Chy9G157200 (gene) Cucumber (hystrix) v1

BLAST of Chy9G157200 vs. ExPASy Swiss-Prot
Match: Q8R1G2 (Carboxymethylenebutenolidase homolog OS=Mus musculus OX=10090 GN=Cmbl PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.0e-12
Identity = 64/248 (25.81%), Postives = 113/248 (45.56%), Query Frame = 0

Query: 66  DDDDACELVNGMELSIGEQPH----HSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRD 125
           ++ + C    G +L  G   H      I AY+ ++   + G A++++ D+F +Q  + R 
Sbjct: 3   NEANPCPCDIGHKLEYGGMGHEVQVEHIKAYVTRS-PVDAGKAVIVVQDIFGWQLPNTRY 62

Query: 126 FAYRLACIGFNVLLPD-LVHEDVWEKP------PAEHCAKQEMQRIAEDIGTVTKWLTEE 185
            A  +A  G+  ++PD  V ++ W+        PA     +  +++  ++  V ++L ++
Sbjct: 63  MADMIARNGYTTIVPDFFVGQEPWDPAGDWSTFPA-WLKSRNARKVNREVDAVLRYLRQQ 122

Query: 186 FSAAGLSMKLGIIGFGYGGGRVIDVLARDEGACFLIGASIYGTQVDPSLATKVKVPVLLI 245
             A     K+GI+GF +GG  V  V+          G S+YG   D      +K P L I
Sbjct: 123 CHA----QKIGIVGFCWGGVVVHQVMTAYPD--IRAGVSVYGIIRDSEDVYNLKNPTLFI 182

Query: 246 SGDNDPLCPVSVMKDLEKGLGE----GSKLVIFGGRGHGFVHNPG---SPEEDDDAEKAF 296
             +ND + P+  +  L + L E      ++  F G+ HGFVH      SP +    E+A 
Sbjct: 183 FAENDTVIPLEQVSTLTQKLKEHCIVNYQVKTFSGQTHGFVHRKREDCSPADKPYIEEAR 242

BLAST of Chy9G157200 vs. ExPASy Swiss-Prot
Match: Q7TP52 (Carboxymethylenebutenolidase homolog OS=Rattus norvegicus OX=10116 GN=Cmbl PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 4.5e-12
Identity = 59/209 (28.23%), Postives = 99/209 (47.37%), Query Frame = 0

Query: 102 GTAILLLSDLFAFQHSSIRDFAYRLACIGFNVLLPD-LVHEDVWEKPPA-------EHCA 161
           G A++++ D+F +Q S+ R  A  +A  G+  ++PD  V ++ W+  PA       E   
Sbjct: 42  GKAVIVVQDIFGWQLSNTRYMADMIAGNGYTTIVPDFFVGQEPWD--PAGDWSTFPEWLK 101

Query: 162 KQEMQRIAEDIGTVTKWLTEEFSAAGLSMKLGIIGFGYGGGRVIDVLARDEGACFLIGAS 221
            +  ++I  ++  V ++L ++  A     K+GI+GF +GG  V  V+          G S
Sbjct: 102 SRNARKINREVDAVLRYLKQQCHA----QKIGIVGFCWGGIVVHHVMTTYPEV--RAGVS 161

Query: 222 IYGTQVDPSLATKVKVPVLLISGDNDPLCPVSVMKDLEKGLGE----GSKLVIFGGRGHG 281
           +YG   D      +K P L I  +ND + P+  +  L + L E      ++  F G+ HG
Sbjct: 162 VYGIIRDSEDVYNLKNPTLFIFAENDAVIPLEQVSILIQKLKEHCIVNYQVKTFSGQTHG 221

Query: 282 FVHNPG---SPEEDDDAEKAFLMLRNWLH 296
           FVH      SP +    E+A   L  WL+
Sbjct: 222 FVHRKREDCSPADKPYIEEARRNLIEWLN 242

BLAST of Chy9G157200 vs. ExPASy Swiss-Prot
Match: P71505 (Putative carboxymethylenebutenolidase OS=Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) OX=272630 GN=MexAM1_META1p1735 PE=3 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 6.4e-06
Identity = 57/232 (24.57%), Postives = 94/232 (40.52%), Query Frame = 0

Query: 89  IPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDFAYRLACIGFNVLLPDLV--HEDV--- 148
           IPAY     +      IL++ ++F   H  I+D   RLA +G+  L P+L     DV   
Sbjct: 65  IPAYRAMPAEGGPFPTILVVQEIFGV-HEHIKDVCRRLAKLGYFALAPELYARQGDVSTL 124

Query: 149 --WEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAGLSMKLGIIGFGYGGGRVIDVLA 208
              ++  +E  +K    ++  D+     +      A   + +LGI GF +GG   I  L 
Sbjct: 125 TNIQQIVSEVVSKVPDAQVMSDLDAAVAFAKGTGKAD--TARLGITGFCWGGR--ITWLY 184

Query: 209 RDEGACFLIGASIYGTQVDPS----------LATKVKVPVLLISGDND---PLCPVSVMK 268
                    G + YG  V  S          +A  +K PVL + G  D   P+  +  MK
Sbjct: 185 AAHNPAVKAGVAWYGRLVGDSSALMPKNPVDVAADLKAPVLGLYGGADQGIPVATIDRMK 244

Query: 269 DLEKGLGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLVA 301
           +  +  G+    V++   GH F  +       + A+  +  L++W     VA
Sbjct: 245 EACRAAGKTCDFVVYPEAGHAFHADYRPSYRAEPAQDGWKRLQDWFRQYGVA 291

BLAST of Chy9G157200 vs. ExPASy TrEMBL
Match: A0A0A0KT04 (DLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G652280 PE=4 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 5.5e-178
Identity = 305/310 (98.39%), Postives = 307/310 (99.03%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYNNMGVWRWKGKLRYSVE 60
           MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKY+NMGVWRWKGKLRYSVE
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGKLRYSVE 60

Query: 61  GRMK-GDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSI 120
           GRMK  DDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSI
Sbjct: 61  GRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSI 120

Query: 121 RDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAG 180
           RDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAG
Sbjct: 121 RDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAG 180

Query: 181 LSMKLGIIGFGYGGGRVIDVLARDEGACFLIGASIYGTQVDPSLATKVKVPVLLISGDND 240
           LSMKLGIIGFGYGGGRVIDVLARDEG CFLIGASIYGTQVDPSLATKVKVPVLLISGDND
Sbjct: 181 LSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLISGDND 240

Query: 241 PLCPVSVMKDLEKGLGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV 300
           PLCPVSVMKDLEKG+GEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV
Sbjct: 241 PLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV 300

Query: 301 AINNLANCHG 310
           AINNLANCHG
Sbjct: 301 AINNLANCHG 310

BLAST of Chy9G157200 vs. ExPASy TrEMBL
Match: A0A5A7TB03 (Carboxymethylenebutenolidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00030 PE=4 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.2e-162
Identity = 281/310 (90.65%), Postives = 295/310 (95.16%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYNNMGVWRWKGKLR-YSV 60
           MGFASAGAQLISLPPTGSSSRRV KP QPPY +FAYPLPS KYNN G W+WKGKLR YSV
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60

Query: 61  EGRMKGDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSI 120
           +GR+K DDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQ SSI
Sbjct: 61  KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI 120

Query: 121 RDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAG 180
           RDFAYRLAC GFNVLLPDLV+ED WEKPPA+H AKQEMQRIAEDIGTVTKWLT+EFSAAG
Sbjct: 121 RDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAAG 180

Query: 181 LSMKLGIIGFGYGGGRVIDVLARDEGACFLIGASIYGTQVDPSLATKVKVPVLLISGDND 240
           LS KLGIIGFG+GGGRVIDVLARD+GACFLIGAS+YGT+VDPSLATKVKVPVLLISGD+D
Sbjct: 181 LSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDD 240

Query: 241 PLCPVSVMKDLEKGLGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV 300
           PLCPVSVMKDLEKG+GEGSKLVIF GRGHGFVHNPGSPEEDDDAE+AFLMLRNWLHHGLV
Sbjct: 241 PLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGLV 300

Query: 301 AINNLANCHG 310
           A NNLANCHG
Sbjct: 301 ATNNLANCHG 310

BLAST of Chy9G157200 vs. ExPASy TrEMBL
Match: A0A1S3CB89 (uncharacterized protein LOC103498731 OS=Cucumis melo OX=3656 GN=LOC103498731 PE=4 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.2e-162
Identity = 281/310 (90.65%), Postives = 295/310 (95.16%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYNNMGVWRWKGKLR-YSV 60
           MGFASAGAQLISLPPTGSSSRRV KP QPPY +FAYPLPS KYNN G W+WKGKLR YSV
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60

Query: 61  EGRMKGDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSI 120
           +GR+K DDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQ SSI
Sbjct: 61  KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI 120

Query: 121 RDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAG 180
           RDFAYRLAC GFNVLLPDLV+ED WEKPPA+H AKQEMQRIAEDIGTVTKWLT+EFSAAG
Sbjct: 121 RDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAAG 180

Query: 181 LSMKLGIIGFGYGGGRVIDVLARDEGACFLIGASIYGTQVDPSLATKVKVPVLLISGDND 240
           LS KLGIIGFG+GGGRVIDVLARD+GACFLIGAS+YGT+VDPSLATKVKVPVLLISGD+D
Sbjct: 181 LSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDD 240

Query: 241 PLCPVSVMKDLEKGLGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV 300
           PLCPVSVMKDLEKG+GEGSKLVIF GRGHGFVHNPGSPEEDDDAE+AFLMLRNWLHHGLV
Sbjct: 241 PLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGLV 300

Query: 301 AINNLANCHG 310
           A NNLANCHG
Sbjct: 301 ATNNLANCHG 310

BLAST of Chy9G157200 vs. ExPASy TrEMBL
Match: A0A6J1DNR6 (hydrolase tropI OS=Momordica charantia OX=3673 GN=LOC111022362 PE=4 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 5.1e-115
Identity = 217/309 (70.23%), Postives = 245/309 (79.29%), Query Frame = 0

Query: 1   MGFASAG-AQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYNNMGVWRWKGKL---- 60
           MG ASAG AQ+ISL         +G PF         PL  RK  NMGVW+WK K     
Sbjct: 1   MGLASAGAAQMISL--------HIGTPFPS-------PLHLRKC-NMGVWKWKVKREKKG 60

Query: 61  -RYSVEGRMKGDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAF 120
            +     R++G+ D+ CELV+GMELSIGEQP  ++PA+LFKAVKNNNGTAILLLSDLFAF
Sbjct: 61  GQVCSSHRLEGEIDEGCELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAF 120

Query: 121 QHSSIRDFAYRLACIGFNVLLPDLVHEDVW-EKPPAEHCAKQEMQRIAEDIGTVTKWLTE 180
           Q SSI DFAYR+AC GFNVLLPD+ H D W +K P E  AKQ+MQR+AEDIGT TKWL E
Sbjct: 121 QDSSITDFAYRVACNGFNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVE 180

Query: 181 EFSAAGLSMKLGIIGFGYGGGRVIDVLARDEGACFLIGASIYGTQVDPSLATKVKVPVLL 240
           EFSAAG+S KLGI+GFGYGGG+VIDVLARD+GACF IGAS YGT +DPSL ++VKVPVLL
Sbjct: 181 EFSAAGISTKLGIMGFGYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLL 240

Query: 241 ISGDNDPLCPVSVMKDLEKGLGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNW 300
           ISGDNDPLCPVSVMKDLEKG+G+GSKLVIFGGRGHGF H PGSPEED+DAE+AFLMLRNW
Sbjct: 241 ISGDNDPLCPVSVMKDLEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNW 293

Query: 301 LHHGLVAIN 303
           LH GLV  N
Sbjct: 301 LHDGLVVTN 293

BLAST of Chy9G157200 vs. ExPASy TrEMBL
Match: A0A6J1EC40 (carboxymethylenebutenolidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111432716 PE=4 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 5.1e-115
Identity = 217/308 (70.45%), Postives = 251/308 (81.49%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYNNMGVWRWK-----GKL 60
           MG A+AGAQ IS PPTG S RR+G PFQP  F+FA+P+     N++G  + K     GK+
Sbjct: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQPS-FRFAFPIS----NSIGQLKGKREIKGGKV 60

Query: 61  RYSVEG-RMKGDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAF 120
            YS EG + + ++++ACELVNGMELSIGE+ HHSI AYLFKAVKNNNGTAILLL+DLFAF
Sbjct: 61  CYSAEGVKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLFAF 120

Query: 121 QHSSIRDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEE 180
           Q SS+RDFAYRLAC GFNVLLPD+ H D  E+           Q+  EDI   TKWL +E
Sbjct: 121 QDSSVRDFAYRLACNGFNVLLPDVFHGDSCEE-----------QKTVEDIAAATKWLVDE 180

Query: 181 FSAAGLSMKLGIIGFGYGGGRVIDVLARDEGACFLIGASIYGTQVDPSLATKVKVPVLLI 240
           FSAAG+S KLGI+GFGYGGGRVIDVLA+D+G+ F IGAS+YGT+V+PSLATKV+VPVLLI
Sbjct: 181 FSAAGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPVLLI 240

Query: 241 SGDNDPLCPVSVMKDLEKGLGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWL 300
           SGDNDPLCPVSVMKDLEKG+GEGSKLVIFGGRGHGFVH PGSPEED+DAE+AFLMLRNWL
Sbjct: 241 SGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWL 292

Query: 301 HHGLVAIN 303
           H GLVA N
Sbjct: 301 HDGLVATN 292

BLAST of Chy9G157200 vs. NCBI nr
Match: XP_004141569.1 (uncharacterized protein LOC101210518 [Cucumis sativus] >KGN52723.1 hypothetical protein Csa_008898 [Cucumis sativus])

HSP 1 Score: 627 bits (1617), Expect = 1.00e-226
Identity = 305/310 (98.39%), Postives = 307/310 (99.03%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYNNMGVWRWKGKLRYSVE 60
           MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKY+NMGVWRWKGKLRYSVE
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGKLRYSVE 60

Query: 61  GRMKGDDDD-ACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSI 120
           GRMK DDDD ACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSI
Sbjct: 61  GRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSI 120

Query: 121 RDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAG 180
           RDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAG
Sbjct: 121 RDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAG 180

Query: 181 LSMKLGIIGFGYGGGRVIDVLARDEGACFLIGASIYGTQVDPSLATKVKVPVLLISGDND 240
           LSMKLGIIGFGYGGGRVIDVLARDEG CFLIGASIYGTQVDPSLATKVKVPVLLISGDND
Sbjct: 181 LSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLISGDND 240

Query: 241 PLCPVSVMKDLEKGLGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV 300
           PLCPVSVMKDLEKG+GEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV
Sbjct: 241 PLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV 300

Query: 301 AINNLANCHG 309
           AINNLANCHG
Sbjct: 301 AINNLANCHG 310

BLAST of Chy9G157200 vs. NCBI nr
Match: XP_008459689.1 (PREDICTED: uncharacterized protein LOC103498731 [Cucumis melo] >KAA0039216.1 carboxymethylenebutenolidase-like protein [Cucumis melo var. makuwa] >TYK00404.1 carboxymethylenebutenolidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 575 bits (1481), Expect = 5.40e-206
Identity = 281/310 (90.65%), Postives = 295/310 (95.16%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYNNMGVWRWKGKLRY-SV 60
           MGFASAGAQLISLPPTGSSSRRV KP QPPY +FAYPLPS KYNN G W+WKGKLRY SV
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60

Query: 61  EGRMKGDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSI 120
           +GR+K DDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQ SSI
Sbjct: 61  KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI 120

Query: 121 RDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAAG 180
           RDFAYRLAC GFNVLLPDLV+ED WEKPPA+H AKQEMQRIAEDIGTVTKWLT+EFSAAG
Sbjct: 121 RDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAAG 180

Query: 181 LSMKLGIIGFGYGGGRVIDVLARDEGACFLIGASIYGTQVDPSLATKVKVPVLLISGDND 240
           LS KLGIIGFG+GGGRVIDVLARD+GACFLIGAS+YGT+VDPSLATKVKVPVLLISGD+D
Sbjct: 181 LSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDD 240

Query: 241 PLCPVSVMKDLEKGLGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV 300
           PLCPVSVMKDLEKG+GEGSKLVIF GRGHGFVHNPGSPEEDDDAE+AFLMLRNWLHHGLV
Sbjct: 241 PLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGLV 300

Query: 301 AINNLANCHG 309
           A NNLANCHG
Sbjct: 301 ATNNLANCHG 310

BLAST of Chy9G157200 vs. NCBI nr
Match: XP_038890567.1 (hydrolase tropI [Benincasa hispida])

HSP 1 Score: 494 bits (1272), Expect = 3.61e-174
Identity = 251/312 (80.45%), Postives = 268/312 (85.90%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSS--RRVGKPFQPPYFKFAYPLPSRKYNNMGVWRWKGKLR-- 60
           MG ASAGAQLISLPPTGSSS  RR+G PFQ PYF+F  PLP RK  N   W+WKGK    
Sbjct: 1   MGLASAGAQLISLPPTGSSSCRRRIGTPFQLPYFRFTSPLPFRKCGNRSDWKWKGKREKK 60

Query: 61  -----YSVEGRMKGDDDDA-CELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD 120
                YSVEG    DDDDA CELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD
Sbjct: 61  GGKVCYSVEGVEDDDDDDAACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD 120

Query: 121 LFAFQHSSIRDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKW 180
           LFAFQHSSIRDFAYRLAC GFNVLLPDL+H D W+K       K++MQRIAEDIGTVTKW
Sbjct: 121 LFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDSWKK----QSTKRDMQRIAEDIGTVTKW 180

Query: 181 LTEEFSAAGLSMKLGIIGFGYGGGRVIDVLARDEGACFLIGASIYGTQVDPSLATKVKVP 240
           L +EFSAAGLS KLGI+GFGYGGG+VI+VLARDEGACF IGAS+YGT+VDPSLATKVKVP
Sbjct: 181 LVDEFSAAGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASLYGTEVDPSLATKVKVP 240

Query: 241 VLLISGDNDPLCPVSVMKDLEKGLGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLML 300
           VLLISGDNDPLCPVSVMKDLEK +GEGSKLVIFGGRGH FVHNP SPEED+DAE+AFLM+
Sbjct: 241 VLLISGDNDPLCPVSVMKDLEKRMGEGSKLVIFGGRGHDFVHNPRSPEEDEDAEQAFLMI 300

Query: 301 RNWLHHGLVAIN 302
           RNWLHHGLVA N
Sbjct: 301 RNWLHHGLVATN 308

BLAST of Chy9G157200 vs. NCBI nr
Match: XP_023536049.1 (carboxymethylenebutenolidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 426 bits (1095), Expect = 2.05e-147
Identity = 222/308 (72.08%), Postives = 251/308 (81.49%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYNNMGVWRWK-----GKL 60
           MG A+AGAQ IS PPTG S RR+G PFQP  F+FA+P+     NN+G  + K     GKL
Sbjct: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQPS-FRFAFPIS----NNIGQLKGKREKKGGKL 60

Query: 61  RYSVEG-RMKGDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAF 120
            YS EG + + D+++ACELVNGMELSIGE+ HHSI AYLFKAVKNNNGTAILLL+DLFAF
Sbjct: 61  CYSAEGVKDEIDEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLFAF 120

Query: 121 QHSSIRDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEE 180
           Q SSIRDFAYRLAC GFNVLLP++ H D             E Q+I EDIG  TKWL +E
Sbjct: 121 QDSSIRDFAYRLACNGFNVLLPNIFHGD-----------SSEEQKIVEDIGAATKWLVDE 180

Query: 181 FSAAGLSMKLGIIGFGYGGGRVIDVLARDEGACFLIGASIYGTQVDPSLATKVKVPVLLI 240
           FSAAG+S KLGI+GFGYGGGRVIDVLARD+G+ F IGAS+YGT+VDPSLATKV+VPVLLI
Sbjct: 181 FSAAGISRKLGIVGFGYGGGRVIDVLARDQGSYFSIGASLYGTEVDPSLATKVEVPVLLI 240

Query: 241 SGDNDPLCPVSVMKDLEKGLGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWL 300
           SGDND LCPVSVMKDLEKG+G+GSKLVIFGGRGHGFVH PGSPEED+DAE+AFLMLRNWL
Sbjct: 241 SGDNDTLCPVSVMKDLEKGMGDGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWL 292

Query: 301 HHGLVAIN 302
           H GLVA N
Sbjct: 301 HDGLVATN 292

BLAST of Chy9G157200 vs. NCBI nr
Match: XP_022155217.1 (hydrolase tropI [Momordica charantia])

HSP 1 Score: 419 bits (1076), Expect = 1.43e-144
Identity = 217/309 (70.23%), Postives = 245/309 (79.29%), Query Frame = 0

Query: 1   MGFASAGA-QLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYNNMGVWRWKGKL---- 60
           MG ASAGA Q+ISL         +G PF         PL  RK N MGVW+WK K     
Sbjct: 1   MGLASAGAAQMISL--------HIGTPFPS-------PLHLRKCN-MGVWKWKVKREKKG 60

Query: 61  -RYSVEGRMKGDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAF 120
            +     R++G+ D+ CELV+GMELSIGEQP  ++PA+LFKAVKNNNGTAILLLSDLFAF
Sbjct: 61  GQVCSSHRLEGEIDEGCELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAF 120

Query: 121 QHSSIRDFAYRLACIGFNVLLPDLVHEDVWEKP-PAEHCAKQEMQRIAEDIGTVTKWLTE 180
           Q SSI DFAYR+AC GFNVLLPD+ H D W+K  P E  AKQ+MQR+AEDIGT TKWL E
Sbjct: 121 QDSSITDFAYRVACNGFNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVE 180

Query: 181 EFSAAGLSMKLGIIGFGYGGGRVIDVLARDEGACFLIGASIYGTQVDPSLATKVKVPVLL 240
           EFSAAG+S KLGI+GFGYGGG+VIDVLARD+GACF IGAS YGT +DPSL ++VKVPVLL
Sbjct: 181 EFSAAGISTKLGIMGFGYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLL 240

Query: 241 ISGDNDPLCPVSVMKDLEKGLGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNW 300
           ISGDNDPLCPVSVMKDLEKG+G+GSKLVIFGGRGHGF H PGSPEED+DAE+AFLMLRNW
Sbjct: 241 ISGDNDPLCPVSVMKDLEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNW 293

Query: 301 LHHGLVAIN 302
           LH GLV  N
Sbjct: 301 LHDGLVVTN 293

BLAST of Chy9G157200 vs. TAIR 10
Match: AT1G35420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 276.9 bits (707), Expect = 1.9e-74
Identity = 131/239 (54.81%), Postives = 174/239 (72.80%), Query Frame = 0

Query: 66  DDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDFAYR 125
           ++++ACELVNG E+S+       +  YL  AVKNNNGT +LLLSD+F FQ S+ RDFAYR
Sbjct: 77  EEEEACELVNGTEVSV-----DGVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDFAYR 136

Query: 126 LACIGFNVLLPDLVHEDVWEK--PPAEH---CAKQEMQRIAEDIGTVTKWLTEEFSAAGL 185
           +AC G+NVL+PDL   D W K  P +E+       +  RI +D  T TKW+ EEF+AAG+
Sbjct: 137 VACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWMVEEFAAAGI 196

Query: 186 SMKLGIIGFGYGGGRVIDVLARDEGACFLIGASIYGTQVDPSLATKVKVPVLLISGDNDP 245
           S KLG++GF +GGGRV+DVLA DE   F  G S YGT++D ++A  VKVPVL I+GD DP
Sbjct: 197 SKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSAVAGDVKVPVLFIAGDRDP 256

Query: 246 LCPVSVMKDLEKGLGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV 300
           LC V  + ++E+ +GEGSK+V++ GRGHGFVH P +PE+D DAE+AF ++RNWLHH L+
Sbjct: 257 LCEVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRDAEEAFALMRNWLHHHLI 310

BLAST of Chy9G157200 vs. TAIR 10
Match: AT1G35420.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 276.9 bits (707), Expect = 1.9e-74
Identity = 131/239 (54.81%), Postives = 174/239 (72.80%), Query Frame = 0

Query: 66  DDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDFAYR 125
           ++++ACELVNG E+S+       +  YL  AVKNNNGT +LLLSD+F FQ S+ RDFAYR
Sbjct: 82  EEEEACELVNGTEVSV-----DGVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDFAYR 141

Query: 126 LACIGFNVLLPDLVHEDVWEK--PPAEH---CAKQEMQRIAEDIGTVTKWLTEEFSAAGL 185
           +AC G+NVL+PDL   D W K  P +E+       +  RI +D  T TKW+ EEF+AAG+
Sbjct: 142 VACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWMVEEFAAAGI 201

Query: 186 SMKLGIIGFGYGGGRVIDVLARDEGACFLIGASIYGTQVDPSLATKVKVPVLLISGDNDP 245
           S KLG++GF +GGGRV+DVLA DE   F  G S YGT++D ++A  VKVPVL I+GD DP
Sbjct: 202 SKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSAVAGDVKVPVLFIAGDRDP 261

Query: 246 LCPVSVMKDLEKGLGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGLV 300
           LC V  + ++E+ +GEGSK+V++ GRGHGFVH P +PE+D DAE+AF ++RNWLHH L+
Sbjct: 262 LCEVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRDAEEAFALMRNWLHHHLI 315

BLAST of Chy9G157200 vs. TAIR 10
Match: AT3G23600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 50.4 bits (119), Expect = 2.9e-06
Identity = 49/198 (24.75%), Postives = 93/198 (46.97%), Query Frame = 0

Query: 105 ILLLSDLFAFQHSSIRDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDI 164
           +LL+SD+F F+  ++R  A ++A  GF V++PD    D +     +      ++    D 
Sbjct: 42  VLLISDIFGFEAPNLRALADKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDK 101

Query: 165 G-TVTKWLTEEFSAAGLSMKLGIIGFGYGGGRVIDVLARDEGACFLIGASI--YGTQVDP 224
           G   TK + E     G++  +G  G  + G +V+  L+++E    LI A++  + + V+ 
Sbjct: 102 GFENTKPVLETIKNKGIT-AIGAAGMCW-GAKVVVELSKEE----LIQAAVLLHPSFVNV 161

Query: 225 SLATKVKVPVLLISGDNDPLCPVSVMKDLEKGLGE----GSKLVIFGGRGHGFV--HNPG 284
                 K P+ ++  + D + P +++K  E+ L       S + I     HG+   +N  
Sbjct: 162 DDIKGGKAPIAILGAEIDQMSPPALLKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNID 221

Query: 285 SPEEDDDAEKAFLMLRNW 294
            PE    AE+A   + +W
Sbjct: 222 EPEAVKAAEEAHKEMLDW 233

BLAST of Chy9G157200 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 43.1 bits (100), Expect = 4.7e-04
Identity = 24/64 (37.50%), Postives = 38/64 (59.38%), Query Frame = 0

Query: 226 KVKVPVLLISGDNDPLCPVSVMKDLEKGLGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEK 285
           K+K P L+I G++D + P+ + K LEK +G+  KLVI    GH  + N   P++     K
Sbjct: 241 KLKQPTLIIWGEHDQVFPLEMGKRLEKHVGDNGKLVIIKRTGH--IFNFEKPKKFIKLLK 300

Query: 286 AFLM 290
           +FL+
Sbjct: 301 SFLL 302