Chy8G156810 (gene) Cucumber (hystrix) v1

Overview


Name	Chy8G156810
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	nicotianamine synthase-like
Location	chrH08: 17916883 .. 17920833 (+)
RNA-Seq Expression	Chy8G156810
Synteny	Chy8G156810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTTGTGAAGAAGAAACTCTAATCCAAAAGGTTTGTGAATTATATGATGAAATCTCAAGCCTTGAAAGCCTCAAACCTTGTAACAATGTCGACACTCTTTTCACTAAGTTGGTGGTCACGTGCATGTCACCCACCTCCCCTCATTTCCATATCAATTCACTCTCAAAACCACTTCAACAGATGAGATCAAATCTCATTCGTCTCTGTGGTCAAGCTGAGTCTCTTTTAGAGTTTCATTTTTCTGATTTATTGGCCAAATTTGATTCCCCTATCGACCATCTTCATGTCTTCCCTTACTTCTCTAATTACATTAAACTTAGCCTCCTTGAATTCTCTATTCTCCGCTCCCATGGCCCCCTCGCGCTTCCCTCCGCCGTTGCATTCGTTGGCTCTGGCCCTCTTCCTCTTACCTCCGTCGTCCTTGCCACTCGCCACCTCACCTCCACCATCTTCCACAACTACGACCTCGACCCGGTTGCTAATTCAAAGGTTTGTATATCGTCTTTGCAAGAAAAATTATATGAGTTTGCTAGTTTGGTTAGTTGATTTGTTTGTTGCAATAATTATCCATTTATGTTTGTGAGGTTTTGAATTTTGAAGTGATTTAATATGGTATTAGGAAAAAAATTCTTAACCCTCCGATTGATTTTGGGAATCACAAATGATTTCTGTTTTATAAATTTATATGTTTGTTTCTATCTCTTAATTTTTCAATTATGGTTTTCATGTTTTAAAGAAGCCTGAAAGTTCTTGATAACTTCCTAGAACAAAAACTATTTTTGTTGGAAACTGTTTTTTAGACCCTTTGGATAATTATTTGATTTTTTTTGTTTTTCATAATTTTCTAACAATATTTAGGCTTATAAACATTGTTTCCCCCTTTATTCTTGCTTTGTTATCTATTTTTTACTAGTATTTTAAAAAACAAAGTCAAGTTTTGATACTTAAAAAAAAAGTCTTTTAAAAACTACTTATTTTGTTTTTGAAATTAGGTTAAAAGTATGACTATCATGACAAGGAAAAATGGAAAACATTGTATTGTATATAAAATTAAGGGAAACGAAGGCTAATTTTCAAAAATCTAGTTATCAAATAGTGTATCTTTAAAACATGGTTTTTGAAAAGATAACAAAACATTGAAACTTATTGTTTTGAGAATGAAATTAATGCTCATAAGATTACTTCGTTAAAAAAAACCAAATATTTTTCAAACGGGAACGCAATGGAGTACTAATAAACTTGTATTTTCTTTCCACAGGCATCGAACTTGATGTCGCGTGACCCCGACCTGAAGACAAGGATGGTGTTTCACACGTGCGACATCATGAAAGTGACAGAGGAGCTAAAACAGTATGAGGTTGTGTTTTTGGCAGCTCTGGTAGGAATGGAGAAGGAGGAAAAACTAAAGGTGATCAAGCATTTGTCAGAGTACATGTCAGAAGGAGCTTATTTGATGGTAAGAAGTGCTCATGGAGGAAGAGCTTTTTTATATCCTGTGGTTGAAGATTCTGATTTGTTAGGGTTTGAGGTTCTATCTGTGTTTCATCCCACTGATGAAGTTATCAACTCAGTAATAATTGCAAGAAAAACAACGAAATTCGATAACGACAACGACAGTGACGACAACAACAACAACGACAACGGTTGTTTGTTTGTCAATTCAGATGAAAATGAAAATGTGAAGATTAGTAGTGGTATTGATCATAAGAAGGATAATAATGGTGTTGTTCATAATAACAAATGTTCTGAGATCCAAAACGGCTTCAATAATCATGGAGGTAAGATCGAGGAATTTGCAATGGAAGCAGCTGAATAATAATAATAATAATAATAATAACAATTCACCCACTTGAATAATTATCGTTCAGTGGGGGTTTCTGATTCTGATTCTCAAAATTATTATGTGTTCCTCTTTTTTTTTCAACATGGGAAAATAATTTTGATCATTTTTCATATTTGAGGAACACATTCCTGGGATAATATAAGGAGCTAGCTTATATATAGCAAAGGTTTTATATATGTTTTGATTAATGTTGTTGTATTCTAGAGTCTAATTGGGTTATTCTCTATAGCAAATGGAGAACTTTTAATTTGTTATATGTTTAATTTGTAATAATTTTTGTGTTCTCACTTTTGGATGAGAATCTAAACTATACAATTAGATGGTTTTTGTTATTTTGTCAGGATATACTCTTTTAAAGTGTTTATTATTTAGGGTAATTTTGTTTTTAATTACTCTAATTGTGTGATTCAAATTTATGTTTTTGAGTAGGATTCTCCTTAAAGAAAATATGCTTAAATTCTTAGTTAATTTTTAAAAATATTAATGAAAAGTGAAGTAAGTGAAAATAGTATTTCTATTAATTAACTTACTTTTTCAAAAATTAAAAATCAAATGGTGATTTTATTTAGAAAACAAAATTAAACTGAAAATTAATTTTCATTTGACCAAAGTTCACAACATGAAGGGGTTAAATGAATAGAGCTAGGGTTTTGCATCAAATAACATAATATTCTGTTTTAACTTTTTTTTTTCCTCATTGATTAAACTTGGGAAAATATATAATTAGGTTAATTTCTTCAATAAGTTGATTTGGTTATTAATAAATTATTCAAATTATTTTGAATAGGCTCCCCTCCTCCTAGTTTTTAGTTTAAAATTAAGTTTAGAAAAATATTAAGATTTTCTTTCTTTAATGGTTTGTTGTTTAAAAAAAAAAAAAAAAAACTTTATTTGTTGTCTACATTTTACGAGTGTTTTTTAAAATTCAAGCTAAGGTTTTTAAACTCTATTAGTTTTGAAAAATAAGTCTATTTTCTCGTAGAGTTTCTAGAAACTCAAGGAAATTTGTCGTAGAACATAAACAAATCTTGTTCAAGTGCTCGTTGGTACATATTAATATATATATATATATGTATACATACAGACATGTAAACTAAGGAAAGGTAAATGTGGTAATAGATCATTTTTCTTCTATGGATAATGTATAAAAACAATAAACCTAATAGAAAATAAGGATACAAACAACATTTGGCAGAAAACTAGGTGAAATATTACTTATATGCACATTTTTTCACGTAAATGGTAAAAAATATTAAATTTGACAAAATATTTATAACATATAGCAAAATTTTAGATTATATCCATAATAAATATTGATAGACACTGATATGCTTCTATCAATTTCTATTATTGATAGAATCCAAAATTATTGGAATATGATTCATGGTGTTATTAACTAGATCCTAGATTTAAGATGTGATTATTCAAATTTAATTTCTTGAATATTTATGAAAAATATATTGATCGATTTGTGTTTTGTTCAAATTCAAGTATAGGAGAACCTATAGTTTTTTCTTCAACTACAAGGCGAGACATAAAAAATGATCAATTTCTCAAGTTTAATGAGTGTATTGTTGATGTGGAAAAAGTTTACAATGTCAAATCGAAGTTGAATCATTATTTAGATAGGAGAATCTTCTGCCTTGAAAATTGATAACTTTAACGTATAAATTAAACATTTTAATTATAACTTTGCATCTACTATATTATTGATTTTGTTAGAAAGTTCTATAATGTGTGGGTTAAACGAAAAATGTATTGAAAAATGTTCTATTTAAAAGAACTAAAATTTTTTAGTTGAAATTAAATGAATTATGACAAAAAAGAAATTAAGATCGAAGAATTTTTCTAAAATTTTTGTGTCGATGCAAACCAGCCATCAAAATAAATATTAACGAGCGTCTGATCTACCAACTTCTTAAATTACAAATAAAATTCACAAGTAAATTGCTAAGTGTATAGTTTTTTTCTTCCTATTATCAAAGAACTTCGTAATTCTACATGAAGATAGAAGCTTAACCAACTTCAATTCTAAAGTAATTAACTCTTTCCACTTAAATTATCCGAAAAATCCCAAATTACCCAACGAACTTTCCAACGGTTAA

mRNA sequence

ATGGTTTGTGAAGAAGAAACTCTAATCCAAAAGGTTTGTGAATTATATGATGAAATCTCAAGCCTTGAAAGCCTCAAACCTTGTAACAATGTCGACACTCTTTTCACTAAGTTGGTGGTCACGTGCATGTCACCCACCTCCCCTCATTTCCATATCAATTCACTCTCAAAACCACTTCAACAGATGAGATCAAATCTCATTCGTCTCTGTGGTCAAGCTGAGTCTCTTTTAGAGTTTCATTTTTCTGATTTATTGGCCAAATTTGATTCCCCTATCGACCATCTTCATGTCTTCCCTTACTTCTCTAATTACATTAAACTTAGCCTCCTTGAATTCTCTATTCTCCGCTCCCATGGCCCCCTCGCGCTTCCCTCCGCCGTTGCATTCGTTGGCTCTGGCCCTCTTCCTCTTACCTCCGTCGTCCTTGCCACTCGCCACCTCACCTCCACCATCTTCCACAACTACGACCTCGACCCGGTTGCTAATTCAAAGGCATCGAACTTGATGTCGCGTGACCCCGACCTGAAGACAAGGATGGTGTTTCACACGTGCGACATCATGAAAGTGACAGAGGAGCTAAAACAGTATGAGGTTGTGTTTTTGGCAGCTCTGGTAGGAATGGAGAAGGAGGAAAAACTAAAGGTGATCAAGCATTTGTCAGAGTACATGTCAGAAGGAGCTTATTTGATGATTAGTAGTGGTATTGATCATAAGAAGGATAATAATGGTGTTGTTCATAATAACAAATGTTCTGAGATCCAAAACGGCTTCAATAATCATGGAGGATATACTCTTTTAAAGTGTTTATTATTTAGGAACTTCGTAATTCTACATGAAGATAGAAGCTTAACCAACTTCAATTCTAAAGTAATTAACTCTTTCCACTTAAATTATCCGAAAAATCCCAAATTACCCAACGAACTTTCCAACGGTTAA

Coding sequence (CDS)

ATGGTTTGTGAAGAAGAAACTCTAATCCAAAAGGTTTGTGAATTATATGATGAAATCTCAAGCCTTGAAAGCCTCAAACCTTGTAACAATGTCGACACTCTTTTCACTAAGTTGGTGGTCACGTGCATGTCACCCACCTCCCCTCATTTCCATATCAATTCACTCTCAAAACCACTTCAACAGATGAGATCAAATCTCATTCGTCTCTGTGGTCAAGCTGAGTCTCTTTTAGAGTTTCATTTTTCTGATTTATTGGCCAAATTTGATTCCCCTATCGACCATCTTCATGTCTTCCCTTACTTCTCTAATTACATTAAACTTAGCCTCCTTGAATTCTCTATTCTCCGCTCCCATGGCCCCCTCGCGCTTCCCTCCGCCGTTGCATTCGTTGGCTCTGGCCCTCTTCCTCTTACCTCCGTCGTCCTTGCCACTCGCCACCTCACCTCCACCATCTTCCACAACTACGACCTCGACCCGGTTGCTAATTCAAAGGCATCGAACTTGATGTCGCGTGACCCCGACCTGAAGACAAGGATGGTGTTTCACACGTGCGACATCATGAAAGTGACAGAGGAGCTAAAACAGTATGAGGTTGTGTTTTTGGCAGCTCTGGTAGGAATGGAGAAGGAGGAAAAACTAAAGGTGATCAAGCATTTGTCAGAGTACATGTCAGAAGGAGCTTATTTGATGATTAGTAGTGGTATTGATCATAAGAAGGATAATAATGGTGTTGTTCATAATAACAAATGTTCTGAGATCCAAAACGGCTTCAATAATCATGGAGGATATACTCTTTTAAAGTGTTTATTATTTAGGAACTTCGTAATTCTACATGAAGATAGAAGCTTAACCAACTTCAATTCTAAAGTAATTAACTCTTTCCACTTAAATTATCCGAAAAATCCCAAATTACCCAACGAACTTTCCAACGGTTAA

Protein sequence

MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQQMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILRSHGPLALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTRMVFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISSGIDHKKDNNGVVHNNKCSEIQNGFNNHGGYTLLKCLLFRNFVILHEDRSLTNFNSKVINSFHLNYPKNPKLPNELSNG*

Homology

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BLAST of Chy8G156810 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 8.8e-72
Identity = 132/232 (56.90%), Postives = 182/232 (78.45%), Query Frame = 0


Query: 3   CEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQQM 62
           C+++ L+ K+C+LY++IS LE+LKPC +VDTLF +LV TC+ P +P+  +  +S+ +Q+M
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCI-PPNPNIDVTKMSENIQEM 63

Query: 63  RSNLIRLCGQAESLLEFHFSDLLAKF-DSPIDHLHVFPYFSNYIKLSLLEFSILRSHGPL 122
           RSNLI++CG+AE  LE HFS +L  F D+P+ HL++FPY++NY+KLS LEF +L  +   
Sbjct: 64  RSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNG 123

Query: 123 ALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTRMVF 182
            +P  VAF+GSGPLPLTSVVLA+ HL  +IFHN+D+DP AN  A+ L+S DPDL  RM F
Sbjct: 124 FVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFF 183

Query: 183 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISS 234
           HT DIM VTE LK ++VVFLAALVGM+K+EK+KV++HL ++MS GA LM+ S
Sbjct: 184 HTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRS 234

[Close]

BLAST of Chy8G156810 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.8e-69
Identity = 133/234 (56.84%), Postives = 179/234 (76.50%), Query Frame = 0


Query: 1   MVC-EEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPL 60
           MVC     +++KVCELY++IS LE+L P  +V+ LFT LV TCM P      ++ L + +
Sbjct: 1   MVCPNSNPVVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNP--IDVSKLCQKI 60

Query: 61  QQMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILRSHG 120
           Q++RS+LI+LCGQAE LLE HFS +L+ +++P+ HLH+FPYF NYIKLSLLE++IL +  
Sbjct: 61  QEIRSHLIKLCGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNIL-TKN 120

Query: 121 PLALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTRM 180
              +P  +AF+GSGPLPLTS+VLAT+HL +T FHNYD+D  AN  AS L++ DPD+ +RM
Sbjct: 121 TTNIPKKIAFIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRM 180

Query: 181 VFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISS 234
            FHT D+M VT  LK Y+VVFLAALVGM+KE+K+KV+ HL++YMS GA LM+ S
Sbjct: 181 TFHTADVMDVTCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRS 231

[Close]

BLAST of Chy8G156810 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 7.7e-68
Identity = 127/236 (53.81%), Postives = 176/236 (74.58%), Query Frame = 0


Query: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           M C+   +++++ +LYD+IS L+SLKP  NVDTLF +LV TC+ PT  +  + ++ + ++
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL-PTDTNIDVTNMCEEVK 60

Query: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKF---DSPIDHLHVFPYFSNYIKLSLLEFSILRS 120
            MR+NLI+LCG+AE  LE HFS +L       +P+DHLH+FPY+SNY+KL  LEF +L  
Sbjct: 61  DMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQ 120

Query: 121 HGPLALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKT 180
           H    +P+ +AFVGSGP+PLTS+VLA  HL +T FHN+D+D  AN+ ASNL+SRDPDL  
Sbjct: 121 HSS-HVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSK 180

Query: 181 RMVFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISS 234
           RM+FHT D++  TE L QY+VVFLAALVGM+KE K+K I+HL ++M+ GA LM+ S
Sbjct: 181 RMIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRS 234

[Close]

BLAST of Chy8G156810 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.7e-67
Identity = 129/234 (55.13%), Postives = 177/234 (75.64%), Query Frame = 0


Query: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           M C++E L+Q +C+LY++IS LESLKP  +V+ LF +LV TC+ P +P+  +  +   +Q
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCI-PPNPNIDVTKMCDRVQ 60

Query: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKF-DSPIDHLHVFPYFSNYIKLSLLEFSILRSHG 120
           ++R NLI++CG AE  LE HFS +L  + D+P+ HL++FPY++NY+KL  LEF +L  + 
Sbjct: 61  EIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNL 120

Query: 121 PLALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTRM 180
              +P +VAF+GSGPLPLTS+VLA+ HL  TIFHN+D+DP ANS AS L+S DPD+  RM
Sbjct: 121 NGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRM 180

Query: 181 VFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISS 234
            FHT DIM VTE LK ++VVFLAALVGM KEEK+KVI+HL ++M+ GA LM+ S
Sbjct: 181 FFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRS 233

[Close]

BLAST of Chy8G156810 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 3.6e-65
Identity = 125/235 (53.19%), Postives = 176/235 (74.89%), Query Frame = 0


Query: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSL-SKPL 60
           M CE   +++++ +LY++IS+LESLKP  NVDTLF +LV TC+ PT  +  +  +  + +
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL-PTDTNIDVTEIHDEKV 60

Query: 61  QQMRSNLIRLCGQAESLLEFHFSDLLAKF-DSPIDHLHVFPYFSNYIKLSLLEFSILRSH 120
           + MRS+LI+LCG+AE  LE HFS +L  F D+P++HLH+FPY++NY+KL  LEF +L  H
Sbjct: 61  KDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQH 120

Query: 121 GPLALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTR 180
               +P+ VAF+GSGP+PLTS+VLA  HL +T FHN+D+D  AN+ ASNL+SRD DL  R
Sbjct: 121 -TTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKR 180

Query: 181 MVFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISS 234
           M+FHT D++   E L QY+VVFLAALVGM+KE K+K I+HL ++M+ GA +M+ S
Sbjct: 181 MIFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRS 233

[Close]

BLAST of Chy8G156810 vs. ExPASy TrEMBL
Match: A0A0A0LVX1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G561410 PE=3 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 4.9e-126
Identity = 249/330 (75.45%), Postives = 254/330 (76.97%), Query Frame = 0


Query: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ
Sbjct: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILRSHGP 120
           QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSIL  HGP
Sbjct: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILHRHGP 120

Query: 121 LALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTRMV 180
            ALPSAVAFVGSGPLPLTSVVLATRHLTST+FHNYDLDPVANSKASNL+SRDPDLKTRMV
Sbjct: 121 RALPSAVAFVGSGPLPLTSVVLATRHLTSTVFHNYDLDPVANSKASNLVSRDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISSG------ 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLM+ S       
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMVRSAHGGRAF 240

Query: 241 ---------------------------------------IDHKKDNN------------- 263
                                                   D+  D+N             
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTMKFDNDNDSDDNNNNDNGCCLFVNS 300

[Close]

BLAST of Chy8G156810 vs. ExPASy TrEMBL
Match: A0A1S3BPH1 (nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103492113 PE=3 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 1.1e-125
Identity = 248/338 (73.37%), Postives = 256/338 (75.74%), Query Frame = 0


Query: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MVCEEETLIQ+VCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ
Sbjct: 1   MVCEEETLIQRVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILRSHGP 120
           QMRS+LI LCGQAESLLE HFSDLLAKFDSPIDHLHVFPYFSNY+KLSLLEFSILR HGP
Sbjct: 61  QMRSSLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120

Query: 121 LALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTRMV 180
             LPSAVAFVGSGPLPLTS+VLATRHLTST+FHNYDLDPVANSKASNL+S DPDLKTRMV
Sbjct: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLM---------- 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEK+EKLKVIKHLS+YMSEGAYLM          
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240

Query: 241 ------------------------------------------------------------ 263
                                                                       
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDE 300

[Close]

BLAST of Chy8G156810 vs. ExPASy TrEMBL
Match: A0A6J1JPC1 (nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111486493 PE=3 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 6.0e-100
Identity = 206/317 (64.98%), Postives = 231/317 (72.87%), Query Frame = 0


Query: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MV EEE LIQKV +LY++IS LESLKPC +VDTLFT+LVVTCM PTSP+FHINSLS PLQ
Sbjct: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60

Query: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILRSHGP 120
           QMRSNLIRLCGQAESLLE HFSDLLA FD P+ HLH+FPYFSNY+KLSLLEFSILR H P
Sbjct: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 LALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTRMV 180
           + LPS+VAFVGSGPLPLTS+VLATRHL ST FHNYDLDP+ANS+AS L+S D DLKTRMV
Sbjct: 121 I-LPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISSG------ 240
           FHTCDIMKVTEELK+YEVVFLAALVGMEKEEKL+VI+HL E+M+EGA LM+ S       
Sbjct: 181 FHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAF 240

Query: 241 ------------------------------IDHKK-------------------DNNGVV 263
                                         I  KK                   +N  VV
Sbjct: 241 LYPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKKDDDCLKIVGDDNHENGIENGVVV 300

[Close]

BLAST of Chy8G156810 vs. ExPASy TrEMBL
Match: A0A6J1EU11 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111437742 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 1.0e-99
Identity = 205/316 (64.87%), Postives = 230/316 (72.78%), Query Frame = 0


Query: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MV EEE LIQKV +LY++IS LESLKPC +VDTLFT+LVVTCM PTSP+FHINSLS PLQ
Sbjct: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60

Query: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILRSHGP 120
           QMRSNLIRLCGQAESLLE HFSDLLA FD P+ HLH+FPYFSNY+KLSLLEFSILR H P
Sbjct: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 LALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTRMV 180
           + LPS+VAFVGSGPLPLTS+VLATRHL ST FHNYDLDP+AN++AS L+S D DLKTRMV
Sbjct: 121 V-LPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANTQASKLVSVDSDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISSG------ 240
           FHTCDIMKVTEELK+YEVVFLAALVGMEKEEKL+VI+HL E+M+EGA LM+ S       
Sbjct: 181 FHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAF 240

Query: 241 ------------------------------------IDHKKD------------NNGVVH 263
                                                DH  D            N  VVH
Sbjct: 241 LYPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKKDHDDDDCLKIVGDDNHENGVVVH 300

[Close]

BLAST of Chy8G156810 vs. ExPASy TrEMBL
Match: A0A5A7U6Y1 (Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004490 PE=3 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 2.2e-89
Identity = 188/277 (67.87%), Postives = 195/277 (70.40%), Query Frame = 0


Query: 62  MRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILRSHGPL 121
           MRS+LI LCGQAESLLE HFSDLLAKFDSPIDHLHVFPYFSNY+KLSLLEFSILR HGP 
Sbjct: 1   MRSSLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGPR 60

Query: 122 ALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTRMVF 181
            LPSAVAFVGSGPLPLTS+VLATRHLTST+FHNYDLDPVANSKASNL+S DPDLKTRMVF
Sbjct: 61  VLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMVF 120

Query: 182 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLM----------- 241
           HTCDIMKVTEELKQYEVVFLAALVGMEK+EKLKVIKHLS+YMSEGAYLM           
Sbjct: 121 HTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAFL 180

Query: 242 ------------------------------------------------------------ 263
                                                                       
Sbjct: 181 YPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDEN 240

[Close]

BLAST of Chy8G156810 vs. NCBI nr
Match: XP_004135580.1 (nicotianamine synthase [Cucumis sativus] >KGN66010.1 hypothetical protein Csa_007577 [Cucumis sativus])

HSP 1 Score: 464 bits (1193), Expect = 1.31e-161
Identity = 249/330 (75.45%), Postives = 254/330 (76.97%), Query Frame = 0


Query: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ
Sbjct: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILRSHGP 120
           QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSIL  HGP
Sbjct: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILHRHGP 120

Query: 121 LALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTRMV 180
            ALPSAVAFVGSGPLPLTSVVLATRHLTST+FHNYDLDPVANSKASNL+SRDPDLKTRMV
Sbjct: 121 RALPSAVAFVGSGPLPLTSVVLATRHLTSTVFHNYDLDPVANSKASNLVSRDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISSGI----- 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLM+ S       
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMVRSAHGGRAF 240

Query: 241 ----------------------------------------DHKKDNN------------- 262
                                                   D+  D+N             
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTMKFDNDNDSDDNNNNDNGCCLFVNS 300

[Close]

BLAST of Chy8G156810 vs. NCBI nr
Match: XP_008450545.1 (PREDICTED: nicotianamine synthase-like [Cucumis melo])

HSP 1 Score: 462 bits (1190), Expect = 4.97e-161
Identity = 248/338 (73.37%), Postives = 256/338 (75.74%), Query Frame = 0


Query: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MVCEEETLIQ+VCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ
Sbjct: 1   MVCEEETLIQRVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILRSHGP 120
           QMRS+LI LCGQAESLLE HFSDLLAKFDSPIDHLHVFPYFSNY+KLSLLEFSILR HGP
Sbjct: 61  QMRSSLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120

Query: 121 LALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTRMV 180
             LPSAVAFVGSGPLPLTS+VLATRHLTST+FHNYDLDPVANSKASNL+S DPDLKTRMV
Sbjct: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLM---------- 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEK+EKLKVIKHLS+YMSEGAYLM          
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240

Query: 241 ------------------------------------------------------------ 262
                                                                       
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDE 300

[Close]

BLAST of Chy8G156810 vs. NCBI nr
Match: XP_038878580.1 (probable nicotianamine synthase 4 [Benincasa hispida])

HSP 1 Score: 419 bits (1077), Expect = 1.51e-143
Identity = 213/233 (91.42%), Postives = 223/233 (95.71%), Query Frame = 0


Query: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MVCEEE LIQKV ELYDEISSLESLKPCN+VDTLFTKLVVTCMSPTSPHFHINSLSK LQ
Sbjct: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKSLQ 60

Query: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILRSHGP 120
           QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPI HLHVFPYFSNY+KLSLLEFSILR H P
Sbjct: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 LALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTRMV 180
           + LPSAVAFVGSGPLPLTS+VLATRHLTST+FHNYDLD +ANSKASNL+S DPDLKTRMV
Sbjct: 121 V-LPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDLLANSKASNLVSLDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISS 233
           FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKL+VIKHLSE+MSEGAYLM+ S
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIKHLSEFMSEGAYLMVRS 232

[Close]

BLAST of Chy8G156810 vs. NCBI nr
Match: XP_022989439.1 (nicotianamine synthase-like [Cucurbita maxima])

HSP 1 Score: 377 bits (968), Expect = 1.12e-127
Identity = 206/317 (64.98%), Postives = 231/317 (72.87%), Query Frame = 0


Query: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MV EEE LIQKV +LY++IS LESLKPC +VDTLFT+LVVTCM PTSP+FHINSLS PLQ
Sbjct: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60

Query: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILRSHGP 120
           QMRSNLIRLCGQAESLLE HFSDLLA FD P+ HLH+FPYFSNY+KLSLLEFSILR H P
Sbjct: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 LALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTRMV 180
           + LPS+VAFVGSGPLPLTS+VLATRHL ST FHNYDLDP+ANS+AS L+S D DLKTRMV
Sbjct: 121 I-LPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISSG------ 240
           FHTCDIMKVTEELK+YEVVFLAALVGMEKEEKL+VI+HL E+M+EGA LM+ S       
Sbjct: 181 FHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAF 240

Query: 241 ------------------------------IDHKK-------------------DNNGVV 262
                                         I  KK                   +N  VV
Sbjct: 241 LYPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKKDDDCLKIVGDDNHENGIENGVVV 300

[Close]

BLAST of Chy8G156810 vs. NCBI nr
Match: XP_022931567.1 (nicotianamine synthase-like [Cucurbita moschata])

HSP 1 Score: 376 bits (966), Expect = 2.17e-127
Identity = 205/316 (64.87%), Postives = 230/316 (72.78%), Query Frame = 0


Query: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MV EEE LIQKV +LY++IS LESLKPC +VDTLFT+LVVTCM PTSP+FHINSLS PLQ
Sbjct: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60

Query: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILRSHGP 120
           QMRSNLIRLCGQAESLLE HFSDLLA FD P+ HLH+FPYFSNY+KLSLLEFSILR H P
Sbjct: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 LALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTRMV 180
           + LPS+VAFVGSGPLPLTS+VLATRHL ST FHNYDLDP+AN++AS L+S D DLKTRMV
Sbjct: 121 V-LPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANTQASKLVSVDSDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISSGI----- 240
           FHTCDIMKVTEELK+YEVVFLAALVGMEKEEKL+VI+HL E+M+EGA LM+ S       
Sbjct: 181 FHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAF 240

Query: 241 -------------------------------------DHKKD------------NNGVVH 262
                                                DH  D            N  VVH
Sbjct: 241 LYPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKKDHDDDDCLKIVGDDNHENGVVVH 300

[Close]

BLAST of Chy8G156810 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 271.9 bits (694), Expect = 6.2e-73
Identity = 132/232 (56.90%), Postives = 182/232 (78.45%), Query Frame = 0


Query: 3   CEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQQM 62
           C+++ L+ K+C+LY++IS LE+LKPC +VDTLF +LV TC+ P +P+  +  +S+ +Q+M
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCI-PPNPNIDVTKMSENIQEM 63

Query: 63  RSNLIRLCGQAESLLEFHFSDLLAKF-DSPIDHLHVFPYFSNYIKLSLLEFSILRSHGPL 122
           RSNLI++CG+AE  LE HFS +L  F D+P+ HL++FPY++NY+KLS LEF +L  +   
Sbjct: 64  RSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNG 123

Query: 123 ALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTRMVF 182
            +P  VAF+GSGPLPLTSVVLA+ HL  +IFHN+D+DP AN  A+ L+S DPDL  RM F
Sbjct: 124 FVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFF 183

Query: 183 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISS 234
           HT DIM VTE LK ++VVFLAALVGM+K+EK+KV++HL ++MS GA LM+ S
Sbjct: 184 HTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRS 234

[Close]

BLAST of Chy8G156810 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 258.8 bits (660), Expect = 5.5e-69
Identity = 127/236 (53.81%), Postives = 176/236 (74.58%), Query Frame = 0


Query: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           M C+   +++++ +LYD+IS L+SLKP  NVDTLF +LV TC+ PT  +  + ++ + ++
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL-PTDTNIDVTNMCEEVK 60

Query: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKF---DSPIDHLHVFPYFSNYIKLSLLEFSILRS 120
            MR+NLI+LCG+AE  LE HFS +L       +P+DHLH+FPY+SNY+KL  LEF +L  
Sbjct: 61  DMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQ 120

Query: 121 HGPLALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKT 180
           H    +P+ +AFVGSGP+PLTS+VLA  HL +T FHN+D+D  AN+ ASNL+SRDPDL  
Sbjct: 121 HSS-HVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSK 180

Query: 181 RMVFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISS 234
           RM+FHT D++  TE L QY+VVFLAALVGM+KE K+K I+HL ++M+ GA LM+ S
Sbjct: 181 RMIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRS 234

[Close]

BLAST of Chy8G156810 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 257.7 bits (657), Expect = 1.2e-68
Identity = 129/234 (55.13%), Postives = 177/234 (75.64%), Query Frame = 0


Query: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           M C++E L+Q +C+LY++IS LESLKP  +V+ LF +LV TC+ P +P+  +  +   +Q
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCI-PPNPNIDVTKMCDRVQ 60

Query: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKF-DSPIDHLHVFPYFSNYIKLSLLEFSILRSHG 120
           ++R NLI++CG AE  LE HFS +L  + D+P+ HL++FPY++NY+KL  LEF +L  + 
Sbjct: 61  EIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNL 120

Query: 121 PLALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTRM 180
              +P +VAF+GSGPLPLTS+VLA+ HL  TIFHN+D+DP ANS AS L+S DPD+  RM
Sbjct: 121 NGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRM 180

Query: 181 VFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISS 234
            FHT DIM VTE LK ++VVFLAALVGM KEEK+KVI+HL ++M+ GA LM+ S
Sbjct: 181 FFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRS 233

[Close]

BLAST of Chy8G156810 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 250.0 bits (637), Expect = 2.5e-66
Identity = 125/235 (53.19%), Postives = 176/235 (74.89%), Query Frame = 0


Query: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSL-SKPL 60
           M CE   +++++ +LY++IS+LESLKP  NVDTLF +LV TC+ PT  +  +  +  + +
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL-PTDTNIDVTEIHDEKV 60

Query: 61  QQMRSNLIRLCGQAESLLEFHFSDLLAKF-DSPIDHLHVFPYFSNYIKLSLLEFSILRSH 120
           + MRS+LI+LCG+AE  LE HFS +L  F D+P++HLH+FPY++NY+KL  LEF +L  H
Sbjct: 61  KDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQH 120

Query: 121 GPLALPSAVAFVGSGPLPLTSVVLATRHLTSTIFHNYDLDPVANSKASNLMSRDPDLKTR 180
               +P+ VAF+GSGP+PLTS+VLA  HL +T FHN+D+D  AN+ ASNL+SRD DL  R
Sbjct: 121 -TTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKR 180

Query: 181 MVFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMISS 234
           M+FHT D++   E L QY+VVFLAALVGM+KE K+K I+HL ++M+ GA +M+ S
Sbjct: 181 MIFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRS 233

The following BLAST results are available for this feature:

BLAST of Chy8G156810 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7X5	8.8e-72	56.90	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
Q9XGI7	1.8e-69	56.84	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
Q9FF79	7.7e-68	53.81	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
O80483	1.7e-67	55.13	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9FKT9	3.6e-65	53.19	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]

BLAST of Chy8G156810 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVX1	4.9e-126	75.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G561410 PE=3 SV=1	[more]
A0A1S3BPH1	1.1e-125	73.37	nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103492113 PE=3 SV=1	[more]
A0A6J1JPC1	6.0e-100	64.98	nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111486493 PE=3 SV=...	[more]
A0A6J1EU11	1.0e-99	64.87	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111437742 PE=3 S...	[more]
A0A5A7U6Y1	2.2e-89	67.87	Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]

BLAST of Chy8G156810 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004135580.1	1.31e-161	75.45	nicotianamine synthase [Cucumis sativus] >KGN66010.1 hypothetical protein Csa_00...	[more]
XP_008450545.1	4.97e-161	73.37	PREDICTED: nicotianamine synthase-like [Cucumis melo]	[more]
XP_038878580.1	1.51e-143	91.42	probable nicotianamine synthase 4 [Benincasa hispida]	[more]
XP_022989439.1	1.12e-127	64.98	nicotianamine synthase-like [Cucurbita maxima]	[more]
XP_022931567.1	2.17e-127	64.87	nicotianamine synthase-like [Cucurbita moschata]	[more]

BLAST of Chy8G156810 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	6.2e-73	56.90	nicotianamine synthase 4	[more]
AT5G04950.1	5.5e-69	53.81	nicotianamine synthase 1	[more]
AT1G09240.1	1.2e-68	55.13	nicotianamine synthase 3	[more]
AT5G56080.1	2.5e-66	53.19	nicotianamine synthase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 5..233 e-value: 1.6E-92 score: 309.8
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 4..235
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 1..304 score: 44.650684
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 6..238 e-value: 1.6E-39 score: 137.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy8G156810.1	Chy8G156810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity