Clc01G09240 (gene) Watermelon (cordophanus) v2

Overview
NameClc01G09240
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Description(1->3)-beta-glucan endohydrolase
LocationClcChr01: 10188170 .. 10190956 (-)
RNA-Seq ExpressionClc01G09240
SyntenyClc01G09240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTATCAAATGGCTGTTTCATTTCTCCTTTTGCTTTTCTCTCTTTTGTTATCTCTCCAGTTGCAGGCCACCGCTGAAGGTGAGAGCTGAATCTCAGCCGTTTTTCTTTTATTTTTGGTCTCCCCCAACTCTTTTGAGTTCTTTCCACTGAATCTGAGCTTTTTTTTTTTTTTTTTTTTCTGGAGGGCTTTGCCCTTTTTCTTGTTATCTTGGTGTCTGATTTCTCTCATGAGCTGTTTTCGTTTTATGAAATGTGATGTTTCCATGTTTTTATTGGAGTTCATTTTTCATGTCCATGTGAAACATGAGGAACCCAGATGAAGAAAAACAAAATCCCAGAAAAAAGGAAAAGTGAAAACTCCTCATTGAATGAGTTTGAATTTCTGTATGTCAGATGTTTGATTAAATGCTTCAAGAGAGGTGGTTGGATGGGTAGTGAATGATTGCTGTTTTGCTCTGTCATTTATGATGGACTTCGGAGTTTTAGTTGTTTGTAATGATGCAGTGTTGAACCTAATTTAGTATTAAGCCCAAATAGATTAAACTAATAGGTGAGGGTAAACAATTTTTTTTTTTTATATTTATTCTTAGCTCTCTATCTCTCTTACCTTGTAGCTGACCTATTCTCTCGGATGTTATATATTAAATTCAACACTAAATCTAAGAGTTTAGGCATATATTCCTTTTTATTTTTTTATTTTTTTATTTTGCAGATGTATTTGTTGGTGTGAATATAGCAACTGATCTTTCGGTTATGCCCCATCCAACACAAGTAGTAGCCCTCCTGAAAGCCCAACAAATTCGGCATGTTCGCTTATACGACGCGGATGGTGGGATGCTGATGGCTCTTGCAAACACAGGCATTCAAGTTATGGTAACTGTCCCTAATGAACAACTTCTAGGGATTGGACAATCAAACTCCACTGCAGCTAACTGGGTTAATCACAATGTCATAGCACATTACCCAGCTACTAATATCACATCCATTTCTGTTGGTTCTGAGGTTTTAACAACACTTCCTAATGCAGCTAAAATCCTTGTCAATGCCCTGAAATACATTCATTCTGCACTTGTAGCTTCCAACCTTGATCACCAGATCAAGGTCTCTACACCTCTTTCTTCTTCGATTATTCTCGATTCATTTCCCCCTTCGCAAGCCTTCTTCAATGCATCACTGGATTCTGTAATAGTCCCCCTGCTTGGTTTCTTGCAATCTACTAACTCCTTCCTTATGCTCAATGTGTATCCCTACTATGACTATATGCAATCTGACGGCTTTATTTTGTTGGATTATGCACTCTTAAAGCCTCTCCCTTCAAACAAAGAAGCTGTTGATTCCAACACGCTTCTTCGTTACACCAATGTTTTCGATGCGATGGTTGATGCAGCTTACTTTGCTATGGCCTCTCTAAATTTCACTAACATACCCATTATAGTATCAGAAACAGGCTGGCCATCAAAAGGTGGTTCTAACGAGCCAGATGCAACCCTGGAAAATGCGAACACTTACAATAGCAATTTAATCAGGCACGTGCTGAACAAAACTGGAACTCCTAAACATCCAGGGATTGCTGTTAGTACTTACATTTATGAGCTCTATAACGAGGACTTGAAACCTGGGCCTATCTCTGAGAAGAACTGGGGATTGTTTGATGCAAATGGAAAGCCTATATACATTCTGCACTTGACAGGATCGGGACTTGTGTTAGCGAATGATACGGCGAACCCGACGTACTGTGCTGCAAAGGAAGGTGCCGACCCAAAGATGCTGCAGGCTGCCTTAGATTGGGCATGTGGACCTGGCAGGGTGGATTGTTCAGCTTTGCTTCAAGGGCAGCCGTGCTATGAACCCGACAATGTGATGGCTCACGCAACATATGCTTTCAATACTTATTATCATCAATGGGGAAAGCGTCAGGTTCATGTGACTTCAATGGGGTGGCTGCAGTTACAACCACTAACCCAAGTAAGAGAAGACCCAGCAGAATTCATCACTTAGCTTGTTTTTTTCTCCTGTTGTTCTGCATATCTTTAGAGCTAACTTGTCGTTCTGTTGTTGTGATGAATACAGGTCATGGATCCTGCTCATTTTCAGAAAGGTAAACTTCATACATATTCTTATCAACTGATAGAATTGAAGCTTAAGTATCTAAGTTTGGTTGTGATTTGAGGATCTGAGCGAGGGTATGTTTGAAAGGCTTTTGTCATGGTCAAGATTACTTCCAACTTAAAACGCTTACAATGCTAGATTACTTTTAACCTAAACTAATGGTCAAGAAAGCCCATTTAGAAAACTATATGCATCAAAGTGATTAGTAATTAAGCACTTTTTACCAGAGTGATCAAAATACTTTAGCTAGAATTACTTATTTAAAATGCGCTTTCAAACCCATCTTAAGAACCATGTCAGAAACCTTCCCCACCTCTAATCCAAAAAAAAAAAAAAGAATTGTGTTAGATGATATAATATTAAATTTACATTCACCCATTGGTTTAAGTTTTTGTGTCAATTGGTGATTAAAGGTGTTACAGTTTTGTTATTGACATTGTGTCAACTGCCATTCATTGTGATATAAACTCGACCTGCAATTGGGAATCATTTATTGATGTTGAGAATTTCTGGGATTTACAGTGTTGGAAGGAATGGGACGATGGCAAACGTCACAGCCCCCATCAATGAACTCCACAAATGCAGATTCTTCTGCAACCGCTTTTGGGTATAATATTTTGAGTTGTGCAGTGATGATGGTTGGAGCCCTGCTTTGGAGTTCCATTTGGTTGTAG

mRNA sequence

ATGGACTATCAAATGGCTGTTTCATTTCTCCTTTTGCTTTTCTCTCTTTTGTTATCTCTCCAGTTGCAGGCCACCGCTGAAGATGTATTTGTTGGTGTGAATATAGCAACTGATCTTTCGGTTATGCCCCATCCAACACAAGTAGTAGCCCTCCTGAAAGCCCAACAAATTCGGCATGTTCGCTTATACGACGCGGATGGTGGGATGCTGATGGCTCTTGCAAACACAGGCATTCAAGTTATGGTAACTGTCCCTAATGAACAACTTCTAGGGATTGGACAATCAAACTCCACTGCAGCTAACTGGGTTAATCACAATGTCATAGCACATTACCCAGCTACTAATATCACATCCATTTCTGTTGGTTCTGAGGTTTTAACAACACTTCCTAATGCAGCTAAAATCCTTGTCAATGCCCTGAAATACATTCATTCTGCACTTGTAGCTTCCAACCTTGATCACCAGATCAAGGTCTCTACACCTCTTTCTTCTTCGATTATTCTCGATTCATTTCCCCCTTCGCAAGCCTTCTTCAATGCATCACTGGATTCTGTAATAGTCCCCCTGCTTGGTTTCTTGCAATCTACTAACTCCTTCCTTATGCTCAATGTGTATCCCTACTATGACTATATGCAATCTGACGGCTTTATTTTGTTGGATTATGCACTCTTAAAGCCTCTCCCTTCAAACAAAGAAGCTGTTGATTCCAACACGCTTCTTCGTTACACCAATGTTTTCGATGCGATGGTTGATGCAGCTTACTTTGCTATGGCCTCTCTAAATTTCACTAACATACCCATTATAGTATCAGAAACAGGCTGGCCATCAAAAGGTGGTTCTAACGAGCCAGATGCAACCCTGGAAAATGCGAACACTTACAATAGCAATTTAATCAGGCACGTGCTGAACAAAACTGGAACTCCTAAACATCCAGGGATTGCTGTTAGTACTTACATTTATGAGCTCTATAACGAGGACTTGAAACCTGGGCCTATCTCTGAGAAGAACTGGGGATTGTTTGATGCAAATGGAAAGCCTATATACATTCTGCACTTGACAGGATCGGGACTTGTGTTAGCGAATGATACGGCGAACCCGACGTACTGTGCTGCAAAGGAAGGTGCCGACCCAAAGATGCTGCAGGCTGCCTTAGATTGGGCATGTGGACCTGGCAGGGTGGATTGTTCAGCTTTGCTTCAAGGGCAGCCGTGCTATGAACCCGACAATGTGATGGCTCACGCAACATATGCTTTCAATACTTATTATCATCAATGGGGAAAGCGTCAGGTTCATGTGACTTCAATGGGGTGGCTGCAGTTACAACCACTAACCCAAGTCATGGATCCTGCTCATTTTCAGAAAGTGTTGGAAGGAATGGGACGATGGCAAACGTCACAGCCCCCATCAATGAACTCCACAAATGCAGATTCTTCTGCAACCGCTTTTGGGTATAATATTTTGAGTTGTGCAGTGATGATGGTTGGAGCCCTGCTTTGGAGTTCCATTTGGTTGTAG

Coding sequence (CDS)

ATGGACTATCAAATGGCTGTTTCATTTCTCCTTTTGCTTTTCTCTCTTTTGTTATCTCTCCAGTTGCAGGCCACCGCTGAAGATGTATTTGTTGGTGTGAATATAGCAACTGATCTTTCGGTTATGCCCCATCCAACACAAGTAGTAGCCCTCCTGAAAGCCCAACAAATTCGGCATGTTCGCTTATACGACGCGGATGGTGGGATGCTGATGGCTCTTGCAAACACAGGCATTCAAGTTATGGTAACTGTCCCTAATGAACAACTTCTAGGGATTGGACAATCAAACTCCACTGCAGCTAACTGGGTTAATCACAATGTCATAGCACATTACCCAGCTACTAATATCACATCCATTTCTGTTGGTTCTGAGGTTTTAACAACACTTCCTAATGCAGCTAAAATCCTTGTCAATGCCCTGAAATACATTCATTCTGCACTTGTAGCTTCCAACCTTGATCACCAGATCAAGGTCTCTACACCTCTTTCTTCTTCGATTATTCTCGATTCATTTCCCCCTTCGCAAGCCTTCTTCAATGCATCACTGGATTCTGTAATAGTCCCCCTGCTTGGTTTCTTGCAATCTACTAACTCCTTCCTTATGCTCAATGTGTATCCCTACTATGACTATATGCAATCTGACGGCTTTATTTTGTTGGATTATGCACTCTTAAAGCCTCTCCCTTCAAACAAAGAAGCTGTTGATTCCAACACGCTTCTTCGTTACACCAATGTTTTCGATGCGATGGTTGATGCAGCTTACTTTGCTATGGCCTCTCTAAATTTCACTAACATACCCATTATAGTATCAGAAACAGGCTGGCCATCAAAAGGTGGTTCTAACGAGCCAGATGCAACCCTGGAAAATGCGAACACTTACAATAGCAATTTAATCAGGCACGTGCTGAACAAAACTGGAACTCCTAAACATCCAGGGATTGCTGTTAGTACTTACATTTATGAGCTCTATAACGAGGACTTGAAACCTGGGCCTATCTCTGAGAAGAACTGGGGATTGTTTGATGCAAATGGAAAGCCTATATACATTCTGCACTTGACAGGATCGGGACTTGTGTTAGCGAATGATACGGCGAACCCGACGTACTGTGCTGCAAAGGAAGGTGCCGACCCAAAGATGCTGCAGGCTGCCTTAGATTGGGCATGTGGACCTGGCAGGGTGGATTGTTCAGCTTTGCTTCAAGGGCAGCCGTGCTATGAACCCGACAATGTGATGGCTCACGCAACATATGCTTTCAATACTTATTATCATCAATGGGGAAAGCGTCAGGTTCATGTGACTTCAATGGGGTGGCTGCAGTTACAACCACTAACCCAAGTCATGGATCCTGCTCATTTTCAGAAAGTGTTGGAAGGAATGGGACGATGGCAAACGTCACAGCCCCCATCAATGAACTCCACAAATGCAGATTCTTCTGCAACCGCTTTTGGGTATAATATTTTGAGTTGTGCAGTGATGATGGTTGGAGCCCTGCTTTGGAGTTCCATTTGGTTGTAG

Protein sequence

MDYQMAVSFLLLLFSLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHVRLYDADGGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSISVGSEVLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNASLDSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLLRYTNVFDAMVDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRHVLNKTGTPKHPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLANDTANPTYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNTYYHQWGKRQVHVTSMGWLQLQPLTQVMDPAHFQKVLEGMGRWQTSQPPSMNSTNADSSATAFGYNILSCAVMMVGALLWSSIWL
Homology
BLAST of Clc01G09240 vs. NCBI nr
Match: XP_022993237.1 (glucan endo-1,3-beta-glucosidase 2-like [Cucurbita maxima])

HSP 1 Score: 846.7 bits (2186), Expect = 1.1e-241
Identity = 433/498 (86.95%), Postives = 448/498 (89.96%), Query Frame = 0

Query: 7   VSFLLLLFSLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHVRLYDAD 66
           VSFLLL+FSL   L LQA AEDVFVGVNI TDLSVMPHPTQVVALLKAQQIRHVRLYDAD
Sbjct: 9   VSFLLLIFSL---LSLQAPAEDVFVGVNIGTDLSVMPHPTQVVALLKAQQIRHVRLYDAD 68

Query: 67  GGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSISVGSEVL 126
           GGMLMALANTGIQVMV VPNEQLLGIGQSNSTAANWVNHNVIAHYPATNIT+ISVGSEVL
Sbjct: 69  GGMLMALANTGIQVMVAVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITAISVGSEVL 128

Query: 127 TTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNASLDSVI 186
           T LPNAAKILVNA+KYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNASLDSVI
Sbjct: 129 TELPNAAKILVNAMKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNASLDSVI 188

Query: 187 VPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLLRYTNVF 246
           VPLLGFLQSTNSFLM NVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLLRYTNVF
Sbjct: 189 VPLLGFLQSTNSFLMFNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLLRYTNVF 248

Query: 247 DAMVDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRHVLNKTG 306
           DA+VDAAYFAMASLNFTNIPI+VSETGWPSKGG+NEPDATL+NANTYNSNLIRHVLNKTG
Sbjct: 249 DALVDAAYFAMASLNFTNIPIVVSETGWPSKGGANEPDATLDNANTYNSNLIRHVLNKTG 308

Query: 307 TPKHPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLANDTANP 366
           TPKHPG+AVSTYIYELYNED+KPGPISEKNWGLFDANGKPIYIL LTGSGLVLANDT NP
Sbjct: 309 TPKHPGVAVSTYIYELYNEDMKPGPISEKNWGLFDANGKPIYILRLTGSGLVLANDTMNP 368

Query: 367 TYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNTYYHQWG 426
           TYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFN YYHQ G
Sbjct: 369 TYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNAYYHQMG 428

Query: 427 KRQVHVTSMGWLQLQPLTQVMDPAHFQKVLEGMGRWQTSQPPSMNSTNADSSATAFGYNI 486
           K        G   +           F + +   G       PSMNST +DSSA+AFGYN+
Sbjct: 429 KASGSCDFNGVAAVTTSNPSHGSCSFSESVGRNGTMANITAPSMNSTKSDSSASAFGYNM 488

Query: 487 LSCAVMMVGALLWSSIWL 505
           LSCAVMM GALLW SIWL
Sbjct: 489 LSCAVMMFGALLWGSIWL 503

BLAST of Clc01G09240 vs. NCBI nr
Match: XP_038875822.1 (glucan endo-1,3-beta-glucosidase 2 [Benincasa hispida])

HSP 1 Score: 844.3 bits (2180), Expect = 5.3e-241
Identity = 440/508 (86.61%), Postives = 456/508 (89.76%), Query Frame = 0

Query: 1   MDYQMAVSFLLLLFSLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHV 60
           M+YQMAVSFLL +FS LLSLQL AT EDVFVGVNI T LSVMPHPTQV ALLKAQQIRHV
Sbjct: 10  MNYQMAVSFLLFIFS-LLSLQLHATPEDVFVGVNIGTALSVMPHPTQVAALLKAQQIRHV 69

Query: 61  RLYDADGGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSIS 120
           RLYDADGGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVN NV+AHYPATNIT+IS
Sbjct: 70  RLYDADGGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNRNVLAHYPATNITAIS 129

Query: 121 VGSEVLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA 180
           VG +VLTT+PNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA
Sbjct: 130 VGFDVLTTVPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA 189

Query: 181 SLDSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL 240
           SLDSVIVPLLGFLQSTNSFLMLNVYPYYDY+QS+GFILLDYALLKPLPSNKEAVDSNTLL
Sbjct: 190 SLDSVIVPLLGFLQSTNSFLMLNVYPYYDYLQSNGFILLDYALLKPLPSNKEAVDSNTLL 249

Query: 241 RYTNVFDAMVDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRH 300
           RYTNVFDAMVDAAYFAMASLNFTNIPI+VSETGWPSKGGSNEPDATLENANTYNSNLIRH
Sbjct: 250 RYTNVFDAMVDAAYFAMASLNFTNIPIVVSETGWPSKGGSNEPDATLENANTYNSNLIRH 309

Query: 301 VLNKTGTPKHPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLA 360
           VLNKTGTPKHPGIA STYIYELYNEDLKPGPISEKNWGLFDANGKPIYIL LTGSGLVLA
Sbjct: 310 VLNKTGTPKHPGIATSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILCLTGSGLVLA 369

Query: 361 NDTANPTYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT 420
           NDT N TYCAAKEGADP+MLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT
Sbjct: 370 NDTTNQTYCAAKEGADPRMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT 429

Query: 421 YYHQWGKRQVHVTSMGWLQLQPLTQVMDPAHFQKVLEGM----GRWQTSQPPSMNSTNAD 480
           YYHQ G+        G           +P+H   +  G     G       PSMNSTNAD
Sbjct: 430 YYHQMGRASGSCDFNG----VAAVTTTNPSHGSCLFSGSVGRNGTMANITAPSMNSTNAD 489

Query: 481 SSATAFGYNILSCAVMMVGALLWSSIWL 505
           SSATAFGY+ILS AVM+VGALL SSIWL
Sbjct: 490 SSATAFGYDILSYAVMVVGALLGSSIWL 512

BLAST of Clc01G09240 vs. NCBI nr
Match: XP_004140301.1 (glucan endo-1,3-beta-glucosidase 2 [Cucumis sativus] >KGN51058.1 hypothetical protein Csa_007802 [Cucumis sativus])

HSP 1 Score: 844.0 bits (2179), Expect = 6.9e-241
Identity = 435/504 (86.31%), Postives = 449/504 (89.09%), Query Frame = 0

Query: 1   MDYQMAVSFLLLLFSLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHV 60
           MDYQM  SFLL +FSLL  L LQAT EDVFVGVNI T+LSVMPHPTQV ALLKAQQIRHV
Sbjct: 1   MDYQMHFSFLLFIFSLLF-LLLQATPEDVFVGVNIGTELSVMPHPTQVAALLKAQQIRHV 60

Query: 61  RLYDADGGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSIS 120
           RLYDADGGMLMALANTGIQVMVT+PNEQ+LGIGQSNSTAANWVN NV+AHYPATNIT+IS
Sbjct: 61  RLYDADGGMLMALANTGIQVMVTIPNEQILGIGQSNSTAANWVNRNVLAHYPATNITAIS 120

Query: 121 VGSEVLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA 180
           VGS+VLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA
Sbjct: 121 VGSDVLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA 180

Query: 181 SLDSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL 240
           SLD VIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL
Sbjct: 181 SLDPVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL 240

Query: 241 RYTNVFDAMVDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRH 300
           RYTNVFDAM+DAAYFAMASLNFTNIPI+VSETGWPSKGGSNEPDATLENANTYNSNLIRH
Sbjct: 241 RYTNVFDAMIDAAYFAMASLNFTNIPIVVSETGWPSKGGSNEPDATLENANTYNSNLIRH 300

Query: 301 VLNKTGTPKHPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLA 360
           VLNKTGTPKHPGIA+STYIYELYNEDLKPGPISEKNWGLFDANGKP+YIL LTGSGLVLA
Sbjct: 301 VLNKTGTPKHPGIAISTYIYELYNEDLKPGPISEKNWGLFDANGKPVYILRLTGSGLVLA 360

Query: 361 NDTANPTYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT 420
           NDT N TYCAAKEG DP+MLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT
Sbjct: 361 NDTTNQTYCAAKEGVDPRMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT 420

Query: 421 YYHQWGKRQVHVTSMGWLQLQPLTQVMDPAHFQKVLEGMGRWQTSQPPSMNSTNADSSAT 480
           YY Q GK        G   +           F   +   G       PSMNSTN DSSAT
Sbjct: 421 YYLQMGKGSGSCDFNGVAAVTTTNPSHGSCSFSGSVGRNGTMANITAPSMNSTNTDSSAT 480

Query: 481 AFGYNILSCAVMMVGALLWSSIWL 505
           AF YNILSCAVMMVGALL SSIWL
Sbjct: 481 AFEYNILSCAVMMVGALLGSSIWL 503

BLAST of Clc01G09240 vs. NCBI nr
Match: XP_022141638.1 (glucan endo-1,3-beta-glucosidase 2-like [Momordica charantia])

HSP 1 Score: 838.2 bits (2164), Expect = 3.8e-239
Identity = 429/504 (85.12%), Postives = 454/504 (90.08%), Query Frame = 0

Query: 1   MDYQMAVSFLLLLFSLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHV 60
           M+YQM VSFLL++FSL   L LQATAEDVFVGVNI TDLSVMPHPTQVV LLKAQQIRHV
Sbjct: 1   MEYQMGVSFLLMVFSL---LSLQATAEDVFVGVNIGTDLSVMPHPTQVVTLLKAQQIRHV 60

Query: 61  RLYDADGGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSIS 120
           RLY+ADG MLMALANTGIQVMVTVPNEQ+LGIGQSN+TAANWVNHNVIAHYPATNIT+IS
Sbjct: 61  RLYEADGAMLMALANTGIQVMVTVPNEQILGIGQSNATAANWVNHNVIAHYPATNITAIS 120

Query: 121 VGSEVLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA 180
           VGSEVLTTLPNAAKILVNALKYIHSALVA+NLDHQIKVSTPLSSSIILDSFPPSQAFFN+
Sbjct: 121 VGSEVLTTLPNAAKILVNALKYIHSALVAANLDHQIKVSTPLSSSIILDSFPPSQAFFNS 180

Query: 181 SLDSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL 240
           SL SVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL
Sbjct: 181 SLSSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL 240

Query: 241 RYTNVFDAMVDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRH 300
           RY+NVFDAMVDAAY AMASLNFTNIPI+VSETGWPSKGGSNEPDATLENANTYNSNLIRH
Sbjct: 241 RYSNVFDAMVDAAYSAMASLNFTNIPIVVSETGWPSKGGSNEPDATLENANTYNSNLIRH 300

Query: 301 VLNKTGTPKHPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLA 360
           VLNKTGTPKHPGIAVSTYIYELYNED+KPGPISEKNWGLFDA+G+PIYIL LTGSGLVLA
Sbjct: 301 VLNKTGTPKHPGIAVSTYIYELYNEDMKPGPISEKNWGLFDASGRPIYILRLTGSGLVLA 360

Query: 361 NDTANPTYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT 420
           NDT NPTYC+AK+GADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHA+YAFNT
Sbjct: 361 NDTMNPTYCSAKDGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHASYAFNT 420

Query: 421 YYHQWGKRQVHVTSMGWLQLQPLTQVMDPAHFQKVLEGMGRWQTSQPPSMNSTNADSSAT 480
           YYHQ GK        G   +           F + +   G       PSMNSTN++SSA+
Sbjct: 421 YYHQMGKASGSCDFNGVAAVTTSNPSHGSCSFSESVGRNGTMANITAPSMNSTNSESSAS 480

Query: 481 AFGYNILSCAVMMVGALLWSSIWL 505
             G+NILSCAVMMVGALL +SIWL
Sbjct: 481 DLGHNILSCAVMMVGALLGTSIWL 501

BLAST of Clc01G09240 vs. NCBI nr
Match: KAA0062305.1 (glucan endo-1,3-beta-glucosidase 2 [Cucumis melo var. makuwa] >TYK26672.1 glucan endo-1,3-beta-glucosidase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 835.9 bits (2158), Expect = 1.9e-238
Identity = 431/504 (85.52%), Postives = 448/504 (88.89%), Query Frame = 0

Query: 1   MDYQMAVSFLLLLFSLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHV 60
           MDYQ  +SFLL +FSLL  L LQAT EDVFVGVNI T+LSVMPHPTQV ALLKAQQIRHV
Sbjct: 1   MDYQTPLSFLLFIFSLLF-LLLQATPEDVFVGVNIGTELSVMPHPTQVAALLKAQQIRHV 60

Query: 61  RLYDADGGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSIS 120
           RLYDADGGMLMALANTGIQVMVT+PNEQ+LGIGQSNSTAANWVN NV+AHYPATNIT+IS
Sbjct: 61  RLYDADGGMLMALANTGIQVMVTIPNEQILGIGQSNSTAANWVNRNVLAHYPATNITAIS 120

Query: 121 VGSEVLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA 180
           VG +VLTTLPNAAKILVNALKY+HSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA
Sbjct: 121 VGFDVLTTLPNAAKILVNALKYVHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA 180

Query: 181 SLDSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL 240
           SLDSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL
Sbjct: 181 SLDSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL 240

Query: 241 RYTNVFDAMVDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRH 300
           RYTNVFDAM+DAAYFAMASLNFTNIPI+VSETGWPSKGGSNEPDATLENANTYNSNLIRH
Sbjct: 241 RYTNVFDAMIDAAYFAMASLNFTNIPIVVSETGWPSKGGSNEPDATLENANTYNSNLIRH 300

Query: 301 VLNKTGTPKHPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLA 360
           VLNKTGTPKHPGIA+STYIYELYNEDLKPGPISE+NWGLFDANGKP+YIL LTGSGLVLA
Sbjct: 301 VLNKTGTPKHPGIAISTYIYELYNEDLKPGPISERNWGLFDANGKPVYILRLTGSGLVLA 360

Query: 361 NDTANPTYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT 420
           NDT N TYCAAKEG DP+MLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT
Sbjct: 361 NDTTNQTYCAAKEGVDPRMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT 420

Query: 421 YYHQWGKRQVHVTSMGWLQLQPLTQVMDPAHFQKVLEGMGRWQTSQPPSMNSTNADSSAT 480
           YY Q GK        G   +           F   +   G       PSMNSTN DSSAT
Sbjct: 421 YYLQMGKASGSCDFNGVAAVTTTNPSHGSCSFSGSVGRNGTMANITAPSMNSTNTDSSAT 480

Query: 481 AFGYNILSCAVMMVGALLWSSIWL 505
           AF YNILSCAVMMVGALL SSI L
Sbjct: 481 AFEYNILSCAVMMVGALLGSSIRL 503

BLAST of Clc01G09240 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 638.6 bits (1646), Expect = 5.8e-182
Identity = 335/507 (66.07%), Postives = 397/507 (78.30%), Query Frame = 0

Query: 12  LLFSLLLSLQLQATA---------EDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHVRL 71
           LL  LLLSL L   A         E  ++GVNI TDLS MPHPTQVVALLKAQ+IRH+RL
Sbjct: 4   LLHLLLLSLSLLVLASASPSPPADEGSYIGVNIGTDLSDMPHPTQVVALLKAQEIRHIRL 63

Query: 72  YDADGGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSISVG 131
           Y+AD G+L+ALANTGI+V++++PN+QLLGIGQSNSTAANWV  NVIAHYPAT IT++SVG
Sbjct: 64  YNADPGLLIALANTGIKVIISIPNDQLLGIGQSNSTAANWVKRNVIAHYPATMITAVSVG 123

Query: 132 SEVLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNASL 191
           SEVLT+L NAA +LV+A+K +H+AL+++NLD  IKVSTPLS+S+ILD FPPSQAFFN SL
Sbjct: 124 SEVLTSLSNAAPVLVSAIKNVHAALLSANLDKLIKVSTPLSTSLILDPFPPSQAFFNRSL 183

Query: 192 DSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLLRY 251
           ++VIVPLL FLQSTNS+LM+NVYPY DYMQS+G I LDYAL KP+P NKEAVD+NTL+RY
Sbjct: 184 NAVIVPLLSFLQSTNSYLMVNVYPYIDYMQSNGVIPLDYALFKPIPPNKEAVDANTLVRY 243

Query: 252 TNVFDAMVDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRHVL 311
           +N FDAMVDA YFAMA LNFTNIP++V+E+GWPSKG +NEPDATL+NANTYNSNLIRHVL
Sbjct: 244 SNAFDAMVDATYFAMAFLNFTNIPVLVTESGWPSKGETNEPDATLDNANTYNSNLIRHVL 303

Query: 312 NKTGTPKHPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLAND 371
           NKTGTPK PGIAVSTYIYELYNED K G +SEKNWGLF+ANG+P+Y+L LT SG VLAND
Sbjct: 304 NKTGTPKRPGIAVSTYIYELYNEDTKAG-LSEKNWGLFNANGEPVYVLRLTNSGSVLAND 363

Query: 372 TANPTYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNTYY 431
           T N TYC A+EGAD KMLQAALDWACGPG++DCS + QG+ CYEPDNV+AHA YAF+TYY
Sbjct: 364 TTNQTYCTAREGADTKMLQAALDWACGPGKIDCSPIKQGETCYEPDNVVAHANYAFDTYY 423

Query: 432 HQWGKRQVHVTSMGWLQLQPLTQVMDPAHFQKVL---EGMGRWQTS---QPPSMNSTNAD 491
           HQ G         G   +       DP+H   V     G GR  TS     PS NST + 
Sbjct: 424 HQTGNNPDACNFNGVASI----TTTDPSHGTCVFAGSRGNGRNGTSVNITAPSANSTTSS 483

Query: 492 SSATAFGY-----NILSCAVMMVGALL 499
              +   Y     +IL+  ++ V  +L
Sbjct: 484 GIRSDLYYSRGIWSILTVMILNVANIL 505

BLAST of Clc01G09240 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 608.2 bits (1567), Expect = 8.3e-173
Identity = 311/473 (65.75%), Postives = 372/473 (78.65%), Query Frame = 0

Query: 10  LLLLFSLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHVRLYDADGGM 69
           LLLLF  L +    A ++D  +GVNI T+++ MP PTQVVALLK+Q I  VRLYDAD  M
Sbjct: 4   LLLLFLFLFA--SSALSQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSM 63

Query: 70  LMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSISVGSEVLTTL 129
           L+A A+TG+QV+++VPN+QLLGI QSN+TAANWV  NV A+YPATNIT+I+VGSEVLT+L
Sbjct: 64  LLAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSL 123

Query: 130 PNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNASLDSVIVPL 189
            NAA +LV+ALKYI +ALV +NLD QIKVSTP SS+IILDSFPPSQAFFN + D VIVPL
Sbjct: 124 TNAASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDPVIVPL 183

Query: 190 LGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLLRYTNVFDAM 249
           L FLQST S L+LNVYPY+DY+QS+G I LDYAL +PL +NKEAVD+NTLL YTNVFDA+
Sbjct: 184 LKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAI 243

Query: 250 VDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRHVLNKTGTPK 309
           VDAAYFAM+ LNFTNIPI+V+E+GWPSKGG +E DAT+ENANTYNSNLI+HV+NKTGTPK
Sbjct: 244 VDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPK 303

Query: 310 HPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLANDTANPTYC 369
           HPG AV+TYIYELYNED +PGP+SEKNWGLF  NG P+Y L L G+G +LANDT N T+C
Sbjct: 304 HPGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQTFC 363

Query: 370 AAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNTYYHQWGKRQ 429
            AKE  D KMLQAALDWACGPG+VDCSAL+QG+ CYEPD+V+AH+TYAFN YY + GK  
Sbjct: 364 IAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQKMGKAS 423

Query: 430 VHVTSMGWLQLQPLTQVMDPAHFQKVLEG-------MGRWQTSQPPSMNSTNA 476
                 G           DP+    V  G       +G   ++  PS NST +
Sbjct: 424 GSCDFKG----VATVTTTDPSRGTCVFPGSAKSNQTLGNNTSALAPSANSTTS 470

BLAST of Clc01G09240 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 497.3 bits (1279), Expect = 2.1e-139
Identity = 256/492 (52.03%), Postives = 347/492 (70.53%), Query Frame = 0

Query: 9   FLLLLF-----SLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHVRLY 68
           F LLL      SL  ++++Q   +D FVG NI TD+S +  PT++V  L+AQ++ HVRLY
Sbjct: 17  FTLLLISANSSSLSHNIKVQEQDKDPFVGFNIGTDVSNLLSPTELVKFLQAQKVNHVRLY 76

Query: 69  DADGGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSISVGS 128
           DAD  +L ALA T ++V+++VPN QLL IG SNSTAA+W+  NV+A+YP T IT+ISVG 
Sbjct: 77  DADPELLKALAKTKVRVIISVPNNQLLAIGSSNSTAASWIGRNVVAYYPETLITAISVGD 136

Query: 129 EVLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNASLD 188
           EVLTT+P++A +L+ A++ +++ALVASNL  QIKVSTP ++SI+LD+FPPSQA+FN +  
Sbjct: 137 EVLTTVPSSAPLLLPAIESLYNALVASNLHTQIKVSTPHAASIMLDTFPPSQAYFNQTWH 196

Query: 189 SVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLLRYT 248
           S++VPLL FL  T S LM+N+YPYY YMQ+ G + LD  L +PL  +KE VD NTLL YT
Sbjct: 197 SIMVPLLQFLSKTGSPLMMNLYPYYVYMQNKGVVPLDNCLFEPLTPSKEMVDPNTLLHYT 256

Query: 249 NVFDAMVDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRHVLN 308
           NV DAMVDAAY +M +LN +++ ++V+E+GWPSKG S EP AT++NA+TYNSNLI+HV +
Sbjct: 257 NVLDAMVDAAYVSMKNLNVSDVAVLVTESGWPSKGDSKEPYATIDNADTYNSNLIKHVFD 316

Query: 309 KTGTPKHPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLANDT 368
           +TGTP HP +  S YIYEL+NEDL+  P+SE +WGLF  N  P+Y+LH++GSG  LANDT
Sbjct: 317 RTGTPLHPEMTSSVYIYELFNEDLRAPPVSEASWGLFYGNSTPVYLLHVSGSGTFLANDT 376

Query: 369 ANPTYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNTYYH 428
            N TYC A +G D K LQAALDWACGPGR +CS +  G+ CY+P+NV  HA++AFN+YY 
Sbjct: 377 TNQTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQ 436

Query: 429 QWGKRQVHVTSMGWLQLQPLTQVMDPAHFQKVLEGMGRWQTSQPPSMNSTN-ADSSATAF 488
           + G+        G      +    DP+H   +  G  +        +NST  A   AT+ 
Sbjct: 437 KEGRASGSCDFKG----VAMITTTDPSHGSCIFPGSKKVGNRTQTVVNSTEVAAGEATSR 496

Query: 489 GYNILSCAVMMV 495
             +   C  +M+
Sbjct: 497 SLSRGFCVTIMI 504

BLAST of Clc01G09240 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 1.0e-122
Identity = 223/410 (54.39%), Postives = 294/410 (71.71%), Query Frame = 0

Query: 15  SLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHVRLYDADGGMLMALA 74
           +LLL L + A +   F+GVNI TDL+ MP P+ +V LLK+QQI HVRLYDA+  ML A A
Sbjct: 10  ALLLLLSILACSNAAFIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAFA 69

Query: 75  NTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSISVGSEVLTTLPNAAK 134
           NT I+VMV V NE++L IG+  S AA WVN NV A+ P+TNIT+I+VGSEVLTT+P+ A 
Sbjct: 70  NTSIEVMVGVTNEEILKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVAP 129

Query: 135 ILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNASLDSVIVPLLGFLQ 194
           IL +AL  IH ALVASNL+ ++KVS+P+S  I+   FPPS + F+ S ++ +  LL FL+
Sbjct: 130 ILASALNNIHKALVASNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQLLQFLK 189

Query: 195 STNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLLRYTNVFDAMVDAAY 254
           +T SF MLN YPYY Y  ++G   LDYAL K L   K+ VD NTLL Y ++FDAMVDAAY
Sbjct: 190 NTGSFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAAY 249

Query: 255 FAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRHVLNKTGTPKHPGIA 314
           ++M +LNF+ IP++V+ETGWPS GGS+E  AT+ NA T+N+NLI+ VLN +G P  P I 
Sbjct: 250 YSMEALNFSKIPVVVTETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDIP 309

Query: 315 VSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLANDTANPTYCAAKEG 374
           ++TYIYELYNED + GP+SE+NWG+   NG  +Y L L+G G   A    +  +C AK  
Sbjct: 310 INTYIYELYNEDKRSGPVSERNWGILFPNGTSVYPLSLSG-GSSSAALNGSSMFCVAKAD 369

Query: 375 ADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNTYYHQ 425
           AD   L   L+WACG GR +C+A+  GQPCY P++V +HA++AFN YY +
Sbjct: 370 ADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQK 418

BLAST of Clc01G09240 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 1.1e-92
Identity = 197/515 (38.25%), Postives = 289/515 (56.12%), Query Frame = 0

Query: 31  VGVNIATDLSVMPHPTQVVALLKAQQIRHVRLYDADGGMLMALANTGIQVMVTVPNEQLL 90
           +G+    +   +P P +V  L++   I+ VR+YDA+  +L A ANTGI++M+ VPN  LL
Sbjct: 27  IGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPNADLL 86

Query: 91  GIGQSNSTAANWVNHNVIAHYPATNITSISVGSEVLTTLPNAAKILVNALKYIHSALVAS 150
              Q  S    W+++N++ +YP+T ITSISVG EV     NA  +++ A++ IH+AL  S
Sbjct: 87  AFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGLVLPAMRNIHTALKKS 146

Query: 151 NLDHQIKVSTPLSSSIILDSFPPSQAFFNASLDSVIVPLLGFLQSTNSFLMLNVYPYYDY 210
            LD +IK+S+  S +I+  SFPPS A F+    + + P+L FL    S  M+++YPYY Y
Sbjct: 147 GLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKPMLEFLVENESPFMIDLYPYYAY 206

Query: 211 MQSDGFILLDYALLKPLPSNKEAVDSNTLLRYTNVFDAMVDAAYFAMASLNFTNIPIIVS 270
             S   + L+YAL +   S+ + VD  T L Y+N+FDA +DA YFA+ +++F  + ++V+
Sbjct: 207 RDSTEKVPLEYALFE---SSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVT 266

Query: 271 ETGWPSKGGSNEPDATLENANTYNSNLIRHVLNKTGTPKHPGIAVSTYIYELYNEDLKPG 330
           E+GWPSKG   E  AT ENA  YN+NLIRHV+   GTP  PG  +  Y++ L+NE+ KPG
Sbjct: 267 ESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKPG 326

Query: 331 PISEKNWGLFDANGKPIYILHLTGSGLVLANDTANPT----------------------- 390
             SE+NWG+F ANG  +Y L  TG      + T + T                       
Sbjct: 327 IESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGGG 386

Query: 391 -------YCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT 450
                  +C A   A    LQ ALDWACGPG VDCSA+   QPC+EPD V++HA+YAFNT
Sbjct: 387 GGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNT 446

Query: 451 YYHQWGKRQVHVTSMGWLQLQPLTQVMDPAH------FQKVLEGMGRWQ----TSQPPSM 504
           YY Q G   +  +  G      +    DP++           +G  R      T    ++
Sbjct: 447 YYQQSGASSIDCSFNG----ASVEVDKDPSYGNCLYMIAPATDGFNRTMAGNITGNITAI 506

BLAST of Clc01G09240 vs. ExPASy TrEMBL
Match: A0A6J1JS82 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111489318 PE=3 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 5.1e-242
Identity = 433/498 (86.95%), Postives = 448/498 (89.96%), Query Frame = 0

Query: 7   VSFLLLLFSLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHVRLYDAD 66
           VSFLLL+FSL   L LQA AEDVFVGVNI TDLSVMPHPTQVVALLKAQQIRHVRLYDAD
Sbjct: 9   VSFLLLIFSL---LSLQAPAEDVFVGVNIGTDLSVMPHPTQVVALLKAQQIRHVRLYDAD 68

Query: 67  GGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSISVGSEVL 126
           GGMLMALANTGIQVMV VPNEQLLGIGQSNSTAANWVNHNVIAHYPATNIT+ISVGSEVL
Sbjct: 69  GGMLMALANTGIQVMVAVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITAISVGSEVL 128

Query: 127 TTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNASLDSVI 186
           T LPNAAKILVNA+KYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNASLDSVI
Sbjct: 129 TELPNAAKILVNAMKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNASLDSVI 188

Query: 187 VPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLLRYTNVF 246
           VPLLGFLQSTNSFLM NVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLLRYTNVF
Sbjct: 189 VPLLGFLQSTNSFLMFNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLLRYTNVF 248

Query: 247 DAMVDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRHVLNKTG 306
           DA+VDAAYFAMASLNFTNIPI+VSETGWPSKGG+NEPDATL+NANTYNSNLIRHVLNKTG
Sbjct: 249 DALVDAAYFAMASLNFTNIPIVVSETGWPSKGGANEPDATLDNANTYNSNLIRHVLNKTG 308

Query: 307 TPKHPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLANDTANP 366
           TPKHPG+AVSTYIYELYNED+KPGPISEKNWGLFDANGKPIYIL LTGSGLVLANDT NP
Sbjct: 309 TPKHPGVAVSTYIYELYNEDMKPGPISEKNWGLFDANGKPIYILRLTGSGLVLANDTMNP 368

Query: 367 TYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNTYYHQWG 426
           TYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFN YYHQ G
Sbjct: 369 TYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNAYYHQMG 428

Query: 427 KRQVHVTSMGWLQLQPLTQVMDPAHFQKVLEGMGRWQTSQPPSMNSTNADSSATAFGYNI 486
           K        G   +           F + +   G       PSMNST +DSSA+AFGYN+
Sbjct: 429 KASGSCDFNGVAAVTTSNPSHGSCSFSESVGRNGTMANITAPSMNSTKSDSSASAFGYNM 488

Query: 487 LSCAVMMVGALLWSSIWL 505
           LSCAVMM GALLW SIWL
Sbjct: 489 LSCAVMMFGALLWGSIWL 503

BLAST of Clc01G09240 vs. ExPASy TrEMBL
Match: A0A0A0KNB7 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G423880 PE=3 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 3.3e-241
Identity = 435/504 (86.31%), Postives = 449/504 (89.09%), Query Frame = 0

Query: 1   MDYQMAVSFLLLLFSLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHV 60
           MDYQM  SFLL +FSLL  L LQAT EDVFVGVNI T+LSVMPHPTQV ALLKAQQIRHV
Sbjct: 1   MDYQMHFSFLLFIFSLLF-LLLQATPEDVFVGVNIGTELSVMPHPTQVAALLKAQQIRHV 60

Query: 61  RLYDADGGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSIS 120
           RLYDADGGMLMALANTGIQVMVT+PNEQ+LGIGQSNSTAANWVN NV+AHYPATNIT+IS
Sbjct: 61  RLYDADGGMLMALANTGIQVMVTIPNEQILGIGQSNSTAANWVNRNVLAHYPATNITAIS 120

Query: 121 VGSEVLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA 180
           VGS+VLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA
Sbjct: 121 VGSDVLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA 180

Query: 181 SLDSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL 240
           SLD VIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL
Sbjct: 181 SLDPVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL 240

Query: 241 RYTNVFDAMVDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRH 300
           RYTNVFDAM+DAAYFAMASLNFTNIPI+VSETGWPSKGGSNEPDATLENANTYNSNLIRH
Sbjct: 241 RYTNVFDAMIDAAYFAMASLNFTNIPIVVSETGWPSKGGSNEPDATLENANTYNSNLIRH 300

Query: 301 VLNKTGTPKHPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLA 360
           VLNKTGTPKHPGIA+STYIYELYNEDLKPGPISEKNWGLFDANGKP+YIL LTGSGLVLA
Sbjct: 301 VLNKTGTPKHPGIAISTYIYELYNEDLKPGPISEKNWGLFDANGKPVYILRLTGSGLVLA 360

Query: 361 NDTANPTYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT 420
           NDT N TYCAAKEG DP+MLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT
Sbjct: 361 NDTTNQTYCAAKEGVDPRMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT 420

Query: 421 YYHQWGKRQVHVTSMGWLQLQPLTQVMDPAHFQKVLEGMGRWQTSQPPSMNSTNADSSAT 480
           YY Q GK        G   +           F   +   G       PSMNSTN DSSAT
Sbjct: 421 YYLQMGKGSGSCDFNGVAAVTTTNPSHGSCSFSGSVGRNGTMANITAPSMNSTNTDSSAT 480

Query: 481 AFGYNILSCAVMMVGALLWSSIWL 505
           AF YNILSCAVMMVGALL SSIWL
Sbjct: 481 AFEYNILSCAVMMVGALLGSSIWL 503

BLAST of Clc01G09240 vs. ExPASy TrEMBL
Match: A0A6J1CL34 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111011944 PE=3 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 1.8e-239
Identity = 429/504 (85.12%), Postives = 454/504 (90.08%), Query Frame = 0

Query: 1   MDYQMAVSFLLLLFSLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHV 60
           M+YQM VSFLL++FSL   L LQATAEDVFVGVNI TDLSVMPHPTQVV LLKAQQIRHV
Sbjct: 1   MEYQMGVSFLLMVFSL---LSLQATAEDVFVGVNIGTDLSVMPHPTQVVTLLKAQQIRHV 60

Query: 61  RLYDADGGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSIS 120
           RLY+ADG MLMALANTGIQVMVTVPNEQ+LGIGQSN+TAANWVNHNVIAHYPATNIT+IS
Sbjct: 61  RLYEADGAMLMALANTGIQVMVTVPNEQILGIGQSNATAANWVNHNVIAHYPATNITAIS 120

Query: 121 VGSEVLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA 180
           VGSEVLTTLPNAAKILVNALKYIHSALVA+NLDHQIKVSTPLSSSIILDSFPPSQAFFN+
Sbjct: 121 VGSEVLTTLPNAAKILVNALKYIHSALVAANLDHQIKVSTPLSSSIILDSFPPSQAFFNS 180

Query: 181 SLDSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL 240
           SL SVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL
Sbjct: 181 SLSSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL 240

Query: 241 RYTNVFDAMVDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRH 300
           RY+NVFDAMVDAAY AMASLNFTNIPI+VSETGWPSKGGSNEPDATLENANTYNSNLIRH
Sbjct: 241 RYSNVFDAMVDAAYSAMASLNFTNIPIVVSETGWPSKGGSNEPDATLENANTYNSNLIRH 300

Query: 301 VLNKTGTPKHPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLA 360
           VLNKTGTPKHPGIAVSTYIYELYNED+KPGPISEKNWGLFDA+G+PIYIL LTGSGLVLA
Sbjct: 301 VLNKTGTPKHPGIAVSTYIYELYNEDMKPGPISEKNWGLFDASGRPIYILRLTGSGLVLA 360

Query: 361 NDTANPTYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT 420
           NDT NPTYC+AK+GADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHA+YAFNT
Sbjct: 361 NDTMNPTYCSAKDGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHASYAFNT 420

Query: 421 YYHQWGKRQVHVTSMGWLQLQPLTQVMDPAHFQKVLEGMGRWQTSQPPSMNSTNADSSAT 480
           YYHQ GK        G   +           F + +   G       PSMNSTN++SSA+
Sbjct: 421 YYHQMGKASGSCDFNGVAAVTTSNPSHGSCSFSESVGRNGTMANITAPSMNSTNSESSAS 480

Query: 481 AFGYNILSCAVMMVGALLWSSIWL 505
             G+NILSCAVMMVGALL +SIWL
Sbjct: 481 DLGHNILSCAVMMVGALLGTSIWL 501

BLAST of Clc01G09240 vs. ExPASy TrEMBL
Match: A0A5D3DT62 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G003280 PE=3 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 9.1e-239
Identity = 431/504 (85.52%), Postives = 448/504 (88.89%), Query Frame = 0

Query: 1   MDYQMAVSFLLLLFSLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHV 60
           MDYQ  +SFLL +FSLL  L LQAT EDVFVGVNI T+LSVMPHPTQV ALLKAQQIRHV
Sbjct: 1   MDYQTPLSFLLFIFSLLF-LLLQATPEDVFVGVNIGTELSVMPHPTQVAALLKAQQIRHV 60

Query: 61  RLYDADGGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSIS 120
           RLYDADGGMLMALANTGIQVMVT+PNEQ+LGIGQSNSTAANWVN NV+AHYPATNIT+IS
Sbjct: 61  RLYDADGGMLMALANTGIQVMVTIPNEQILGIGQSNSTAANWVNRNVLAHYPATNITAIS 120

Query: 121 VGSEVLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA 180
           VG +VLTTLPNAAKILVNALKY+HSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA
Sbjct: 121 VGFDVLTTLPNAAKILVNALKYVHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNA 180

Query: 181 SLDSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL 240
           SLDSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL
Sbjct: 181 SLDSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLL 240

Query: 241 RYTNVFDAMVDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRH 300
           RYTNVFDAM+DAAYFAMASLNFTNIPI+VSETGWPSKGGSNEPDATLENANTYNSNLIRH
Sbjct: 241 RYTNVFDAMIDAAYFAMASLNFTNIPIVVSETGWPSKGGSNEPDATLENANTYNSNLIRH 300

Query: 301 VLNKTGTPKHPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLA 360
           VLNKTGTPKHPGIA+STYIYELYNEDLKPGPISE+NWGLFDANGKP+YIL LTGSGLVLA
Sbjct: 301 VLNKTGTPKHPGIAISTYIYELYNEDLKPGPISERNWGLFDANGKPVYILRLTGSGLVLA 360

Query: 361 NDTANPTYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT 420
           NDT N TYCAAKEG DP+MLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT
Sbjct: 361 NDTTNQTYCAAKEGVDPRMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNT 420

Query: 421 YYHQWGKRQVHVTSMGWLQLQPLTQVMDPAHFQKVLEGMGRWQTSQPPSMNSTNADSSAT 480
           YY Q GK        G   +           F   +   G       PSMNSTN DSSAT
Sbjct: 421 YYLQMGKASGSCDFNGVAAVTTTNPSHGSCSFSGSVGRNGTMANITAPSMNSTNTDSSAT 480

Query: 481 AFGYNILSCAVMMVGALLWSSIWL 505
           AF YNILSCAVMMVGALL SSI L
Sbjct: 481 AFEYNILSCAVMMVGALLGSSIRL 503

BLAST of Clc01G09240 vs. ExPASy TrEMBL
Match: A0A6J1FG01 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111445076 PE=3 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 3.8e-237
Identity = 431/507 (85.01%), Postives = 450/507 (88.76%), Query Frame = 0

Query: 1   MDYQM--AVSFLLLLFSLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIR 60
           M+ QM   VSFLLL+FSL   L LQA AEDVFVGVNI TDLSVMPHPTQVVALLKAQQIR
Sbjct: 1   MESQMGVCVSFLLLIFSL---LSLQAVAEDVFVGVNIGTDLSVMPHPTQVVALLKAQQIR 60

Query: 61  HVRLYDADGGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITS 120
           HVRLYDADGGMLMALANTGIQVMV+VPNEQLLGIGQSNSTAANWVNHNVIAHYPATNIT+
Sbjct: 61  HVRLYDADGGMLMALANTGIQVMVSVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITA 120

Query: 121 ISVGSEVLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFF 180
           ISVGSEVLT LPNAAKILVNA+KYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFF
Sbjct: 121 ISVGSEVLTELPNAAKILVNAMKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFF 180

Query: 181 NASLDSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNT 240
           NASLDSVIVPLLGFLQSTNSFLM NVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNT
Sbjct: 181 NASLDSVIVPLLGFLQSTNSFLMFNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNT 240

Query: 241 LLRYTNVFDAMVDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLI 300
           LLRYTNVFDA+VDAAYFAMASLNFTNIPI+VSETGWPSKGG+NEPDATL+NANTYNSNLI
Sbjct: 241 LLRYTNVFDALVDAAYFAMASLNFTNIPIVVSETGWPSKGGANEPDATLDNANTYNSNLI 300

Query: 301 RHVLNKTGTPKHPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLV 360
           RHVLNKTGTPKHPG+ VSTYIYELYNED+KPGPISEKNWGLFDANGKPIYIL LTGSGLV
Sbjct: 301 RHVLNKTGTPKHPGVPVSTYIYELYNEDMKPGPISEKNWGLFDANGKPIYILRLTGSGLV 360

Query: 361 LANDTANPTYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAF 420
           LANDT NPTYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAF
Sbjct: 361 LANDTMNPTYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAF 420

Query: 421 NTYYHQWGKRQVHVTSMGWLQLQPLTQVMDPAHFQKVLEGMGRWQTSQPPSMNSTNADSS 480
           N YYHQ GK        G   +           F + +   G       PSMNST +DSS
Sbjct: 421 NAYYHQMGKASGSCDFNGVAAVTTSNPSRGSCSFSESVGRNGTTANITAPSMNSTKSDSS 480

Query: 481 ATAFGYNILSCAVMMV-GALLWSSIWL 505
           A+ + YNILSCA+MM+ GALL  SIWL
Sbjct: 481 ASDYEYNILSCALMMMFGALLGGSIWL 504

BLAST of Clc01G09240 vs. TAIR 10
Match: AT1G66250.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 638.6 bits (1646), Expect = 4.1e-183
Identity = 335/507 (66.07%), Postives = 397/507 (78.30%), Query Frame = 0

Query: 12  LLFSLLLSLQLQATA---------EDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHVRL 71
           LL  LLLSL L   A         E  ++GVNI TDLS MPHPTQVVALLKAQ+IRH+RL
Sbjct: 4   LLHLLLLSLSLLVLASASPSPPADEGSYIGVNIGTDLSDMPHPTQVVALLKAQEIRHIRL 63

Query: 72  YDADGGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSISVG 131
           Y+AD G+L+ALANTGI+V++++PN+QLLGIGQSNSTAANWV  NVIAHYPAT IT++SVG
Sbjct: 64  YNADPGLLIALANTGIKVIISIPNDQLLGIGQSNSTAANWVKRNVIAHYPATMITAVSVG 123

Query: 132 SEVLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNASL 191
           SEVLT+L NAA +LV+A+K +H+AL+++NLD  IKVSTPLS+S+ILD FPPSQAFFN SL
Sbjct: 124 SEVLTSLSNAAPVLVSAIKNVHAALLSANLDKLIKVSTPLSTSLILDPFPPSQAFFNRSL 183

Query: 192 DSVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLLRY 251
           ++VIVPLL FLQSTNS+LM+NVYPY DYMQS+G I LDYAL KP+P NKEAVD+NTL+RY
Sbjct: 184 NAVIVPLLSFLQSTNSYLMVNVYPYIDYMQSNGVIPLDYALFKPIPPNKEAVDANTLVRY 243

Query: 252 TNVFDAMVDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRHVL 311
           +N FDAMVDA YFAMA LNFTNIP++V+E+GWPSKG +NEPDATL+NANTYNSNLIRHVL
Sbjct: 244 SNAFDAMVDATYFAMAFLNFTNIPVLVTESGWPSKGETNEPDATLDNANTYNSNLIRHVL 303

Query: 312 NKTGTPKHPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLAND 371
           NKTGTPK PGIAVSTYIYELYNED K G +SEKNWGLF+ANG+P+Y+L LT SG VLAND
Sbjct: 304 NKTGTPKRPGIAVSTYIYELYNEDTKAG-LSEKNWGLFNANGEPVYVLRLTNSGSVLAND 363

Query: 372 TANPTYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNTYY 431
           T N TYC A+EGAD KMLQAALDWACGPG++DCS + QG+ CYEPDNV+AHA YAF+TYY
Sbjct: 364 TTNQTYCTAREGADTKMLQAALDWACGPGKIDCSPIKQGETCYEPDNVVAHANYAFDTYY 423

Query: 432 HQWGKRQVHVTSMGWLQLQPLTQVMDPAHFQKVL---EGMGRWQTS---QPPSMNSTNAD 491
           HQ G         G   +       DP+H   V     G GR  TS     PS NST + 
Sbjct: 424 HQTGNNPDACNFNGVASI----TTTDPSHGTCVFAGSRGNGRNGTSVNITAPSANSTTSS 483

Query: 492 SSATAFGY-----NILSCAVMMVGALL 499
              +   Y     +IL+  ++ V  +L
Sbjct: 484 GIRSDLYYSRGIWSILTVMILNVANIL 505

BLAST of Clc01G09240 vs. TAIR 10
Match: AT2G01630.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 608.2 bits (1567), Expect = 5.9e-174
Identity = 311/473 (65.75%), Postives = 372/473 (78.65%), Query Frame = 0

Query: 10  LLLLFSLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHVRLYDADGGM 69
           LLLLF  L +    A ++D  +GVNI T+++ MP PTQVVALLK+Q I  VRLYDAD  M
Sbjct: 4   LLLLFLFLFA--SSALSQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSM 63

Query: 70  LMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSISVGSEVLTTL 129
           L+A A+TG+QV+++VPN+QLLGI QSN+TAANWV  NV A+YPATNIT+I+VGSEVLT+L
Sbjct: 64  LLAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSL 123

Query: 130 PNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNASLDSVIVPL 189
            NAA +LV+ALKYI +ALV +NLD QIKVSTP SS+IILDSFPPSQAFFN + D VIVPL
Sbjct: 124 TNAASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDPVIVPL 183

Query: 190 LGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLLRYTNVFDAM 249
           L FLQST S L+LNVYPY+DY+QS+G I LDYAL +PL +NKEAVD+NTLL YTNVFDA+
Sbjct: 184 LKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAI 243

Query: 250 VDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRHVLNKTGTPK 309
           VDAAYFAM+ LNFTNIPI+V+E+GWPSKGG +E DAT+ENANTYNSNLI+HV+NKTGTPK
Sbjct: 244 VDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPK 303

Query: 310 HPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLANDTANPTYC 369
           HPG AV+TYIYELYNED +PGP+SEKNWGLF  NG P+Y L L G+G +LANDT N T+C
Sbjct: 304 HPGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQTFC 363

Query: 370 AAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNTYYHQWGKRQ 429
            AKE  D KMLQAALDWACGPG+VDCSAL+QG+ CYEPD+V+AH+TYAFN YY + GK  
Sbjct: 364 IAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQKMGKAS 423

Query: 430 VHVTSMGWLQLQPLTQVMDPAHFQKVLEG-------MGRWQTSQPPSMNSTNA 476
                 G           DP+    V  G       +G   ++  PS NST +
Sbjct: 424 GSCDFKG----VATVTTTDPSRGTCVFPGSAKSNQTLGNNTSALAPSANSTTS 470

BLAST of Clc01G09240 vs. TAIR 10
Match: AT2G01630.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 554.3 bits (1427), Expect = 1.0e-157
Identity = 278/387 (71.83%), Postives = 328/387 (84.75%), Query Frame = 0

Query: 10  LLLLFSLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHVRLYDADGGM 69
           LLLLF  L +    A ++D  +GVNI T+++ MP PTQVVALLK+Q I  VRLYDAD  M
Sbjct: 4   LLLLFLFLFA--SSALSQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSM 63

Query: 70  LMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSISVGSEVLTTL 129
           L+A A+TG+QV+++VPN+QLLGI QSN+TAANWV  NV A+YPATNIT+I+VGSEVLT+L
Sbjct: 64  LLAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSL 123

Query: 130 PNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNASLDSVIVPL 189
            NAA +LV+ALKYI +ALV +NLD QIKVSTP SS+IILDSFPPSQAFFN + D VIVPL
Sbjct: 124 TNAASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDPVIVPL 183

Query: 190 LGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLLRYTNVFDAM 249
           L FLQST S L+LNVYPY+DY+QS+G I LDYAL +PL +NKEAVD+NTLL YTNVFDA+
Sbjct: 184 LKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAI 243

Query: 250 VDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRHVLNKTGTPK 309
           VDAAYFAM+ LNFTNIPI+V+E+GWPSKGG +E DAT+ENANTYNSNLI+HV+NKTGTPK
Sbjct: 244 VDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPK 303

Query: 310 HPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLANDTANPTYC 369
           HPG AV+TYIYELYNED +PGP+SEKNWGLF  NG P+Y L L G+G +LANDT N T+C
Sbjct: 304 HPGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQTFC 363

Query: 370 AAKEGADPKMLQAALDWACGPGRVDCS 397
            AKE  D KMLQAALDWACGPG+VD S
Sbjct: 364 IAKEKVDRKMLQAALDWACGPGKVDYS 388

BLAST of Clc01G09240 vs. TAIR 10
Match: AT1G11820.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 497.3 bits (1279), Expect = 1.5e-140
Identity = 256/492 (52.03%), Postives = 347/492 (70.53%), Query Frame = 0

Query: 9   FLLLLF-----SLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHVRLY 68
           F LLL      SL  ++++Q   +D FVG NI TD+S +  PT++V  L+AQ++ HVRLY
Sbjct: 17  FTLLLISANSSSLSHNIKVQEQDKDPFVGFNIGTDVSNLLSPTELVKFLQAQKVNHVRLY 76

Query: 69  DADGGMLMALANTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSISVGS 128
           DAD  +L ALA T ++V+++VPN QLL IG SNSTAA+W+  NV+A+YP T IT+ISVG 
Sbjct: 77  DADPELLKALAKTKVRVIISVPNNQLLAIGSSNSTAASWIGRNVVAYYPETLITAISVGD 136

Query: 129 EVLTTLPNAAKILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNASLD 188
           EVLTT+P++A +L+ A++ +++ALVASNL  QIKVSTP ++SI+LD+FPPSQA+FN +  
Sbjct: 137 EVLTTVPSSAPLLLPAIESLYNALVASNLHTQIKVSTPHAASIMLDTFPPSQAYFNQTWH 196

Query: 189 SVIVPLLGFLQSTNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLLRYT 248
           S++VPLL FL  T S LM+N+YPYY YMQ+ G + LD  L +PL  +KE VD NTLL YT
Sbjct: 197 SIMVPLLQFLSKTGSPLMMNLYPYYVYMQNKGVVPLDNCLFEPLTPSKEMVDPNTLLHYT 256

Query: 249 NVFDAMVDAAYFAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRHVLN 308
           NV DAMVDAAY +M +LN +++ ++V+E+GWPSKG S EP AT++NA+TYNSNLI+HV +
Sbjct: 257 NVLDAMVDAAYVSMKNLNVSDVAVLVTESGWPSKGDSKEPYATIDNADTYNSNLIKHVFD 316

Query: 309 KTGTPKHPGIAVSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLANDT 368
           +TGTP HP +  S YIYEL+NEDL+  P+SE +WGLF  N  P+Y+LH++GSG  LANDT
Sbjct: 317 RTGTPLHPEMTSSVYIYELFNEDLRAPPVSEASWGLFYGNSTPVYLLHVSGSGTFLANDT 376

Query: 369 ANPTYCAAKEGADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNTYYH 428
            N TYC A +G D K LQAALDWACGPGR +CS +  G+ CY+P+NV  HA++AFN+YY 
Sbjct: 377 TNQTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQ 436

Query: 429 QWGKRQVHVTSMGWLQLQPLTQVMDPAHFQKVLEGMGRWQTSQPPSMNSTN-ADSSATAF 488
           + G+        G      +    DP+H   +  G  +        +NST  A   AT+ 
Sbjct: 437 KEGRASGSCDFKG----VAMITTTDPSHGSCIFPGSKKVGNRTQTVVNSTEVAAGEATSR 496

Query: 489 GYNILSCAVMMV 495
             +   C  +M+
Sbjct: 497 SLSRGFCVTIMI 504

BLAST of Clc01G09240 vs. TAIR 10
Match: AT3G13560.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 441.8 bits (1135), Expect = 7.3e-124
Identity = 223/410 (54.39%), Postives = 294/410 (71.71%), Query Frame = 0

Query: 15  SLLLSLQLQATAEDVFVGVNIATDLSVMPHPTQVVALLKAQQIRHVRLYDADGGMLMALA 74
           +LLL L + A +   F+GVNI TDL+ MP P+ +V LLK+QQI HVRLYDA+  ML A A
Sbjct: 10  ALLLLLSILACSNAAFIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAFA 69

Query: 75  NTGIQVMVTVPNEQLLGIGQSNSTAANWVNHNVIAHYPATNITSISVGSEVLTTLPNAAK 134
           NT I+VMV V NE++L IG+  S AA WVN NV A+ P+TNIT+I+VGSEVLTT+P+ A 
Sbjct: 70  NTSIEVMVGVTNEEILKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVAP 129

Query: 135 ILVNALKYIHSALVASNLDHQIKVSTPLSSSIILDSFPPSQAFFNASLDSVIVPLLGFLQ 194
           IL +AL  IH ALVASNL+ ++KVS+P+S  I+   FPPS + F+ S ++ +  LL FL+
Sbjct: 130 ILASALNNIHKALVASNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQLLQFLK 189

Query: 195 STNSFLMLNVYPYYDYMQSDGFILLDYALLKPLPSNKEAVDSNTLLRYTNVFDAMVDAAY 254
           +T SF MLN YPYY Y  ++G   LDYAL K L   K+ VD NTLL Y ++FDAMVDAAY
Sbjct: 190 NTGSFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAAY 249

Query: 255 FAMASLNFTNIPIIVSETGWPSKGGSNEPDATLENANTYNSNLIRHVLNKTGTPKHPGIA 314
           ++M +LNF+ IP++V+ETGWPS GGS+E  AT+ NA T+N+NLI+ VLN +G P  P I 
Sbjct: 250 YSMEALNFSKIPVVVTETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDIP 309

Query: 315 VSTYIYELYNEDLKPGPISEKNWGLFDANGKPIYILHLTGSGLVLANDTANPTYCAAKEG 374
           ++TYIYELYNED + GP+SE+NWG+   NG  +Y L L+G G   A    +  +C AK  
Sbjct: 310 INTYIYELYNEDKRSGPVSERNWGILFPNGTSVYPLSLSG-GSSSAALNGSSMFCVAKAD 369

Query: 375 ADPKMLQAALDWACGPGRVDCSALLQGQPCYEPDNVMAHATYAFNTYYHQ 425
           AD   L   L+WACG GR +C+A+  GQPCY P++V +HA++AFN YY +
Sbjct: 370 ADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQK 418

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022993237.11.1e-24186.95glucan endo-1,3-beta-glucosidase 2-like [Cucurbita maxima][more]
XP_038875822.15.3e-24186.61glucan endo-1,3-beta-glucosidase 2 [Benincasa hispida][more]
XP_004140301.16.9e-24186.31glucan endo-1,3-beta-glucosidase 2 [Cucumis sativus] >KGN51058.1 hypothetical pr... [more]
XP_022141638.13.8e-23985.12glucan endo-1,3-beta-glucosidase 2-like [Momordica charantia][more]
KAA0062305.11.9e-23885.52glucan endo-1,3-beta-glucosidase 2 [Cucumis melo var. makuwa] >TYK26672.1 glucan... [more]
Match NameE-valueIdentityDescription
Q9C7U55.8e-18266.07Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ... [more]
Q9ZU918.3e-17365.75Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ... [more]
O653992.1e-13952.03Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ... [more]
Q94CD81.0e-12254.39Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ... [more]
Q8VYE51.1e-9238.25Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Match NameE-valueIdentityDescription
A0A6J1JS825.1e-24286.95(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111489318 PE=... [more]
A0A0A0KNB73.3e-24186.31(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G423880 PE=3... [more]
A0A6J1CL341.8e-23985.12(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111011944 ... [more]
A0A5D3DT629.1e-23985.52(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A6J1FG013.8e-23785.01(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111445076 P... [more]
Match NameE-valueIdentityDescription
AT1G66250.14.1e-18366.07O-Glycosyl hydrolases family 17 protein [more]
AT2G01630.15.9e-17465.75O-Glycosyl hydrolases family 17 protein [more]
AT2G01630.21.0e-15771.83O-Glycosyl hydrolases family 17 protein [more]
AT1G11820.21.5e-14052.03O-Glycosyl hydrolases family 17 protein [more]
AT3G13560.17.3e-12454.39O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 367..437
e-value: 3.5E-18
score: 76.4
IPR012946X8 domainPFAMPF07983X8coord: 368..428
e-value: 7.6E-13
score: 49.0
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 32..350
e-value: 1.8E-80
score: 270.9
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 265..278
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 30..355
e-value: 1.7E-114
score: 384.1
NoneNo IPR availablePANTHERPTHR32227:SF52GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 2coord: 19..489
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 19..489
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 32..350

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc01G09240.2Clc01G09240.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds