Homology
BLAST of Clc03G00730 vs. NCBI nr
Match:
KAA0031835.1 (acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum [Cucumis melo var. makuwa])
HSP 1 Score: 774.2 bits (1998), Expect = 5.0e-220
Identity = 360/377 (95.49%), Postives = 372/377 (98.67%), Query Frame = 0
Query: 1 MAIPLPASGTGNGNGNATDAAPAPFDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPLPA+G GNGNGNAT AAP PFDPSAPPPFR+AEIRAAIPPHCWVKNPWRSLVYVLR
Sbjct: 1 MAIPLPANGNGNGNGNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR 60
Query: 61 DLAIVSALVAAAVFFDSWLFWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLI 120
DLA+VSALVAAAVFFDSWLFWPIYW+AQGTMFWAIFVLGHDCGHGSFSNSSALN+FMGL+
Sbjct: 61 DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA 180
LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLE+RTRILRFTLPFPILA
Sbjct: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFK 240
YPFYLMWRSPGKEGSHFNPYSDLFAP+ERKDIVISTSCWTLMAV+LVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
Query: 241 IYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI 300
+YGVPYWIFVMWLD VTYLHHHGYEQKLPWYRGEEW+YLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLV NLVNSL+QDHYVS
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS 360
Query: 361 DQGNIVFYQTDPYLYHY 378
DQGNIVFYQTDPYLYHY
Sbjct: 361 DQGNIVFYQTDPYLYHY 377
BLAST of Clc03G00730 vs. NCBI nr
Match:
XP_008457364.1 (PREDICTED: acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum [Cucumis melo])
HSP 1 Score: 765.0 bits (1974), Expect = 3.0e-217
Identity = 358/377 (94.96%), Postives = 370/377 (98.14%), Query Frame = 0
Query: 1 MAIPLPASGTGNGNGNATDAAPAPFDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPLPA+ GNGN NAT AAP PFDPSAPPPFR+AEIRAAIPPHCWVKNPWRSLVYVLR
Sbjct: 1 MAIPLPAN--GNGNSNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR 60
Query: 61 DLAIVSALVAAAVFFDSWLFWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLI 120
DLA+VSALVAAAVFFDSWLFWPIYW+AQGTMFWAIFVLGHDCGHGSFSNSSALN+FMGL+
Sbjct: 61 DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA 180
LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLE+RTRILRFTLPFPILA
Sbjct: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFK 240
YPFYLMWRSPGKEGSHFNPYSDLFAP+ERKDIVISTSCWTLMAV+LVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
Query: 241 IYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI 300
+YGVPYWIFVMWLD VTYLHHHGYEQKLPWYRGEEW+YLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLV NLVNSL+QDHYVS
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS 360
Query: 361 DQGNIVFYQTDPYLYHY 378
DQGNIVFYQTDPYLYHY
Sbjct: 361 DQGNIVFYQTDPYLYHY 375
BLAST of Clc03G00730 vs. NCBI nr
Match:
XP_038893495.1 (LOW QUALITY PROTEIN: omega-3 fatty acid desaturase, endoplasmic reticulum-like [Benincasa hispida])
HSP 1 Score: 745.0 bits (1922), Expect = 3.3e-211
Identity = 355/377 (94.16%), Postives = 360/377 (95.49%), Query Frame = 0
Query: 1 MAIPLPASGTGNGNGNATDAAPAPFDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPLPASGT GNGNAT A PAPFDPSAPPPFRM +IRAAIPPHCWVKNPWRSLVYVLR
Sbjct: 1 MAIPLPASGT--GNGNATAATPAPFDPSAPPPFRMXDIRAAIPPHCWVKNPWRSLVYVLR 60
Query: 61 DLAIVSALVAAAVFFDSWLFWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLI 120
DL IVSALV AVFFDSWLFWPIY VAQGTMFWA FVLGHDCGHGSFSNS ALN+FMGL+
Sbjct: 61 DLVIVSALVGRAVFFDSWLFWPIYLVAQGTMFWAXFVLGHDCGHGSFSNSYALNSFMGLL 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA 180
LHSFILVP RISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA
Sbjct: 121 LHSFILVPDQELRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFK 240
YPFYLMWRSPGKEGSHFNPYSDLFAPNERKDIVISTSCWTLMAV+LVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
Query: 241 IYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI 300
IYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 IYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP HLV NLVNSLRQDHYV+
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPFHLVNNLVNSLRQDHYVN 360
Query: 361 DQGNIVFYQTDPYLYHY 378
DQGNIVFYQTDPYLYHY
Sbjct: 361 DQGNIVFYQTDPYLYHY 375
BLAST of Clc03G00730 vs. NCBI nr
Match:
XP_004145254.1 (acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum [Cucumis sativus] >KGN65813.1 hypothetical protein Csa_023305 [Cucumis sativus])
HSP 1 Score: 745.0 bits (1922), Expect = 3.3e-211
Identity = 350/377 (92.84%), Postives = 363/377 (96.29%), Query Frame = 0
Query: 1 MAIPLPASGTGNGNGNATDAAPAPFDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPL A N NAT AAPAPFDPSAPPPFR++EIRAAIPPHCWVK+PWRSL+YVLR
Sbjct: 1 MAIPLLA------NANATAAAPAPFDPSAPPPFRISEIRAAIPPHCWVKSPWRSLLYVLR 60
Query: 61 DLAIVSALVAAAVFFDSWLFWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLI 120
DLAIVSALVAAA+FFDSWL WPIYW+AQGTMFWAIFVLGHDCGHGSFSNSSALN+FMGL+
Sbjct: 61 DLAIVSALVAAALFFDSWLVWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA 180
LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA
Sbjct: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFK 240
YPFYLMWRSPGKEGSHFNPYSDLFAP ER+DIVISTSCWTLMA +LVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPGERRDIVISTSCWTLMAALLVYLSFVFGPFQIFK 240
Query: 241 IYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI 300
+YGVPYWIFVMWLD VTYLHHHGYEQKLPWYRGEEW+YLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP HLVKNLVNSL+QDHYVS
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPTHLVKNLVNSLKQDHYVS 360
Query: 361 DQGNIVFYQTDPYLYHY 378
DQGNIVFYQTDPYLYHY
Sbjct: 361 DQGNIVFYQTDPYLYHY 371
BLAST of Clc03G00730 vs. NCBI nr
Match:
XP_022970609.1 (omega-3 fatty acid desaturase, endoplasmic reticulum-like [Cucurbita maxima])
HSP 1 Score: 732.6 bits (1890), Expect = 1.7e-207
Identity = 342/377 (90.72%), Postives = 359/377 (95.23%), Query Frame = 0
Query: 1 MAIPLPASGTGNGNGNATDAAPAPFDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPLPAS T NG AA APFDPSAPPPFR+AEIRAAIPPHCWVK+PWRSL+YVLR
Sbjct: 1 MAIPLPASATATANGT---AALAPFDPSAPPPFRIAEIRAAIPPHCWVKSPWRSLLYVLR 60
Query: 61 DLAIVSALVAAAVFFDSWLFWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLI 120
DLA+VSAL+AAAVFFDSWL WPIYWVAQGTMFWAIFVLGHDCGHGSFSNS+ALN +GL+
Sbjct: 61 DLAVVSALIAAAVFFDSWLLWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSTALNTLVGLV 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA 180
LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLE+RTRILRFTLPFPILA
Sbjct: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFK 240
YPFYLMWRSPGK+GSHFNP SDLFAPNERKDIVISTSCW LMA +LVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKKGSHFNPNSDLFAPNERKDIVISTSCWALMAAVLVYLSFVFGPFQIFK 240
Query: 241 IYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI 300
IYGVPYWIFVMWLD VTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 IYGVPYWIFVMWLDLVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHL++NLVNS R+DH+VS
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLMENLVNSFREDHFVS 360
Query: 361 DQGNIVFYQTDPYLYHY 378
D GNIVFYQTDPYL+H+
Sbjct: 361 DNGNIVFYQTDPYLHHH 374
BLAST of Clc03G00730 vs. ExPASy Swiss-Prot
Match:
P48626 (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Nicotiana tabacum OX=4097 GN=FAD3 PE=2 SV=1)
HSP 1 Score: 588.2 bits (1515), Expect = 6.7e-167
Identity = 267/351 (76.07%), Postives = 301/351 (85.75%), Query Frame = 0
Query: 25 FDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLRDLAIVSALVAAAVFFDSWLFWPIY 84
FDPSAPPPFR+AEIR IP HCWVK+P RSL YV+RD+ V+ L+ A+ DSWLF+P+Y
Sbjct: 22 FDPSAPPPFRLAEIRNVIPKHCWVKDPLRSLSYVVRDVIFVATLIGIAIHLDSWLFYPLY 81
Query: 85 WVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLILHSFILVPYHGWRISHRTHHQNHG 144
W QGTMFWAIFVLGHDCGHGSFS+S LNN +G ILHS ILVPYHGWRISH+THHQNHG
Sbjct: 82 WAIQGTMFWAIFVLGHDCGHGSFSDSQLLNNVVGHILHSAILVPYHGWRISHKTHHQNHG 141
Query: 145 NVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSDLF 204
NVE DESWVP+ +K Y ++ T+ LR+ +PFP+LAYP YLM RSPGK GSHFNPYSDLF
Sbjct: 142 NVETDESWVPMPEKLYNKVGYSTKFLRYKIPFPLLAYPMYLMKRSPGKSGSHFNPYSDLF 201
Query: 205 APNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFKIYGVPYWIFVMWLDFVTYLHHHGY 264
P+ERK +V ST CWT+MA +L+YL FG Q+FKIYG PY IFVMWLDFVTYLHHHGY
Sbjct: 202 QPHERKYVVTSTLCWTVMAALLLYLCTAFGSLQMFKIYGAPYLIFVMWLDFVTYLHHHGY 261
Query: 265 EQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAA 324
E+KLPWYRG+EWSYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHL EATKAA
Sbjct: 262 EKKLPWYRGKEWSYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLREATKAA 321
Query: 325 KGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVSDQGNIVFYQTDPYLY 376
K VLGKYYREPKKSGPIP HLVK+L S++QDHYVSD G IVFYQTDP+++
Sbjct: 322 KPVLGKYYREPKKSGPIPFHLVKDLTRSMKQDHYVSDSGEIVFYQTDPHIF 372
BLAST of Clc03G00730 vs. ExPASy Swiss-Prot
Match:
P48625 (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Glycine max OX=3847 GN=FAD3 PE=2 SV=1)
HSP 1 Score: 582.4 bits (1500), Expect = 3.7e-165
Identity = 262/363 (72.18%), Postives = 305/363 (84.02%), Query Frame = 0
Query: 14 NGNATDAAPAPFDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLRDLAIVSALVAAAV 73
NG + FDPSAPPPF++AEIRA+IP HCWVKNPWRSL YVLRD+ +++ALVAAA+
Sbjct: 14 NGYQQKGSSFDFDPSAPPPFKIAEIRASIPKHCWVKNPWRSLSYVLRDVLVIAALVAAAI 73
Query: 74 FFDSWLFWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLILHSFILVPYHGWR 133
FD+WL W IY QGTMFWA+FVLGHDCGHGSFS+S LN+ +G ILHS ILVPYHGWR
Sbjct: 74 HFDNWLLWLIYCPIQGTMFWALFVLGHDCGHGSFSDSPLLNSLVGHILHSSILVPYHGWR 133
Query: 134 ISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILAYPFYLMWRSPGKE 193
ISHRTHHQNHG++EKDESWVPLT+K YK L+ TR++RFT+PFP+ YP YL RSPGKE
Sbjct: 134 ISHRTHHQNHGHIEKDESWVPLTEKIYKNLDSMTRLIRFTVPFPLFVYPIYLFSRSPGKE 193
Query: 194 GSHFNPYSDLFAPNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFKIYGVPYWIFVMWL 253
GSHFNPYS+LF P+ERK I IST CW M +L+YLSF+ P + K+YG+PYWIFVMWL
Sbjct: 194 GSHFNPYSNLFPPSERKGIAISTLCWATMFSLLIYLSFITSPLLVLKLYGIPYWIFVMWL 253
Query: 254 DFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIP 313
DFVTYLHHHG+ QKLPWYRG+EWSYLRGGLTTVDRDYG NIHHDIGTHVIHHLFPQIP
Sbjct: 254 DFVTYLHHHGHHQKLPWYRGKEWSYLRGGLTTVDRDYGWIYNIHHDIGTHVIHHLFPQIP 313
Query: 314 HYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVSDQGNIVFYQTDPY 373
HYHLVEAT+AAK VLG YYREP++S P+P HL+K L+ S+RQDH+VSD G++V+YQTD
Sbjct: 314 HYHLVEATQAAKPVLGDYYREPERSAPLPFHLIKYLIQSMRQDHFVSDTGDVVYYQTDSL 373
Query: 374 LYH 377
L H
Sbjct: 374 LLH 376
BLAST of Clc03G00730 vs. ExPASy Swiss-Prot
Match:
P32291 (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Vigna radiata var. radiata OX=3916 GN=ARG1 PE=2 SV=1)
HSP 1 Score: 581.6 bits (1498), Expect = 6.2e-165
Identity = 266/363 (73.28%), Postives = 310/363 (85.40%), Query Frame = 0
Query: 13 GNGNATDAAPAPFDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLRDLAIVSALVAAA 72
GNG A + + FDP APPPF++A+IRAAIP HCW K+ RSL YVLRD+ +V+AL A+A
Sbjct: 11 GNG-AREGDQSYFDPGAPPPFKIADIRAAIPKHCWEKSTLRSLSYVLRDVLVVTALAASA 70
Query: 73 VFFDSWLFWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLILHSFILVPYHGW 132
+ F+SW FWP+YW AQGTMFWA+FVLGHDCGHGSFSNSS LN+F+G ILHS ILVPY+GW
Sbjct: 71 ISFNSWFFWPLYWPAQGTMFWALFVLGHDCGHGSFSNSSKLNSFVGHILHSLILVPYNGW 130
Query: 133 RISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILAYPFYLMWRSPGK 192
RISHRTHHQNHG+VEKDESWVPLT+K YK L+ TR+LR++ PFPI AYPFYL RSPGK
Sbjct: 131 RISHRTHHQNHGHVEKDESWVPLTEKVYKNLDDMTRMLRYSFPFPIFAYPFYLWNRSPGK 190
Query: 193 EGSHFNPYSDLFAPNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFKIYGVPYWIFVMW 252
EGSHFNPYS+LF+P ERK +V ST CW ++ +L+YLS GP + K+YGVPY IFVMW
Sbjct: 191 EGSHFNPYSNLFSPGERKGVVTSTLCWGIVLSVLLYLSLTIGPIFMLKLYGVPYLIFVMW 250
Query: 253 LDFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQI 312
LDFVTYLHHHGY KLPWYRG+EWSYLRGGLTTVDRDYG NN+HHDIGTHVIHHLFPQI
Sbjct: 251 LDFVTYLHHHGYTHKLPWYRGQEWSYLRGGLTTVDRDYGWINNVHHDIGTHVIHHLFPQI 310
Query: 313 PHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVSDQGNIVFYQTDP 372
PHYHLVEATK+AK VLGKYYREP+KSGP+P HL+K L+ S+ QDH+VSD G+IV+YQTDP
Sbjct: 311 PHYHLVEATKSAKSVLGKYYREPQKSGPLPFHLLKYLLQSISQDHFVSDTGDIVYYQTDP 370
Query: 373 YLY 376
L+
Sbjct: 371 KLH 372
BLAST of Clc03G00730 vs. ExPASy Swiss-Prot
Match:
P48622 (Temperature-sensitive sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD8 PE=1 SV=1)
HSP 1 Score: 581.3 bits (1497), Expect = 8.2e-165
Identity = 256/350 (73.14%), Postives = 301/350 (86.00%), Query Frame = 0
Query: 25 FDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLRDLAIVSALVAAAVFFDSWLFWPIY 84
FDP APPPF +A+IRAAIP HCWVKNPW S+ YV+RD+AIV L A A +F++WL WP+Y
Sbjct: 81 FDPGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLY 140
Query: 85 WVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLILHSFILVPYHGWRISHRTHHQNHG 144
W AQGTMFWA+FVLGHDCGHGSFSN LN+ G +LHS ILVPYHGWRISHRTHHQNHG
Sbjct: 141 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 200
Query: 145 NVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSDLF 204
+VE DESW PL + YK LEK T++ RFTLPFP+LAYPFYL RSPGK+GSH++P SDLF
Sbjct: 201 HVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLF 260
Query: 205 APNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFKIYGVPYWIFVMWLDFVTYLHHHGY 264
P E+KD++ ST+CWT MA +LV L+FV GP Q+ K+YG+PYWIFVMWLDFVTYLHHHG+
Sbjct: 261 LPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHGH 320
Query: 265 EQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAA 324
E KLPWYRG+EWSYLRGGLTT+DRDYG NNIHHDIGTHVIHHLFPQIPHYHLVEAT+AA
Sbjct: 321 EDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLVEATEAA 380
Query: 325 KGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVSDQGNIVFYQTDPYL 375
K VLGKYYREPK SGP+P+HL+ +L+ S++QDH+VSD G++V+Y+ DP L
Sbjct: 381 KPVLGKYYREPKNSGPLPLHLLGSLIKSMKQDHFVSDTGDVVYYEADPKL 430
BLAST of Clc03G00730 vs. ExPASy Swiss-Prot
Match:
P48624 (Acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum OS=Brassica napus OX=3708 GN=FAD3 PE=1 SV=1)
HSP 1 Score: 577.4 bits (1487), Expect = 1.2e-163
Identity = 265/366 (72.40%), Postives = 310/366 (84.70%), Query Frame = 0
Query: 12 NGNGNATDAAPAPFDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLRDLAIVSALVAA 71
N NG++ FDPSA PPF++ +IRAAIP HCWVK+P RS+ YV RD+ V+AL A
Sbjct: 10 NVNGDSGARKEEGFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVTRDIFAVAALAMA 69
Query: 72 AVFFDSWLFWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLILHSFILVPYHG 131
AV+FDSW WP+YWVAQGT+FWAIFVLGHDCGHGSFS+ LN+ +G ILHSFILVPYHG
Sbjct: 70 AVYFDSWFLWPLYWVAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFILVPYHG 129
Query: 132 WRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILAYPFYLMWRSPG 191
WRISHRTHHQNHG+VE DESWVPL +K YK L TR+LR+T+P P+LAYP YL +RSPG
Sbjct: 130 WRISHRTHHQNHGHVENDESWVPLPEKLYKNLPHSTRMLRYTVPLPMLAYPIYLWYRSPG 189
Query: 192 KEGSHFNPYSDLFAPNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFKIYGVPYWIFVM 251
KEGSHFNPYS LFAP+ERK I ST+CW++M LVYLSF+ P + K+YGVPY IFVM
Sbjct: 190 KEGSHFNPYSSLFAPSERKLIATSTTCWSIMLATLVYLSFLVDPVTVLKVYGVPYIIFVM 249
Query: 252 WLDFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQ 311
WLD VTYLHHHG+++KLPWYRG+EWSYLRGGLTT+DRDYG+FNNIHHDIGTHVIHHLFPQ
Sbjct: 250 WLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIFNNIHHDIGTHVIHHLFPQ 309
Query: 312 IPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVSDQGNIVFYQTD 371
IPHYHLV+AT+AAK VLG+YYREPK SG IPIHLV++LV S+++DHYVSD G+IVFY+TD
Sbjct: 310 IPHYHLVDATRAAKHVLGRYYREPKTSGAIPIHLVESLVASIKKDHYVSDTGDIVFYETD 369
Query: 372 PYLYHY 378
P LY Y
Sbjct: 370 PDLYVY 375
BLAST of Clc03G00730 vs. ExPASy TrEMBL
Match:
A0A5A7SRR2 (Acyl-lipid omega-3 desaturase (Cytochrome b5), endoplasmic reticulum OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold848G00850 PE=3 SV=1)
HSP 1 Score: 774.2 bits (1998), Expect = 2.4e-220
Identity = 360/377 (95.49%), Postives = 372/377 (98.67%), Query Frame = 0
Query: 1 MAIPLPASGTGNGNGNATDAAPAPFDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPLPA+G GNGNGNAT AAP PFDPSAPPPFR+AEIRAAIPPHCWVKNPWRSLVYVLR
Sbjct: 1 MAIPLPANGNGNGNGNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR 60
Query: 61 DLAIVSALVAAAVFFDSWLFWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLI 120
DLA+VSALVAAAVFFDSWLFWPIYW+AQGTMFWAIFVLGHDCGHGSFSNSSALN+FMGL+
Sbjct: 61 DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA 180
LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLE+RTRILRFTLPFPILA
Sbjct: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFK 240
YPFYLMWRSPGKEGSHFNPYSDLFAP+ERKDIVISTSCWTLMAV+LVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
Query: 241 IYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI 300
+YGVPYWIFVMWLD VTYLHHHGYEQKLPWYRGEEW+YLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLV NLVNSL+QDHYVS
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS 360
Query: 361 DQGNIVFYQTDPYLYHY 378
DQGNIVFYQTDPYLYHY
Sbjct: 361 DQGNIVFYQTDPYLYHY 377
BLAST of Clc03G00730 vs. ExPASy TrEMBL
Match:
A0A1S3C608 (acyl-lipid omega-3 desaturase (Cytochrome b5), endoplasmic reticulum OS=Cucumis melo OX=3656 GN=LOC103497071 PE=3 SV=1)
HSP 1 Score: 765.0 bits (1974), Expect = 1.5e-217
Identity = 358/377 (94.96%), Postives = 370/377 (98.14%), Query Frame = 0
Query: 1 MAIPLPASGTGNGNGNATDAAPAPFDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPLPA+ GNGN NAT AAP PFDPSAPPPFR+AEIRAAIPPHCWVKNPWRSLVYVLR
Sbjct: 1 MAIPLPAN--GNGNSNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR 60
Query: 61 DLAIVSALVAAAVFFDSWLFWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLI 120
DLA+VSALVAAAVFFDSWLFWPIYW+AQGTMFWAIFVLGHDCGHGSFSNSSALN+FMGL+
Sbjct: 61 DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA 180
LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLE+RTRILRFTLPFPILA
Sbjct: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFK 240
YPFYLMWRSPGKEGSHFNPYSDLFAP+ERKDIVISTSCWTLMAV+LVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
Query: 241 IYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI 300
+YGVPYWIFVMWLD VTYLHHHGYEQKLPWYRGEEW+YLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLV NLVNSL+QDHYVS
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS 360
Query: 361 DQGNIVFYQTDPYLYHY 378
DQGNIVFYQTDPYLYHY
Sbjct: 361 DQGNIVFYQTDPYLYHY 375
BLAST of Clc03G00730 vs. ExPASy TrEMBL
Match:
A0A0A0LVJ0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G532210 PE=3 SV=1)
HSP 1 Score: 745.0 bits (1922), Expect = 1.6e-211
Identity = 350/377 (92.84%), Postives = 363/377 (96.29%), Query Frame = 0
Query: 1 MAIPLPASGTGNGNGNATDAAPAPFDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPL A N NAT AAPAPFDPSAPPPFR++EIRAAIPPHCWVK+PWRSL+YVLR
Sbjct: 1 MAIPLLA------NANATAAAPAPFDPSAPPPFRISEIRAAIPPHCWVKSPWRSLLYVLR 60
Query: 61 DLAIVSALVAAAVFFDSWLFWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLI 120
DLAIVSALVAAA+FFDSWL WPIYW+AQGTMFWAIFVLGHDCGHGSFSNSSALN+FMGL+
Sbjct: 61 DLAIVSALVAAALFFDSWLVWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA 180
LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA
Sbjct: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFK 240
YPFYLMWRSPGKEGSHFNPYSDLFAP ER+DIVISTSCWTLMA +LVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPGERRDIVISTSCWTLMAALLVYLSFVFGPFQIFK 240
Query: 241 IYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI 300
+YGVPYWIFVMWLD VTYLHHHGYEQKLPWYRGEEW+YLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP HLVKNLVNSL+QDHYVS
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPTHLVKNLVNSLKQDHYVS 360
Query: 361 DQGNIVFYQTDPYLYHY 378
DQGNIVFYQTDPYLYHY
Sbjct: 361 DQGNIVFYQTDPYLYHY 371
BLAST of Clc03G00730 vs. ExPASy TrEMBL
Match:
A0A6J1I635 (omega-3 fatty acid desaturase, endoplasmic reticulum-like OS=Cucurbita maxima OX=3661 GN=LOC111469542 PE=3 SV=1)
HSP 1 Score: 732.6 bits (1890), Expect = 8.1e-208
Identity = 342/377 (90.72%), Postives = 359/377 (95.23%), Query Frame = 0
Query: 1 MAIPLPASGTGNGNGNATDAAPAPFDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPLPAS T NG AA APFDPSAPPPFR+AEIRAAIPPHCWVK+PWRSL+YVLR
Sbjct: 1 MAIPLPASATATANGT---AALAPFDPSAPPPFRIAEIRAAIPPHCWVKSPWRSLLYVLR 60
Query: 61 DLAIVSALVAAAVFFDSWLFWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLI 120
DLA+VSAL+AAAVFFDSWL WPIYWVAQGTMFWAIFVLGHDCGHGSFSNS+ALN +GL+
Sbjct: 61 DLAVVSALIAAAVFFDSWLLWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSTALNTLVGLV 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA 180
LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLE+RTRILRFTLPFPILA
Sbjct: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFK 240
YPFYLMWRSPGK+GSHFNP SDLFAPNERKDIVISTSCW LMA +LVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKKGSHFNPNSDLFAPNERKDIVISTSCWALMAAVLVYLSFVFGPFQIFK 240
Query: 241 IYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI 300
IYGVPYWIFVMWLD VTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 IYGVPYWIFVMWLDLVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHL++NLVNS R+DH+VS
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLMENLVNSFREDHFVS 360
Query: 361 DQGNIVFYQTDPYLYHY 378
D GNIVFYQTDPYL+H+
Sbjct: 361 DNGNIVFYQTDPYLHHH 374
BLAST of Clc03G00730 vs. ExPASy TrEMBL
Match:
A0A6J1HLT0 (omega-3 fatty acid desaturase, endoplasmic reticulum-like OS=Cucurbita moschata OX=3662 GN=LOC111464727 PE=3 SV=1)
HSP 1 Score: 727.6 bits (1877), Expect = 2.6e-206
Identity = 339/377 (89.92%), Postives = 358/377 (94.96%), Query Frame = 0
Query: 1 MAIPLPASGTGNGNGNATDAAPAPFDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPLPAS T NG AA APFDPSAPPPFR+AEIRAAIPPHCWVK+PWRSL+YVLR
Sbjct: 1 MAIPLPASATATANGT---AALAPFDPSAPPPFRIAEIRAAIPPHCWVKSPWRSLLYVLR 60
Query: 61 DLAIVSALVAAAVFFDSWLFWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLI 120
DLA+VSAL+AAAVFFDSWL WPIYWVAQGTMFWAIFVLGHDCGHGSFSNS+ALN +GL+
Sbjct: 61 DLAVVSALIAAAVFFDSWLLWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSTALNTLVGLV 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA 180
LHS ILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLE+RTRILRFTLPFPILA
Sbjct: 121 LHSSILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFK 240
YPFYLMWRSPGK+GSHFNP SDLFAPNERKDIVISTSCW LMA +LVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKKGSHFNPNSDLFAPNERKDIVISTSCWALMAAVLVYLSFVFGPFQIFK 240
Query: 241 IYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI 300
IYGVPYWIFVMWLD VTYLHHHG+EQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 IYGVPYWIFVMWLDLVTYLHHHGHEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGP+PIHL++NLVNS R+DH+VS
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPLPIHLIENLVNSFREDHFVS 360
Query: 361 DQGNIVFYQTDPYLYHY 378
D GNIVFYQTDPYL+H+
Sbjct: 361 DNGNIVFYQTDPYLHHH 374
BLAST of Clc03G00730 vs. TAIR 10
Match:
AT5G05580.1 (fatty acid desaturase 8 )
HSP 1 Score: 581.3 bits (1497), Expect = 5.8e-166
Identity = 256/350 (73.14%), Postives = 301/350 (86.00%), Query Frame = 0
Query: 25 FDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLRDLAIVSALVAAAVFFDSWLFWPIY 84
FDP APPPF +A+IRAAIP HCWVKNPW S+ YV+RD+AIV L A A +F++WL WP+Y
Sbjct: 81 FDPGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLY 140
Query: 85 WVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLILHSFILVPYHGWRISHRTHHQNHG 144
W AQGTMFWA+FVLGHDCGHGSFSN LN+ G +LHS ILVPYHGWRISHRTHHQNHG
Sbjct: 141 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 200
Query: 145 NVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSDLF 204
+VE DESW PL + YK LEK T++ RFTLPFP+LAYPFYL RSPGK+GSH++P SDLF
Sbjct: 201 HVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLF 260
Query: 205 APNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFKIYGVPYWIFVMWLDFVTYLHHHGY 264
P E+KD++ ST+CWT MA +LV L+FV GP Q+ K+YG+PYWIFVMWLDFVTYLHHHG+
Sbjct: 261 LPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHGH 320
Query: 265 EQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAA 324
E KLPWYRG+EWSYLRGGLTT+DRDYG NNIHHDIGTHVIHHLFPQIPHYHLVEAT+AA
Sbjct: 321 EDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLVEATEAA 380
Query: 325 KGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVSDQGNIVFYQTDPYL 375
K VLGKYYREPK SGP+P+HL+ +L+ S++QDH+VSD G++V+Y+ DP L
Sbjct: 381 KPVLGKYYREPKNSGPLPLHLLGSLIKSMKQDHFVSDTGDVVYYEADPKL 430
BLAST of Clc03G00730 vs. TAIR 10
Match:
AT2G29980.1 (fatty acid desaturase 3 )
HSP 1 Score: 576.2 bits (1484), Expect = 1.9e-164
Identity = 267/378 (70.63%), Postives = 315/378 (83.33%), Query Frame = 0
Query: 1 MAIPLPASGTGNGNGNATDAAPAP-FDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVL 60
M + + NG+ A D FDPSA PPF++ +IRAAIP HCWVK+P RS+ YV+
Sbjct: 1 MVVAMDQRTNVNGDPGAGDRKKEERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVV 60
Query: 61 RDLAIVSALVAAAVFFDSWLFWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGL 120
RD+ V+AL AAV+ DSW WP+YW AQGT+FWAIFVLGHDCGHGSFS+ LN+ +G
Sbjct: 61 RDIIAVAALAIAAVYVDSWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGH 120
Query: 121 ILHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPIL 180
ILHSFILVPYHGWRISHRTHHQNHG+VE DESWVPL ++ YK+L TR+LR+T+P P+L
Sbjct: 121 ILHSFILVPYHGWRISHRTHHQNHGHVENDESWVPLPERVYKKLPHSTRMLRYTVPLPML 180
Query: 181 AYPFYLMWRSPGKEGSHFNPYSDLFAPNERKDIVISTSCWTLMAVILVYLSFVFGPFQIF 240
AYP YL +RSPGKEGSHFNPYS LFAP+ERK I ST+CW++M V L+ LSFVFGP +
Sbjct: 181 AYPLYLCYRSPGKEGSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVL 240
Query: 241 KIYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHD 300
K+YGVPY IFVMWLD VTYLHHHG+++KLPWYRG+EWSYLRGGLTT+DRDYG+FNNIHHD
Sbjct: 241 KVYGVPYIIFVMWLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIFNNIHHD 300
Query: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYV 360
IGTHVIHHLFPQIPHYHLV+ATKAAK VLG+YYREPK SG IPIHLV++LV S+++DHYV
Sbjct: 301 IGTHVIHHLFPQIPHYHLVDATKAAKHVLGRYYREPKTSGAIPIHLVESLVASIKKDHYV 360
Query: 361 SDQGNIVFYQTDPYLYHY 378
SD G+IVFY+TDP LY Y
Sbjct: 361 SDTGDIVFYETDPDLYVY 378
BLAST of Clc03G00730 vs. TAIR 10
Match:
AT3G11170.1 (fatty acid desaturase 7 )
HSP 1 Score: 575.5 bits (1482), Expect = 3.2e-164
Identity = 254/351 (72.36%), Postives = 301/351 (85.75%), Query Frame = 0
Query: 25 FDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLRDLAIVSALVAAAVFFDSWLFWPIY 84
FDP APPPF +A+IRAAIP HCWVKNPW+SL YV+RD+AIV AL A A + ++W+ WP+Y
Sbjct: 88 FDPGAPPPFNLADIRAAIPKHCWVKNPWKSLSYVVRDVAIVFALAAGAAYLNNWIVWPLY 147
Query: 85 WVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLILHSFILVPYHGWRISHRTHHQNHG 144
W+AQGTMFWA+FVLGHDCGHGSFSN LN+ +G +LHS ILVPYHGWRISHRTHHQNHG
Sbjct: 148 WLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHG 207
Query: 145 NVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSDLF 204
+VE DESW P+++K Y L+K TR RFTLP +LAYPFYL RSPGK+GSH++P SDLF
Sbjct: 208 HVENDESWHPMSEKIYNTLDKPTRFFRFTLPLVMLAYPFYLWARSPGKKGSHYHPDSDLF 267
Query: 205 APNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFKIYGVPYWIFVMWLDFVTYLHHHGY 264
P ERKD++ ST+CWT MA +LV L+F GP Q+ K+YG+PYWI VMWLDFVTYLHHHG+
Sbjct: 268 LPKERKDVLTSTACWTAMAALLVCLNFTIGPIQMLKLYGIPYWINVMWLDFVTYLHHHGH 327
Query: 265 EQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAA 324
E KLPWYRG+EWSYLRGGLTT+DRDYGL NNIHHDIGTHVIHHLFPQIPHYHLVEAT+AA
Sbjct: 328 EDKLPWYRGKEWSYLRGGLTTLDRDYGLINNIHHDIGTHVIHHLFPQIPHYHLVEATEAA 387
Query: 325 KGVLGKYYREPKKSGPIPIHLVKNLVNSLRQDHYVSDQGNIVFYQTDPYLY 376
K VLGKYYREP KSGP+P+HL++ L S+++DHYVSD+G +V+Y+ DP LY
Sbjct: 388 KPVLGKYYREPDKSGPLPLHLLEILAKSIKEDHYVSDEGEVVYYKADPNLY 438
BLAST of Clc03G00730 vs. TAIR 10
Match:
AT5G05580.2 (fatty acid desaturase 8 )
HSP 1 Score: 501.9 bits (1291), Expect = 4.5e-142
Identity = 222/296 (75.00%), Postives = 254/296 (85.81%), Query Frame = 0
Query: 25 FDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVLRDLAIVSALVAAAVFFDSWLFWPIY 84
FDP APPPF +A+IRAAIP HCWVKNPW S+ YV+RD+AIV L A A +F++WL WP+Y
Sbjct: 81 FDPGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLY 140
Query: 85 WVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGLILHSFILVPYHGWRISHRTHHQNHG 144
W AQGTMFWA+FVLGHDCGHGSFSN LN+ G +LHS ILVPYHGWRISHRTHHQNHG
Sbjct: 141 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 200
Query: 145 NVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSDLF 204
+VE DESW PL + YK LEK T++ RFTLPFP+LAYPFYL RSPGK+GSH++P SDLF
Sbjct: 201 HVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLF 260
Query: 205 APNERKDIVISTSCWTLMAVILVYLSFVFGPFQIFKIYGVPYWIFVMWLDFVTYLHHHGY 264
P E+KD++ ST+CWT MA +LV L+FV GP Q+ K+YG+PYWIFVMWLDFVTYLHHHG+
Sbjct: 261 LPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHGH 320
Query: 265 EQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEA 321
E KLPWYRG+EWSYLRGGLTT+DRDYG NNIHHDIGTHVIHHLFPQIPHYHLVEA
Sbjct: 321 EDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLVEA 376
BLAST of Clc03G00730 vs. TAIR 10
Match:
AT2G29980.2 (fatty acid desaturase 3 )
HSP 1 Score: 393.3 bits (1009), Expect = 2.2e-109
Identity = 182/275 (66.18%), Postives = 219/275 (79.64%), Query Frame = 0
Query: 1 MAIPLPASGTGNGNGNATDAAPAP-FDPSAPPPFRMAEIRAAIPPHCWVKNPWRSLVYVL 60
M + + NG+ A D FDPSA PPF++ +IRAAIP HCWVK+P RS+ YV+
Sbjct: 1 MVVAMDQRTNVNGDPGAGDRKKEERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVV 60
Query: 61 RDLAIVSALVAAAVFFDSWLFWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSSALNNFMGL 120
RD+ V+AL AAV+ DSW WP+YW AQGT+FWAIFVLGHDCGHGSFS+ LN+ +G
Sbjct: 61 RDIIAVAALAIAAVYVDSWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGH 120
Query: 121 ILHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPIL 180
ILHSFILVPYHGWRISHRTHHQNHG+VE DESWVPL ++ YK+L TR+LR+T+P P+L
Sbjct: 121 ILHSFILVPYHGWRISHRTHHQNHGHVENDESWVPLPERVYKKLPHSTRMLRYTVPLPML 180
Query: 181 AYPFYLMWRSPGKEGSHFNPYSDLFAPNERKDIVISTSCWTLMAVILVYLSFVFGPFQIF 240
AYP YL +RSPGKEGSHFNPYS LFAP+ERK I ST+CW++M V L+ LSFVFGP +
Sbjct: 181 AYPLYLCYRSPGKEGSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVL 240
Query: 241 KIYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGE 275
K+YGVPY IFVMWLD VTYLHHHG+++KLPWYRG+
Sbjct: 241 KVYGVPYIIFVMWLDAVTYLHHHGHDEKLPWYRGK 275
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAA0031835.1 | 5.0e-220 | 95.49 | acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum [Cucumis me... | [more] |
XP_008457364.1 | 3.0e-217 | 94.96 | PREDICTED: acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum ... | [more] |
XP_038893495.1 | 3.3e-211 | 94.16 | LOW QUALITY PROTEIN: omega-3 fatty acid desaturase, endoplasmic reticulum-like [... | [more] |
XP_004145254.1 | 3.3e-211 | 92.84 | acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum [Cucumis sa... | [more] |
XP_022970609.1 | 1.7e-207 | 90.72 | omega-3 fatty acid desaturase, endoplasmic reticulum-like [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
P48626 | 6.7e-167 | 76.07 | Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Nicotiana tabacum OX=409... | [more] |
P48625 | 3.7e-165 | 72.18 | Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Glycine max OX=3847 GN=F... | [more] |
P32291 | 6.2e-165 | 73.28 | Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Vigna radiata var. radia... | [more] |
P48622 | 8.2e-165 | 73.14 | Temperature-sensitive sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloropla... | [more] |
P48624 | 1.2e-163 | 72.40 | Acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum OS=Brassica... | [more] |
Match Name | E-value | Identity | Description | |
A0A5A7SRR2 | 2.4e-220 | 95.49 | Acyl-lipid omega-3 desaturase (Cytochrome b5), endoplasmic reticulum OS=Cucumis ... | [more] |
A0A1S3C608 | 1.5e-217 | 94.96 | acyl-lipid omega-3 desaturase (Cytochrome b5), endoplasmic reticulum OS=Cucumis ... | [more] |
A0A0A0LVJ0 | 1.6e-211 | 92.84 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G532210 PE=3 SV=1 | [more] |
A0A6J1I635 | 8.1e-208 | 90.72 | omega-3 fatty acid desaturase, endoplasmic reticulum-like OS=Cucurbita maxima OX... | [more] |
A0A6J1HLT0 | 2.6e-206 | 89.92 | omega-3 fatty acid desaturase, endoplasmic reticulum-like OS=Cucurbita moschata ... | [more] |