ClCG04G004060 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG04G004060
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionL-galactono-1,4-lactone dehydrogenase, mitochondrial
LocationCG_Chr04: 15617343 .. 15622557 (-)
RNA-Seq ExpressionClCG04G004060
SyntenyClCG04G004060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTGTAAGCTGTAACTCATCTCCGAACTTCGAATTTTCACTTCAAACAGCTCAATTGAATTCCTCTATTGGGCGACCCATAAACCCAAGAATCGATCTGAAGCCACTCCACCACACTATCAAATGCTCAAACTTCTCGCGCTCCGCCGATCCTTCCATTGCTTCCGCCTCCGCCGTCTCCAAATTCCACCCACCACCGCCATCTCCAAACCCCCTTTGAATCCCCCTCGCCCCTTTTCCACTGTTTCTTCTCCACCATCCTCCTCCTCCTTCGATTCCGAGCTTCGTAAGTACCTCGGCTATGGCGCTCTCGTCATTTTCTGCGGCGCCGCCACTTACTACTCCTTCCCTTTCCCCGAAAACGCCAAGCACAAGAAGGCCCAGATCTTTCGATACGCTCCTCTGCCTGAGGATCTTCACACTGTATCCAATTGGAGTGGAACTCATGAGGTTCTAACGAGAGTGTTTCACCAGCCGGAGAACTTGGAGCAACTTGAGCAAGTGGTAAAGGAGGCCAATGAAAAGAAGGCTAGGATTAGGCCTGTTGGCTCGGGGCTTTCGCCTAATGGGATTGGACTTTCGAAAATGGGGATGGTGAATTTGGCGTTAATGGATAAGGTTTTGGAAGTAGATAAGGAGAAGAAGAGGGTTAGGGTTCAAGCGGGAATAAGGGTTCAGCAGCTTGTAGATGGAATCAAGGAGTATGGGCTTACTTTGCAGAATTTTGCTTCTATCAGGGAGCAGCAAATTGGTGGCATCATTCAGGTGGTTTTATTAGGATTTTTGTGTTTTTTCATGTTGTTCAATTGCTAATTGAATTGCAATGTTGGATTGTCTGCTGCATTTTGATCTATCTGAAAACTTGACTTTCAATGCCATGGCTGTGCGTTGATAAATATGAACAATTGTCTCTCCAGGGGTGTACATTAGTCTGTTTTGAGTGAAGACAAAGGATACCATTGATTTCTTGTCTTAAGTATATTGAAACTAATTAAATTTGATATGTAAACTTCCTATCGATCTTGATTAGTTTGTATACTGTATTTCTTCTTGTGACCGAAATATGATTTTAAAGGCTGGGCGAGCTTAGTAGTAGTGCTAGTACTGGTCAAGTGGTTACATTTGAAACAAAATTTATCTCGTTTGAAAGAGAAACGATCAAGTTGTTACTACTCTGGATACGAAGTGAACATTGATTGGGTAGATTGGTTATTTCTTTCATATTTTACTTTGTTTGATTGGAGTCCCTTTTTGTTTTAGTTTGGCTTATTTTGGTTGGGGCTTTATTTTCTAGGTCCCTCATATTCTTTCTTTTGTCCTTTATAAAAAGAACGAAAGACTATTTTAGGTGGCATAACTACTATATGTGGGACTCAATGGAAGTTTTCAATTTTTATTTTTACTGGAATCTCATCTTTTCTTTTTGCTTCTACAATGAGATGTTTCCAAAGTGTGCAATGTCAGATTTTTGTTTAGTCATGTATTTTCTTGAGCTGAGCCAAAGCTTTCTTTCTTGCTTATGCACACTTTTACGATTATGCATCTAGGTCGGTGCACATGGTTCCGGTGCAAAATTGCCACCCATTGATGAGCAGGTTATTGCTATGAAATTGGTCACTCCTGCCAAGGGTACTATTGAGATTTCCAAAGATAAAGATCCAGAGCTTTTTTTTCTGGCTCGCTGTGGTCTTGGGGGGCTTGGAGTGGTTGCTGAAGTAACCCTCCAGTGTGTCGAGAGAGAGGAACTTGTGGAGCACACATACATTTCGAACATGAAAGACATCAAGAAGAACCACAAGTAATTCTGTACTTTTTGATAGTTAATTTTTTGCAGTTCTATTGTATGTTGTAGACTACAGTTTGAAATGATAGTTTATATCTCACCTTATTTATTCTTATTGTTATTCTTATTTGAGAATGTTTAAAGGTTAATTGAAATATTTTAGGTCCCAGTATTCTGATAATCTAGAATTTAAAGACTAAATTTGAGTTATTTTGCTATCAAAGCATCTATACTCATATGTAGGGCCCCAAAGAGGTATTTGGTCCCATCTTTGATGCTTTTATCAAACTAGCATCCCATATCCTAGTGAAGGAATTACTAATTTTGAAGTTGAATTTTGCCATTTCTCAACCAAAAAGAGGTTGAATTACTATTATTTATTTATTTTTTAAAAAAATAGAAATAACTGCAATTGGCCTGCTTTCCTTACTCTTTGTAATTACTTTCTCTTTATGATTCTTGTTAGTTAAGATTTTTGTTTTTCTTTATATTTGTTATGTTTTTTTTGTTTCTTTTTGTATCTCAATTCATTGGTCTCTTTTCATTTACTTAATGTGAAGTTTTGTATCATTTTCTTACAAAAAAAAAAGAAAATTGCTTTTATTGAGAGAAAATGAAATAACACAAGGATGTACAAAAAACCAAGCCCACAAAATCTCCACTAGAGAAAGAGTTTTCAACTAAGTAAGATATTACCTAGAGAATAAATTTAAAAGGTTTTTAAGAAAGAAGCCCAAAATGAAACAAACCTCACCAAGGACTAAAGCTCTAGAGCTTGCCTGCCATCATCTTTTATTGTTTTATAGTATGCAAGGGTAATAGTATATAGATTGAAATCTAAACTATTGATTCATGGTTGGGGCGTGATGAGGGTGCTAGGCGGGTGTCAACCTAGTTGAGATGTCCCGGTGCACCTCCTGATCCCTCTTGCCTTGTCAATATTTTTTTAAAAAAACTATTGATTTATGGGAGTAGTTGCATAATTTAGATTGACGCTATACACATATAAGGTCTGACATTCCCTCTATGCAGGAAATTATTGGGTGACAATAAGCATGTGAAATACCTGTATATCCCTTACACCGATGCTATTGTTGTTGTGACTTGCAATCCTATCTCAAAATGGAGAGGCCCTCCCAAATTCAAACCGAAATATACTAGTGAGGAAGCCATACAACATGTTCGTGATCTCTACTTGGAGTCCCTTAAAAAATACAGGTTACTTCCATCTTCTGTTTTGAGTACTTAGTCATCAATAATTTTAGGATGTTGCAAATGGGTTGAAATGGAGATAGTTGACATTTTTATCTTCTCAAATCAATGTACATTCCTATTGTTTTGTTTCTCCTTCTCTTTTGGTTGATATTCATTTTTCATCAAAGAGTCATTTGTGGGCCTTTTTTCAAATTCTTTTTGCTGTATATATTTATAGATATATATTTTTCTTTCCCATTGTCTTCCAATTGCTTTTTATTTAATTGCTTGCTTTATAATTGCAGTGCTAGTGATGAAAGAGACATAAATGAATTTTCATTTACTGAGTTGCGTGATAAACTACTTGCCTTTGATCCTCTTAACAAAGAGCATGTAATGAAAGTGAATCAAGCAGAGGCTGAGTTCTGGAGGAAGTCGGAAGGATACAGAGTCGGATGGAGTGATGAAATTTTAGGTTTTGATTGTGGTGGCCAACAATGGGTCTCTGAAACTTGCTTTCCCGCCGGTACTCTTGCAAAACCTAACATGAAAGACATTGAATACATAGAGGAATTGAAGCAGCTTATCGAAAAAGAAAATATACCTGCACCTGCTCCTCTTGAGCAGCGGTGGACTGCTCGTAGTAAGAGCCCCATGAGTCCAGCTTCAAGCTCAGCTGAGGATGATATATTCTCTTGGGTAAACTCTAAACATCAAACTTTATATTTTCTCGTTCAAGTTTTAAATATATACTTTTGCTGCCCTGAGGGTTGCCCCAGTGAGATAAAGACGGGACATTTAGAGGTATCTTCCAGGATCTGGGAACTTAATAACTTAAAACAATCCTTGTCTTTTTGGAGTTGGTATTGGAGTAGGCGAGCTCCATAACCCTGAGTATAGTGGAAAGAGTGTGCTCCCACGTCTCGGTTAAAATTCGACATTCCACACTTGTGCTATTGTGCTTGAAGGGAGAAGCACAAGTTACCTTAAAAAAAATAGTGAAATTAGAAATCATGTATCATAAATTTTCTTTTCTGCTAAATGGGTCTTATATTCTGAAGAGTGTTGTGATCTTATATGATGATTAGTTGCTGACAAGTGACAACTCAAAATTATTTCATCTCAGAAATCCTAAGCAACACGGACATAGTTTGGCTAGCGTGTCCTTGTTGTGTCATACACTTAAACACTTCGACACTTGCTTAGACACGTATGGAACACTTGTTAGTGTAGATGTGTTAAATACTAGTTTTACAAAGTCAAAATAGGTATGATATATGTTAAACATGCATAAAATGTTTGCTTCGTGTACTAAATAGACACATAAATGACAAAATATATATATAAATGAAAAAAGTAAAAAACATAAAATTTAAGAGCAAAATACATAAGCTAAACTTTTAAGCATACAAATGCGAACTTGTTGACTTTGATGTTCCTTTTTGTTTAAAAATGATATATATTTAAGAAAACATATGTTTTAACCTTTAATACACGTGTCTTTGTTATGTCCGTGTCGTATCCGTATCTGTACTTCTTAGCATAAATCTCTACCCCATTGCCAAGTGATTTTATTTTGAAGTCTCGCCAATTAGAACTCGGTAGACCTGATCGTGGTTTGTTATGTGGTAATCTTGTTAACTTAATTGGTGGTCTTTTGCTGCCATTAGGTTGGAATAATCATGTACCTACCTACATCGGATGCACGCCAAAGAAAGGAAATAACCGAGGAGTTCTTTCACTATAGGCACCTCACCCAAACACTGCTGTGGGATCAATATTCTGCCTTTGAACATTGGGCTAAGATTGAGGTACCCACTTTCAAAATCCTTTTCTGATTAAACCCTACAAAAATGGTGGCATAAAGTTTTGATGTCTTTGTTATATTGGATGTAAATTTCAGGTTCCCAAGGACAAGGATGAGCTTGCAGCCCTGCAAGCAAGACTTAGGAAACGGTTTCCTGTCGATGAATACAACAAGGCACGACAAGCATTGGACCCGAACAAAATTCTCTCAAATAACAAGTTGGAAAAACTGTTCTCGTCATCAGATACGGTCTGAGATGCACATATATTCTTAGTGATCAATAATAAGTTCGGTTATCCAGTTGGGTAAGTGTTGTTTTGTTGGCTGTACTGTACATGTTCACACGAATGCTGCATTTGCATCTCCTTTGCTAAATTATTTATTTTGATTTTTTATATGCTACACTCATTTAGTAATTTCAACTAAAAGCAA

mRNA sequence

CTTGTAAGCTGTAACTCATCTCCGAACTTCGAATTTTCACTTCAAACAGCTCAATTGAATTCCTCTATTGGGCGACCCATAAACCCAAGAATCGATCTGAAGCCACTCCACCACACTATCAAATGCTCAAACTTCTCGCGCTCCGCCGATCCTTCCATTGCTTCCGCCTCCGCCGTCTCCAAATTCCACCCACCACCGCCATCTCCAAACCCCCTTTGAATCCCCCTCGCCCCTTTTCCACTGTTTCTTCTCCACCATCCTCCTCCTCCTTCGATTCCGAGCTTCGTAAGTACCTCGGCTATGGCGCTCTCGTCATTTTCTGCGGCGCCGCCACTTACTACTCCTTCCCTTTCCCCGAAAACGCCAAGCACAAGAAGGCCCAGATCTTTCGATACGCTCCTCTGCCTGAGGATCTTCACACTGTATCCAATTGGAGTGGAACTCATGAGGTTCTAACGAGAGTGTTTCACCAGCCGGAGAACTTGGAGCAACTTGAGCAAGTGGTAAAGGAGGCCAATGAAAAGAAGGCTAGGATTAGGCCTGTTGGCTCGGGGCTTTCGCCTAATGGGATTGGACTTTCGAAAATGGGGATGGTGAATTTGGCGTTAATGGATAAGGTTTTGGAAGTAGATAAGGAGAAGAAGAGGGTTAGGGTTCAAGCGGGAATAAGGGTTCAGCAGCTTGTAGATGGAATCAAGGAGTATGGGCTTACTTTGCAGAATTTTGCTTCTATCAGGGAGCAGCAAATTGGTGGCATCATTCAGGTCGGTGCACATGGTTCCGGTGCAAAATTGCCACCCATTGATGAGCAGGTTATTGCTATGAAATTGGTCACTCCTGCCAAGGGTACTATTGAGATTTCCAAAGATAAAGATCCAGAGCTTTTTTTTCTGGCTCGCTGTGGTCTTGGGGGGCTTGGAGTGGTTGCTGAAGTAACCCTCCAGTGTGTCGAGAGAGAGGAACTTGTGGAGCACACATACATTTCGAACATGAAAGACATCAAGAAGAACCACAAGAAATTATTGGGTGACAATAAGCATGTGAAATACCTGTATATCCCTTACACCGATGCTATTGTTGTTGTGACTTGCAATCCTATCTCAAAATGGAGAGGCCCTCCCAAATTCAAACCGAAATATACTAGTGAGGAAGCCATACAACATGTTCGTGATCTCTACTTGGAGTCCCTTAAAAAATACAGTGCTAGTGATGAAAGAGACATAAATGAATTTTCATTTACTGAGTTGCGTGATAAACTACTTGCCTTTGATCCTCTTAACAAAGAGCATGTAATGAAAGTGAATCAAGCAGAGGCTGAGTTCTGGAGGAAGTCGGAAGGATACAGAGTCGGATGGAGTGATGAAATTTTAGGTTTTGATTGTGGTGGCCAACAATGGGTCTCTGAAACTTGCTTTCCCGCCGGTACTCTTGCAAAACCTAACATGAAAGACATTGAATACATAGAGGAATTGAAGCAGCTTATCGAAAAAGAAAATATACCTGCACCTGCTCCTCTTGAGCAGCGGTGGACTGCTCGTAGTAAGAGCCCCATGAGTCCAGCTTCAAGCTCAGCTGAGGATGATATATTCTCTTGGGTTGGAATAATCATGTACCTACCTACATCGGATGCACGCCAAAGAAAGGAAATAACCGAGGAGTTCTTTCACTATAGGCACCTCACCCAAACACTGCTGTGGGATCAATATTCTGCCTTTGAACATTGGGCTAAGATTGAGGTTCCCAAGGACAAGGATGAGCTTGCAGCCCTGCAAGCAAGACTTAGGAAACGGTTTCCTGTCGATGAATACAACAAGGCACGACAAGCATTGGACCCGAACAAAATTCTCTCAAATAACAAGTTGGAAAAACTGTTCTCGTCATCAGATACGGTCTGAGATGCACATATATTCTTAGTGATCAATAATAAGTTCGGTTATCCAGTTGGGTAAGTGTTGTTTTGTTGGCTGTACTGTACATGTTCACACGAATGCTGCATTTGCATCTCCTTTGCTAAATTATTTATTTTGATTTTTTATATGCTACACTCATTTAGTAATTTCAACTAAAAGCAA

Coding sequence (CDS)

ATGCTCAAACTTCTCGCGCTCCGCCGATCCTTCCATTGCTTCCGCCTCCGCCGTCTCCAAATTCCACCCACCACCGCCATCTCCAAACCCCCTTTGAATCCCCCTCGCCCCTTTTCCACTGTTTCTTCTCCACCATCCTCCTCCTCCTTCGATTCCGAGCTTCGTAAGTACCTCGGCTATGGCGCTCTCGTCATTTTCTGCGGCGCCGCCACTTACTACTCCTTCCCTTTCCCCGAAAACGCCAAGCACAAGAAGGCCCAGATCTTTCGATACGCTCCTCTGCCTGAGGATCTTCACACTGTATCCAATTGGAGTGGAACTCATGAGGTTCTAACGAGAGTGTTTCACCAGCCGGAGAACTTGGAGCAACTTGAGCAAGTGGTAAAGGAGGCCAATGAAAAGAAGGCTAGGATTAGGCCTGTTGGCTCGGGGCTTTCGCCTAATGGGATTGGACTTTCGAAAATGGGGATGGTGAATTTGGCGTTAATGGATAAGGTTTTGGAAGTAGATAAGGAGAAGAAGAGGGTTAGGGTTCAAGCGGGAATAAGGGTTCAGCAGCTTGTAGATGGAATCAAGGAGTATGGGCTTACTTTGCAGAATTTTGCTTCTATCAGGGAGCAGCAAATTGGTGGCATCATTCAGGTCGGTGCACATGGTTCCGGTGCAAAATTGCCACCCATTGATGAGCAGGTTATTGCTATGAAATTGGTCACTCCTGCCAAGGGTACTATTGAGATTTCCAAAGATAAAGATCCAGAGCTTTTTTTTCTGGCTCGCTGTGGTCTTGGGGGGCTTGGAGTGGTTGCTGAAGTAACCCTCCAGTGTGTCGAGAGAGAGGAACTTGTGGAGCACACATACATTTCGAACATGAAAGACATCAAGAAGAACCACAAGAAATTATTGGGTGACAATAAGCATGTGAAATACCTGTATATCCCTTACACCGATGCTATTGTTGTTGTGACTTGCAATCCTATCTCAAAATGGAGAGGCCCTCCCAAATTCAAACCGAAATATACTAGTGAGGAAGCCATACAACATGTTCGTGATCTCTACTTGGAGTCCCTTAAAAAATACAGTGCTAGTGATGAAAGAGACATAAATGAATTTTCATTTACTGAGTTGCGTGATAAACTACTTGCCTTTGATCCTCTTAACAAAGAGCATGTAATGAAAGTGAATCAAGCAGAGGCTGAGTTCTGGAGGAAGTCGGAAGGATACAGAGTCGGATGGAGTGATGAAATTTTAGGTTTTGATTGTGGTGGCCAACAATGGGTCTCTGAAACTTGCTTTCCCGCCGGTACTCTTGCAAAACCTAACATGAAAGACATTGAATACATAGAGGAATTGAAGCAGCTTATCGAAAAAGAAAATATACCTGCACCTGCTCCTCTTGAGCAGCGGTGGACTGCTCGTAGTAAGAGCCCCATGAGTCCAGCTTCAAGCTCAGCTGAGGATGATATATTCTCTTGGGTTGGAATAATCATGTACCTACCTACATCGGATGCACGCCAAAGAAAGGAAATAACCGAGGAGTTCTTTCACTATAGGCACCTCACCCAAACACTGCTGTGGGATCAATATTCTGCCTTTGAACATTGGGCTAAGATTGAGGTTCCCAAGGACAAGGATGAGCTTGCAGCCCTGCAAGCAAGACTTAGGAAACGGTTTCCTGTCGATGAATACAACAAGGCACGACAAGCATTGGACCCGAACAAAATTCTCTCAAATAACAAGTTGGAAAAACTGTTCTCGTCATCAGATACGGTCTGA

Protein sequence

MLKLLALRRSFHCFRLRRLQIPPTTAISKPPLNPPRPFSTVSSPPSSSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSKMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGSGAKLPPIDEQVIAMKLVTPAKGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVEREELVEHTYISNMKDIKKNHKKLLGDNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYLESLKKYSASDERDINEFSFTELRDKLLAFDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMSPASSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV
Homology
BLAST of ClCG04G004060 vs. NCBI nr
Match: NP_001292704.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucumis sativus] >ADT80722.1 L-galactono-1,4-lactone dehydrogenase [Cucumis sativus])

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 562/590 (95.25%), Postives = 575/590 (97.46%), Query Frame = 0

Query: 1   MLKLLALRRSFHCFRLRRLQIPPTTAISKPPLNPPRPFSTVSSPPSSSSFDSELRKYLGY 60
           ML  L+LRRS H FR RRLQI PT AISKPP NPPRPFSTVS  PSSSSFDSELRKYLGY
Sbjct: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSFDSELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN 120
           GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQPEN
Sbjct: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPEN 120

Query: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSKMGMVNLALMDKVLEVDKEKKRVRVQA 180
           LEQLEQVVK+ANEKKARIRPVGSGLSPNGIGLS+MGMVNLALMDKVLEVDKEKKRVRVQA
Sbjct: 121 LEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGSGAKLPPIDEQVIAMKLVTPA 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHG+GAKLPPIDEQVIAMKLVTPA
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVEREELVEHTYISNMKDIKKNHKKL 300
           KGTIEISKDKDPELF LARCGLGGLGVVAEVTLQCVER+ELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LGDNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYLESLKKYS 360
           L DNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLY+ESLKKYS
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360

Query: 361 ASDERDINEFSFTELRDKLLAFDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
           AS+ERD+NE SFTELRDKLLA DPLNKEHV+KVNQAEAEFWRKSEGYRVGWSDEILGFDC
Sbjct: 361 ASEERDMNEISFTELRDKLLALDPLNKEHVIKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEK+NIPAPAPLEQRWTARSKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKKNIPAPAPLEQRWTARSKSPMSPA 480

Query: 481 SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540
           SS+AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP
Sbjct: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540

Query: 541 KDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 591
           KDKDELAALQARLRKRFPVDEYNKAR+ALDPNKILSNNKLEKLFSS+DTV
Sbjct: 541 KDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSTDTV 590

BLAST of ClCG04G004060 vs. NCBI nr
Match: KAE8637410.1 (hypothetical protein CSA_023387 [Cucumis sativus])

HSP 1 Score: 1128.6 bits (2918), Expect = 0.0e+00
Identity = 559/583 (95.88%), Postives = 571/583 (97.94%), Query Frame = 0

Query: 8   RRSFHCFRLRRLQIPPTTAISKPPLNPPRPFSTVSSPPSSSSFDSELRKYLGYGALVIFC 67
           RRS H FR RRLQI PT AISKPP NPPRPFSTVS  PSSSSFDSELRKYLGYGALVIFC
Sbjct: 240 RRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSFDSELRKYLGYGALVIFC 299

Query: 68  GAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENLEQLEQV 127
           GAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQPENLEQLEQV
Sbjct: 300 GAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPENLEQLEQV 359

Query: 128 VKEANEKKARIRPVGSGLSPNGIGLSKMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQL 187
           VK+ANEKKARIRPVGSGLSPNGIGLS+MGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQL
Sbjct: 360 VKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQL 419

Query: 188 VDGIKEYGLTLQNFASIREQQIGGIIQVGAHGSGAKLPPIDEQVIAMKLVTPAKGTIEIS 247
           VDGIKEYGLTLQNFASIREQQIGGIIQVGAHG+GAKLPPIDEQVIAMKLVTPAKGTIEIS
Sbjct: 420 VDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEIS 479

Query: 248 KDKDPELFFLARCGLGGLGVVAEVTLQCVEREELVEHTYISNMKDIKKNHKKLLGDNKHV 307
           KDKDPELF LARCGLGGLGVVAEVTLQCVER+ELVEHTYISNMKDIKKNHKKLL DNKHV
Sbjct: 480 KDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNKHV 539

Query: 308 KYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYLESLKKYSASDERDI 367
           KYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLY+ESLKKYSAS+ERD+
Sbjct: 540 KYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSASEERDM 599

Query: 368 NEFSFTELRDKLLAFDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVS 427
           NEFSFTELRDKLLA DPLNKEHV+KVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVS
Sbjct: 600 NEFSFTELRDKLLALDPLNKEHVIKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVS 659

Query: 428 ETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMSPASSSAEDD 487
           ETCFPAGTLAKPNMKDIEYIEELKQLIEK+NIPAPAPLEQRWTARSKSPMSPASS+AEDD
Sbjct: 660 ETCFPAGTLAKPNMKDIEYIEELKQLIEKKNIPAPAPLEQRWTARSKSPMSPASSTAEDD 719

Query: 488 IFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDELA 547
           IFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDELA
Sbjct: 720 IFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDELA 779

Query: 548 ALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 591
           ALQARLRKRFPVDEYNKAR+ALDPNKILSNNKLEKLFSS+DTV
Sbjct: 780 ALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSTDTV 822

BLAST of ClCG04G004060 vs. NCBI nr
Match: XP_038882838.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 1124.8 bits (2908), Expect = 0.0e+00
Identity = 561/590 (95.08%), Postives = 572/590 (96.95%), Query Frame = 0

Query: 1   MLKLLALRRSFHCFRLRRLQIPPTTAISKPPLNPPRPFSTVSSPPSSSSFDSELRKYLGY 60
           MLKLLALRRS  C RLR LQIPPTTAISKPPLNPPRPFSTVS PP SSSFDSELRKYLGY
Sbjct: 1   MLKLLALRRSHFC-RLRGLQIPPTTAISKPPLNPPRPFSTVSPPPQSSSFDSELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN 120
           G LVI CGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQPEN
Sbjct: 61  GTLVIICGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVQTRVFHQPEN 120

Query: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSKMGMVNLALMDKVLEVDKEKKRVRVQA 180
           LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLS+MGMVNLALMD VLEVDK++KRVRVQA
Sbjct: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDNVLEVDKDRKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGSGAKLPPIDEQVIAMKLVTPA 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHG+GAKLPPIDEQVIAMKLVTPA
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVEREELVEHTYISNMKDIKKNHKKL 300
           KGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVER+ELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LGDNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYLESLKKYS 360
           L DNKHVKYLYIPYTDAIVVVTCNPISKWRGPPK KPKYTSEEAIQHVRDLY+ESLKK+S
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKLKPKYTSEEAIQHVRDLYVESLKKHS 360

Query: 361 ASDERDINEFSFTELRDKLLAFDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
            SDE DINEFSFTELRDKLLA DPLNKEHV+KVNQAEAEFW+KSEGYRVGWSDEILGFDC
Sbjct: 361 VSDETDINEFSFTELRDKLLALDPLNKEHVIKVNQAEAEFWKKSEGYRVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAP+EQRWTA SKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPA 480

Query: 481 SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540
           SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHL QTLLWDQYSAFEHWAKIEVP
Sbjct: 481 SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLAQTLLWDQYSAFEHWAKIEVP 540

Query: 541 KDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 591
           KDKDELAALQARLRKRFPVDEYNKAR+ALDPNKILSNNKLEKL SSSDTV
Sbjct: 541 KDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLLSSSDTV 589

BLAST of ClCG04G004060 vs. NCBI nr
Match: NP_001284400.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucumis melo] >AAF64319.2 L-galactono-1,4-lactone dehydrogenase [Cucumis melo])

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 555/590 (94.07%), Postives = 571/590 (96.78%), Query Frame = 0

Query: 1   MLKLLALRRSFHCFRLRRLQIPPTTAISKPPLNPPRPFSTVSSPPSSSSFDSELRKYLGY 60
           ML  L+LRRS H FR RRLQI PT AISKPPLN PRPF TV SP  SSSFDSELRKYLGY
Sbjct: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTV-SPSPSSSFDSELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN 120
           GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPE+
Sbjct: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPED 120

Query: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSKMGMVNLALMDKVLEVDKEKKRVRVQA 180
           LEQLEQVVK+ANEKKAR+RPVGSGLSPNGIGLS++GMVN+ALMDKVLEVDKEKKRVRVQA
Sbjct: 121 LEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGSGAKLPPIDEQVIAMKLVTPA 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHG+GAKLPPIDEQVIAMKLVTPA
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVEREELVEHTYISNMKDIKKNHKKL 300
           KGTIEIS DKDPELFFLARCGLGGLGVVAEVTLQCVER+ELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LGDNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYLESLKKYS 360
           L DNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLY+ESLKKYS
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360

Query: 361 ASDERDINEFSFTELRDKLLAFDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
           AS+ERDINEFSFTELRDKLLA DPLNKEHVMKVNQAEAEFWRKSEGY+VGWSDEILGFDC
Sbjct: 361 ASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYKVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWT RSKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSPA 480

Query: 481 SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540
           SS+AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWD++SAFEHWAKIEVP
Sbjct: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEVP 540

Query: 541 KDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 591
           KDKDELAALQARLRKRFPVDE NKAR+ALDPNKILSNNKLEKLFSS D V
Sbjct: 541 KDKDELAALQARLRKRFPVDESNKARRALDPNKILSNNKLEKLFSSLDMV 589

BLAST of ClCG04G004060 vs. NCBI nr
Match: XP_023517263.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 553/590 (93.73%), Postives = 568/590 (96.27%), Query Frame = 0

Query: 1   MLKLLALRRSFHCFRLRRLQIPPTTAISKPPLNPPRPFSTVSSPPSSSSFDSELRKYLGY 60
           MLKLL+LRRS H    RR Q PPT AISKPPLNP R FSTVS PP SSS +SELRKYLGY
Sbjct: 1   MLKLLSLRRSLHLHH-RRPQFPPTVAISKPPLNPTRQFSTVSPPPPSSSSESELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN 120
           GALVI CGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN
Sbjct: 61  GALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN 120

Query: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSKMGMVNLALMDKVLEVDKEKKRVRVQA 180
           LEQLE+VVKE+N KKARIRPVGSGLSPNGIGLS+MGMVNLALMD+VLEVDKEKKRVRVQA
Sbjct: 121 LEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDRVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGSGAKLPPIDEQVIAMKLVTPA 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHG+GAKLPPIDEQVIAMKLVTPA
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVEREELVEHTYISNMKDIKKNHKKL 300
           KGTIEISKDKDPELF+LARCGLGGLGVVAEVTLQCVER+ELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISKDKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LGDNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYLESLKKYS 360
           L DNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKF+PKYTS+EAIQHVRDLY+ESLKKYS
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKKYS 360

Query: 361 ASDERDINEFSFTELRDKLLAFDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
           ASDERDINEFSFTELRDKLLA DPLNKEHV+KVNQAEAEFWRKSEGYRVGWSDEILGFDC
Sbjct: 361 ASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAP+EQRWTA SKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPA 480

Query: 481 SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540
           SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP
Sbjct: 481 SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540

Query: 541 KDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 591
           KDKDELAALQARLRKRFPVDEYNKAR  LDPNKILS+NKLEKLF SSD V
Sbjct: 541 KDKDELAALQARLRKRFPVDEYNKARGELDPNKILSSNKLEKLFVSSDVV 589

BLAST of ClCG04G004060 vs. ExPASy Swiss-Prot
Match: Q9SU56 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GLDH PE=1 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 1.2e-268
Identity = 453/572 (79.20%), Postives = 504/572 (88.11%), Query Frame = 0

Query: 27  ISKPPLNPPRPFSTVSSPPSSSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKA 86
           ++ PP  PPRP      PP +++ +++ RKY GY AL IF G ATY+SFPFPENAKHKKA
Sbjct: 42  LTPPPPPPPRP----PPPPPATASEAQFRKYAGYAALAIFSGVATYFSFPFPENAKHKKA 101

Query: 87  QIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENLEQLEQVVKEANEKKARIRPVGSGLS 146
           QIFRYAPLPEDLHTVSNWSGTHEV TR F+QPENL  LE +VKE++EKK RIRPVGSGLS
Sbjct: 102 QIFRYAPLPEDLHTVSNWSGTHEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLS 161

Query: 147 PNGIGLSKMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIRE 206
           PNGIGLS+ GMVNLALMDKVLEVDKEKKRV VQAGIRVQQLVD IK+YGLTLQNFASIRE
Sbjct: 162 PNGIGLSRSGMVNLALMDKVLEVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIRE 221

Query: 207 QQIGGIIQVGAHGSGAKLPPIDEQVIAMKLVTPAKGTIEISKDKDPELFFLARCGLGGLG 266
           QQIGGIIQVGAHG+GA+LPPIDEQVI+MKLVTPAKGTIE+S++KDPELF LARCGLGGLG
Sbjct: 222 QQIGGIIQVGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLG 281

Query: 267 VVAEVTLQCVEREELVEHTYISNMKDIKKNHKKLLGDNKHVKYLYIPYTDAIVVVTCNPI 326
           VVAEVTLQCV R ELVEHTY+SN+++IKKNHKKLL  NKHVKYLYIPYTD +VVVTCNP+
Sbjct: 282 VVAEVTLQCVARHELVEHTYVSNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPV 341

Query: 327 SKWRGPPKFKPKYTSEEAIQHVRDLYLESLKKYSASD---------ERDINEFSFTELRD 386
           SKW GPPK KPKYT++EA+QHVRDLY ES+ KY   D         E DI E SFTELRD
Sbjct: 342 SKWSGPPKDKPKYTTDEAVQHVRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRD 401

Query: 387 KLLAFDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLA 446
           KLLA DPLN  HV KVNQAEAEFW+KSEGYRVGWSDEILGFDCGGQQWVSE+CFPAGTLA
Sbjct: 402 KLLALDPLNDVHVAKVNQAEAEFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLA 461

Query: 447 KPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMSPASSSAEDDIFSWVGIIMY 506
            P+MKD+EYIEELK+LIEKE IPAPAP+EQRWTARSKSP+SPA S++EDDIFSWVGIIMY
Sbjct: 462 NPSMKDLEYIEELKKLIEKEAIPAPAPIEQRWTARSKSPISPAFSTSEDDIFSWVGIIMY 521

Query: 507 LPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDELAALQARLRKRF 566
           LPT+D RQRK+IT+EFFHYRHLTQ  LWDQ+SA+EHWAKIE+PKDK+EL ALQAR+RKRF
Sbjct: 522 LPTADPRQRKDITDEFFHYRHLTQKQLWDQFSAYEHWAKIEIPKDKEELEALQARIRKRF 581

Query: 567 PVDEYNKARQALDPNKILSNNKLEKLFSSSDT 590
           PVD YNKAR+ LDPN+ILSNN +EKLF  S T
Sbjct: 582 PVDAYNKARRELDPNRILSNNMVEKLFPVSTT 609

BLAST of ClCG04G004060 vs. ExPASy Swiss-Prot
Match: O47881 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Brassica oleracea OX=3712 PE=1 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 4.7e-268
Identity = 467/601 (77.70%), Postives = 512/601 (85.19%), Query Frame = 0

Query: 1   MLKLLALRRSFHCFRLRRLQIPP--TTAISKPPLNPPRPFSTVSSPP-SSSSFDSELRKY 60
           ML+ L LRRS    R  R   PP  T   S   L P  P      PP SSS+ + E RKY
Sbjct: 1   MLRSLLLRRS--NARSLRPPFPPLRTLCTSGQTLTPAPPPPPPPPPPISSSASEKEFRKY 60

Query: 61  LGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQ 120
            GY AL +F GAATY+SFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TR F+Q
Sbjct: 61  AGYAALALFSGAATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVQTRNFNQ 120

Query: 121 PENLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSKMGMVNLALMDKVLEVDKEKKRVR 180
           PE L  LE +VKEA+EKK RIRPVGSGLSPNGIGLS+ GMVNLALMDKVLEVDKEKKRVR
Sbjct: 121 PETLADLEALVKEAHEKKNRIRPVGSGLSPNGIGLSRSGMVNLALMDKVLEVDKEKKRVR 180

Query: 181 VQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGSGAKLPPIDEQVIAMKLV 240
           VQAGIRVQQLVD I+EYGLTLQNFASIREQQIGGIIQVGAHG+GA+LPPIDEQVI MKLV
Sbjct: 181 VQAGIRVQQLVDAIQEYGLTLQNFASIREQQIGGIIQVGAHGTGARLPPIDEQVIGMKLV 240

Query: 241 TPAKGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVEREELVEHTYISNMKDIKKNH 300
           TPAKGTIE+SKD DPELF LARCGLGGLGVVAEVTLQCVER+EL+EHTY+S +++IKKNH
Sbjct: 241 TPAKGTIELSKDNDPELFHLARCGLGGLGVVAEVTLQCVERQELLEHTYVSTLEEIKKNH 300

Query: 301 KKLLGDNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYLESLK 360
           KKLL  NKHVKYLYIPYTD +VVVTCNP+SKW G PK KPKYT+EEA++HVRDLY ES+ 
Sbjct: 301 KKLLSTNKHVKYLYIPYTDTVVVVTCNPVSKWSGAPKDKPKYTTEEALKHVRDLYRESIV 360

Query: 361 KYSASD---------ERDINEFSFTELRDKLLAFDPLNKEHVMKVNQAEAEFWRKSEGYR 420
           KY   D         E DINE SFTELRDKL+A DPLN  HV KVNQAEAEFW+KSEGYR
Sbjct: 361 KYRVQDSSKKTPDSREPDINELSFTELRDKLIALDPLNDVHVGKVNQAEAEFWKKSEGYR 420

Query: 421 VGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQR 480
           VGWSDEILGFDCGGQQWVSETCFPAGTLAKP+MKD+EYIE+LK+LI+KE IPAP+P+EQR
Sbjct: 421 VGWSDEILGFDCGGQQWVSETCFPAGTLAKPSMKDLEYIEQLKELIQKEAIPAPSPIEQR 480

Query: 481 WTARSKSPMSPASSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQY 540
           WT RSKSPMSPA S+AE+DIFSWVGIIMYLPT+D RQRK+IT+EFFHYRHLTQ  LWDQY
Sbjct: 481 WTGRSKSPMSPAFSTAEEDIFSWVGIIMYLPTADPRQRKDITDEFFHYRHLTQAKLWDQY 540

Query: 541 SAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSD 590
           SA+EHWAKIE+PKDK+EL ALQ RLRKRFPVD YNKAR+ LDPN+ILSNN +EKLF  S 
Sbjct: 541 SAYEHWAKIEIPKDKEELEALQERLRKRFPVDAYNKARRELDPNRILSNNMVEKLFPVSK 599

BLAST of ClCG04G004060 vs. ExPASy Swiss-Prot
Match: Q2RAP0 (L-galactono-1,4-lactone dehydrogenase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=GLDH1 PE=2 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 1.1e-240
Identity = 415/581 (71.43%), Postives = 487/581 (83.82%), Query Frame = 0

Query: 7   LRRSFHCFRLRRLQIPPTTAISKPPLNPPRPFSTVSSPPSSSSFDSELRKYLGYGALVIF 66
           +RR      LRR    P+   S   L+  R  S  S  P+S   D++LRKY GY  L++ 
Sbjct: 1   MRRLLLAGILRRASSSPS---SHHHLHLVRALSASSPLPAS---DADLRKYAGYALLLLG 60

Query: 67  CGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENLEQLEQ 126
           CGAATYYSFP P +A HKKA  F+YAPLP+DLH VSNWS THEV TRV  QP++L  L  
Sbjct: 61  CGAATYYSFPLPPDALHKKAVPFKYAPLPDDLHAVSNWSATHEVHTRVLLQPDSLPALHD 120

Query: 127 VVKEANEKKARIRPVGSGLSPNGIGLSKMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQ 186
            +  A+ +  ++RP+GSGLSPNG+ LS+ GMVNLALMDKVL VD +KK V VQAGIRV +
Sbjct: 121 ALAAAHGECRKLRPLGSGLSPNGLALSRAGMVNLALMDKVLGVDAKKKTVTVQAGIRVAE 180

Query: 187 LVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGSGAKLPPIDEQVIAMKLVTPAKGTIEI 246
           LVD ++E+GLTLQNFASIREQQ+GGIIQVGAHG+GA+LPPIDEQVI+MKLVTPAKGTIE+
Sbjct: 181 LVDALREHGLTLQNFASIREQQVGGIIQVGAHGTGARLPPIDEQVISMKLVTPAKGTIEL 240

Query: 247 SKDKDPELFFLARCGLGGLGVVAEVTLQCVEREELVEHTYISNMKDIKKNHKKLLGDNKH 306
           S++KDP+LF+LARCGLGGLGVVAEVTLQCVER +L+EHT++SN  ++KKNHKK L +NKH
Sbjct: 241 SREKDPDLFYLARCGLGGLGVVAEVTLQCVERHQLIEHTFVSNADEVKKNHKKWLSENKH 300

Query: 307 VKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYLESLKKY---SASD 366
           +KYL+IPYTD +VVV CNP S+WR  PKF  KY  +EAIQHVRDLY ESLKKY   + S+
Sbjct: 301 IKYLWIPYTDTVVVVQCNPPSRWR-TPKFTSKYGKDEAIQHVRDLYHESLKKYRTKAESN 360

Query: 367 ERDINEFSFTELRDKLLAFDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQ 426
           + ++++ SFTELRD+LL  DPL+K+HV+++N+AEAE+W+KSEGYR+GWSDEILGFDCGGQ
Sbjct: 361 DPEVDQLSFTELRDRLLTLDPLDKDHVIRINKAEAEYWKKSEGYRMGWSDEILGFDCGGQ 420

Query: 427 QWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMSPASSS 486
           QWVSETCFPAGTLAKPNMKD++YIEEL QLIEKE+IPAPAP+EQRWTA S+SPMSPASSS
Sbjct: 421 QWVSETCFPAGTLAKPNMKDLDYIEELLQLIEKEDIPAPAPIEQRWTACSRSPMSPASSS 480

Query: 487 AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDK 546
            EDDIFSWVGIIMYLPTSDARQRKEITEEFF+YR  TQT LWD YSA+EHWAKIEVPKDK
Sbjct: 481 QEDDIFSWVGIIMYLPTSDARQRKEITEEFFNYRSKTQTNLWDGYSAYEHWAKIEVPKDK 540

Query: 547 DELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLF 585
           DELA LQARLRKRFPVD YNKAR  LDPNK+LSN KLEKLF
Sbjct: 541 DELAELQARLRKRFPVDAYNKARMELDPNKVLSNAKLEKLF 574

BLAST of ClCG04G004060 vs. ExPASy Swiss-Prot
Match: Q2QXY1 (L-galactono-1,4-lactone dehydrogenase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=GLDH2 PE=2 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 4.2e-240
Identity = 413/581 (71.08%), Postives = 488/581 (83.99%), Query Frame = 0

Query: 7   LRRSFHCFRLRRLQIPPTTAISKPPLNPPRPFSTVSSPPSSSSFDSELRKYLGYGALVIF 66
           +RR      LRR    P+   S   L+  R  S  S  P+S   D++LRKY GY  L++ 
Sbjct: 1   MRRLLLAGILRRASSSPS---SHHHLHLVRALSASSPLPAS---DADLRKYAGYALLLLG 60

Query: 67  CGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENLEQLEQ 126
           CGAATYYSFP P +A HKKA  F+YAPLP+DLH VSNWS THEV TRV  QP++L  L  
Sbjct: 61  CGAATYYSFPLPPDALHKKAVPFKYAPLPDDLHAVSNWSATHEVHTRVLLQPDSLPVLHD 120

Query: 127 VVKEANEKKARIRPVGSGLSPNGIGLSKMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQ 186
            +  A+ ++ ++RP+GSGLSPNG+ LS+ GMVNLALMDKVL+VD +KK V VQAGIRV +
Sbjct: 121 ALAAAHGERRKLRPLGSGLSPNGLALSRAGMVNLALMDKVLDVDAKKKTVTVQAGIRVAE 180

Query: 187 LVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGSGAKLPPIDEQVIAMKLVTPAKGTIEI 246
           LVD ++E+GLTLQNFASIREQQ+GGIIQVGAHG+GA+LPPIDEQVI+MKLVTPAKGTIE+
Sbjct: 181 LVDTLREHGLTLQNFASIREQQVGGIIQVGAHGTGARLPPIDEQVISMKLVTPAKGTIEL 240

Query: 247 SKDKDPELFFLARCGLGGLGVVAEVTLQCVEREELVEHTYISNMKDIKKNHKKLLGDNKH 306
           S++KDP+LF+LARCGLGGLGVVAEVTLQCVER +L+EHT++S+  ++KKNHKK L +NKH
Sbjct: 241 SREKDPDLFYLARCGLGGLGVVAEVTLQCVERHQLIEHTFVSSADEVKKNHKKWLSENKH 300

Query: 307 VKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYLESLKKY---SASD 366
           +KYL+IPYTD +VVV CNP S+WR  PKF  KY  +EAIQHVRDLY ESLKKY   + S+
Sbjct: 301 IKYLWIPYTDTVVVVQCNPPSRWR-TPKFTSKYGKDEAIQHVRDLYRESLKKYRTKAESN 360

Query: 367 ERDINEFSFTELRDKLLAFDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQ 426
           + ++++ SFTELRD+LLA DPL+K+HV+++N+AEAE+W+KSEGYR+GWSDEILGFDCGGQ
Sbjct: 361 DPEVDQLSFTELRDRLLALDPLDKDHVIRINKAEAEYWKKSEGYRMGWSDEILGFDCGGQ 420

Query: 427 QWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMSPASSS 486
           QWVSETCFPAGTLAKPNMKD++YIEEL QLIEKE+IPAPAP+EQRWTA S+SPMSPASSS
Sbjct: 421 QWVSETCFPAGTLAKPNMKDLDYIEELLQLIEKEDIPAPAPIEQRWTACSRSPMSPASSS 480

Query: 487 AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDK 546
            EDDIFSWVGIIMYLPTSDARQRKEITEEFF+YR  TQT LWD YSA+EHWAKIEVPKDK
Sbjct: 481 QEDDIFSWVGIIMYLPTSDARQRKEITEEFFNYRSKTQTNLWDGYSAYEHWAKIEVPKDK 540

Query: 547 DELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLF 585
           DEL  L ARLRKRFPVD YNKAR  LDPNK+LSN KLEKLF
Sbjct: 541 DELTELLARLRKRFPVDAYNKARMELDPNKVLSNAKLEKLF 574

BLAST of ClCG04G004060 vs. ExPASy Swiss-Prot
Match: P10867 (L-gulonolactone oxidase OS=Rattus norvegicus OX=10116 GN=Gulo PE=1 SV=3)

HSP 1 Score: 156.8 bits (395), Expect = 7.8e-37
Identity = 128/484 (26.45%), Postives = 224/484 (46.28%), Query Frame = 0

Query: 103 NWSGTHEVLTRVFHQPENLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSKMGMVNLAL 162
           NW+ T+     V++QP ++E++ +V+  A E+K +++ VG G SP+ I  +   M+++  
Sbjct: 12  NWAKTYGCSPEVYYQPTSVEEVREVLALAREQKKKVKVVGGGHSPSDIACTDGFMIHMGK 71

Query: 163 MDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGSGA 222
           M++VL+VDKEKK++ V+AGI +  L   + E+GL + N  ++ +  + G+I  G H +G 
Sbjct: 72  MNRVLQVDKEKKQITVEAGILLADLHPQLDEHGLAMSNLGAVSDVTVAGVIGSGTHNTGI 131

Query: 223 KLPPIDEQVIAMKLVTPAKGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVEREELV 282
           K   +  QV+A+ L+T     +E S+ ++ ++F  AR  LG LG++  VTLQCV +  L 
Sbjct: 132 KHGILATQVVALTLMTADGEVLECSESRNADVFQAARVHLGCLGIILTVTLQCVPQFHLQ 191

Query: 283 EHTYISNMKDIKKNHKKLLGDNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSE 342
           E ++ S +K++  N    L  +++ ++L+ P+T+ + ++  +  +K    P     +  +
Sbjct: 192 ETSFPSTLKEVLDNLDSHLKRSEYFRFLWFPHTENVSIIYQDHTNK---APSSASNWFWD 251

Query: 343 EAIQHVRDLYLESLKKYSASDERDINEFSFTELRDKLLAFDPLNKEHVMKVNQAEAEFWR 402
            AI      +L     Y       IN F F  L                        F  
Sbjct: 252 YAIGFYLLEFLLWTSTYLPCLVGWINRFFFWML------------------------FNC 311

Query: 403 KSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKE-NIPA 462
           K E   +  S +I  ++C  +Q V +        A P  K  E + ELK ++E    + A
Sbjct: 312 KKESSNL--SHKIFTYECRFKQHVQD-------WAIPREKTKEALLELKAMLEAHPKVVA 371

Query: 463 PAPLEQRWTARSKSPMSPASSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQ 522
             P+E R+T      +SP           ++ IIMY P      R +          L  
Sbjct: 372 HYPVEVRFTRGDDILLSPCFQRDS----CYMNIIMYRPYGKDVPRLDY--------WLAY 431

Query: 523 TLLWDQYSAFEHWAKIEVPKDKDELAALQARLRKRFPV-DEYNKARQALDPNKILSNNKL 582
             +  ++    HWAK      KD          + +P   ++   R+ LDP  +  N+ L
Sbjct: 432 ETIMKKFGGRPHWAKAHNCTQKD--------FEEMYPTFHKFCDIREKLDPTGMFLNSYL 439

Query: 583 EKLF 585
           EK+F
Sbjct: 492 EKVF 439

BLAST of ClCG04G004060 vs. ExPASy TrEMBL
Match: E7E016 (L-galactono-1,4-lactone dehydrogenase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 562/590 (95.25%), Postives = 575/590 (97.46%), Query Frame = 0

Query: 1   MLKLLALRRSFHCFRLRRLQIPPTTAISKPPLNPPRPFSTVSSPPSSSSFDSELRKYLGY 60
           ML  L+LRRS H FR RRLQI PT AISKPP NPPRPFSTVS  PSSSSFDSELRKYLGY
Sbjct: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSFDSELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN 120
           GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQPEN
Sbjct: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPEN 120

Query: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSKMGMVNLALMDKVLEVDKEKKRVRVQA 180
           LEQLEQVVK+ANEKKARIRPVGSGLSPNGIGLS+MGMVNLALMDKVLEVDKEKKRVRVQA
Sbjct: 121 LEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGSGAKLPPIDEQVIAMKLVTPA 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHG+GAKLPPIDEQVIAMKLVTPA
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVEREELVEHTYISNMKDIKKNHKKL 300
           KGTIEISKDKDPELF LARCGLGGLGVVAEVTLQCVER+ELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LGDNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYLESLKKYS 360
           L DNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLY+ESLKKYS
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360

Query: 361 ASDERDINEFSFTELRDKLLAFDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
           AS+ERD+NE SFTELRDKLLA DPLNKEHV+KVNQAEAEFWRKSEGYRVGWSDEILGFDC
Sbjct: 361 ASEERDMNEISFTELRDKLLALDPLNKEHVIKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEK+NIPAPAPLEQRWTARSKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKKNIPAPAPLEQRWTARSKSPMSPA 480

Query: 481 SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540
           SS+AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP
Sbjct: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540

Query: 541 KDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 591
           KDKDELAALQARLRKRFPVDEYNKAR+ALDPNKILSNNKLEKLFSS+DTV
Sbjct: 541 KDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSTDTV 590

BLAST of ClCG04G004060 vs. ExPASy TrEMBL
Match: Q9M4U9 (L-galactono-1,4-lactone dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103497260 PE=2 SV=2)

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 555/590 (94.07%), Postives = 571/590 (96.78%), Query Frame = 0

Query: 1   MLKLLALRRSFHCFRLRRLQIPPTTAISKPPLNPPRPFSTVSSPPSSSSFDSELRKYLGY 60
           ML  L+LRRS H FR RRLQI PT AISKPPLN PRPF TV SP  SSSFDSELRKYLGY
Sbjct: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTV-SPSPSSSFDSELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN 120
           GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPE+
Sbjct: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPED 120

Query: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSKMGMVNLALMDKVLEVDKEKKRVRVQA 180
           LEQLEQVVK+ANEKKAR+RPVGSGLSPNGIGLS++GMVN+ALMDKVLEVDKEKKRVRVQA
Sbjct: 121 LEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGSGAKLPPIDEQVIAMKLVTPA 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHG+GAKLPPIDEQVIAMKLVTPA
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVEREELVEHTYISNMKDIKKNHKKL 300
           KGTIEIS DKDPELFFLARCGLGGLGVVAEVTLQCVER+ELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LGDNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYLESLKKYS 360
           L DNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLY+ESLKKYS
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360

Query: 361 ASDERDINEFSFTELRDKLLAFDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
           AS+ERDINEFSFTELRDKLLA DPLNKEHVMKVNQAEAEFWRKSEGY+VGWSDEILGFDC
Sbjct: 361 ASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYKVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWT RSKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSPA 480

Query: 481 SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540
           SS+AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWD++SAFEHWAKIEVP
Sbjct: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEVP 540

Query: 541 KDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 591
           KDKDELAALQARLRKRFPVDE NKAR+ALDPNKILSNNKLEKLFSS D V
Sbjct: 541 KDKDELAALQARLRKRFPVDESNKARRALDPNKILSNNKLEKLFSSLDMV 589

BLAST of ClCG04G004060 vs. ExPASy TrEMBL
Match: A0A6J1HRG8 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111465966 PE=4 SV=1)

HSP 1 Score: 1102.4 bits (2850), Expect = 0.0e+00
Identity = 550/590 (93.22%), Postives = 567/590 (96.10%), Query Frame = 0

Query: 1   MLKLLALRRSFHCFRLRRLQIPPTTAISKPPLNPPRPFSTVSSPPSSSSFDSELRKYLGY 60
           MLKLL+LRRS H    RR Q PPT AISKPPLNP R FSTVS PP SSS +SELRKYLGY
Sbjct: 1   MLKLLSLRRSLHLHH-RRPQFPPTVAISKPPLNPTRQFSTVSPPPPSSSSESELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN 120
           GALVIFCGA TYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQP+N
Sbjct: 61  GALVIFCGATTYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVHTRVFHQPDN 120

Query: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSKMGMVNLALMDKVLEVDKEKKRVRVQA 180
           LEQL++VVKEAN KKARIRPVGSGLSPNGIGLS+MGMVNLALMDKVLEVDKEKKRVRVQA
Sbjct: 121 LEQLQEVVKEANVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGSGAKLPPIDEQVIAMKLVTPA 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHG+GAKLPPIDEQVIAMKLVTPA
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVEREELVEHTYISNMKDIKKNHKKL 300
           KGTIEISK+KDPELF+LARCGLGGLGVVAEVTLQCVER+ELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISKEKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LGDNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYLESLKKYS 360
           L DNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKF+PKYTS+EAIQHVRDLY+ESLKKYS
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKKYS 360

Query: 361 ASDERDINEFSFTELRDKLLAFDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
           ASDERDINEFSFTELRDKLLA DPLNKEHV+KVNQAEAEFW KSEGYRVGWSDEILGFDC
Sbjct: 361 ASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWWKSEGYRVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAP+EQRWTA SKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPA 480

Query: 481 SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540
           SSS+EDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP
Sbjct: 481 SSSSEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540

Query: 541 KDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 591
           KDKDELAALQARLRKRFPVDEYNKARQ LDPNKILS+NKLEKLF SSD V
Sbjct: 541 KDKDELAALQARLRKRFPVDEYNKARQELDPNKILSSNKLEKLFVSSDVV 589

BLAST of ClCG04G004060 vs. ExPASy TrEMBL
Match: A0A6J1EEF4 (L-galactono-1,4-lactone dehydrogenase, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111432481 PE=4 SV=1)

HSP 1 Score: 1099.7 bits (2843), Expect = 0.0e+00
Identity = 546/590 (92.54%), Postives = 568/590 (96.27%), Query Frame = 0

Query: 1   MLKLLALRRSFHCFRLRRLQIPPTTAISKPPLNPPRPFSTVSSPPSSSSFDSELRKYLGY 60
           ML+LL+LRRS H    RR   PPT AIS+PPLNP R FSTVS PP SSS +SELRKY+GY
Sbjct: 1   MLRLLSLRRSLHLHH-RRPHFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGY 60

Query: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN 120
           GALVI CGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN
Sbjct: 61  GALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN 120

Query: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSKMGMVNLALMDKVLEVDKEKKRVRVQA 180
           LEQLE+VVKE+N KKARIRPVGSGLSPNGIGLS+MGMVNLALMD+VLEVDKEKKRVRVQA
Sbjct: 121 LEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGSGAKLPPIDEQVIAMKLVTPA 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHG+GAKLPPIDEQVIAMKLVTPA
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVEREELVEHTYISNMKDIKKNHKKL 300
           KGTIEIS++KDPELF+LARCGLGGLGVVAEVTLQCVER+ELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LGDNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYLESLKKYS 360
           L DNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKF+PKYTS+EAIQHVRDLY+ESLKKYS
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKKYS 360

Query: 361 ASDERDINEFSFTELRDKLLAFDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
           ASDERDINEFSFTELRDKLLA DPLNKEHV+KVNQAEAEFWRKSEGYRVGWSDEILGFDC
Sbjct: 361 ASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAP+EQRWTA SKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPA 480

Query: 481 SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540
           SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP
Sbjct: 481 SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540

Query: 541 KDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 591
           KDKDELAALQARLRKRFPVDEYNKAR+ LDPNKILS+NKLEK+F SSD V
Sbjct: 541 KDKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 589

BLAST of ClCG04G004060 vs. ExPASy TrEMBL
Match: A0A6J1DK81 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111021307 PE=4 SV=1)

HSP 1 Score: 1057.7 bits (2734), Expect = 1.7e-305
Identity = 528/593 (89.04%), Postives = 556/593 (93.76%), Query Frame = 0

Query: 1   MLKLLALRRSFHCFRLRRLQIPPTTAISKPPLN---PPRPFSTVSSPPSSSSFDSELRKY 60
           ML++L LRRS      RRL  PP  AIS+ PLN    P  FSTVS PP SSSFDSELRKY
Sbjct: 1   MLRILPLRRSH-----RRLLSPPAAAISRTPLNHHHHPPSFSTVSPPPPSSSFDSELRKY 60

Query: 61  LGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQ 120
           LGYGALV+FCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVF Q
Sbjct: 61  LGYGALVVFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVQTRVFQQ 120

Query: 121 PENLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSKMGMVNLALMDKVLEVDKEKKRVR 180
           PENLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLS+MGMVNLALMDKVLEVDKEKKRVR
Sbjct: 121 PENLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVR 180

Query: 181 VQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGSGAKLPPIDEQVIAMKLV 240
           VQAGIRVQQLVDGIKE+GLTLQNFASIREQQIGGIIQVGAHG+G KLPPIDEQVIAMKLV
Sbjct: 181 VQAGIRVQQLVDGIKEHGLTLQNFASIREQQIGGIIQVGAHGTGPKLPPIDEQVIAMKLV 240

Query: 241 TPAKGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVEREELVEHTYISNMKDIKKNH 300
           TPAKGTIEISK+KDPELF+LARCGLGGLGVVAEVTLQCVER+ELVEHTYISNM DIKKNH
Sbjct: 241 TPAKGTIEISKEKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMNDIKKNH 300

Query: 301 KKLLGDNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYLESLK 360
           KKLL DNKHVKYLYIPYTDA+VVVTCNPISKWRGPPK KPKYTS+EAIQHVRDLY ESLK
Sbjct: 301 KKLLTDNKHVKYLYIPYTDAVVVVTCNPISKWRGPPKLKPKYTSDEAIQHVRDLYAESLK 360

Query: 361 KYSASDERDINEFSFTELRDKLLAFDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILG 420
           KYS SDE DINE+S+TELRDKLLA DPLNKEHV KVNQAEAEFWRKSEGY+VGWSDEILG
Sbjct: 361 KYSTSDETDINEYSYTELRDKLLALDPLNKEHVTKVNQAEAEFWRKSEGYKVGWSDEILG 420

Query: 421 FDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPM 480
           FDCGGQQWVSETCFPAGTLAKP+MKDI+YIE+LKQLIEKENIPAPAP+EQRWTA SKSPM
Sbjct: 421 FDCGGQQWVSETCFPAGTLAKPSMKDIKYIEDLKQLIEKENIPAPAPIEQRWTACSKSPM 480

Query: 481 SPASSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKI 540
           SPASS A+DD+FSWVGIIMYLPT DARQRKEITEEFFHYRHLTQ+ LWDQYSAFEHWAKI
Sbjct: 481 SPASSLAQDDLFSWVGIIMYLPTLDARQRKEITEEFFHYRHLTQSQLWDQYSAFEHWAKI 540

Query: 541 EVPKDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 591
           EVP+DK+ELAALQARLRKRFPVDE+NKA++ALDPNKILSNNKLEKLF S+D V
Sbjct: 541 EVPRDKEELAALQARLRKRFPVDEFNKAQRALDPNKILSNNKLEKLFPSNDAV 588

BLAST of ClCG04G004060 vs. TAIR 10
Match: AT3G47930.1 (L-galactono-1,4-lactone dehydrogenase )

HSP 1 Score: 926.8 bits (2394), Expect = 8.8e-270
Identity = 453/572 (79.20%), Postives = 504/572 (88.11%), Query Frame = 0

Query: 27  ISKPPLNPPRPFSTVSSPPSSSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKA 86
           ++ PP  PPRP      PP +++ +++ RKY GY AL IF G ATY+SFPFPENAKHKKA
Sbjct: 42  LTPPPPPPPRP----PPPPPATASEAQFRKYAGYAALAIFSGVATYFSFPFPENAKHKKA 101

Query: 87  QIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENLEQLEQVVKEANEKKARIRPVGSGLS 146
           QIFRYAPLPEDLHTVSNWSGTHEV TR F+QPENL  LE +VKE++EKK RIRPVGSGLS
Sbjct: 102 QIFRYAPLPEDLHTVSNWSGTHEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLS 161

Query: 147 PNGIGLSKMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIRE 206
           PNGIGLS+ GMVNLALMDKVLEVDKEKKRV VQAGIRVQQLVD IK+YGLTLQNFASIRE
Sbjct: 162 PNGIGLSRSGMVNLALMDKVLEVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIRE 221

Query: 207 QQIGGIIQVGAHGSGAKLPPIDEQVIAMKLVTPAKGTIEISKDKDPELFFLARCGLGGLG 266
           QQIGGIIQVGAHG+GA+LPPIDEQVI+MKLVTPAKGTIE+S++KDPELF LARCGLGGLG
Sbjct: 222 QQIGGIIQVGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLG 281

Query: 267 VVAEVTLQCVEREELVEHTYISNMKDIKKNHKKLLGDNKHVKYLYIPYTDAIVVVTCNPI 326
           VVAEVTLQCV R ELVEHTY+SN+++IKKNHKKLL  NKHVKYLYIPYTD +VVVTCNP+
Sbjct: 282 VVAEVTLQCVARHELVEHTYVSNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPV 341

Query: 327 SKWRGPPKFKPKYTSEEAIQHVRDLYLESLKKYSASD---------ERDINEFSFTELRD 386
           SKW GPPK KPKYT++EA+QHVRDLY ES+ KY   D         E DI E SFTELRD
Sbjct: 342 SKWSGPPKDKPKYTTDEAVQHVRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRD 401

Query: 387 KLLAFDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLA 446
           KLLA DPLN  HV KVNQAEAEFW+KSEGYRVGWSDEILGFDCGGQQWVSE+CFPAGTLA
Sbjct: 402 KLLALDPLNDVHVAKVNQAEAEFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLA 461

Query: 447 KPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMSPASSSAEDDIFSWVGIIMY 506
            P+MKD+EYIEELK+LIEKE IPAPAP+EQRWTARSKSP+SPA S++EDDIFSWVGIIMY
Sbjct: 462 NPSMKDLEYIEELKKLIEKEAIPAPAPIEQRWTARSKSPISPAFSTSEDDIFSWVGIIMY 521

Query: 507 LPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDELAALQARLRKRF 566
           LPT+D RQRK+IT+EFFHYRHLTQ  LWDQ+SA+EHWAKIE+PKDK+EL ALQAR+RKRF
Sbjct: 522 LPTADPRQRKDITDEFFHYRHLTQKQLWDQFSAYEHWAKIEIPKDKEELEALQARIRKRF 581

Query: 567 PVDEYNKARQALDPNKILSNNKLEKLFSSSDT 590
           PVD YNKAR+ LDPN+ILSNN +EKLF  S T
Sbjct: 582 PVDAYNKARRELDPNRILSNNMVEKLFPVSTT 609

BLAST of ClCG04G004060 vs. TAIR 10
Match: AT3G47930.2 (L-galactono-1,4-lactone dehydrogenase )

HSP 1 Score: 737.3 bits (1902), Expect = 9.9e-213
Identity = 362/456 (79.39%), Postives = 399/456 (87.50%), Query Frame = 0

Query: 27  ISKPPLNPPRPFSTVSSPPSSSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKA 86
           ++ PP  PPRP      PP +++ +++ RKY GY AL IF G ATY+SFPFPENAKHKKA
Sbjct: 42  LTPPPPPPPRP----PPPPPATASEAQFRKYAGYAALAIFSGVATYFSFPFPENAKHKKA 101

Query: 87  QIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENLEQLEQVVKEANEKKARIRPVGSGLS 146
           QIFRYAPLPEDLHTVSNWSGTHEV TR F+QPENL  LE +VKE++EKK RIRPVGSGLS
Sbjct: 102 QIFRYAPLPEDLHTVSNWSGTHEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLS 161

Query: 147 PNGIGLSKMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIRE 206
           PNGIGLS+ GMVNLALMDKVLEVDKEKKRV VQAGIRVQQLVD IK+YGLTLQNFASIRE
Sbjct: 162 PNGIGLSRSGMVNLALMDKVLEVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIRE 221

Query: 207 QQIGGIIQVGAHGSGAKLPPIDEQVIAMKLVTPAKGTIEISKDKDPELFFLARCGLGGLG 266
           QQIGGIIQVGAHG+GA+LPPIDEQVI+MKLVTPAKGTIE+S++KDPELF LARCGLGGLG
Sbjct: 222 QQIGGIIQVGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLG 281

Query: 267 VVAEVTLQCVEREELVEHTYISNMKDIKKNHKKLLGDNKHVKYLYIPYTDAIVVVTCNPI 326
           VVAEVTLQCV R ELVEHTY+SN+++IKKNHKKLL  NKHVKYLYIPYTD +VVVTCNP+
Sbjct: 282 VVAEVTLQCVARHELVEHTYVSNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPV 341

Query: 327 SKWRGPPKFKPKYTSEEAIQHVRDLYLESLKKYSASD---------ERDINEFSFTELRD 386
           SKW GPPK KPKYT++EA+QHVRDLY ES+ KY   D         E DI E SFTELRD
Sbjct: 342 SKWSGPPKDKPKYTTDEAVQHVRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRD 401

Query: 387 KLLAFDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLA 446
           KLLA DPLN  HV KVNQAEAEFW+KSEGYRVGWSDEILGFDCGGQQWVSE+CFPAGTLA
Sbjct: 402 KLLALDPLNDVHVAKVNQAEAEFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLA 461

Query: 447 KPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARS 474
            P+MKD+EYIEELK+LIEKE IPAPAP+EQRWTARS
Sbjct: 462 NPSMKDLEYIEELKKLIEKEAIPAPAPIEQRWTARS 493

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NP_001292704.10.0e+0095.25L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucumis sativus] >ADT80722... [more]
KAE8637410.10.0e+0095.88hypothetical protein CSA_023387 [Cucumis sativus][more]
XP_038882838.10.0e+0095.08L-galactono-1,4-lactone dehydrogenase, mitochondrial isoform X1 [Benincasa hispi... [more]
NP_001284400.10.0e+0094.07L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucumis melo] >AAF64319.2 ... [more]
XP_023517263.10.0e+0093.73L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucurbita pepo subsp. pepo... [more]
Match NameE-valueIdentityDescription
Q9SU561.2e-26879.20L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Arabidopsis thaliana OX=... [more]
O478814.7e-26877.70L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Brassica oleracea OX=371... [more]
Q2RAP01.1e-24071.43L-galactono-1,4-lactone dehydrogenase 1, mitochondrial OS=Oryza sativa subsp. ja... [more]
Q2QXY14.2e-24071.08L-galactono-1,4-lactone dehydrogenase 2, mitochondrial OS=Oryza sativa subsp. ja... [more]
P108677.8e-3726.45L-gulonolactone oxidase OS=Rattus norvegicus OX=10116 GN=Gulo PE=1 SV=3[more]
Match NameE-valueIdentityDescription
E7E0160.0e+0095.25L-galactono-1,4-lactone dehydrogenase OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
Q9M4U90.0e+0094.07L-galactono-1,4-lactone dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103497260 PE... [more]
A0A6J1HRG80.0e+0093.22L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Cucurbita maxima OX=3661... [more]
A0A6J1EEF40.0e+0092.54L-galactono-1,4-lactone dehydrogenase, mitochondrial-like OS=Cucurbita moschata ... [more]
A0A6J1DK811.7e-30589.04L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Momordica charantia OX=3... [more]
Match NameE-valueIdentityDescription
AT3G47930.18.8e-27079.20L-galactono-1,4-lactone dehydrogenase [more]
AT3G47930.29.9e-21379.39L-galactono-1,4-lactone dehydrogenase [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 166..293
e-value: 3.5E-28
score: 99.6
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 114..244
e-value: 4.0E-26
score: 91.4
IPR010031L-gulonolactone/D-arabinono-1,4-lactone oxidasePIRSFPIRSF000136LGO_GLOcoord: 96..588
e-value: 9.0E-60
score: 200.6
IPR010031L-gulonolactone/D-arabinono-1,4-lactone oxidasePANTHERPTHR43762L-GULONOLACTONE OXIDASEcoord: 94..585
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 96..164
e-value: 3.2E-12
score: 47.6
IPR007173D-arabinono-1,4-lactone oxidasePFAMPF04030ALOcoord: 271..581
e-value: 5.8E-16
score: 59.0
IPR010029Galactonolactone dehydrogenaseTIGRFAMTIGR01676TIGR01676coord: 51..585
e-value: 0.0
score: 1210.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 27..41
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 25..47
NoneNo IPR availablePANTHERPTHR43762:SF1L-GULONOLACTONE OXIDASEcoord: 94..585
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 107..278
score: 17.234791
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 95..276

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG04G004060.1ClCG04G004060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019853 L-ascorbic acid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
cellular_component GO:0016020 membrane
molecular_function GO:0003885 D-arabinono-1,4-lactone oxidase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0016633 galactonolactone dehydrogenase activity
molecular_function GO:0080049 L-gulono-1,4-lactone dehydrogenase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016899 oxidoreductase activity, acting on the CH-OH group of donors, oxygen as acceptor