ClCG08G018080 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG08G018080
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Descriptiondual-specificity RNA methyltransferase RlmN
LocationCG_Chr08: 30085663 .. 30088806 (+)
RNA-Seq ExpressionClCG08G018080
SyntenyClCG08G018080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAACAGAAGAACCCTCGATGGTTTGGATTAGGCGCATGTTCTTCCCAAAGCCTTCCATGGCCTCCTCCATACTTGCCCCTTCGCACTTCCATTCCCTTTCTGTTCCTTCTGCACGCCTATCGCCACCATTGCACTTAATTTCTTTCCTCTCTACTGTTGGTCGCCCGTTTACGACTACCTCTTGCACATCACCTACGCCGTTATTTGCTCTTTACGATTCCTCACCTGATTTCTGTCTGTTCCTGAGTATATACTTGTCTTTTTACGTTCTTCTACTCTAATTTTTTTACTAGACTCTATCCTTTCTTCAATTCTGCGCTCATTTAGTTGAATGATACTTCTTGCGATCTCTTTTCTTGTAGCTATGCCTGAGGAAAAAGAAATCTTGAACAGTGATTCCGACTCCAAGATGCTTCTAAAGGGTCTGTCTTACACCGAACTTCAGGCAAGTCTCTGCTAAACTCTCCTTTTCTCCTTTTCACTTATTTGTCTCTTCTTCTTCCCCGCTTAATGAAGAAAACCCTTTGCTCTGCTACACGAATGCTAATTTTTGTTTTTACTCTAACATATACGCCGATAGAAATGGGTTCAAGCACGAGGATACAGACCTGGACAAGCTTTAATGCTGTGGAAACGTCTTTATGGGGATAACATTTGGGCTCATACCAGCGATGAATTAGAAGGTCTTCTTGCTTTCTCAATTTATTTGGAACACTAGCTATTGTTTGGAGTTAACGCTTTCATTGATAGCCTATTTTTTCCTTATCTGCTTTCCTCTTGTTGTAACTCGTAACTTATCTACTCTATCCAATGACTTTTTCCGTGCTATAAAATCTTAGGTTTGAACAAAGATTTTAAGAAAATGTTGATTGAAAACGCTGAGTTCAGGGCGCTGTCTCTGAGAGAAATTCTCCCTTCATCTGATGGAACGAGGAAGGTCTGTATAGTTACATTCTAGCAAGTGCTGTTATAATCATTCAATTCTCTTTACTTCTTGTTTCTTTGTGTGCATTGTTCTTTCCGTTAGTTCTTTGGGTTTGTGCTCATAAAGATTCGTCTGATTAATTTTAAAGCTCACAGGATATTACAGATTTTGTTCAATCTGGAAGATGGATTGGTAATAGAAACTGTTGTTATACCTTGCGATAGAGGCAGGACTACCGTTTGCGTTTCTAGTCAAGTGGGCTGTGCCATGAACTGTCAATTCTGCTACACTGGCAGGCAAGCTTCAATTCGGTTAGATGTTTTCAGCATAGTCTTAGTTGAAGTAAACAAGAATGATCAGATTGTTAGTTTTTCATTCAATTTTTCAGGATGGGCCTGAAGAGACATCTGACTGCTGCTGAGATAGTAGAACAGGCAGTTTTTGTGAGGCGTTTGCTTACTAGTGAAGTGGGTTTAATTACTAATGTCGTGTTTATGGTATGTTTAAGATCTGAACTATGTTTTAACATACAAGAGGAAGAATGAAATGTATTGAAACATGCCTTATGTTTCTGGTTTATTTACTAAGCAAGATTTTGTACTGGTAGCAAATAAGGAGATTTTAAAATCCAAAAGGAAAGAATATGGTTGGTAAGAATGAGGATGGAAGTAACATTTTATCGATGTTGGTCCTGGTTGGTTTGGCACTCATACACAAGAATGATTCTACCTTCTTTCAATCTGTTGTGCCTGCAACGTTCTCTCGTTCTCTCGTTTGTAGACTATTCCAAGCTACAAGTTCTTCATGCATTTAGCTGGAACTACAAGTTCTCCATGCATTTAGCTGGAACAAGATTTGCAAACATTTCTTTCTTTTGTAGTTTTCTTTCCTGATAAATTTCGATTGTTTCTGTCTTTCAGCCATATTGAAGATTTATGTTGGGGTTTTGGGTCTTACTGGATACCTATTCTCTTCCTTGTTTCCATTGTTAAAGCCTTTTGTGACATTTATGATGGTGTTGGTCTCGGCGAGAACCATAATCCTTTTCCATGCGTTGATCAGAAAAACTGAAATTGGTTGTTAGGGAATGGGAGAGCCGCTTCACAACATTGACAATGTCATTAAAGCTGCAAATATAATGGTTCATGAACAAGGCCTTCATTTCAGTCCTCGCAAGGTCACTGTTTCAACCAGTGGACTTGTTCCCCAGCTGAAACGTTTCCTGAATGAATGTAACTGTGCTTTAGCTGTTAGTTTGAATGCAACTACTGATGAGGTACACGCTCGTGGTTGGCATTACTCAAATGCTTGAAATTAAAAAATGTGAAGAATTCCCTTCTCTCACATGGGAAAATGAATGTTGCAGGTCAGGAATTGGATCATGCCAATAAACCGGAAGTATAAGTTAGGCTTGCTTCTTCAGACTTTACGTGAGGAACTTCGCTGCAAACACAATTACAAGGTTCTTTTTGAATATGTGATGCTTGCTGGGGTTAATGACAGGTATATTACTGCTCCTATGCTTTGGACTTGATCATTGTTTAAACTGGAGCTGTAGTTGTTGATTGTATCAATTTTTTCCTTTACAATCATTTCTGATCTTTTGTTTTATTTTTACATGCAGCATTGAAGATGCGAACAGGATCGTTGATCTTGTCCAGGGTATTCCATGCAAGATTAACCTTATTTCATTTAATCCTCATTGTGGATCCCAATTTAGACCTACCTGCAAGGAGAAGATGATTGAGTTTCGAAATGTTTTGGCTGAAGCCGGGTTGACTGTTTTCTTGCGACTAAGTAGAGGTGATGACCAGATGGCTGCCTGTGGTCAGTTAGGCAAACCTGGTACTGTTCAAGCTCCTTTACTCCGTGTACCAGATCAATTCCAAATGGCAATGAAATTGTGTCCCTAGTTCCTACGACAAGGTATCAAAAACGTCGACATCACTCCATCGATGTGGCATAAAATGGTGTGCCTAATCATGCAATGGTTAGAACTTATGCCCGTCTTCAATTTTTCAAGTCTTCTCGGAACGGAAGCTGTGGCCCTAGATCGAGAAATTCATCCCTCGTGTGTTCCTAAATTTATTTTCATTCTTCAGTTTTTCTTTTCAACTTGTACCCTTGTTTTTGGGATAAATTTTCGAATTGGTATTTAGAACTAGTTGTATTTCGGTGATGTGGACGTCGTGTGTTCGACTAACTTGAACTTGAC

mRNA sequence

AAAAAAACAGAAGAACCCTCGATGGTTTGGATTAGGCGCATGTTCTTCCCAAAGCCTTCCATGGCCTCCTCCATACTTGCCCCTTCGCACTTCCATTCCCTTTCTGTTCCTTCTGCACGCCTATCGCCACCATTGCACTTAATTTCTTTCCTCTCTACTGTTGGTCGCCCGTTTACGACTACCTCTTGCACATCACCTACGCCGTTATTTGCTCTTTACGATTCCTCACCTGATTTCTGTCTGTTCCTGACTATGCCTGAGGAAAAAGAAATCTTGAACAGTGATTCCGACTCCAAGATGCTTCTAAAGGGTCTGTCTTACACCGAACTTCAGGCAACACGAGGATACAGACCTGGACAAGCTTTAATGCTGTGGAAACGTCTTTATGGGGATAACATTTGGGCTCATACCAGCGATGAATTAGAAGGTTTGAACAAAGATTTTAAGAAAATGTTGATTGAAAACGCTGAGTTCAGGGCGCTGTCTCTGAGAGAAATTCTCCCTTCATCTGATGGAACGAGGAAGTTCTTTGGGTTTGTGCTCATAAAGATTCGTCTGATTAATTTTAAAGCTCACAGGATATTACAGATTTTGTTCAATCTGGAAGATGGATTGGTAATAGAAACTGTTGTTATACCTTGCGATAGAGGCAGGACTACCGTTTGCGTTTCTAGTCAAGTGGGCTGTGCCATGAACTGTCAATTCTGCTACACTGGCAGGCAAGCTTCAATTCGGATGGGCCTGAAGAGACATCTGACTGCTGCTGAGATAGTAGAACAGGCAGTTTTTGTGAGGCGTTTGCTTACTAGTGAAGTGGGTTTAATTACTAATGTCGTGTTTATGGGAATGGGAGAGCCGCTTCACAACATTGACAATGTCATTAAAGCTGCAAATATAATGGTTCATGAACAAGGCCTTCATTTCAGTCCTCGCAAGGTCACTGTTTCAACCAGTGGACTTGTTCCCCAGCTGAAACGTTTCCTGAATGAATGTAACTGTGCTTTAGCTGTTAGTTTGAATGCAACTACTGATGAGGTCAGGAATTGGATCATGCCAATAAACCGGAAGTATAAGTTAGGCTTGCTTCTTCAGACTTTACGTGAGGAACTTCGCTGCAAACACAATTACAAGGTTCTTTTTGAATATGTGATGCTTGCTGGGGTTAATGACAGCATTGAAGATGCGAACAGGATCGTTGATCTTGTCCAGGGTATTCCATGCAAGATTAACCTTATTTCATTTAATCCTCATTGTGGATCCCAATTTAGACCTACCTGCAAGGAGAAGATGATTGAGTTTCGAAATGTTTTGGCTGAAGCCGGGTTGACTGTTTTCTTGCGACTAAGTAGAGGTGATGACCAGATGGCTGCCTGTGGTCAGTTAGGCAAACCTGGTACTGTTCAAGCTCCTTTACTCCGTGTACCAGATCAATTCCAAATGGCAATGAAATTGTGTCCCTAGTTCCTACGACAAGGTATCAAAAACGTCGACATCACTCCATCGATGTGGCATAAAATGGTGTGCCTAATCATGCAATGGTTAGAACTTATGCCCGTCTTCAATTTTTCAAGTCTTCTCGGAACGGAAGCTGTGGCCCTAGATCGAGAAATTCATCCCTCGTGTGTTCCTAAATTTATTTTCATTCTTCAGTTTTTCTTTTCAACTTGTACCCTTGTTTTTGGGATAAATTTTCGAATTGGTATTTAGAACTAGTTGTATTTCGGTGATGTGGACGTCGTGTGTTCGACTAACTTGAACTTGAC

Coding sequence (CDS)

ATGGTTTGGATTAGGCGCATGTTCTTCCCAAAGCCTTCCATGGCCTCCTCCATACTTGCCCCTTCGCACTTCCATTCCCTTTCTGTTCCTTCTGCACGCCTATCGCCACCATTGCACTTAATTTCTTTCCTCTCTACTGTTGGTCGCCCGTTTACGACTACCTCTTGCACATCACCTACGCCGTTATTTGCTCTTTACGATTCCTCACCTGATTTCTGTCTGTTCCTGACTATGCCTGAGGAAAAAGAAATCTTGAACAGTGATTCCGACTCCAAGATGCTTCTAAAGGGTCTGTCTTACACCGAACTTCAGGCAACACGAGGATACAGACCTGGACAAGCTTTAATGCTGTGGAAACGTCTTTATGGGGATAACATTTGGGCTCATACCAGCGATGAATTAGAAGGTTTGAACAAAGATTTTAAGAAAATGTTGATTGAAAACGCTGAGTTCAGGGCGCTGTCTCTGAGAGAAATTCTCCCTTCATCTGATGGAACGAGGAAGTTCTTTGGGTTTGTGCTCATAAAGATTCGTCTGATTAATTTTAAAGCTCACAGGATATTACAGATTTTGTTCAATCTGGAAGATGGATTGGTAATAGAAACTGTTGTTATACCTTGCGATAGAGGCAGGACTACCGTTTGCGTTTCTAGTCAAGTGGGCTGTGCCATGAACTGTCAATTCTGCTACACTGGCAGGCAAGCTTCAATTCGGATGGGCCTGAAGAGACATCTGACTGCTGCTGAGATAGTAGAACAGGCAGTTTTTGTGAGGCGTTTGCTTACTAGTGAAGTGGGTTTAATTACTAATGTCGTGTTTATGGGAATGGGAGAGCCGCTTCACAACATTGACAATGTCATTAAAGCTGCAAATATAATGGTTCATGAACAAGGCCTTCATTTCAGTCCTCGCAAGGTCACTGTTTCAACCAGTGGACTTGTTCCCCAGCTGAAACGTTTCCTGAATGAATGTAACTGTGCTTTAGCTGTTAGTTTGAATGCAACTACTGATGAGGTCAGGAATTGGATCATGCCAATAAACCGGAAGTATAAGTTAGGCTTGCTTCTTCAGACTTTACGTGAGGAACTTCGCTGCAAACACAATTACAAGGTTCTTTTTGAATATGTGATGCTTGCTGGGGTTAATGACAGCATTGAAGATGCGAACAGGATCGTTGATCTTGTCCAGGGTATTCCATGCAAGATTAACCTTATTTCATTTAATCCTCATTGTGGATCCCAATTTAGACCTACCTGCAAGGAGAAGATGATTGAGTTTCGAAATGTTTTGGCTGAAGCCGGGTTGACTGTTTTCTTGCGACTAAGTAGAGGTGATGACCAGATGGCTGCCTGTGGTCAGTTAGGCAAACCTGGTACTGTTCAAGCTCCTTTACTCCGTGTACCAGATCAATTCCAAATGGCAATGAAATTGTGTCCCTAG

Protein sequence

MVWIRRMFFPKPSMASSILAPSHFHSLSVPSARLSPPLHLISFLSTVGRPFTTTSCTSPTPLFALYDSSPDFCLFLTMPEEKEILNSDSDSKMLLKGLSYTELQATRGYRPGQALMLWKRLYGDNIWAHTSDELEGLNKDFKKMLIENAEFRALSLREILPSSDGTRKFFGFVLIKIRLINFKAHRILQILFNLEDGLVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIRMGLKRHLTAAEIVEQAVFVRRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMVHEQGLHFSPRKVTVSTSGLVPQLKRFLNECNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLREELRCKHNYKVLFEYVMLAGVNDSIEDANRIVDLVQGIPCKINLISFNPHCGSQFRPTCKEKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTVQAPLLRVPDQFQMAMKLCP
Homology
BLAST of ClCG08G018080 vs. NCBI nr
Match: XP_023546952.1 (uncharacterized protein LOC111805899 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 796.2 bits (2055), Expect = 1.6e-226
Identity = 410/479 (85.59%), Postives = 426/479 (88.94%), Query Frame = 0

Query: 4   IRRMFFPKPSMASSILAPSHFHSLSVPSARLSPPLHLISFLSTVGRPFTTTSCTSPTPLF 63
           +RR+FFPKPSMASSILAPSHF+SLS+PSARL PPLHLISFLS+ GR FTT S  SPTPLF
Sbjct: 1   MRRLFFPKPSMASSILAPSHFYSLSIPSARLCPPLHLISFLSSAGRRFTTASSASPTPLF 60

Query: 64  ALYDSSPDFCLFLTMPEEKEILNSDSDSKMLLKGLSYTELQ---ATRGYRPGQALMLWKR 123
           AL DSSPDF    +MPE+KEIL+  +DSKMLLKGLSYTEL+     RGYRPGQALMLWKR
Sbjct: 61  ALDDSSPDF----SMPEKKEILH--TDSKMLLKGLSYTELEKWVKARGYRPGQALMLWKR 120

Query: 124 LYGDNIWAHTSDELEGLNKDFKKMLIENAEFRALSLREILPSSDGTRKFFGFVLIKIRLI 183
           LYGDN+WAH+ DELEGLNKDFKKMLIE AEFRALSLREILPSSDGTRK            
Sbjct: 121 LYGDNVWAHSGDELEGLNKDFKKMLIEKAEFRALSLREILPSSDGTRK------------ 180

Query: 184 NFKAHRILQILFNLEDGLVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIRMG 243
                    ILFNLEDGL+IETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG     RMG
Sbjct: 181 ---------ILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG-----RMG 240

Query: 244 LKRHLTAAEIVEQAVFVRRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMVHEQGLH 303
           LKRHLTAAEIVEQAVF RRLLT EVGLITNVVFMGMGEPLHNIDNVIKAANIMV EQGLH
Sbjct: 241 LKRHLTAAEIVEQAVFARRLLTCEVGLITNVVFMGMGEPLHNIDNVIKAANIMVDEQGLH 300

Query: 304 FSPRKVTVSTSGLVPQLKRFLNECNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR 363
           FSPRKVTVSTSGLVPQLKRFL++CNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR
Sbjct: 301 FSPRKVTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR 360

Query: 364 EELRCKHNYKVLFEYVMLAGVNDSIEDANRIVDLVQGIPCKINLISFNPHCGSQFRPTCK 423
           EELRCKHNYKVLFEYVMLAGVNDSIEDA RIVDLVQGIPCKINLISFNPHCGSQFRPTCK
Sbjct: 361 EELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCGSQFRPTCK 420

Query: 424 EKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTVQAPLLRVPDQFQMAMKLCP 480
           EKMIEFRNVLA AGLTV LRLSRGDDQMAACGQLGKPGT+QAPLLRVPDQFQMAMKL P
Sbjct: 421 EKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQLGKPGTIQAPLLRVPDQFQMAMKLAP 447

BLAST of ClCG08G018080 vs. NCBI nr
Match: XP_022961770.1 (uncharacterized protein LOC111462441 isoform X1 [Cucurbita moschata])

HSP 1 Score: 795.0 bits (2052), Expect = 3.5e-226
Identity = 409/479 (85.39%), Postives = 426/479 (88.94%), Query Frame = 0

Query: 4   IRRMFFPKPSMASSILAPSHFHSLSVPSARLSPPLHLISFLSTVGRPFTTTSCTSPTPLF 63
           +RR+FFPKPSMASSILAPSHF+SLS+PSARL PPLHLISFLS+ GR FTT S  SPTPLF
Sbjct: 1   MRRLFFPKPSMASSILAPSHFYSLSIPSARLCPPLHLISFLSSAGRRFTTASSASPTPLF 60

Query: 64  ALYDSSPDFCLFLTMPEEKEILNSDSDSKMLLKGLSYTELQ---ATRGYRPGQALMLWKR 123
           AL DSSPDF    +MPE+KEIL+  +DSKMLLKGLSYTEL+     RGYRPGQALMLWKR
Sbjct: 61  ALDDSSPDF----SMPEKKEILH--TDSKMLLKGLSYTELEKWVKARGYRPGQALMLWKR 120

Query: 124 LYGDNIWAHTSDELEGLNKDFKKMLIENAEFRALSLREILPSSDGTRKFFGFVLIKIRLI 183
           LYGDN+WAH+ DELEGLNKDFKKMLIE AEFRALSLREILPSSDGTRK            
Sbjct: 121 LYGDNVWAHSGDELEGLNKDFKKMLIEKAEFRALSLREILPSSDGTRK------------ 180

Query: 184 NFKAHRILQILFNLEDGLVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIRMG 243
                    ILFNLEDGL+IETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG     RMG
Sbjct: 181 ---------ILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG-----RMG 240

Query: 244 LKRHLTAAEIVEQAVFVRRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMVHEQGLH 303
           LKRHLTAAEIVEQAVF RRLLT +VGLITNVVFMGMGEPLHNIDNVIKAANIMV EQGLH
Sbjct: 241 LKRHLTAAEIVEQAVFARRLLTCDVGLITNVVFMGMGEPLHNIDNVIKAANIMVDEQGLH 300

Query: 304 FSPRKVTVSTSGLVPQLKRFLNECNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR 363
           FSPRKVTVSTSGLVPQLKRFL++CNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR
Sbjct: 301 FSPRKVTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR 360

Query: 364 EELRCKHNYKVLFEYVMLAGVNDSIEDANRIVDLVQGIPCKINLISFNPHCGSQFRPTCK 423
           EELRCKHNYKVLFEYVMLAGVNDSIEDA RIVDLVQGIPCKINLISFNPHCGSQFRPTCK
Sbjct: 361 EELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCGSQFRPTCK 420

Query: 424 EKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTVQAPLLRVPDQFQMAMKLCP 480
           EKMIEFRNVLA AGLTV LRLSRGDDQMAACGQLGKPGT+QAPLLRVPDQFQMAMKL P
Sbjct: 421 EKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQLGKPGTIQAPLLRVPDQFQMAMKLAP 447

BLAST of ClCG08G018080 vs. NCBI nr
Match: XP_022996535.1 (uncharacterized protein LOC111491756 isoform X1 [Cucurbita maxima])

HSP 1 Score: 793.9 bits (2049), Expect = 7.8e-226
Identity = 409/479 (85.39%), Postives = 425/479 (88.73%), Query Frame = 0

Query: 4   IRRMFFPKPSMASSILAPSHFHSLSVPSARLSPPLHLISFLSTVGRPFTTTSCTSPTPLF 63
           +RR+FFPKPSMASSILAPSHF+SLS+PSARL PPLHLISFLS+ GR FTT S  SP PLF
Sbjct: 1   MRRLFFPKPSMASSILAPSHFYSLSIPSARLCPPLHLISFLSSAGRRFTTASSASPKPLF 60

Query: 64  ALYDSSPDFCLFLTMPEEKEILNSDSDSKMLLKGLSYTELQ---ATRGYRPGQALMLWKR 123
           AL DSSPDF    +MPE+KEIL+  +DSKMLLKGLSYTEL+     RGYRPGQALMLWKR
Sbjct: 61  ALDDSSPDF----SMPEKKEILH--TDSKMLLKGLSYTELEKWVKARGYRPGQALMLWKR 120

Query: 124 LYGDNIWAHTSDELEGLNKDFKKMLIENAEFRALSLREILPSSDGTRKFFGFVLIKIRLI 183
           LYGDN+WAH+ DELEGLNKDFKKMLIE AEFRALSLREILPSSDGTRK            
Sbjct: 121 LYGDNVWAHSGDELEGLNKDFKKMLIEKAEFRALSLREILPSSDGTRK------------ 180

Query: 184 NFKAHRILQILFNLEDGLVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIRMG 243
                    ILFNLEDGL+IETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG     RMG
Sbjct: 181 ---------ILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG-----RMG 240

Query: 244 LKRHLTAAEIVEQAVFVRRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMVHEQGLH 303
           LKRHLTAAEIVEQAVF RRLLT EVGLITNVVFMGMGEPLHNIDNVIKAANIMV EQGLH
Sbjct: 241 LKRHLTAAEIVEQAVFARRLLTCEVGLITNVVFMGMGEPLHNIDNVIKAANIMVDEQGLH 300

Query: 304 FSPRKVTVSTSGLVPQLKRFLNECNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR 363
           FSPRKVTVSTSGLVPQLKRFL++CNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR
Sbjct: 301 FSPRKVTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR 360

Query: 364 EELRCKHNYKVLFEYVMLAGVNDSIEDANRIVDLVQGIPCKINLISFNPHCGSQFRPTCK 423
           EELRCKHNYKVLFEYVMLAGVNDSIEDA RIVDLVQGIPCKINLISFNPHCGSQFRPTCK
Sbjct: 361 EELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCGSQFRPTCK 420

Query: 424 EKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTVQAPLLRVPDQFQMAMKLCP 480
           EKMIEFRNVLA AGLTV LRLSRGDDQMAACGQLGKPGT+QAPLLRVPDQFQMAMKL P
Sbjct: 421 EKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQLGKPGTIQAPLLRVPDQFQMAMKLAP 447

BLAST of ClCG08G018080 vs. NCBI nr
Match: KAG6598204.1 (hypothetical protein SDJN03_07982, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 793.9 bits (2049), Expect = 7.8e-226
Identity = 408/479 (85.18%), Postives = 426/479 (88.94%), Query Frame = 0

Query: 4   IRRMFFPKPSMASSILAPSHFHSLSVPSARLSPPLHLISFLSTVGRPFTTTSCTSPTPLF 63
           +RR+FFPKPSMASSILAPSHF+SLS+PSARL PPLHLI+FLS+ GR FTT S  SPTPLF
Sbjct: 1   MRRLFFPKPSMASSILAPSHFYSLSIPSARLCPPLHLITFLSSAGRRFTTASSASPTPLF 60

Query: 64  ALYDSSPDFCLFLTMPEEKEILNSDSDSKMLLKGLSYTELQ---ATRGYRPGQALMLWKR 123
           AL DSSPDF    +MPE+KEIL+  +DSKMLLKGLSYTEL+     RGYRPGQALMLWKR
Sbjct: 61  ALDDSSPDF----SMPEKKEILH--TDSKMLLKGLSYTELEKWVKARGYRPGQALMLWKR 120

Query: 124 LYGDNIWAHTSDELEGLNKDFKKMLIENAEFRALSLREILPSSDGTRKFFGFVLIKIRLI 183
           LYGDN+WAH+ DELEGLNKDFKKMLIE AEFRALSLREILPSSDGTRK            
Sbjct: 121 LYGDNVWAHSGDELEGLNKDFKKMLIEKAEFRALSLREILPSSDGTRK------------ 180

Query: 184 NFKAHRILQILFNLEDGLVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIRMG 243
                    ILFNLEDGL+IETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG     RMG
Sbjct: 181 ---------ILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG-----RMG 240

Query: 244 LKRHLTAAEIVEQAVFVRRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMVHEQGLH 303
           LKRHLTAAEIVEQAVF RRLLT +VGLITNVVFMGMGEPLHNIDNVIKAANIMV EQGLH
Sbjct: 241 LKRHLTAAEIVEQAVFARRLLTCDVGLITNVVFMGMGEPLHNIDNVIKAANIMVDEQGLH 300

Query: 304 FSPRKVTVSTSGLVPQLKRFLNECNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR 363
           FSPRKVTVSTSGLVPQLKRFL++CNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR
Sbjct: 301 FSPRKVTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR 360

Query: 364 EELRCKHNYKVLFEYVMLAGVNDSIEDANRIVDLVQGIPCKINLISFNPHCGSQFRPTCK 423
           EELRCKHNYKVLFEYVMLAGVNDSIEDA RIVDLVQGIPCKINLISFNPHCGSQFRPTCK
Sbjct: 361 EELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCGSQFRPTCK 420

Query: 424 EKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTVQAPLLRVPDQFQMAMKLCP 480
           EKMIEFRNVLA AGLTV LRLSRGDDQMAACGQLGKPGT+QAPLLRVPDQFQMAMKL P
Sbjct: 421 EKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQLGKPGTIQAPLLRVPDQFQMAMKLAP 447

BLAST of ClCG08G018080 vs. NCBI nr
Match: XP_022159137.1 (uncharacterized protein LOC111025565 isoform X1 [Momordica charantia])

HSP 1 Score: 747.3 bits (1928), Expect = 8.3e-212
Identity = 392/483 (81.16%), Postives = 414/483 (85.71%), Query Frame = 0

Query: 4   IRRMFFPKPSMASSIL--APSHFHSLSVPSARLSPPLHLISFLSTVGRPFTTTSCTSPTP 63
           +RR+FFPK SMASSIL  A SHF  LSVPS+RLS P HLISF+ +  RP +T SC SPTP
Sbjct: 1   MRRLFFPKASMASSILAVARSHFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP 60

Query: 64  LFALYDSSPDFC----LFLTMPEEKEILNSDSDSKMLLKGLSYTELQ---ATRGYRPGQA 123
           LFAL DSS DFC    +F  M +++EIL   +DSK+LLKGLSYTEL+    +RGYRPGQA
Sbjct: 61  LFALDDSSSDFCQFPNIFGFMSDKREILK--NDSKVLLKGLSYTELEKWVQSRGYRPGQA 120

Query: 124 LMLWKRLYGDNIWAHTSDELEGLNKDFKKMLIENAEFRALSLREILPSSDGTRKFFGFVL 183
           LMLWKRLYGDNIWAHT DELEGLNK FKKMLIENAEFRALSLREILPSSDGTRK      
Sbjct: 121 LMLWKRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRK------ 180

Query: 184 IKIRLINFKAHRILQILFNLEDGLVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQ 243
                          ILF+LEDGL+IETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG  
Sbjct: 181 ---------------ILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG-- 240

Query: 244 ASIRMGLKRHLTAAEIVEQAVFVRRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMV 303
              RMGLKRHLTAAEIVEQAVF RRLLTSEVG ITNVVFMGMGEPLHNIDNV KAANIMV
Sbjct: 241 ---RMGLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMV 300

Query: 304 HEQGLHFSPRKVTVSTSGLVPQLKRFLNECNCALAVSLNATTDEVRNWIMPINRKYKLGL 363
           HEQGLHFSPRKVTVSTSGLVPQLKRFL EC+CALAVSLNATTDEVRNWIMPINRKYKLGL
Sbjct: 301 HEQGLHFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGL 360

Query: 364 LLQTLREELRCKHNYKVLFEYVMLAGVNDSIEDANRIVDLVQGIPCKINLISFNPHCGSQ 423
           LLQTLREEL  KHNYKV FEYVMLAGVND +EDA R+VDLVQGIPCKINLISFNPH GSQ
Sbjct: 361 LLQTLREELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQ 420

Query: 424 FRPTCKEKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTVQAPLLRVPDQFQMA 478
           FRPT +EKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGT+QAPLLRVP+QFQMA
Sbjct: 421 FRPTRREKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMA 455

BLAST of ClCG08G018080 vs. ExPASy Swiss-Prot
Match: Q74E53 (Dual-specificity RNA methyltransferase RlmN OS=Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) OX=243231 GN=rlmN PE=3 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 7.4e-70
Identity = 162/368 (44.02%), Postives = 214/368 (58.15%), Query Frame = 0

Query: 90  DSKMLLKGLSYTELQ---ATRGYRPGQALMLWKRLYGDNIWAHTSDELEGLNKDFKKMLI 149
           D+ + +KGLS  EL+     +G    +A  ++K LY     A +  E+  L K+ ++ L 
Sbjct: 2   DTMIDIKGLSIDELERFLLGKGKERYRARQIFKWLYQRG--ATSFAEMTDLAKELRRDLE 61

Query: 150 ENAEFRALSLREILPSSDGTRKFFGFVLIKIRLINFKAHRILQILFNLEDGLVIETVVIP 209
           E A    LS   +  S DGTRK+                     LF L+DG  +E+V+IP
Sbjct: 62  ETARISTLSPEALEISRDGTRKY---------------------LFRLDDGCSVESVLIP 121

Query: 210 CDRGRTTVCVSSQVGCAMNCQFCYTGRQASIRMGLKRHLTAAEIVEQAVFVRRLLTSEVG 269
            +  R T+C+SSQVGCAM C+FC TG        L R+LT AEIV Q   V+R +     
Sbjct: 122 -EEDRNTLCISSQVGCAMACEFCLTG-----TFRLTRNLTTAEIVNQVCAVQRDVP---- 181

Query: 270 LITNVVFMGMGEPLHNIDNVIKAANIMVHEQGLHFSPRKVTVSTSGLVPQLKRFLNECNC 329
            + N+VFMGMGEPL N+DNVI+A  IM+H+ GL FS R++TVST+GLVP+++R       
Sbjct: 182 -VRNIVFMGMGEPLANLDNVIRALQIMLHDDGLQFSTRRITVSTAGLVPEMERLGRSVTV 241

Query: 330 ALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLREELRCKHNYKVLFEYVMLAGVNDSIE 389
            LAVSLNATTDE+R+ IMPINRKY L +LL   R         K+  EYV+L GVND+++
Sbjct: 242 NLAVSLNATTDELRDRIMPINRKYPLAVLLDACR-RFPLPGRRKITIEYVLLGGVNDTLD 301

Query: 390 DANRIVDLVQGIPCKINLISFNPHCGSQFRPTCKEKMIEFRNVLAEAGLTVFLRLSRGDD 449
           DA R+V L+  IP KINLI FN H G  FR   ++ +  F   L +   TV  R SRG D
Sbjct: 302 DAKRLVRLLSDIPSKINLIPFNEHEGCSFRSPSQDAIDRFHRYLLDKHFTVITRSSRGAD 334

Query: 450 QMAACGQL 455
             AACGQL
Sbjct: 362 ISAACGQL 334

BLAST of ClCG08G018080 vs. ExPASy Swiss-Prot
Match: Q39S71 (Dual-specificity RNA methyltransferase RlmN OS=Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15) OX=269799 GN=rlmN PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 4.8e-69
Identity = 164/368 (44.57%), Postives = 213/368 (57.88%), Query Frame = 0

Query: 90  DSKMLLKGLSYTEL---QATRGYRPGQALMLWKRLYGDNIWAHTSDELEGLNKDFKKMLI 149
           + K+ +K L+  +L    A +G    +A  ++K LY  +  A +  E+  L KD ++ L 
Sbjct: 3   NGKIDIKNLTLDDLIAFLAGKGKERYRARQIFKWLYQKD--ARSFAEMTDLAKDLRRDLE 62

Query: 150 ENAEFRALSLREILPSSDGTRKFFGFVLIKIRLINFKAHRILQILFNLEDGLVIETVVIP 209
           E A    L    +  S DGTRK+                     LF LEDG  +E+V+IP
Sbjct: 63  ETAVISDLEPEAMEVSRDGTRKY---------------------LFRLEDGNTVESVLIP 122

Query: 210 CDRGRTTVCVSSQVGCAMNCQFCYTGRQASIRMGLKRHLTAAEIVEQAVFVRRLLTSEVG 269
            +  RTT+C+SSQVGCAM C+FC TG        L R+LTA EIV Q   VRR +     
Sbjct: 123 -EEDRTTLCISSQVGCAMACEFCLTG-----TFRLTRNLTAGEIVNQICAVRRDVP---- 182

Query: 270 LITNVVFMGMGEPLHNIDNVIKAANIMVHEQGLHFSPRKVTVSTSGLVPQLKRFLNECNC 329
            + N+VFMGMGEPL N+DNV+KA  I++H+ GL FS R+VTVSTSGLVP+++R   E   
Sbjct: 183 -VRNIVFMGMGEPLANLDNVVKALKIILHDDGLQFSTRRVTVSTSGLVPEMERLGREVTV 242

Query: 330 ALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLREELRCKHNYKVLFEYVMLAGVNDSIE 389
            LAVSLNATTDEVR+ IMP+NR+Y L LLL   R         K+  EYVM+ G+NDS+E
Sbjct: 243 NLAVSLNATTDEVRDRIMPVNRRYPLRLLLDACR-SYPLPGRRKITIEYVMIKGLNDSLE 302

Query: 390 DANRIVDLVQGIPCKINLISFNPHCGSQFRPTCKEKMIEFRNVLAEAGLTVFLRLSRGDD 449
           DA R+V L+  I  KINLI FN H G  F+   +  +  F + L     TV  R SRG D
Sbjct: 303 DAKRLVKLLSDISSKINLIPFNEHDGCSFKSPDQGAIDVFHSYLLSKHFTVITRSSRGSD 335

Query: 450 QMAACGQL 455
             AACGQL
Sbjct: 363 ISAACGQL 335

BLAST of ClCG08G018080 vs. ExPASy Swiss-Prot
Match: Q3A2Z4 (Dual-specificity RNA methyltransferase RlmN OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1) OX=338963 GN=rlmN PE=3 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 2.4e-68
Identity = 157/387 (40.57%), Postives = 213/387 (55.04%), Query Frame = 0

Query: 85  LNSDSDSKMLLKGLSYTEL------QATRGYRPGQAL-MLWKRLYGDNIWAHTSDELEGL 144
           ++ D D+++ LK  +  EL           +R GQ +  ++ RL  D       D +  L
Sbjct: 3   VSMDQDTRIDLKNFTLEELTEFLAGMGKERFRAGQVMRWMYHRLVDD------FDAMSDL 62

Query: 145 NKDFKKMLIENAEFRALSLREILPSSDGTRKFFGFVLIKIRLINFKAHRILQILFNLEDG 204
           +K  +  L + A    L+      S DGTRK+                     LF LEDG
Sbjct: 63  SKVLRAELHQRARISRLTPEATEDSRDGTRKY---------------------LFRLEDG 122

Query: 205 LVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIRMGLKRHLTAAEIVEQAVFV 264
             IE+V IP D  R T+C+S+QVGCAM C FC+TG       GL R+LT  EIV Q    
Sbjct: 123 ETIESVRIPMDDNRATLCISTQVGCAMGCVFCHTG-----SFGLVRNLTPGEIVNQV--- 182

Query: 265 RRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMVHEQGLHFSPRKVTVSTSGLVPQL 324
                   G + N+V MGMGEPLHN+DNV+KA  I+   QGL +SPRKVT+ST+GLVPQ+
Sbjct: 183 --CAALADGPVNNIVLMGMGEPLHNLDNVVKALQILYMPQGLDYSPRKVTLSTAGLVPQM 242

Query: 325 KRFLNECNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLRE-ELRCKHNYKVLFEYV 384
           +         LAVSLNATTDEVRN +MP+N++Y L  L+   R+  L  K   ++ FEY+
Sbjct: 243 QELGKRVRVNLAVSLNATTDEVRNRLMPVNQRYPLQQLMAACRQYPLHAKK--RITFEYI 302

Query: 385 MLAGVNDSIEDANRIVDLVQGIPCKINLISFNPHCGSQFRPTCKEKMIEFRNVLAEAGLT 444
           ++  VNDS +DA R+V L+ GI  K+N+I FN H  S+FR   +E++  F+  L + G+ 
Sbjct: 303 LIRDVNDSDQDARRLVKLLHGIKAKVNIIPFNEHSASEFRAPTEERISRFQGYLLDHGMV 350

Query: 445 VFLRLSRGDDQMAACGQLGKPGTVQAP 464
              R S+G D  AACGQL    TV  P
Sbjct: 363 AIRRASKGQDISAACGQLKGKLTVSPP 350

BLAST of ClCG08G018080 vs. ExPASy Swiss-Prot
Match: A1AL40 (Dual-specificity RNA methyltransferase RlmN OS=Pelobacter propionicus (strain DSM 2379 / NBRC 103807 / OttBd1) OX=338966 GN=rlmN PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.4e-65
Identity = 158/353 (44.76%), Postives = 201/353 (56.94%), Query Frame = 0

Query: 113 QALMLWKRLYGDNIWAHTSDELEGLNKDFKKMLIENAEFRALSLREILPSSDGTRKFFGF 172
           +A  ++K +Y  +  A +  E+  ++KD +  L   A    L    +    DGTRK+   
Sbjct: 28  RATQVFKWIYQHD--ARSFQEMTNISKDLRAELEAKAYISNLEPEAVEVGGDGTRKY--- 87

Query: 173 VLIKIRLINFKAHRILQILFNLEDGLVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG 232
                             LF LEDG  +E+V+IP D GR T+C+SSQVGCAM C FC TG
Sbjct: 88  ------------------LFGLEDGNSVESVLIP-DEGRNTLCISSQVGCAMGCAFCLTG 147

Query: 233 RQASIRMGLKRHLTAAEIVEQAVFVRRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANI 292
                   L R+LT AEIV Q + VRR +      I N+V MGMGEPLHN+DNVI A +I
Sbjct: 148 -----TFRLTRNLTTAEIVNQIMAVRRDVE-----IRNIVMMGMGEPLHNLDNVIPAIHI 207

Query: 293 MVHEQGLHFSPRKVTVSTSGLVPQLKRFLNEC-NCALAVSLNATTDEVRNWIMPINRKYK 352
           M+   GL  S R+VTVST GL P+++R   E  N  LAVSLNATTDE+R+ IMPINR+Y 
Sbjct: 208 MIDGNGLQLSNRRVTVSTCGLAPEMERLGRELPNVNLAVSLNATTDELRDRIMPINRRYP 267

Query: 353 LGLLLQTLREELRCKHNYKVLFEYVMLAGVNDSIEDANRIVDLVQGIPCKINLISFNPHC 412
           L  LL   R E       KV FEYVML G+ND++EDA R++ L   IP K+NLI FN   
Sbjct: 268 LKELLSACR-EFPLPGRRKVTFEYVMLGGLNDTLEDAKRLLRLTSDIPNKVNLIPFNEFQ 327

Query: 413 GSQFRPTCKEKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQL-GKPGTVQAP 464
           G +FR   +  +  F   L +  +TV  R SRG D  AACGQL GK    + P
Sbjct: 328 GCEFRSPTRAAIDAFHKYLIDRHVTVITRDSRGSDISAACGQLKGKLDAARQP 345

BLAST of ClCG08G018080 vs. ExPASy Swiss-Prot
Match: A5GEC2 (Dual-specificity RNA methyltransferase RlmN OS=Geobacter uraniireducens (strain Rf4) OX=351605 GN=rlmN PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 7.1e-65
Identity = 154/366 (42.08%), Postives = 206/366 (56.28%), Query Frame = 0

Query: 92  KMLLKGLSYTELQ---ATRGYRPGQALMLWKRLYGDNIWAHTSDELEGLNKDFKKMLIEN 151
           K+ +K  +  EL+   A +G    +A  ++K LY  +  A    ++  L+KDF++ L + 
Sbjct: 3   KVDIKNFTLQELEAYIAGQGKERFRAKQIFKWLYQQD--AREFADMTNLSKDFRQELEKT 62

Query: 152 AEFRALSLREILPSSDGTRKFFGFVLIKIRLINFKAHRILQILFNLEDGLVIETVVIPCD 211
           A    L    +  S+DGT+K+                     LF L DG  +E+V+IP D
Sbjct: 63  AWISNLDAEAVEASADGTKKY---------------------LFRLADGNAVESVLIP-D 122

Query: 212 RGRTTVCVSSQVGCAMNCQFCYTGRQASIRMGLKRHLTAAEIVEQAVFVRRLLTSEVGLI 271
             RTT+C+SSQVGCAM C+FC TG        L R+LT AEIV Q   V+R        +
Sbjct: 123 EDRTTLCISSQVGCAMGCEFCLTG-----TFKLTRNLTTAEIVNQVCAVKRQEP-----V 182

Query: 272 TNVVFMGMGEPLHNIDNVIKAANIMVHEQGLHFSPRKVTVSTSGLVPQLKRFLNECNCAL 331
            N+VFMGMGEPL N+ NV+ A  I+    G  FS RKVTVSTSGLVP++          L
Sbjct: 183 RNIVFMGMGEPLANLKNVVGALKILTDPDGFQFSTRKVTVSTSGLVPEMAELGASVTVNL 242

Query: 332 AVSLNATTDEVRNWIMPINRKYKLGLLLQTLREELRCKHNYKVLFEYVMLAGVNDSIEDA 391
           AVSLNATTDEVR+ IMPINR+Y L  LL   +        + +  EYVM+ GVNDS++DA
Sbjct: 243 AVSLNATTDEVRDRIMPINRRYPLKELLAACKAFPLPSRRW-ITIEYVMIRGVNDSLDDA 302

Query: 392 NRIVDLVQGIPCKINLISFNPHCGSQFRPTCKEKMIEFRNVLAEAGLTVFLRLSRGDDQM 451
            R+V L+  IP K+NLI FN H G  F+   ++ + +F   L +  +TV  R SRG D  
Sbjct: 303 KRLVRLISNIPSKVNLIPFNEHDGCTFQAPTQDSIDKFHKFLLDKHVTVITRSSRGSDIS 333

Query: 452 AACGQL 455
           AACGQL
Sbjct: 363 AACGQL 333

BLAST of ClCG08G018080 vs. ExPASy TrEMBL
Match: A0A6J1HD79 (uncharacterized protein LOC111462441 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462441 PE=4 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 1.7e-226
Identity = 409/479 (85.39%), Postives = 426/479 (88.94%), Query Frame = 0

Query: 4   IRRMFFPKPSMASSILAPSHFHSLSVPSARLSPPLHLISFLSTVGRPFTTTSCTSPTPLF 63
           +RR+FFPKPSMASSILAPSHF+SLS+PSARL PPLHLISFLS+ GR FTT S  SPTPLF
Sbjct: 1   MRRLFFPKPSMASSILAPSHFYSLSIPSARLCPPLHLISFLSSAGRRFTTASSASPTPLF 60

Query: 64  ALYDSSPDFCLFLTMPEEKEILNSDSDSKMLLKGLSYTELQ---ATRGYRPGQALMLWKR 123
           AL DSSPDF    +MPE+KEIL+  +DSKMLLKGLSYTEL+     RGYRPGQALMLWKR
Sbjct: 61  ALDDSSPDF----SMPEKKEILH--TDSKMLLKGLSYTELEKWVKARGYRPGQALMLWKR 120

Query: 124 LYGDNIWAHTSDELEGLNKDFKKMLIENAEFRALSLREILPSSDGTRKFFGFVLIKIRLI 183
           LYGDN+WAH+ DELEGLNKDFKKMLIE AEFRALSLREILPSSDGTRK            
Sbjct: 121 LYGDNVWAHSGDELEGLNKDFKKMLIEKAEFRALSLREILPSSDGTRK------------ 180

Query: 184 NFKAHRILQILFNLEDGLVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIRMG 243
                    ILFNLEDGL+IETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG     RMG
Sbjct: 181 ---------ILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG-----RMG 240

Query: 244 LKRHLTAAEIVEQAVFVRRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMVHEQGLH 303
           LKRHLTAAEIVEQAVF RRLLT +VGLITNVVFMGMGEPLHNIDNVIKAANIMV EQGLH
Sbjct: 241 LKRHLTAAEIVEQAVFARRLLTCDVGLITNVVFMGMGEPLHNIDNVIKAANIMVDEQGLH 300

Query: 304 FSPRKVTVSTSGLVPQLKRFLNECNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR 363
           FSPRKVTVSTSGLVPQLKRFL++CNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR
Sbjct: 301 FSPRKVTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR 360

Query: 364 EELRCKHNYKVLFEYVMLAGVNDSIEDANRIVDLVQGIPCKINLISFNPHCGSQFRPTCK 423
           EELRCKHNYKVLFEYVMLAGVNDSIEDA RIVDLVQGIPCKINLISFNPHCGSQFRPTCK
Sbjct: 361 EELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCGSQFRPTCK 420

Query: 424 EKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTVQAPLLRVPDQFQMAMKLCP 480
           EKMIEFRNVLA AGLTV LRLSRGDDQMAACGQLGKPGT+QAPLLRVPDQFQMAMKL P
Sbjct: 421 EKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQLGKPGTIQAPLLRVPDQFQMAMKLAP 447

BLAST of ClCG08G018080 vs. ExPASy TrEMBL
Match: A0A6J1K720 (uncharacterized protein LOC111491756 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491756 PE=4 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.8e-226
Identity = 409/479 (85.39%), Postives = 425/479 (88.73%), Query Frame = 0

Query: 4   IRRMFFPKPSMASSILAPSHFHSLSVPSARLSPPLHLISFLSTVGRPFTTTSCTSPTPLF 63
           +RR+FFPKPSMASSILAPSHF+SLS+PSARL PPLHLISFLS+ GR FTT S  SP PLF
Sbjct: 1   MRRLFFPKPSMASSILAPSHFYSLSIPSARLCPPLHLISFLSSAGRRFTTASSASPKPLF 60

Query: 64  ALYDSSPDFCLFLTMPEEKEILNSDSDSKMLLKGLSYTELQ---ATRGYRPGQALMLWKR 123
           AL DSSPDF    +MPE+KEIL+  +DSKMLLKGLSYTEL+     RGYRPGQALMLWKR
Sbjct: 61  ALDDSSPDF----SMPEKKEILH--TDSKMLLKGLSYTELEKWVKARGYRPGQALMLWKR 120

Query: 124 LYGDNIWAHTSDELEGLNKDFKKMLIENAEFRALSLREILPSSDGTRKFFGFVLIKIRLI 183
           LYGDN+WAH+ DELEGLNKDFKKMLIE AEFRALSLREILPSSDGTRK            
Sbjct: 121 LYGDNVWAHSGDELEGLNKDFKKMLIEKAEFRALSLREILPSSDGTRK------------ 180

Query: 184 NFKAHRILQILFNLEDGLVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIRMG 243
                    ILFNLEDGL+IETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG     RMG
Sbjct: 181 ---------ILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG-----RMG 240

Query: 244 LKRHLTAAEIVEQAVFVRRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMVHEQGLH 303
           LKRHLTAAEIVEQAVF RRLLT EVGLITNVVFMGMGEPLHNIDNVIKAANIMV EQGLH
Sbjct: 241 LKRHLTAAEIVEQAVFARRLLTCEVGLITNVVFMGMGEPLHNIDNVIKAANIMVDEQGLH 300

Query: 304 FSPRKVTVSTSGLVPQLKRFLNECNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR 363
           FSPRKVTVSTSGLVPQLKRFL++CNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR
Sbjct: 301 FSPRKVTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLR 360

Query: 364 EELRCKHNYKVLFEYVMLAGVNDSIEDANRIVDLVQGIPCKINLISFNPHCGSQFRPTCK 423
           EELRCKHNYKVLFEYVMLAGVNDSIEDA RIVDLVQGIPCKINLISFNPHCGSQFRPTCK
Sbjct: 361 EELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCGSQFRPTCK 420

Query: 424 EKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTVQAPLLRVPDQFQMAMKLCP 480
           EKMIEFRNVLA AGLTV LRLSRGDDQMAACGQLGKPGT+QAPLLRVPDQFQMAMKL P
Sbjct: 421 EKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQLGKPGTIQAPLLRVPDQFQMAMKLAP 447

BLAST of ClCG08G018080 vs. ExPASy TrEMBL
Match: A0A6J1DYZ7 (uncharacterized protein LOC111025565 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111025565 PE=4 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 4.0e-212
Identity = 392/483 (81.16%), Postives = 414/483 (85.71%), Query Frame = 0

Query: 4   IRRMFFPKPSMASSIL--APSHFHSLSVPSARLSPPLHLISFLSTVGRPFTTTSCTSPTP 63
           +RR+FFPK SMASSIL  A SHF  LSVPS+RLS P HLISF+ +  RP +T SC SPTP
Sbjct: 1   MRRLFFPKASMASSILAVARSHFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP 60

Query: 64  LFALYDSSPDFC----LFLTMPEEKEILNSDSDSKMLLKGLSYTELQ---ATRGYRPGQA 123
           LFAL DSS DFC    +F  M +++EIL   +DSK+LLKGLSYTEL+    +RGYRPGQA
Sbjct: 61  LFALDDSSSDFCQFPNIFGFMSDKREILK--NDSKVLLKGLSYTELEKWVQSRGYRPGQA 120

Query: 124 LMLWKRLYGDNIWAHTSDELEGLNKDFKKMLIENAEFRALSLREILPSSDGTRKFFGFVL 183
           LMLWKRLYGDNIWAHT DELEGLNK FKKMLIENAEFRALSLREILPSSDGTRK      
Sbjct: 121 LMLWKRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRK------ 180

Query: 184 IKIRLINFKAHRILQILFNLEDGLVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQ 243
                          ILF+LEDGL+IETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG  
Sbjct: 181 ---------------ILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG-- 240

Query: 244 ASIRMGLKRHLTAAEIVEQAVFVRRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMV 303
              RMGLKRHLTAAEIVEQAVF RRLLTSEVG ITNVVFMGMGEPLHNIDNV KAANIMV
Sbjct: 241 ---RMGLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMV 300

Query: 304 HEQGLHFSPRKVTVSTSGLVPQLKRFLNECNCALAVSLNATTDEVRNWIMPINRKYKLGL 363
           HEQGLHFSPRKVTVSTSGLVPQLKRFL EC+CALAVSLNATTDEVRNWIMPINRKYKLGL
Sbjct: 301 HEQGLHFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGL 360

Query: 364 LLQTLREELRCKHNYKVLFEYVMLAGVNDSIEDANRIVDLVQGIPCKINLISFNPHCGSQ 423
           LLQTLREEL  KHNYKV FEYVMLAGVND +EDA R+VDLVQGIPCKINLISFNPH GSQ
Sbjct: 361 LLQTLREELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQ 420

Query: 424 FRPTCKEKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTVQAPLLRVPDQFQMA 478
           FRPT +EKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGT+QAPLLRVP+QFQMA
Sbjct: 421 FRPTRREKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMA 455

BLAST of ClCG08G018080 vs. ExPASy TrEMBL
Match: A0A6J1DXZ7 (uncharacterized protein LOC111025565 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025565 PE=4 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 1.3e-210
Identity = 390/479 (81.42%), Postives = 411/479 (85.80%), Query Frame = 0

Query: 4   IRRMFFPKPSMASSIL--APSHFHSLSVPSARLSPPLHLISFLSTVGRPFTTTSCTSPTP 63
           +RR+FFPK SMASSIL  A SHF  LSVPS+RLS P HLISF+ +  RP +T SC SPTP
Sbjct: 1   MRRLFFPKASMASSILAVARSHFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP 60

Query: 64  LFALYDSSPDFCLFLTMPEEKEILNSDSDSKMLLKGLSYTELQ---ATRGYRPGQALMLW 123
           LFAL DSS DF     M +++EIL   +DSK+LLKGLSYTEL+    +RGYRPGQALMLW
Sbjct: 61  LFALDDSSSDF----FMSDKREILK--NDSKVLLKGLSYTELEKWVQSRGYRPGQALMLW 120

Query: 124 KRLYGDNIWAHTSDELEGLNKDFKKMLIENAEFRALSLREILPSSDGTRKFFGFVLIKIR 183
           KRLYGDNIWAHT DELEGLNK FKKMLIENAEFRALSLREILPSSDGTRK          
Sbjct: 121 KRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRK---------- 180

Query: 184 LINFKAHRILQILFNLEDGLVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIR 243
                      ILF+LEDGL+IETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG     R
Sbjct: 181 -----------ILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG-----R 240

Query: 244 MGLKRHLTAAEIVEQAVFVRRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMVHEQG 303
           MGLKRHLTAAEIVEQAVF RRLLTSEVG ITNVVFMGMGEPLHNIDNV KAANIMVHEQG
Sbjct: 241 MGLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQG 300

Query: 304 LHFSPRKVTVSTSGLVPQLKRFLNECNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQT 363
           LHFSPRKVTVSTSGLVPQLKRFL EC+CALAVSLNATTDEVRNWIMPINRKYKLGLLLQT
Sbjct: 301 LHFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQT 360

Query: 364 LREELRCKHNYKVLFEYVMLAGVNDSIEDANRIVDLVQGIPCKINLISFNPHCGSQFRPT 423
           LREEL  KHNYKV FEYVMLAGVND +EDA R+VDLVQGIPCKINLISFNPH GSQFRPT
Sbjct: 361 LREELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPT 420

Query: 424 CKEKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTVQAPLLRVPDQFQMAMKL 478
            +EKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGT+QAPLLRVP+QFQMAMKL
Sbjct: 421 RREKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKL 447

BLAST of ClCG08G018080 vs. ExPASy TrEMBL
Match: A0A1S3BV96 (dual-specificity RNA methyltransferase RlmN isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493873 PE=4 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 7.1e-201
Identity = 363/423 (85.82%), Postives = 375/423 (88.65%), Query Frame = 0

Query: 60  TPLFALYDSSPDFCLFLTMPEEKEILNSDSDSKMLLKGLSYTELQ---ATRGYRPGQALM 119
           T L + +++S   CL   MPEEKEILNSD +SKMLLKGLSYTEL+     +GYRPGQALM
Sbjct: 17  TSLLSFHNNSRR-CLSKAMPEEKEILNSDFNSKMLLKGLSYTELEKWVQAQGYRPGQALM 76

Query: 120 LWKRLYGDNIWAHTSDELEGLNKDFKKMLIENAEFRALSLREILPSSDGTRKFFGFVLIK 179
           LWKRLYGDNIWAHT DELEGLNKDFKKMLIENA+FRALSLREILPS DGTRK        
Sbjct: 77  LWKRLYGDNIWAHTGDELEGLNKDFKKMLIENAKFRALSLREILPSCDGTRK-------- 136

Query: 180 IRLINFKAHRILQILFNLEDGLVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQAS 239
                        ILFNLEDGL+IETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG    
Sbjct: 137 -------------ILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG---- 196

Query: 240 IRMGLKRHLTAAEIVEQAVFVRRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMVHE 299
            RMGLKRHLTAAEIVEQAVF RRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMVHE
Sbjct: 197 -RMGLKRHLTAAEIVEQAVFARRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMVHE 256

Query: 300 QGLHFSPRKVTVSTSGLVPQLKRFLNECNCALAVSLNATTDEVRNWIMPINRKYKLGLLL 359
           QGLHFSPRKVTVSTSGLVPQLKRFLNECNCALAVSLNATTDEVRNWIMPINRKYKLGLLL
Sbjct: 257 QGLHFSPRKVTVSTSGLVPQLKRFLNECNCALAVSLNATTDEVRNWIMPINRKYKLGLLL 316

Query: 360 QTLREELRCKHNYKVLFEYVMLAGVNDSIEDANRIVDLVQGIPCKINLISFNPHCGSQFR 419
           QTLREELRCKH YKVLFEYVMLAGVNDSIEDA RIVDLVQGIPCKINLISFNPHCGSQFR
Sbjct: 317 QTLREELRCKHKYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCGSQFR 376

Query: 420 PTCKEKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTVQAPLLRVPDQFQMAMK 479
           PTCKEKMI FRNVLAE+GLTVFLRLSRGDDQMAACGQLGKPG VQAPLLRVPD+FQMAM+
Sbjct: 377 PTCKEKMIGFRNVLAESGLTVFLRLSRGDDQMAACGQLGKPGIVQAPLLRVPDRFQMAME 412

BLAST of ClCG08G018080 vs. TAIR 10
Match: AT1G60230.1 (Radical SAM superfamily protein )

HSP 1 Score: 559.7 bits (1441), Expect = 2.3e-159
Identity = 302/477 (63.31%), Postives = 352/477 (73.79%), Query Frame = 0

Query: 4   IRRMFFPKPSMASSILAPSHFHSLSVPSARLSPPLHLISFLSTVGRPFTTTSCTSPTPLF 63
           +RR+     + +++++ PS   S +     L+ P  L+S   T    F  +   S +   
Sbjct: 5   MRRLGIITLTNSAAVVTPSLLSSTAAFPGTLTLPSPLLSDRLTNSILFHESRSLSFSCFS 64

Query: 64  ALYDSSPDFCLFL-TMPEEKEILNSDSDSKMLLKGLSYTELQ---ATRGYRPGQALMLWK 123
           +   SS  +   L   P  K  +  D + K++LKG++Y  LQ    + G+RPGQALMLWK
Sbjct: 65  SSSSSSAAYLPSLEEYPSAKGSVKRDDNQKVVLKGMTYASLQEWVQSHGFRPGQALMLWK 124

Query: 124 RLYGDNIWAHTSDELEGLNKDFKKMLIENAEFRALSLREILPSSDGTRKFFGFVLIKIRL 183
           RLY DNIWA+  DELEGLNKD K+M+ E+AEF ALS ++I  +SDGTRK           
Sbjct: 125 RLYKDNIWANNVDELEGLNKDLKRMISEHAEFGALSFKDIRSASDGTRK----------- 184

Query: 184 INFKAHRILQILFNLEDGLVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIRM 243
                     ILF L+DGLVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG     RM
Sbjct: 185 ----------ILFTLDDGLVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG-----RM 244

Query: 244 GLKRHLTAAEIVEQAVFVRRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMVHEQGL 303
           GLKR+LT AEIVEQAV+ RRLL+ EVG ITNVVFMGMGEP HNIDNVIKAANIMV E GL
Sbjct: 245 GLKRNLTTAEIVEQAVYARRLLSHEVGSITNVVFMGMGEPFHNIDNVIKAANIMVDENGL 304

Query: 304 HFSPRKVTVSTSGLVPQLKRFLNECNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL 363
           HFSPRKVTVSTSGLVPQLKRFL E NCALAVSLNATTDEVRNWIMPINRKYKL LLL+TL
Sbjct: 305 HFSPRKVTVSTSGLVPQLKRFLRESNCALAVSLNATTDEVRNWIMPINRKYKLSLLLETL 364

Query: 364 REELRCKHNYKVLFEYVMLAGVNDSIEDANRIVDLVQGIPCKINLISFNPHCGSQFRPTC 423
           RE L  +H YKVLFEYVMLAGVNDS++DA R+V+LVQGIPCKINLI FNPH GSQF  T 
Sbjct: 365 REGLSSRHKYKVLFEYVMLAGVNDSMDDARRLVELVQGIPCKINLIQFNPHSGSQFIQTE 424

Query: 424 KEKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTVQAPLLRVPDQFQMAMK 477
           ++KMI+FRNVLAE G TV +R SRG+DQMAACGQLG  G VQAP++RVP+QF+ A+K
Sbjct: 425 EDKMIKFRNVLAEGGCTVLMRFSRGNDQMAACGQLGMIGAVQAPVMRVPEQFRTALK 455

BLAST of ClCG08G018080 vs. TAIR 10
Match: AT3G19630.1 (Radical SAM superfamily protein )

HSP 1 Score: 176.4 bits (446), Expect = 5.5e-44
Identity = 127/380 (33.42%), Postives = 186/380 (48.95%), Query Frame = 0

Query: 108 GYRPGQALMLWKRLY--GDNIWAHTSDELEGLNKDFKKMLIENAEFRALSLREILPSSDG 167
           G  P  A+ +WK +    D +W    DE+  L      +L    +    SL  +  SSDG
Sbjct: 20  GINPKFAIQIWKYVIQNPDCVW----DEIPSLPSAAYSLLHSKFKTLTSSLHSLFHSSDG 79

Query: 168 TRKFFGFVLIKIRLINFKAHRILQILFNLEDGLVIETVVIPCDRG-------------RT 227
           T                      ++L  L++G  +E VV+  D               R+
Sbjct: 80  TTS--------------------KLLIKLQNGAFVEAVVMRYDTRLGMLGGKPRPGGIRS 139

Query: 228 TVCVSSQVGCAMNCQFCYTGRQASIRMGLKRHLTAAEIVEQAVFVRRLLTSEVGLITNVV 287
           T+C+SSQVGC M C FC TG      MG K +LT+ EIVEQ V   R+       I N+V
Sbjct: 140 TLCISSQVGCKMGCTFCATG-----TMGFKSNLTSGEIVEQLVHASRIAD-----IRNIV 199

