ClCG11G001040 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG11G001040
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	rRNA N-glycosidase
Location	CG_Chr11: 1318242 .. 1319059 (+)
RNA-Seq Expression	ClCG11G001040
Synteny	ClCG11G001040

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATAGATTATCAACGCTTTCACTGCTAGTTCTAGCCATCTTCCTTGGAGGTTCATCCTTCACTGCAGGCGATGTAAGTTTCAGTTTATCCGGTTCGACTTCCTCGTCTTACAGCAAGTTCATCAAAGATCTGAGGAACGCTCTTCCGTCGAAGGCCAAAGTATACAATATAACTCTACTACTCAGCTCCGCCACCGGCGCAAGTCGCTACACACTAATGAAACTCTCCAACTATGACGGTAAAACCATAACAGTTGCTATAGATGTAACAAACGTCTATATCATGGGGTATCTCGTCAACACAACTTCCTACTTTTTCAACGAACCTGATGCTAAATTAGCTTCTCAACATGTATTCAAAGGCAGATTATTACACTTCCATACTCTGGCAATTACGAGAGGCTTCAAATTGCTGCAGGCAAAAACAGAGAGAGAATTCCTCTTGGATTTCGAGCTTTGGACAGTGCAATTACAAGCTTGTTTCATTATGATTCCACAACTTCTGCTGCAGCATTTCTTGTGATAATTCAGTCCACAGCTGAGGCATCAAGATATAAATATATTGAAGGTCAAATTATTGAGAGAATTCATAAAGATGAGGTTCCAAGTTTGGCTACTATTAGTTTAGAAAATAGTTGGTCTGCTTTATCTAAGCAGATTCAATTGGCACAAACTAATAATGGAAACTTTAAAACTCCTGTTGGGATTATAGATAATCAAGGCAAACGAGTTGAAATAACCAATGTGAATTCGAAAGTTGTGAGTAACAATATCAAGTTGCTGCTAAACTCACAAAATATTGCAATGAGGCCATGA

mRNA sequence

ATGAATAGATTATCAACGCTTTCACTGCTAGTTCTAGCCATCTTCCTTGGAGGTTCATCCTTCACTGCAGGCGATGTAAGTTTCAGTTTATCCGGTTCGACTTCCTCGTCTTACAGCAAGTTCATCAAAGATCTGAGGAACGCTCTTCCGTCGAAGGCCAAAGTATACAATATAACTCTACTACTCAGCTCCGCCACCGGCGCAAGTCGCTACACACTAATGAAACTCTCCAACTATGACGGTAAAACCATAACAGTTGCTATAGATGTAACAAACGTCTATATCATGGGGTATCTCGTCAACACAACTTCCTACTTTTTCAACGAACCTGATGCTAAATTAGCTTCTCAACATAGGCTTCAAATTGCTGCAGGCAAAAACAGAGAGAGAATTCCTCTTGGATTTCGAGCTTTGGACAGTGCAATTACAAGCTTGTTTCATTATGATTCCACAACTTCTGCTGCAGCATTTCTTGTGATAATTCAGTCCACAGCTGAGGCATCAAGATATAAATATATTGAAGGTCAAATTATTGAGAGAATTCATAAAGATGAGGTTCCAAGTTTGGCTACTATTAGTTTAGAAAATAGTTGGTCTGCTTTATCTAAGCAGATTCAATTGGCACAAACTAATAATGGAAACTTTAAAACTCCTGTTGGGATTATAGATAATCAAGGCAAACGAGTTGAAATAACCAATGTGAATTCGAAAGTTGTGAGTAACAATATCAAGTTGCTGCTAAACTCACAAAATATTGCAATGAGGCCATGA

Coding sequence (CDS)

