Cla97C04G074910 (gene) Watermelon (97103) v2.5

Overview
NameCla97C04G074910
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Descriptionvery-long-chain enoyl-CoA reductase-like
LocationCla97Chr04: 22534316 .. 22536258 (-)
RNA-Seq ExpressionCla97C04G074910
SyntenyCla97C04G074910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGTCTCCATTCTTTCCAGGAGTGGTAGAGAAATCATCACCGGCGGCCTTCAGATCGATGATTCGGTGAGCTTCTTGTTTTCGAGTTCTTCGCTTATCGTTTAACCTCGTTTTCGTTTGGTTCTACTTTAATCGCAGAGTTTATGTATGTTGTAGTGTAGTCGAAAAGTATCTTTATGCGATGGAGAATCGAAAATCGTAATCTCACTGTTTACTGGCTTCTTTTTTTTTTTATTTATTTATTTTTTTTTTTTTTTTTACTAATTTTCTACTGCTTTGCGATTTTGTAGGCTACGGTGAGCGACTTGCAGGATGCTATTCATCAGCGACGTAAGTTCTGGTTCCATTGTTCTGTTTCTCGATGACTAAGCTTTTATTTAGTGTTTTCTGCTATAATCATGGATTTTACTGAGATTTGAATCACCTGCTTACTCTTTCATATTGAAAATTCATCTAAATTGGAATGATTACAGAAATTTTAGTGCAGCAAGGTCTTAATATTTACTGTTACGCCTTCGATGAGTTAAAAACGGAGTTTAGATTTAACTTCCGATCATGTATTTTGCAATAGATTTAGCTTTCTATGGTATTTCTGGATTACTAAAGCATGAATTGATACAATACTCGCTGGCAATTCCTTCGGAATTTTGTTATAGCTTTCAGTAGTTTTTTTTTCCCATTTTCACTATGAATAAAGATTTATGTCTCTCAAACTCAGAAGCACTGATTGTACAATTCTCTTGTACTCTCATATCCAACAGGAAGAAAATACTATCCTTCTAGACAGCGTCTTACCCTGCCTGCCAAACCAGGGTCAAAAGAGAGGCCTATTGTTCTTATCCCAAGCAAGAAACTCGAAGATTACTGCGACGGAAATTTGGAGTACCTGACCATCACATTTAAGGATCTTGGTCCCCAGTTTTCTTATAGAATGCTCTTCTTGTTGGAGTATGTGGGGCCACTCCTTATCTATCCCATGTTTTACTACTTTCCCGTCTATAAGTTCTTCGGCTATAAAGACGAGCATCATATCTACCCTGCCCAGAGTTATGCCATGTACTATTGGTGTCTCCATTACTCTAAGCGGATCGTGGAAACCTTCTTTGTTCATAGGTTTAGTCATGGAACTTCACCCTTGTCCAATGTCTTCCGTAACTGTATGTACTATTGGAGTTTTGGAGCTCTGATAGGCTACTATGTTAACCACCCTCTTTACACCCCAGTGAATGACCTCCAAATGAAGATTGGCTTTGGATTTGGATTGTTATGTCAAATATCCAACTTATATTGCCATCTCATATTGAGGAATCTTAGAGATCCTAGTGGAAAGGGAGGCTACAAAATTCCAAATGGGTTCCTTTTCAGTATTGTAACTTGTGCCAACTACACTACTGAGATTTATCAATGGCTTGGATTCAATATCGCTACACAAACTGTTGCTGGTTATATTTTTGTAATGGCGGCTACCTACATAATGACTAAATGGGCAATTGCGAAACACCGCCGCTTGGAAAAGGTGATTACTATTTGTCTCGTTTGTTCATGTAAAATGAACTCCTTCCATTGATATTTTGAAAGGCTCTTACAGTAACTTTCATGCTTCTCGTAAGTTTTATGTTTAATTTAGTACACTTGGTCTTCAATTGTTGAAGTCTAGATCAAGCCGATTTTAATTTTGAAGAGAAAATCTCTGCGTAGGATTATCTGTGCGGTCTAATTGATCTTAACCAACAAATTGTTCCATCATGAACAATTGCTTTGGTTTCTTATCATGATCTGTGTTTTTTAAATTAAAATTTGTCTATAAAAAGTGATCTTGCTGCATCTACAGTTTTTGTTCTTCCCTTATATTCTAATGCTGTCTTCCGCAGCTTTTCGATGGGAAGGAAGGGAGGCCAAAGTATCCAAGAAGATGGGTGATTCTACCTCCATTTTTCTGA

mRNA sequence

ATGAAGGTCTCCATTCTTTCCAGGAGTGGTAGAGAAATCATCACCGGCGGCCTTCAGATCGATGATTCGTGTAGTCGAAAAGTATCTTTATGCGATGGAGAATCGAAAATCGCTACGGTGAGCGACTTGCAGGATGCTATTCATCAGCGACGAAGAAAATACTATCCTTCTAGACAGCGTCTTACCCTGCCTGCCAAACCAGGGTCAAAAGAGAGGCCTATTGTTCTTATCCCAAGCAAGAAACTCGAAGATTACTGCGACGGAAATTTGGAGTACCTGACCATCACATTTAAGGATCTTGGTCCCCAGTTTTCTTATAGAATGCTCTTCTTGTTGGAGTATGTGGGGCCACTCCTTATCTATCCCATGTTTTACTACTTTCCCGTCTATAAGTTCTTCGGCTATAAAGACGAGCATCATATCTACCCTGCCCAGAGTTATGCCATGTACTATTGGTGTCTCCATTACTCTAAGCGGATCGTGGAAACCTTCTTTGTTCATAGGTTTAGTCATGGAACTTCACCCTTGTCCAATGTCTTCCGTAACTGTATGTACTATTGGAGTTTTGGAGCTCTGATAGGCTACTATGTTAACCACCCTCTTTACACCCCAGTGAATGACCTCCAAATGAAGATTGGCTTTGGATTTGGATTGTTATGTCAAATATCCAACTTATATTGCCATCTCATATTGAGGAATCTTAGAGATCCTAGTGGAAAGGGAGGCTACAAAATTCCAAATGGGTTCCTTTTCAGTATTGTAACTTGTGCCAACTACACTACTGAGATTTATCAATGGCTTGGATTCAATATCGCTACACAAACTGTTGCTGGTTATATTTTTGTAATGGCGGCTACCTACATAATGACTAAATGGGCAATTGCGAAACACCGCCGCTTGGAAAAGCTTTTCGATGGGAAGGAAGGGAGGCCAAAGTATCCAAGAAGATGGGTGATTCTACCTCCATTTTTCTGA

Coding sequence (CDS)

ATGAAGGTCTCCATTCTTTCCAGGAGTGGTAGAGAAATCATCACCGGCGGCCTTCAGATCGATGATTCGTGTAGTCGAAAAGTATCTTTATGCGATGGAGAATCGAAAATCGCTACGGTGAGCGACTTGCAGGATGCTATTCATCAGCGACGAAGAAAATACTATCCTTCTAGACAGCGTCTTACCCTGCCTGCCAAACCAGGGTCAAAAGAGAGGCCTATTGTTCTTATCCCAAGCAAGAAACTCGAAGATTACTGCGACGGAAATTTGGAGTACCTGACCATCACATTTAAGGATCTTGGTCCCCAGTTTTCTTATAGAATGCTCTTCTTGTTGGAGTATGTGGGGCCACTCCTTATCTATCCCATGTTTTACTACTTTCCCGTCTATAAGTTCTTCGGCTATAAAGACGAGCATCATATCTACCCTGCCCAGAGTTATGCCATGTACTATTGGTGTCTCCATTACTCTAAGCGGATCGTGGAAACCTTCTTTGTTCATAGGTTTAGTCATGGAACTTCACCCTTGTCCAATGTCTTCCGTAACTGTATGTACTATTGGAGTTTTGGAGCTCTGATAGGCTACTATGTTAACCACCCTCTTTACACCCCAGTGAATGACCTCCAAATGAAGATTGGCTTTGGATTTGGATTGTTATGTCAAATATCCAACTTATATTGCCATCTCATATTGAGGAATCTTAGAGATCCTAGTGGAAAGGGAGGCTACAAAATTCCAAATGGGTTCCTTTTCAGTATTGTAACTTGTGCCAACTACACTACTGAGATTTATCAATGGCTTGGATTCAATATCGCTACACAAACTGTTGCTGGTTATATTTTTGTAATGGCGGCTACCTACATAATGACTAAATGGGCAATTGCGAAACACCGCCGCTTGGAAAAGCTTTTCGATGGGAAGGAAGGGAGGCCAAAGTATCCAAGAAGATGGGTGATTCTACCTCCATTTTTCTGA

Protein sequence

MKVSILSRSGREIITGGLQIDDSCSRKVSLCDGESKIATVSDLQDAIHQRRRKYYPSRQRLTLPAKPGSKERPIVLIPSKKLEDYCDGNLEYLTITFKDLGPQFSYRMLFLLEYVGPLLIYPMFYYFPVYKFFGYKDEHHIYPAQSYAMYYWCLHYSKRIVETFFVHRFSHGTSPLSNVFRNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGGYKIPNGFLFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEKLFDGKEGRPKYPRRWVILPPFF
Homology
BLAST of Cla97C04G074910 vs. NCBI nr
Match: XP_038877634.1 (very-long-chain enoyl-CoA reductase-like [Benincasa hispida])

HSP 1 Score: 600.5 bits (1547), Expect = 8.5e-168
Identity = 289/324 (89.20%), Postives = 302/324 (93.21%), Query Frame = 0

Query: 1   MKVSILSRSGREIITGGLQIDDSCSRKVSLCDGESKIATVSDLQDAIHQRRRKYYPSRQR 60
           MKV+ILSRSGREIITGGLQIDDS              ATVSDLQ+AIHQRRRKYYPSRQR
Sbjct: 1   MKVTILSRSGREIITGGLQIDDS--------------ATVSDLQEAIHQRRRKYYPSRQR 60

Query: 61  LTLPAKPGSKERPIVLIPSKKLEDYCDGNLEYLTITFKDLGPQFSYRMLFLLEYVGPLLI 120
           LTLPAKPGSKERPIVLIPSKKL+DYCDGNL+ L+ITFKDLG Q SYR LFLLEY+GPLLI
Sbjct: 61  LTLPAKPGSKERPIVLIPSKKLQDYCDGNLDNLSITFKDLGRQVSYRTLFLLEYLGPLLI 120

Query: 121 YPMFYYFPVYKFFGYKDEHHIYPAQSYAMYYWCLHYSKRIVETFFVHRFSHGTSPLSNVF 180
           YPMFYYFPVYKFFGY+DE HIYP Q+YAMYYWCLHYSKRI+ETFFVHRFSHGTSPLSNV 
Sbjct: 121 YPMFYYFPVYKFFGYQDERHIYPVQTYAMYYWCLHYSKRIMETFFVHRFSHGTSPLSNVV 180

Query: 181 RNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGK 240
           RNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLIL+NLRDPSG+
Sbjct: 181 RNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILKNLRDPSGE 240

Query: 241 GGYKIPNGFLFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRL 300
           GGYKIP GFLF+IVTCANYTTEIYQWLGFNIATQTVAGYIFV+AATYIMTKWAIAKHRRL
Sbjct: 241 GGYKIPRGFLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVIAATYIMTKWAIAKHRRL 300

Query: 301 EKLFDGKEGRPKYPRRWVILPPFF 325
           EKLFDGKEGRPKYPRRWVILPPFF
Sbjct: 301 EKLFDGKEGRPKYPRRWVILPPFF 310

BLAST of Cla97C04G074910 vs. NCBI nr
Match: XP_022143915.1 (very-long-chain enoyl-CoA reductase-like [Momordica charantia])

HSP 1 Score: 581.6 bits (1498), Expect = 4.1e-162
Identity = 277/324 (85.49%), Postives = 296/324 (91.36%), Query Frame = 0

Query: 1   MKVSILSRSGREIITGGLQIDDSCSRKVSLCDGESKIATVSDLQDAIHQRRRKYYPSRQR 60
           MKVSI+SRSGREII+GGLQIDDS              ATVSDLQ+AIHQRRRKYYP+RQR
Sbjct: 1   MKVSIVSRSGREIISGGLQIDDS--------------ATVSDLQEAIHQRRRKYYPARQR 60

Query: 61  LTLPAKPGSKERPIVLIPSKKLEDYCDGNLEYLTITFKDLGPQFSYRMLFLLEYVGPLLI 120
           LTLP KPGSKERP+VLIPSKKL+DYCDGNL+ LTITFKDLGPQ SYR LFLLEY+GPLLI
Sbjct: 61  LTLPTKPGSKERPVVLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLI 120

Query: 121 YPMFYYFPVYKFFGYKDEHHIYPAQSYAMYYWCLHYSKRIVETFFVHRFSHGTSPLSNVF 180
           YP  YYFPVYKFFGYK+E HIYP Q+YAM+YWCLHYSKRI+ETFFVHRFSH TSPLSNV 
Sbjct: 121 YPTIYYFPVYKFFGYKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVL 180

Query: 181 RNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGK 240
           RNC YYWSFGALI YYVNHPLYTPV+DLQMKIGFGFGL+CQISNLYCHLIL+NLRDPSGK
Sbjct: 181 RNCAYYWSFGALIAYYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGK 240

Query: 241 GGYKIPNGFLFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRL 300
           GGYKIP GFLF+IVTCANYTTEIYQWLGFNIAT+TVAGYIFVMAA YIM+KWAIAKHRRL
Sbjct: 241 GGYKIPRGFLFNIVTCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRL 300

Query: 301 EKLFDGKEGRPKYPRRWVILPPFF 325
           EKLFDGKEGRP+YPRRWVILPPFF
Sbjct: 301 EKLFDGKEGRPRYPRRWVILPPFF 310

BLAST of Cla97C04G074910 vs. NCBI nr
Match: KAE7996787.1 (hypothetical protein FH972_001478 [Carpinus fangiana])

HSP 1 Score: 516.2 bits (1328), Expect = 2.1e-142
Identity = 239/323 (73.99%), Postives = 273/323 (84.52%), Query Frame = 0

Query: 1   MKVSILSRSGREIITGGLQIDDSCSRKVSLCDGESKIATVSDLQDAIHQRRRKYYPSRQR 60
           MKVS++SRSG+E++ GGL   DS              ATV+DLQ+AIH+R RK+YPSRQR
Sbjct: 1   MKVSVVSRSGKELVKGGLDFSDS--------------ATVADLQEAIHRRTRKFYPSRQR 60

Query: 61  LTLPAKPGSKERPIVLIPSKKLEDYCDGNLEYLTITFKDLGPQFSYRMLFLLEYVGPLLI 120
           LTLP +PGSKE+PIVL   K LEDYC GNL++LT+ FKDLGPQ SYR LF  EY+GPL++
Sbjct: 61  LTLPLQPGSKEKPIVLNYKKSLEDYCHGNLDHLTVVFKDLGPQVSYRTLFFFEYLGPLIL 120

Query: 121 YPMFYYFPVYKFFGYKDEHHIYPAQSYAMYYWCLHYSKRIVETFFVHRFSHGTSPLSNVF 180
           YP+FYYFPVY++FGYK+E  I P Q+YA+YYWC HY KRI+ETFFVHRFSH TSPLSNVF
Sbjct: 121 YPIFYYFPVYQYFGYKEERVILPVQTYALYYWCFHYFKRIMETFFVHRFSHATSPLSNVF 180

Query: 181 RNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGK 240
           RNC YYWSFGA I YYVNHPLYTPV+DLQMKIGF FGLLCQ+SN YCHL+LRNLR P G 
Sbjct: 181 RNCAYYWSFGAYIAYYVNHPLYTPVSDLQMKIGFAFGLLCQVSNFYCHLMLRNLRSPDGN 240

Query: 241 GGYKIPNGFLFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRL 300
           GGY+IP GFLF+IVTCANYTTEIYQWLGFNIATQTVAGYIF++ A  IMT WA+AKHRRL
Sbjct: 241 GGYQIPRGFLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFLVVAAAIMTNWALAKHRRL 300

Query: 301 EKLFDGKEGRPKYPRRWVILPPF 324
           +KLFDGK+GRPKYPRRWVILPPF
Sbjct: 301 KKLFDGKDGRPKYPRRWVILPPF 309

BLAST of Cla97C04G074910 vs. NCBI nr
Match: TXG58604.1 (hypothetical protein EZV62_016433 [Acer yangbiense])

HSP 1 Score: 515.8 bits (1327), Expect = 2.8e-142
Identity = 240/323 (74.30%), Postives = 271/323 (83.90%), Query Frame = 0

Query: 1   MKVSILSRSGREIITGGLQIDDSCSRKVSLCDGESKIATVSDLQDAIHQRRRKYYPSRQR 60
           MKV+++SRSGREI+ GGL + DS              ATVSDLQ+ IH R +KYYPSRQR
Sbjct: 1   MKVTVVSRSGREIVKGGLDLHDS--------------ATVSDLQETIHGRCKKYYPSRQR 60

Query: 61  LTLPAKPGSKERPIVLIPSKKLEDYCDGNLEYLTITFKDLGPQFSYRMLFLLEYVGPLLI 120
           LTLP  PGSKERP VL P KK++DYCDGNL+ LT+TFKDLGPQ SYR LF  EY GPL+I
Sbjct: 61  LTLPLPPGSKERPTVLNPKKKIQDYCDGNLDNLTVTFKDLGPQVSYRTLFFWEYFGPLVI 120

Query: 121 YPMFYYFPVYKFFGYKDEHHIYPAQSYAMYYWCLHYSKRIVETFFVHRFSHGTSPLSNVF 180
           YP+FYYFPVYK FGY+ E  I+P Q+YAMYYWC HY KRI+ETFFVHRFSH TSPL+NVF
Sbjct: 121 YPIFYYFPVYKLFGYEGERAIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLANVF 180

Query: 181 RNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGK 240
           RNC YYWSFG++I YYVNHPLYTPVNDLQMKIGFGFG++CQISN YCHLIL+NLR   G 
Sbjct: 181 RNCAYYWSFGSVIAYYVNHPLYTPVNDLQMKIGFGFGIICQISNFYCHLILKNLRSTDGS 240

Query: 241 GGYKIPNGFLFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRL 300
            GY+IP GFLF+IVTCANYTTEIYQWLGFNIATQT+AGY F++ AT+IMT WA+AKHRRL
Sbjct: 241 SGYQIPRGFLFNIVTCANYTTEIYQWLGFNIATQTIAGYTFLVVATFIMTNWALAKHRRL 300

Query: 301 EKLFDGKEGRPKYPRRWVILPPF 324
           +KLFDGKEGR KYPRRWVILPPF
Sbjct: 301 KKLFDGKEGRTKYPRRWVILPPF 309

BLAST of Cla97C04G074910 vs. NCBI nr
Match: XP_042954556.1 (very-long-chain enoyl-CoA reductase-like [Carya illinoinensis] >KAG2730255.1 hypothetical protein I3760_01G283800 [Carya illinoinensis] >KAG6670087.1 hypothetical protein CIPAW_01G287000 [Carya illinoinensis] >KAG6734804.1 hypothetical protein I3842_01G288400 [Carya illinoinensis] >KAG7998886.1 hypothetical protein I3843_01G278000 [Carya illinoinensis])

HSP 1 Score: 513.8 bits (1322), Expect = 1.0e-141
Identity = 237/323 (73.37%), Postives = 275/323 (85.14%), Query Frame = 0

Query: 1   MKVSILSRSGREIITGGLQIDDSCSRKVSLCDGESKIATVSDLQDAIHQRRRKYYPSRQR 60
           MKV+++SRSGRE+I GGL++ DS              ATV+DLQ+AIHQR RK+YPSRQR
Sbjct: 1   MKVTVVSRSGREVIKGGLELSDS--------------ATVADLQEAIHQRTRKFYPSRQR 60

Query: 61  LTLPAKPGSKERPIVLIPSKKLEDYCDGNLEYLTITFKDLGPQFSYRMLFLLEYVGPLLI 120
           LTLP  PGSKERP+VL   K L+DYCDGNL++LT+ FKDLGPQ SY +LF  EYVGPL++
Sbjct: 61  LTLPLPPGSKERPVVLSYKKSLKDYCDGNLDHLTVVFKDLGPQVSYPILFFWEYVGPLVL 120

Query: 121 YPMFYYFPVYKFFGYKDEHHIYPAQSYAMYYWCLHYSKRIVETFFVHRFSHGTSPLSNVF 180
           YP+FYYFPVY++FGYK E  I+P Q+YA+YYWC HY KRI+ETFFVHRFSH TSPLSNVF
Sbjct: 121 YPIFYYFPVYRYFGYKGERVIHPVQTYALYYWCFHYFKRIMETFFVHRFSHATSPLSNVF 180

Query: 181 RNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGK 240
           RNC YYWSFG+ I Y VNHPLYTPV+DLQMKIGFGFGL CQISN YCH++LRNLR P+G 
Sbjct: 181 RNCAYYWSFGSYIAYCVNHPLYTPVSDLQMKIGFGFGLFCQISNFYCHMLLRNLRSPAGN 240

Query: 241 GGYKIPNGFLFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRL 300
           GGY+IP+GFLF+IVTCANYTTEIYQWLGFNIATQTVAGY F++ A  IMT WA+AKHRRL
Sbjct: 241 GGYQIPHGFLFNIVTCANYTTEIYQWLGFNIATQTVAGYSFLIVAALIMTNWALAKHRRL 300

Query: 301 EKLFDGKEGRPKYPRRWVILPPF 324
           +KLFDGK+GRP+YPRRWVILPPF
Sbjct: 301 KKLFDGKDGRPRYPRRWVILPPF 309

BLAST of Cla97C04G074910 vs. ExPASy Swiss-Prot
Match: Q9M2U2 (Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana OX=3702 GN=ECR PE=1 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 1.6e-137
Identity = 223/323 (69.04%), Postives = 264/323 (81.73%), Query Frame = 0

Query: 1   MKVSILSRSGREIITGGLQIDDSCSRKVSLCDGESKIATVSDLQDAIHQRRRKYYPSRQR 60
           MKV+++SRSGRE++   L + DS              ATV+DLQ+A H+R +K+YPSRQR
Sbjct: 1   MKVTVVSRSGREVLKAPLDLPDS--------------ATVADLQEAFHKRAKKFYPSRQR 60

Query: 61  LTLPAKPGSKERPIVLIPSKKLEDYCDGNLEYLTITFKDLGPQFSYRMLFLLEYVGPLLI 120
           LTLP  PGSK++P+VL   K L++YCDGN   LT+ FKDLG Q SYR LF  EY+GPLLI
Sbjct: 61  LTLPVTPGSKDKPVVLNSKKSLKEYCDGNNNSLTVVFKDLGAQVSYRTLFFFEYLGPLLI 120

Query: 121 YPMFYYFPVYKFFGYKDEHHIYPAQSYAMYYWCLHYSKRIVETFFVHRFSHGTSPLSNVF 180
           YP+FYYFPVYKF GY ++  I+P Q+YAMYYWC HY KRI+ETFFVHRFSH TSP+ NVF
Sbjct: 121 YPVFYYFPVYKFLGYGEDCVIHPVQTYAMYYWCFHYFKRILETFFVHRFSHATSPIGNVF 180

Query: 181 RNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGK 240
           RNC YYWSFGA I YYVNHPLYTPV+DLQMKIGFGFGL+CQ++N YCH++L+NLRDPSG 
Sbjct: 181 RNCAYYWSFGAYIAYYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHILLKNLRDPSGA 240

Query: 241 GGYKIPNGFLFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRL 300
           GGY+IP GFLF+IVTCANYTTEIYQWLGFNIATQT+AGY+F+  A  IMT WA+ KH RL
Sbjct: 241 GGYQIPRGFLFNIVTCANYTTEIYQWLGFNIATQTIAGYVFLAVAALIMTNWALGKHSRL 300

Query: 301 EKLFDGKEGRPKYPRRWVILPPF 324
            K+FDGK+G+PKYPRRWVILPPF
Sbjct: 301 RKIFDGKDGKPKYPRRWVILPPF 309

BLAST of Cla97C04G074910 vs. ExPASy Swiss-Prot
Match: Q55C17 (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn2 PE=3 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.2e-53
Identity = 121/290 (41.72%), Postives = 164/290 (56.55%), Query Frame = 0

Query: 35  SKIATVSDLQDAIHQRRRKYYPSRQRLTLPAKPGSKERPIVLIPSKKLEDYCDGNL-EYL 94
           S   TV +L+  I  + R     R RL +P+K  + + P       K  D    ++    
Sbjct: 21  SSSTTVGELKKQISSKTR-LGTERIRLAVPSK--TSKLPNAFEALGKDSDLVSKHVGADS 80

Query: 95  TITFKDLGPQFSYRMLFLLEYVGPLLIYPMFYYFPVYKFFGYKDEHHIYPAQSYAMYYWC 154
           T+ FKDLGPQ S+ ++F+ EY GPL +YP+FY+        Y  +     AQ  A+  + 
Sbjct: 81  TLYFKDLGPQISWSLVFICEYAGPLFVYPIFYFL---SNLIYGTDSPKSFAQKVALVCYS 140

Query: 155 LHYSKRIVETFFVHRFSHGTSPLSNVFRNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIG 214
           LHY KRI ET FVHRFSHGT P+ N+F+NC YYW   A++ Y+VNHPLYT     ++ +G
Sbjct: 141 LHYIKRIYETIFVHRFSHGTMPIFNLFKNCSYYWGCTAMVSYFVNHPLYTEAPIERVYLG 200

Query: 215 FGFGLLCQISNLYCHLILRNLRDPSGKGGYKIPNGFLFSIVTCANYTTEIYQWLGFNIAT 274
            G  ++ ++ N  CH+ LRNLR P+G    KIP G LF  V+C NYT EI  W+GF+I T
Sbjct: 201 LGLWIIGEVFNYICHIQLRNLR-PAGSTERKIPRGLLFEFVSCPNYTVEILSWIGFSILT 260

Query: 275 QTVAGYIFVMAATYIMTKWAIAKHRRLEKLFDGKEGRPKYPRRWVILPPF 324
           QT+  +IF +     M  WA+ KHRR  K F       KYP+   IL PF
Sbjct: 261 QTLTSWIFALMGAAQMWIWAVGKHRRYRKEFG-----DKYPKSRKILIPF 298

BLAST of Cla97C04G074910 vs. ExPASy Swiss-Prot
Match: Q9NZ01 (Very-long-chain enoyl-CoA reductase OS=Homo sapiens OX=9606 GN=TECR PE=1 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.3e-45
Identity = 120/314 (38.22%), Postives = 170/314 (54.14%), Query Frame = 0

Query: 20  IDDSCSRKVSLCDGESKIATVSDLQDAIHQRRRKYYPSRQRLTLPAKPGSKERPIVLIPS 79
           +D     K+   D     AT++++++   +   ++YP+RQ L L  K  S +   VL   
Sbjct: 9   LDAKTREKLCFLDKVEPHATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDVL--- 68

Query: 80  KKLEDYCDGNLEYLTITFKDLGPQFSYRMLFLLEYVGPLLIYPMFYYFPVYKFFGYKDEH 139
           +KL           T+ F+DLG Q S+  +FL EY GPL IY + +YF V   +G+K  +
Sbjct: 69  QKLPVGTTA-----TLYFRDLGAQISWVTVFLTEYAGPLFIY-LLFYFRVPFIYGHK--Y 128

Query: 140 HIYPAQSYAMYYWCL----HYSKRIVETFFVHRFSHGTSPLSNVFRNCMYYWSFGALIGY 199
               ++   ++  C+    HY KR++ET FVHRFSHGT PL N+F+NC YYW F A + Y
Sbjct: 129 DFTSSRHTVVHLACICHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAY 188

Query: 200 YVNHPLYTP--VNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGGYKIP----NGF 259
           Y+NHPLYTP      Q+K+     ++CQ+ N   H+ LR+LR P+G    KIP    N F
Sbjct: 189 YINHPLYTPPTYGAQQVKLALAIFVICQLGNFSIHMALRDLR-PAGSKTRKIPYPTKNPF 248

Query: 260 --LFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEKLFDGK 319
             LF +V+C NYT E+  W+GF I TQ +   +F +     MT WA  KHR   K F   
Sbjct: 249 TWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTIWAKGKHRSYLKEF--- 305

Query: 320 EGRPKYPRRWVILP 322
             R   P R  I+P
Sbjct: 309 --RDYPPLRMPIIP 305

BLAST of Cla97C04G074910 vs. ExPASy Swiss-Prot
Match: Q64232 (Very-long-chain enoyl-CoA reductase OS=Rattus norvegicus OX=10116 GN=Tecr PE=1 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 7.3e-45
Identity = 124/317 (39.12%), Postives = 173/317 (54.57%), Query Frame = 0

Query: 18  LQIDDSCSR-KVSLCDGESKIATVSDLQDAIHQRRRKYYPSRQRLTLPAKPGSKERPIVL 77
           ++I D+ +R K+   D     AT+S+++    +   ++YP+RQ L L  K  S +   VL
Sbjct: 6   VEIRDAKTREKLCFLDKVEPQATISEIKTLFTKTHPQWYPARQSLRLDPKGKSLKDEDVL 65

Query: 78  IPSKKLEDYCDGNLEYLTITFKDLGPQFSYRMLFLLEYVGPLLIYPMFYYFPVYKFFGYK 137
              +KL           T+ F+DLG Q S+  +FL EY GPL IY + +YF V   +G K
Sbjct: 66  ---QKLPVGTTA-----TLYFRDLGAQISWVTVFLTEYAGPLFIY-LLFYFRVPFIYGRK 125

Query: 138 DEHHIYPAQSYAMYYWCL----HYSKRIVETFFVHRFSHGTSPLSNVFRNCMYYWSFGAL 197
             +    ++   ++  C+    HY KR++ET FVHRFSHGT PL N+F+NC YYW F A 
Sbjct: 126 --YDFTSSRHTVVHLACMCHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAW 185

Query: 198 IGYYVNHPLYTP--VNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGGYKIP---- 257
           + YY+NHPLYTP      Q+K+     ++CQ+ N   H+ LR+LR P+G    KIP    
Sbjct: 186 MAYYINHPLYTPPTYGVQQVKLALAIFVICQLGNFSIHMALRDLR-PAGSKTRKIPYPTK 245

Query: 258 NGF--LFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEKLF 317
           N F  LF +V+C NYT E+  W+GF I TQ V   +F +     MT WA  KHR   K F
Sbjct: 246 NPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCVPVALFSLVGFTQMTIWAKGKHRSYLKEF 305

Query: 318 DGKEGRPKYPRRWVILP 322
                R   P R  I+P
Sbjct: 306 -----RDYPPLRMPIIP 305

BLAST of Cla97C04G074910 vs. ExPASy Swiss-Prot
Match: Q9CY27 (Very-long-chain enoyl-CoA reductase OS=Mus musculus OX=10090 GN=Tecr PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 9.5e-45
Identity = 124/317 (39.12%), Postives = 173/317 (54.57%), Query Frame = 0

Query: 18  LQIDDSCSR-KVSLCDGESKIATVSDLQDAIHQRRRKYYPSRQRLTLPAKPGSKERPIVL 77
           ++I D+ +R K+   D     AT+S+++    +   ++YP+RQ L L  K  S +   VL
Sbjct: 6   VEIRDAKTREKLCFLDKVEPQATISEIKTLFTKTHPQWYPARQSLRLDPKGKSLKDEDVL 65

Query: 78  IPSKKLEDYCDGNLEYLTITFKDLGPQFSYRMLFLLEYVGPLLIYPMFYYFPVYKFFGYK 137
              +KL           T+ F+DLG Q S+  +FL EY GPL IY + +YF V   +G K
Sbjct: 66  ---QKLPVGTTA-----TLYFRDLGAQISWVTVFLTEYAGPLFIY-LLFYFRVPFIYGRK 125

Query: 138 DEHHIYPAQSYAMYYWCL----HYSKRIVETFFVHRFSHGTSPLSNVFRNCMYYWSFGAL 197
             +    ++   ++  C+    HY KR++ET FVHRFSHGT PL N+F+NC YYW F A 
Sbjct: 126 --YDFTSSRHTVVHLACMCHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAW 185

Query: 198 IGYYVNHPLYTP--VNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGGYKIP---- 257
           + YY+NHPLYTP      Q+K+     ++CQ+ N   H+ LR+LR P+G    KIP    
Sbjct: 186 MAYYINHPLYTPPTYGVQQVKLALAVFVICQLGNFSIHMALRDLR-PAGSKTRKIPYPTK 245

Query: 258 NGF--LFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEKLF 317
           N F  LF +V+C NYT E+  W+GF I TQ V   +F +     MT WA  KHR   K F
Sbjct: 246 NPFTWLFLLVSCPNYTYEVGSWIGFAILTQCVPVALFSLVGFTQMTIWAKGKHRSYLKEF 305

Query: 318 DGKEGRPKYPRRWVILP 322
                R   P R  I+P
Sbjct: 306 -----RDYPPLRMPIIP 305

BLAST of Cla97C04G074910 vs. ExPASy TrEMBL
Match: A0A6J1CQ63 (very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC111013716 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.0e-162
Identity = 277/324 (85.49%), Postives = 296/324 (91.36%), Query Frame = 0

Query: 1   MKVSILSRSGREIITGGLQIDDSCSRKVSLCDGESKIATVSDLQDAIHQRRRKYYPSRQR 60
           MKVSI+SRSGREII+GGLQIDDS              ATVSDLQ+AIHQRRRKYYP+RQR
Sbjct: 1   MKVSIVSRSGREIISGGLQIDDS--------------ATVSDLQEAIHQRRRKYYPARQR 60

Query: 61  LTLPAKPGSKERPIVLIPSKKLEDYCDGNLEYLTITFKDLGPQFSYRMLFLLEYVGPLLI 120
           LTLP KPGSKERP+VLIPSKKL+DYCDGNL+ LTITFKDLGPQ SYR LFLLEY+GPLLI
Sbjct: 61  LTLPTKPGSKERPVVLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLI 120

Query: 121 YPMFYYFPVYKFFGYKDEHHIYPAQSYAMYYWCLHYSKRIVETFFVHRFSHGTSPLSNVF 180
           YP  YYFPVYKFFGYK+E HIYP Q+YAM+YWCLHYSKRI+ETFFVHRFSH TSPLSNV 
Sbjct: 121 YPTIYYFPVYKFFGYKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVL 180

Query: 181 RNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGK 240
           RNC YYWSFGALI YYVNHPLYTPV+DLQMKIGFGFGL+CQISNLYCHLIL+NLRDPSGK
Sbjct: 181 RNCAYYWSFGALIAYYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGK 240

Query: 241 GGYKIPNGFLFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRL 300
           GGYKIP GFLF+IVTCANYTTEIYQWLGFNIAT+TVAGYIFVMAA YIM+KWAIAKHRRL
Sbjct: 241 GGYKIPRGFLFNIVTCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRL 300

Query: 301 EKLFDGKEGRPKYPRRWVILPPFF 325
           EKLFDGKEGRP+YPRRWVILPPFF
Sbjct: 301 EKLFDGKEGRPRYPRRWVILPPFF 310

BLAST of Cla97C04G074910 vs. ExPASy TrEMBL
Match: A0A5N6QC86 (S5A_REDUCTASE domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_001478 PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 1.0e-142
Identity = 239/323 (73.99%), Postives = 273/323 (84.52%), Query Frame = 0

Query: 1   MKVSILSRSGREIITGGLQIDDSCSRKVSLCDGESKIATVSDLQDAIHQRRRKYYPSRQR 60
           MKVS++SRSG+E++ GGL   DS              ATV+DLQ+AIH+R RK+YPSRQR
Sbjct: 1   MKVSVVSRSGKELVKGGLDFSDS--------------ATVADLQEAIHRRTRKFYPSRQR 60

Query: 61  LTLPAKPGSKERPIVLIPSKKLEDYCDGNLEYLTITFKDLGPQFSYRMLFLLEYVGPLLI 120
           LTLP +PGSKE+PIVL   K LEDYC GNL++LT+ FKDLGPQ SYR LF  EY+GPL++
Sbjct: 61  LTLPLQPGSKEKPIVLNYKKSLEDYCHGNLDHLTVVFKDLGPQVSYRTLFFFEYLGPLIL 120

Query: 121 YPMFYYFPVYKFFGYKDEHHIYPAQSYAMYYWCLHYSKRIVETFFVHRFSHGTSPLSNVF 180
           YP+FYYFPVY++FGYK+E  I P Q+YA+YYWC HY KRI+ETFFVHRFSH TSPLSNVF
Sbjct: 121 YPIFYYFPVYQYFGYKEERVILPVQTYALYYWCFHYFKRIMETFFVHRFSHATSPLSNVF 180

Query: 181 RNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGK 240
           RNC YYWSFGA I YYVNHPLYTPV+DLQMKIGF FGLLCQ+SN YCHL+LRNLR P G 
Sbjct: 181 RNCAYYWSFGAYIAYYVNHPLYTPVSDLQMKIGFAFGLLCQVSNFYCHLMLRNLRSPDGN 240

Query: 241 GGYKIPNGFLFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRL 300
           GGY+IP GFLF+IVTCANYTTEIYQWLGFNIATQTVAGYIF++ A  IMT WA+AKHRRL
Sbjct: 241 GGYQIPRGFLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFLVVAAAIMTNWALAKHRRL 300

Query: 301 EKLFDGKEGRPKYPRRWVILPPF 324
           +KLFDGK+GRPKYPRRWVILPPF
Sbjct: 301 KKLFDGKDGRPKYPRRWVILPPF 309

BLAST of Cla97C04G074910 vs. ExPASy TrEMBL
Match: A0A5C7HP47 (S5A_REDUCTASE domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_016433 PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.3e-142
Identity = 240/323 (74.30%), Postives = 271/323 (83.90%), Query Frame = 0

Query: 1   MKVSILSRSGREIITGGLQIDDSCSRKVSLCDGESKIATVSDLQDAIHQRRRKYYPSRQR 60
           MKV+++SRSGREI+ GGL + DS              ATVSDLQ+ IH R +KYYPSRQR
Sbjct: 1   MKVTVVSRSGREIVKGGLDLHDS--------------ATVSDLQETIHGRCKKYYPSRQR 60

Query: 61  LTLPAKPGSKERPIVLIPSKKLEDYCDGNLEYLTITFKDLGPQFSYRMLFLLEYVGPLLI 120
           LTLP  PGSKERP VL P KK++DYCDGNL+ LT+TFKDLGPQ SYR LF  EY GPL+I
Sbjct: 61  LTLPLPPGSKERPTVLNPKKKIQDYCDGNLDNLTVTFKDLGPQVSYRTLFFWEYFGPLVI 120

Query: 121 YPMFYYFPVYKFFGYKDEHHIYPAQSYAMYYWCLHYSKRIVETFFVHRFSHGTSPLSNVF 180
           YP+FYYFPVYK FGY+ E  I+P Q+YAMYYWC HY KRI+ETFFVHRFSH TSPL+NVF
Sbjct: 121 YPIFYYFPVYKLFGYEGERAIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLANVF 180

Query: 181 RNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGK 240
           RNC YYWSFG++I YYVNHPLYTPVNDLQMKIGFGFG++CQISN YCHLIL+NLR   G 
Sbjct: 181 RNCAYYWSFGSVIAYYVNHPLYTPVNDLQMKIGFGFGIICQISNFYCHLILKNLRSTDGS 240

Query: 241 GGYKIPNGFLFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRL 300
            GY+IP GFLF+IVTCANYTTEIYQWLGFNIATQT+AGY F++ AT+IMT WA+AKHRRL
Sbjct: 241 SGYQIPRGFLFNIVTCANYTTEIYQWLGFNIATQTIAGYTFLVVATFIMTNWALAKHRRL 300

Query: 301 EKLFDGKEGRPKYPRRWVILPPF 324
           +KLFDGKEGR KYPRRWVILPPF
Sbjct: 301 KKLFDGKEGRTKYPRRWVILPPF 309

BLAST of Cla97C04G074910 vs. ExPASy TrEMBL
Match: M0T4A4 (S5A_REDUCTASE domain-containing protein OS=Musa acuminata subsp. malaccensis OX=214687 GN=103987000 PE=3 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 1.5e-141
Identity = 234/323 (72.45%), Postives = 275/323 (85.14%), Query Frame = 0

Query: 1   MKVSILSRSGREIITGGLQIDDSCSRKVSLCDGESKIATVSDLQDAIHQRRRKYYPSRQR 60
           MKV+++SRSGRE+I GG+++ D               ATVSDLQ+AIH R +KYYPSRQR
Sbjct: 1   MKVTVVSRSGREVIKGGIELHDE--------------ATVSDLQEAIHARTKKYYPSRQR 60

Query: 61  LTLPAKPGSKERPIVLIPSKKLEDYCDGNLEYLTITFKDLGPQFSYRMLFLLEYVGPLLI 120
           LTLP + G + +PIVL P KKL DYCDGN++ LT+ FKDLG Q  YR LF  EY+GPL+I
Sbjct: 61  LTLPMQAGIQGKPIVLSPKKKLVDYCDGNVKNLTVVFKDLGVQVLYRTLFFWEYLGPLVI 120

Query: 121 YPMFYYFPVYKFFGYKDEHHIYPAQSYAMYYWCLHYSKRIVETFFVHRFSHGTSPLSNVF 180
           YP+FYYFPVYK+FGY+ E  +YP Q+YAMYYWCLHYSKRI+ETFFVHRFSH TSPLSNVF
Sbjct: 121 YPIFYYFPVYKYFGYEGERVVYPVQTYAMYYWCLHYSKRIMETFFVHRFSHATSPLSNVF 180

Query: 181 RNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGK 240
           RNC YYW+FGA I YYVNHP+YTPVNDLQMKIGFGFGL+CQ+SN YCH++LRNLR+P+G 
Sbjct: 181 RNCAYYWTFGAYIAYYVNHPMYTPVNDLQMKIGFGFGLICQVSNFYCHILLRNLRNPNGN 240

Query: 241 GGYKIPNGFLFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRL 300
           GGY+IP+GFLF+IVTCANYTTEIYQWLGFNIATQT+AGY+F++ A  IMT WA+AKHRRL
Sbjct: 241 GGYQIPHGFLFNIVTCANYTTEIYQWLGFNIATQTIAGYVFLVVAALIMTNWALAKHRRL 300

Query: 301 EKLFDGKEGRPKYPRRWVILPPF 324
           +KLFDGKEGRP+YPRRWVILPPF
Sbjct: 301 KKLFDGKEGRPRYPRRWVILPPF 309

BLAST of Cla97C04G074910 vs. ExPASy TrEMBL
Match: A0A6J1BAL0 (very-long-chain enoyl-CoA reductase OS=Herrania umbratica OX=108875 GN=LOC110425038 PE=3 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 4.3e-141
Identity = 234/323 (72.45%), Postives = 274/323 (84.83%), Query Frame = 0

Query: 1   MKVSILSRSGREIITGGLQIDDSCSRKVSLCDGESKIATVSDLQDAIHQRRRKYYPSRQR 60
           MKV+++SRSGRE+I GGL++ DS              ATV+DLQ+AIH+R +K+YPSRQR
Sbjct: 1   MKVTLVSRSGREVIKGGLELHDS--------------ATVADLQEAIHKRTKKFYPSRQR 60

Query: 61  LTLPAKPGSKERPIVLIPSKKLEDYCDGNLEYLTITFKDLGPQFSYRMLFLLEYVGPLLI 120
           LTLP  PGSKERP+VL   K L+DYCDGN   +T+ FKDLGPQ SYR LF  EY+GPL++
Sbjct: 61  LTLPVPPGSKERPVVLNCKKSLKDYCDGNQNNITVLFKDLGPQVSYRTLFFFEYLGPLIL 120

Query: 121 YPMFYYFPVYKFFGYKDEHHIYPAQSYAMYYWCLHYSKRIVETFFVHRFSHGTSPLSNVF 180
           YP+FYYFPVY+FFGYK E  I+P Q+YA+YYWC HY KRI+ETFF+HRFSH TSPLSNVF
Sbjct: 121 YPIFYYFPVYQFFGYKVERVIHPVQTYALYYWCFHYFKRIMETFFIHRFSHATSPLSNVF 180

Query: 181 RNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGK 240
           RNC YYW+FG+ I YYVNHPLYTPV+DLQMKIGFGFGL+CQ++N YCH+IL+NLR P G 
Sbjct: 181 RNCAYYWTFGSYIAYYVNHPLYTPVSDLQMKIGFGFGLVCQLANSYCHIILKNLRSPDGS 240

Query: 241 GGYKIPNGFLFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRL 300
           GGY+IP GFLF+IVTCANYTTEIYQWLGFNIATQTVAGY+F++ AT IMT WA+AKHRRL
Sbjct: 241 GGYQIPRGFLFNIVTCANYTTEIYQWLGFNIATQTVAGYVFLVVATSIMTNWALAKHRRL 300

Query: 301 EKLFDGKEGRPKYPRRWVILPPF 324
           +KLFDGKEGRPKYPRRWVILPPF
Sbjct: 301 KKLFDGKEGRPKYPRRWVILPPF 309

BLAST of Cla97C04G074910 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 490.3 bits (1261), Expect = 1.2e-138
Identity = 223/323 (69.04%), Postives = 264/323 (81.73%), Query Frame = 0

Query: 1   MKVSILSRSGREIITGGLQIDDSCSRKVSLCDGESKIATVSDLQDAIHQRRRKYYPSRQR 60
           MKV+++SRSGRE++   L + DS              ATV+DLQ+A H+R +K+YPSRQR
Sbjct: 1   MKVTVVSRSGREVLKAPLDLPDS--------------ATVADLQEAFHKRAKKFYPSRQR 60

Query: 61  LTLPAKPGSKERPIVLIPSKKLEDYCDGNLEYLTITFKDLGPQFSYRMLFLLEYVGPLLI 120
           LTLP  PGSK++P+VL   K L++YCDGN   LT+ FKDLG Q SYR LF  EY+GPLLI
Sbjct: 61  LTLPVTPGSKDKPVVLNSKKSLKEYCDGNNNSLTVVFKDLGAQVSYRTLFFFEYLGPLLI 120

Query: 121 YPMFYYFPVYKFFGYKDEHHIYPAQSYAMYYWCLHYSKRIVETFFVHRFSHGTSPLSNVF 180
           YP+FYYFPVYKF GY ++  I+P Q+YAMYYWC HY KRI+ETFFVHRFSH TSP+ NVF
Sbjct: 121 YPVFYYFPVYKFLGYGEDCVIHPVQTYAMYYWCFHYFKRILETFFVHRFSHATSPIGNVF 180

Query: 181 RNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGK 240
           RNC YYWSFGA I YYVNHPLYTPV+DLQMKIGFGFGL+CQ++N YCH++L+NLRDPSG 
Sbjct: 181 RNCAYYWSFGAYIAYYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHILLKNLRDPSGA 240

Query: 241 GGYKIPNGFLFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRL 300
           GGY+IP GFLF+IVTCANYTTEIYQWLGFNIATQT+AGY+F+  A  IMT WA+ KH RL
Sbjct: 241 GGYQIPRGFLFNIVTCANYTTEIYQWLGFNIATQTIAGYVFLAVAALIMTNWALGKHSRL 300

Query: 301 EKLFDGKEGRPKYPRRWVILPPF 324
            K+FDGK+G+PKYPRRWVILPPF
Sbjct: 301 RKIFDGKDGKPKYPRRWVILPPF 309

BLAST of Cla97C04G074910 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 73.2 bits (178), Expect = 4.4e-13
Identity = 55/189 (29.10%), Postives = 86/189 (45.50%), Query Frame = 0

Query: 116 GPLLIYPMFYYFPVYKFFGYKDEHHIYPAQSYAMYYWCLHYSKRIVETFFVHRFSHGTSP 175
           G LL+Y   +      FF    +   +     A+    LH+ KR+ E  F+H++S G + 
Sbjct: 68  GMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSAL---ALHFFKRVFEVLFIHKYSGGMA- 127

Query: 176 LSNVFRNCMYYWSFGALIGYYVNHPLYTPVNDLQMKI-GFGFGLLCQISNLYCHLILRNL 235
           + +       Y+S  AL+ Y  N  L        MK+ G    ++  + NLY H++L  L
Sbjct: 128 IDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLLAKL 187

Query: 236 RDPSGKGGYKIPNGFLFSIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAA-------TY 295
           R   GK  YKIP G LF I+ C +Y  EI  +  F + +QT+  + F M         +Y
Sbjct: 188 RKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIGRSY 247

Query: 296 IMTKWAIAK 297
               W ++K
Sbjct: 248 ATRTWYLSK 252

BLAST of Cla97C04G074910 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 46.2 bits (108), Expect = 5.8e-05
Identity = 33/114 (28.95%), Postives = 54/114 (47.37%), Query Frame = 0

Query: 214 FGFGLLCQISNLYCHL----ILRNLRDPSGKGGYKIPNGFLFSIVTCANYTTEIYQWLGF 273
           F  G++  I+ +Y ++     L  L+    +GGY IP G  F +V+C NY  E  +WLG+
Sbjct: 153 FVIGMVVFITGMYINITSDRTLVRLK-KENRGGYVIPRGGWFELVSCPNYFGEAIEWLGW 212

Query: 274 NIATQTVAGYIFVMAATYIMTKWAIAKHRRLEKLFDGKEGRPKYPRRWVILPPF 324
            + T + AG  F +     +   A A H+     F     + +YP+    + PF
Sbjct: 213 AVMTWSWAGIGFFLYTCSNLFPRARASHKWYIAKF-----KEEYPKTRKAVIPF 260

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038877634.18.5e-16889.20very-long-chain enoyl-CoA reductase-like [Benincasa hispida][more]
XP_022143915.14.1e-16285.49very-long-chain enoyl-CoA reductase-like [Momordica charantia][more]
KAE7996787.12.1e-14273.99hypothetical protein FH972_001478 [Carpinus fangiana][more]
TXG58604.12.8e-14274.30hypothetical protein EZV62_016433 [Acer yangbiense][more]
XP_042954556.11.0e-14173.37very-long-chain enoyl-CoA reductase-like [Carya illinoinensis] >KAG2730255.1 hyp... [more]
Match NameE-valueIdentityDescription
Q9M2U21.6e-13769.04Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana OX=3702 GN=ECR PE=1 ... [more]
Q55C173.2e-5341.72Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn... [more]
Q9NZ014.3e-4538.22Very-long-chain enoyl-CoA reductase OS=Homo sapiens OX=9606 GN=TECR PE=1 SV=1[more]
Q642327.3e-4539.12Very-long-chain enoyl-CoA reductase OS=Rattus norvegicus OX=10116 GN=Tecr PE=1 S... [more]
Q9CY279.5e-4539.12Very-long-chain enoyl-CoA reductase OS=Mus musculus OX=10090 GN=Tecr PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CQ632.0e-16285.49very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A5N6QC861.0e-14273.99S5A_REDUCTASE domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_... [more]
A0A5C7HP471.3e-14274.30S5A_REDUCTASE domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_0... [more]
M0T4A41.5e-14172.45S5A_REDUCTASE domain-containing protein OS=Musa acuminata subsp. malaccensis OX=... [more]
A0A6J1BAL04.3e-14172.45very-long-chain enoyl-CoA reductase OS=Herrania umbratica OX=108875 GN=LOC110425... [more]
Match NameE-valueIdentityDescription
AT3G55360.11.2e-13869.043-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT5G16010.14.4e-1329.103-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT2G38050.15.8e-0528.953-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPFAMPF02544Steroid_dhcoord: 173..323
e-value: 6.1E-27
score: 94.5
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPROSITEPS50244S5A_REDUCTASEcoord: 213..294
score: 20.984463
IPR0393573-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductasePANTHERPTHR105563-OXO-5-ALPHA-STEROID 4-DEHYDROGENASEcoord: 35..324
NoneNo IPR availablePANTHERPTHR10556:SF28SC2coord: 35..324
NoneNo IPR availableCDDcd01801Ubl_TECR_likecoord: 2..100
e-value: 9.34248E-16
score: 69.2375

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C04G074910.1Cla97C04G074910.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042761 very long-chain fatty acid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003865 3-oxo-5-alpha-steroid 4-dehydrogenase activity
molecular_function GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors