Homology
BLAST of Clc07G03720 vs. NCBI nr
Match:
XP_038892474.1 (cucumisin-like [Benincasa hispida])
HSP 1 Score: 1309.3 bits (3387), Expect = 0.0e+00
Identity = 659/745 (88.46%), Postives = 692/745 (92.89%), Query Frame = 0
Query: 3 MTRNTMSSSLIFKLVVLLGLISSLFASSFNSNNDDGRKIYIVYMGNKPKDAASTSSHHMR 62
MT NTMSSSLIFKLVV+L L+SSL SS +S+ DGRK+YIVYMGNKPKD AST SHHMR
Sbjct: 1 MTTNTMSSSLIFKLVVVLSLVSSLLVSSSDSDK-DGRKVYIVYMGNKPKDTASTPSHHMR 60
Query: 63 MLEEVIGSNFAPEALLHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTTRS 122
ML+EV GSNFAPE+LLHSYKRSFNGFVVKLTEEEA ISAKEGVVSVFPNGKKH+HTTRS
Sbjct: 61 MLKEVTGSNFAPESLLHSYKRSFNGFVVKLTEEEARTISAKEGVVSVFPNGKKHLHTTRS 120
Query: 123 WDFMGFTKDVPRVNQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNFTC 182
WDF+GFTKDVPRV QVES+IVVGVLD+GIWPE+PSFSD GYGPPPAKWKGTCQTSTNFTC
Sbjct: 121 WDFIGFTKDVPRVKQVESNIVVGVLDSGIWPENPSFSDFGYGPPPAKWKGTCQTSTNFTC 180
Query: 183 NKKIIGARAYRSDNFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARGGV 242
N KIIGARAYRSDN FP QDIQSPRDS+GHGTHTASTVAGGLVNQASLYGLALGTARGGV
Sbjct: 181 NNKIIGARAYRSDNDFPTQDIQSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGV 240
Query: 243 PSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAFHS 302
PSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGG+E RYYFNDSIAIGAFHS
Sbjct: 241 PSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGNEARYYFNDSIAIGAFHS 300
Query: 303 MKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTINTF 362
MKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVS+VQLGN NIYQGYTINTF
Sbjct: 301 MKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSRVQLGNRNIYQGYTINTF 360
Query: 363 DLLEKQYPLIYAGDAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIYFS 422
DL KQYPLIYAGDAPNI GGFTGSISRFCS NS+D +LV GKILLCDS L PS+F+YFS
Sbjct: 361 DLGGKQYPLIYAGDAPNIAGGFTGSISRFCSENSIDSSLVSGKILLCDSVLPPSSFVYFS 420
Query: 423 NAAGVVMNDAGVKDRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDTSAP 482
+AAGVVMND GVK+ +NSYPLPSSYLET DGN IKTYM SNGVPTATILKS AVNDTSAP
Sbjct: 421 DAAGVVMNDNGVKNPSNSYPLPSSYLETVDGNAIKTYMASNGVPTATILKSSAVNDTSAP 480
Query: 483 LIVSFSSRGPNPETLDILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISGTSMS 542
IVSFSSRGPNPETLDILKPDLTAPGVEILAAW PIAP+SSGVIDSR +MYNIISGTSMS
Sbjct: 481 FIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPISSGVIDSRTSMYNIISGTSMS 540
Query: 543 CPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNIQAEFAYGAGHINPLQAINPG 602
CPHATAAAVYVKTFHPTWSPAAIKSALMTTA PLN ELN+QAEFAYGAG INPL+AI+PG
Sbjct: 541 CPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNPELNVQAEFAYGAGQINPLKAISPG 600
Query: 603 LVYDANENDYVKFLCGQNYTTAMVQRLSNDNSICNSANFGRVWDLNYPSFALSSTPSEFI 662
LVYDANE DYVKFLCGQ YTTAMVQRLSNDNSIC+SAN GRVWDLNYPSFALSSTPS+ I
Sbjct: 601 LVYDANEFDYVKFLCGQGYTTAMVQRLSNDNSICSSANSGRVWDLNYPSFALSSTPSQSI 660
Query: 663 NQIFTRTLTNVESKASTYTSTVLGAPQGLIITVNPQVLSFDGIGQTKSFTLTIRGTIS-Q 722
NQ F RTLTNVESKASTY ST+LGAPQGL ITVNPQVLSF G G+ KSFTLTIRGTI+
Sbjct: 661 NQFFRRTLTNVESKASTYRSTILGAPQGLTITVNPQVLSFSGFGEKKSFTLTIRGTINPP 720
Query: 723 SIVSASLVWSDSSHNVRSPITVYIV 747
SIVSASLVWSD SHNVRSPITVY+V
Sbjct: 721 SIVSASLVWSDGSHNVRSPITVYVV 744
BLAST of Clc07G03720 vs. NCBI nr
Match:
XP_011656180.2 (cucumisin [Cucumis sativus] >KAE8648002.1 hypothetical protein Csa_021416 [Cucumis sativus])
HSP 1 Score: 1280.4 bits (3312), Expect = 0.0e+00
Identity = 642/747 (85.94%), Postives = 686/747 (91.83%), Query Frame = 0
Query: 3 MTRNTMSSSLIFK--LVVLLGLISSLFASSFNSNNDDGRKIYIVYMGNKPKDAASTSSHH 62
MTRN MSSSLIFK LV++LGL+SSL AS F+S NDD RKIYIVYMGNKP+D AST SHH
Sbjct: 1 MTRNIMSSSLIFKLALVLVLGLVSSLLASGFDSKNDD-RKIYIVYMGNKPQDTASTPSHH 60
Query: 63 MRMLEEVIGSNFAPEALLHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTT 122
MRML EV GSNFAPE+LLHSYKRSFNGFVVKLTEEEAH+ISAKEGVVSVFP+GKKH+HTT
Sbjct: 61 MRMLREVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTT 120
Query: 123 RSWDFMGFTKDVPRVNQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNF 182
RSWDF+GFTKDVPRVNQVESDIVVGVLD+GIWPE+PSFSD GYGP PAKWKG CQ TNF
Sbjct: 121 RSWDFIGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNF 180
Query: 183 TCNKKIIGARAYRSDNFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARG 242
TCNKKIIGARAYRSDN FP +DI SPRDS GHGTHTASTVAGGLV+QASLYGLALGTARG
Sbjct: 181 TCNKKIIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARG 240
Query: 243 GVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAF 302
GVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSE RYYFNDSIAIGAF
Sbjct: 241 GVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAF 300
Query: 303 HSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTIN 362
HSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAAST DRKLVS+V++GN N+YQGYTIN
Sbjct: 301 HSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTIN 360
Query: 363 TFDLLEKQYPLIYAGDAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIY 422
TFD L KQYPLIYAGDAPN+ GGFTGSISRFCS SVD NLV GKILLCDS LAPS F+Y
Sbjct: 361 TFDPLGKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVY 420
Query: 423 FSNAAGVVMNDAGVKDRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDTS 482
FS+A GVVMND GVK +NSYPLPSSYLET DG+ IKTYM SNGVPTATI KSDAVND+S
Sbjct: 421 FSDAVGVVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSS 480
Query: 483 APLIVSFSSRGPNPETLDILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISGTS 542
AP IVSFSSRGPNPETLDILKPDLTAPGVEILAAW PIAPVSSGVIDSR T+YNIISGTS
Sbjct: 481 APFIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTS 540
Query: 543 MSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNIQAEFAYGAGHINPLQAIN 602
MSCPHATAAAVYVKTFHPTWSPAAIKSALMTTA PL E+N++AEFAYGAG INPL+AI+
Sbjct: 541 MSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAIS 600
Query: 603 PGLVYDANENDYVKFLCGQNYTTAMVQRLSNDNSICNSANFGRVWDLNYPSFALSSTPSE 662
PGLVYDANE DYVKFLCGQ YT+ MVQ LSNDN+ICNSAN GRVWDLNYPSFALSSTPS+
Sbjct: 601 PGLVYDANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQ 660
Query: 663 FINQIFTRTLTNVESKASTYTSTVLGAPQGLIITVNPQVLSFDGIGQTKSFTLTIRGTIS 722
INQ FTRTLT+V+S ASTYTST+LGAPQGL ITVNP+VLSF GIG+ K+FTLTI+GTI
Sbjct: 661 SINQFFTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSGIGEKKTFTLTIQGTID 720
Query: 723 -QSIVSASLVWSDSSHNVRSPITVYIV 747
+IVSASLVWSDSSH+VRSPIT+Y+V
Sbjct: 721 PTTIVSASLVWSDSSHDVRSPITIYVV 746
BLAST of Clc07G03720 vs. NCBI nr
Match:
XP_008461725.1 (PREDICTED: cucumisin-like [Cucumis melo])
HSP 1 Score: 1266.9 bits (3277), Expect = 0.0e+00
Identity = 634/750 (84.53%), Postives = 683/750 (91.07%), Query Frame = 0
Query: 1 MAMTRNTMSSSLIFK--LVVLLGLISSLFASSFNSNNDDGRKIYIVYMGNKPKDAASTSS 60
+ MTRN MSSSLIFK LV++LGL+ SL AS +S N D RKIYIVYMGNKP+D AST S
Sbjct: 7 LVMTRNIMSSSLIFKLALVLVLGLVFSLLASGLDSENVD-RKIYIVYMGNKPQDTASTPS 66
Query: 61 HHMRMLEEVIGSNFAPEALLHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVH 120
HHMRML EV GSNFAPE+LLHSYKRSFNGFVVKLTEEEAH+ISAKEGVVSVFP+GKKH+H
Sbjct: 67 HHMRMLREVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLH 126
Query: 121 TTRSWDFMGFTKDVPRVNQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTST 180
TTRSWDF+GFTKDVPRVNQVESDIVVGVLD+GIWPE+PSFSDVGYGP PAKWKG+CQTS
Sbjct: 127 TTRSWDFLGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDVGYGPIPAKWKGSCQTSA 186
Query: 181 NFTCNKKIIGARAYRSDNFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTA 240
NFTCNKKIIGARAYRSDN FP DI SPRDS GHGTHTASTVAGGLV+QASLYGLALGTA
Sbjct: 187 NFTCNKKIIGARAYRSDNVFPTADIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTA 246
Query: 241 RGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIG 300
RGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSE RYYFNDSIAIG
Sbjct: 247 RGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIG 306
Query: 301 AFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYT 360
AFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAAST DRKLVS+V++GN N+YQGYT
Sbjct: 307 AFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYT 366
Query: 361 INTFDLLEKQYPLIYAGDAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTF 420
INTFD L KQYPLIYAGDAPNI GGFTGSISRFCS SVD NLV GKILLC S +APS F
Sbjct: 367 INTFDPLGKQYPLIYAGDAPNIDGGFTGSISRFCSEGSVDANLVSGKILLCVSIVAPSAF 426
Query: 421 IYFSNAAGVVMNDAGVKDRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVND 480
+YFS A GVVMND GVK +NSYPLPSSYL+T DG+ IKTYM SNGVPTATI KSDAVND
Sbjct: 427 VYFSEAVGVVMNDDGVKYPSNSYPLPSSYLQTVDGDAIKTYMASNGVPTATIFKSDAVND 486
Query: 481 TSAPLIVSFSSRGPNPETLDILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISG 540
+ AP +VSFSSRGPNPETLDILKPDLTAPG EILA W PIAPVSSGVIDSR TMYNIISG
Sbjct: 487 SYAPFVVSFSSRGPNPETLDILKPDLTAPGAEILAGWSPIAPVSSGVIDSRTTMYNIISG 546
Query: 541 TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNIQAEFAYGAGHINPLQA 600
TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTA PL ++N++AEFAYGAG INPL+A
Sbjct: 547 TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPDINVEAEFAYGAGQINPLKA 606
Query: 601 INPGLVYDANENDYVKFLCGQNYTTAMVQRLSNDNSICNSANFGRVWDLNYPSFALSSTP 660
INPGLVYDANE+DYVKFLCGQ YT+AMVQ LSNDN++CNSAN GRVWDLNYPSFALSSTP
Sbjct: 607 INPGLVYDANESDYVKFLCGQGYTSAMVQSLSNDNTLCNSANIGRVWDLNYPSFALSSTP 666
Query: 661 SEFINQIFTRTLTNVESKASTYTSTVLGAPQGLIITVNPQVLSFDGIGQTKSFTLTIRGT 720
S+ INQ FTRTLT+V+S ASTYTST+LGAPQGL ITVNP+VLSF G+G+ +FTLTI+G+
Sbjct: 667 SQSINQFFTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSGMGEKNTFTLTIQGS 726
Query: 721 IS-QSIVSASLVWSDSSHNVRSPITVYIVN 748
I SIVSASLVWSDSSH+VRSPIT+Y+V+
Sbjct: 727 IDPTSIVSASLVWSDSSHDVRSPITIYVVS 755
BLAST of Clc07G03720 vs. NCBI nr
Match:
XP_022941974.1 (cucumisin-like [Cucurbita moschata])
HSP 1 Score: 1245.3 bits (3221), Expect = 0.0e+00
Identity = 622/749 (83.04%), Postives = 677/749 (90.39%), Query Frame = 0
Query: 1 MAMTRNTMSSSLIFKLVVLLGLISSLFASSFNSNNDDGRKIYIVYMGNKPKDAASTSSHH 60
M M N SSSLIFKLVV L L+ L ASSF+S N DGRK+YIVY+GNK +D AST SHH
Sbjct: 39 MIMAGNNASSSLIFKLVV-LNLVCCLLASSFDSGN-DGRKVYIVYLGNKREDTASTPSHH 98
Query: 61 MRMLEEVIGSNFAPEALLHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTT 120
RMLEE IGS FAPEALLHSYKRSFNGFVV+LTEEEA KISAKEGVVSVFPN KKH HTT
Sbjct: 99 TRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKEGVVSVFPNEKKHPHTT 158
Query: 121 RSWDFMGFTKDVPRVNQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNF 180
RSWDFMGFT +VPRV QVESDIVVGVLDTGIWPESPSF+DVGYGPPPAKWKG CQTSTNF
Sbjct: 159 RSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFNDVGYGPPPAKWKGACQTSTNF 218
Query: 181 TCNKKIIGARAYRSDNFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARG 240
CNKKIIGARAYRSDN FPP+D +SPRDSEGHGTHTASTVAGGLV+QASL+GLALGTARG
Sbjct: 219 RCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARG 278
Query: 241 GVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAF 300
GVPSARIAVYKICWSDGC DADILAAFDDAIADGVDIISLSVGG+EP++YFNDSIAIGAF
Sbjct: 279 GVPSARIAVYKICWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAF 338
Query: 301 HSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTIN 360
HSMK+GILTSNSAGNDGPD+ TIRNFSPWSLSVAAS+IDRKLVS+VQLGN NI+QG+TIN
Sbjct: 339 HSMKNGILTSNSAGNDGPDFLTIRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTIN 398
Query: 361 TFDLLEKQYPLIYAGDAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIY 420
TFDL +KQ+PLIYAG+APNI+GGFTG+ SRFCS NSVD NLV+GKIL+CDS ++PSTF
Sbjct: 399 TFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSIVSPSTFAS 458
Query: 421 FSNAAGVVMNDAGVKDRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDTS 480
FS+A GVVMNDAGVKD A SYPLPSSYL T GN+IKTYM SN PTATI KS+AVNDTS
Sbjct: 459 FSSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTS 518
Query: 481 APLIVSFSSRGPNPETLDILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISGTS 540
AP++VSFSSRGPNPETLDILKPDLTAPGVEILAAWPP APVSSG+ DSR T+YNIISGTS
Sbjct: 519 APIVVSFSSRGPNPETLDILKPDLTAPGVEILAAWPPDAPVSSGIRDSRSTLYNIISGTS 578
Query: 541 MSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNIQAEFAYGAGHINPLQAIN 600
MSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLN LN QAEFAYGAGHI+PL+A N
Sbjct: 579 MSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATN 638
Query: 601 PGLVYDANENDYVKFLCGQNYTTAMVQRLSNDNSICNSANFGRVWDLNYPSFALSSTPSE 660
PGLVYDANE DYV FLCGQ Y+TAMV+RL+ D S+C +AN GRVWDLNYPSFALS+TPSE
Sbjct: 639 PGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSE 698
Query: 661 FINQIFTRTLTNVESKASTYTSTVLGAPQGLIITVNPQVLSFDGIGQTKSFTLTIRGTIS 720
INQ FTRTLTNV+++ASTYTS +LGAP+GL ITV+P LSF+GIG KSFTLTI GTI
Sbjct: 699 SINQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPALSFNGIGDKKSFTLTIDGTII 758
Query: 721 QSIVSASLVWSDSSHNVRSPITVYIVNKA 750
Q+IVSAS+VWSD SHNVRSPIT+YIVNKA
Sbjct: 759 QTIVSASVVWSDGSHNVRSPITIYIVNKA 785
BLAST of Clc07G03720 vs. NCBI nr
Match:
XP_022979186.1 (cucumisin-like [Cucurbita maxima])
HSP 1 Score: 1245.0 bits (3220), Expect = 0.0e+00
Identity = 614/742 (82.75%), Postives = 674/742 (90.84%), Query Frame = 0
Query: 8 MSSSLIFKLVVLLGLISSLFASSFNSNNDDGRKIYIVYMGNKPKDAASTSSHHMRMLEEV 67
MSSSLIFKLV+ + SL ASS +S+++DGRK+YIVY+GNKP+D+AST SHHMRMLEEV
Sbjct: 1 MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
Query: 68 IGSNFAPEALLHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTTRSWDFMG 127
+GS FAP+ALLHSYKRSFNGFVVKLTEEEA KISAKEGVVSVFPNGKKH+HTTRSWDFMG
Sbjct: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
Query: 128 FTKDVPRVNQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNFTCNKKII 187
FTK V RV QVES+IVVGVLD+GIWPESPSFSDVGYGPPPAKWKG CQTS NF CN+KII
Sbjct: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
Query: 188 GARAYRSDNFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 247
GARAYRSDN FPP+DI+SPRDS+GHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
Query: 248 AVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAFHSMKHGI 307
AVYKICWSDGC DADILAAFDDAIADGVDIISLSVGG++P+YYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
Query: 308 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTINTFDLLEK 367
LTSNSAGNDGPDYFTIRNFSPWSLSVAAS+IDRKLVS+VQLGN N+YQGYTINTFDL K
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
Query: 368 QYPLIYAGDAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIYFSNAAGV 427
QYPLIYAG+APN++GGFTGS SRFCS NSVD NLVRGKILLCDS L+PSTF F+ A GV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
Query: 428 VMNDAGVKDRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDTSAPLIVSF 487
VMNDAGVKD + SYPLPSSYL GN+IKTYM SN PTATI KS+AVNDTSAPLIVSF
Sbjct: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
Query: 488 SSRGPNPETLDILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISGTSMSCPHAT 547
SSRGPNPET DILKPDLTAPGVEILAAW PIA VSSGV DSR T+YNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
Query: 548 AAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNIQAEFAYGAGHINPLQAINPGLVYDA 607
AAAVYVKTFHP+WSPAAIKSALMTTAIPLN +LN QAEFAYGAGHINP++A+NPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
Query: 608 NENDYVKFLCGQNYTTAMVQRLSNDNSICNSANFGRVWDLNYPSFALSSTPSEFINQIFT 667
E+DYV+FLCGQ YTTAMV+RLS D+S+C AN GRVWDLNYPSFALSSTPSE INQ F
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
Query: 668 RTLTNVESKASTYTSTVLGAPQGLIITVNPQVLSFDGIGQTKSFTLTIRGTISQSIVSAS 727
RT+TNV SK +TY + VLGAP+GL I+VNP LSF+ IGQ KSFT+T+RG ++Q IVSA+
Sbjct: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
Query: 728 LVWSDSSHNVRSPITVYIVNKA 750
L+W+D H+VRSPITVY+V+KA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742
BLAST of Clc07G03720 vs. ExPASy Swiss-Prot
Match:
Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)
HSP 1 Score: 920.2 bits (2377), Expect = 1.5e-266
Identity = 466/736 (63.32%), Postives = 563/736 (76.49%), Query Frame = 0
Query: 8 MSSSLIFKLVVLLGLISSLFASSFNSNNDDGRKIYIVYMGNKPKDAASTSSHHMRMLEEV 67
MSSSLIFKL S+ AS +S +DDG+ IYIVYMG K +D S HH MLE+V
Sbjct: 1 MSSSLIFKLFFFSLFFSNRLASRLDS-DDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQV 60
Query: 68 IGSNFAPEALLHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTTRSWDFMG 127
+GS FAPE++LH+YKRSFNGF VKLTEEEA KI++ EGVVSVF N +HTTRSWDF+G
Sbjct: 61 VGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLG 120
Query: 128 FTKDVPRVNQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNFTCNKKII 187
F VPR +QVES+IVVGVLDTGIWPESPSF D G+ PPP KWKGTC+TS NF CN+KII
Sbjct: 121 FPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKII 180
Query: 188 GARAYRSDNFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 247
GAR+Y P D+ PRD+ GHGTHTAST AGGLV+QA+LYGL LGTARGGVP ARI
Sbjct: 181 GARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARI 240
Query: 248 AVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAFHSMKHGI 307
A YK+CW+DGCSD DILAA+DDAIADGVDIISLSVGG+ PR+YF D+IAIG+FH+++ GI
Sbjct: 241 AAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGI 300
Query: 308 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTINTFDLLEK 367
LTSNSAGN GP++FT + SPW LSVAAST+DRK V+QVQ+GN +QG +INTFD +
Sbjct: 301 LTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQ 360
Query: 368 QYPLIYAGDAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIYFSNAAGV 427
YPL+ D PN GF S SRFC+ SV+ NL++GKI++C+++ P F + A
Sbjct: 361 YYPLVSGRDIPNT--GFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAG 420
Query: 428 VMNDAGVKDRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDTSAPLIVSF 487
V+ + +D A+SYPLPSS L+ D Y+ S P ATI KS + + SAP++VSF
Sbjct: 421 VLMTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSF 480
Query: 488 SSRGPNPETLDILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISGTSMSCPHAT 547
SSRGPN T D++KPD++ PGVEILAAWP +APV G+ R T++NIISGTSMSCPH T
Sbjct: 481 SSRGPNRATKDVIKPDISGPGVEILAAWPSVAPV-GGI--RRNTLFNIISGTSMSCPHIT 540
Query: 548 AAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNIQAEFAYGAGHINPLQAINPGLVYDA 607
A YVKT++PTWSPAAIKSALMTTA P+NA N QAEFAYG+GH+NPL+A+ PGLVYDA
Sbjct: 541 GIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDA 600
Query: 608 NENDYVKFLCGQNYTTAMVQRLSNDNSICNSANFGRVWDLNYPSFALSSTPSEFINQIFT 667
NE+DYVKFLCGQ Y T V+R++ D S C S N GRVWDLNYPSF LS +PS+ NQ F
Sbjct: 601 NESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFN 660
Query: 668 RTLTNVESKASTYTSTVLGAPQGLIITVNPQVLSFDGIGQTKSFTLTIRGTISQSIVSAS 727
RTLT+V +ASTY + ++ APQGL I+VNP VLSF+G+G KSFTLT+RG+I +VSAS
Sbjct: 661 RTLTSVAPQASTYRA-MISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSAS 720
Query: 728 LVWSDSSHNVRSPITV 744
LVWSD H VRSPIT+
Sbjct: 721 LVWSDGVHYVRSPITI 727
BLAST of Clc07G03720 vs. ExPASy Swiss-Prot
Match:
Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)
HSP 1 Score: 637.5 bits (1643), Expect = 1.9e-181
Identity = 342/739 (46.28%), Postives = 472/739 (63.87%), Query Frame = 0
Query: 15 KLVVLLGLISSLFASSFNSNNDDGRK---IYIVYMGNKPKDAASTSSHHMRMLEEVIGSN 74
KL L LI F + + + +D R+ +YIVYMG P+ S SHH+ +L++++G+
Sbjct: 3 KLSTPLYLICLAFIFTRDVSANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVGTI 62
Query: 75 FAPEALLHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTTRSWDFMGFTKD 134
A L+ SYKRSFNGF L++ E+ K+ + VVSVFP+ + TTRSWDF+GF +
Sbjct: 63 AASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEK 122
Query: 135 VPRVNQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNFTCNKKIIGARA 194
R + ESD++VGV+D+GIWPES SF D G+GPPP KWKG+C+ F CN K+IGAR
Sbjct: 123 ARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARF 182
Query: 195 YRSDNFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYK 254
Y + S RD EGHGTHTAST AG V AS YGLA GTARGGVPSARIA YK
Sbjct: 183 YN-------KFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYK 242
Query: 255 ICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAFHSMKHGILTSN 314
+C+ + C+D DILAAFDDAIADGVD+IS+S+ N S+AIG+FH+M GI+T+
Sbjct: 243 VCF-NRCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAG 302
Query: 315 SAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTINTFDLLEKQYPL 374
SAGN+GPD ++ N SPW ++VAAS DR+ + +V LGN G ++NTF+L ++P+
Sbjct: 303 SAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPI 362
Query: 375 IYAGDAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIYFSNAAGVVMND 434
+Y N++ + + + +CS+ VD LV+GKI+LCD L Y + A GV++ +
Sbjct: 363 VY---GQNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQN 422
Query: 435 AGVKDRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDTSAPLIVSFSSRG 494
+ D A P P+S L D IK+Y+ S P A IL+++ + D AP + SFSSRG
Sbjct: 423 TLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRG 482
Query: 495 PNPETLDILKPDLTAPGVEILAAWPPIAPVSS--GVIDSRKTMYNIISGTSMSCPHATAA 554
P+ ++LKPD++APG+EILAA+ P+A SS D R Y+++SGTSM+CPH
Sbjct: 483 PSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGV 542
Query: 555 AVYVKTFHPTWSPAAIKSALMTTAIPLNAELNIQAEFAYGAGHINPLQAINPGLVYDANE 614
A YVK+FHP WSP+AIKSA+MTTA P+N + N + EFAYG+G INP +A +PGLVY+
Sbjct: 543 AAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVET 602
Query: 615 NDYVKFLCGQNYTTAMVQRLSNDNSICNSANFGRVWDLNYPSFALSSTPSEFINQIFTRT 674
DY+K LC + + + + S N C+ V DLNYP+ + + N F RT
Sbjct: 603 EDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--EVKDLNYPTMTTFVSSLDPFNVTFKRT 662
Query: 675 LTNVESKASTYTSTVLGAPQGLIITVNPQVLSFDGIGQTKSFTLTIRGTISQ--SIVSAS 734
+TNV STY ++V+ L I++ P++L F + + KSF +TI G + S VS+S
Sbjct: 663 VTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSS 722
Query: 735 LVWSDSSHNVRSPITVYIV 747
+VWSD SH+VRSPI Y +
Sbjct: 723 VVWSDGSHSVRSPIVAYSI 727
BLAST of Clc07G03720 vs. ExPASy Swiss-Prot
Match:
Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)
HSP 1 Score: 624.0 bits (1608), Expect = 2.2e-177
Identity = 335/737 (45.45%), Postives = 473/737 (64.18%), Query Frame = 0
Query: 22 LISSLFASSFNSNNDDGRKIYIVYMGNKP-KDAASTSSHHMRMLEEVIGSNFAPEALLHS 81
L+ SL + S + ++ +++YIVY+G+ P ++ + S HM +L+E+ G + L+ S
Sbjct: 15 LVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIENRLVRS 74
Query: 82 YKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTTRSWDFMGFTKDV--PRVNQV 141
YK+SFNGF +LTE E +++ E VVSVFP+ K + TT SW+FMG + + R +
Sbjct: 75 YKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSI 134
Query: 142 ESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNFTCNKKIIGARAYRSDNFF 201
ESD ++GV+D+GI+PES SFSD G+GPPP KWKGTC NFTCN K+IGAR Y + +
Sbjct: 135 ESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKS-- 194
Query: 202 PPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSDGC 261
+ Q+ RD GHGTHTAS AG V ++ YGL GTARGGVP+ARIAVYK+C ++GC
Sbjct: 195 --KANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGC 254
Query: 262 SDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAFHSMKHGILTSNSAGNDGP 321
+++AFDDAIADGVD+IS+S+ + D IAIGAFH+M G+LT N+AGN+GP
Sbjct: 255 DGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGP 314
Query: 322 DYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTINTFDLLEKQYPLIYAGDAP 381
T+ + +PW SVAAS +R +++V LG+ I G ++NT+D+ YPL+Y A
Sbjct: 315 KISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAA 374
Query: 382 NITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIYFSNAAGVVMNDAGVKDRA 441
T + +R C +DG LV+GKI+LCDS +V N DRA
Sbjct: 375 LST--CSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPE--PDRA 434
Query: 442 NSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDTSAPLIVSFSSRGPNPETLD 501
P S+L D + +YM S P AT+LKS+ +++ APL+ SFSSRGP+ D
Sbjct: 435 FIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSD 494
Query: 502 ILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISGTSMSCPHATAAAVYVKTFHP 561
ILKPD+TAPGVEILAA+ P + + D+R+ Y+++SGTSM+CPH A YVKTFHP
Sbjct: 495 ILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHP 554
Query: 562 TWSPAAIKSALMTTAIPLNAELN--IQAEFAYGAGHINPLQAINPGLVYDANENDYVKFL 621
WSP+ I+SA+MTTA P+NA + + EFAYG+GH++P+ AINPGLVY+ + D++ FL
Sbjct: 555 QWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFL 614
Query: 622 CGQNYTTAMVQRLSNDNSICN---SANFGRVWDLNYPSFALSSTPSEFINQIFTRTLTNV 681
CG NYT+ ++ +S DNS C S R +LNYP+ + + ++ N F RT+TNV
Sbjct: 615 CGLNYTSDHLRIISGDNSTCTKEISKTLPR--NLNYPTMSAKVSGTKPFNITFQRTVTNV 674
Query: 682 ESKASTYTSTVLGAP-QGLIITVNPQVLSFDGIGQTKSFTLTIRGTI--SQSIVSASLVW 741
+ STY + V+ P L I V+P+VLS + + +SF +T+ ++ VSA+L+W
Sbjct: 675 GMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPVSANLIW 734
Query: 742 SDSSHNVRSPITVYIVN 748
SD +HNVRSPI VY ++
Sbjct: 735 SDGTHNVRSPIIVYAMS 741
BLAST of Clc07G03720 vs. ExPASy Swiss-Prot
Match:
Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)
HSP 1 Score: 622.5 bits (1604), Expect = 6.3e-177
Identity = 344/736 (46.74%), Postives = 468/736 (63.59%), Query Frame = 0
Query: 19 LLGLISSLFASSFNSNNDDGRKIYIVYMGNKPKDAAST-SSHHMRMLEEVIGSNFAPEAL 78
LL + LF SS ++ DD +++YIVYMG+ A T +S HM +L+EV G + L
Sbjct: 10 LLSCLLVLFLSSVSAVTDD-KQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESSIEGRL 69
Query: 79 LHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTTRSWDFMGFTKDV--PRV 138
+ SYKRSFNGF +LTE E +++ GVVSVFPN K + TT SWDFMG + + R
Sbjct: 70 VRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRN 129
Query: 139 NQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNFTCNKKIIGARAYRSD 198
VESD ++GV+D+GI PES SFSD G+GPPP KWKG C NFTCN K+IGAR Y S
Sbjct: 130 PTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTS- 189
Query: 199 NFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWS 258
+ RD +GHGTHTAST AG V AS +G+ GT RGGVP++R+A YK+C
Sbjct: 190 --------EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTP 249
Query: 259 DGCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAFHSMKHGILTSNSAGN 318
GCS +L+AFDDAIADGVD+I++S+G + ND IAIGAFH+M G+LT NSAGN
Sbjct: 250 TGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGN 309
Query: 319 DGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTINTFDLLEKQYPLIYAG 378
GP ++ +PW L+VAAST +R V++V LGN G ++N +++ K YPL+Y
Sbjct: 310 SGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGK 369
Query: 379 DAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIYFSNAAGVVMNDAGVK 438
A + + C + VD + V+GKIL+C + A G++
Sbjct: 370 SA--ASSACDAESAGLCELSCVDKSRVKGKILVCGGP-GGLKIVESVGAVGLIYR-TPKP 429
Query: 439 DRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDTSAPLIVSFSSRGPNPE 498
D A +PLP++ L T D + +Y+ S P A +LK++A+ + ++P+I SFSSRGPN
Sbjct: 430 DVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTI 489
Query: 499 TLDILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISGTSMSCPHATAAAVYVKT 558
+DILKPD+TAPGVEILAA+ P S D+R Y+++SGTSMSCPH A YVKT
Sbjct: 490 AVDILKPDITAPGVEILAAYSPAGEPSQD--DTRHVKYSVLSGTSMSCPHVAGVAAYVKT 549
Query: 559 FHPTWSPAAIKSALMTTAIPLNAELN--IQAEFAYGAGHINPLQAINPGLVYDANENDYV 618
F+P WSP+ I+SA+MTTA P+NA EFAYG+GH++P+ A NPGLVY+ +++D++
Sbjct: 550 FNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHI 609
Query: 619 KFLCGQNYTTAMVQRLSNDNSICNSANFGRVWDLNYPSFA--LSSTPSEFINQIFTRTLT 678
FLCG NYT+ +++ +S + C+ A +LNYPS + LS + + F F RTLT
Sbjct: 610 AFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTF-TVTFNRTLT 669
Query: 679 NVESKASTYTSTVL-GAPQGLIITVNPQVLSFDGIGQTKSFTLTIRGTISQSIV--SASL 738
NV + STYTS V+ G L + + P VLSF + + +SFT+T+ G+ S V SA+L
Sbjct: 670 NVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANL 728
Query: 739 VWSDSSHNVRSPITVY 745
+WSD +HNVRSPI VY
Sbjct: 730 IWSDGTHNVRSPIVVY 728
BLAST of Clc07G03720 vs. ExPASy Swiss-Prot
Match:
Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)
HSP 1 Score: 618.2 bits (1593), Expect = 1.2e-175
Identity = 345/754 (45.76%), Postives = 476/754 (63.13%), Query Frame = 0
Query: 6 NTMSSSLIFK--LVVLLGLISSLFASSFNSNNDDGRKIYIVYMGNKPKDA-ASTSSHHMR 65
N +S+ ++ LV+LL +S++ D+ ++YIVYMG+ A +S HM
Sbjct: 3 NLAASTCLYSWLLVLLLSSVSAII--------DEDTQVYIVYMGSLSSRADYIPTSDHMS 62
Query: 66 MLEEVIGSNFAPEALLHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTTRS 125
+L++V G + L+ SYKRSFNGF +LTE E I+ EGVVSVFPN +HTT S
Sbjct: 63 ILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTS 122
Query: 126 WDFMGFT--KDVPRVNQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNF 185
WDFMG K+ R +ESD ++GV+DTGIWPES SFSD G+GPPP KWKG C NF
Sbjct: 123 WDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNF 182
Query: 186 TCNKKIIGARAYRSDNFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARG 245
TCN K+IGAR Y S + RD+ GHGTHTAST AG V S +G+ GT RG
Sbjct: 183 TCNNKLIGARDYTS---------EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRG 242
Query: 246 GVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAF 305
GVP++RIA YK+C GCS +L++FDDAIADGVD+I++S+G P + +D IAIGAF
Sbjct: 243 GVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAF 302
Query: 306 HSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTIN 365
H+M GILT +SAGN GP T+ + +PW +VAAST +R +++V LGN G ++N
Sbjct: 303 HAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVN 362
Query: 366 TFDLLEKQYPLIYAGDAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIY 425
FD+ K+YPL+Y A + + C+ ++ + V+GKIL+C PS +
Sbjct: 363 AFDMKGKKYPLVYGKSA--ASSACDAKTAALCAPACLNKSRVKGKILVCG---GPSGYKI 422
Query: 426 FSNAAGVVMNDAGVK-DRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDT 485
+ + + D + D A ++ LP+S L+ D + +Y+ S P A +LK++ + +
Sbjct: 423 AKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNR 482
Query: 486 SAPLIVSFSSRGPNPETLDILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISGT 545
++P+I SFSSRGPN +DILKPD+TAPGVEILAA+ P S D+R+ Y++ SGT
Sbjct: 483 TSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGT 542
Query: 546 SMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELN--IQAEFAYGAGHINPLQ 605
SM+CPH A YVKTF+P WSP+ I+SA+MTTA P+ A+ EFAYGAGH++P+
Sbjct: 543 SMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMA 602
Query: 606 AINPGLVYDANENDYVKFLCGQNYTTAMVQRLSNDNSICNSANFGRVWDLNYPSFA--LS 665
A+NPGLVY+ ++ D++ FLCG NYT+ ++ +S D C+ N +LNYPS + LS
Sbjct: 603 ALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLS 662
Query: 666 STPSEFINQIFTRTLTNVESKASTYTSTVL-GAPQGLIITVNPQVLSFDGIGQTKSFTLT 725
T S F + F RTLTNV + STY S V+ G L I V P VL F + + +SF++T
Sbjct: 663 GTDSTF-SVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVT 722
Query: 726 IRGTISQSIV--SASLVWSDSSHNVRSPITVYIV 747
+ G+ S V SA+L+WSD +HNVRSPI VYI+
Sbjct: 723 VTGSDVDSEVPSSANLIWSDGTHNVRSPIVVYIM 731
BLAST of Clc07G03720 vs. ExPASy TrEMBL
Match:
A0A0A0KLY4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G187860 PE=3 SV=1)
HSP 1 Score: 1271.1 bits (3288), Expect = 0.0e+00
Identity = 637/742 (85.85%), Postives = 681/742 (91.78%), Query Frame = 0
Query: 8 MSSSLIFK--LVVLLGLISSLFASSFNSNNDDGRKIYIVYMGNKPKDAASTSSHHMRMLE 67
MSSSLIFK LV++LGL+SSL AS F+S NDD RKIYIVYMGNKP+D AST SHHMRML
Sbjct: 1 MSSSLIFKLALVLVLGLVSSLLASGFDSKNDD-RKIYIVYMGNKPQDTASTPSHHMRMLR 60
Query: 68 EVIGSNFAPEALLHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTTRSWDF 127
EV GSNFAPE+LLHSYKRSFNGFVVKLTEEEAH+ISAKEGVVSVFP+GKKH+HTTRSWDF
Sbjct: 61 EVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDF 120
Query: 128 MGFTKDVPRVNQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNFTCNKK 187
+GFTKDVPRVNQVESDIVVGVLD+GIWPE+PSFSD GYGP PAKWKG CQ TNFTCNKK
Sbjct: 121 IGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKK 180
Query: 188 IIGARAYRSDNFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSA 247
IIGARAYRSDN FP +DI SPRDS GHGTHTASTVAGGLV+QASLYGLALGTARGGVPSA
Sbjct: 181 IIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSA 240
Query: 248 RIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAFHSMKH 307
RIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSE RYYFNDSIAIGAFHSMKH
Sbjct: 241 RIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKH 300
Query: 308 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTINTFDLL 367
GILTSNSAGNDGPDYFTIRNFSPWSLSVAAST DRKLVS+V++GN N+YQGYTINTFD L
Sbjct: 301 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPL 360
Query: 368 EKQYPLIYAGDAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIYFSNAA 427
KQYPLIYAGDAPN+ GGFTGSISRFCS SVD NLV GKILLCDS LAPS F+YFS+A
Sbjct: 361 GKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAV 420
Query: 428 GVVMNDAGVKDRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDTSAPLIV 487
GVVMND GVK +NSYPLPSSYLET DG+ IKTYM SNGVPTATI KSDAVND+SAP IV
Sbjct: 421 GVVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSSAPFIV 480
Query: 488 SFSSRGPNPETLDILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISGTSMSCPH 547
SFSSRGPNPETLDILKPDLTAPGVEILAAW PIAPVSSGVIDSR T+YNIISGTSMSCPH
Sbjct: 481 SFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPH 540
Query: 548 ATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNIQAEFAYGAGHINPLQAINPGLVY 607
TAAAVYVKTFHPTWSPAAIKSALMTTA PL E+N++AEFAYGAG INPL+AI+PGLVY
Sbjct: 541 VTAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVY 600
Query: 608 DANENDYVKFLCGQNYTTAMVQRLSNDNSICNSANFGRVWDLNYPSFALSSTPSEFINQI 667
DANE DYVKFLCGQ YT+ MVQ LSNDN+ICNSAN GRVWDLNYPSFALSSTPS+ INQ
Sbjct: 601 DANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQF 660
Query: 668 FTRTLTNVESKASTYTSTVLGAPQGLIITVNPQVLSFDGIGQTKSFTLTIRGTIS-QSIV 727
FTRTLT+V+S ASTYTST+LGAPQGL ITVNP+VLSF GIG+ K+FTLTI+GTI +IV
Sbjct: 661 FTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSGIGEKKTFTLTIQGTIDPTTIV 720
Query: 728 SASLVWSDSSHNVRSPITVYIV 747
SASLVWSDSSH+VRSPIT+Y+V
Sbjct: 721 SASLVWSDSSHDVRSPITIYVV 741
BLAST of Clc07G03720 vs. ExPASy TrEMBL
Match:
A0A1S3CF99 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500258 PE=3 SV=1)
HSP 1 Score: 1266.9 bits (3277), Expect = 0.0e+00
Identity = 634/750 (84.53%), Postives = 683/750 (91.07%), Query Frame = 0
Query: 1 MAMTRNTMSSSLIFK--LVVLLGLISSLFASSFNSNNDDGRKIYIVYMGNKPKDAASTSS 60
+ MTRN MSSSLIFK LV++LGL+ SL AS +S N D RKIYIVYMGNKP+D AST S
Sbjct: 7 LVMTRNIMSSSLIFKLALVLVLGLVFSLLASGLDSENVD-RKIYIVYMGNKPQDTASTPS 66
Query: 61 HHMRMLEEVIGSNFAPEALLHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVH 120
HHMRML EV GSNFAPE+LLHSYKRSFNGFVVKLTEEEAH+ISAKEGVVSVFP+GKKH+H
Sbjct: 67 HHMRMLREVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLH 126
Query: 121 TTRSWDFMGFTKDVPRVNQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTST 180
TTRSWDF+GFTKDVPRVNQVESDIVVGVLD+GIWPE+PSFSDVGYGP PAKWKG+CQTS
Sbjct: 127 TTRSWDFLGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDVGYGPIPAKWKGSCQTSA 186
Query: 181 NFTCNKKIIGARAYRSDNFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTA 240
NFTCNKKIIGARAYRSDN FP DI SPRDS GHGTHTASTVAGGLV+QASLYGLALGTA
Sbjct: 187 NFTCNKKIIGARAYRSDNVFPTADIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTA 246
Query: 241 RGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIG 300
RGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSE RYYFNDSIAIG
Sbjct: 247 RGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIG 306
Query: 301 AFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYT 360
AFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAAST DRKLVS+V++GN N+YQGYT
Sbjct: 307 AFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYT 366
Query: 361 INTFDLLEKQYPLIYAGDAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTF 420
INTFD L KQYPLIYAGDAPNI GGFTGSISRFCS SVD NLV GKILLC S +APS F
Sbjct: 367 INTFDPLGKQYPLIYAGDAPNIDGGFTGSISRFCSEGSVDANLVSGKILLCVSIVAPSAF 426
Query: 421 IYFSNAAGVVMNDAGVKDRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVND 480
+YFS A GVVMND GVK +NSYPLPSSYL+T DG+ IKTYM SNGVPTATI KSDAVND
Sbjct: 427 VYFSEAVGVVMNDDGVKYPSNSYPLPSSYLQTVDGDAIKTYMASNGVPTATIFKSDAVND 486
Query: 481 TSAPLIVSFSSRGPNPETLDILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISG 540
+ AP +VSFSSRGPNPETLDILKPDLTAPG EILA W PIAPVSSGVIDSR TMYNIISG
Sbjct: 487 SYAPFVVSFSSRGPNPETLDILKPDLTAPGAEILAGWSPIAPVSSGVIDSRTTMYNIISG 546
Query: 541 TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNIQAEFAYGAGHINPLQA 600
TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTA PL ++N++AEFAYGAG INPL+A
Sbjct: 547 TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPDINVEAEFAYGAGQINPLKA 606
Query: 601 INPGLVYDANENDYVKFLCGQNYTTAMVQRLSNDNSICNSANFGRVWDLNYPSFALSSTP 660
INPGLVYDANE+DYVKFLCGQ YT+AMVQ LSNDN++CNSAN GRVWDLNYPSFALSSTP
Sbjct: 607 INPGLVYDANESDYVKFLCGQGYTSAMVQSLSNDNTLCNSANIGRVWDLNYPSFALSSTP 666
Query: 661 SEFINQIFTRTLTNVESKASTYTSTVLGAPQGLIITVNPQVLSFDGIGQTKSFTLTIRGT 720
S+ INQ FTRTLT+V+S ASTYTST+LGAPQGL ITVNP+VLSF G+G+ +FTLTI+G+
Sbjct: 667 SQSINQFFTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSGMGEKNTFTLTIQGS 726
Query: 721 IS-QSIVSASLVWSDSSHNVRSPITVYIVN 748
I SIVSASLVWSDSSH+VRSPIT+Y+V+
Sbjct: 727 IDPTSIVSASLVWSDSSHDVRSPITIYVVS 755
BLAST of Clc07G03720 vs. ExPASy TrEMBL
Match:
A0A6J1FQ02 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447181 PE=3 SV=1)
HSP 1 Score: 1245.3 bits (3221), Expect = 0.0e+00
Identity = 622/749 (83.04%), Postives = 677/749 (90.39%), Query Frame = 0
Query: 1 MAMTRNTMSSSLIFKLVVLLGLISSLFASSFNSNNDDGRKIYIVYMGNKPKDAASTSSHH 60
M M N SSSLIFKLVV L L+ L ASSF+S N DGRK+YIVY+GNK +D AST SHH
Sbjct: 39 MIMAGNNASSSLIFKLVV-LNLVCCLLASSFDSGN-DGRKVYIVYLGNKREDTASTPSHH 98
Query: 61 MRMLEEVIGSNFAPEALLHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTT 120
RMLEE IGS FAPEALLHSYKRSFNGFVV+LTEEEA KISAKEGVVSVFPN KKH HTT
Sbjct: 99 TRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKEGVVSVFPNEKKHPHTT 158
Query: 121 RSWDFMGFTKDVPRVNQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNF 180
RSWDFMGFT +VPRV QVESDIVVGVLDTGIWPESPSF+DVGYGPPPAKWKG CQTSTNF
Sbjct: 159 RSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFNDVGYGPPPAKWKGACQTSTNF 218
Query: 181 TCNKKIIGARAYRSDNFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARG 240
CNKKIIGARAYRSDN FPP+D +SPRDSEGHGTHTASTVAGGLV+QASL+GLALGTARG
Sbjct: 219 RCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARG 278
Query: 241 GVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAF 300
GVPSARIAVYKICWSDGC DADILAAFDDAIADGVDIISLSVGG+EP++YFNDSIAIGAF
Sbjct: 279 GVPSARIAVYKICWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAF 338
Query: 301 HSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTIN 360
HSMK+GILTSNSAGNDGPD+ TIRNFSPWSLSVAAS+IDRKLVS+VQLGN NI+QG+TIN
Sbjct: 339 HSMKNGILTSNSAGNDGPDFLTIRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTIN 398
Query: 361 TFDLLEKQYPLIYAGDAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIY 420
TFDL +KQ+PLIYAG+APNI+GGFTG+ SRFCS NSVD NLV+GKIL+CDS ++PSTF
Sbjct: 399 TFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSIVSPSTFAS 458
Query: 421 FSNAAGVVMNDAGVKDRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDTS 480
FS+A GVVMNDAGVKD A SYPLPSSYL T GN+IKTYM SN PTATI KS+AVNDTS
Sbjct: 459 FSSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTS 518
Query: 481 APLIVSFSSRGPNPETLDILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISGTS 540
AP++VSFSSRGPNPETLDILKPDLTAPGVEILAAWPP APVSSG+ DSR T+YNIISGTS
Sbjct: 519 APIVVSFSSRGPNPETLDILKPDLTAPGVEILAAWPPDAPVSSGIRDSRSTLYNIISGTS 578
Query: 541 MSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNIQAEFAYGAGHINPLQAIN 600
MSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLN LN QAEFAYGAGHI+PL+A N
Sbjct: 579 MSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATN 638
Query: 601 PGLVYDANENDYVKFLCGQNYTTAMVQRLSNDNSICNSANFGRVWDLNYPSFALSSTPSE 660
PGLVYDANE DYV FLCGQ Y+TAMV+RL+ D S+C +AN GRVWDLNYPSFALS+TPSE
Sbjct: 639 PGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSE 698
Query: 661 FINQIFTRTLTNVESKASTYTSTVLGAPQGLIITVNPQVLSFDGIGQTKSFTLTIRGTIS 720
INQ FTRTLTNV+++ASTYTS +LGAP+GL ITV+P LSF+GIG KSFTLTI GTI
Sbjct: 699 SINQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPALSFNGIGDKKSFTLTIDGTII 758
Query: 721 QSIVSASLVWSDSSHNVRSPITVYIVNKA 750
Q+IVSAS+VWSD SHNVRSPIT+YIVNKA
Sbjct: 759 QTIVSASVVWSDGSHNVRSPITIYIVNKA 785
BLAST of Clc07G03720 vs. ExPASy TrEMBL
Match:
A0A6J1IQ27 (cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111478984 PE=3 SV=1)
HSP 1 Score: 1245.0 bits (3220), Expect = 0.0e+00
Identity = 614/742 (82.75%), Postives = 674/742 (90.84%), Query Frame = 0
Query: 8 MSSSLIFKLVVLLGLISSLFASSFNSNNDDGRKIYIVYMGNKPKDAASTSSHHMRMLEEV 67
MSSSLIFKLV+ + SL ASS +S+++DGRK+YIVY+GNKP+D+AST SHHMRMLEEV
Sbjct: 1 MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
Query: 68 IGSNFAPEALLHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTTRSWDFMG 127
+GS FAP+ALLHSYKRSFNGFVVKLTEEEA KISAKEGVVSVFPNGKKH+HTTRSWDFMG
Sbjct: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
Query: 128 FTKDVPRVNQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNFTCNKKII 187
FTK V RV QVES+IVVGVLD+GIWPESPSFSDVGYGPPPAKWKG CQTS NF CN+KII
Sbjct: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
Query: 188 GARAYRSDNFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 247
GARAYRSDN FPP+DI+SPRDS+GHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
Query: 248 AVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAFHSMKHGI 307
AVYKICWSDGC DADILAAFDDAIADGVDIISLSVGG++P+YYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
Query: 308 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTINTFDLLEK 367
LTSNSAGNDGPDYFTIRNFSPWSLSVAAS+IDRKLVS+VQLGN N+YQGYTINTFDL K
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
Query: 368 QYPLIYAGDAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIYFSNAAGV 427
QYPLIYAG+APN++GGFTGS SRFCS NSVD NLVRGKILLCDS L+PSTF F+ A GV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
Query: 428 VMNDAGVKDRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDTSAPLIVSF 487
VMNDAGVKD + SYPLPSSYL GN+IKTYM SN PTATI KS+AVNDTSAPLIVSF
Sbjct: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
Query: 488 SSRGPNPETLDILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISGTSMSCPHAT 547
SSRGPNPET DILKPDLTAPGVEILAAW PIA VSSGV DSR T+YNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
Query: 548 AAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNIQAEFAYGAGHINPLQAINPGLVYDA 607
AAAVYVKTFHP+WSPAAIKSALMTTAIPLN +LN QAEFAYGAGHINP++A+NPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
Query: 608 NENDYVKFLCGQNYTTAMVQRLSNDNSICNSANFGRVWDLNYPSFALSSTPSEFINQIFT 667
E+DYV+FLCGQ YTTAMV+RLS D+S+C AN GRVWDLNYPSFALSSTPSE INQ F
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
Query: 668 RTLTNVESKASTYTSTVLGAPQGLIITVNPQVLSFDGIGQTKSFTLTIRGTISQSIVSAS 727
RT+TNV SK +TY + VLGAP+GL I+VNP LSF+ IGQ KSFT+T+RG ++Q IVSA+
Sbjct: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
Query: 728 LVWSDSSHNVRSPITVYIVNKA 750
L+W+D H+VRSPITVY+V+KA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742
BLAST of Clc07G03720 vs. ExPASy TrEMBL
Match:
A0A6J1FV97 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447169 PE=3 SV=1)
HSP 1 Score: 1242.3 bits (3213), Expect = 0.0e+00
Identity = 613/742 (82.61%), Postives = 674/742 (90.84%), Query Frame = 0
Query: 8 MSSSLIFKLVVLLGLISSLFASSFNSNNDDGRKIYIVYMGNKPKDAASTSSHHMRMLEEV 67
MSSSLIFKLV+ + SL AS +S+++DGRK+YIVY+GNKP+D+AST SHHMRMLEEV
Sbjct: 1 MSSSLIFKLVIFISFSGSLLASGLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
Query: 68 IGSNFAPEALLHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTTRSWDFMG 127
+GS FAP+ALLHSYKRSFNGFVVKLTEEEA KISAKEGVVSVFPNGKKH+HTTRSWDFMG
Sbjct: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
Query: 128 FTKDVPRVNQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNFTCNKKII 187
FTK+V RV QVES+IVVGVLD+GIWPESPSFSDVGYGPPPAKWKG CQTS NF CN+KII
Sbjct: 121 FTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
Query: 188 GARAYRSDNFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 247
GARAYRSDN FPP+DI+SPRDS+GHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
Query: 248 AVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAFHSMKHGI 307
AVYKICWSDGC DADILAAFDDAIADGVDIISLSVGG++P+YYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
Query: 308 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTINTFDLLEK 367
LTSNSAGNDGPDYFTIRNFSPWSLSVAAS+IDRKLVS+VQLGN N+YQGYTINTFDL K
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
Query: 368 QYPLIYAGDAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIYFSNAAGV 427
QYPLIYAG+APN++GGFTGS SRFCS NSVD NLVRGKILLCDS L+PSTF F+ A GV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
Query: 428 VMNDAGVKDRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDTSAPLIVSF 487
VMNDAGVKD A SYPLPSSYL GN+IKTYM S+ PTATI KS+AVNDTSAPLIVSF
Sbjct: 421 VMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSSKFPTATIFKSNAVNDTSAPLIVSF 480
Query: 488 SSRGPNPETLDILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISGTSMSCPHAT 547
SSRGPNPET DILKPDLTAPGVEILAAW PIA VSSGV DSR T+YNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
Query: 548 AAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNIQAEFAYGAGHINPLQAINPGLVYDA 607
AAAVYVKTFHP+WSPAAIKSALMTTAIPLN +LN QAEFAYGAGHINP++A+NPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
Query: 608 NENDYVKFLCGQNYTTAMVQRLSNDNSICNSANFGRVWDLNYPSFALSSTPSEFINQIFT 667
E+DYV+FLCGQ YTTAMV+RLS D+S+C AN GRVWDLNYPSFALSSTPSE INQ F
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTHANSGRVWDLNYPSFALSSTPSESINQFFR 660
Query: 668 RTLTNVESKASTYTSTVLGAPQGLIITVNPQVLSFDGIGQTKSFTLTIRGTISQSIVSAS 727
RT+TNV SK +TY + VLG P+GL I+VNP VLSF+ IGQ KSFT+T+RG ++Q IVSA+
Sbjct: 661 RTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
Query: 728 LVWSDSSHNVRSPITVYIVNKA 750
L+W+D H+VRSPITVY+V+KA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742
BLAST of Clc07G03720 vs. TAIR 10
Match:
AT5G59190.1 (subtilase family protein )
HSP 1 Score: 627.5 bits (1617), Expect = 1.4e-179
Identity = 330/705 (46.81%), Postives = 454/705 (64.40%), Query Frame = 0
Query: 46 MGNKPKDAASTSSHHMRMLEEVIGSNFAPEALLHSYKRSFNGFVVKLTEEEAHKISAKEG 105
MG P+ S SHH+ +L++++G+ A L+ SYKRSFNGF L++ E+ K+ +
Sbjct: 1 MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60
Query: 106 VVSVFPNGKKHVHTTRSWDFMGFTKDVPRVNQVESDIVVGVLDTGIWPESPSFSDVGYGP 165
VVSVFP+ + TTRSWDF+GF + R + ESD++VGV+D+GIWPES SF D G+GP
Sbjct: 61 VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120
Query: 166 PPAKWKGTCQTSTNFTCNKKIIGARAYRSDNFFPPQDIQSPRDSEGHGTHTASTVAGGLV 225
PP KWKG+C+ F CN K+IGAR Y + S RD EGHGTHTAST AG V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYN-------KFADSARDEEGHGTHTASTAAGNAV 180
Query: 226 NQASLYGLALGTARGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGS 285
AS YGLA GTARGGVPSARIA YK+C+ + C+D DILAAFDDAIADGVD+IS+S+
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240
Query: 286 EPRYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSQ 345
N S+AIG+FH+M GI+T+ SAGN+GPD ++ N SPW ++VAAS DR+ + +
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300
Query: 346 VQLGNMNIYQGYTINTFDLLEKQYPLIYAGDAPNITGGFTGSISRFCSANSVDGNLVRGK 405
V LGN G ++NTF+L ++P++Y N++ + + + +CS+ VD LV+GK
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAGYCSSGCVDSELVKGK 360
Query: 406 ILLCDSALAPSTFIYFSNAAGVVMNDAGVKDRANSYPLPSSYLETADGNDIKTYMTSNGV 465
I+LCD L Y + A GV++ + + D A P P+S L D IK+Y+ S
Sbjct: 361 IVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP 420
Query: 466 PTATILKSDAVNDTSAPLIVSFSSRGPNPETLDILKPDLTAPGVEILAAWPPIAPVSS-- 525
P A IL+++ + D AP + SFSSRGP+ ++LKPD++APG+EILAA+ P+A SS
Sbjct: 421 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 480
Query: 526 GVIDSRKTMYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNIQ 585
D R Y+++SGTSM+CPH A YVK+FHP WSP+AIKSA+MTTA P+N + N +
Sbjct: 481 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 540
Query: 586 AEFAYGAGHINPLQAINPGLVYDANENDYVKFLCGQNYTTAMVQRLSNDNSICNSANFGR 645
EFAYG+G INP +A +PGLVY+ DY+K LC + + + + S N C+
Sbjct: 541 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--E 600
Query: 646 VWDLNYPSFALSSTPSEFINQIFTRTLTNVESKASTYTSTVLGAPQGLIITVNPQVLSFD 705
V DLNYP+ + + N F RT+TNV STY ++V+ L I++ P++L F
Sbjct: 601 VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFG 660
Query: 706 GIGQTKSFTLTIRGTISQ--SIVSASLVWSDSSHNVRSPITVYIV 747
+ + KSF +TI G + S VS+S+VWSD SH+VRSPI Y +
Sbjct: 661 FLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 691
BLAST of Clc07G03720 vs. TAIR 10
Match:
AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 624.0 bits (1608), Expect = 1.5e-178
Identity = 335/737 (45.45%), Postives = 473/737 (64.18%), Query Frame = 0
Query: 22 LISSLFASSFNSNNDDGRKIYIVYMGNKP-KDAASTSSHHMRMLEEVIGSNFAPEALLHS 81
L+ SL + S + ++ +++YIVY+G+ P ++ + S HM +L+E+ G + L+ S
Sbjct: 15 LVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIENRLVRS 74
Query: 82 YKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTTRSWDFMGFTKDV--PRVNQV 141
YK+SFNGF +LTE E +++ E VVSVFP+ K + TT SW+FMG + + R +
Sbjct: 75 YKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSI 134
Query: 142 ESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNFTCNKKIIGARAYRSDNFF 201
ESD ++GV+D+GI+PES SFSD G+GPPP KWKGTC NFTCN K+IGAR Y + +
Sbjct: 135 ESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKS-- 194
Query: 202 PPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSDGC 261
+ Q+ RD GHGTHTAS AG V ++ YGL GTARGGVP+ARIAVYK+C ++GC
Sbjct: 195 --KANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGC 254
Query: 262 SDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAFHSMKHGILTSNSAGNDGP 321
+++AFDDAIADGVD+IS+S+ + D IAIGAFH+M G+LT N+AGN+GP
Sbjct: 255 DGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGP 314
Query: 322 DYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTINTFDLLEKQYPLIYAGDAP 381
T+ + +PW SVAAS +R +++V LG+ I G ++NT+D+ YPL+Y A
Sbjct: 315 KISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAA 374
Query: 382 NITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIYFSNAAGVVMNDAGVKDRA 441
T + +R C +DG LV+GKI+LCDS +V N DRA
Sbjct: 375 LST--CSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPE--PDRA 434
Query: 442 NSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDTSAPLIVSFSSRGPNPETLD 501
P S+L D + +YM S P AT+LKS+ +++ APL+ SFSSRGP+ D
Sbjct: 435 FIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSD 494
Query: 502 ILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISGTSMSCPHATAAAVYVKTFHP 561
ILKPD+TAPGVEILAA+ P + + D+R+ Y+++SGTSM+CPH A YVKTFHP
Sbjct: 495 ILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHP 554
Query: 562 TWSPAAIKSALMTTAIPLNAELN--IQAEFAYGAGHINPLQAINPGLVYDANENDYVKFL 621
WSP+ I+SA+MTTA P+NA + + EFAYG+GH++P+ AINPGLVY+ + D++ FL
Sbjct: 555 QWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFL 614
Query: 622 CGQNYTTAMVQRLSNDNSICN---SANFGRVWDLNYPSFALSSTPSEFINQIFTRTLTNV 681
CG NYT+ ++ +S DNS C S R +LNYP+ + + ++ N F RT+TNV
Sbjct: 615 CGLNYTSDHLRIISGDNSTCTKEISKTLPR--NLNYPTMSAKVSGTKPFNITFQRTVTNV 674
Query: 682 ESKASTYTSTVLGAP-QGLIITVNPQVLSFDGIGQTKSFTLTIRGTI--SQSIVSASLVW 741
+ STY + V+ P L I V+P+VLS + + +SF +T+ ++ VSA+L+W
Sbjct: 675 GMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPVSANLIW 734
Query: 742 SDSSHNVRSPITVYIVN 748
SD +HNVRSPI VY ++
Sbjct: 735 SDGTHNVRSPIIVYAMS 741
BLAST of Clc07G03720 vs. TAIR 10
Match:
AT5G59120.1 (subtilase 4.13 )
HSP 1 Score: 622.5 bits (1604), Expect = 4.5e-178
Identity = 344/736 (46.74%), Postives = 468/736 (63.59%), Query Frame = 0
Query: 19 LLGLISSLFASSFNSNNDDGRKIYIVYMGNKPKDAAST-SSHHMRMLEEVIGSNFAPEAL 78
LL + LF SS ++ DD +++YIVYMG+ A T +S HM +L+EV G + L
Sbjct: 10 LLSCLLVLFLSSVSAVTDD-KQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESSIEGRL 69
Query: 79 LHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTTRSWDFMGFTKDV--PRV 138
+ SYKRSFNGF +LTE E +++ GVVSVFPN K + TT SWDFMG + + R
Sbjct: 70 VRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRN 129
Query: 139 NQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNFTCNKKIIGARAYRSD 198
VESD ++GV+D+GI PES SFSD G+GPPP KWKG C NFTCN K+IGAR Y S
Sbjct: 130 PTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTS- 189
Query: 199 NFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWS 258
+ RD +GHGTHTAST AG V AS +G+ GT RGGVP++R+A YK+C
Sbjct: 190 --------EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTP 249
Query: 259 DGCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAFHSMKHGILTSNSAGN 318
GCS +L+AFDDAIADGVD+I++S+G + ND IAIGAFH+M G+LT NSAGN
Sbjct: 250 TGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGN 309
Query: 319 DGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTINTFDLLEKQYPLIYAG 378
GP ++ +PW L+VAAST +R V++V LGN G ++N +++ K YPL+Y
Sbjct: 310 SGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGK 369
Query: 379 DAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIYFSNAAGVVMNDAGVK 438
A + + C + VD + V+GKIL+C + A G++
Sbjct: 370 SA--ASSACDAESAGLCELSCVDKSRVKGKILVCGGP-GGLKIVESVGAVGLIYR-TPKP 429
Query: 439 DRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDTSAPLIVSFSSRGPNPE 498
D A +PLP++ L T D + +Y+ S P A +LK++A+ + ++P+I SFSSRGPN
Sbjct: 430 DVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTI 489
Query: 499 TLDILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISGTSMSCPHATAAAVYVKT 558
+DILKPD+TAPGVEILAA+ P S D+R Y+++SGTSMSCPH A YVKT
Sbjct: 490 AVDILKPDITAPGVEILAAYSPAGEPSQD--DTRHVKYSVLSGTSMSCPHVAGVAAYVKT 549
Query: 559 FHPTWSPAAIKSALMTTAIPLNAELN--IQAEFAYGAGHINPLQAINPGLVYDANENDYV 618
F+P WSP+ I+SA+MTTA P+NA EFAYG+GH++P+ A NPGLVY+ +++D++
Sbjct: 550 FNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHI 609
Query: 619 KFLCGQNYTTAMVQRLSNDNSICNSANFGRVWDLNYPSFA--LSSTPSEFINQIFTRTLT 678
FLCG NYT+ +++ +S + C+ A +LNYPS + LS + + F F RTLT
Sbjct: 610 AFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTF-TVTFNRTLT 669
Query: 679 NVESKASTYTSTVL-GAPQGLIITVNPQVLSFDGIGQTKSFTLTIRGTISQSIV--SASL 738
NV + STYTS V+ G L + + P VLSF + + +SFT+T+ G+ S V SA+L
Sbjct: 670 NVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANL 728
Query: 739 VWSDSSHNVRSPITVY 745
+WSD +HNVRSPI VY
Sbjct: 730 IWSDGTHNVRSPIVVY 728
BLAST of Clc07G03720 vs. TAIR 10
Match:
AT5G59090.1 (subtilase 4.12 )
HSP 1 Score: 618.2 bits (1593), Expect = 8.5e-177
Identity = 345/754 (45.76%), Postives = 476/754 (63.13%), Query Frame = 0
Query: 6 NTMSSSLIFK--LVVLLGLISSLFASSFNSNNDDGRKIYIVYMGNKPKDA-ASTSSHHMR 65
N +S+ ++ LV+LL +S++ D+ ++YIVYMG+ A +S HM
Sbjct: 3 NLAASTCLYSWLLVLLLSSVSAII--------DEDTQVYIVYMGSLSSRADYIPTSDHMS 62
Query: 66 MLEEVIGSNFAPEALLHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTTRS 125
+L++V G + L+ SYKRSFNGF +LTE E I+ EGVVSVFPN +HTT S
Sbjct: 63 ILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTS 122
Query: 126 WDFMGFT--KDVPRVNQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNF 185
WDFMG K+ R +ESD ++GV+DTGIWPES SFSD G+GPPP KWKG C NF
Sbjct: 123 WDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNF 182
Query: 186 TCNKKIIGARAYRSDNFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARG 245
TCN K+IGAR Y S + RD+ GHGTHTAST AG V S +G+ GT RG
Sbjct: 183 TCNNKLIGARDYTS---------EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRG 242
Query: 246 GVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAF 305
GVP++RIA YK+C GCS +L++FDDAIADGVD+I++S+G P + +D IAIGAF
Sbjct: 243 GVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAF 302
Query: 306 HSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTIN 365
H+M GILT +SAGN GP T+ + +PW +VAAST +R +++V LGN G ++N
Sbjct: 303 HAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVN 362
Query: 366 TFDLLEKQYPLIYAGDAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIY 425
FD+ K+YPL+Y A + + C+ ++ + V+GKIL+C PS +
Sbjct: 363 AFDMKGKKYPLVYGKSA--ASSACDAKTAALCAPACLNKSRVKGKILVCG---GPSGYKI 422
Query: 426 FSNAAGVVMNDAGVK-DRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDT 485
+ + + D + D A ++ LP+S L+ D + +Y+ S P A +LK++ + +
Sbjct: 423 AKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNR 482
Query: 486 SAPLIVSFSSRGPNPETLDILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISGT 545
++P+I SFSSRGPN +DILKPD+TAPGVEILAA+ P S D+R+ Y++ SGT
Sbjct: 483 TSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGT 542
Query: 546 SMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELN--IQAEFAYGAGHINPLQ 605
SM+CPH A YVKTF+P WSP+ I+SA+MTTA P+ A+ EFAYGAGH++P+
Sbjct: 543 SMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMA 602
Query: 606 AINPGLVYDANENDYVKFLCGQNYTTAMVQRLSNDNSICNSANFGRVWDLNYPSFA--LS 665
A+NPGLVY+ ++ D++ FLCG NYT+ ++ +S D C+ N +LNYPS + LS
Sbjct: 603 ALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLS 662
Query: 666 STPSEFINQIFTRTLTNVESKASTYTSTVL-GAPQGLIITVNPQVLSFDGIGQTKSFTLT 725
T S F + F RTLTNV + STY S V+ G L I V P VL F + + +SF++T
Sbjct: 663 GTDSTF-SVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVT 722
Query: 726 IRGTISQSIV--SASLVWSDSSHNVRSPITVYIV 747
+ G+ S V SA+L+WSD +HNVRSPI VYI+
Sbjct: 723 VTGSDVDSEVPSSANLIWSDGTHNVRSPIVVYIM 731
BLAST of Clc07G03720 vs. TAIR 10
Match:
AT3G46850.1 (Subtilase family protein )
HSP 1 Score: 615.1 bits (1585), Expect = 7.2e-176
Identity = 344/740 (46.49%), Postives = 465/740 (62.84%), Query Frame = 0
Query: 19 LLGLISSLFASSFNS--NNDDGRKIYIVYMGNKPKDA-ASTSSHHMRMLEEVIGSNFAPE 78
LL I +L SF S +D +++YIVYMG P SHH +L++V G + +
Sbjct: 9 LLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQD 68
Query: 79 ALLHSYKRSFNGFVVKLTEEEAHKISAKEGVVSVFPNGKKHVHTTRSWDFMGFT--KDVP 138
L+ +YKRSFNGF +LTE E +++ + VVSVFP+ ++ TT SW+FMG K
Sbjct: 69 RLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTK 128
Query: 139 RVNQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQTSTNFTCNKKIIGARAYR 198
R +ESD ++GV+D+GI+PES SFS G+GPPP KWKG C+ TNFTCN K+IGAR Y
Sbjct: 129 RNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYYT 188
Query: 199 SD-NFFPPQDIQSPRDSEGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKI 258
FP +S RD+ GHG+HTAS AG V S YGL GT RGGVP+ARIAVYK+
Sbjct: 189 PKLEGFP----ESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKV 248
Query: 259 CWSD--GCSDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAFHSMKHGILTS 318
C C+ ILAAFDDAIAD VDII++S+G + D++AIGAFH+M GILT
Sbjct: 249 CDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTV 308
Query: 319 NSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSQVQLGNMNIYQGYTINTFDLLEKQYP 378
N AGN+GP+ TI + +PW +VAAS ++R +++V LGN G ++N+FDL K+YP
Sbjct: 309 NGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYP 368
Query: 379 LIYAGDAPNITGGFTGSISRFCSANSVDGNLVRGKILLCDSALAPSTFIYFSNAAGVVMN 438
L+Y A + S + FCS +D V+GKI+LCD+ P A +V N
Sbjct: 369 LVYGKSA---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRN 428
Query: 439 DAGVKDRANSYPLPSSYLETADGNDIKTYMTSNGVPTATILKSDAVNDTSAPLIVSFSSR 498
+D A+ + P S L D N + +Y+ S P A +LKS+ + + AP++ S+SSR
Sbjct: 429 P--YEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSR 488
Query: 499 GPNPETLDILKPDLTAPGVEILAAWPPIAPVSSGVIDSRKTMYNIISGTSMSCPHATAAA 558
GPNP DILKPD+TAPG EILAA+ P P S D+R Y +ISGTSMSCPH A
Sbjct: 489 GPNPLIHDILKPDITAPGSEILAAYSPYVPPSES--DTRHVKYTVISGTSMSCPHVAGVA 548
Query: 559 VYVKTFHPTWSPAAIKSALMTTAIPLNAEL---NIQAEFAYGAGHINPLQAINPGLVYDA 618
Y+KTFHP WSP+ I+SA+MTTA P+NA N AEFAYGAGH++P+ AI+PGLVY+A
Sbjct: 549 AYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEA 608
Query: 619 NENDYVKFLCGQNYTTAMVQRLSNDNSICNSANFGRVW-DLNYPSFALSSTPSEFINQIF 678
N++D++ FLCG NYT ++ +S D+S C + +LNYPS + + ++ F
Sbjct: 609 NKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTF 668
Query: 679 TRTLTNVESKASTYTSTVLGAPQGLIITVNPQVLSFDGIGQTKSFTLTIRGT--ISQSIV 738
RT+TNV +TY + V+G+ L + V P VLS + + KSFT+T+ G ++++V
Sbjct: 669 RRTVTNVGRPNATYKAKVVGSK--LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLV 728
Query: 739 SASLVWSDSSHNVRSPITVY 745
SA L+WSD H VRSPI VY
Sbjct: 729 SAQLIWSDGVHFVRSPIVVY 735
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q39547 | 1.5e-266 | 63.32 | Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1 | [more] |
| Q9FIF8 | 1.9e-181 | 46.28 | Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... | [more] |
| Q9FGU3 | 2.2e-177 | 45.45 | Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S... | [more] |
| Q9FIG2 | 6.3e-177 | 46.74 | Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... | [more] |
| Q8L7D2 | 1.2e-175 | 45.76 | Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0KLY4 | 0.0e+00 | 85.85 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G187860 PE=3 SV=1 | [more] |
| A0A1S3CF99 | 0.0e+00 | 84.53 | cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500258 PE=3 SV=1 | [more] |
| A0A6J1FQ02 | 0.0e+00 | 83.04 | cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447181 PE=3 SV=1 | [more] |
| A0A6J1IQ27 | 0.0e+00 | 82.75 | cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111478984 PE=3 SV=1 | [more] |
| A0A6J1FV97 | 0.0e+00 | 82.61 | cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447169 PE=3 SV=1 | [more] |
