Chy9G169580 (gene) Cucumber (hystrix) v1

Overview
NameChy9G169580
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionE2 ubiquitin-conjugating enzyme
LocationchrH09: 14474556 .. 14476251 (-)
RNA-Seq ExpressionChy9G169580
SyntenyChy9G169580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCTCCAAGCAAGCGACGTGAAATGGATTTGATGAAGCTGTAAGCTTTTTTTTTTGTTTTTTTTTTTCTTTTCTTTTCCCAATTTCTTATGCTTTTCGATTGGATTCTATGTTTTTTTTTTTCATCCTTCTCCTCTTTAGGTTCTACCCTTTTACCCTTTTCTTCTTTGACTGAGCTGATGTTGATTCAGGATGATGAGTGATTACAAGGTGGAAACAATCAATGATAGCATGCAAGAGTTTTATGTGGACTTCCACGGACCTAAAGACAGTAAATCATCTATACCCATCTTTGATTTTTCCCTTTTTTGTGATCGTTTTTCTTGTGGATTAACTTCTCATATCTTACTTGGGTTTTGTTGTTGATTCTGTTGATTCTGTCTCTTTAGGATTTTCCCCCTTTTTGTTGATTCATTGTATTGAGACACGCTTTGATACTTTGCCTTTGTTTGAATCAACCAAACCTGCAGTTTGGGTCTTTATTTTCTGAGCTTGTTGTGTGGTTGACTAGTTTCAAATTGTAGTTTTGTTCTTCTTAACCTACAGTTTTGATGGTGCTGACTTTCCCATTTGAAATTAAGCCAGTTCAAGTACATTTCTAGGATTAATTAAAGGCTTTGTGATCTTATACCATTTTTCTTTTTTGCTTGTTTTGGTTGAAACTTGAAGGTCTTTACCAAGGCGGTGTGTGGAGGATTAGGGTTGAGTTGCCAGATGCTTATCCTTACAAGTCACCTTCAATTGGCTTTGTCAACAAAATTTATCATCCAAATGTTGATGAAATGTGAGTTGGCCCGATTTCGACATCGACACTTATTACATTGGTTCTCTTTATGGATAGGGCCTTATCTTCTTATGGAGTAGATTGTTGAGATTGATATATATGTACATAAATTGCAGGTCGGGCTCAGTTTGTTTGGATGTTATCAACCAGACATGGAGCCCTATGTTTGGTAAGAAACGTCTTTGTGGAATTGTACTGTTATAAGTTCGGTTGTACTTATTATGGTGATCAATTGGTTTGTTTTTAATGGAACTATAACCATTGTGGTCTAATTCGGGTATTTTTTGTTGTACAGACCTTGTAAATGTTTTCGAAGTGTTTCTTCCACAACTTCTCCTGTATCCCAACCCTTCGGATCCGTTGAACGGAGAAGCTGCTGCAGTAATGATGCGCGACCGCACTGCATATGAGCAAAGAGTAAAAGGTACTTTTATTGAATATTTAGTAGTTTGAGCGTATTGTAAGCTGTTGATTCAGTCGGTAGTATGAAACTAGATATAGACAATTTTCTTATCGCAAACTCAATTCTTGGTTGTTGATGTTGAGCTGATTCACTTGATATAGACTTACTAGCCCTGAGCTTCCTCTATAAAGAGATTCATATTTGTGTTCTGGATCTGTGACGATGATTATGAACATCAATCTCTCACGTACATCCATCGGTTATAAATTTAGCAGTCACATCCCACTCCCCACCCGACACACATCGTTCAAGTCCTGTTAGAATCCCCTGTCTCGTCGGTTTCATCTTTTACACTTAAGCTGTGTTATGTACAGAATACTGTGAGAAATATGCAAAGCCAGAAGACATTGGTGCTGTTCCGGAGGAGAAATCTAGTGATGAGGAGCTGAGTGAAGATGATTATTCCTCAGGTGATGAGGCCATGGCAGGTCAACCTGATGCTTGA

mRNA sequence

ATGTCTTCTCCAAGCAAGCGACGTGAAATGGATTTGATGAAGCTGATGATGAGTGATTACAAGGTGGAAACAATCAATGATAGCATGCAAGAGTTTTATGTGGACTTCCACGGACCTAAAGACAGTCTTTACCAAGGCGGTGTGTGGAGGATTAGGGTTGAGTTGCCAGATGCTTATCCTTACAAGTCACCTTCAATTGGCTTTGTCAACAAAATTTATCATCCAAATGTTGATGAAATGTCGGGCTCAGTTTGTTTGGATGTTATCAACCAGACATGGAGCCCTATGTTTGACCTTGTAAATGTTTTCGAAGTGTTTCTTCCACAACTTCTCCTGTATCCCAACCCTTCGGATCCGTTGAACGGAGAAGCTGCTGCAGTAATGATGCGCGACCGCACTGCATATGAGCAAAGAGTAAAAGAATACTGTGAGAAATATGCAAAGCCAGAAGACATTGGTGCTGTTCCGGAGGAGAAATCTAGTGATGAGGAGCTGAGTGAAGATGATTATTCCTCAGGTGATGAGGCCATGGCAGGTCAACCTGATGCTTGA

Coding sequence (CDS)

ATGTCTTCTCCAAGCAAGCGACGTGAAATGGATTTGATGAAGCTGATGATGAGTGATTACAAGGTGGAAACAATCAATGATAGCATGCAAGAGTTTTATGTGGACTTCCACGGACCTAAAGACAGTCTTTACCAAGGCGGTGTGTGGAGGATTAGGGTTGAGTTGCCAGATGCTTATCCTTACAAGTCACCTTCAATTGGCTTTGTCAACAAAATTTATCATCCAAATGTTGATGAAATGTCGGGCTCAGTTTGTTTGGATGTTATCAACCAGACATGGAGCCCTATGTTTGACCTTGTAAATGTTTTCGAAGTGTTTCTTCCACAACTTCTCCTGTATCCCAACCCTTCGGATCCGTTGAACGGAGAAGCTGCTGCAGTAATGATGCGCGACCGCACTGCATATGAGCAAAGAGTAAAAGAATACTGTGAGAAATATGCAAAGCCAGAAGACATTGGTGCTGTTCCGGAGGAGAAATCTAGTGATGAGGAGCTGAGTGAAGATGATTATTCCTCAGGTGATGAGGCCATGGCAGGTCAACCTGATGCTTGA

Protein sequence

MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYPYKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPLNGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQPDA*
Homology
[Close]
BLAST of Chy9G169580 vs. ExPASy Swiss-Prot
Match: P42748 (Ubiquitin-conjugating enzyme E2 4 OS=Arabidopsis thaliana OX=3702 GN=UBC4 PE=2 SV=2)

HSP 1 Score: 315.5 bits (807), Expect = 4.1e-85
Identity = 156/189 (82.54%), Postives = 170/189 (89.95%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           MSSPSKRREMD+MKLMMSDYKVETIND MQEFYV+F+GPKDSLYQGGVW+IRVELPDAYP
Sbjct: 1   MSSPSKRREMDMMKLMMSDYKVETINDGMQEFYVEFNGPKDSLYQGGVWKIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPS+GF+ KIYHPNVDE+SGSVCLDVINQTWSPMFDLVNVFE FLPQLLLYPNPSDPL
Sbjct: 61  YKSPSVGFITKIYHPNVDELSGSVCLDVINQTWSPMFDLVNVFETFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDE----- 180
           NGEAAA+MMRDR AYEQRVKEYCEKYAKP   G   E+KSSDEELSE++Y S +E     
Sbjct: 121 NGEAAALMMRDRPAYEQRVKEYCEKYAKP---GEGSEDKSSDEELSEEEYGSDNEDDDDD 180

Query: 181 --AMAGQPD 183
             A+AG+PD
Sbjct: 181 DVAIAGKPD 186

[Close]
BLAST of Chy9G169580 vs. ExPASy Swiss-Prot
Match: P16577 (Ubiquitin-conjugating enzyme E2-23 kDa OS=Triticum aestivum OX=4565 GN=UBC4 PE=1 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 5.0e-83
Identity = 148/183 (80.87%), Postives = 164/183 (89.62%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKV+ IND M EF+V FHGPKDS+YQGGVW++RVEL +AYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVDMINDGMHEFFVHFHGPKDSIYQGGVWKVRVELTEAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGF NKIYHPNVDEMSGSVCLDVINQTWSPMFDLVN+FEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFTNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNIFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSE-DDYSSGDEAMAG 180
           NGEAA++MMRD+ AYE +VKEYCE+YAKPEDI    EE+ SDEELS+ + Y SGDEA+ G
Sbjct: 121 NGEAASLMMRDKNAYENKVKEYCERYAKPEDISPEEEEEESDEELSDAEGYDSGDEAIMG 180

Query: 181 QPD 183
             D
Sbjct: 181 HAD 183

[Close]
BLAST of Chy9G169580 vs. ExPASy Swiss-Prot
Match: P42749 (Ubiquitin-conjugating enzyme E2 5 OS=Arabidopsis thaliana OX=3702 GN=UBC5 PE=2 SV=2)

HSP 1 Score: 303.5 bits (776), Expect = 1.6e-81
Identity = 151/187 (80.75%), Postives = 165/187 (88.24%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVE IND MQEF+V+F GPKDS+Y+GGVW+IRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDGMQEFFVEFSGPKDSIYEGGVWKIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPS+GF+ KIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFE FLPQLLLYPNPSDPL
Sbjct: 61  YKSPSVGFITKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFETFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSS-----GDE 180
           NGEAAA+MMRDR  YEQRVKEYCEKYAKP    A  EE SSD+E+SED+Y+S      D 
Sbjct: 121 NGEAAALMMRDRPTYEQRVKEYCEKYAKPR---ADTEEMSSDDEMSEDEYASDGDDEDDV 180

Query: 181 AMAGQPD 183
           A+AG+ D
Sbjct: 181 AIAGKLD 184

[Close]
BLAST of Chy9G169580 vs. ExPASy Swiss-Prot
Match: P42750 (Ubiquitin-conjugating enzyme E2 6 OS=Arabidopsis thaliana OX=3702 GN=UBC6 PE=2 SV=2)

HSP 1 Score: 282.7 bits (722), Expect = 2.9e-75
Identity = 133/182 (73.08%), Postives = 155/182 (85.16%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           M+SPSKRREMD+MKLMMSDYKV+T+ND +Q FYV FHGP DSLYQGGVW+I+VELP+AYP
Sbjct: 1   MASPSKRREMDMMKLMMSDYKVDTVNDDLQMFYVTFHGPTDSLYQGGVWKIKVELPEAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPS+GFVNKIYHPNVDE SG+VCLDVINQTWSPMFDL+NVFE FLPQLLLYPNPSDP 
Sbjct: 61  YKSPSVGFVNKIYHPNVDESSGAVCLDVINQTWSPMFDLINVFESFLPQLLLYPNPSDPF 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180
           NGEAA+++MRDR AYE +VKEYCEKYAKPE+I +  ++  S  E   D     D+ + G+
Sbjct: 121 NGEAASLLMRDRAAYELKVKEYCEKYAKPEEILSDDDDDDSMSEDGSDSDDDDDDEIVGK 180

Query: 181 PD 183
            D
Sbjct: 181 AD 182

[Close]
BLAST of Chy9G169580 vs. ExPASy Swiss-Prot
Match: Q9P7R4 (Ubiquitin-conjugating enzyme E2 8 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ubc8 PE=3 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.3e-62
Identity = 112/183 (61.20%), Postives = 144/183 (78.69%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           MSSP +R E D+MKL+MSDY+V  +ND+MQEFYV FHGP ++ Y GG+W++ VELP  YP
Sbjct: 1   MSSPRRRIETDVMKLLMSDYEVTLVNDNMQEFYVRFHGPSETPYSGGIWKVHVELPSEYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           +KSPSIGFVN+I+HPN+DE+SGSVCLDVINQTWSPMFD++N+FEVFLPQLL YPN SDPL
Sbjct: 61  WKSPSIGFVNRIFHPNIDELSGSVCLDVINQTWSPMFDMINIFEVFLPQLLRYPNASDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180
           NGEAAA+++R+   Y  +V++Y  +YA  ED      + S D+ +S  D  SGDE +AG 
Sbjct: 121 NGEAAALLLREPNTYYAKVRDYIARYANKEDADITLNDSSDDDTMSSIDTESGDE-IAGP 180

Query: 181 PDA 184
            D+
Sbjct: 181 MDS 182

[Close]
BLAST of Chy9G169580 vs. ExPASy TrEMBL
Match: A0A5A7THI1 (Ubiquitin-conjugating enzyme E2 4-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002420 PE=3 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 6.7e-99
Identity = 182/183 (99.45%), Postives = 182/183 (99.45%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180

Query: 181 PDA 184
            DA
Sbjct: 181 ADA 183

[Close]
BLAST of Chy9G169580 vs. ExPASy TrEMBL
Match: A0A0A0KNV9 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152970 PE=3 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 6.7e-99
Identity = 182/183 (99.45%), Postives = 182/183 (99.45%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180

Query: 181 PDA 184
            DA
Sbjct: 181 ADA 183

[Close]
BLAST of Chy9G169580 vs. ExPASy TrEMBL
Match: A0A1S3AUA7 (ubiquitin-conjugating enzyme E2 4-like OS=Cucumis melo OX=3656 GN=LOC103482765 PE=3 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 6.7e-99
Identity = 182/183 (99.45%), Postives = 182/183 (99.45%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180

Query: 181 PDA 184
            DA
Sbjct: 181 ADA 183

[Close]
BLAST of Chy9G169580 vs. ExPASy TrEMBL
Match: A0A6J1E0W4 (ubiquitin-conjugating enzyme E2 4-like OS=Momordica charantia OX=3673 GN=LOC111026131 PE=3 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.8e-96
Identity = 178/183 (97.27%), Postives = 179/183 (97.81%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVE INDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDY+S DE MAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYASSDEDMAGQ 180

Query: 181 PDA 184
            DA
Sbjct: 181 ADA 183

[Close]
BLAST of Chy9G169580 vs. ExPASy TrEMBL
Match: A0A6J1HY74 (ubiquitin-conjugating enzyme E2 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469098 PE=3 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 3.5e-95
Identity = 174/182 (95.60%), Postives = 178/182 (97.80%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLY+GGVWRI+VELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYEGGVWRIKVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVF+VFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFDVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKE+CEKYAKPEDIGA PEEKSSDEELSEDDYSS DE MAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEFCEKYAKPEDIGAAPEEKSSDEELSEDDYSSNDEGMAGQ 180

Query: 181 PD 183
            D
Sbjct: 181 AD 182

[Close]
BLAST of Chy9G169580 vs. NCBI nr
Match: XP_004143895.1 (ubiquitin-conjugating enzyme E2 4 [Cucumis sativus] >XP_008437307.1 PREDICTED: ubiquitin-conjugating enzyme E2 4-like [Cucumis melo] >XP_038906796.1 ubiquitin-conjugating enzyme E2 4-like [Benincasa hispida] >KAA0042743.1 ubiquitin-conjugating enzyme E2 4-like isoform X1 [Cucumis melo var. makuwa] >KGN50082.1 hypothetical protein Csa_000238 [Cucumis sativus])

HSP 1 Score: 370 bits (950), Expect = 2.88e-129
Identity = 182/183 (99.45%), Postives = 182/183 (99.45%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180

Query: 181 PDA 183
            DA
Sbjct: 181 ADA 183

[Close]
BLAST of Chy9G169580 vs. NCBI nr
Match: XP_022159827.1 (ubiquitin-conjugating enzyme E2 4-like [Momordica charantia])

HSP 1 Score: 362 bits (929), Expect = 4.59e-126
Identity = 178/183 (97.27%), Postives = 179/183 (97.81%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVE INDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDY+S DE MAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYASSDEDMAGQ 180

Query: 181 PDA 183
            DA
Sbjct: 181 ADA 183

[Close]
BLAST of Chy9G169580 vs. NCBI nr
Match: XP_022929074.1 (ubiquitin-conjugating enzyme E2 4-like [Cucurbita moschata] >XP_022970107.1 ubiquitin-conjugating enzyme E2 4-like [Cucurbita maxima] >XP_023549616.1 ubiquitin-conjugating enzyme E2 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 358 bits (919), Expect = 1.54e-124
Identity = 174/182 (95.60%), Postives = 178/182 (97.80%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLY+GGVWRI+VELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYEGGVWRIKVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVF+VFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFDVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKE+CEKYAKPEDIGA PEEKSSDEELSEDDYSS DE MAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEFCEKYAKPEDIGAAPEEKSSDEELSEDDYSSNDEGMAGQ 180

Query: 181 PD 182
            D
Sbjct: 181 AD 182

[Close]
BLAST of Chy9G169580 vs. NCBI nr
Match: KAG6579517.1 (Ubiquitin-conjugating enzyme E2 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 357 bits (917), Expect = 3.10e-124
Identity = 173/182 (95.05%), Postives = 178/182 (97.80%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLY+GGVWRI+VELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYEGGVWRIKVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVF+VFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFDVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKE+CEKYAKPEDIGA PEEKSSDEE+SEDDYSS DE MAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEFCEKYAKPEDIGAAPEEKSSDEEMSEDDYSSNDEGMAGQ 180

Query: 181 PD 182
            D
Sbjct: 181 AD 182

[Close]
BLAST of Chy9G169580 vs. NCBI nr
Match: XP_022996146.1 (ubiquitin-conjugating enzyme E2 4-like [Cucurbita maxima])

HSP 1 Score: 357 bits (917), Expect = 3.10e-124
Identity = 175/183 (95.63%), Postives = 178/183 (97.27%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGF+NKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFINKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIG V EEKSSDEE+SED+YSS DE MAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGGVGEEKSSDEEVSEDEYSSDDEEMAGQ 180

Query: 181 PDA 183
            DA
Sbjct: 181 ADA 183

[Close]
BLAST of Chy9G169580 vs. TAIR 10
Match: AT5G41340.1 (ubiquitin conjugating enzyme 4 )

HSP 1 Score: 315.5 bits (807), Expect = 2.9e-86
Identity = 156/189 (82.54%), Postives = 170/189 (89.95%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           MSSPSKRREMD+MKLMMSDYKVETIND MQEFYV+F+GPKDSLYQGGVW+IRVELPDAYP
Sbjct: 1   MSSPSKRREMDMMKLMMSDYKVETINDGMQEFYVEFNGPKDSLYQGGVWKIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPS+GF+ KIYHPNVDE+SGSVCLDVINQTWSPMFDLVNVFE FLPQLLLYPNPSDPL
Sbjct: 61  YKSPSVGFITKIYHPNVDELSGSVCLDVINQTWSPMFDLVNVFETFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDE----- 180
           NGEAAA+MMRDR AYEQRVKEYCEKYAKP   G   E+KSSDEELSE++Y S +E     
Sbjct: 121 NGEAAALMMRDRPAYEQRVKEYCEKYAKP---GEGSEDKSSDEELSEEEYGSDNEDDDDD 180

Query: 181 --AMAGQPD 183
             A+AG+PD
Sbjct: 181 DVAIAGKPD 186

[Close]
BLAST of Chy9G169580 vs. TAIR 10
Match: AT1G63800.1 (ubiquitin-conjugating enzyme 5 )

HSP 1 Score: 303.5 bits (776), Expect = 1.1e-82
Identity = 151/187 (80.75%), Postives = 165/187 (88.24%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVE IND MQEF+V+F GPKDS+Y+GGVW+IRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDGMQEFFVEFSGPKDSIYEGGVWKIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPS+GF+ KIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFE FLPQLLLYPNPSDPL
Sbjct: 61  YKSPSVGFITKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFETFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSS-----GDE 180
           NGEAAA+MMRDR  YEQRVKEYCEKYAKP    A  EE SSD+E+SED+Y+S      D 
Sbjct: 121 NGEAAALMMRDRPTYEQRVKEYCEKYAKPR---ADTEEMSSDDEMSEDEYASDGDDEDDV 180

Query: 181 AMAGQPD 183
           A+AG+ D
Sbjct: 181 AIAGKLD 184

[Close]
BLAST of Chy9G169580 vs. TAIR 10
Match: AT2G46030.1 (ubiquitin-conjugating enzyme 6 )

HSP 1 Score: 282.7 bits (722), Expect = 2.1e-76
Identity = 133/182 (73.08%), Postives = 155/182 (85.16%), Query Frame = 0 

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60
           M+SPSKRREMD+MKLMMSDYKV+T+ND +Q FYV FHGP DSLYQGGVW+I+VELP+AYP
Sbjct: 1   MASPSKRREMDMMKLMMSDYKVDTVNDDLQMFYVTFHGPTDSLYQGGVWKIKVELPEAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPS+GFVNKIYHPNVDE SG+VCLDVINQTWSPMFDL+NVFE FLPQLLLYPNPSDP 
Sbjct: 61  YKSPSVGFVNKIYHPNVDESSGAVCLDVINQTWSPMFDLINVFESFLPQLLLYPNPSDPF 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180
           NGEAA+++MRDR AYE +VKEYCEKYAKPE+I +  ++  S  E   D     D+ + G+
Sbjct: 121 NGEAASLLMRDRAAYELKVKEYCEKYAKPEEILSDDDDDDSMSEDGSDSDDDDDDEIVGK 180

Query: 181 PD 183
            D
Sbjct: 181 AD 182

[Close]
BLAST of Chy9G169580 vs. TAIR 10
Match: AT3G08690.1 (ubiquitin-conjugating enzyme 11 )

HSP 1 Score: 101.3 bits (251), Expect = 8.6e-22
Identity = 49/137 (35.77%), Postives = 75/137 (54.74%), Query Frame = 0 

Query: 11  DLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYPYKSPSIGFVN 70
           DL K   S+     + + M  +     GP +S Y GGV+ + +  P  YP+K P + F  
Sbjct: 12  DLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYPFKPPKVSFKT 71

Query: 71  KIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPLNGEAAAVMMR 130
           K+YHPN++  +GS+CLD++ + WSP   +  V  + +  LL  PNP DPL  E A +   
Sbjct: 72  KVYHPNINS-NGSICLDILKEQWSPALTISKVL-LSICSLLTDPNPDDPLVPEIAHMYKT 131

Query: 131 DRTAYEQRVKEYCEKYA 148
           DR+ YE   + + +KYA
Sbjct: 132 DRSKYESTARSWTQKYA 146

[Close]
BLAST of Chy9G169580 vs. TAIR 10
Match: AT3G08690.2 (ubiquitin-conjugating enzyme 11 )

HSP 1 Score: 101.3 bits (251), Expect = 8.6e-22
Identity = 49/137 (35.77%), Postives = 75/137 (54.74%), Query Frame = 0 

Query: 11  DLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYPYKSPSIGFVN 70
           DL K   S+     + + M  +     GP +S Y GGV+ + +  P  YP+K P + F  
Sbjct: 12  DLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYPFKPPKVSFKT 71

Query: 71  KIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPLNGEAAAVMMR 130
           K+YHPN++  +GS+CLD++ + WSP   +  V  + +  LL  PNP DPL  E A +   
Sbjct: 72  KVYHPNINS-NGSICLDILKEQWSPALTISKVL-LSICSLLTDPNPDDPLVPEIAHMYKT 131

Query: 131 DRTAYEQRVKEYCEKYA 148
           DR+ YE   + + +KYA
Sbjct: 132 DRSKYESTARSWTQKYA 146

The following BLAST results are available for this feature:
BLAST of Chy9G169580 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P427484.1e-8582.54Ubiquitin-conjugating enzyme E2 4 OS=Arabidopsis thaliana OX=3702 GN=UBC4 PE=2 S... [more]
P165775.0e-8380.87Ubiquitin-conjugating enzyme E2-23 kDa OS=Triticum aestivum OX=4565 GN=UBC4 PE=1... [more]
P427491.6e-8180.75Ubiquitin-conjugating enzyme E2 5 OS=Arabidopsis thaliana OX=3702 GN=UBC5 PE=2 S... [more]
P427502.9e-7573.08Ubiquitin-conjugating enzyme E2 6 OS=Arabidopsis thaliana OX=3702 GN=UBC6 PE=2 S... [more]
Q9P7R41.3e-6261.20Ubiquitin-conjugating enzyme E2 8 OS=Schizosaccharomyces pombe (strain 972 / ATC... [more]
BLAST of Chy9G169580 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7THI16.7e-9999.45Ubiquitin-conjugating enzyme E2 4-like isoform X1 OS=Cucumis melo var. makuwa OX... [more]
A0A0A0KNV96.7e-9999.45UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152970 PE... [more]
A0A1S3AUA76.7e-9999.45ubiquitin-conjugating enzyme E2 4-like OS=Cucumis melo OX=3656 GN=LOC103482765 P... [more]
A0A6J1E0W41.8e-9697.27ubiquitin-conjugating enzyme E2 4-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1HY743.5e-9595.60ubiquitin-conjugating enzyme E2 4-like OS=Cucurbita maxima OX=3661 GN=LOC1114690... [more]
BLAST of Chy9G169580 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004143895.12.88e-12999.45ubiquitin-conjugating enzyme E2 4 [Cucumis sativus] >XP_008437307.1 PREDICTED: u... [more]
XP_022159827.14.59e-12697.27ubiquitin-conjugating enzyme E2 4-like [Momordica charantia][more]
XP_022929074.11.54e-12495.60ubiquitin-conjugating enzyme E2 4-like [Cucurbita moschata] >XP_022970107.1 ubiq... [more]
KAG6579517.13.10e-12495.05Ubiquitin-conjugating enzyme E2 5, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022996146.13.10e-12495.63ubiquitin-conjugating enzyme E2 4-like [Cucurbita maxima][more]
BLAST of Chy9G169580 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G41340.12.9e-8682.54ubiquitin conjugating enzyme 4 [more]
AT1G63800.11.1e-8280.75ubiquitin-conjugating enzyme 5 [more]
AT2G46030.12.1e-7673.08ubiquitin-conjugating enzyme 6 [more]
AT3G08690.18.6e-2235.77ubiquitin-conjugating enzyme 11 [more]
AT3G08690.28.6e-2235.77ubiquitin-conjugating enzyme 11 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 4..148
e-value: 2.1E-50
score: 183.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 150..183
NoneNo IPR availablePANTHERPTHR24068:SF294UBIQUITIN-CONJUGATING ENZYME E2-21 KDA 1coord: 3..182
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 3..182
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 10..142
e-value: 4.4E-39
score: 133.3
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 1..148
score: 42.400307
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 7..143
e-value: 8.79917E-51
score: 158.134
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 1..159
e-value: 2.8E-50
score: 172.0
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 4..148
IPR023313Ubiquitin-conjugating enzyme, active sitePROSITEPS00183UBC_1coord: 73..89

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy9G169580.1Chy9G169580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0061631 ubiquitin conjugating enzyme activity