Clc03G01930 (gene) Watermelon (cordophanus) v2

BLAST of Clc03G01930 vs. NCBI nr
Match: XP_038894524.1 (E3 UFM1-protein ligase 1 homolog isoform X1 [Benincasa hispida])

HSP 1 Score: 1453.7 bits (3762), Expect = 0.0e+00
Identity = 771/814 (94.72%), Postives = 791/814 (97.17%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRIL+FELLHTVTGKEYITPEH
Sbjct: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDS 120
           LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQI+SDDPRLTLIQGEIISQSYWDS
Sbjct: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIISDDPRLTLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLEGGQLYTPAYVAR 180
           VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQR+GTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEILG 240
           VSAMVRGATRAITVPTNLTVIWSSLQQ+LQGIDGASGIAVDGSFFQSLFN IVKE EILG
Sbjct: 181 VSAMVRGATRAITVPTNLTVIWSSLQQILQGIDGASGIAVDGSFFQSLFNAIVKENEILG 240

Query: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISY+FLRKLGIPNPIQFLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYDFLRKLGIPNPIQFLQSRYPDGIPLST 300

Query: 301 TFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360
           TFIHPSIIEMLDSTV+DILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL
Sbjct: 301 TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360

Query: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGNDPSMSAEPIEM 420
           IFGDSFIFSNIFIKDLY RMEKEMETINVPGSSTGI S DSLSSSK GNDPSMSAE IE 
Sbjct: 361 IFGDSFIFSNIFIKDLYGRMEKEMETINVPGSSTGISSVDSLSSSKFGNDPSMSAESIET 420

Query: 421 GNDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQESSTKSKKNQRKAKATSNVQVA 480
           GND GKTGEIVEK+SKKKKGKS GNTQSIAA+GALDD E+S KSKKNQRKAKATSNVQVA
Sbjct: 421 GNDIGKTGEIVEKKSKKKKGKSFGNTQSIAADGALDDHETSMKSKKNQRKAKATSNVQVA 480

Query: 481 ETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEEHGIDDPAIIVEPLVNHLRPML 540
           ETKAGGKKESVK KENNINYPTE WVIEKIKTLIPDLEEHGIDDPAII++PLVNHLRPML
Sbjct: 481 ETKAGGKKESVKAKENNINYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540

Query: 541 NNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 600
           NNSW+ERRKALFTENAEKMKRLLD+TQQKLDE FLNLQLYEKALDLFEDDQSISVILHRH
Sbjct: 541 NNSWKERRKALFTENAEKMKRLLDNTQQKLDEPFLNLQLYEKALDLFEDDQSISVILHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGERITIAKSFPGSLSNMA 660
           LLRTTAAPIVDMLFHNLDLY KLKNGIE+AELQ+SESVSLNTGER TIAKSFPGSLSN A
Sbjct: 601 LLRTTAAPIVDMLFHNLDLYKKLKNGIEVAELQNSESVSLNTGERTTIAKSFPGSLSNKA 660

Query: 661 VTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSYRKDLTSQVSAEMDAI 720
           VT AEALEGKRVETFI+ALGDLVEESG+I KKLDKKLERTLLHSYRK+LTSQVSAEMD I
Sbjct: 661 VTAAEALEGKRVETFISALGDLVEESGLILKKLDKKLERTLLHSYRKELTSQVSAEMDPI 720

Query: 721 SLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKILSDYQTATVTLLSLIS 780
           +LLPKVVSLLYIQIHHKALQAPGRAIS AISRLKDKLDDSA+KILSDYQTATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYIQIHHKALQAPGRAISVAISRLKDKLDDSAFKILSDYQTATVTLLSLIS 780

Query: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLS 815
           AAVGDEDDCSSDRILTKREFLESQIPALKGLV S
Sbjct: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVSS 814

BLAST of Clc03G01930 vs. NCBI nr
Match: XP_011658001.1 (E3 UFM1-protein ligase 1 homolog [Cucumis sativus] >KGN66067.2 hypothetical protein Csa_019872 [Cucumis sativus])

HSP 1 Score: 1447.2 bits (3745), Expect = 0.0e+00
Identity = 764/815 (93.74%), Postives = 792/815 (97.18%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRIL+FELLHTVTGKEYITPEH
Sbjct: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDS 120
           LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDP+LTLIQGEIISQSYWDS
Sbjct: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPQLTLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLEGGQLYTPAYVAR 180
           VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQR+GTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEILG 240
           VSAMVRGATRAITVPTNLTVIWS+LQQLLQGIDGASGIAVD SFFQSLFNGI+KE E+LG
Sbjct: 181 VSAMVRGATRAITVPTNLTVIWSTLQQLLQGIDGASGIAVDASFFQSLFNGIMKENEVLG 240

Query: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISY+FLRKLGIPNPIQ+LQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYDFLRKLGIPNPIQYLQSRYPDGIPLST 300

Query: 301 TFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360
           TFIHPSIIEMLDST++DILERGSW NSLLVLPSSFEPQDASKILLSCPSVQ ALKSNKAL
Sbjct: 301 TFIHPSIIEMLDSTIEDILERGSWANSLLVLPSSFEPQDASKILLSCPSVQGALKSNKAL 360

Query: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGNDPSMSAEPIEM 420
           IFGDSFIFSN FIKDLYDRMEKEMETI VPGSSTGIFSGDS SSSKLGNDPSMS E IE 
Sbjct: 361 IFGDSFIFSNTFIKDLYDRMEKEMETITVPGSSTGIFSGDSQSSSKLGNDPSMSTESIET 420

Query: 421 GNDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQESSTKSKKNQRKAKATSNVQVA 480
           GNDSGKTG+I++K+SKKKKGKSIGNTQS AAEGALDDQESSTKSKKNQRK + TSNVQVA
Sbjct: 421 GNDSGKTGDIMDKKSKKKKGKSIGNTQSTAAEGALDDQESSTKSKKNQRKTRGTSNVQVA 480

Query: 481 ETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEEHGIDDPAIIVEPLVNHLRPML 540
           ETKAGGKKES KTKE+NINYPTE WVIEKIKTLIPDLEEHGIDDP IIV+PL NHLRPML
Sbjct: 481 ETKAGGKKESAKTKESNINYPTEEWVIEKIKTLIPDLEEHGIDDPTIIVQPLANHLRPML 540

Query: 541 NNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 600
           NN WRERRKALFTENAEKMKRLLD+TQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH
Sbjct: 541 NNLWRERRKALFTENAEKMKRLLDNTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGERITIAKSFPGSLSNMA 660
           LLRTTAAPIVDMLFHNLDLYNKLKNGIE+AELQ+SE+V+L+TGER TIAKSFPGSLSN A
Sbjct: 601 LLRTTAAPIVDMLFHNLDLYNKLKNGIEVAELQNSEAVALSTGERTTIAKSFPGSLSNKA 660

Query: 661 VTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSYRKDLTSQVSAEMDAI 720
           VTVAEALEGKRVETFINALGDLVEESGMI KKLDKKLERTLLHSYRK+LTSQVSAEMD I
Sbjct: 661 VTVAEALEGKRVETFINALGDLVEESGMIPKKLDKKLERTLLHSYRKELTSQVSAEMDPI 720

Query: 721 SLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKILSDYQTATVTLLSLIS 780
           +LLPKVVSLLY+QI+HKALQAPGRAIS AISRLKDKLDDSA+KILSDYQTATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYVQIYHKALQAPGRAISVAISRLKDKLDDSAHKILSDYQTATVTLLSLIS 780

Query: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA 816
           AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA
Sbjct: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA 815

BLAST of Clc03G01930 vs. NCBI nr
Match: XP_008457514.1 (PREDICTED: E3 UFM1-protein ligase 1 homolog [Cucumis melo])

HSP 1 Score: 1446.8 bits (3744), Expect = 0.0e+00
Identity = 765/815 (93.87%), Postives = 790/815 (96.93%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRIL+FELLHTVTGKEYITPEH
Sbjct: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDS 120
           LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDP+LTLIQGEIISQSYWDS
Sbjct: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPQLTLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLEGGQLYTPAYVAR 180
           VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQR+GTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEILG 240
           VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVD SFFQSLFNGI+KE E+LG
Sbjct: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDASFFQSLFNGIMKENEVLG 240

Query: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300

Query: 301 TFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360
           TFIHPSIIEMLDS ++DILERGSW NSLLVLPSSFEPQDASK+LLSCPSVQ ALKSNKAL
Sbjct: 301 TFIHPSIIEMLDSAIEDILERGSWANSLLVLPSSFEPQDASKVLLSCPSVQGALKSNKAL 360

Query: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGNDPSMSAEPIEM 420
           IFGDSFIFSNIFIKDL+DRMEKEMETI VPGSSTGIFSGDS SSSKLG DPSMS E IEM
Sbjct: 361 IFGDSFIFSNIFIKDLFDRMEKEMETIIVPGSSTGIFSGDSQSSSKLGIDPSMSTESIEM 420

Query: 421 GNDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQESSTKSKKNQRKAKATSNVQVA 480
           GNDSGKTGEIVEK+SKKKKGKS+GNTQS  AEGALDDQESSTKSKKNQRK + TSNVQVA
Sbjct: 421 GNDSGKTGEIVEKKSKKKKGKSVGNTQSTTAEGALDDQESSTKSKKNQRKTRGTSNVQVA 480

Query: 481 ETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEEHGIDDPAIIVEPLVNHLRPML 540
           ETK GGKKESVKTKE+NINYPTE WVIEKIKTLIPDLEEHGIDDP IIV+PL NHLRPML
Sbjct: 481 ETKTGGKKESVKTKESNINYPTEEWVIEKIKTLIPDLEEHGIDDPTIIVQPLANHLRPML 540

Query: 541 NNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 600
           NN WRERRKALFTENAEKMK LLD+TQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH
Sbjct: 541 NNLWRERRKALFTENAEKMKHLLDNTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGERITIAKSFPGSLSNMA 660
           LLRTTAAPIVDMLFHNLDLYNKLKNGIE+AELQ+SE V+L+TGERITIAKSFPGSLSN A
Sbjct: 601 LLRTTAAPIVDMLFHNLDLYNKLKNGIEVAELQNSEPVALSTGERITIAKSFPGSLSNKA 660

Query: 661 VTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSYRKDLTSQVSAEMDAI 720
           VTVAEALEGKRVETFINALGDLVEESGMI KKLDKKLERTLLHSYRK+LTSQVS+EMD I
Sbjct: 661 VTVAEALEGKRVETFINALGDLVEESGMISKKLDKKLERTLLHSYRKELTSQVSSEMDPI 720

Query: 721 SLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKILSDYQTATVTLLSLIS 780
           +LLPKVVSLLY+QI+HKALQAPGRAIS AISRLKDKLDDSA+KILSDYQTATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYVQIYHKALQAPGRAISVAISRLKDKLDDSAHKILSDYQTATVTLLSLIS 780

Query: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA 816
           AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA
Sbjct: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA 815

BLAST of Clc03G01930 vs. NCBI nr
Match: XP_023519824.1 (E3 UFM1-protein ligase 1 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1427.9 bits (3695), Expect = 0.0e+00
Identity = 754/815 (92.52%), Postives = 787/815 (96.56%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEH 60
           MD ELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRIL+FELLHTVTGKEYITPEH
Sbjct: 1   MDAELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDS 120
           LRREILAEIEKLGRISLIDLAD IGVDLYYIEKQAEQI+SDDPRL LIQGEIISQSYWDS
Sbjct: 61  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLEGGQLYTPAYVAR 180
            AEEINERLQESSQIALAEIAA+LQVGSELLASML QR+GTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 AAEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEILG 240
           VSAMVRGATRAITVPTNLTVIWSSLQQLL GIDGASGIAVDGSFFQSLFNG+VKE E+LG
Sbjct: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 240

Query: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTP IFSIAQKE IDSFFSQNSVI+YEFLRKLGIPNPIQFLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPKIFSIAQKECIDSFFSQNSVINYEFLRKLGIPNPIQFLQSRYPDGIPLST 300

Query: 301 TFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360
           TFIHPSIIEMLDSTV+DILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALK NKA 
Sbjct: 301 TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKCNKAF 360

Query: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGNDPSMSAEPIEM 420
           IFGDSFIFSNIFIKDLYDRMEKEMET+NVPGSST   SGDSLS SK+GNDPSMSAE IE 
Sbjct: 361 IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANISGDSLSISKVGNDPSMSAESIEP 420

Query: 421 GNDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQESSTKSKKNQRKAKATSNVQVA 480
           GN+SGK GEI+EK+SKKKKGKS GNTQ+IAAEGALDDQESSTKSKKNQRKAKAT++VQVA
Sbjct: 421 GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATTSVQVA 480

Query: 481 ETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEEHGIDDPAIIVEPLVNHLRPML 540
           ETKAGGKKESVKTKE+NINYPTE WVIEK+KTLIPDL+EHGIDDPA I++PLVNHLRPML
Sbjct: 481 ETKAGGKKESVKTKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATILQPLVNHLRPML 540

Query: 541 NNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 600
           NNSWRERRKALFTENAEKMKRLLDSTQQKLDESF+NLQLYEKALDLFEDDQSISV+LHRH
Sbjct: 541 NNSWRERRKALFTENAEKMKRLLDSTQQKLDESFINLQLYEKALDLFEDDQSISVVLHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGERITIAKSFPGSLSNMA 660
           LLRT+AAPIVDMLFHNLDLYNKLKNGIE AE+Q+SE VSL+TGER TIAKSFPGSLSN A
Sbjct: 601 LLRTSAAPIVDMLFHNLDLYNKLKNGIEAAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA 660

Query: 661 VTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSYRKDLTSQVSAEMDAI 720
           VTVAEALEGKRVETF++ALGDLVEESGMI KKLDKKLERTLLHSYRKDLTSQVSAEMD I
Sbjct: 661 VTVAEALEGKRVETFVHALGDLVEESGMISKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720

Query: 721 SLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKILSDYQTATVTLLSLIS 780
           +LLPKVVSLLY+QIHHKALQAPGRAIS AISRLKDKLD+SAYKILSDYQ+ATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYVQIHHKALQAPGRAISVAISRLKDKLDESAYKILSDYQSATVTLLSLIS 780

Query: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA 816
           AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA
Sbjct: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA 815

BLAST of Clc03G01930 vs. NCBI nr
Match: KAG6583453.1 (E3 UFM1-protein ligase 1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1422.1 bits (3680), Expect = 0.0e+00
Identity = 749/815 (91.90%), Postives = 786/815 (96.44%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEH 60
           MD ELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRIL+FELLHTVTGKEYITPEH
Sbjct: 1   MDAELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDS 120
           LR EILAEIEKLGRISLIDLAD IGVDLYYIEKQAEQI+SDDPRL LIQGEIISQSYWDS
Sbjct: 61  LRHEILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLEGGQLYTPAYVAR 180
            AEEINERLQESSQIALAEIAAELQVGSELLASML QR+GTLVKGRLEGGQLYTPAYV+R
Sbjct: 121 AAEEINERLQESSQIALAEIAAELQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVSR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEILG 240
           VSAMVRGATRAITVPTNLTVIWSSLQQLL GIDGASGIAVDGSFFQSLFNG+VKE E+LG
Sbjct: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 240

Query: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTP IFSIAQKE IDSFFSQNSVI+YEFLRKLGIPNPIQFLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPKIFSIAQKECIDSFFSQNSVINYEFLRKLGIPNPIQFLQSRYPDGIPLST 300

Query: 301 TFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360
           TFIHPSIIEMLDSTV+DILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALK NKA 
Sbjct: 301 TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKCNKAF 360

Query: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGNDPSMSAEPIEM 420
           IFGDSFIFSNIFIKDLYDRMEKEMET+NVPGSST  FSGDSLS SK+GNDPSMSAE IE 
Sbjct: 361 IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 420

Query: 421 GNDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQESSTKSKKNQRKAKATSNVQVA 480
           GN+SGK GEI+EK+SKKKKGKS GNTQ++AAEG LDDQESSTKSKKNQRKAKAT++VQVA
Sbjct: 421 GNNSGKIGEIMEKKSKKKKGKSTGNTQTVAAEGVLDDQESSTKSKKNQRKAKATTSVQVA 480

Query: 481 ETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEEHGIDDPAIIVEPLVNHLRPML 540
           ETKAGGKKESVKTKE+NIN+PTE WVIEK+KTLIPDL+EHGIDDPA I++PLVNHLRPML
Sbjct: 481 ETKAGGKKESVKTKESNINHPTEEWVIEKLKTLIPDLDEHGIDDPATILQPLVNHLRPML 540

Query: 541 NNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 600
           NNSWRERRKALFTENAEKMKRLLDSTQQKLDESF+NLQLYEKALDLFEDDQSISV+LHRH
Sbjct: 541 NNSWRERRKALFTENAEKMKRLLDSTQQKLDESFINLQLYEKALDLFEDDQSISVVLHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGERITIAKSFPGSLSNMA 660
           LLRT+AAPIVDMLFHNLDLYNKLKNGIE+AE+Q+SE VSL+ GER TIAKSFPGSLSN A
Sbjct: 601 LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSIGERTTIAKSFPGSLSNKA 660

Query: 661 VTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSYRKDLTSQVSAEMDAI 720
           VTVAEALEGKRVETF++ALGD+VEESGMI KKLDKKLERTLLHSYRKDLTSQVSAEMD I
Sbjct: 661 VTVAEALEGKRVETFMHALGDIVEESGMISKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720

Query: 721 SLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKILSDYQTATVTLLSLIS 780
           +LLPKVVSLLY+QIHHKALQAPGRAIS AISRLKDKLD+SAYKILSDYQ+ATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYVQIHHKALQAPGRAISVAISRLKDKLDESAYKILSDYQSATVTLLSLIS 780

Query: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA 816
           AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA
Sbjct: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA 815

BLAST of Clc03G01930 vs. ExPASy Swiss-Prot
Match: Q9LX73 (E3 UFM1-protein ligase 1 homolog OS=Arabidopsis thaliana OX=3702 GN=At3g46220 PE=1 SV=1)

HSP 1 Score: 969.5 bits (2505), Expect = 2.3e-281
Identity = 513/814 (63.02%), Postives = 646/814 (79.36%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQ KSS+RLS+RNVVELVQKLQEL +++F+LLHTVTGKEYIT E 
Sbjct: 1   MDDELLELQRQFEFAQQVKSSVRLSDRNVVELVQKLQELGVIDFDLLHTVTGKEYITQEQ 60

Query: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDS 120
           LR EI  EI KLGR+S+IDLADTIGVDLY++EKQA+ +V +DP L L+QGEIISQSYWDS
Sbjct: 61  LRNEITREISKLGRVSVIDLADTIGVDLYHVEKQAQDVVLNDPGLMLVQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLEGGQLYTPAYVAR 180
           +AEEINERLQE SQIA+AE+A +LQVGSEL+ S+L+ R+GTLVK RLEGGQLYTPAYV R
Sbjct: 121 IAEEINERLQECSQIAVAELAGQLQVGSELVQSVLEPRLGTLVKARLEGGQLYTPAYVER 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEILG 240
           V+AMVRGA+R I VP+NL+ +W+ LQQL+Q  +GASG+AV+ SFFQS+FN ++KE+E+LG
Sbjct: 181 VTAMVRGASRGIFVPSNLSALWAPLQQLVQETNGASGVAVENSFFQSIFNRLLKEEEMLG 240

Query: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300
           SLRAG HWTP+ F+ AQKE +DS FSQNS ISYE ++KLGI   +QFLQSRYPDG PL+ 
Sbjct: 241 SLRAGTHWTPSAFATAQKECVDSSFSQNSYISYESMQKLGISQAVQFLQSRYPDGTPLAA 300

Query: 301 TFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360
            FIH S+IEMLDS  +D +E+ SW +SL VLPSSF  QDA+K+LL CPSVQ ALK+ KAL
Sbjct: 301 VFIHSSMIEMLDSATEDAIEQNSWIDSLSVLPSSFTSQDANKMLLLCPSVQSALKAEKAL 360

Query: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGNDPSMSAEPIEM 420
           I G+S++ S+ FIK +YD++EKE +  ++  S+  +    S SS         S E I  
Sbjct: 361 ILGESYVLSSGFIKGIYDQIEKEADAFSIQASTATLIHPSSKSSE--------STESIPA 420

Query: 421 GNDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQESS-TKSKKNQRKAKATSNVQV 480
             D G         SKKKKGKS  +T++   E   DD+E +  KSK+NQ+K + +S+ Q 
Sbjct: 421 NTDKG---------SKKKKGKS-ASTKAATVETVPDDEEDARPKSKRNQKKGRDSSSSQK 480

Query: 481 AETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEEHGIDDPAIIVEPLVNHLRPM 540
            ++KAGGKKESVK +E+N   P + WV++KI   +P+ E+ G ++P  I++ L +H++PM
Sbjct: 481 LDSKAGGKKESVKAQESNNIIPPDEWVMKKIVDSVPEFEDDGTENPDSILKHLADHMKPM 540

Query: 541 LNNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNLQLYEKALDLFEDDQSISVILHR 600
           L NS +ERRK +FTENA++M+RL+D  Q+KLDESFLN+QLYEKALDLFEDDQS +V+LHR
Sbjct: 541 LINSLKERRKKIFTENADRMRRLIDDLQKKLDESFLNMQLYEKALDLFEDDQSTAVVLHR 600

Query: 601 HLLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGERITIAKSFPGSLSNM 660
           HLLRTTAA I D L H LD++NK+KNG E+ E ++ + V L++ ER  +AK+  GSLS  
Sbjct: 601 HLLRTTAATIADTLLHGLDIHNKMKNGTEVEESKTQDLVLLDSSERTALAKNLNGSLSKK 660

Query: 661 AVTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSYRKDLTSQVSAEMDA 720
           A+ + EALEGKRV+TF+    DL EESG++ KKLDKKLERTLLHSYRKDL SQVS E D 
Sbjct: 661 ALALVEALEGKRVDTFMVTFRDLAEESGLVLKKLDKKLERTLLHSYRKDLISQVSTESDP 720

Query: 721 ISLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKILSDYQTATVTLLSLI 780
           I+LL KVVSLL+I+IH+KALQAPGRAI+AAIS LK+KLD+SAYK L+DYQTATVTLL+L+
Sbjct: 721 IALLAKVVSLLFIKIHNKALQAPGRAIAAAISHLKEKLDESAYKTLTDYQTATVTLLALM 780

Query: 781 SAAVGDEDDCSSDRILTKREFLESQIPALKGLVL 814
           SA+ G+E DCS+DRILTKRE LESQ+P L+ LVL
Sbjct: 781 SASSGEEHDCSADRILTKRELLESQMPLLRTLVL 796

BLAST of Clc03G01930 vs. ExPASy Swiss-Prot
Match: B8AXB6 (E3 UFM1-protein ligase 1 homolog OS=Oryza sativa subsp. indica OX=39946 GN=OsI_18219 PE=3 SV=1)

HSP 1 Score: 849.7 bits (2194), Expect = 2.7e-245
Identity = 453/817 (55.45%), Postives = 616/817 (75.40%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEH 60
           MD ELLELQRQ E AQ A+SS+RLSERNVVELVQKLQE  I++FELLHT +GKEYIT +H
Sbjct: 1   MDAELLELQRQLEAAQSARSSVRLSERNVVELVQKLQERGIIDFELLHTTSGKEYITSDH 60

Query: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDS 120
           L+ EI  EI+K GR SL+DL+D +GVDLY++E+Q++++V+DDP L LI GEI+SQSYWD+
Sbjct: 61  LKHEIKMEIKKRGRASLVDLSDILGVDLYHVERQSQKVVADDPSLMLINGEIMSQSYWDT 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLEGGQLYTPAYVAR 180
           V EEINE+LQE SQIALAEIAA+L +GSEL+ ++L+ R+GT+VKGRLEGGQLYTPAYV+R
Sbjct: 121 VTEEINEKLQERSQIALAEIAAQLHIGSELVVNILEPRLGTIVKGRLEGGQLYTPAYVSR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEILG 240
           ++AMVRGA R ITVPTNL  +W+SLQ  LQ + GASG++V+GSFFQS+FNG++KE  +LG
Sbjct: 181 ITAMVRGAARGITVPTNLPSVWNSLQLQLQEMHGASGVSVEGSFFQSIFNGLLKEGVVLG 240

Query: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300
           S+RAGV WTP +F+ AQKES+D+FFSQNS I YE L+KL IP P Q+L++RYPDGI L  
Sbjct: 241 SVRAGVQWTPAVFAHAQKESVDAFFSQNSYIGYEVLQKLAIPQPKQYLEARYPDGIALEA 300

Query: 301 TFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360
            F+HPS+++MLD+ V D +E G W ++L VLPS     DA+KIL  CPS+Q A+KS+KA+
Sbjct: 301 VFVHPSVVDMLDAAVGDTIENGQWIDALSVLPSYITGPDATKILSLCPSLQKAIKSSKAV 360

Query: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGNDPSMSAEPIEM 420
           +FG+S +FSN FIK ++DR+EKEM++  +  S+      +  S  ++G+D        ++
Sbjct: 361 VFGESCVFSNAFIKGIFDRLEKEMDSFGIKHSAGQGKPSNMSSEHRIGSDGK------DL 420

Query: 421 G-NDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQES-STKSKKNQRKAKATSNVQ 480
           G ND+   G   +K  KKK+GK  G+ +  A E   D++ES   K KK  RK K   +  
Sbjct: 421 GDNDTSSIGASSDKGPKKKRGKVSGSAKGAAVEKDDDNEESIPVKGKKAHRKNKDAGS-- 480

Query: 481 VAETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEE-HGIDDPAIIVEPLVNHLR 540
             + K GGKK S KTKE+N N   +  + +K+ T+ P+LEE  G DD    ++ L +HLR
Sbjct: 481 SGDAKHGGKKASEKTKEDNTNIFPDDLIEQKVLTVAPELEELGGSDDLNGPLKLLSSHLR 540

Query: 541 PMLNNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNLQLYEKALDLFEDDQSISVIL 600
           PML ++W ++R  + +ENAE+ +RLLD+ Q++LDE+ L++QLYEK+LD+FEDD + S IL
Sbjct: 541 PMLMDAWMKKRNTMLSENAERRRRLLDNLQKQLDEAVLDMQLYEKSLDVFEDDPATSAIL 600

Query: 601 HRHLLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGERITIAKSFPGSLS 660
           H+HLLRT  AP+VD +   L   NKLKNG+++ +  S E+V L+T +R ++AK  PGSLS
Sbjct: 601 HKHLLRTMGAPVVDKILLTLHKDNKLKNGMDVED--SEENVQLSTADRTSLAKDLPGSLS 660

Query: 661 NMAVTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSYRKDLTSQVSAEM 720
             A  +AE LEGKR ++F++AL D  EESG++FKKLDK+LER++LHSYRKDLT+QVS+E 
Sbjct: 661 VKAQALAETLEGKRFDSFMDALRDTAEESGLLFKKLDKRLERSMLHSYRKDLTAQVSSEN 720

Query: 721 DAISLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKILSDYQTATVTLLS 780
           D IS LPKVV+LL++Q ++KALQAPGRA+ A I+ LKDK+    YK+L+DY + TV +L+
Sbjct: 721 DPISFLPKVVALLFLQAYNKALQAPGRAVGAVIALLKDKIPAPTYKVLADYHSTTVKVLA 780

Query: 781 LISAAVGDEDDCSSDRILTKREFLESQI-PALKGLVL 814
           L +AA  D +DC++DR+L ++E LE ++ P LK LVL
Sbjct: 781 LQAAATEDGEDCATDRMLERKEDLEERLMPELKSLVL 807

BLAST of Clc03G01930 vs. ExPASy Swiss-Prot
Match: B9FM64 (E3 UFM1-protein ligase 1 homolog OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0117600 PE=3 SV=1)

HSP 1 Score: 849.7 bits (2194), Expect = 2.7e-245
Identity = 453/817 (55.45%), Postives = 616/817 (75.40%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEH 60
           MD ELLELQRQ E AQ A+SS+RLSERNVVELVQKLQE  I++FELLHT +GKEYIT +H
Sbjct: 1   MDAELLELQRQLEAAQSARSSVRLSERNVVELVQKLQERGIIDFELLHTTSGKEYITSDH 60

Query: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDS 120
           L+ EI  EI+K GR SL+DL+D +GVDLY++E+Q++++V+DDP L LI GEI+SQSYWD+
Sbjct: 61  LKHEIKMEIKKRGRASLVDLSDILGVDLYHVERQSQKVVADDPSLMLINGEIMSQSYWDT 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLEGGQLYTPAYVAR 180
           V EEINE+LQE SQIALAEIAA+L +GSEL+ ++L+ R+GT+VKGRLEGGQLYTPAYV+R
Sbjct: 121 VTEEINEKLQERSQIALAEIAAQLHIGSELVVNILEPRLGTIVKGRLEGGQLYTPAYVSR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEILG 240
           ++AMVRGA R ITVPTNL  +W+SLQ  LQ + GASG++V+GSFFQS+FNG++KE  +LG
Sbjct: 181 ITAMVRGAARGITVPTNLPSVWNSLQLQLQEMHGASGVSVEGSFFQSIFNGLLKEGVVLG 240

Query: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300
           S+RAGV WTP +F+ AQKES+D+FFSQNS I YE L+KL IP P Q+L++RYPDGI L  
Sbjct: 241 SVRAGVQWTPAVFAHAQKESVDAFFSQNSYIGYEVLQKLAIPQPKQYLEARYPDGIALEA 300

Query: 301 TFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360
            F+HPS+++MLD+ V D +E G W ++L VLPS     DA+KIL  CPS+Q A+KS+KA+
Sbjct: 301 VFVHPSVVDMLDAAVGDTIENGQWIDALSVLPSYITGPDATKILSLCPSLQKAIKSSKAV 360

Query: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGNDPSMSAEPIEM 420
           +FG+S +FSN FIK ++DR+EKEM++  +  S+      +  S  ++G+D        ++
Sbjct: 361 VFGESCVFSNAFIKGIFDRLEKEMDSFGIKHSAGQGKPSNMSSEHRIGSDGK------DL 420

Query: 421 G-NDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQES-STKSKKNQRKAKATSNVQ 480
           G ND+   G   +K  KKK+GK  G+ +  A E   D++ES   K KK  RK K   +  
Sbjct: 421 GDNDTSSIGASSDKGPKKKRGKVSGSAKGAAVEKDDDNEESIPVKGKKAHRKNKDAGS-- 480

Query: 481 VAETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEE-HGIDDPAIIVEPLVNHLR 540
             + K GGKK S KTKE+N N   +  + +K+ T+ P+LEE  G DD    ++ L +HLR
Sbjct: 481 SGDAKHGGKKASEKTKEDNTNIFPDDLIEQKVLTVAPELEELGGSDDLNGPLKLLSSHLR 540

Query: 541 PMLNNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNLQLYEKALDLFEDDQSISVIL 600
           PML ++W ++R  + +ENAE+ +RLLD+ Q++LDE+ L++QLYEK+LD+FEDD + S IL
Sbjct: 541 PMLMDAWMKKRNTMLSENAERRRRLLDNLQKQLDEAVLDMQLYEKSLDVFEDDPATSAIL 600

Query: 601 HRHLLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGERITIAKSFPGSLS 660
           H+HLLRT  AP+VD +   L   NKLKNG+++ +  S E+V L+T +R ++AK  PGSLS
Sbjct: 601 HKHLLRTMGAPVVDKILLTLHKDNKLKNGMDVED--SEENVQLSTADRTSLAKDLPGSLS 660

Query: 661 NMAVTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSYRKDLTSQVSAEM 720
             A  +AE LEGKR ++F++AL D  EESG++FKKLDK+LER++LHSYRKDLT+QVS+E 
Sbjct: 661 VKAQALAETLEGKRFDSFMDALRDTAEESGLLFKKLDKRLERSMLHSYRKDLTAQVSSEN 720

Query: 721 DAISLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKILSDYQTATVTLLS 780
           D IS LPKVV+LL++Q ++KALQAPGRA+ A I+ LKDK+    YK+L+DY + TV +L+
Sbjct: 721 DPISFLPKVVALLFLQAYNKALQAPGRAVGAVIALLKDKIPAPTYKVLADYHSTTVKVLA 780

Query: 781 LISAAVGDEDDCSSDRILTKREFLESQI-PALKGLVL 814
           L +AA  D +DC++DR+L ++E LE ++ P LK LVL
Sbjct: 781 LQAAATEDGEDCATDRMLERKEDLEERLMPELKSLVL 807

BLAST of Clc03G01930 vs. ExPASy Swiss-Prot
Match: Q5ZMG1 (E3 UFM1-protein ligase 1 OS=Gallus gallus OX=9031 GN=UFL1 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 3.2e-73
Identity = 231/831 (27.80%), Postives = 403/831 (48.50%), Query Frame = 0

Query: 3   DELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEHLR 62
           +E+  L   F+ AQ A+++ RLSERN +E+V KL  +   + E++HT+ GKEY+TP  + 
Sbjct: 6   EEIRRLAADFQRAQFAEAAHRLSERNCIEIVTKL--IAEKQLEVVHTLDGKEYVTPAQIS 65

Query: 63  REILAEIEKL-GRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDSV 122
           +EI  E+    GRI+++DL   I VDL +IE +A  IV  D  + L+ G++I++SY D +
Sbjct: 66  KEIWDELSVCGGRINIVDLQQIINVDLLHIENRANDIVKSDKAVQLVLGQLINESYLDQL 125

Query: 123 AEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLE---GGQLYTPAYV 182
           AEEIN++LQE+ Q+ ++E+     +  + L   L +R+G ++ GRL+    G ++T A+V
Sbjct: 126 AEEINDKLQETGQVTISELCKAYDLPGDFLTQALSKRLGRIIHGRLDQENRGVIFTEAFV 185

Query: 183 ARVSAMVRGATRAITVPT---NLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKE 242
           +R  A +RG   AIT PT   NL   +   + LL             S  + L N    +
Sbjct: 186 SRHRARIRGLFSAITRPTPVSNLITRYGFQEHLLY------------SVLEELVNTSRLK 245

Query: 243 KEILGSLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDG 302
             ++G  +    + P+I++  Q   +DSFF QN  + ++ L +LGIP+P  +++ RY   
Sbjct: 246 GTVVGGKQDKAVFVPDIYARTQSNWVDSFFKQNGYLEFDALYRLGIPDPAGYIKKRYKST 305

Query: 303 --IPLSTTFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVA 362
             + L    +   I++ ++++VD+++  GSW +   +LPSS   +D   +L     ++  
Sbjct: 306 KLLFLRAACVGQEIVDRVEASVDEVISSGSWIDVATLLPSSLSVEDIGILLQQV--MRSL 365

Query: 363 LKSNKALIFGDSFIFSNIFIK---DLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGND 422
            K++  L+F D+ + S  FI    DL+  M K+     +  S   + + + L  S +   
Sbjct: 366 NKNSSGLVFSDTIVVSEKFISSCTDLFSDMMKQKAEKEMKNSPVHLITEEDLKQSYV--- 425

Query: 423 PSMSAEPIEMGNDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQESSTKSKKNQRK 482
                  +E    + K     +K+ +++K K+   + S+   G  + +E   K  K + +
Sbjct: 426 -------LENSYTNKK-----DKKDERRK-KATEGSGSVRGGGGGNAREIKIKKTKKKGR 485

Query: 483 AKATSNVQVAETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEEHGIDDPAIIVE 542
             A S+ +   T  G      + K+   ++ ++  + + +KT       H  D P  ++ 
Sbjct: 486 KDADSDEESQATGTG------RNKQLEFHFMSQEEMQDVLKT-------HLQDCPEELIT 545

Query: 543 PLVNHLRPMLNNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNL----QLYEKALDL 602
            L  HL   L  S++E  +++FT +          T + L E F NL    +L+EK    
Sbjct: 546 ELAEHLMRPLTKSYQEVVRSVFTSSTSSSGASGRQTMKDLQEEFSNLYNNIRLFEKGTKY 605

Query: 603 FEDDQSISVILHRHLLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGE-R 662
           F D+   +  L +HLL+T    I +++F          N +    + ++E+ S  T E R
Sbjct: 606 FTDETQTN--LAKHLLKTVCTDITNLIF----------NFLASDSMMTTENYSTITSEVR 665

Query: 663 ITIAKSFPGSLSNMAVTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSY 722
             I    P         +  +L GK +E F++ L    +   ++ KK DKK ER +L  +
Sbjct: 666 TKILGKLPEDTRGPLTKLHTSLNGKSLEDFLSYLDAAADICDIMVKKGDKKKERQVLFQH 725

Query: 723 RKDLTSQVSAEMDAISLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKIL 782
           R+ L  Q+    D   +L     LL+    H  L APGR++   I+ L  K+ +  + +L
Sbjct: 726 RQALIEQLKVTEDPALVLHLTAVLLFQFSTHCMLHAPGRSVPQIINFLSGKIPEDQHSLL 779

Query: 783 SDYQTATVTLLSLISAAVGDEDDCSSDR---ILTKREFLESQIPALKGLVL 814
             YQ   V  L   S     EDD  ++      T R+ L+    ++K LVL
Sbjct: 786 IKYQGLVVKQLISQSKKAEQEDDNKTEEEEGADTIRKELQEITTSVKDLVL 779

BLAST of Clc03G01930 vs. ExPASy Swiss-Prot
Match: B2GV24 (E3 UFM1-protein ligase 1 OS=Rattus norvegicus OX=10116 GN=Ufl1 PE=1 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 1.1e-68
Identity = 225/848 (26.53%), Postives = 409/848 (48.23%), Query Frame = 0

Query: 3   DELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEHLR 62
           +E+  L   F+ AQ A+S+ RLSERN +E+V KL   +  + E++HT+ GKEYITP  + 
Sbjct: 6   EEIRRLAADFQRAQFAESTQRLSERNCIEIVNKLISQK--QLEVVHTLDGKEYITPAQIS 65

Query: 63  REILAEIE-KLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDSV 122
           +E+  E+  + GR++++DL   I VDL +IE +   I+  +  + ++ G+++ ++Y D +
Sbjct: 66  KEMRDELHVRGGRVNIVDLQQVINVDLTHIENRVGDIIKSEKHVQMVLGQLVDENYLDRL 125

Query: 123 AEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLE---GGQLYTPAYV 182
           +EE+N++LQES Q+ ++E+     +  + L   L QR+G ++ G L+    G ++T A+V
Sbjct: 126 SEEVNDKLQESGQVTVSELCKTYDLPGDFLTQALTQRLGRIINGHLDLDNRGVIFTEAFV 185

Query: 183 ARVSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEI 242
           AR  A +RG   AIT PT +  + S                    F + L   +++E   
Sbjct: 186 ARHKARIRGLFSAITRPTAVNSLVSKY-----------------GFQEQLLYSVLEELVS 245

Query: 243 LGSLRAGV--------HWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQS 302
            G LR  V         + P+I+S  Q   +DSFF QN  + ++ L +LGIP+ + +++ 
Sbjct: 246 TGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVNYIKK 305

Query: 303 RYPDG--IPLSTTFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCP 362
           RY +   + L  T +   +++ ++++V++ +  G+W +   +LPSS   +DA+ +L    
Sbjct: 306 RYKNTPLLFLKATCVGQGLVDQVEASVEEAISSGTWVDVSPLLPSSLSVEDAAMLLQQV- 365

Query: 363 SVQVALKSNKALIFGDSFIFSNIFIKD--------LYDRMEKEMETINVPGSSTGIFSGD 422
            ++   K   A +F D+ + S  FI D        ++ + EKEM+      +   + + +
Sbjct: 366 -MRPFGKHASATVFSDTVVVSEKFINDCTKLFSERMHQKAEKEMK-----NNPVHLITEE 425

Query: 423 SLSSSKLGNDPSMSAEPIEMGNDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQES 482
            L    +          +E  N + K     +K+ +++K K+   + S+   G  + +E 
Sbjct: 426 DLKQISI----------LESVNTNKK-----DKKDERRK-KATEGSGSVRGGGGGNAREY 485

Query: 483 STKSKKNQRKAKATSNVQVAETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEEH 542
             K  K + +    S+ +   + AG KK        +I +  +  +   ++  IPD  E 
Sbjct: 486 KIKKVKKKGRKDEDSDDESQSSHAGKKKP-------DITFMFQDEIEGCLRKHIPDAPEE 545

Query: 543 GIDDPAIIVEPLVNHLRPMLNNSWRERRKALFTENAEK----MKRLLDSTQQKLDESFLN 602
            I +       L  HL   LN  + E  +++F  +        KR +   Q+++   + N
Sbjct: 546 FISE-------LAEHLIKPLNKMYLEVVRSVFMSSTSASGTGRKRTIKDLQEEVSNLYNN 605

Query: 603 LQLYEKALDLFEDDQSISVILHRHLLRTTAAPIVDMLFHNL--DLYNKLKNGIEIAELQS 662
           ++L+EK +  F DD   +  L +HLL+T    I +++F+ L  DL   +++   I     
Sbjct: 606 IRLFEKGMKYFADDTQTA--LTKHLLKTVCTDITNLMFNFLASDLMMAVEDPATITSDMR 665

Query: 663 SESVSLNTGERITIAKSFPGSLSNMAVT-VAEALEGKRVETFINALGDLVEESGMIFKKL 722
            + +S  T E            + +A+T +  +L  K +E F++ L    E   ++ KK 
Sbjct: 666 KKILSKLTEE------------TKVALTKLHNSLNEKSIEDFLSCLDSATEACDIMVKKG 725

Query: 723 DKKLERTLLHSYRKDLTSQVSAEMDAISLLPKVVSLLYIQIHHKALQAPGRAISAAISRL 782
           DKK ER +L  +R+ L  Q+    D   +L     LL+    H  L APGR +   I+ L
Sbjct: 726 DKKRERQILFQHRQALADQLKVTEDPALILHLTSVLLFQFSTHSMLHAPGRCVPQIIAFL 783

Query: 783 KDKLDDSAYKILSDYQTATVTLLSLISAAVGDEDDCSSDRI--------LTKREFLESQI 814
            +K+ +  + +L  YQ   V  L   +   G  +D SSD +         T R+ L+   
Sbjct: 786 HNKIPEDQHTLLVKYQGLVVKQLVSQNKKSGQGEDPSSDDLDKEQHDVTNTTRKELQELS 783

BLAST of Clc03G01930 vs. ExPASy TrEMBL
Match: A0A0A0LV94 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G498310 PE=3 SV=1)

HSP 1 Score: 1447.2 bits (3745), Expect = 0.0e+00
Identity = 764/815 (93.74%), Postives = 792/815 (97.18%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRIL+FELLHTVTGKEYITPEH
Sbjct: 31  MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 90

Query: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDS 120
           LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDP+LTLIQGEIISQSYWDS
Sbjct: 91  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPQLTLIQGEIISQSYWDS 150

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLEGGQLYTPAYVAR 180
           VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQR+GTLVKGRLEGGQLYTPAYVAR
Sbjct: 151 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 210

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEILG 240
           VSAMVRGATRAITVPTNLTVIWS+LQQLLQGIDGASGIAVD SFFQSLFNGI+KE E+LG
Sbjct: 211 VSAMVRGATRAITVPTNLTVIWSTLQQLLQGIDGASGIAVDASFFQSLFNGIMKENEVLG 270

Query: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISY+FLRKLGIPNPIQ+LQSRYPDGIPLST
Sbjct: 271 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYDFLRKLGIPNPIQYLQSRYPDGIPLST 330

Query: 301 TFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360
           TFIHPSIIEMLDST++DILERGSW NSLLVLPSSFEPQDASKILLSCPSVQ ALKSNKAL
Sbjct: 331 TFIHPSIIEMLDSTIEDILERGSWANSLLVLPSSFEPQDASKILLSCPSVQGALKSNKAL 390

Query: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGNDPSMSAEPIEM 420
           IFGDSFIFSN FIKDLYDRMEKEMETI VPGSSTGIFSGDS SSSKLGNDPSMS E IE 
Sbjct: 391 IFGDSFIFSNTFIKDLYDRMEKEMETITVPGSSTGIFSGDSQSSSKLGNDPSMSTESIET 450

Query: 421 GNDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQESSTKSKKNQRKAKATSNVQVA 480
           GNDSGKTG+I++K+SKKKKGKSIGNTQS AAEGALDDQESSTKSKKNQRK + TSNVQVA
Sbjct: 451 GNDSGKTGDIMDKKSKKKKGKSIGNTQSTAAEGALDDQESSTKSKKNQRKTRGTSNVQVA 510

Query: 481 ETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEEHGIDDPAIIVEPLVNHLRPML 540
           ETKAGGKKES KTKE+NINYPTE WVIEKIKTLIPDLEEHGIDDP IIV+PL NHLRPML
Sbjct: 511 ETKAGGKKESAKTKESNINYPTEEWVIEKIKTLIPDLEEHGIDDPTIIVQPLANHLRPML 570

Query: 541 NNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 600
           NN WRERRKALFTENAEKMKRLLD+TQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH
Sbjct: 571 NNLWRERRKALFTENAEKMKRLLDNTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 630

Query: 601 LLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGERITIAKSFPGSLSNMA 660
           LLRTTAAPIVDMLFHNLDLYNKLKNGIE+AELQ+SE+V+L+TGER TIAKSFPGSLSN A
Sbjct: 631 LLRTTAAPIVDMLFHNLDLYNKLKNGIEVAELQNSEAVALSTGERTTIAKSFPGSLSNKA 690

Query: 661 VTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSYRKDLTSQVSAEMDAI 720
           VTVAEALEGKRVETFINALGDLVEESGMI KKLDKKLERTLLHSYRK+LTSQVSAEMD I
Sbjct: 691 VTVAEALEGKRVETFINALGDLVEESGMIPKKLDKKLERTLLHSYRKELTSQVSAEMDPI 750

Query: 721 SLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKILSDYQTATVTLLSLIS 780
           +LLPKVVSLLY+QI+HKALQAPGRAIS AISRLKDKLDDSA+KILSDYQTATVTLLSLIS
Sbjct: 751 ALLPKVVSLLYVQIYHKALQAPGRAISVAISRLKDKLDDSAHKILSDYQTATVTLLSLIS 810

Query: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA 816
           AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA
Sbjct: 811 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA 845

BLAST of Clc03G01930 vs. ExPASy TrEMBL
Match: A0A1S3C5N8 (E3 UFM1-protein ligase 1 homolog OS=Cucumis melo OX=3656 GN=LOC103497188 PE=3 SV=1)

HSP 1 Score: 1446.8 bits (3744), Expect = 0.0e+00
Identity = 765/815 (93.87%), Postives = 790/815 (96.93%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRIL+FELLHTVTGKEYITPEH
Sbjct: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDS 120
           LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDP+LTLIQGEIISQSYWDS
Sbjct: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPQLTLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLEGGQLYTPAYVAR 180
           VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQR+GTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEILG 240
           VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVD SFFQSLFNGI+KE E+LG
Sbjct: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDASFFQSLFNGIMKENEVLG 240

Query: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300

Query: 301 TFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360
           TFIHPSIIEMLDS ++DILERGSW NSLLVLPSSFEPQDASK+LLSCPSVQ ALKSNKAL
Sbjct: 301 TFIHPSIIEMLDSAIEDILERGSWANSLLVLPSSFEPQDASKVLLSCPSVQGALKSNKAL 360

Query: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGNDPSMSAEPIEM 420
           IFGDSFIFSNIFIKDL+DRMEKEMETI VPGSSTGIFSGDS SSSKLG DPSMS E IEM
Sbjct: 361 IFGDSFIFSNIFIKDLFDRMEKEMETIIVPGSSTGIFSGDSQSSSKLGIDPSMSTESIEM 420

Query: 421 GNDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQESSTKSKKNQRKAKATSNVQVA 480
           GNDSGKTGEIVEK+SKKKKGKS+GNTQS  AEGALDDQESSTKSKKNQRK + TSNVQVA
Sbjct: 421 GNDSGKTGEIVEKKSKKKKGKSVGNTQSTTAEGALDDQESSTKSKKNQRKTRGTSNVQVA 480

Query: 481 ETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEEHGIDDPAIIVEPLVNHLRPML 540
           ETK GGKKESVKTKE+NINYPTE WVIEKIKTLIPDLEEHGIDDP IIV+PL NHLRPML
Sbjct: 481 ETKTGGKKESVKTKESNINYPTEEWVIEKIKTLIPDLEEHGIDDPTIIVQPLANHLRPML 540

Query: 541 NNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 600
           NN WRERRKALFTENAEKMK LLD+TQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH
Sbjct: 541 NNLWRERRKALFTENAEKMKHLLDNTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGERITIAKSFPGSLSNMA 660
           LLRTTAAPIVDMLFHNLDLYNKLKNGIE+AELQ+SE V+L+TGERITIAKSFPGSLSN A
Sbjct: 601 LLRTTAAPIVDMLFHNLDLYNKLKNGIEVAELQNSEPVALSTGERITIAKSFPGSLSNKA 660

Query: 661 VTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSYRKDLTSQVSAEMDAI 720
           VTVAEALEGKRVETFINALGDLVEESGMI KKLDKKLERTLLHSYRK+LTSQVS+EMD I
Sbjct: 661 VTVAEALEGKRVETFINALGDLVEESGMISKKLDKKLERTLLHSYRKELTSQVSSEMDPI 720

Query: 721 SLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKILSDYQTATVTLLSLIS 780
           +LLPKVVSLLY+QI+HKALQAPGRAIS AISRLKDKLDDSA+KILSDYQTATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYVQIYHKALQAPGRAISVAISRLKDKLDDSAHKILSDYQTATVTLLSLIS 780

Query: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA 816
           AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA
Sbjct: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA 815

BLAST of Clc03G01930 vs. ExPASy TrEMBL
Match: A0A6J1I5Q5 (E3 UFM1-protein ligase 1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111469466 PE=3 SV=1)

HSP 1 Score: 1414.4 bits (3660), Expect = 0.0e+00
Identity = 745/815 (91.41%), Postives = 783/815 (96.07%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEH 60
           MD ELLELQRQFEFAQQAKS+IRLSERNVVELVQKLQELRIL+FELLHTVTGKEYITPEH
Sbjct: 1   MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDS 120
           LRREILAEIEKLGRISLIDLAD IGVDLYYIEKQAEQI+SDDPRL LIQGEIISQSYWDS
Sbjct: 61  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLEGGQLYTPAYVAR 180
             EEINERLQESSQIALAEIAA+LQVGSELLASML QR+GTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEILG 240
           VSAMVRGATRA TVPTNLTVIWSSLQQLL GIDGASGIAVDGSFFQSLFNG+VKE E+LG
Sbjct: 181 VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 240

Query: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTP IFSIAQKE IDSFFSQNS+I+YEFLRKLGIPNPI FLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 300

Query: 301 TFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360
           TFIHPSIIEMLDSTV+DILERGSWTNSLLVLPSSFEPQDASKILLSCPSV+VALK NKA 
Sbjct: 301 TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 360

Query: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGNDPSMSAEPIEM 420
           IFGDSFIFSNIFIKDLYDRMEKEMET+NVPGSST  FSGDSLS SK+GNDPSMSAE IE 
Sbjct: 361 IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 420

Query: 421 GNDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQESSTKSKKNQRKAKATSNVQVA 480
           GN+SGK GEI+EK+SKKKKGKS GNTQ+IAAEGALDDQESSTKSKKNQRKAKAT++VQVA
Sbjct: 421 GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATASVQVA 480

Query: 481 ETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEEHGIDDPAIIVEPLVNHLRPML 540
           ETKAGGKKESVK KE+NINYPTE WVIEK+KTLIPDL+EHGIDDPA IV+PLVNHLRPML
Sbjct: 481 ETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPML 540

Query: 541 NNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 600
           NNSW+ERRKA FTENAEKMKRLLDSTQQKLDESF++LQLYEKALDLFEDDQSISV+LHRH
Sbjct: 541 NNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGERITIAKSFPGSLSNMA 660
           LLRT+AAPIVDMLFHNLDLYNKLKNGIE+AE+Q+SE VSL+TGER TIAKSFPGSLSN A
Sbjct: 601 LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA 660

Query: 661 VTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSYRKDLTSQVSAEMDAI 720
           VTVAEALEGKRVETF++ALGDLVEESGMI KKLDKKLERTLLHSYRKDLTSQVSAEMD I
Sbjct: 661 VTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720

Query: 721 SLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKILSDYQTATVTLLSLIS 780
           +LLPKVVSLLY+QIHHKALQAPGRAIS AISRLKDKLD+ AYKILSDYQ+ATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYVQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLIS 780

Query: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA 816
           AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA
Sbjct: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA 815

BLAST of Clc03G01930 vs. ExPASy TrEMBL
Match: A0A6J1GB94 (E3 UFM1-protein ligase 1 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452413 PE=3 SV=1)

HSP 1 Score: 1413.3 bits (3657), Expect = 0.0e+00
Identity = 747/815 (91.66%), Postives = 782/815 (95.95%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRIL+FELLHTVTGKEYITPEH
Sbjct: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDS 120
           LRREILAEIEKLGRISLIDLADTIGVDLYY+EKQAEQI+SDDPRL LIQGEIISQSYWDS
Sbjct: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYVEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLEGGQLYTPAYVAR 180
           VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQR+GTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEILG 240
           VSAMVRGATRAITVPTNLTVIWSSLQQLLQGI+GASGIAVD SFFQSLFNG+VKE EILG
Sbjct: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240

Query: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTPNIFSIAQKE +DSFFSQNS+ISYEFLRKLG+PNPIQFLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300

Query: 301 TFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360
           TFIHPSI+EMLDSTV+DILERGSWTNSLLVLPSSFEPQDASK+LLSCPSV+V+LKSN AL
Sbjct: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360

Query: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGNDPSMSAEPIEM 420
           IFGDSFIFSNIFIKDLY RMEKEMETINVPGSS G FSGDSLSSSK   DPSM +E IE 
Sbjct: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSSKFQTDPSMFSESIET 420

Query: 421 GNDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQESSTKSKKNQRKAKATSNVQVA 480
           GN+SG+TGEI+EK+SKKKKGKS GNTQS AAE ALDDQE STKSKKNQRKAKA+S+VQVA
Sbjct: 421 GNNSGRTGEIMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480

Query: 481 ETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEEHGIDDPAIIVEPLVNHLRPML 540
           ETKAGGKKESVKTKEN+I YPTE WVIEKIKTLIPDLEEHGIDDPAII++PLVNHLRPML
Sbjct: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540

Query: 541 NNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 600
           NNSWRERRKALF+ENAEKMKRLLD+TQQKLD SFLNLQLY KALDLFEDDQSISVILHRH
Sbjct: 541 NNSWRERRKALFSENAEKMKRLLDNTQQKLDGSFLNLQLYGKALDLFEDDQSISVILHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGERITIAKSFPGSLSNMA 660
           LLRTTA+PIVDMLFHNLDL+NKLKNGIE+AELQ+SE VSL+TGER TIAKSFPGSLSN A
Sbjct: 601 LLRTTASPIVDMLFHNLDLHNKLKNGIEVAELQNSEPVSLSTGERTTIAKSFPGSLSNKA 660

Query: 661 VTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSYRKDLTSQVSAEMDAI 720
           VTVAEALEGK ++TFINAL DL EESGMI KKLDKKLERTLLHSYRKDLTSQVSAEMD I
Sbjct: 661 VTVAEALEGKLMDTFINALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720

Query: 721 SLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKILSDYQTATVTLLSLIS 780
           +LLPKVVSLLYIQIHHKALQAPGRAIS AISRLKDKLDDSA+KILSDYQTATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780

Query: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA 816
           AA GDEDDCSSDRILTKREFLESQIPALKGLV SA
Sbjct: 781 AAAGDEDDCSSDRILTKREFLESQIPALKGLVFSA 815

BLAST of Clc03G01930 vs. ExPASy TrEMBL
Match: A0A6J1DBM9 (E3 UFM1-protein ligase 1 homolog OS=Momordica charantia OX=3673 GN=LOC111019510 PE=3 SV=1)

HSP 1 Score: 1412.1 bits (3654), Expect = 0.0e+00
Identity = 742/815 (91.04%), Postives = 785/815 (96.32%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRIL+F+LLHTV+GKEYITPEH
Sbjct: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFDLLHTVSGKEYITPEH 60

Query: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDS 120
           LRREILAEIEKLGRISLIDLADTIGVDLYY+EKQAEQI+SDDPRL LIQGEIISQSYWDS
Sbjct: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYVEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLEGGQLYTPAYVAR 180
           VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQR+GTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEILG 240
           VSAMVRGATR+ITVPTNLTVIWSSLQQLLQGI+GASGIAVDGSFFQSLFNG++KE E+LG
Sbjct: 181 VSAMVRGATRSITVPTNLTVIWSSLQQLLQGINGASGIAVDGSFFQSLFNGLMKENEVLG 240

Query: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTP IFSIAQKE IDSFFSQNSV+SYE+LRKLGIPNPIQFLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPTIFSIAQKECIDSFFSQNSVVSYEYLRKLGIPNPIQFLQSRYPDGIPLST 300

Query: 301 TFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360
           TFIHPSIIEMLDSTV+DILE GSWTNSLLVLPSSFEPQDASKIL SCPSVQVALKSNKAL
Sbjct: 301 TFIHPSIIEMLDSTVEDILECGSWTNSLLVLPSSFEPQDASKILSSCPSVQVALKSNKAL 360

Query: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGNDPSMSAEPIEM 420
           IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSS GIFS DSLSSSKLGNDPS  AE +E 
Sbjct: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSAGIFSADSLSSSKLGNDPSTFAESVET 420

Query: 421 GNDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQESSTKSKKNQRKAKATSNVQVA 480
           GNDSGKTGEI+EKRSKKKKGKS GNT S AAE A+DDQESSTKSKKNQRKAKATS+VQVA
Sbjct: 421 GNDSGKTGEIMEKRSKKKKGKSTGNTLSTAAESAVDDQESSTKSKKNQRKAKATSSVQVA 480

Query: 481 ETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEEHGIDDPAIIVEPLVNHLRPML 540
           E K GGKKESVK+KENNIN PTE WV EKIKT++PD EEHGIDDPAII++PLVNH+RPML
Sbjct: 481 EAKTGGKKESVKSKENNINIPTEEWVTEKIKTMMPDFEEHGIDDPAIILQPLVNHIRPML 540

Query: 541 NNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 600
           NNSWRERRKALFTENAEKMKR+LDSTQQKLDE+FLNLQLYEKALDLFEDDQSISVILHRH
Sbjct: 541 NNSWRERRKALFTENAEKMKRVLDSTQQKLDETFLNLQLYEKALDLFEDDQSISVILHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGERITIAKSFPGSLSNMA 660
           LLRT+AAPIVD+LFHNLDL+NKLKNGIE+AELQ+SESVSLNTGER T+AKS PG+LSN A
Sbjct: 601 LLRTSAAPIVDLLFHNLDLHNKLKNGIEVAELQNSESVSLNTGERTTLAKSLPGTLSNKA 660

Query: 661 VTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSYRKDLTSQVSAEMDAI 720
           +TVAEALEGKRVETF++ALGDLVEESGM+FKKLDKKLERTLLHSYRK+LTSQVSAEMD I
Sbjct: 661 LTVAEALEGKRVETFVSALGDLVEESGMVFKKLDKKLERTLLHSYRKELTSQVSAEMDPI 720

Query: 721 SLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKILSDYQTATVTLLSLIS 780
           +LLPKVVSLLY+QIHHKALQAPGRAIS AISRLKDKLDDSAYKIL+DYQ+ATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYLQIHHKALQAPGRAISVAISRLKDKLDDSAYKILTDYQSATVTLLSLIS 780

Query: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLSA 816
           AA GDE DCSSDRILTKRE LES+IPALKGLVLSA
Sbjct: 781 AATGDE-DCSSDRILTKRELLESRIPALKGLVLSA 814

BLAST of Clc03G01930 vs. TAIR 10
Match: AT3G46220.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2042 (InterPro:IPR018611); Has 559 Blast hits to 533 proteins in 154 species: Archae - 3; Bacteria - 32; Metazoa - 305; Fungi - 42; Plants - 50; Viruses - 3; Other Eukaryotes - 124 (source: NCBI BLink). )

HSP 1 Score: 969.5 bits (2505), Expect = 1.6e-282
Identity = 513/814 (63.02%), Postives = 646/814 (79.36%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQ KSS+RLS+RNVVELVQKLQEL +++F+LLHTVTGKEYIT E 
Sbjct: 1   MDDELLELQRQFEFAQQVKSSVRLSDRNVVELVQKLQELGVIDFDLLHTVTGKEYITQEQ 60

Query: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDS 120
           LR EI  EI KLGR+S+IDLADTIGVDLY++EKQA+ +V +DP L L+QGEIISQSYWDS
Sbjct: 61  LRNEITREISKLGRVSVIDLADTIGVDLYHVEKQAQDVVLNDPGLMLVQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLEGGQLYTPAYVAR 180
           +AEEINERLQE SQIA+AE+A +LQVGSEL+ S+L+ R+GTLVK RLEGGQLYTPAYV R
Sbjct: 121 IAEEINERLQECSQIAVAELAGQLQVGSELVQSVLEPRLGTLVKARLEGGQLYTPAYVER 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEILG 240
           V+AMVRGA+R I VP+NL+ +W+ LQQL+Q  +GASG+AV+ SFFQS+FN ++KE+E+LG
Sbjct: 181 VTAMVRGASRGIFVPSNLSALWAPLQQLVQETNGASGVAVENSFFQSIFNRLLKEEEMLG 240

Query: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300
           SLRAG HWTP+ F+ AQKE +DS FSQNS ISYE ++KLGI   +QFLQSRYPDG PL+ 
Sbjct: 241 SLRAGTHWTPSAFATAQKECVDSSFSQNSYISYESMQKLGISQAVQFLQSRYPDGTPLAA 300

Query: 301 TFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360
            FIH S+IEMLDS  +D +E+ SW +SL VLPSSF  QDA+K+LL CPSVQ ALK+ KAL
Sbjct: 301 VFIHSSMIEMLDSATEDAIEQNSWIDSLSVLPSSFTSQDANKMLLLCPSVQSALKAEKAL 360

Query: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGNDPSMSAEPIEM 420
           I G+S++ S+ FIK +YD++EKE +  ++  S+  +    S SS         S E I  
Sbjct: 361 ILGESYVLSSGFIKGIYDQIEKEADAFSIQASTATLIHPSSKSSE--------STESIPA 420

Query: 421 GNDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQESS-TKSKKNQRKAKATSNVQV 480
             D G         SKKKKGKS  +T++   E   DD+E +  KSK+NQ+K + +S+ Q 
Sbjct: 421 NTDKG---------SKKKKGKS-ASTKAATVETVPDDEEDARPKSKRNQKKGRDSSSSQK 480

Query: 481 AETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEEHGIDDPAIIVEPLVNHLRPM 540
            ++KAGGKKESVK +E+N   P + WV++KI   +P+ E+ G ++P  I++ L +H++PM
Sbjct: 481 LDSKAGGKKESVKAQESNNIIPPDEWVMKKIVDSVPEFEDDGTENPDSILKHLADHMKPM 540

Query: 541 LNNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNLQLYEKALDLFEDDQSISVILHR 600
           L NS +ERRK +FTENA++M+RL+D  Q+KLDESFLN+QLYEKALDLFEDDQS +V+LHR
Sbjct: 541 LINSLKERRKKIFTENADRMRRLIDDLQKKLDESFLNMQLYEKALDLFEDDQSTAVVLHR 600

Query: 601 HLLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGERITIAKSFPGSLSNM 660
           HLLRTTAA I D L H LD++NK+KNG E+ E ++ + V L++ ER  +AK+  GSLS  
Sbjct: 601 HLLRTTAATIADTLLHGLDIHNKMKNGTEVEESKTQDLVLLDSSERTALAKNLNGSLSKK 660

Query: 661 AVTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSYRKDLTSQVSAEMDA 720
           A+ + EALEGKRV+TF+    DL EESG++ KKLDKKLERTLLHSYRKDL SQVS E D 
Sbjct: 661 ALALVEALEGKRVDTFMVTFRDLAEESGLVLKKLDKKLERTLLHSYRKDLISQVSTESDP 720

Query: 721 ISLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKILSDYQTATVTLLSLI 780
           I+LL KVVSLL+I+IH+KALQAPGRAI+AAIS LK+KLD+SAYK L+DYQTATVTLL+L+
Sbjct: 721 IALLAKVVSLLFIKIHNKALQAPGRAIAAAISHLKEKLDESAYKTLTDYQTATVTLLALM 780

Query: 781 SAAVGDEDDCSSDRILTKREFLESQIPALKGLVL 814
           SA+ G+E DCS+DRILTKRE LESQ+P L+ LVL
Sbjct: 781 SASSGEEHDCSADRILTKRELLESQMPLLRTLVL 796

BLAST of Clc03G01930 vs. TAIR 10
Match: AT3G46220.2 (unknown protein; INVOLVED IN: biological_process unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2042 (InterPro:IPR018611). )

HSP 1 Score: 962.2 bits (2486), Expect = 2.6e-280
Identity = 513/822 (62.41%), Postives = 646/822 (78.59%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQ KSS+RLS+RNVVELVQKLQEL +++F+LLHTVTGKEYIT E 
Sbjct: 1   MDDELLELQRQFEFAQQVKSSVRLSDRNVVELVQKLQELGVIDFDLLHTVTGKEYITQEQ 60

Query: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDS 120
           LR EI  EI KLGR+S+IDLADTIGVDLY++EKQA+ +V +DP L L+QGEIISQSYWDS
Sbjct: 61  LRNEITREISKLGRVSVIDLADTIGVDLYHVEKQAQDVVLNDPGLMLVQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLEGGQLYTPAYVAR 180
           +AEEINERLQE SQIA+AE+A +LQVGSEL+ S+L+ R+GTLVK RLEGGQLYTPAYV R
Sbjct: 121 IAEEINERLQECSQIAVAELAGQLQVGSELVQSVLEPRLGTLVKARLEGGQLYTPAYVER 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEILG 240
           V+AMVRGA+R I VP+NL+ +W+ LQQL+Q  +GASG+AV+ SFFQS+FN ++KE+E+LG
Sbjct: 181 VTAMVRGASRGIFVPSNLSALWAPLQQLVQETNGASGVAVENSFFQSIFNRLLKEEEMLG 240

Query: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300
           SLRAG HWTP+ F+ AQKE +DS FSQNS ISYE ++KLGI   +QFLQSRYPDG PL+ 
Sbjct: 241 SLRAGTHWTPSAFATAQKECVDSSFSQNSYISYESMQKLGISQAVQFLQSRYPDGTPLAA 300

Query: 301 TFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360
            FIH S+IEMLDS  +D +E+ SW +SL VLPSSF  QDA+K+LL CPSVQ ALK+ KAL
Sbjct: 301 VFIHSSMIEMLDSATEDAIEQNSWIDSLSVLPSSFTSQDANKMLLLCPSVQSALKAEKAL 360

Query: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGNDPSMSAEPIEM 420
           I G+S++ S+ FIK +YD++EKE +  ++  S+  +    S SS         S E I  
Sbjct: 361 ILGESYVLSSGFIKGIYDQIEKEADAFSIQASTATLIHPSSKSSE--------STESIPA 420

Query: 421 GNDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQESS-TKSKKNQRKAKATSNVQV 480
             D G         SKKKKGKS  +T++   E   DD+E +  KSK+NQ+K + +S+ Q 
Sbjct: 421 NTDKG---------SKKKKGKS-ASTKAATVETVPDDEEDARPKSKRNQKKGRDSSSSQK 480

Query: 481 AETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEEHG--------IDDPAIIVEP 540
            ++KAGGKKESVK +E+N   P + WV++KI   +P+ E+ G         ++P  I++ 
Sbjct: 481 LDSKAGGKKESVKAQESNNIIPPDEWVMKKIVDSVPEFEDDGTSENFFLCTENPDSILKH 540

Query: 541 LVNHLRPMLNNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNLQLYEKALDLFEDDQ 600
           L +H++PML NS +ERRK +FTENA++M+RL+D  Q+KLDESFLN+QLYEKALDLFEDDQ
Sbjct: 541 LADHMKPMLINSLKERRKKIFTENADRMRRLIDDLQKKLDESFLNMQLYEKALDLFEDDQ 600

Query: 601 SISVILHRHLLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGERITIAKS 660
           S +V+LHRHLLRTTAA I D L H LD++NK+KNG E+ E ++ + V L++ ER  +AK+
Sbjct: 601 STAVVLHRHLLRTTAATIADTLLHGLDIHNKMKNGTEVEESKTQDLVLLDSSERTALAKN 660

Query: 661 FPGSLSNMAVTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSYRKDLTS 720
             GSLS  A+ + EALEGKRV+TF+    DL EESG++ KKLDKKLERTLLHSYRKDL S
Sbjct: 661 LNGSLSKKALALVEALEGKRVDTFMVTFRDLAEESGLVLKKLDKKLERTLLHSYRKDLIS 720

Query: 721 QVSAEMDAISLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKILSDYQTA 780
           QVS E D I+LL KVVSLL+I+IH+KALQAPGRAI+AAIS LK+KLD+SAYK L+DYQTA
Sbjct: 721 QVSTESDPIALLAKVVSLLFIKIHNKALQAPGRAIAAAISHLKEKLDESAYKTLTDYQTA 780

Query: 781 TVTLLSLISAAVGDEDDCSSDRILTKREFLESQIPALKGLVL 814
           TVTLL+L+SA+ G+E DCS+DRILTKRE LESQ+P L+ LVL
Sbjct: 781 TVTLLALMSASSGEEHDCSADRILTKRELLESQMPLLRTLVL 804

BLAST of Clc03G01930 vs. TAIR 10
Match: AT3G46220.3 (unknown protein; INVOLVED IN: biological_process unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2042 (InterPro:IPR018611). )

HSP 1 Score: 940.3 bits (2429), Expect = 1.1e-273
Identity = 503/814 (61.79%), Postives = 634/814 (77.89%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILEFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQ KSS+RLS+RNVVELVQKLQEL +++F+LLHTVTGKEYIT E 
Sbjct: 1   MDDELLELQRQFEFAQQVKSSVRLSDRNVVELVQKLQELGVIDFDLLHTVTGKEYITQEQ 60

Query: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPRLTLIQGEIISQSYWDS 120
           LR EI  EI KLGR+S+IDLADTIGVDLY++EKQA+ +V +DP L L+QGEIISQSYWDS
Sbjct: 61  LRNEITREISKLGRVSVIDLADTIGVDLYHVEKQAQDVVLNDPGLMLVQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRIGTLVKGRLEGGQLYTPAYVAR 180
           +AEEINERLQE SQIA+AE+A +LQVGSEL+ S+L+ R+GTLVK RLEGGQLYTPAYV R
Sbjct: 121 IAEEINERLQECSQIAVAELAGQLQVGSELVQSVLEPRLGTLVKARLEGGQLYTPAYVER 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDGSFFQSLFNGIVKEKEILG 240
           V+AMVRGA+R I VP+NL+ +W+ LQQL+Q  +GASG+AV+ SFFQS+FN ++KE+E+LG
Sbjct: 181 VTAMVRGASRGIFVPSNLSALWAPLQQLVQETNGASGVAVENSFFQSIFNRLLKEEEMLG 240

Query: 241 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300
           SLRAG HWTP+                NS ISYE ++KLGI   +QFLQSRYPDG PL+ 
Sbjct: 241 SLRAGTHWTPS----------------NSYISYESMQKLGISQAVQFLQSRYPDGTPLAA 300

Query: 301 TFIHPSIIEMLDSTVDDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360
            FIH S+IEMLDS  +D +E+ SW +SL VLPSSF  QDA+K+LL CPSVQ ALK+ KAL
Sbjct: 301 VFIHSSMIEMLDSATEDAIEQNSWIDSLSVLPSSFTSQDANKMLLLCPSVQSALKAEKAL 360

Query: 361 IFGDSFIFSNIFIKDLYDRMEKEMETINVPGSSTGIFSGDSLSSSKLGNDPSMSAEPIEM 420
           I G+S++ S+ FIK +YD++EKE +  ++  S+  +    S SS         S E I  
Sbjct: 361 ILGESYVLSSGFIKGIYDQIEKEADAFSIQASTATLIHPSSKSSE--------STESIPA 420

Query: 421 GNDSGKTGEIVEKRSKKKKGKSIGNTQSIAAEGALDDQESS-TKSKKNQRKAKATSNVQV 480
             D G         SKKKKGKS  +T++   E   DD+E +  KSK+NQ+K + +S+ Q 
Sbjct: 421 NTDKG---------SKKKKGKS-ASTKAATVETVPDDEEDARPKSKRNQKKGRDSSSSQK 480

Query: 481 AETKAGGKKESVKTKENNINYPTEAWVIEKIKTLIPDLEEHGIDDPAIIVEPLVNHLRPM 540
            ++KAGGKKESVK +E+N   P + WV++KI   +P+ E+ G ++P  I++ L +H++PM
Sbjct: 481 LDSKAGGKKESVKAQESNNIIPPDEWVMKKIVDSVPEFEDDGTENPDSILKHLADHMKPM 540

Query: 541 LNNSWRERRKALFTENAEKMKRLLDSTQQKLDESFLNLQLYEKALDLFEDDQSISVILHR 600
           L NS +ERRK +FTENA++M+RL+D  Q+KLDESFLN+QLYEKALDLFEDDQS +V+LHR
Sbjct: 541 LINSLKERRKKIFTENADRMRRLIDDLQKKLDESFLNMQLYEKALDLFEDDQSTAVVLHR 600

Query: 601 HLLRTTAAPIVDMLFHNLDLYNKLKNGIEIAELQSSESVSLNTGERITIAKSFPGSLSNM 660
           HLLRTTAA I D L H LD++NK+KNG E+ E ++ + V L++ ER  +AK+  GSLS  
Sbjct: 601 HLLRTTAATIADTLLHGLDIHNKMKNGTEVEESKTQDLVLLDSSERTALAKNLNGSLSKK 660

Query: 661 AVTVAEALEGKRVETFINALGDLVEESGMIFKKLDKKLERTLLHSYRKDLTSQVSAEMDA 720
           A+ + EALEGKRV+TF+    DL EESG++ KKLDKKLERTLLHSYRKDL SQVS E D 
Sbjct: 661 ALALVEALEGKRVDTFMVTFRDLAEESGLVLKKLDKKLERTLLHSYRKDLISQVSTESDP 720

Query: 721 ISLLPKVVSLLYIQIHHKALQAPGRAISAAISRLKDKLDDSAYKILSDYQTATVTLLSLI 780
           I+LL KVVSLL+I+IH+KALQAPGRAI+AAIS LK+KLD+SAYK L+DYQTATVTLL+L+
Sbjct: 721 IALLAKVVSLLFIKIHNKALQAPGRAIAAAISHLKEKLDESAYKTLTDYQTATVTLLALM 780

Query: 781 SAAVGDEDDCSSDRILTKREFLESQIPALKGLVL 814
           SA+ G+E DCS+DRILTKRE LESQ+P L+ LVL
Sbjct: 781 SASSGEEHDCSADRILTKRELLESQMPLLRTLVL 780