Query: 288 FMGMGEPLHNIDNVIKAANIMVHEQGLHFSPRKVTVSTSGLVPQLKRFLNEC-NCALAVS 347
           FMGMGEPL+N + V++A  +M++ Q    SP+++T+ST G+V  + +  N+    +LAVS
Sbjct: 200 FMGMGEPLNNYNAVVEAVRVMLN-QPFQLSPKRITISTVGIVHAINKLHNDLPGVSLAVS 259

Query: 348 LNATTDEVRNWIMPINRKYKLGLLLQTLREELRCKHNYKVLFEYVMLAGVNDSIEDANRI 407
           L+A   E+R  IMP  R + L  L+  L +  +     K+  EY+ML GVND  + A+ +
Sbjct: 260 LHAPVQEIRCQIMPAARAFPLQKLMDAL-QTFQKNSQQKIFIEYIMLDGVNDQEQHAHLL 319

Query: 408 VDLVQGIPCKINLISFNP-HCGSQFRPTCKEKMIEFRNVLAEA-GLTVFLRLSRGDDQMA 462
            +L++     INLI FNP    SQF  +  + +  F+ +L E   +   +R   G D   
Sbjct: 320 GELLKTFQVVINLIPFNPIGSTSQFETSSIQGVSRFQKILRETYKIRTTIRKEMGQDISG 363

BLAST of ClCG08G018080 vs. TAIR 10
Match: AT2G39670.1 (Radical SAM superfamily protein )

HSP 1 Score: 167.2 bits (422), Expect = 3.3e-41
Identity = 107/290 (36.90%), Postives = 165/290 (56.90%), Query Frame = 0

Query: 188 LQILFNLEDGLVIETVVIPC---DRG--RTTVCVSSQVGCAMNCQFCYTGRQASIRMGLK 247
           +++L  LED L+IETV IP    ++G  R T CVSSQVGC + C FC TG+      G  
Sbjct: 138 IKLLLKLEDNLLIETVGIPVQDDEKGITRLTACVSSQVGCPLRCSFCATGKG-----GFS 197

Query: 248 RHLTAAEIVEQAVFVRRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMVHEQGLHFS 307
           R+L   EI+EQ + +  +    V   TNVVFMGMGEP+ N+ +V+ A   +   + +   
Sbjct: 198 RNLQRHEIIEQVLAIEDVFKHRV---TNVVFMGMGEPMLNLKSVLDAHRCL--NKDIEIG 257

Query: 308 PRKVTVSTSGLVPQLKRFL-NECNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLRE 367
            R +T+ST G+   +K+   ++    LAVSL+A    +R  I+P  + Y L  +++  R+
Sbjct: 258 QRMITISTVGVPNTIKKLASHKLQSTLAVSLHAPNQSLREKIVPSAKAYPLEAIMKDCRD 317

Query: 368 ELRCKHNYKVLFEYVMLAGVNDSIEDANRIVDLVQ--GIPCKINLISFNPHCGSQFRPTC 427
             + + N +V FEY +LAGVND +E A  + +L++  G    +NLI +NP  GS+++   
Sbjct: 318 YFQ-ETNRRVSFEYALLAGVNDQVEHAVELAELLREWGKTYHVNLIPYNPIEGSEYQRPY 377

Query: 428 KEKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTVQAPLLRVPD 470
           K+ ++ F   L    +T  +R +RG D  AACGQL +    ++PLL   D
Sbjct: 378 KKAVLAFAAALESRKITASVRQTRGLDASAACGQL-RNKFQKSPLLTETD 415

BLAST of ClCG08G018080 vs. TAIR 10
Match: AT2G39670.2 (Radical SAM superfamily protein )

HSP 1 Score: 167.2 bits (422), Expect = 3.3e-41
Identity = 107/290 (36.90%), Postives = 165/290 (56.90%), Query Frame = 0

Query: 188 LQILFNLEDGLVIETVVIPC---DRG--RTTVCVSSQVGCAMNCQFCYTGRQASIRMGLK 247
           +++L  LED L+IETV IP    ++G  R T CVSSQVGC + C FC TG+      G  
Sbjct: 141 IKLLLKLEDNLLIETVGIPVQDDEKGITRLTACVSSQVGCPLRCSFCATGKG-----GFS 200

Query: 248 RHLTAAEIVEQAVFVRRLLTSEVGLITNVVFMGMGEPLHNIDNVIKAANIMVHEQGLHFS 307
           R+L   EI+EQ + +  +    V   TNVVFMGMGEP+ N+ +V+ A   +   + +   
Sbjct: 201 RNLQRHEIIEQVLAIEDVFKHRV---TNVVFMGMGEPMLNLKSVLDAHRCL--NKDIEIG 260

Query: 308 PRKVTVSTSGLVPQLKRFL-NECNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLRE 367
            R +T+ST G+   +K+   ++    LAVSL+A    +R  I+P  + Y L  +++  R+
Sbjct: 261 QRMITISTVGVPNTIKKLASHKLQSTLAVSLHAPNQSLREKIVPSAKAYPLEAIMKDCRD 320

Query: 368 ELRCKHNYKVLFEYVMLAGVNDSIEDANRIVDLVQ--GIPCKINLISFNPHCGSQFRPTC 427
             + + N +V FEY +LAGVND +E A  + +L++  G    +NLI +NP  GS+++   
Sbjct: 321 YFQ-ETNRRVSFEYALLAGVNDQVEHAVELAELLREWGKTYHVNLIPYNPIEGSEYQRPY 380

Query: 428 KEKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTVQAPLLRVPD 470
           K+ ++ F   L    +T  +R +RG D  AACGQL +    ++PLL   D
Sbjct: 381 KKAVLAFAAALESRKITASVRQTRGLDASAACGQL-RNKFQKSPLLTETD 418

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023546952.11.6e-22685.59uncharacterized protein LOC111805899 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022961770.13.5e-22685.39uncharacterized protein LOC111462441 isoform X1 [Cucurbita moschata][more]
XP_022996535.17.8e-22685.39uncharacterized protein LOC111491756 isoform X1 [Cucurbita maxima][more]
KAG6598204.17.8e-22685.18hypothetical protein SDJN03_07982, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022159137.18.3e-21281.16uncharacterized protein LOC111025565 isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q74E537.4e-7044.02Dual-specificity RNA methyltransferase RlmN OS=Geobacter sulfurreducens (strain ... [more]
Q39S714.8e-6944.57Dual-specificity RNA methyltransferase RlmN OS=Geobacter metallireducens (strain... [more]
Q3A2Z42.4e-6840.57Dual-specificity RNA methyltransferase RlmN OS=Pelobacter carbinolicus (strain D... [more]
A1AL401.4e-6544.76Dual-specificity RNA methyltransferase RlmN OS=Pelobacter propionicus (strain DS... [more]
A5GEC27.1e-6542.08Dual-specificity RNA methyltransferase RlmN OS=Geobacter uraniireducens (strain ... [more]
Match NameE-valueIdentityDescription
A0A6J1HD791.7e-22685.39uncharacterized protein LOC111462441 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1K7203.8e-22685.39uncharacterized protein LOC111491756 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1DYZ74.0e-21281.16uncharacterized protein LOC111025565 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1DXZ71.3e-21081.42uncharacterized protein LOC111025565 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A1S3BV967.1e-20185.82dual-specificity RNA methyltransferase RlmN isoform X2 OS=Cucumis melo OX=3656 G... [more]
Match NameE-valueIdentityDescription
AT1G60230.12.3e-15963.31Radical SAM superfamily protein [more]
AT3G19630.15.5e-4433.42Radical SAM superfamily protein [more]
AT2G39670.13.3e-4136.90Radical SAM superfamily protein [more]
AT2G39670.23.3e-4136.90Radical SAM superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027492Dual-specificity RNA methyltransferase RlmNTIGRFAMTIGR00048TIGR00048coord: 106..455
e-value: 1.8E-88
score: 294.8
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 176..460
e-value: 6.6E-103
score: 345.7
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 217..384
e-value: 4.5E-17
score: 62.9
IPR007197Radical SAMPROSITEPS51918RADICAL_SAMcoord: 208..446
score: 29.8375
NoneNo IPR availablePFAMPF13394Fer4_14coord: 221..327
e-value: 1.1E-6
score: 28.7
NoneNo IPR availableSFLDSFLDG01062methyltransferase_(Class_A)coord: 92..456
e-value: 0.0
score: 342.2
NoneNo IPR availablePANTHERPTHR30544:SF5RADICAL SAM SUPERFAMILY PROTEINcoord: 73..462
NoneNo IPR availableCDDcd01335Radical_SAMcoord: 221..388
e-value: 1.73251E-6
score: 46.5578
NoneNo IPR availableSUPERFAMILY102114Radical SAM enzymescoord: 210..435
IPR004383Ribosomal RNA large subunit methyltransferase RlmN/CfrSFLDSFLDF00275adenosine_C2_methyltransferascoord: 92..456
e-value: 0.0
score: 342.2
IPR040072Methyltransferase (Class A)PANTHERPTHR3054423S RRNA METHYLTRANSFERASEcoord: 73..462

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG08G018080.1ClCG08G018080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070475 rRNA base methylation
biological_process GO:0030488 tRNA methylation
biological_process GO:0006364 rRNA processing
cellular_component GO:0005737 cytoplasm
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008173 RNA methyltransferase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding