Chy9G166230 (gene) Cucumber (hystrix) v1

Overview
NameChy9G166230
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionEukaryotic aspartyl protease family protein
LocationchrH09: 11865813 .. 11867177 (-)
RNA-Seq ExpressionChy9G166230
SyntenyChy9G166230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAGTCCTCTCCATTTCCCCATTCTTCCTTCTCATTCTTCTCTTCTCCTTCTTTCTCACACATCTCCCCAACCCCAATGCCACCGCCGACTTCCTGAAGCTTCCCCTTCTTCACAAACCCCCTTTCTCCTCCCCTTCCCAATCCCTCTCCTCCGACACCCACCGCCTCTCCCTACTCTTCTCTCGCCCCAACCCCACTCTTAAATCCCCTCTCATCTCCGGCGCTTCCACCGGCTCCGGCCAATACTTCGTCGACATCCGCCTCGGTACCCCTCCCCAAAGTCTCCTTCTCGTCGCCGATACCGGCAGCGACCTCGTCTGGGTCAAATGCTCCGCCTGCCGCAACTGCTCTCACCACCCTCCTTCCTCCGCCTTCCTCCCCCGCCATTCCTCCTCCTTCTCCCCTTTCCACTGCTTCGACCCCCACTGCCGTCTCCTCCCCCACGCTCCTCACCATCTCTGTAACCACACGCGCCTCCACTCCCCTTGTCGCTTCCTCTACTCCTATGCCGATGGCTCCCTCTCCTCCGGCTTCTTCTCCAAAGAAACCACCACATTGAAGTCGCTCTCCGGGTCCGAAATCCATCTTAAAGGCCTCTCGTTCGGCTGCGGATTTCGGATCTCCGGTCCCAGCGTTTCGGGGGCTCAGTTCAATGGTGCACGTGGCGTCATGGGATTGGGTAGAGGCTCCATTTCCTTCTCTTCTCAACTCGGCCGCCGATTCGGCAACAAATTTTCTTACTGTCTTATGGATTACACTCTCTCTCCGCCGCCCACCAGCTTCTTAATGATCGGCGGCGGCCTCCACAGCCTCCCTCTCACCAATGCCACAAAAATCAGCTACACCCCTTTGCAGATTAACCCTCTTTCCCCCACATTCTACTACATTACCATCCACAGCATCACCATCGACGGCGTGAAATTACCCATCAACCCCGCCGTTTGGGAAATCGACGAACAGGGCAACGGCGGCACGGTGGTGGATTCAGGGACAACGCTAACCTACCTAACGAAGACAGCGTACGAGGAGGTGCTGAAGTCAGTAAGACGGCGAGTGAAACTACCAAATGCGGCGGAGTTGACACCGGGGTTCGATCTATGCGTGAACGCGTCGGGAGAGTCGCGGCTGCCGAGTCTGCCGCGACTGAGATTCCGACTGGGAGGTGGGGCGGTGTTTGCTCCGCCGCCGAGGAACTATTTTCTGGAAACGGAGGAGGGAGTGATGTGTTTGGCGATCCGAGCGGTGGCATCGGGAAATGGGTTTTCGGTGATCGGAAATCTGATGCAGCAAGGATTCTTGTTGGAGTTCGATAAGGAGGAATCGAGGCTGGGTTTTACAAGGCGGGGCTGTGGGCTTCCATGA

mRNA sequence

ATGCCAGTCCTCTCCATTTCCCCATTCTTCCTTCTCATTCTTCTCTTCTCCTTCTTTCTCACACATCTCCCCAACCCCAATGCCACCGCCGACTTCCTGAAGCTTCCCCTTCTTCACAAACCCCCTTTCTCCTCCCCTTCCCAATCCCTCTCCTCCGACACCCACCGCCTCTCCCTACTCTTCTCTCGCCCCAACCCCACTCTTAAATCCCCTCTCATCTCCGGCGCTTCCACCGGCTCCGGCCAATACTTCGTCGACATCCGCCTCGGTACCCCTCCCCAAAGTCTCCTTCTCGTCGCCGATACCGGCAGCGACCTCGTCTGGGTCAAATGCTCCGCCTGCCGCAACTGCTCTCACCACCCTCCTTCCTCCGCCTTCCTCCCCCGCCATTCCTCCTCCTTCTCCCCTTTCCACTGCTTCGACCCCCACTGCCGTCTCCTCCCCCACGCTCCTCACCATCTCTGTAACCACACGCGCCTCCACTCCCCTTGTCGCTTCCTCTACTCCTATGCCGATGGCTCCCTCTCCTCCGGCTTCTTCTCCAAAGAAACCACCACATTGAAGTCGCTCTCCGGGTCCGAAATCCATCTTAAAGGCCTCTCGTTCGGCTGCGGATTTCGGATCTCCGGTCCCAGCGTTTCGGGGGCTCAGTTCAATGGTGCACGTGGCGTCATGGGATTGGGTAGAGGCTCCATTTCCTTCTCTTCTCAACTCGGCCGCCGATTCGGCAACAAATTTTCTTACTGTCTTATGGATTACACTCTCTCTCCGCCGCCCACCAGCTTCTTAATGATCGGCGGCGGCCTCCACAGCCTCCCTCTCACCAATGCCACAAAAATCAGCTACACCCCTTTGCAGATTAACCCTCTTTCCCCCACATTCTACTACATTACCATCCACAGCATCACCATCGACGGCGTGAAATTACCCATCAACCCCGCCGTTTGGGAAATCGACGAACAGGGCAACGGCGGCACGGTGGTGGATTCAGGGACAACGCTAACCTACCTAACGAAGACAGCGTACGAGGAGGTGCTGAAGTCAGTAAGACGGCGAGTGAAACTACCAAATGCGGCGGAGTTGACACCGGGGTTCGATCTATGCGTGAACGCGTCGGGAGAGTCGCGGCTGCCGAGTCTGCCGCGACTGAGATTCCGACTGGGAGGTGGGGCGGTGTTTGCTCCGCCGCCGAGGAACTATTTTCTGGAAACGGAGGAGGGAGTGATGTGTTTGGCGATCCGAGCGGTGGCATCGGGAAATGGGTTTTCGGTGATCGGAAATCTGATGCAGCAAGGATTCTTGTTGGAGTTCGATAAGGAGGAATCGAGGCTGGGTTTTACAAGGCGGGGCTGTGGGCTTCCATGA

Coding sequence (CDS)

ATGCCAGTCCTCTCCATTTCCCCATTCTTCCTTCTCATTCTTCTCTTCTCCTTCTTTCTCACACATCTCCCCAACCCCAATGCCACCGCCGACTTCCTGAAGCTTCCCCTTCTTCACAAACCCCCTTTCTCCTCCCCTTCCCAATCCCTCTCCTCCGACACCCACCGCCTCTCCCTACTCTTCTCTCGCCCCAACCCCACTCTTAAATCCCCTCTCATCTCCGGCGCTTCCACCGGCTCCGGCCAATACTTCGTCGACATCCGCCTCGGTACCCCTCCCCAAAGTCTCCTTCTCGTCGCCGATACCGGCAGCGACCTCGTCTGGGTCAAATGCTCCGCCTGCCGCAACTGCTCTCACCACCCTCCTTCCTCCGCCTTCCTCCCCCGCCATTCCTCCTCCTTCTCCCCTTTCCACTGCTTCGACCCCCACTGCCGTCTCCTCCCCCACGCTCCTCACCATCTCTGTAACCACACGCGCCTCCACTCCCCTTGTCGCTTCCTCTACTCCTATGCCGATGGCTCCCTCTCCTCCGGCTTCTTCTCCAAAGAAACCACCACATTGAAGTCGCTCTCCGGGTCCGAAATCCATCTTAAAGGCCTCTCGTTCGGCTGCGGATTTCGGATCTCCGGTCCCAGCGTTTCGGGGGCTCAGTTCAATGGTGCACGTGGCGTCATGGGATTGGGTAGAGGCTCCATTTCCTTCTCTTCTCAACTCGGCCGCCGATTCGGCAACAAATTTTCTTACTGTCTTATGGATTACACTCTCTCTCCGCCGCCCACCAGCTTCTTAATGATCGGCGGCGGCCTCCACAGCCTCCCTCTCACCAATGCCACAAAAATCAGCTACACCCCTTTGCAGATTAACCCTCTTTCCCCCACATTCTACTACATTACCATCCACAGCATCACCATCGACGGCGTGAAATTACCCATCAACCCCGCCGTTTGGGAAATCGACGAACAGGGCAACGGCGGCACGGTGGTGGATTCAGGGACAACGCTAACCTACCTAACGAAGACAGCGTACGAGGAGGTGCTGAAGTCAGTAAGACGGCGAGTGAAACTACCAAATGCGGCGGAGTTGACACCGGGGTTCGATCTATGCGTGAACGCGTCGGGAGAGTCGCGGCTGCCGAGTCTGCCGCGACTGAGATTCCGACTGGGAGGTGGGGCGGTGTTTGCTCCGCCGCCGAGGAACTATTTTCTGGAAACGGAGGAGGGAGTGATGTGTTTGGCGATCCGAGCGGTGGCATCGGGAAATGGGTTTTCGGTGATCGGAAATCTGATGCAGCAAGGATTCTTGTTGGAGTTCGATAAGGAGGAATCGAGGCTGGGTTTTACAAGGCGGGGCTGTGGGCTTCCATGA

Protein sequence

MPVLSISPFFLLILLFSFFLTHLPNPNATADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAELTPGFDLCVNASGESRLPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVASGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP*
Homology
[Close]
BLAST of Chy9G166230 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.5e-59
Identity = 141/386 (36.53%), Postives = 208/386 (53.89%), Query Frame = 0 

Query: 70  SPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPR 129
           S ++SG S GSG+YF  + +GTP + + +V DTGSD+VW++C+ CR C +      F PR
Sbjct: 129 SSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC-YSQSDPIFDPR 188

Query: 130 HSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFSKETTTLKS 189
            S +++   C  PHCR L  A    CN  R    C +  SY DGS + G FS ET T + 
Sbjct: 189 KSKTYATIPCSSPHCRRLDSAG---CNTRR--KTCLYQVSYGDGSFTVGDFSTETLTFR- 248

Query: 190 LSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFGNKFSYC 249
                  +KG++ GCG    G       F GA G++GLG+G +SF  Q G RF  KFSYC
Sbjct: 249 ----RNRVKGVALGCGHDNEG------LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYC 308

Query: 250 LMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITIHSITIDGVKL 309
           L+D + S  P+S +     +  +         +TPL  NP   TFYY+ +  I++ G ++
Sbjct: 309 LVDRSASSKPSSVVFGNAAVSRI-------ARFTPLLSNPKLDTFYYVGLLGISVGGTRV 368

Query: 310 P-INPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAELTPGFDLC 369
           P +  +++++D+ GNGG ++DSGT++T L + AY  +  + R   K    A     FD C
Sbjct: 369 PGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTC 428

Query: 370 VNAS--GESRLPSLPRLRFRLGGGAVFAPPPRNYFLETE-EGVMCLAIRAVASGNGFSVI 429
            + S   E ++P++  L FR   GA  + P  NY +  +  G  C A     +  G S+I
Sbjct: 429 FDLSNMNEVKVPTVV-LHFR---GADVSLPATNYLIPVDTNGKFCFAF--AGTMGGLSII 484

Query: 430 GNLMQQGFLLEFDKEESRLGFTRRGC 452
           GN+ QQGF + +D   SR+GF   GC
Sbjct: 489 GNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]
BLAST of Chy9G166230 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 6.0e-53
Identity = 127/374 (33.96%), Postives = 195/374 (52.14%), Query Frame = 0 

Query: 79  GSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFH 138
           G G+Y +++ +GTP  S   + DTGSDL+W +C  C  C    P+  F P+ SSSFS   
Sbjct: 92  GDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQC-FSQPTPIFNPQDSSSFSTLP 151

Query: 139 CFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFSKETTTLKSLSGSEIHLK 198
           C   +C+ L   P   CN    ++ C++ Y Y DGS + G+ + ET T ++ S     + 
Sbjct: 152 CESQYCQDL---PSETCN----NNECQYTYGYGDGSTTQGYMATETFTFETSS-----VP 211

Query: 199 GLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPP 258
            ++FGCG    G      Q NGA G++G+G G +S  SQLG     +FSYC+  Y  S P
Sbjct: 212 NIAFGCGEDNQG----FGQGNGA-GLIGMGWGPLSLPSQLG---VGQFSYCMTSYGSSSP 271

Query: 259 PTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITIHSITIDGVKLPINPAVWEI 318
            T  L +G     +P  + +    T L  + L+PT+YYIT+  IT+ G  L I  + +++
Sbjct: 272 ST--LALGSAASGVPEGSPS----TTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQL 331

Query: 319 DEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAELTPGFDLCVNASGESRLP 378
            + G GG ++DSGTTLTYL + AY  V ++   ++ LP   E + G   C     +    
Sbjct: 332 QDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTV 391

Query: 379 SLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVASGNGFSVIGNLMQQGFLLEFD 438
            +P +  +  GG V     +N  +   EGV+CLA+ + +S  G S+ GN+ QQ   + +D
Sbjct: 392 QVPEISMQFDGG-VLNLGEQNILISPAEGVICLAMGS-SSQLGISIFGNIQQQETQVLYD 436

Query: 439 KEESRLGFTRRGCG 453
            +   + F    CG
Sbjct: 452 LQNLAVSFVPTQCG 436

[Close]
BLAST of Chy9G166230 vs. ExPASy Swiss-Prot
Match: Q9LTW4 (Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.1e-51
Identity = 145/431 (33.64%), Postives = 209/431 (48.49%), Query Frame = 0 

Query: 33  LKLPLLHK-----PPFSSPSQSLSSDTHRLSLLFSRPNPT--LKSPLISGASTGSGQYFV 92
           ++L L H+      P S     + +D  R SL+  + N T  +K  L SG   G+ QYF 
Sbjct: 49  VRLKLAHRDTLLPKPLSRIEDVIGADQKRHSLISRKRNSTVGVKMDLGSGIDYGTAQYFT 108

Query: 93  DIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCR 152
           +IR+GTP +   +V DTGS+L WV C              F    S SF    C    C+
Sbjct: 109 EIRVGTPAKKFRVVVDTGSELTWVNCR--YRARGKDNRRVFRADESKSFKTVGCLTQTCK 168

Query: 153 LLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFSKETTTLKSLSGSEIHLKGLSFGCG 212
           +       L       +PC + Y YADGS + G F+KET T+   +G    L G   GC 
Sbjct: 169 VDLMNLFSLTTCPTPSTPCSYDYRYADGSAAQGVFAKETITVGLTNGRMARLPGHLIGC- 228

Query: 213 FRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMI 272
                 S +G  F GA GV+GL     SF+S     +G KFSYCL+D+  +   +++L+ 
Sbjct: 229 ----SSSFTGQSFQGADGVLGLAFSDFSFTSTATSLYGAKFSYCLVDHLSNKNVSNYLIF 288

Query: 273 GGGLHSLPLTNATKISY---TPLQINPLSPTFYYITIHSITIDGVKLPINPAVWEIDEQG 332
           G        + +TK ++   TPL +  + P FY I +  I++    L I   VW  D   
Sbjct: 289 GS-------SRSTKTAFRRTTPLDLTRI-PPFYAINVIGISLGYDMLDIPSQVW--DATS 348

Query: 333 NGGTVVDSGTTLTYLTKTAYEEVLKSVRR-RVKLPNAAELTPGFDLCVNASGESRLPSLP 392
            GGT++DSGT+LT L   AY++V+  + R  V+L          + C + +    +  LP
Sbjct: 349 GGGTILDSGTSLTLLADAAYKQVVTGLARYLVELKRVKPEGVPIEYCFSFTSGFNVSKLP 408

Query: 393 RLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVASGN-GFSVIGNLMQQGFLLEFDKE 452
           +L F L GGA F P  ++Y ++   GV CL    V++G    +VIGN+MQQ +L EFD  
Sbjct: 409 QLTFHLKGGARFEPHRKSYLVDAAPGVKCLGF--VSAGTPATNVIGNIMQQNYLWEFDLM 460

[Close]
BLAST of Chy9G166230 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.9e-51
Identity = 138/410 (33.66%), Postives = 204/410 (49.76%), Query Frame = 0 

Query: 48  QSLSSDTHRLSLLFSRPNPTLKSPLISGAST----GSGQYFVDIRLGTPPQSLLLVADTG 107
           Q L     R S    R    L  P  SG  T    G G+Y +++ +GTP Q    + DTG
Sbjct: 58  QLLERAIERGSRRLQRLEAMLNGP--SGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTG 117

Query: 108 SDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSP 167
           SDL+W +C  C  C  +  +  F P+ SSSFS   C    C+        L + T  ++ 
Sbjct: 118 SDLIWTQCQPCTQC-FNQSTPIFNPQGSSSFSTLPCSSQLCQA-------LSSPTCSNNF 177

Query: 168 CRFLYSYADGSLSSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARG 227
           C++ Y Y DGS + G    ET T  S+S     +  ++FGCG    G      Q NGA G
Sbjct: 178 CQYTYGYGDGSETQGSMGTETLTFGSVS-----IPNITFGCGENNQG----FGQGNGA-G 237

Query: 228 VMGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYT 287
           ++G+GRG +S  SQL      KFSYC+     S P  S L++G   +S+    A   + T
Sbjct: 238 LVGMGRGPLSLPSQLD---VTKFSYCMTPIGSSTP--SNLLLGSLANSV---TAGSPNTT 297

Query: 288 PLQINPLSPTFYYITIHSITIDGVKLPINPAVWEID-EQGNGGTVVDSGTTLTYLTKTAY 347
            +Q + + PTFYYIT++ +++   +LPI+P+ + ++   G GG ++DSGTTLTY    AY
Sbjct: 298 LIQSSQI-PTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAY 357

Query: 348 EEVLKSVRRRVKLPNAAELTPGFDLCVNASGESRLPSLPRLRFRLGGGAVFAPPPRNYFL 407
           + V +    ++ LP     + GFDLC     +     +P       GG +   P  NYF+
Sbjct: 358 QSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDL-ELPSENYFI 417

Query: 408 ETEEGVMCLAIRAVASGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCG 453
               G++CLA+   +S  G S+ GN+ QQ  L+ +D   S + F    CG
Sbjct: 418 SPSNGLICLAMG--SSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQCG 435

[Close]
BLAST of Chy9G166230 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.9e-47
Identity = 142/468 (30.34%), Postives = 229/468 (48.93%), Query Frame = 0 

Query: 8   PFFLLILLFSFFLTHLPNPNATADFLKLPLLHKPPFSSPS---------QSLSSDTHRLS 67
           P  +   L  F  TH  + +++   L+  LLH+  F S +           +  DT R+S
Sbjct: 36  PLTVTATLPDFNNTHFSDESSSKYTLR--LLHRDRFPSVTYRNHHHRLHARMRRDTDRVS 95

Query: 68  LLFSR------PNPTLK-------SPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSD 127
            +  R      P+   +       S ++SG   GSG+YFV I +G+PP+   +V D+GSD
Sbjct: 96  AILRRISGKVIPSSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSD 155

Query: 128 LVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCR 187
           +VWV+C  C+ C +      F P  S S++   C    C  + ++  H          CR
Sbjct: 156 MVWVQCQPCKLC-YKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCH-------SGGCR 215

Query: 188 FLYSYADGSLSSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVM 247
           +   Y DGS     ++K T  L++L+ ++  ++ ++ GCG R  G       F GA G++
Sbjct: 216 YEVMYGDGS-----YTKGTLALETLTFAKTVVRNVAMGCGHRNRG------MFIGAAGLL 275

Query: 248 GLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPL 307
           G+G GS+SF  QL  + G  F YCL+  +     T  L+   G  +LP+      S+ PL
Sbjct: 276 GIGGGSMSFVGQLSGQTGGAFGYCLV--SRGTDSTGSLVF--GREALPV----GASWVPL 335

Query: 308 QINPLSPTFYYITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEV 367
             NP +P+FYY+ +  + + GV++P+   V+++ E G+GG V+D+GT +T L   AY   
Sbjct: 336 VRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAF 395

Query: 368 LKSVR-RRVKLPNAAELTPGFDLCVNASGESRLPSLPRLRFRLGGGAVFAPPPRNYFLET 427
               + +   LP A+ ++  FD C + SG   +  +P + F    G V   P RN+ +  
Sbjct: 396 RDGFKSQTANLPRASGVSI-FDTCYDLSGFVSV-RVPTVSFYFTEGPVLTLPARNFLMPV 455

Query: 428 EE-GVMCLAIRAVASGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGC 452
           ++ G  C A    AS  G S+IGN+ Q+G  + FD     +GF    C
Sbjct: 456 DDSGTYCFAF--AASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

[Close]
BLAST of Chy9G166230 vs. ExPASy TrEMBL
Match: A0A0A0KNH6 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G174650 PE=3 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 5.3e-262
Identity = 452/459 (98.47%), Postives = 452/459 (98.47%), Query Frame = 0 

Query: 1   MPVLSISPFFLLILLFSFFLTHLPNPNAT-----ADFLKLPLLHKPPFSSPSQSLSSDTH 60
           MPVLSISPFFLLILLFSFFLTHLPNPNAT     ADFLKLPLLHKPPFSSPSQSLSSDTH
Sbjct: 1   MPVLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTH 60

Query: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120
           RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 180
           NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL
Sbjct: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 180

Query: 181 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240
           SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS
Sbjct: 181 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240

Query: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 300
           SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY
Sbjct: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 300

Query: 301 YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360
           YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL
Sbjct: 301 YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360

Query: 361 PNAAELTPGFDLCVNASGESRLPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420
           PNAAELTPGFDLCVNASGESR PSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA
Sbjct: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420

Query: 421 VASGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 455
           V SGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP
Sbjct: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

[Close]
BLAST of Chy9G166230 vs. ExPASy TrEMBL
Match: A0A1S3CSZ8 (aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103504612 PE=3 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 6.9e-254
Identity = 440/459 (95.86%), Postives = 444/459 (96.73%), Query Frame = 0 

Query: 1   MPVLSISPFFLLILLFSFFLTHLPNPNAT-----ADFLKLPLLHKPPFSSPSQSLSSDTH 60
           MP+LSISPFFLLI LF FFLTHL NPNAT     ADFLKLPLLHKPPFSSPSQSLSSDTH
Sbjct: 1   MPMLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTH 60

Query: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120
           RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 180
           NCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPHAP H CNHT LHSPCRFLYSYADGSL
Sbjct: 121 NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSL 180

Query: 181 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240
           SSGFFSKETTTLK+LSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS
Sbjct: 181 SSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240

Query: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 300
           SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLP+ NATKISYTPLQINPLSPTFY
Sbjct: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFY 300

Query: 301 YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360
           YITI+SITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL
Sbjct: 301 YITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360

Query: 361 PNAAELTPGFDLCVNASGESRLPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420
           PNAAELTPGFDLCVNASGESR PSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA
Sbjct: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420

Query: 421 VASGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 455
           V SGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP
Sbjct: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

[Close]
BLAST of Chy9G166230 vs. ExPASy TrEMBL
Match: A0A6J1HXS2 (aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111469001 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 6.1e-226
Identity = 395/456 (86.62%), Postives = 416/456 (91.23%), Query Frame = 0 

Query: 3   VLSISPFFLLILLFSFFLTHLPNPNATADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFS 62
           +LS+S FF LILL  F L  L N  A AD+LKLPLLHK PFSSPSQ+LSSDTHRLSLLFS
Sbjct: 5   MLSVSTFFHLILLL-FSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFS 64

Query: 63  ----RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCS 122
               RPNPTLKSPLISGASTGSGQYFVD+R+GTPPQSLLLVADTGSDLVWVKCSACRNCS
Sbjct: 65  ALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCS 124

Query: 123 HHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSG 182
           HHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAP HLCNHTRLHSPCRFLY+YADGS SSG
Sbjct: 125 HHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSG 184

Query: 183 FFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQL 242
           FFSKETTTLK+LSGSE  LK LSFGCGFRISGPSVSGAQFNGARGVMGLGRG ISFS+QL
Sbjct: 185 FFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQL 244

Query: 243 GRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYIT 302
           GRRFGNKFSYCLMDYTLSPPPTS+LMIGGGL SLP+TNATKISYTPL INPLSPTFYYI 
Sbjct: 245 GRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIA 304

Query: 303 IHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNA 362
           + SIT+DGVKLPINP VW IDEQGNGGTVVDSGTTLTYL + AY+EVLK+VR+RVKLP A
Sbjct: 305 VKSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRVKLPAA 364

Query: 363 AELTPGFDLCVNASGESRLPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVAS 422
           AELTPGFDLCVN S ES+ PSLPR+RFR+G GAVFAPP RNYFLET EGVMCLAIRAV  
Sbjct: 365 AELTPGFDLCVNVSKESQRPSLPRVRFRVGNGAVFAPPARNYFLETVEGVMCLAIRAVEG 424

Query: 423 GNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 455
           GNGFSVIGNLMQQGFLLEFDKE SRLGF+RRGCGLP
Sbjct: 425 GNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]
BLAST of Chy9G166230 vs. ExPASy TrEMBL
Match: A0A6J1ELQ4 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435697 PE=3 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 1.4e-225
Identity = 392/456 (85.96%), Postives = 416/456 (91.23%), Query Frame = 0 

Query: 3   VLSISPFFLLILLFSFFLTHLPNPNATADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFS 62
           +LS+SPFF LILL  F L  L N  A AD+LKLPLLHK PFSSPSQ+LSSDTHRLSLLFS
Sbjct: 5   MLSVSPFFHLILLL-FSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFS 64

Query: 63  ----RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCS 122
               RPNPTLKSPLISGASTGSGQYFVD+R+GTPPQSLLLVADTGSDLVWVKCSACRNCS
Sbjct: 65  ALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCS 124

Query: 123 HHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSG 182
           HHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAP HLCNHTRLHSPCRFLY+YADGS SSG
Sbjct: 125 HHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSG 184

Query: 183 FFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQL 242
           FFSKETTTLK+L+GSE  LK LSFGCGFRISGPSVSGAQFNGARGVMGLGRG ISFS+QL
Sbjct: 185 FFSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQL 244

Query: 243 GRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYIT 302
           GRRFGNKFSYCLMDYTLSPPPTS+LMIGGGL  LP+TNATKISYTPL INPLSPTFYYI 
Sbjct: 245 GRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIA 304

Query: 303 IHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNA 362
           + SIT+DGVKLPINP +W IDEQGNGGTVVDSGTTLTYL + AY+EVLK++R+RVKLP A
Sbjct: 305 VKSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAA 364

Query: 363 AELTPGFDLCVNASGESRLPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVAS 422
           AELTPGFDLCVN S ES+ PSLPR+RF+LG GAVF PP RNYFLETEEGVMCLAIRAV  
Sbjct: 365 AELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEG 424

Query: 423 GNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 455
           GNGFSVIGNLMQQGFLLEFDKE SRLGF+RRGCGLP
Sbjct: 425 GNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]
BLAST of Chy9G166230 vs. ExPASy TrEMBL
Match: A0A6J1EPZ3 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436585 PE=3 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 1.3e-207
Identity = 361/453 (79.69%), Postives = 393/453 (86.75%), Query Frame = 0 

Query: 6   ISPFFLLILLFSFFLTHLPNPNATADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFS--R 65
           +  F LL+LL    L  L N    + +LK PLLH  PFSSPSQ+LSSDTHRLSLLFS  R
Sbjct: 4   VPQFLLLLLLLLSSLADLSNA-IPSQYLKFPLLHTNPFSSPSQALSSDTHRLSLLFSAHR 63

Query: 66  PNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPS 125
            +PTLKSPLISGASTGSGQYFV++ LGTPPQSLLLV DTGSDLVWVKCS CRNCSHHPPS
Sbjct: 64  HSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVVDTGSDLVWVKCSPCRNCSHHPPS 123

Query: 126 SAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFSKE 185
           SAF PRHSSSFSPFHCFDPHCRLLPH P H CNHT LHSPC FLYSYAD SLSSGFFSK+
Sbjct: 124 SAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCSFLYSYADSSLSSGFFSKD 183

Query: 186 TTTLKSLSG--SEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRR 245
            TT  + SG  ++  L  LSFGCGFRISGPSVSGA+F GARGVMGLGRG ISFSSQLG R
Sbjct: 184 VTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFTGARGVMGLGRGPISFSSQLGHR 243

Query: 246 FGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITIHS 305
           FGN FSYCLMDYTLSPPPTS+LMIGGGL SLP+TNA+KISYTPLQINPLSPTFYYI + S
Sbjct: 244 FGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKS 303

Query: 306 ITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAEL 365
           IT+DGVKLPINP VW IDEQGNGGTVVDSGTTLTYL + AYEEVLK++RRRVKLP A +L
Sbjct: 304 ITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRALQL 363

Query: 366 TPGFDLCVNASGESRLPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVASGNG 425
           +PGFDLCVNAS ESR+ SLP++RFR+GGG VFAPP RNYF+ETEEGVMCLAIR V SGNG
Sbjct: 364 SPGFDLCVNASSESRMRSLPQIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSGNG 423

Query: 426 FSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 455
           FSVIGNLMQQGFLLEFD+E+SR+GF+RRGCGLP
Sbjct: 424 FSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 455

[Close]
BLAST of Chy9G166230 vs. NCBI nr
Match: XP_004143702.1 (aspartyl protease family protein 2 [Cucumis sativus] >KGN50439.1 hypothetical protein Csa_000435 [Cucumis sativus])

HSP 1 Score: 909 bits (2348), Expect = 0.0
Identity = 452/459 (98.47%), Postives = 452/459 (98.47%), Query Frame = 0 

Query: 1   MPVLSISPFFLLILLFSFFLTHLPNPNATA-----DFLKLPLLHKPPFSSPSQSLSSDTH 60
           MPVLSISPFFLLILLFSFFLTHLPNPNATA     DFLKLPLLHKPPFSSPSQSLSSDTH
Sbjct: 1   MPVLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTH 60

Query: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120
           RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 180
           NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL
Sbjct: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 180

Query: 181 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240
           SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS
Sbjct: 181 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240

Query: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 300
           SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY
Sbjct: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 300

Query: 301 YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360
           YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL
Sbjct: 301 YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360

Query: 361 PNAAELTPGFDLCVNASGESRLPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420
           PNAAELTPGFDLCVNASGESR PSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA
Sbjct: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420

Query: 421 VASGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 454
           V SGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP
Sbjct: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

[Close]
BLAST of Chy9G166230 vs. NCBI nr
Match: XP_008467208.1 (PREDICTED: aspartyl protease family protein 2 [Cucumis melo])

HSP 1 Score: 882 bits (2278), Expect = 0.0
Identity = 440/459 (95.86%), Postives = 444/459 (96.73%), Query Frame = 0 

Query: 1   MPVLSISPFFLLILLFSFFLTHLPNPNATA-----DFLKLPLLHKPPFSSPSQSLSSDTH 60
           MP+LSISPFFLLI LF FFLTHL NPNATA     DFLKLPLLHKPPFSSPSQSLSSDTH
Sbjct: 1   MPMLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTH 60

Query: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120
           RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 180
           NCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPHAP H CNHT LHSPCRFLYSYADGSL
Sbjct: 121 NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSL 180

Query: 181 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240
           SSGFFSKETTTLK+LSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS
Sbjct: 181 SSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240

Query: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 300
           SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLP+ NATKISYTPLQINPLSPTFY
Sbjct: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFY 300

Query: 301 YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360
           YITI+SITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL
Sbjct: 301 YITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360

Query: 361 PNAAELTPGFDLCVNASGESRLPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420
           PNAAELTPGFDLCVNASGESR PSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA
Sbjct: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420

Query: 421 VASGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 454
           V SGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP
Sbjct: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

[Close]
BLAST of Chy9G166230 vs. NCBI nr
Match: XP_038907006.1 (aspartyl protease family protein 2 [Benincasa hispida])

HSP 1 Score: 820 bits (2118), Expect = 2.82e-298
Identity = 409/452 (90.49%), Postives = 427/452 (94.47%), Query Frame = 0 

Query: 4   LSISPFFLLILLFSFFLTHLPNPNATA-DFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFS 63
           L ISPFF  I LF FFLT   N NATA D+LKLPLLHKPPFSSPSQ+LSSDTHRLSLLFS
Sbjct: 2   LPISPFFHFIFLF-FFLT---NFNATAADYLKLPLLHKPPFSSPSQALSSDTHRLSLLFS 61

Query: 64  RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPP 123
           RPNPTLKSPLISGASTGSGQYFVD+RLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPP
Sbjct: 62  RPNPTLKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPP 121

Query: 124 SSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFSK 183
           S+AFLPRHSSSFSPFHCFDPHCRLLPHAP HLCNHTR HSPCRFLYSYADGSLSSGFFSK
Sbjct: 122 STAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRFHSPCRFLYSYADGSLSSGFFSK 181

Query: 184 ETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRF 243
           ETTTLK+LSGSEIHLKGLSFGCGFRISGPSVSGAQF+GARGVMGLGRGSISFSSQLGRRF
Sbjct: 182 ETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFSGARGVMGLGRGSISFSSQLGRRF 241

Query: 244 GNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITIHSI 303
           GNKFSYCLMDYTLSPPPTS+LMIGGG  SLP+TNATKISYTPLQINPLSPTFYYI IHSI
Sbjct: 242 GNKFSYCLMDYTLSPPPTSYLMIGGGHRSLPVTNATKISYTPLQINPLSPTFYYIAIHSI 301

Query: 304 TIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAELT 363
           T+DGVKLPINPAVW +D+QGNGGTVVDSGTTLTYL K AY+EVLK+VRRRVKLP+A+ELT
Sbjct: 302 TVDGVKLPINPAVWAMDKQGNGGTVVDSGTTLTYLAKAAYDEVLKAVRRRVKLPSASELT 361

Query: 364 PGFDLCVNASGESRLPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVASGNGF 423
           PGFDLCVNAS  SR PSLPRLRFRLGGGAVFAPPPRNYFLETEE VMCLAIR V SGNGF
Sbjct: 362 PGFDLCVNASDSSRRPSLPRLRFRLGGGAVFAPPPRNYFLETEERVMCLAIRPVDSGNGF 421

Query: 424 SVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 454
           SVIGNLMQQGFLLEFDK+ +RLGF+RRGCGLP
Sbjct: 422 SVIGNLMQQGFLLEFDKDAARLGFSRRGCGLP 449

[Close]
BLAST of Chy9G166230 vs. NCBI nr
Match: XP_023549997.1 (aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 792 bits (2045), Expect = 5.42e-287
Identity = 395/455 (86.81%), Postives = 416/455 (91.43%), Query Frame = 0 

Query: 4   LSISPFFLLILLFSFFLTHLPNPNATADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFS- 63
           LS+SPFF  ILL  F L  L N  A AD+LKLPLLHK PFSSPSQ+LSSDTHRLSLLFS 
Sbjct: 6   LSVSPFFHFILLL-FSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 65

Query: 64  ---RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSH 123
              RPNPTLKSPLISGASTGSGQYFVD+R+GTPPQSLLLVADTGSDLVWVKCSACRNCSH
Sbjct: 66  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 125

Query: 124 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGF 183
           HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAP HLCNHTRLHSPCRFLY+YADGS SSGF
Sbjct: 126 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 185

Query: 184 FSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLG 243
           FSKETTTLK+LSGSE  LK LSFGCGFRISGPSVSGAQFNGARGVMGLGRG ISFS+QLG
Sbjct: 186 FSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 245

Query: 244 RRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITI 303
           RRFGNKFSYCLMDYTLSPPPTS+LMIGGGL SLP+TNATKISYTPL INPLSPTFYYI +
Sbjct: 246 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAV 305

Query: 304 HSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAA 363
            SIT+DGVKLPINP VW IDEQGNGGTVVDSGTTLTYL + AY+EVLK+VR+RVKLP AA
Sbjct: 306 KSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRVKLPAAA 365

Query: 364 ELTPGFDLCVNASGESRLPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVASG 423
           ELTPGFDLCVN S ES+ PSLPR+RF+LG GAVFAPP RNYFLETEEGVMCL+IRAV  G
Sbjct: 366 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLETEEGVMCLSIRAVEGG 425

Query: 424 NGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 454
           NGFSVIGNLMQQGFLLEFDKE SRLGF+RRGCGLP
Sbjct: 426 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]
BLAST of Chy9G166230 vs. NCBI nr
Match: KAG7017139.1 (Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 788 bits (2036), Expect = 1.10e-285
Identity = 393/455 (86.37%), Postives = 416/455 (91.43%), Query Frame = 0 

Query: 4   LSISPFFLLILLFSFFLTHLPNPNATADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFS- 63
           LS+SPFF  ILL  F L  L N  A AD+LKLPLLHK PFSSPSQ+LSSDTHRLSLLFS 
Sbjct: 2   LSVSPFFHFILLL-FSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 61

Query: 64  ---RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSH 123
              RPNPTLKSPLISGASTGSGQYFVD+R+GTPPQSLLLVADTGSDLVWVKCSACRNCSH
Sbjct: 62  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 121

Query: 124 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGF 183
           HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAP HLCNHTRLHSPCRFLY+YADGS SSGF
Sbjct: 122 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 181

Query: 184 FSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLG 243
           FSKETTTLK+LSGSE  LK LSFGCGFRISGPSVSGAQFNGARGVMGLGRG ISFS+QLG
Sbjct: 182 FSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 241

Query: 244 RRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITI 303
           RRFGNKFSYCLMDYTLSPPPTS+LMIGGGL SLP+TNATKISYTPL INPLSPTFYYI +
Sbjct: 242 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAV 301

Query: 304 HSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAA 363
            SIT+DGVKLPINP VW I+EQGNGGTVVDSGTTLTYL + AY+EVLK++R+RVKLP AA
Sbjct: 302 KSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA 361

Query: 364 ELTPGFDLCVNASGESRLPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVASG 423
           ELTPGFDLCVN S ES+ PSLPR+RF+LG GAVFAPP RNYFL+TEEGVMCLAIRAV  G
Sbjct: 362 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVEGG 421

Query: 424 NGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 454
           NGFSVIGNLMQQGFLLEFDKE SRLGF+RRGCGLP
Sbjct: 422 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 455

[Close]
BLAST of Chy9G166230 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 589.0 bits (1517), Expect = 3.4e-168
Identity = 295/456 (64.69%), Postives = 352/456 (77.19%), Query Frame = 0 

Query: 10  FLLILLFSFFLTHLPNPNATA-----DFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFSR- 69
           F L    S FL  LP  N  A      +LKLPLL K PF SP+Q+L+ DT RL  L  R 
Sbjct: 6   FFLCSFLSLFL--LPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFLSLRR 65

Query: 70  -PNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPP 129
            P P +KSP++SGA++GSGQYFVD+R+G PPQSLLL+ADTGSDLVWVKCSACRNCSHH P
Sbjct: 66  KPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSHHSP 125

Query: 130 SSAFLPRHSSSFSPFHCFDPHCRLLP---HAPHHLCNHTRLHSPCRFLYSYADGSLSSGF 189
           ++ F PRHSS+FSP HC+DP CRL+P    AP  +CNHTR+HS C + Y YADGSL+SG 
Sbjct: 126 ATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAP--ICNHTRIHSTCHYEYGYADGSLTSGL 185

Query: 190 FSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLG 249
           F++ETT+LK+ SG E  LK ++FGCGFRISG SVSG  FNGA GVMGLGRG ISF+SQLG
Sbjct: 186 FARETTSLKTSSGKEARLKSVAFGCGFRISGQSVSGTSFNGANGVMGLGRGPISFASQLG 245

Query: 250 RRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITI 309
           RRFGNKFSYCLMDYTLSPPPTS+L+IG G   +     +K+ +TPL  NPLSPTFYY+ +
Sbjct: 246 RRFGNKFSYCLMDYTLSPPPTSYLIIGNGGDGI-----SKLFFTPLLTNPLSPTFYYVKL 305

Query: 310 HSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAA 369
            S+ ++G KL I+P++WEID+ GNGGTVVDSGTTL +L + AY  V+ +VRRRVKLP A 
Sbjct: 306 KSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIAD 365

Query: 370 ELTPGFDLCVNASGESRLPS-LPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVAS 429
            LTPGFDLCVN SG ++    LPRL+F   GGAVF PPPRNYF+ETEE + CLAI++V  
Sbjct: 366 ALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSVDP 425

Query: 430 GNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 455
             GFSVIGNLMQQGFL EFD++ SRLGF+RRGC LP
Sbjct: 426 KVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 452

[Close]
BLAST of Chy9G166230 vs. TAIR 10
Match: AT3G25700.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 400.2 bits (1027), Expect = 2.2e-111
Identity = 226/456 (49.56%), Postives = 267/456 (58.55%), Query Frame = 0 

Query: 10  FLLILLFSFFLTHLPNPNATA-----DFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFSR- 69
           F L    S FL  LP  N  A      +LKLPLL K PF SP+Q+L+ DT RL  L  R 
Sbjct: 6   FFLCSFLSLFL--LPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFLSLRR 65

Query: 70  -PNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPP 129
            P P +KSP++SGA++GSGQYFVD+R+G PPQSLLL+ADTGSDLVWVKCSACRNCSHH P
Sbjct: 66  KPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSHHSP 125

Query: 130 SSAFLPRHSSSFSPFHCFDPHCRLLP---HAPHHLCNHTRLHSPCRFLYSYADGSLSSGF 189
           ++ F PRHSS+FSP HC+DP CRL+P    AP  +CNHTR+HS C + Y YADGSL+SG 
Sbjct: 126 ATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAP--ICNHTRIHSTCHYEYGYADGSLTSGL 185

Query: 190 FSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLG 249
           F++ETT+LK+ SG E  LK ++FGCGFRISG SVS                         
Sbjct: 186 FARETTSLKTSSGKEARLKSVAFGCGFRISGQSVS------------------------- 245

Query: 250 RRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITI 309
                                                                       
Sbjct: 246 ------------------------------------------------------------ 305

Query: 310 HSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAA 369
                                 GNGGTVVDSGTTL +L + AY  V+ +VRRRVKLP A 
Sbjct: 306 ----------------------GNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIAD 350

Query: 370 ELTPGFDLCVNASGESRLPS-LPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVAS 429
            LTPGFDLCVN SG ++    LPRL+F   GGAVF PPPRNYF+ETEE + CLAI++V  
Sbjct: 366 ALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSVDP 350

Query: 430 GNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 455
             GFSVIGNLMQQGFL EFD++ SRLGF+RRGC LP
Sbjct: 426 KVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 350

[Close]
BLAST of Chy9G166230 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 231.9 bits (590), Expect = 1.0e-60
Identity = 141/386 (36.53%), Postives = 208/386 (53.89%), Query Frame = 0 

Query: 70  SPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPR 129
           S ++SG S GSG+YF  + +GTP + + +V DTGSD+VW++C+ CR C +      F PR
Sbjct: 129 SSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC-YSQSDPIFDPR 188

Query: 130 HSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFSKETTTLKS 189
            S +++   C  PHCR L  A    CN  R    C +  SY DGS + G FS ET T + 
Sbjct: 189 KSKTYATIPCSSPHCRRLDSAG---CNTRR--KTCLYQVSYGDGSFTVGDFSTETLTFR- 248

Query: 190 LSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFGNKFSYC 249
                  +KG++ GCG    G       F GA G++GLG+G +SF  Q G RF  KFSYC
Sbjct: 249 ----RNRVKGVALGCGHDNEG------LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYC 308

Query: 250 LMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITIHSITIDGVKL 309
           L+D + S  P+S +     +  +         +TPL  NP   TFYY+ +  I++ G ++
Sbjct: 309 LVDRSASSKPSSVVFGNAAVSRI-------ARFTPLLSNPKLDTFYYVGLLGISVGGTRV 368

Query: 310 P-INPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAELTPGFDLC 369
           P +  +++++D+ GNGG ++DSGT++T L + AY  +  + R   K    A     FD C
Sbjct: 369 PGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTC 428

Query: 370 VNAS--GESRLPSLPRLRFRLGGGAVFAPPPRNYFLETE-EGVMCLAIRAVASGNGFSVI 429
            + S   E ++P++  L FR   GA  + P  NY +  +  G  C A     +  G S+I
Sbjct: 429 FDLSNMNEVKVPTVV-LHFR---GADVSLPATNYLIPVDTNGKFCFAF--AGTMGGLSII 484

Query: 430 GNLMQQGFLLEFDKEESRLGFTRRGC 452
           GN+ QQGF + +D   SR+GF   GC
Sbjct: 489 GNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]
BLAST of Chy9G166230 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 231.1 bits (588), Expect = 1.8e-60
Identity = 148/391 (37.85%), Postives = 204/391 (52.17%), Query Frame = 0 

Query: 68  LKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFL 127
           L + L SG + GSG+YF+D+ +GTPP+   L+ DTGSDL W++C  C +C  H     + 
Sbjct: 145 LIATLESGMTLGSGEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDC-FHQNGMFYD 204

Query: 128 PRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFSKETTTL 187
           P+ S+SF    C DP C L+  +P         +  C + Y Y D S ++G F+ ET T+
Sbjct: 205 PKTSASFKNITCNDPRCSLI-SSPDPPVQCESDNQSCPYFYWYGDRSNTTGDFAVETFTV 264

Query: 188 KSLS----GSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFG 247
              +     SE  +  + FGCG    G       F+GA G++GLGRG +SFSSQL   +G
Sbjct: 265 NLTTTEGGSSEYKVGNMMFGCGHWNRG------LFSGASGLLGLGRGPLSFSSQLQSLYG 324

Query: 248 NKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITIHSIT 307
           + FSYCL+D   +   +S L+ G     L  TN    S+   + N +  TFYYI I SI 
Sbjct: 325 HSFSYCLVDRNSNTNVSSKLIFGEDKDLLNHTNLNFTSFVNGKENSVE-TFYYIQIKSIL 384

Query: 308 IDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVK--LPNAAEL 367
           + G  L I    W I   G+GGT++DSGTTL+Y  + AYE +      ++K   P   + 
Sbjct: 385 VGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDF 444

Query: 368 TPGFDLCVNASG-ESRLPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVASGN 427
            P  D C N SG E     LP L      G V+  P  N F+   E ++CLAI       
Sbjct: 445 -PVLDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSEDLVCLAILGTPKST 504

Query: 428 GFSVIGNLMQQGFLLEFDKEESRLGFTRRGC 452
            FS+IGN  QQ F + +D + SRLGFT   C
Sbjct: 505 -FSIIGNYQQQNFHILYDTKRSRLGFTPTKC 524

[Close]
BLAST of Chy9G166230 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 221.5 bits (563), Expect = 1.4e-57
Identity = 138/385 (35.84%), Postives = 203/385 (52.73%), Query Frame = 0 

Query: 72  LISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHS 131
           +ISG S GSG+YF+ + +GTP  ++ +V DTGSD+VW++CS C+ C ++   + F P+ S
Sbjct: 124 VISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKAC-YNQTDAIFDPKKS 183

Query: 132 SSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFSKETTTLKSLS 191
            +F+   C    CR L  +   +   TR    C +  SY DGS + G FS ET T     
Sbjct: 184 KTFATVPCGSRLCRRLDDSSECV---TRRSKTCLYQVSYGDGSFTEGDFSTETLTFHGAR 243

Query: 192 GSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFGNKFSYCLM 251
              + L     GCG    G       F GA G++GLGRG +SF SQ   R+  KFSYCL+
Sbjct: 244 VDHVPL-----GCGHDNEG------LFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLV 303

Query: 252 DYT----LSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITIHSITIDGV 311
           D T     S PP++ +    G  ++P T+     +TPL  NP   TFYY+ +  I++ G 
Sbjct: 304 DRTSSGSSSKPPSTIVF---GNAAVPKTSV----FTPLLTNPKLDTFYYLQLLGISVGGS 363

Query: 312 KLP-INPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAELTPGFD 371
           ++P ++ + +++D  GNGG ++DSGT++T LT+ AY  +  + R        A     FD
Sbjct: 364 RVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFD 423

Query: 372 LCVNASGESRLPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVASGNGFSVIG 431
            C + SG + +  +P + F  GGG V  P          EG  C A     +    S+IG
Sbjct: 424 TCFDLSGMTTV-KVPTVVFHFGGGEVSLPASNYLIPVNTEGRFCFAF--AGTMGSLSIIG 483

Query: 432 NLMQQGFLLEFDKEESRLGFTRRGC 452
           N+ QQGF + +D   SR+GF  R C
Sbjct: 484 NIQQQGFRVAYDLVGSRVGFLSRAC 483

The following BLAST results are available for this feature:
BLAST of Chy9G166230 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LNJ31.5e-5936.53Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q766C26.0e-5333.96Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q9LTW41.1e-5133.64Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE... [more]
Q766C31.9e-5133.66Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q9LHE32.9e-4730.34Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
BLAST of Chy9G166230 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KNH65.3e-26298.47Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G17465... [more]
A0A1S3CSZ86.9e-25495.86aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103504612 PE=3 ... [more]
A0A6J1HXS26.1e-22686.62aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111469001 P... [more]
A0A6J1ELQ41.4e-22585.96aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1EPZ31.3e-20779.69aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
BLAST of Chy9G166230 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004143702.10.098.47aspartyl protease family protein 2 [Cucumis sativus] >KGN50439.1 hypothetical pr... [more]
XP_008467208.10.095.86PREDICTED: aspartyl protease family protein 2 [Cucumis melo][more]
XP_038907006.12.82e-29890.49aspartyl protease family protein 2 [Benincasa hispida][more]
XP_023549997.15.42e-28786.81aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo][more]
KAG7017139.11.10e-28586.37Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. argyro... [more]
BLAST of Chy9G166230 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G25700.13.4e-16864.69Eukaryotic aspartyl protease family protein [more]
AT3G25700.22.2e-11149.56Eukaryotic aspartyl protease family protein [more]
AT1G01300.11.0e-6036.53Eukaryotic aspartyl protease family protein [more]
AT2G42980.11.8e-6037.85Eukaryotic aspartyl protease family protein [more]
AT3G61820.11.4e-5735.84Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 89..109
score: 49.53
coord: 326..337
score: 41.24
coord: 423..438
score: 18.99
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 83..267
e-value: 2.1E-48
score: 164.9
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 62..266
e-value: 1.0E-45
score: 158.1
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 272..452
e-value: 8.6E-50
score: 171.0
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 77..451
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 294..447
e-value: 4.5E-36
score: 124.1
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 12..452
NoneNo IPR availablePANTHERPTHR47967:SF72BNAA02G27600D PROTEINcoord: 12..452
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 83..447
score: 40.933144
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 82..451
e-value: 1.8399E-84
score: 258.347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy9G166230.1Chy9G166230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005576 extracellular region
molecular_function GO:0004190 aspartic-type endopeptidase activity