ATGAATAGATTATCAACGCTTTCACTGCTAGTTCTAGCCATCTTCCTTGGAGGTTCATCCTTCACTGCAGGCGATGTAAGTTTCAGTTTATCCGGTTCGACTTCCTCGTCTTACAGCAAGTTCATCAAAGATCTGAGGAACGCTCTTCCGTCGAAGGCCAAAGTATACAATATAACTCTACTACTCAGCTCCGCCACCGGCGCAAGTCGCTACACACTAATGAAACTCTCCAACTATGACGGTAAAACCATAACAGTTGCTATAGATGTAACAAACGTCTATATCATGGGGTATCTCGTCAACACAACTTCCTACTTTTTCAACGAACCTGATGCTAAATTAGCTTCTCAACATAGGCTTCAAATTGCTGCAGGCAAAAACAGAGAGAGAATTCCTCTTGGATTTCGAGCTTTGGACAGTGCAATTACAAGCTTGTTTCATTATGATTCCACAACTTCTGCTGCAGCATTTCTTGTGATAATTCAGTCCACAGCTGAGGCATCAAGATATAAATATATTGAAGGTCAAATTATTGAGAGAATTCATAAAGATGAGGTTCCAAGTTTGGCTACTATTAGTTTAGAAAATAGTTGGTCTGCTTTATCTAAGCAGATTCAATTGGCACAAACTAATAATGGAAACTTTAAAACTCCTGTTGGGATTATAGATAATCAAGGCAAACGAGTTGAAATAACCAATGTGAATTCGAAAGTTGTGAGTAACAATATCAAGTTGCTGCTAAACTCACAAAATATTGCAATGAGGCCATGA

Protein sequence

MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITLLLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQHRLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQSTAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIIDNQGKRVEITNVNSKVVSNNIKLLLNSQNIAMRP

Homology

[Close]

BLAST of ClCG11G001040 vs. NCBI nr
Match: XP_022953306.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita moschata])

HSP 1 Score: 345.1 bits (884), Expect = 5.1e-91
Identity = 192/270 (71.11%), Postives = 221/270 (81.85%), Query Frame = 0


Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           MN +S   L++LAIF G SS  AGDVSF+L GS S SYS FI+ LR+ALPSK KVYNI+L
Sbjct: 11  MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 70

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQH-- 120
           LL SATGASRYT +KLSNYDGK ITVAIDVTNVYIMGYLVN+TSYFFNE DA+LAS++  
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 ---------------RLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQS 180
                          +LQ AAGK RE+IPLGF ALD+AIT+LFHYDST +AAAF+V+IQ 
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIID 240
           TAEASRY+YIE Q+I+RI KD+VPSLA ISLENSWSALSKQIQLAQTN G FK PV I D
Sbjct: 191 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 NQGKRVEITNVNSKVVSNNIKLLLNSQNIA 254
           +QG+RVEITNV+SKVV+ NI+LLLNS+NIA
Sbjct: 251 DQGQRVEITNVSSKVVAKNIQLLLNSKNIA 280

[Close]

BLAST of ClCG11G001040 vs. NCBI nr
Match: XP_022992350.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita maxima])

HSP 1 Score: 345.1 bits (884), Expect = 5.1e-91
Identity = 194/270 (71.85%), Postives = 219/270 (81.11%), Query Frame = 0


Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           MN +S   L++LAIF G SS  AGDVSF+L GS S SYS FIK LR+ALPSK KVYNI+L
Sbjct: 11  MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 70

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQH-- 120
           LL SATGASRYT +KLSNYDGK ITVAIDVTNVYIMGYLVN+TSYFFNE DA+LAS++  
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 ---------------RLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQS 180
                          +LQ AAGK RE+IPLGF ALDSAIT+LFHYDST +AAAF+V+IQ 
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIID 240
           TAEASRYKYIE Q+I+RI KD+VPS A ISLENSWSALSKQIQLAQTN G FK PV I D
Sbjct: 191 TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 NQGKRVEITNVNSKVVSNNIKLLLNSQNIA 254
           +QG+RVEITNV+S VV+ NI+LLLNSQNIA
Sbjct: 251 DQGQRVEITNVSSNVVTKNIQLLLNSQNIA 280

[Close]

BLAST of ClCG11G001040 vs. NCBI nr
Match: KAG6575376.1 (hypothetical protein SDJN03_26015, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013917.1 hypothetical protein SDJN02_24086, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 345.1 bits (884), Expect = 5.1e-91
Identity = 192/270 (71.11%), Postives = 221/270 (81.85%), Query Frame = 0


Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           MN +S   L++LAIF G SS  AGDVSF+L GS S SYS FI+ LR+ALPSK KVYNI+L
Sbjct: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQH-- 120
           LL SATGASRYT +KLSNYDGK ITVAIDVTNVYIMGYLVN+TSYFFNE DA+LAS++  
Sbjct: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120

Query: 121 ---------------RLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQS 180
                          +LQ AAGK RE+IPLGF ALD+AIT+LFHYDST +AAAF+V+IQ 
Sbjct: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 180

Query: 181 TAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIID 240
           TAEASRY+YIE Q+I+RI KD+VPSLA ISLENSWSALSKQIQLAQTN G FK PV I D
Sbjct: 181 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240

Query: 241 NQGKRVEITNVNSKVVSNNIKLLLNSQNIA 254
           +QG+RVEITNV+SKVV+ NI+LLLNS+NIA
Sbjct: 241 DQGQRVEITNVSSKVVAKNIQLLLNSKNIA 270

[Close]

BLAST of ClCG11G001040 vs. NCBI nr
Match: Q00465.1 (RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Luffa aegyptiaca] >CAA44229.1 a-luffin [Luffa aegyptiaca])

HSP 1 Score: 344.7 bits (883), Expect = 6.7e-91
Identity = 195/268 (72.76%), Postives = 221/268 (82.46%), Query Frame = 0


Query: 4   LSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITLLLS 63
           +   ++L+LAIF+  S+  A DV FSLSGS+S+SYSKFI DLR ALPS   VYNITLLLS
Sbjct: 1   MKRFTVLILAIFVAASTVEA-DVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLS 60

Query: 64  SATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQH----- 123
           SA+GASRYTLM LSNYDGK ITVA+DVTNVYIMGYLVN+TSYFFNE DAKLASQ+     
Sbjct: 61  SASGASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGS 120

Query: 124 ------------RLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQSTAE 183
                       +LQ AAGK RE+IPLGF ALDSAIT+LFHYDST +AAAFLVIIQ+TAE
Sbjct: 121 TIVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAE 180

Query: 184 ASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIIDNQG 243
           ASR+KYIEGQIIERI K++VPSLATISLEN WSALSKQIQLAQTNNG FKTPV I D++G
Sbjct: 181 ASRFKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKG 240

Query: 244 KRVEITNVNSKVVSNNIKLLLN-SQNIA 254
           +RVEITNV SKVV+ NI+LLLN  QN+A
Sbjct: 241 QRVEITNVTSKVVTKNIQLLLNYKQNVA 267

[Close]

BLAST of ClCG11G001040 vs. NCBI nr
Match: XP_023520860.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 344.0 bits (881), Expect = 1.1e-90
Identity = 191/270 (70.74%), Postives = 221/270 (81.85%), Query Frame = 0


Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           MN +S   L++LAIF G SS  AGDVSF+L GS S SYS FI+ LR+ALPSK KVYNI+L
Sbjct: 11  MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 70

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQH-- 120
           L+ SATGASRYT +KLSNYDGK ITVAIDVTNVYIMGYLVN+TSYFFNE DA+LAS++  
Sbjct: 71  LVGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 ---------------RLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQS 180
                          +LQ AAGK RE+IPLGF ALD+AIT+LFHYDST +AAAF+V+IQ 
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFMVVIQC 190

Query: 181 TAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIID 240
           TAEASRY+YIE Q+I+RI KD+VPSLA ISLENSWSALSKQIQLAQTN G FK PV I D
Sbjct: 191 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 NQGKRVEITNVNSKVVSNNIKLLLNSQNIA 254
           +QG+RVEITNV+SKVV+ NI+LLLNS+NIA
Sbjct: 251 DQGQRVEITNVSSKVVAKNIQLLLNSKNIA 280

[Close]

BLAST of ClCG11G001040 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 8.7e-94
Identity = 195/268 (72.76%), Postives = 221/268 (82.46%), Query Frame = 0


Query: 4   LSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITLLLS 63
           +   ++L+LAIF+  S+  A DV FSLSGS+S+SYSKFI DLR ALPS   VYNITLLLS
Sbjct: 1   MKRFTVLILAIFVAASTVEA-DVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLS 60

Query: 64  SATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQH----- 123
           SA+GASRYTLM LSNYDGK ITVA+DVTNVYIMGYLVN+TSYFFNE DAKLASQ+     
Sbjct: 61  SASGASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGS 120

Query: 124 ------------RLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQSTAE 183
                       +LQ AAGK RE+IPLGF ALDSAIT+LFHYDST +AAAFLVIIQ+TAE
Sbjct: 121 TIVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAE 180

Query: 184 ASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIIDNQG 243
           ASR+KYIEGQIIERI K++VPSLATISLEN WSALSKQIQLAQTNNG FKTPV I D++G
Sbjct: 181 ASRFKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKG 240

Query: 244 KRVEITNVNSKVVSNNIKLLLN-SQNIA 254
           +RVEITNV SKVV+ NI+LLLN  QN+A
Sbjct: 241 QRVEITNVTSKVVTKNIQLLLNYKQNVA 267

[Close]

BLAST of ClCG11G001040 vs. ExPASy Swiss-Prot
Match: P84530 (Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=2)

HSP 1 Score: 328.9 bits (842), Expect = 5.0e-89
Identity = 184/241 (76.35%), Postives = 201/241 (83.40%), Query Frame = 0


Query: 25  DVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITLLLSSATGASRYTLMKLSNYDGKTI 84
           DVSFSLSGS+S+SYSKFI  LR ALPS   VYNITLLLSSA+GASRYTLMKLSNYDGK I
Sbjct: 1   DVSFSLSGSSSTSYSKFIGALRKALPSNGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 60

Query: 85  TVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQH-----------------RLQIAAGKN 144
           TVAIDVTNVYIMGYLVN+TSYFFNE DAKLASQ+                 +LQ AAGK 
Sbjct: 61  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKI 120

Query: 145 RERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQSTAEASRYKYIEGQIIERIHKDEVP 204
           RE+IPLGF ALDSAIT+LFHYDST +AAAFLVIIQ+TAE+SR+KYIEGQII RI K+ VP
Sbjct: 121 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNGVP 180

Query: 205 SLATISLENSWSALSKQIQLAQTNNGNFKTPVGIIDNQGKRVEITNVNSKVVSNNIKLLL 249
           SLATISLEN WSALSKQIQLAQTNNG FKTPV I+D  G+RVEI NV SKVV+ NI+LLL
Sbjct: 181 SLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQLLL 240

[Close]

BLAST of ClCG11G001040 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 328.6 bits (841), Expect = 6.5e-89
Identity = 182/271 (67.16%), Postives = 213/271 (78.60%), Query Frame = 0


Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           M+R S LS L+LAIFLGG S   GDVSF LSG+   SY  FIKDLRNALP + KVYNI L
Sbjct: 1   MSRFSVLSFLILAIFLGG-SIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPL 60

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQH-- 120
           LL S +GA RY LM L NYDGKTITVA+DVTNVYIMGYL +TTSYFFNEP A+LASQ+  
Sbjct: 61  LLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVF 120

Query: 121 ----------------RLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQ 180
                           RLQIAAGK RE+IP+G  ALDSAI++L HYDST +A A LV+IQ
Sbjct: 121 RDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQ 180

Query: 181 STAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGII 240
           +TAEA+R+KYIE QI ER ++DEVPSLATISLENSWS LSKQIQLAQ NNG F+TP+ ++
Sbjct: 181 TTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLV 240

Query: 241 DNQGKRVEITNVNSKVVSNNIKLLLNSQNIA 254
           DN+G RV+ITNV SKVV++NI+LLLN++NIA
Sbjct: 241 DNKGNRVQITNVTSKVVTSNIQLLLNTRNIA 270

[Close]

BLAST of ClCG11G001040 vs. ExPASy Swiss-Prot
Match: P22851 (Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 4.1e-83
Identity = 179/249 (71.89%), Postives = 200/249 (80.32%), Query Frame = 0


Query: 25  DVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITLLLSSATGASRYTLMKLSNYDGKTI 84
           +VSFSLSG+ S SYSKFI  LR ALPSK KV NI LLL SA+GASRY LM+LSNYD K I
Sbjct: 2   NVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKAI 61

Query: 85  TVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQH-----------------RLQIAAGKN 144
           T+AIDVTNVYIMGYLVN+TSYF NE DAKLASQ+                 RLQ AAGK 
Sbjct: 62  TMAIDVTNVYIMGYLVNSTSYFANESDAKLASQYVFKGSTLVTIPYSGNYERLQNAAGKI 121

Query: 145 RERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQSTAEASRYKYIEGQIIERIHKDEVP 204
           RE+IPLGFRALDSA+TS+FHYDST +AAAFLVI+Q+TAEASR+KYIEGQIIERI K+EVP
Sbjct: 122 REKIPLGFRALDSALTSIFHYDSTAAAAAFLVILQTTAEASRFKYIEGQIIERIPKNEVP 181

Query: 205 SLATISLEN-SWSALSKQIQLAQTNNGNFKTPVGIIDNQGKRVEITNVNSKVVSN--NIK 254
           S A +SLEN +WS LSKQIQLAQTNNG F+TPV IIDN+G+RVEITN+ SKV     N K
Sbjct: 182 SPAALSLENEAWSLLSKQIQLAQTNNGAFRTPVVIIDNKGQRVEITNLASKVQIKDVNSK 241

[Close]

BLAST of ClCG11G001040 vs. ExPASy Swiss-Prot
Match: Q9FRX4 (Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 8.5e-73
Identity = 157/272 (57.72%), Postives = 194/272 (71.32%), Query Frame = 0


Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVS--FSLSGSTSSSYSKFIKDLRNALPSKAKVYNI 60
           MNR S L  LV+     G     GDV+  FSL GS   SYSKFI  +RNALP+   +YNI
Sbjct: 1   MNRFSVLMCLVILSIFHGVPTAEGDVTVKFSLLGSNHKSYSKFITSMRNALPNAGDIYNI 60

Query: 61  TLLLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQ- 120
            LL+ S +G+ RY LM+LSNY+G TIT+A+DVTNVYIMGYLVN TSYFFNE DA+LAS+ 
Sbjct: 61  PLLVPSISGSRRYILMQLSNYEGNTITMAVDVTNVYIMGYLVNGTSYFFNETDAQLASKF 120

Query: 121 ----------------HRLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVII 180
                            +LQ  A K R+ IPLGF ALDSAI++L++YDS ++  AFLV+I
Sbjct: 121 VFQGTKSITLPYSGNYQKLQSVARKERDSIPLGFMALDSAISTLYYYDSRSAPIAFLVLI 180

Query: 181 QSTAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGI 240
           Q+TAEA+RYKYIE QII+RI   +VP LA ISLEN WS LSKQIQ+A++NNG F+TPV I
Sbjct: 181 QTTAEAARYKYIEKQIIDRISVSKVPDLAAISLENEWSLLSKQIQIAKSNNGQFQTPVKI 240

Query: 241 IDNQGKRVEITNVNSKVVSNNIKLLLNSQNIA 254
           I+++G   E+TNV+S VV+ NI LLLN  NIA
Sbjct: 241 INDKGILTEVTNVSSLVVTKNIMLLLNKLNIA 272

[Close]

BLAST of ClCG11G001040 vs. ExPASy TrEMBL
Match: A0A6J1JVG7 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 2.5e-91
Identity = 194/270 (71.85%), Postives = 219/270 (81.11%), Query Frame = 0


Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           MN +S   L++LAIF G SS  AGDVSF+L GS S SYS FIK LR+ALPSK KVYNI+L
Sbjct: 11  MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 70

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQH-- 120
           LL SATGASRYT +KLSNYDGK ITVAIDVTNVYIMGYLVN+TSYFFNE DA+LAS++  
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 ---------------RLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQS 180
                          +LQ AAGK RE+IPLGF ALDSAIT+LFHYDST +AAAF+V+IQ 
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIID 240
           TAEASRYKYIE Q+I+RI KD+VPS A ISLENSWSALSKQIQLAQTN G FK PV I D
Sbjct: 191 TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 NQGKRVEITNVNSKVVSNNIKLLLNSQNIA 254
           +QG+RVEITNV+S VV+ NI+LLLNSQNIA
Sbjct: 251 DQGQRVEITNVSSNVVTKNIQLLLNSQNIA 280

[Close]

BLAST of ClCG11G001040 vs. ExPASy TrEMBL
Match: A0A6J1GMM2 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 2.5e-91
Identity = 192/270 (71.11%), Postives = 221/270 (81.85%), Query Frame = 0


Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           MN +S   L++LAIF G SS  AGDVSF+L GS S SYS FI+ LR+ALPSK KVYNI+L
Sbjct: 11  MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 70

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQH-- 120
           LL SATGASRYT +KLSNYDGK ITVAIDVTNVYIMGYLVN+TSYFFNE DA+LAS++  
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 ---------------RLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQS 180
                          +LQ AAGK RE+IPLGF ALD+AIT+LFHYDST +AAAF+V+IQ 
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIID 240
           TAEASRY+YIE Q+I+RI KD+VPSLA ISLENSWSALSKQIQLAQTN G FK PV I D
Sbjct: 191 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 NQGKRVEITNVNSKVVSNNIKLLLNSQNIA 254
           +QG+RVEITNV+SKVV+ NI+LLLNS+NIA
Sbjct: 251 DQGQRVEITNVSSKVVAKNIQLLLNSKNIA 280

[Close]

BLAST of ClCG11G001040 vs. ExPASy TrEMBL
Match: B0EVM6 (rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.5e-88
Identity = 193/270 (71.48%), Postives = 215/270 (79.63%), Query Frame = 0


Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           MNR + LSLL+  I +   +    +VSFSLSG+ S SYSKFI  LR ALPSK KV NI L
Sbjct: 1   MNRFTFLSLLI--ILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQH-- 120
           LL SA+GASRY LM+LSNYD K IT+AIDVTNVYIMGYLVN+TSYFFNE DAKLASQ+  
Sbjct: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 ---------------RLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQS 180
                          RLQ AAGK RE+IPLGFRA DSAITSLFHYDST +A AFLVIIQ+
Sbjct: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180

Query: 181 TAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIID 240
           TAEASR+KYIEGQIIERI K+EVPS A +SLEN WSALSKQIQLAQTNNG F+TPV IID
Sbjct: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240

Query: 241 NQGKRVEITNVNSKVVSNNIKLLLNSQNIA 254
           N+G+RVEI +VNSKVV+NNIKLLLN QNIA
Sbjct: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIA 268

[Close]

BLAST of ClCG11G001040 vs. ExPASy TrEMBL
Match: Q00980 (rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.5e-88
Identity = 193/270 (71.48%), Postives = 215/270 (79.63%), Query Frame = 0


Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           MNR + LSLL+  I +   +    +VSFSLSG+ S SYSKFI  LR ALPSK KV NI L
Sbjct: 1   MNRFTFLSLLI--ILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQH-- 120
           LL SA+GASRY LM+LSNYD K IT+AIDVTNVYIMGYLVN+TSYFFNE DAKLASQ+  
Sbjct: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 ---------------RLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQS 180
                          RLQ AAGK RE+IPLGFRA DSAITSLFHYDST +A AFLVIIQ+
Sbjct: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180

Query: 181 TAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIID 240
           TAEASR+KYIEGQIIERI K+EVPS A +SLEN WSALSKQIQLAQTNNG F+TPV IID
Sbjct: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240

Query: 241 NQGKRVEITNVNSKVVSNNIKLLLNSQNIA 254
           N+G+RVEI +VNSKVV+NNIKLLLN QNIA
Sbjct: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIA 268

[Close]

BLAST of ClCG11G001040 vs. ExPASy TrEMBL
Match: A0A6J1DSN8 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023551 PE=3 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 2.4e-86
Identity = 182/271 (67.16%), Postives = 213/271 (78.60%), Query Frame = 0


Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           M+R S LS L+LAIFLGG S   GDVSF LSG+   SY  FIKDLRNALP + KVYNI L
Sbjct: 1   MSRFSVLSFLILAIFLGG-SIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPL 60

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQH-- 120
           LL S +GA RY LM L NYDGKTITVA+DVTNVYIMGYL +TTSYFFNEP A+LASQ+  
Sbjct: 61  LLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVF 120

Query: 121 ----------------RLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQ 180
                           RLQIAAGK RE+IP+G  ALDSAI++L HYDST +A A LV+IQ
Sbjct: 121 RDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQ 180

Query: 181 STAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGII 240
           +TAEA+R+KYIE QI ER ++DEVPSLATISLENSWS LSKQIQLAQ NNG F+TP+ ++
Sbjct: 181 TTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLV 240

Query: 241 DNQGKRVEITNVNSKVVSNNIKLLLNSQNIA 254
           DN+G RV+ITNV SKVV++NI+LLLN++NIA
Sbjct: 241 DNKGNRVQITNVTSKVVTSNIQLLLNTRNIA 270

The following BLAST results are available for this feature:

BLAST of ClCG11G001040 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022953306.1	5.1e-91	71.11	ribosome-inactivating protein luffaculin 1-like [Cucurbita moschata]	[more]
XP_022992350.1	5.1e-91	71.85	ribosome-inactivating protein luffaculin 1-like [Cucurbita maxima]	[more]
KAG6575376.1	5.1e-91	71.11	hypothetical protein SDJN03_26015, partial [Cucurbita argyrosperma subsp. sorori...	[more]
Q00465.1	6.7e-91	72.76	RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-g...	[more]
XP_023520860.1	1.1e-90	70.74	ribosome-inactivating protein luffaculin 1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of ClCG11G001040 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q00465	8.7e-94	72.76	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P84530	5.0e-89	76.35	Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=...	[more]
P16094	6.5e-89	67.16	Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=...	[more]
P22851	4.1e-83	71.89	Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
Q9FRX4	8.5e-73	57.72	Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1	[more]

BLAST of ClCG11G001040 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JVG7	2.5e-91	71.85	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1	[more]
A0A6J1GMM2	2.5e-91	71.11	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1	[more]
B0EVM6	1.5e-88	71.48	rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1	[more]
Q00980	1.5e-88	71.48	rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
A0A6J1DSN8	2.4e-86	67.16	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023551 PE=3 SV=1	[more]

BLAST of ClCG11G001040 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 36..49 score: 46.05 coord: 189..206 score: 53.22 coord: 157..177 score: 55.26
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 34..206 e-value: 5.1E-23 score: 81.7
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 26..251
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 24..168 e-value: 5.0E-33 score: 116.0
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 169..253 e-value: 7.9E-28 score: 98.2
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 26..251
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 161..177
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 25..251

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG11G001040.1	ClCG11G001040.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051607	defense response to virus
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity