Chy7G137540 (gene) Cucumber (hystrix) v1

Overview
NameChy7G137540
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationchrH07: 13185641 .. 13186750 (+)
RNA-Seq ExpressionChy7G137540
SyntenyChy7G137540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCCCTCACAGGACAATCCCAACCACGCCAAACCACCATTCTCATCTAATCTATTGCAAGAAACTCAAAAGAATGGCCGCTATTTCCATCGATCCAAGGCTCAAACCCCAAATACCAAAGCTCCGTATTCAAAATGAAGCCACAGCAGAAGAGATTGAAGAGGTAATTCGAGTTTATGGAGATGGGCGTGTTGAAAGGCCCCCAATCATCCCAAATGTATCCTGCGGCATCACAGCATCAGACAAACGCGTAATTGCAAAAGATGTTCTCATAGAAAATTTCACAAACACCTGGGCGCGGATTTACGTAGCTAACACTCAACGAAACCTCCCCTTACTTGTTTATTTCCATGGCGGTGGCTTCTGTGTTGGCTCTGCCGCATGGAGCTGTTACCATGAATTCCTCCTCGACCTTGCTTCCAAGGCAAAATGTGTAATACTCTCAGTTAACTATCGTCTTTCCCCTGAAAATCGCCTCCCCGCCGCTTACGAAGACGGATTCAACACACTCATGTGGTTGAAACAACAAATAATAACCAACCCAATTGAACACAAATGGTGGTTAGGCAATTCCAATGTATCAAAACTATTCCTCGTCGGCGATAGCGCCGGAGCAAACATAGCATACAACATCACCACACGGCTAGGGCTCAACGAACCCACAATCCTAAAACCCCTAACCCTAAAGGGCATAATCCTGATTCAACCTTTCTTTGGCGGAGAATCACGAACCAAATCAGAAAAGCTCACCGCTCAGCCATCTTCCGGTTCCGCATTAACCCTCGACGCCGCCGACACATACTGGCGTCTGTCCCTGCCAGCGGGGGCCAATCGCGACCATACATGGAGCAATCCGCTGGTGAATGGCGTGGCAAAGTTGAGAGAAACAAAAGTGCCACCGTTAATGGTGTGCATATCGGAGTTGGACATACTGGTGGACAGAAACTTGGAGTTCTGTGCAGCGATGGCGGCGGCCAAGAAGAAAGTGGAGAGAGTAATATTGAAAGGAGTTGGACATAGCTTTCAAATTTTGAAGAAAGACCAGATTTCTAATGTGCGAAGACAAGAAATGATGGGTTGTATTCAGGCTTTTATTCAAATTCAACAATAG

mRNA sequence

ACCCCTCACAGGACAATCCCAACCACGCCAAACCACCATTCTCATCTAATCTATTGCAAGAAACTCAAAAGAATGGCCGCTATTTCCATCGATCCAAGGCTCAAACCCCAAATACCAAAGCTCCGTATTCAAAATGAAGCCACAGCAGAAGAGATTGAAGAGGTAATTCGAGTTTATGGAGATGGGCGTGTTGAAAGGCCCCCAATCATCCCAAATGTATCCTGCGGCATCACAGCATCAGACAAACGCGTAATTGCAAAAGATGTTCTCATAGAAAATTTCACAAACACCTGGGCGCGGATTTACGTAGCTAACACTCAACGAAACCTCCCCTTACTTGTTTATTTCCATGGCGGTGGCTTCTGTGTTGGCTCTGCCGCATGGAGCTGTTACCATGAATTCCTCCTCGACCTTGCTTCCAAGGCAAAATGTGTAATACTCTCAGTTAACTATCGTCTTTCCCCTGAAAATCGCCTCCCCGCCGCTTACGAAGACGGATTCAACACACTCATGTGGTTGAAACAACAAATAATAACCAACCCAATTGAACACAAATGGTGGTTAGGCAATTCCAATGTATCAAAACTATTCCTCGTCGGCGATAGCGCCGGAGCAAACATAGCATACAACATCACCACACGGCTAGGGCTCAACGAACCCACAATCCTAAAACCCCTAACCCTAAAGGGCATAATCCTGATTCAACCTTTCTTTGGCGGAGAATCACGAACCAAATCAGAAAAGCTCACCGCTCAGCCATCTTCCGGTTCCGCATTAACCCTCGACGCCGCCGACACATACTGGCGTCTGTCCCTGCCAGCGGGGGCCAATCGCGACCATACATGGAGCAATCCGCTGGTGAATGGCGTGGCAAAGTTGAGAGAAACAAAAGTGCCACCGTTAATGGTGTGCATATCGGAGTTGGACATACTGGTGGACAGAAACTTGGAGTTCTGTGCAGCGATGGCGGCGGCCAAGAAGAAAGTGGAGAGAGTAATATTGAAAGGAGTTGGACATAGCTTTCAAATTTTGAAGAAAGACCAGATTTCTAATGTGCGAAGACAAGAAATGATGGGTTGTATTCAGGCTTTTATTCAAATTCAACAATAG

Coding sequence (CDS)

ACCCCTCACAGGACAATCCCAACCACGCCAAACCACCATTCTCATCTAATCTATTGCAAGAAACTCAAAAGAATGGCCGCTATTTCCATCGATCCAAGGCTCAAACCCCAAATACCAAAGCTCCGTATTCAAAATGAAGCCACAGCAGAAGAGATTGAAGAGGTAATTCGAGTTTATGGAGATGGGCGTGTTGAAAGGCCCCCAATCATCCCAAATGTATCCTGCGGCATCACAGCATCAGACAAACGCGTAATTGCAAAAGATGTTCTCATAGAAAATTTCACAAACACCTGGGCGCGGATTTACGTAGCTAACACTCAACGAAACCTCCCCTTACTTGTTTATTTCCATGGCGGTGGCTTCTGTGTTGGCTCTGCCGCATGGAGCTGTTACCATGAATTCCTCCTCGACCTTGCTTCCAAGGCAAAATGTGTAATACTCTCAGTTAACTATCGTCTTTCCCCTGAAAATCGCCTCCCCGCCGCTTACGAAGACGGATTCAACACACTCATGTGGTTGAAACAACAAATAATAACCAACCCAATTGAACACAAATGGTGGTTAGGCAATTCCAATGTATCAAAACTATTCCTCGTCGGCGATAGCGCCGGAGCAAACATAGCATACAACATCACCACACGGCTAGGGCTCAACGAACCCACAATCCTAAAACCCCTAACCCTAAAGGGCATAATCCTGATTCAACCTTTCTTTGGCGGAGAATCACGAACCAAATCAGAAAAGCTCACCGCTCAGCCATCTTCCGGTTCCGCATTAACCCTCGACGCCGCCGACACATACTGGCGTCTGTCCCTGCCAGCGGGGGCCAATCGCGACCATACATGGAGCAATCCGCTGGTGAATGGCGTGGCAAAGTTGAGAGAAACAAAAGTGCCACCGTTAATGGTGTGCATATCGGAGTTGGACATACTGGTGGACAGAAACTTGGAGTTCTGTGCAGCGATGGCGGCGGCCAAGAAGAAAGTGGAGAGAGTAATATTGAAAGGAGTTGGACATAGCTTTCAAATTTTGAAGAAAGACCAGATTTCTAATGTGCGAAGACAAGAAATGATGGGTTGTATTCAGGCTTTTATTCAAATTCAACAATAG

Protein sequence

TPHRTIPTTPNHHSHLIYCKKLKRMAAISIDPRLKPQIPKLRIQNEATAEEIEEVIRVYGDGRVERPPIIPNVSCGITASDKRVIAKDVLIENFTNTWARIYVANTQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYEDGFNTLMWLKQQIITNPIEHKWWLGNSNVSKLFLVGDSAGANIAYNITTRLGLNEPTILKPLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALTLDAADTYWRLSLPAGANRDHTWSNPLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCAAMAAAKKKVERVILKGVGHSFQILKKDQISNVRRQEMMGCIQAFIQIQQ*
Homology
BLAST of Chy7G137540 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 2.5e-86
Identity = 171/321 (53.27%), Postives = 213/321 (66.36%), Query Frame = 0

Query: 50  EEIEEVIRVYGDGRVERPPIIPNVSCGITASDKRVIAKDVLIENFTNTWARIYV-----A 109
           EEIE +I+V+ DG VERPPI+P VS  I  S K   A D+ + N  +TW R+Y+     A
Sbjct: 30  EEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSK-ATAFDIKLSN--DTWTRVYIPDAAAA 89

Query: 110 NTQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYE 169
           +    LPLLVYFHGGGFCVGSAAWSCYH+FL  LA KA+CVI+SVNYRL+PE+RLPAAY+
Sbjct: 90  SPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPEHRLPAAYD 149

Query: 170 DGFNTLMWLKQQIITNPIEHKWWLGNSNVSKLFLVGDSAGANIAYNITTRLGLNEPTILK 229
           DG N + WL +Q I+    +  WL   N+S +FL GDSAGANIAY +  R+ +       
Sbjct: 150 DGVNVVSWLVKQQISTGGGYPSWLSKCNLSNVFLAGDSAGANIAYQVAVRI-MASGKYAN 209

Query: 230 PLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALTLDAADTYWRLSLPAGANRDHTWSN 289
            L LKGIILI PFFGGESRT SEK      S SALTL A+D YWRL+LP GA+RDH W N
Sbjct: 210 TLHLKGIILIHPFFGGESRTSSEKQQHHTKS-SALTLSASDAYWRLALPRGASRDHPWCN 269

Query: 290 PLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCAAMAAAKKKVERVILKGVGHSFQIL 349
           PL++        K+P  MV ++E DIL +RNLE C  M +  K+VE ++  GVGH+F IL
Sbjct: 270 PLMSSAG----AKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGGVGHAFHIL 329

Query: 350 KKDQISNVRRQEMMGCIQAFI 366
               +S  R  +MM  +  FI
Sbjct: 330 DNSSVSRDRIHDMMCRLHNFI 341

BLAST of Chy7G137540 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.2e-72
Identity = 142/321 (44.24%), Postives = 204/321 (63.55%), Query Frame = 0

Query: 50  EEIEEVIRVYGDGRVERPPIIPNVSCGITASDKRVIAKDVLIENFTNTWARIYVANTQ-- 109
           +E+E +I+VY DG VER  ++P V   +   +  V   DV+I+  TN WAR+YV  T   
Sbjct: 25  DEVEGLIKVYKDGHVERSQLLPCVDPSLPL-ELGVTCSDVVIDKLTNVWARLYVPMTTTK 84

Query: 110 ---RNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYE 169
                LPL+VYFHGGGFCVGSA+W CYHEFL  L+++++C+++SVNYRL+PEN LPAAYE
Sbjct: 85  SSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPLPAAYE 144

Query: 170 DGFNTLMWLKQQIITNPIEHKWWLGNSNVSKLFLVGDSAGANIAYNITTRLGLNEPTILK 229
           DG N ++WL +    N      W    +  ++FL GDSAG NIA  +  RL   E   LK
Sbjct: 145 DGVNAILWLNKARNDN-----LWAKQCDFGRIFLAGDSAGGNIAQQVAARLASPEDLALK 204

Query: 230 PLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALTLDAADTYWRLSLPAGANRDHTWSN 289
              ++G ILIQPF+ GE RT+SE+      + + LTL ++D +WR+SLP GANR+H +  
Sbjct: 205 ---IEGTILIQPFYSGEERTESERRVGNDKT-AVLTLASSDAWWRMSLPRGANREHPYCK 264

Query: 290 PLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCAAMAAAKKKVERVILKGVGHSFQIL 349
           P+      ++ + V   +VC++E+D+L+D N+E C      +  ++RV+ KGVGH+F IL
Sbjct: 265 PV---KMIIKSSTVTRTLVCVAEMDLLMDSNMEMC---DGNEDVIKRVLHKGVGHAFHIL 324

Query: 350 KKDQISNVRRQEMMGCIQAFI 366
            K Q+++    EM+  I AFI
Sbjct: 325 GKSQLAHTTTLEMLCQIDAFI 329

BLAST of Chy7G137540 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.6e-46
Identity = 107/300 (35.67%), Postives = 170/300 (56.67%), Query Frame = 0

Query: 49  AEEIEEVIRVYGDGRVERPPIIPNVSCGIT-ASDKRVIAKDVLIENFTNTWARIY---VA 108
           AE+   ++++  +G V R   I  ++  I   +++ V+ KD +     N   R+Y    A
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 109 NTQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYE 168
           + +  LP++V+FHGGGFC GS +W  +H F L LAS    +++S +YRL+PE+RLPAA+E
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 169 DGFNTLMWLKQQIITNPIEHKWWLGNSNV--SKLFLVGDSAGANIAYNITTRLGLNEPTI 228
           D    L WL  Q +++ + H W+   ++V   ++F+VGDS+G NIA+ +  R G +    
Sbjct: 131 DAEAVLTWLWDQAVSDGVNH-WFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIE 190

Query: 229 LKPLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALTLDAADTYWRLSLPAGANRDHTW 288
           L P+ ++G +L+ PFFGGE RT SE       S + L+LD  D +WRLSLP GA RDH  
Sbjct: 191 LTPVRVRGYVLMGPFFGGEERTNSE----NGPSEALLSLDLLDKFWRLSLPNGATRDHHM 250

Query: 289 SNPLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCAAM-AAAKKKVERVILKGVGHSF 342
           +NP       L    + P++V +   ++L DR  E+   +     K+V+ +  +   H F
Sbjct: 251 ANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of Chy7G137540 vs. ExPASy Swiss-Prot
Match: Q0ZPV7 (Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.4e-39
Identity = 106/287 (36.93%), Postives = 148/287 (51.57%), Query Frame = 0

Query: 61  DGRVERPPIIPNVSCG-ITASDKRVIAKDVLIENFTNTWARIYVAN----TQRNLPLLVY 120
           D  + RP  IP+ +      S   V+ KD+ +    NT+ R+++          LPL+VY
Sbjct: 29  DRTITRPIQIPSTAASPDPTSSSPVLTKDLALNPLHNTFVRLFLPRHALYNSAKLPLVVY 88

Query: 121 FHGGGFCVGSAAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYEDGFNTLMWLKQ 180
           FHGGGF + SAA + +H+F  ++A  A  VI SV+YRL+PE+RLPAAY+D    L W+K 
Sbjct: 89  FHGGGFILFSAASTIFHDFCCEMAVHAGVVIASVDYRLAPEHRLPAAYDDAMEALQWIKD 148

Query: 181 QIITNPIEHKWWLGNSNVSKLFLVGDSAGANIAYNITTRLGLNEPTILKPLTLKGIILIQ 240
                    +W    ++ S  F++G+SAG NIAY+   R       +L PL +KG++L +
Sbjct: 149 S------RDEWLTNFADFSNCFIMGESAGGNIAYHAGLRAAAVADELL-PLKIKGLVLDE 208

Query: 241 PFFGGESRTKSEKLTAQPSSGSALTLDAADTYWRLSLPAGANRDHTWSNPLVNGVAKLRE 300
           P FGG  RT SE   A   + S L     D  W LSLP GA+RDH + NP          
Sbjct: 209 PGFGGSKRTGSELRLA---NDSRLPTFVLDLIWELSLPMGADRDHEYCNPTAESEPLYSF 268

Query: 301 TKVPPL----MVCISELDILVDRNLEFCAAMAAAKKKVERVILKGVG 339
            K+  L    MV     D ++DR +E   A    KK V+ V    VG
Sbjct: 269 DKIRSLGWRVMVVGCHGDPMIDRQMEL--AERLEKKGVDVVAQFDVG 303

BLAST of Chy7G137540 vs. ExPASy Swiss-Prot
Match: Q9LVB8 (Probable carboxylesterase 120 OS=Arabidopsis thaliana OX=3702 GN=CXE20 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.6e-38
Identity = 103/296 (34.80%), Postives = 157/296 (53.04%), Query Frame = 0

Query: 61  DGRVERPPIIPNVSCGITASD----KRVIAKDVLIENFTNTWARIYVANTQRN------- 120
           DG + R   + N  C     D       ++KD+ +    +TW R+Y+ ++  N       
Sbjct: 21  DGSITRD--LSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQ 80

Query: 121 -LPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYEDGFN 180
            LP++VY+HGGGF + S     +H+F  ++A     +++S +YRL+PE+RLPAAY+DG  
Sbjct: 81  KLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVE 140

Query: 181 TLMWLKQQIITNPIEHKWWLGNSNVSKLFLVGDSAGANIAYNITTRLGLNEPTILKPLTL 240
            L W+K        + +W   +++ S +FL+G SAG N+AYN+  R  ++  + L PL +
Sbjct: 141 ALDWIKTS------DDEWIKSHADFSNVFLMGTSAGGNLAYNVGLR-SVDSVSDLSPLQI 200

Query: 241 KGIILIQPFFGGESRTKSEKLTAQPSSGSALTLDAADTYWRLSLPAGANRDHTWSNPLV- 300
           +G+IL  PFFGGE R++SE    +  +         D  W LSLP G +RDH +SNP V 
Sbjct: 201 RGLILHHPFFGGEERSESE---IRLMNDQVCPPIVTDVMWDLSLPVGVDRDHEYSNPTVG 260

Query: 301 NGVAKLRETKVPPL----MVCISELDILVDRNLEFCAAMAAAKKKVERVILKGVGH 340
           +G  KL   K+  L    M+   E D ++D  L+   A    KK VE V     GH
Sbjct: 261 DGSEKLE--KIGRLRWKVMMIGGEDDPMID--LQKDVAKLMKKKGVEVVEHYTGGH 300

BLAST of Chy7G137540 vs. ExPASy TrEMBL
Match: A0A6J1G8X8 (probable carboxylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111452001 PE=4 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 6.2e-160
Identity = 282/346 (81.50%), Postives = 302/346 (87.28%), Query Frame = 0

Query: 23  KRMAAISIDPRLKPQIPKLRIQNEATAEEIEEVIRVYGDGRVERPPIIPNVSCGITASDK 82
           KRMAA+ IDPRLK Q+ K  +QN  T+EEIE +IRVY DG VERPPIIPNV+C  TASDK
Sbjct: 4   KRMAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDK 63

Query: 83  RVIAKDVLIENFTNTWARIYVANTQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKA 142
            V AKDVLI+ FTNTWARIY+ N+QRNLPLLVYFHGGGFCVGSAAWSCYHEFLL+LASKA
Sbjct: 64  GVTAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKA 123

Query: 143 KCVILSVNYRLSPENRLPAAYEDGFNTLMWLKQQIITNPIEHKWWLGNSNVSKLFLVGDS 202
           KCVILS+NYRLSPENRLPAAY+DGFN LMWLKQQ ITNPIEHKWWLGN N SKLFL GDS
Sbjct: 124 KCVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDS 183

Query: 203 AGANIAYNITTRLGLNEPTILKPLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALTLD 262
           AGANI+YN  TR+G NE T+LKPLTLKGIILIQPFFGGESRTKSEKL A PSS SALTL 
Sbjct: 184 AGANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLS 243

Query: 263 AADTYWRLSLPAGANRDHTWSNPLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCAAM 322
           AADTYWRLSLP  ANRDH WSNPLVNG AKL+E K+PPLMVCISELDIL+DRNLEFCAAM
Sbjct: 244 AADTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAM 303

Query: 323 AAAKKKVERVILKGVGHSFQILKKDQISNVRRQEMMGCIQAFIQIQ 369
           A A KKVERVILKGVGH FQIL KDQIS  R QE+MG I+AFI  Q
Sbjct: 304 AGAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 349

BLAST of Chy7G137540 vs. ExPASy TrEMBL
Match: A0A6J1DL90 (probable carboxylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111022166 PE=4 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 4.0e-159
Identity = 283/345 (82.03%), Postives = 299/345 (86.67%), Query Frame = 0

Query: 21  KLKRMAAISIDPRLKPQIPKLRIQNEATAEEIEEVIRVYGDGRVERPPIIPNVSCGITAS 80
           K KRMAAISIDPRLK Q+PK  +Q  +TAEEIE +IRVY DG VERPPIIPNV C  TAS
Sbjct: 2   KFKRMAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTAS 61

Query: 81  DKRVIAKDVLIENFTNTWARIYVANTQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLAS 140
           +K V AKDVLI+NFTNTWARIY+AN+QR LPLLVYFHGGGFCVGSAAWSCYHEFLLDLAS
Sbjct: 62  NKGVSAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLAS 121

Query: 141 KAKCVILSVNYRLSPENRLPAAYEDGFNTLMWLKQQIITNPIEHKWWLGNSNVSKLFLVG 200
           KAKC ILS+NYRLSPENRLPAAYEDGFN LMWLKQQ I+ P EHKWWLGN N S LFL G
Sbjct: 122 KAKCAILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAG 181

Query: 201 DSAGANIAYNITTRLGLNEPTILKPLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALT 260
           DSAGANIAYNI TR GLNEP  LKPLTLKG+ILIQPFFGGESRTKSEKL AQPS+ SALT
Sbjct: 182 DSAGANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPST-SALT 241

Query: 261 LDAADTYWRLSLPAGANRDHTWSNPLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCA 320
           L  ADTYWRLSLP GA+RDH WSNPLVNG  KLR+TK+PP MVCISELDILVDRNLEFCA
Sbjct: 242 LATADTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCA 301

Query: 321 AMAAAKKKVERVILKGVGHSFQILKKDQISNVRRQEMMGCIQAFI 366
           AMAAA KKVERVI KGVGH FQIL KDQIS +R QEMM  IQ FI
Sbjct: 302 AMAAAGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFI 345

BLAST of Chy7G137540 vs. ExPASy TrEMBL
Match: A0A6J1KCZ3 (probable carboxylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111493192 PE=4 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.5e-158
Identity = 281/344 (81.69%), Postives = 301/344 (87.50%), Query Frame = 0

Query: 25  MAAISIDPRLKPQIPKLRIQNEATAEEIEEVIRVYGDGRVERPPIIPNVSCGITASDKRV 84
           MAA+ IDPRLK Q+ K  +QN AT+EEIE +IRVY DG VERPPIIPNV+C  TAS+K V
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60

Query: 85  IAKDVLIENFTNTWARIYVANTQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 144
            AKDVLI+ FTNTWARIY+ N+QRNLPLLVYFHGGGFCVGSAAWSCYHEFLL+LASKAKC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 145 VILSVNYRLSPENRLPAAYEDGFNTLMWLKQQIITNPIEHKWWLGNSNVSKLFLVGDSAG 204
           VILS+NYRLSPENRLPAAYEDGFN +MWLKQQ ITNPIEHKWWLGN N SKLFL GDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 205 ANIAYNITTRLGLNEPTILKPLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALTLDAA 264
           ANIAYN  TRLG NE T+LKP TLKGIILIQPFFGGESRTKSEKL A PSS SALTL A+
Sbjct: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 265 DTYWRLSLPAGANRDHTWSNPLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCAAMAA 324
           DTYWRLSLP  ANRDH WSNPLVNG AKL+E K+PPLMVCISELDIL+DRNLEFCAAMAA
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300

Query: 325 AKKKVERVILKGVGHSFQILKKDQISNVRRQEMMGCIQAFIQIQ 369
           A KKVERVILKGVGH FQIL KDQIS  R QE+MG I+AFI  Q
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 344

BLAST of Chy7G137540 vs. ExPASy TrEMBL
Match: A0A2N9EQ59 (Abhydrolase_3 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4850 PE=4 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 1.6e-120
Identity = 221/344 (64.24%), Postives = 264/344 (76.74%), Query Frame = 0

Query: 25  MAAISIDPRLKPQIPKLRIQNEATAEEIEEVIRVYGDGRVERPPIIPNVSCGITASDKRV 84
           MAAIS+DPR K Q+ K   Q+    EEIE +I+V  DG VERPPI+P V C + A ++ V
Sbjct: 1   MAAISLDPRFKLQVGKNHHQHGGVVEEIEGLIKVCMDGHVERPPIVPIVPCTM-ALERGV 60

Query: 85  IAKDVLIENFTNTWARIYVANTQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 144
            A DV+I+  T+ WARIYV +    LPLLVYFHGGGFCVGSAAWSCY+EFL +LASKA C
Sbjct: 61  TANDVVIDKCTSLWARIYVPSCSGKLPLLVYFHGGGFCVGSAAWSCYNEFLSNLASKANC 120

Query: 145 VILSVNYRLSPENRLPAAYEDGFNTLMWLKQQIITNPIEHKWWLGNSNVSKLFLVGDSAG 204
           VI+SVNYRL+PENRLPAAYEDGFNTLMW+KQQ +    EHKWWL + N++ +FL GDS+G
Sbjct: 121 VIISVNYRLAPENRLPAAYEDGFNTLMWVKQQALNGSNEHKWWLRHCNLTSMFLGGDSSG 180

Query: 205 ANIAYNITTRL---GLNEPTILKPLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALTL 264
           ANIAYN+ TRL   G +E TI++PL LKG ILIQPFFGGE+RT SEK T QP++ S LTL
Sbjct: 181 ANIAYNVATRLGSSGTSESTIIRPLCLKGTILIQPFFGGEARTWSEKYTNQPAN-SGLTL 240

Query: 265 DAADTYWRLSLPAGANRDHTWSNPLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCAA 324
             +DTYWRLSLP G NRD+ W NPL NG+AKLRE ++P +MVC+SE DIL DRNLEFC A
Sbjct: 241 LVSDTYWRLSLPIGTNRDNPWCNPLANGMAKLREFRLPSIMVCVSETDILKDRNLEFCTA 300

Query: 325 MAAAKKKVERVILKGVGHSFQILKKDQISNVRRQEMMGCIQAFI 366
           +  A K+VE VI KGVGH+FQIL   Q+S  R  EMM  I+AFI
Sbjct: 301 LTNAGKRVETVIYKGVGHAFQILHNSQLSQPRTHEMMAHIKAFI 342

BLAST of Chy7G137540 vs. ExPASy TrEMBL
Match: A0A2R6RU97 (Carboxylesterase OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc04005 PE=4 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 6.2e-120
Identity = 218/345 (63.19%), Postives = 262/345 (75.94%), Query Frame = 0

Query: 25  MAAISIDPRLKPQIPKLRIQNEATAEEIEEVIRVYGDGRVERPPIIPNVSCGITASDKRV 84
           MA IS+DPRL  Q  K   QN A  EEIE +IRVY DG VER  ++PNVSC +  S+  V
Sbjct: 1   MATISLDPRLNLQTGKTNHQNSALVEEIEGIIRVYKDGSVERHLVLPNVSCAVN-SELSV 60

Query: 85  IAKDVLIENFTNTWARIYVANTQ-RNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAK 144
            +KDV+I+ FT+ WAR+YV + Q   LPLLVYFHGGGFCVGS +WSCYH FL +LAS A 
Sbjct: 61  TSKDVVIDKFTSLWARLYVPSRQYGKLPLLVYFHGGGFCVGSTSWSCYHNFLSNLASIAG 120

Query: 145 CVILSVNYRLSPENRLPAAYEDGFNTLMWLKQQIITNPIEHKWWLGNSNVSKLFLVGDSA 204
           CVI+SVNYRL+PENRLPAAYEDGF  +MW+KQQ +    E KWWL   + S +FL GDSA
Sbjct: 121 CVIVSVNYRLAPENRLPAAYEDGFKAVMWVKQQALNRSDEEKWWLSRCSFSSVFLAGDSA 180

Query: 205 GANIAYNITTRLGLN---EPTILKPLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALT 264
           GANIA+N+ TRLG N   +P+ILKPL LKG ILIQPFFGGE RT+SE+ T QP  GSALT
Sbjct: 181 GANIAHNVATRLGSNGTSDPSILKPLCLKGTILIQPFFGGEMRTESERQTVQP-PGSALT 240

Query: 265 LDAADTYWRLSLPAGANRDHTWSNPLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCA 324
           L ++DTYW+LSLP GANRDH W NPL  G  K+ +  +PP+MVCISE+DIL DRNLEFCA
Sbjct: 241 LPSSDTYWQLSLPVGANRDHHWCNPLAKGATKVGDYGLPPMMVCISEMDILKDRNLEFCA 300

Query: 325 AMAAAKKKVERVILKGVGHSFQILKKDQISNVRRQEMMGCIQAFI 366
           AMA+A K+VE+VI +GVGH+FQIL    +S +R  EMM  ++AFI
Sbjct: 301 AMASAGKRVEKVIYRGVGHAFQILHNSHLSQLRTHEMMSHLKAFI 343

BLAST of Chy7G137540 vs. NCBI nr
Match: XP_038904086.1 (probable carboxylesterase 17 [Benincasa hispida])

HSP 1 Score: 633 bits (1632), Expect = 3.60e-227
Identity = 314/358 (87.71%), Postives = 322/358 (89.94%), Query Frame = 0

Query: 10  PNHHSHLIYCKKLKRMAAISIDPRLKPQIPKLRIQNEATAEEIEEVIRVYGDGRVERPPI 69
           P HHSHLIY  K KRMA ISIDPRLK Q+PKL IQNEAT EEIE +IRVY DG VER PI
Sbjct: 2   PEHHSHLIYYMKFKRMATISIDPRLKLQMPKLHIQNEATVEEIEGLIRVYADGHVERFPI 61

Query: 70  IPNVSCGITASDKRVIAKDVLIENFTNTWARIYVANTQRNLPLLVYFHGGGFCVGSAAWS 129
           IPNVSC  TASDK V AKDVLI+NFTNTWARIYVAN+QRNLPLLVYFHGGGFCVGSAAWS
Sbjct: 62  IPNVSCNTTASDKGVTAKDVLIDNFTNTWARIYVANSQRNLPLLVYFHGGGFCVGSAAWS 121

Query: 130 CYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYEDGFNTLMWLKQQIITNPIEHKWWLG 189
           CYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYEDGFNTLMWLKQ  ITNPIEHKWWLG
Sbjct: 122 CYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYEDGFNTLMWLKQLAITNPIEHKWWLG 181

Query: 190 NSNVSKLFLVGDSAGANIAYNITTRLGLNEPTILKPLTLKGIILIQPFFGGESRTKSEKL 249
           N N SKLFL GDSAGANIAYNI  R+GLNEPTILKPL LKGIILIQPFFGGESRTKSEKL
Sbjct: 182 NCNQSKLFLAGDSAGANIAYNIAARVGLNEPTILKPLNLKGIILIQPFFGGESRTKSEKL 241

Query: 250 TAQPSSGSALTLDAADTYWRLSLPAGANRDHTWSNPLVNGVAKLRETKVPPLMVCISELD 309
             QPSS SALTLDAADTYWRLSLP GANRDHTWSNPLVNG  KLRETKVPPLMVCISELD
Sbjct: 242 ATQPSSASALTLDAADTYWRLSLPPGANRDHTWSNPLVNGGVKLRETKVPPLMVCISELD 301

Query: 310 ILVDRNLEFCAAMAAAKKKVERVILKGVGHSFQILKKDQISNVRRQEMMGCIQAFIQI 367
           ILVDRNLEFCAAM AA KKVERVILKGVGHSFQIL KDQIS+ RR+EMM CIQ FIQ+
Sbjct: 302 ILVDRNLEFCAAMTAAGKKVERVILKGVGHSFQILNKDQISSARRREMMVCIQRFIQL 359

BLAST of Chy7G137540 vs. NCBI nr
Match: XP_022948283.1 (probable carboxylesterase 17 [Cucurbita moschata])

HSP 1 Score: 570 bits (1469), Expect = 1.66e-202
Identity = 281/343 (81.92%), Postives = 301/343 (87.76%), Query Frame = 0

Query: 23  KRMAAISIDPRLKPQIPKLRIQNEATAEEIEEVIRVYGDGRVERPPIIPNVSCGITASDK 82
           KRMAA+ IDPRLK Q+ K  +QN  T+EEIE +IRVY DG VERPPIIPNV+C  TASDK
Sbjct: 4   KRMAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDK 63

Query: 83  RVIAKDVLIENFTNTWARIYVANTQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKA 142
            V AKDVLI+ FTNTWARIY+ N+QRNLPLLVYFHGGGFCVGSAAWSCYHEFLL+LASKA
Sbjct: 64  GVTAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKA 123

Query: 143 KCVILSVNYRLSPENRLPAAYEDGFNTLMWLKQQIITNPIEHKWWLGNSNVSKLFLVGDS 202
           KCVILS+NYRLSPENRLPAAY+DGFN LMWLKQQ ITNPIEHKWWLGN N SKLFL GDS
Sbjct: 124 KCVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDS 183

Query: 203 AGANIAYNITTRLGLNEPTILKPLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALTLD 262
           AGANI+YN  TR+G NE T+LKPLTLKGIILIQPFFGGESRTKSEKL A PSS SALTL 
Sbjct: 184 AGANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLS 243

Query: 263 AADTYWRLSLPAGANRDHTWSNPLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCAAM 322
           AADTYWRLSLP  ANRDH WSNPLVNG AKL+E K+PPLMVCISELDIL+DRNLEFCAAM
Sbjct: 244 AADTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAM 303

Query: 323 AAAKKKVERVILKGVGHSFQILKKDQISNVRRQEMMGCIQAFI 365
           A A KKVERVILKGVGH FQIL KDQIS  R QE+MG I+AFI
Sbjct: 304 AGAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFI 346

BLAST of Chy7G137540 vs. NCBI nr
Match: XP_023523365.1 (probable carboxylesterase 17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 568 bits (1463), Expect = 1.36e-201
Identity = 279/343 (81.34%), Postives = 300/343 (87.46%), Query Frame = 0

Query: 23  KRMAAISIDPRLKPQIPKLRIQNEATAEEIEEVIRVYGDGRVERPPIIPNVSCGITASDK 82
           KRMAA+ +DPRLK Q+ K  +QN  T+EEIE +IRVY DG VERPPIIPNV+C  TASDK
Sbjct: 4   KRMAAMYVDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDK 63

Query: 83  RVIAKDVLIENFTNTWARIYVANTQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKA 142
            V AKDVLI+ FTNTWARIY+ N+QRNLPLLVYFHGGGFCVGSAAWSCYHEFLL+LASKA
Sbjct: 64  GVTAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKA 123

Query: 143 KCVILSVNYRLSPENRLPAAYEDGFNTLMWLKQQIITNPIEHKWWLGNSNVSKLFLVGDS 202
           KCVILS+NYRLSPENRLPAAY+DGFN LMWLKQQ ITNPIEHKWW GN N SKLFL GDS
Sbjct: 124 KCVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWSGNCNPSKLFLAGDS 183

Query: 203 AGANIAYNITTRLGLNEPTILKPLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALTLD 262
           AGANI YN  TRLG NE T+LKPLTLKGIILIQPFFGGESRTKSEKL A PSS SALTL 
Sbjct: 184 AGANIVYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLS 243

Query: 263 AADTYWRLSLPAGANRDHTWSNPLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCAAM 322
           A+DTYWRLSLP  ANRDH WSNPLVNG AKL+ETK+PPLMVCISELDIL+DRNLEFCAAM
Sbjct: 244 ASDTYWRLSLPPTANRDHPWSNPLVNGGAKLKETKLPPLMVCISELDILLDRNLEFCAAM 303

Query: 323 AAAKKKVERVILKGVGHSFQILKKDQISNVRRQEMMGCIQAFI 365
           A A KKV+RVILKGVGH FQIL KDQIS  R QE+MG I+AFI
Sbjct: 304 AGAGKKVDRVILKGVGHGFQILNKDQISTQRTQELMGSIRAFI 346

BLAST of Chy7G137540 vs. NCBI nr
Match: XP_022155020.1 (probable carboxylesterase 17 [Momordica charantia])

HSP 1 Score: 568 bits (1463), Expect = 1.52e-201
Identity = 283/345 (82.03%), Postives = 299/345 (86.67%), Query Frame = 0

Query: 21  KLKRMAAISIDPRLKPQIPKLRIQNEATAEEIEEVIRVYGDGRVERPPIIPNVSCGITAS 80
           K KRMAAISIDPRLK Q+PK  +Q  +TAEEIE +IRVY DG VERPPIIPNV C  TAS
Sbjct: 2   KFKRMAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTAS 61

Query: 81  DKRVIAKDVLIENFTNTWARIYVANTQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLAS 140
           +K V AKDVLI+NFTNTWARIY+AN+QR LPLLVYFHGGGFCVGSAAWSCYHEFLLDLAS
Sbjct: 62  NKGVSAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLAS 121

Query: 141 KAKCVILSVNYRLSPENRLPAAYEDGFNTLMWLKQQIITNPIEHKWWLGNSNVSKLFLVG 200
           KAKC ILS+NYRLSPENRLPAAYEDGFN LMWLKQQ I+ P EHKWWLGN N S LFL G
Sbjct: 122 KAKCAILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAG 181

Query: 201 DSAGANIAYNITTRLGLNEPTILKPLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALT 260
           DSAGANIAYNI TR GLNEP  LKPLTLKG+ILIQPFFGGESRTKSEKL AQPS+ SALT
Sbjct: 182 DSAGANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPST-SALT 241

Query: 261 LDAADTYWRLSLPAGANRDHTWSNPLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCA 320
           L  ADTYWRLSLP GA+RDH WSNPLVNG  KLR+TK+PP MVCISELDILVDRNLEFCA
Sbjct: 242 LATADTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCA 301

Query: 321 AMAAAKKKVERVILKGVGHSFQILKKDQISNVRRQEMMGCIQAFI 365
           AMAAA KKVERVI KGVGH FQIL KDQIS +R QEMM  IQ FI
Sbjct: 302 AMAAAGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFI 345

BLAST of Chy7G137540 vs. NCBI nr
Match: XP_022998605.1 (probable carboxylesterase 17 [Cucurbita maxima])

HSP 1 Score: 565 bits (1457), Expect = 9.29e-201
Identity = 280/341 (82.11%), Postives = 300/341 (87.98%), Query Frame = 0

Query: 25  MAAISIDPRLKPQIPKLRIQNEATAEEIEEVIRVYGDGRVERPPIIPNVSCGITASDKRV 84
           MAA+ IDPRLK Q+ K  +QN AT+EEIE +IRVY DG VERPPIIPNV+C  TAS+K V
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60

Query: 85  IAKDVLIENFTNTWARIYVANTQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 144
            AKDVLI+ FTNTWARIY+ N+QRNLPLLVYFHGGGFCVGSAAWSCYHEFLL+LASKAKC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 145 VILSVNYRLSPENRLPAAYEDGFNTLMWLKQQIITNPIEHKWWLGNSNVSKLFLVGDSAG 204
           VILS+NYRLSPENRLPAAYEDGFN +MWLKQQ ITNPIEHKWWLGN N SKLFL GDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 205 ANIAYNITTRLGLNEPTILKPLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALTLDAA 264
           ANIAYN  TRLG NE T+LKP TLKGIILIQPFFGGESRTKSEKL A PSS SALTL A+
Sbjct: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 265 DTYWRLSLPAGANRDHTWSNPLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCAAMAA 324
           DTYWRLSLP  ANRDH WSNPLVNG AKL+E K+PPLMVCISELDIL+DRNLEFCAAMAA
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300

Query: 325 AKKKVERVILKGVGHSFQILKKDQISNVRRQEMMGCIQAFI 365
           A KKVERVILKGVGH FQIL KDQIS  R QE+MG I+AFI
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFI 341

BLAST of Chy7G137540 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 320.5 bits (820), Expect = 1.8e-87
Identity = 171/321 (53.27%), Postives = 213/321 (66.36%), Query Frame = 0

Query: 50  EEIEEVIRVYGDGRVERPPIIPNVSCGITASDKRVIAKDVLIENFTNTWARIYV-----A 109
           EEIE +I+V+ DG VERPPI+P VS  I  S K   A D+ + N  +TW R+Y+     A
Sbjct: 30  EEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSK-ATAFDIKLSN--DTWTRVYIPDAAAA 89

Query: 110 NTQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYE 169
           +    LPLLVYFHGGGFCVGSAAWSCYH+FL  LA KA+CVI+SVNYRL+PE+RLPAAY+
Sbjct: 90  SPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPEHRLPAAYD 149

Query: 170 DGFNTLMWLKQQIITNPIEHKWWLGNSNVSKLFLVGDSAGANIAYNITTRLGLNEPTILK 229
           DG N + WL +Q I+    +  WL   N+S +FL GDSAGANIAY +  R+ +       
Sbjct: 150 DGVNVVSWLVKQQISTGGGYPSWLSKCNLSNVFLAGDSAGANIAYQVAVRI-MASGKYAN 209

Query: 230 PLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALTLDAADTYWRLSLPAGANRDHTWSN 289
            L LKGIILI PFFGGESRT SEK      S SALTL A+D YWRL+LP GA+RDH W N
Sbjct: 210 TLHLKGIILIHPFFGGESRTSSEKQQHHTKS-SALTLSASDAYWRLALPRGASRDHPWCN 269

Query: 290 PLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCAAMAAAKKKVERVILKGVGHSFQIL 349
           PL++        K+P  MV ++E DIL +RNLE C  M +  K+VE ++  GVGH+F IL
Sbjct: 270 PLMSSAG----AKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGGVGHAFHIL 329

Query: 350 KKDQISNVRRQEMMGCIQAFI 366
               +S  R  +MM  +  FI
Sbjct: 330 DNSSVSRDRIHDMMCRLHNFI 341

BLAST of Chy7G137540 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 8.7e-74
Identity = 142/321 (44.24%), Postives = 204/321 (63.55%), Query Frame = 0

Query: 50  EEIEEVIRVYGDGRVERPPIIPNVSCGITASDKRVIAKDVLIENFTNTWARIYVANTQ-- 109
           +E+E +I+VY DG VER  ++P V   +   +  V   DV+I+  TN WAR+YV  T   
Sbjct: 25  DEVEGLIKVYKDGHVERSQLLPCVDPSLPL-ELGVTCSDVVIDKLTNVWARLYVPMTTTK 84

Query: 110 ---RNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYE 169
                LPL+VYFHGGGFCVGSA+W CYHEFL  L+++++C+++SVNYRL+PEN LPAAYE
Sbjct: 85  SSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPLPAAYE 144

Query: 170 DGFNTLMWLKQQIITNPIEHKWWLGNSNVSKLFLVGDSAGANIAYNITTRLGLNEPTILK 229
           DG N ++WL +    N      W    +  ++FL GDSAG NIA  +  RL   E   LK
Sbjct: 145 DGVNAILWLNKARNDN-----LWAKQCDFGRIFLAGDSAGGNIAQQVAARLASPEDLALK 204

Query: 230 PLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALTLDAADTYWRLSLPAGANRDHTWSN 289
              ++G ILIQPF+ GE RT+SE+      + + LTL ++D +WR+SLP GANR+H +  
Sbjct: 205 ---IEGTILIQPFYSGEERTESERRVGNDKT-AVLTLASSDAWWRMSLPRGANREHPYCK 264

Query: 290 PLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCAAMAAAKKKVERVILKGVGHSFQIL 349
           P+      ++ + V   +VC++E+D+L+D N+E C      +  ++RV+ KGVGH+F IL
Sbjct: 265 PV---KMIIKSSTVTRTLVCVAEMDLLMDSNMEMC---DGNEDVIKRVLHKGVGHAFHIL 324

Query: 350 KKDQISNVRRQEMMGCIQAFI 366
            K Q+++    EM+  I AFI
Sbjct: 325 GKSQLAHTTTLEMLCQIDAFI 329

BLAST of Chy7G137540 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 187.6 bits (475), Expect = 1.8e-47
Identity = 107/300 (35.67%), Postives = 170/300 (56.67%), Query Frame = 0

Query: 49  AEEIEEVIRVYGDGRVERPPIIPNVSCGIT-ASDKRVIAKDVLIENFTNTWARIY---VA 108
           AE+   ++++  +G V R   I  ++  I   +++ V+ KD +     N   R+Y    A
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 109 NTQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYE 168
           + +  LP++V+FHGGGFC GS +W  +H F L LAS    +++S +YRL+PE+RLPAA+E
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 169 DGFNTLMWLKQQIITNPIEHKWWLGNSNV--SKLFLVGDSAGANIAYNITTRLGLNEPTI 228
           D    L WL  Q +++ + H W+   ++V   ++F+VGDS+G NIA+ +  R G +    
Sbjct: 131 DAEAVLTWLWDQAVSDGVNH-WFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIE 190

Query: 229 LKPLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALTLDAADTYWRLSLPAGANRDHTW 288
           L P+ ++G +L+ PFFGGE RT SE       S + L+LD  D +WRLSLP GA RDH  
Sbjct: 191 LTPVRVRGYVLMGPFFGGEERTNSE----NGPSEALLSLDLLDKFWRLSLPNGATRDHHM 250

Query: 289 SNPLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCAAM-AAAKKKVERVILKGVGHSF 342
           +NP       L    + P++V +   ++L DR  E+   +     K+V+ +  +   H F
Sbjct: 251 ANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of Chy7G137540 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 187.6 bits (475), Expect = 1.8e-47
Identity = 107/300 (35.67%), Postives = 170/300 (56.67%), Query Frame = 0

Query: 49  AEEIEEVIRVYGDGRVERPPIIPNVSCGIT-ASDKRVIAKDVLIENFTNTWARIY---VA 108
           AE+   ++++  +G V R   I  ++  I   +++ V+ KD +     N   R+Y    A
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 109 NTQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYE 168
           + +  LP++V+FHGGGFC GS +W  +H F L LAS    +++S +YRL+PE+RLPAA+E
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 169 DGFNTLMWLKQQIITNPIEHKWWLGNSNV--SKLFLVGDSAGANIAYNITTRLGLNEPTI 228
           D    L WL  Q +++ + H W+   ++V   ++F+VGDS+G NIA+ +  R G +    
Sbjct: 131 DAEAVLTWLWDQAVSDGVNH-WFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIE 190

Query: 229 LKPLTLKGIILIQPFFGGESRTKSEKLTAQPSSGSALTLDAADTYWRLSLPAGANRDHTW 288
           L P+ ++G +L+ PFFGGE RT SE       S + L+LD  D +WRLSLP GA RDH  
Sbjct: 191 LTPVRVRGYVLMGPFFGGEERTNSE----NGPSEALLSLDLLDKFWRLSLPNGATRDHHM 250

Query: 289 SNPLVNGVAKLRETKVPPLMVCISELDILVDRNLEFCAAM-AAAKKKVERVILKGVGHSF 342
           +NP       L    + P++V +   ++L DR  E+   +     K+V+ +  +   H F
Sbjct: 251 ANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of Chy7G137540 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 161.0 bits (406), Expect = 1.8e-39
Identity = 103/296 (34.80%), Postives = 157/296 (53.04%), Query Frame = 0

Query: 61  DGRVERPPIIPNVSCGITASD----KRVIAKDVLIENFTNTWARIYVANTQRN------- 120
           DG + R   + N  C     D       ++KD+ +    +TW R+Y+ ++  N       
Sbjct: 21  DGSITRD--LSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQ 80

Query: 121 -LPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCVILSVNYRLSPENRLPAAYEDGFN 180
            LP++VY+HGGGF + S     +H+F  ++A     +++S +YRL+PE+RLPAAY+DG  
Sbjct: 81  KLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVE 140

Query: 181 TLMWLKQQIITNPIEHKWWLGNSNVSKLFLVGDSAGANIAYNITTRLGLNEPTILKPLTL 240
            L W+K        + +W   +++ S +FL+G SAG N+AYN+  R  ++  + L PL +
Sbjct: 141 ALDWIKTS------DDEWIKSHADFSNVFLMGTSAGGNLAYNVGLR-SVDSVSDLSPLQI 200

Query: 241 KGIILIQPFFGGESRTKSEKLTAQPSSGSALTLDAADTYWRLSLPAGANRDHTWSNPLV- 300
           +G+IL  PFFGGE R++SE    +  +         D  W LSLP G +RDH +SNP V 
Sbjct: 201 RGLILHHPFFGGEERSESE---IRLMNDQVCPPIVTDVMWDLSLPVGVDRDHEYSNPTVG 260

Query: 301 NGVAKLRETKVPPL----MVCISELDILVDRNLEFCAAMAAAKKKVERVILKGVGH 340
           +G  KL   K+  L    M+   E D ++D  L+   A    KK VE V     GH
Sbjct: 261 DGSEKLE--KIGRLRWKVMMIGGEDDPMID--LQKDVAKLMKKKGVEVVEHYTGGH 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LFR72.5e-8653.27Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1[more]
Q9SX251.2e-7244.24Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1[more]
Q9FG132.6e-4635.67Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1[more]
Q0ZPV71.4e-3936.93Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1[more]
Q9LVB82.6e-3834.80Probable carboxylesterase 120 OS=Arabidopsis thaliana OX=3702 GN=CXE20 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G8X86.2e-16081.50probable carboxylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111452001 PE=4 ... [more]
A0A6J1DL904.0e-15982.03probable carboxylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111022166 PE=4... [more]
A0A6J1KCZ31.5e-15881.69probable carboxylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111493192 PE=4 SV... [more]
A0A2N9EQ591.6e-12064.24Abhydrolase_3 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS... [more]
A0A2R6RU976.2e-12063.19Carboxylesterase OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc04... [more]
Match NameE-valueIdentityDescription
XP_038904086.13.60e-22787.71probable carboxylesterase 17 [Benincasa hispida][more]
XP_022948283.11.66e-20281.92probable carboxylesterase 17 [Cucurbita moschata][more]
XP_023523365.11.36e-20181.34probable carboxylesterase 17 [Cucurbita pepo subsp. pepo][more]
XP_022155020.11.52e-20182.03probable carboxylesterase 17 [Momordica charantia][more]
XP_022998605.19.29e-20182.11probable carboxylesterase 17 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G16080.11.8e-8753.27carboxyesterase 17 [more]
AT1G68620.18.7e-7444.24alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.11.8e-4735.67alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.21.8e-4735.67alpha/beta-Hydrolases superfamily protein [more]
AT5G62180.11.8e-3934.80carboxyesterase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 63..367
e-value: 6.8E-70
score: 238.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 32..354
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 113..342
e-value: 6.9E-53
score: 179.7
NoneNo IPR availablePANTHERPTHR23024:SF409CARBOXYLESTERASE 6-RELATEDcoord: 37..366
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 37..366

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy7G137540.1Chy7G137540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity