate transporter 2.4-like OS=Cucurbita moschata OX=3662 GN=LOC111464708 PE=4 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 8.8e-293
Identity = 505/530 (95.28%), Postives = 520/530 (98.11%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           M  VEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVFK++S ANP
Sbjct: 1   MSGVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVS+AAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLYD+I+RAGATPFTAWRIAFFIPG LH++MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNL+SLQKKG+VAKDKFS +LWYA+TNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLSSLQKKGDVAKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA+KLPTAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTD GLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYG+EWTEDEKKKGLHQQSIKFAENSRSERGRRV SAPTPPNTTPSHV
Sbjct: 481 EFYYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 530

BLAST of CmUC03G052590 vs. ExPASy TrEMBL
Match: A0A6J1DHB7 (high affinity nitrate transporter 2.4-like OS=Momordica charantia OX=3673 GN=LOC111020453 PE=4 SV=1)

HSP 1 Score: 1013.8 bits (2620), Expect = 2.6e-292
Identity = 500/529 (94.52%), Postives = 520/529 (98.30%), Query Frame = 0

Query: 2   GDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANPH 61
           G+VEGSPGSSMHGVTGREQ+FAFSVASPIVPTDT+AKFALPVDSEHKAKVFK++SLANPH
Sbjct: 6   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 65

Query: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 66  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 125

Query: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS+ AGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 126 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 185

Query: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGIL 241
           QIIGLVNGTAAGWGNMGGGATQL+MPLLY++I++ GATPFTAWRIAFFIPGSLH+LMGIL
Sbjct: 186 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 245

Query: 242 VLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301
           V+TLGQDLPDGNL+SLQKKG VAKDKFSN+LWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 246 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 305

Query: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 361
           +AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQTC
Sbjct: 306 VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 365

Query: 362 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421
           GGVFCMWLGKA +LP AIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 366 GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 425

Query: 422 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 481
           GSGLTQLVFFSTTKYSTD GLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 426 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 485

Query: 482 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGR++ASAPTPPN TPSHV
Sbjct: 486 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 534

BLAST of CmUC03G052590 vs. TAIR 10
Match: AT1G08090.1 (nitrate transporter 2:1 )

HSP 1 Score: 865.5 bits (2235), Expect = 2.2e-251
Identity = 425/530 (80.19%), Postives = 472/530 (89.06%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGD  G PGSSMHGVTGREQSFAFSV SPIV TD TAKF LPVD+EHKA VFK++S A P
Sbjct: 1   MGDSTGEPGSSMHGVTGREQSFAFSVQSPIVHTDKTAKFDLPVDTEHKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISF TCFVSTFAAAPLVPIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFVS+AAG+I VRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF+PG LHI+MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVL LGQDLPDGN  +L+K G VAKDKF  ILWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYF+DRF LKLHTAG+IAA FGMAN  ARP GGYASD AA+YFGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GG+FC+WLG+AN L TA+VAM+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGN
Sbjct: 361 AGGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQL+FFST+ ++T+ GL  MG+MIV CTLPV+LVHFPQWGSMFLPP+    K TE
Sbjct: 421 FGSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
             YYGSEW E EK+K +HQ S++FAEN++SE GRRV SA TPP  TP++V
Sbjct: 481 AHYYGSEWNEQEKQKNMHQGSLRFAENAKSEGGRRVRSAATPPENTPNNV 530

BLAST of CmUC03G052590 vs. TAIR 10
Match: AT5G60770.1 (nitrate transporter 2.4 )

HSP 1 Score: 864.0 bits (2231), Expect = 6.3e-251
Identity = 422/530 (79.62%), Postives = 474/530 (89.43%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           M D  G PGSSMHGVTGREQS+AFSV SP VP+D++AKF+LPVD+EHKAKVFK+ S   P
Sbjct: 1   MADGFGEPGSSMHGVTGREQSYAFSVESPAVPSDSSAKFSLPVDTEHKAKVFKLLSFEAP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHL+WISFFTCF+STFAAAPLVPIIRDNLNLT+ D+GNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFV  A GYI VRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
            QIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWR+AFF+PG +HI+MGI
Sbjct: 181 GQIIGLVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGN ++L+KKG V KDKFS +LWYA+TNYRTW+FVLLYGYSMGVEL+TDN
Sbjct: 241 LVLTLGQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYF+DRF LKLHTAGIIAA+FGMAN  ARP GG+ASD+AAR FGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GG FC+WLG+A  LPTA+V MILFS+GAQAACGATF IIPFISRRSLGIISGLTGAGGN
Sbjct: 361 LGGFFCLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFST+ +ST+ GL  MG+MI+ CTLPV+LVHFPQWGSMFLP T+   KSTE
Sbjct: 421 FGSGLTQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           E+YY  EWTE EK+KG+H+ S+KFA NSRSERGRRVASAP+PP   P HV
Sbjct: 481 EYYYMKEWTETEKRKGMHEGSLKFAVNSRSERGRRVASAPSPP---PEHV 527

BLAST of CmUC03G052590 vs. TAIR 10
Match: AT1G08100.1 (nitrate transporter 2.2 )

HSP 1 Score: 788.9 bits (2036), Expect = 2.6e-228
Identity = 390/516 (75.58%), Postives = 452/516 (87.60%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MG  +  PGSSMHGVTGREQS+AFSV     PT+T  K+ LPVD+E KA VFK++S A P
Sbjct: 1   MGSTD-EPGSSMHGVTGREQSYAFSVDGS-EPTNTKKKYNLPVDAEDKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISF TCFVSTFAAAPL+PIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLIPIIRENLNLTKHDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFVS+AAG+I VRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF+PG LHI+MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLP GN  +++K G VAKDKF  ILWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPGGNRAAMEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYF+DRF LKLHTAGIIAA FGMAN  ARP GG+ASD+AA+ FGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGIIAACFGMANFFARPAGGWASDIAAKRFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GG+FC+WLG+AN L TA+V+M+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGN
Sbjct: 361 SGGLFCVWLGRANTLVTAVVSMVLFSLGAQAACGATFAIVPFVSRRALGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFST++++T+ GL  MG+MIV CTLPV+L+HFPQWGSMF PP+     +TE
Sbjct: 421 FGSGLTQLVFFSTSRFTTEEGLTWMGVMIVACTLPVTLIHFPQWGSMFFPPSNDSVDATE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRV 517
            +Y G E++++E++ G+H +S  FA+ +++E G  V
Sbjct: 481 HYYVG-EYSKEEQQIGMHLKSKLFADGAKTEGGSSV 513

BLAST of CmUC03G052590 vs. TAIR 10
Match: AT5G60780.1 (nitrate transporter 2.3 )

HSP 1 Score: 786.2 bits (2029), Expect = 1.7e-227
Identity = 387/531 (72.88%), Postives = 450/531 (84.75%), Query Frame = 0

Query: 5   EGSPGSSMHGVTGREQSFAFSV---ASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANPH 64
           EGS G+S+HGVT REQ F+FSV   +  +   D TAKFALPVDSEH+AKVF   S A PH
Sbjct: 9   EGSIGTSLHGVTAREQVFSFSVDASSQTVQSDDPTAKFALPVDSEHRAKVFNPLSFAKPH 68

Query: 65  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 124
           MR FHL W+SFFTCF+STFAAAPLVPIIRDNL+LTK DIGNAGV SVSG+IFSRL MGAV
Sbjct: 69  MRAFHLGWLSFFTCFISTFAAAPLVPIIRDNLDLTKTDIGNAGVASVSGAIFSRLAMGAV 128

Query: 125 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 184
           CDLLG RYG AF +ML+APTVF MSFV   +GY+ VRFMIGF LATFVSCQYW S MFN 
Sbjct: 129 CDLLGARYGTAFSLMLTAPTVFSMSFVGGPSGYLGVRFMIGFCLATFVSCQYWTSVMFNG 188

Query: 185 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGIL 244
           +IIGLVNG A GWG+MGGG TQL+MP+++ VI+ AGATPF AWRIAFF+PG L ++MGIL
Sbjct: 189 KIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLAGATPFMAWRIAFFVPGFLQVVMGIL 248

Query: 245 VLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELSTDNV 304
           VL+LGQDLPDGNL++LQK G V+KDKFS + W+AV NYRTWI  +LYG SMG+EL+ +NV
Sbjct: 249 VLSLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFVLYGSSMGIELTINNV 308

Query: 305 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 364
           I+ YFYDRF+LKL TAGI+AA+FGMAN +ARPFGGYASDVAAR FGMRGRLWTLWI QT 
Sbjct: 309 ISGYFYDRFNLKLQTAGIVAASFGMANFIARPFGGYASDVAARVFGMRGRLWTLWIFQTV 368

Query: 365 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 424
           G +FC+WLG+A+ LP AI+AM+LFSIG QAACGA FG+ PF+SRRSLG+ISGLTGAGGNF
Sbjct: 369 GALFCIWLGRASSLPIAILAMMLFSIGTQAACGALFGVAPFVSRRSLGLISGLTGAGGNF 428

Query: 425 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 484
           GSGLTQL+FFS+ ++ST  GL LMG+M V CTLPV+ +HFPQWGSMFL P+   E+S EE
Sbjct: 429 GSGLTQLLFFSSARFSTAEGLSLMGVMAVLCTLPVAFIHFPQWGSMFLRPSTDGERSQEE 488

Query: 485 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASA--PTPPNTTPSHV 531
           +YYGSEWTE+EK++GLH+ SIKFAENSRSERGR+VA A  PTP N TPSHV
Sbjct: 489 YYYGSEWTENEKQQGLHEGSIKFAENSRSERGRKVALANIPTPENGTPSHV 539

BLAST of CmUC03G052590 vs. TAIR 10
Match: AT3G45060.1 (high affinity nitrate transporter 2.6 )

HSP 1 Score: 773.9 bits (1997), Expect = 8.6e-224
Identity = 382/534 (71.54%), Postives = 447/534 (83.71%), Query Frame = 0

Query: 5   EGSPGSSMHGVTGREQSFAFSV------ASPIVPTDTTAKFALPVDSEHKAKVFKVWSLA 64
           +GS G+S+HGVT REQ F+FSV      +  +   D TAKFALPVDSEH+AKVFK  S A
Sbjct: 9   DGSIGTSLHGVTAREQVFSFSVQEDVPSSQAVRTNDPTAKFALPVDSEHRAKVFKPLSFA 68

Query: 65  NPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVM 124
            PHMR FHL WISFFTCF+STFAAAPLVP+IRDNL+LTK DIGNAGV SVSG+IFSRL M
Sbjct: 69  KPHMRAFHLGWISFFTCFISTFAAAPLVPVIRDNLDLTKTDIGNAGVASVSGAIFSRLAM 128

Query: 125 GAVCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTM 184
           GAVCDLLG RYG AF +ML+AP VF MSFV++A  Y+AVRFMIGF LATFVSCQYW S M
Sbjct: 129 GAVCDLLGARYGTAFSLMLTAPAVFSMSFVADAGSYLAVRFMIGFCLATFVSCQYWTSVM 188

Query: 185 FNSQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILM 244
           F  +IIGLVNG A GWG+MGGG TQL+MP+++ VI+  GATPFTAWR AFFIPG L I+M
Sbjct: 189 FTGKIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLTGATPFTAWRFAFFIPGILQIVM 248

Query: 245 GILVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGYSMGVELST 304
           GILVLTLGQDLPDGNL++LQK G V+KDKFS + W+AV NYRTWI  +LYG+SMGVEL+ 
Sbjct: 249 GILVLTLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFMLYGFSMGVELTI 308

Query: 305 DNVIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWIL 364
           +NVI+ YFYDRF+L LHTAGIIAA+FGMAN  ARPFGGYASDVAAR FGMRGRLW LWIL
Sbjct: 309 NNVISGYFYDRFNLTLHTAGIIAASFGMANFFARPFGGYASDVAARLFGMRGRLWILWIL 368

Query: 365 QTCGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAG 424
           QT G +FC+WLG+A+ LP AI+AM+LFS+G QAACGA FG+ PF+SRRSLG+ISGLTGAG
Sbjct: 369 QTVGALFCIWLGRASSLPIAILAMMLFSMGTQAACGALFGVAPFVSRRSLGLISGLTGAG 428

Query: 425 GNFGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKS 484
           GNFGSG+TQL+FFS++++ST  GL LMG+M V C+LPV+ +HFPQWGSMFL P++  EKS
Sbjct: 429 GNFGSGVTQLLFFSSSRFSTAEGLSLMGVMAVVCSLPVAFIHFPQWGSMFLRPSQDGEKS 488

Query: 485 TEEFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRR--VASAPTPPNTTPSHV 531
            EE YYG+EWTE+EK  GLH+ SIKFAENSRSERGR+  +A  PTP   +P+HV
Sbjct: 489 KEEHYYGAEWTEEEKSLGLHEGSIKFAENSRSERGRKAMLADIPTPETGSPAHV 542

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895485.15.3e-30097.92high affinity nitrate transporter 2.4-like [Benincasa hispida] >XP_038895611.1 h... [more]
XP_031736393.19.9e-29997.36high affinity nitrate transporter 2.4-like isoform X1 [Cucumis sativus] >AZP5371... [more]
NP_001274401.13.8e-29897.36high affinity nitrate transporter 2.4-like [Cucumis sativus] >AAS93686.3 high-af... [more]
AGO64298.14.9e-29896.98high-affinity nitrate transporter NRT2.2 [Cucumis sativus][more]
NP_001269063.27.1e-29796.79high affinity nitrate transporter 2.4-like [Cucumis sativus][more]
Match NameE-valueIdentityDescription
O828113.0e-25080.19High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 ... [more]
Q9FJH88.9e-25079.62High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 ... [more]
P0DKG96.0e-23876.75High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
P0DKH06.0e-23876.75High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9LMZ93.6e-22775.58High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana OX=3702 GN=NRT2.2 ... [more]
Match NameE-valueIdentityDescription
A0A3Q9CZ424.8e-29997.36High affinity nitrate transporter 2.1 OS=Cucumis sativus OX=3659 GN=NRT2.1 PE=2 ... [more]
Q6PRP81.8e-29897.36High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT2 PE=2 SV=3[more]
S4VM422.4e-29896.98High-affinity nitrate transporter NRT2.2 OS=Cucumis sativus OX=3659 GN=NRT2.2 PE... [more]
A0A6J1HIH18.8e-29395.28high affinity nitrate transporter 2.4-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1DHB72.6e-29294.52high affinity nitrate transporter 2.4-like OS=Momordica charantia OX=3673 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT1G08090.12.2e-25180.19nitrate transporter 2:1 [more]
AT5G60770.16.3e-25179.62nitrate transporter 2.4 [more]
AT1G08100.12.6e-22875.58nitrate transporter 2.2 [more]
AT5G60780.11.7e-22772.88nitrate transporter 2.3 [more]
AT3G45060.18.6e-22471.54high affinity nitrate transporter 2.6 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 58..254
e-value: 4.7E-21
score: 77.1
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 264..464
e-value: 3.7E-27
score: 97.0
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 58..459
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 69..425
e-value: 6.2E-20
score: 71.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 490..513
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 490..530
NoneNo IPR availablePANTHERPTHR23515:SF16HIGH-AFFINITY NITRATE TRANSPORTER 2.1-LIKEcoord: 1..527
IPR044772Nitrate transporterPANTHERPTHR23515HIGH-AFFINITY NITRATE TRANSPORTER 2.3coord: 1..527
IPR044772Nitrate transporterCDDcd17341MFS_NRT2_likecoord: 65..458
e-value: 1.36699E-135
score: 396.237

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC03G052590.1CmUC03G052590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071249 cellular response to nitrate
biological_process GO:0042128 nitrate assimilation
biological_process GO:0015706 nitrate transport
biological_process GO:0031204 posttranslational protein targeting to membrane, translocation
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0071261 Ssh1 translocon complex
molecular_function GO:0015112 nitrate transmembrane transporter activity
molecular_function GO:0008320 protein transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity