Chy6G119500 (gene) Cucumber (hystrix) v1

Overview
NameChy6G119500
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionchloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic
LocationchrH06: 15001104 .. 15005039 (-)
RNA-Seq ExpressionChy6G119500
SyntenyChy6G119500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGGATTTCTACATTCTATCCAAAGAAACCAAGAAGCCCCTCCTTCCTCGTCAGGGAGCTTTGTGTGTGAGTGTGTTCAATTCCTCCTCATTTTCTTCTACGGCTTTAACAACATCCTCCACTTCTTCTGCTCGAAAATCCACTCTTCTTTTTACCAAACCATCCAATTTGCACGCAGGTTCAGTATTTTATTTTCTTCTTCTTCTTCTTCTTCTTCTTCGCTTTCGCTTTTTTCTCGTTTTTCCCCTTTAAAGTTCAACGAAACAACATGGTTCTTCAAGGGTTTTCTTTTTCCTGCTTTAGGTTTCAATGTTATATGCAGCATCCTTCCAAAGTGTCTCCTTATTTTCTAATTCGAGTAGTGCCCATTTCCATTTTCCTTCCACAAAGCCTCTGGTCTCCACCAGGCTCTCCGTACGAGCTTCATCGCCAGATAGTGATAATAATGGCCTTAAAGTGGAGTATACTCCTTGGCTTATTGTTGGACTGGGTAACCCAGGAAACAAGTATCATGGGACTCGGCACAATGTAATCAGACATTCTTTAACTCTGTGTAATGAACTTTTTGAATGAGTTTGGGATGGAATTTTCATAGTGTAGCAGTACTTTTAAACCAATCAAATTTCCTATCAAGTGCTTTCTATGAAGTGTTCCAAACTCACCCTCCCTTAACGTGCAGTGACACTAAATAACATTGCTCTTTTTGGCCTCTTGGGTTTGATTGTTCAGGTTGGTTTTGAAATGATTGATAGCATTGCTCAAGCGCAGGGAATTTTGATGAACACAATACAATCCAAAGCTCTAATTGGAATAGGTTGGCTTGTTTTCCCTTTCTATGTTATTGAATTGGGTTTCCATGTTACGTGGATTTGAAGTTCATTATGTTTCTTGAATAATCAGTACAGGTTCAATTGGAGAAGTACCAATTTTATTGGCAAAGCCTCAGACTTACATGAATTTCTGTGGAGAATCGGTTTGTCTTCTTGTCTCTCTTTTCTTCTGTTATTCAATAAGCATGGCACACAGCATACAGTTGAATGATGGTTTGTTTATTTCAGAAGTGTATGGATCCAAAGCTTAGTTTGAAGCAAAATATAAAAAGCTCACTGAGCTCCTTGTATGAGCTTATATTCATAGGGATAGATTGTTAGTTTTTTTCCATTGAAACAATTACATAGCATTAATGTCGCAGTTAGATAAGTATGTGGACAGGAGTTCTGTGGGTTTGGATATGTTTTACGCCTTTAGATGTTCTACTTAACCTTCAATTTTGTCTGAGTGTGATTGTTAGAGGCCCAAAGTCAACTGTGGTTTGCTCAAAGCGAACAAAATCTCTGATTGGATAGGTTTATTGTGATGATGGTCTGAAGATACTTAGATTTTTCATTTTTCTAACCTTACTGGTTACATATACTAGAAAACTCAGTTGATTCCTTATTAGATTTTGGATGAGGCTATAATATTGTAATTCCTACCATATCTGGCTGTATTTATTTGCACCACTTTATAGGTATGCACTCTATAGCGTTTTGTGGTAGAGCTTTGTTGTTTTGTAGCTAAGGATAGAAGCAAATTCTTTGAGGAAACCATTGTGATTGAACTATGACGTGGTTATTGTTGCCATCTACAAATAATTTACCTGATCTTATTAGATACATGGGGTTTCATCTCAAAATCAACTGGTAATGAGAGAAGTAACTCATCTATTTTAAAAATAAACAAATGTTGTGAGTGTCCCTTGATTTGTTCAATGCTAGATCCTCAAGATGTCCCCTCAAGATGAATCTTTGGATTCACCATTCTAGAATCAAATTCAATGTTTTGTTTCCGGATTGAATATTGAATGCTCGTTTGGCTTAATAAGCTAAAATAGCCGATTAGATACATGAAATTTCATCTCAAAACCAATTGGCAATGTTATGAGTAGTTGATCTCAATATCTTAAGAACCCTGCATATGGATGTTTTGTTCTACAGCTGCTAAGACTATGAATCTTTCCTTCATCAGTTGAATTATTTTTCCATAATGACGAGATAGTTATTCCCCTCACATCACTTTCTGCTTAAATTTTATAGGTTGGACCTCTTGCTGCTCATTACCAAATACCTTTACGGCACGTCTTGTTGGTATAATTTACAATTTCTCTTAGTAATCCTTATTTGTTACTGTACAAAATGACTAGATGAATGGAATTCTGTACATGCTTACCATTGTAGACTTATGATGAAATGAGCTTGCCAAATGGCGTTCTTAGGCTGCAGCCAAAAGGAGGACATGGCCATCATAATGGGTAGGTTTATGAAATATTATTTAATATCGGTTTCACTCTTATTTTTGTTCTAGTTTTAATTGGTACCTCATACTTTCAATTTTTCTAACCTTGTTATGTTGTTATGTACCTATGGTAATGTTTGGAAGTCCATTTGATATCACCACAACTGCTTCTCACTTAAAATCATCATGCTTGAGAGTAACATAAAAGTACATTAGGAAAATGTCAAAAGTATATAAAGTGCTTTACGAGAAAGTTTCTACAGAGTGCTTTTGGCCCAATTGCTGGACCAGCAAGCACACTTGACAACAGATTAAAAAATGTAGAACTAAAAATAAAATTAGTTTGTAATTTAGCACCTTCTACTGAAGGCCTTATAATTAAAATATCTTTTTAAAAGCACCCTCAAACATCTTTTAGTATAAGCACCTTGCTGTATTGTATATTTTATTGGGGCATGTGTTGGAACTTCTTTGACAAGCTTATTAAGTCATTAAGAAAATTGAAGCTCAATGGACTATTTCTTGTCGAAACTTCCCACTGAAGACTGTTAGCCCCCCTATTATTCACACTTACAAACGAAGGGGCACAAAAGGTAATAACCAGGTGACAGCTTAACCTATGAGGATTATGGAAGGAATATGTTGCTGTGGGACCCATGGGAAGGCATGGGAGTATATCCGAATGTTTTTGGGATTGCTGAAGGTAGGTCATATTTTGAGAAAAGTTGCAGAAACTTTGGAGGAGAAGGCCAGCATTCTCGAATTAGCTGGAGAGTCTTTCCTTTTATATTTTGATATTTCTTGTGTTATTTTCTGCTTTGTTTTTTTCCACTGTTTAGAGGATATCTACATGAACCTTGTTTCCTACTCTATTTCTATCAATATATAAATACAGAGTACTGTCTCTGTTCCTCTATTGTGTTGTTCTATTGTGTGTTGGGACGTTTCTGAGTGGCAGGTTTTTGGGATCCTAACAAAGACTTGATGAACGTGTTTCATATTAGCATTTTATAGTTGATTTGATTGGACATGCATTTTCCTTCGCTTATTAGCCATTCAACTTGGAAGTCATGTTCTTCTCTTATTTGTCATTTCATAGTTGAGAAATTTATGGCAGCGTGTGCATAAAAAGTTGTTATTGATTACATTAGTTATACAGGGTGAAGAGTGTAATGGGACACCTGGATGGTCGACGTGAGTTTCCTCGGCTATGCATCGGTATGCATCTCAGTTAACATTAGTTTGAAAATGTTCATTAAGTTTCTACTTCAGGAAGTGTAAACAACGACATTGCTCTTGTTATTACTATATCTAGGAATTGGAAATCCACCTGGGACTATGGACATGAAAGCTTTTCTCCTGCAGAAGTTCAGTTCAGTTGAGCGAAAACAGGTGAAACTTCCTTTGCGTTCTAATACTGTTCTGCTCTGCATTACTTCATACGTCGCAACCTGATGACTTCGAAGCTGTACTTGCCTGGTGGTAGAACACTATTGCTTAGTGCATTTTTCTTTTTTGACTTTTGTTTGTGCAACCCAACAGATCGATACAGCTCTTGAACAAGGGGTTGAGGCAGTGAGAACCCTTGCACTGAATGGATTTAGCCAAAGAGTAAACAGATTCAATTTGTCCCAGAAATACAAGTATCATAAGGTTTGA

mRNA sequence

ATGTGGGATTTCTACATTCTATCCAAAGAAACCAAGAAGCCCCTCCTTCCTCGTCAGGGAGCTTTGTGTGTGAGTGTGTTCAATTCCTCCTCATTTTCTTCTACGGCTTTAACAACATCCTCCACTTCTTCTGCTCGAAAATCCACTCTTCTTTTTACCAAACCATCCAATTTGCACGCAGCATCCTTCCAAAGTGTCTCCTTATTTTCTAATTCGAGTAGTGCCCATTTCCATTTTCCTTCCACAAAGCCTCTGGTCTCCACCAGGCTCTCCGTACGAGCTTCATCGCCAGATAGTGATAATAATGGCCTTAAAGTGGAGTATACTCCTTGGCTTATTGTTGGACTGGGTAACCCAGGAAACAAGTATCATGGGACTCGGCACAATGTTGGTTTTGAAATGATTGATAGCATTGCTCAAGCGCAGGGAATTTTGATGAACACAATACAATCCAAAGCTCTAATTGGAATAGGTTCAATTGGAGAAGTACCAATTTTATTGGCAAAGCCTCAGACTTACATGAATTTCTGTGGAGAATCGGTTGGACCTCTTGCTGCTCATTACCAAATACCTTTACGGCACGTCTTGTTGACTTATGATGAAATGAGCTTGCCAAATGGCGTTCTTAGGCTGCAGCCAAAAGGAGGACATGGCCATCATAATGGGGTGAAGAGTGTAATGGGACACCTGGATGGTCGACGTGAGTTTCCTCGGCTATGCATCGGAATTGGAAATCCACCTGGGACTATGGACATGAAAGCTTTTCTCCTGCAGAAGTTCAGTTCAGTTGAGCGAAAACAGATCGATACAGCTCTTGAACAAGGGGTTGAGGCAGTGAGAACCCTTGCACTGAATGGATTTAGCCAAAGAGTAAACAGATTCAATTTGTCCCAGAAATACAAGTATCATAAGGTTTGA

Coding sequence (CDS)

ATGTGGGATTTCTACATTCTATCCAAAGAAACCAAGAAGCCCCTCCTTCCTCGTCAGGGAGCTTTGTGTGTGAGTGTGTTCAATTCCTCCTCATTTTCTTCTACGGCTTTAACAACATCCTCCACTTCTTCTGCTCGAAAATCCACTCTTCTTTTTACCAAACCATCCAATTTGCACGCAGCATCCTTCCAAAGTGTCTCCTTATTTTCTAATTCGAGTAGTGCCCATTTCCATTTTCCTTCCACAAAGCCTCTGGTCTCCACCAGGCTCTCCGTACGAGCTTCATCGCCAGATAGTGATAATAATGGCCTTAAAGTGGAGTATACTCCTTGGCTTATTGTTGGACTGGGTAACCCAGGAAACAAGTATCATGGGACTCGGCACAATGTTGGTTTTGAAATGATTGATAGCATTGCTCAAGCGCAGGGAATTTTGATGAACACAATACAATCCAAAGCTCTAATTGGAATAGGTTCAATTGGAGAAGTACCAATTTTATTGGCAAAGCCTCAGACTTACATGAATTTCTGTGGAGAATCGGTTGGACCTCTTGCTGCTCATTACCAAATACCTTTACGGCACGTCTTGTTGACTTATGATGAAATGAGCTTGCCAAATGGCGTTCTTAGGCTGCAGCCAAAAGGAGGACATGGCCATCATAATGGGGTGAAGAGTGTAATGGGACACCTGGATGGTCGACGTGAGTTTCCTCGGCTATGCATCGGAATTGGAAATCCACCTGGGACTATGGACATGAAAGCTTTTCTCCTGCAGAAGTTCAGTTCAGTTGAGCGAAAACAGATCGATACAGCTCTTGAACAAGGGGTTGAGGCAGTGAGAACCCTTGCACTGAATGGATTTAGCCAAAGAGTAAACAGATTCAATTTGTCCCAGAAATACAAGTATCATAAGGTTTGA

Protein sequence

MWDFYILSKETKKPLLPRQGALCVSVFNSSSFSSTALTTSSTSSARKSTLLFTKPSNLHAASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSDNNGLKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHVLLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNLSQKYKYHKV*
Homology
BLAST of Chy6G119500 vs. ExPASy Swiss-Prot
Match: Q9LF14 (Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CRS2B PE=2 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 1.4e-98
Identity = 170/230 (73.91%), Postives = 199/230 (86.52%), Query Frame = 0

Query: 77  FHFPSTKPLV-STRLSVRASSPDSDNNGLKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMI 136
           +HF + KP+    R  V +S+   +++  KVEYTPWLIVGLGNPGNKYHGTRHNVGFEMI
Sbjct: 11  YHFQTQKPVFRKQRFRVCSSTSGGEDDRFKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMI 70

Query: 137 DSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHV 196
           D +A+ +G+LMNTIQSKALIGIG+I +VPILLAKPQTYMNF GESVG LA+HY++PLRH+
Sbjct: 71  DVLARKEGVLMNTIQSKALIGIGAIEDVPILLAKPQTYMNFSGESVGSLASHYRVPLRHI 130

Query: 197 LLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAF 256
           L+ YDEM+LPNGVLRLQPKGG G+HNGVKSVMGHLDGRR FPRL IGIG PPG MDMKAF
Sbjct: 131 LMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAF 190

Query: 257 LLQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNLSQKYKYHKV 306
           LLQKFS +E+KQI+ ALEQG EAV+TL LNGF+Q ++RFNL QKYK+HKV
Sbjct: 191 LLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQGISRFNLVQKYKFHKV 240

BLAST of Chy6G119500 vs. ExPASy Swiss-Prot
Match: Q9FKN4 (Chloroplastic group IIB intron splicing facilitator CRS2-A, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CRS2A PE=2 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 3.4e-92
Identity = 164/229 (71.62%), Postives = 185/229 (80.79%), Query Frame = 0

Query: 77  FHFPSTKPLVSTRLSVRASSPDSDNNGLKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMID 136
           + F  TK       S+RAS P SDN  LK EYTPWLIVGLGNPG KY+GTRHN+GFEMID
Sbjct: 18  YKFSQTKSNSKRFSSLRASLPVSDNKLLKFEYTPWLIVGLGNPGLKYYGTRHNIGFEMID 77

Query: 137 SIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHVL 196
            IA+A  I MNTIQSKAL+GIGS+GEVPILL KPQ YMNF GESVGPLAA+YQIPLRH+L
Sbjct: 78  HIARATDISMNTIQSKALVGIGSVGEVPILLVKPQGYMNFSGESVGPLAAYYQIPLRHIL 137

Query: 197 LTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFL 256
           + YD+M L NGVLRLQPKGGH  HNG+K+V  HL+G R +PRL IGIGNPPG MDMKAFL
Sbjct: 138 MIYDDMGLSNGVLRLQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFL 197

Query: 257 LQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNLSQKYKYHKV 306
           LQKFS +ERKQ+D  LEQGVE V+TL   GFS  ++RFNL QKYK+HKV
Sbjct: 198 LQKFSPLERKQMDEGLEQGVEGVKTLVEEGFSDSISRFNLGQKYKFHKV 246

BLAST of Chy6G119500 vs. ExPASy Swiss-Prot
Match: Q9M5P4 (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Zea mays OX=4577 GN=CRS2 PE=1 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 2.7e-89
Identity = 165/249 (66.27%), Postives = 193/249 (77.51%), Query Frame = 0

Query: 72  SSSAHFHFP-----------STKPLVSTR----LSVRASSPDSDNNGLKVEYTPWLIVGL 131
           S+S+HF  P            T PL   R     +V +S PD       VEYTPWLI GL
Sbjct: 10  STSSHFSAPFLPSFRMPRKSLTAPLHRIRRPRPFTVVSSVPDPAAG--PVEYTPWLIAGL 69

Query: 132 GNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNF 191
           GNPGNKY+GTRHNVGFEM+D IA  +GI MNTIQSK+L+GIGSIGEVP+L+ KPQ+YMN+
Sbjct: 70  GNPGNKYYGTRHNVGFEMVDRIAAEEGITMNTIQSKSLLGIGSIGEVPVLVVKPQSYMNY 129

Query: 192 CGESVGPLAAHYQIPLRHVLLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREF 251
            GE++GPLAA+YQ+PLRH+LL YD+ SLPNGVLRLQ KGGHG HNG+++V+ HLDGRREF
Sbjct: 130 SGEAIGPLAAYYQVPLRHILLIYDDTSLPNGVLRLQKKGGHGRHNGLQNVIEHLDGRREF 189

Query: 252 PRLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNL 306
           PRL IGIG+PPG MD +AFLLQKFSS ER QIDTALEQGV+AVRTL L GFS    RFNL
Sbjct: 190 PRLSIGIGSPPGKMDPRAFLLQKFSSEERVQIDTALEQGVDAVRTLVLKGFSGSTERFNL 249

BLAST of Chy6G119500 vs. ExPASy Swiss-Prot
Match: Q5ZCL8 (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0132800 PE=2 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.2e-86
Identity = 169/284 (59.51%), Postives = 204/284 (71.83%), Query Frame = 0

Query: 22  LCVSVFNSSSFSSTALTTSSTSSARKSTLLFTKPSNLHAASFQSVSLFSNSSSAHFHFPS 81
           + ++V   +S   + LTTSS    R+  LL      L AA+                 P 
Sbjct: 1   MSLAVATPASARLSPLTTSSPEPCRRRRLL------LSAAA-----------------PL 60

Query: 82  TKPLVSTRLSVRASSPDSDNNGLKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQA 141
            +  +  R++V AS PD        EYTPWLI GLGNPG+KYHGTRHNVGFEM+D IA+ 
Sbjct: 61  RRTRLRRRIAVVASVPDP--AARPAEYTPWLIAGLGNPGSKYHGTRHNVGFEMVDRIARD 120

Query: 142 QGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHVLLTYDE 201
           +GI MNTIQSK+L+GIGSIGEVP+LL KPQ+Y+N+ GE++GPLAA+YQ+PLRH+L+ YDE
Sbjct: 121 EGITMNTIQSKSLLGIGSIGEVPVLLVKPQSYINYSGEAIGPLAAYYQVPLRHILVMYDE 180

Query: 202 MSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFS 261
           MSLPNGVLRLQ KGGHG HNG+++VM  LD  RE PRL IGIG+PPG MD +AFLLQKFS
Sbjct: 181 MSLPNGVLRLQRKGGHGRHNGLQNVMECLDSSRELPRLSIGIGSPPGKMDTRAFLLQKFS 240

Query: 262 SVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNLSQKYKYHKV 306
           S ER QIDTALEQGV+AVRTL L GFS  + RFNL QKYK+H V
Sbjct: 241 SEERLQIDTALEQGVDAVRTLVLKGFSGSIERFNLVQKYKFHSV 259

BLAST of Chy6G119500 vs. ExPASy Swiss-Prot
Match: Q6NLS8 (Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g19830 PE=2 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.2e-65
Identity = 116/197 (58.88%), Postives = 148/197 (75.13%), Query Frame = 0

Query: 108 YTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILL 167
           + PWL +GLGNPG+KY GTRHN+GFEMID  A++ GI MN +  KA++G G + ++P++L
Sbjct: 16  HRPWLFLGLGNPGDKYKGTRHNIGFEMIDVFAESVGIQMNLVNFKAIMGQGFVADLPVIL 75

Query: 168 AKPQTYMNFCGESVGPLAAHYQIPLRHVLLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVM 227
           AKPQTYMN  GES GPLAA+Y++PL  VL+ +D+M LP GVLRLQ KGGHG HNG+KSVM
Sbjct: 76  AKPQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPCGVLRLQEKGGHGCHNGLKSVM 135

Query: 228 GHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDTALEQGVEAVRTLALNGF 287
            H  G REF RL IGIG PPG MD KAFLLQKFS   R+++D AL +GV+A++ +    F
Sbjct: 136 NHFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPARERMDKALAEGVDALKLVLAKDF 195

Query: 288 SQRVNRFNLSQKYKYHK 305
            +    FN+ QKYK+ K
Sbjct: 196 GESWRLFNVEQKYKHLK 212

BLAST of Chy6G119500 vs. ExPASy TrEMBL
Match: A0A0A0LCC6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G257600 PE=3 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 2.3e-136
Identity = 243/248 (97.98%), Postives = 245/248 (98.79%), Query Frame = 0

Query: 58  LHAASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSDNNGLKVEYTPWLIVGLG 117
           L AASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSD+NGLKVEYTPWLIVGLG
Sbjct: 2   LFAASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSDSNGLKVEYTPWLIVGLG 61

Query: 118 NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 177
           NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC
Sbjct: 62  NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 121

Query: 178 GESVGPLAAHYQIPLRHVLLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 237
           GESVGPLAAHYQIPLRHVLLTYDEM+LPNGVLRLQPK GHGHHNGVKSVMGHLDGRREFP
Sbjct: 122 GESVGPLAAHYQIPLRHVLLTYDEMNLPNGVLRLQPKAGHGHHNGVKSVMGHLDGRREFP 181

Query: 238 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNLS 297
           RLCIGIGNPPGTMDMKAFLLQKFSSVERKQID ALEQGVEAVRTLALNGFSQRVNRFNLS
Sbjct: 182 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLS 241

Query: 298 QKYKYHKV 306
           QKYKYHKV
Sbjct: 242 QKYKYHKV 249

BLAST of Chy6G119500 vs. ExPASy TrEMBL
Match: A0A1S3CFG5 (chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103500129 PE=3 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 3.7e-134
Identity = 240/249 (96.39%), Postives = 245/249 (98.39%), Query Frame = 0

Query: 58  LHAASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSD-NNGLKVEYTPWLIVGL 117
           L+AASFQSVSLFSNSS+AH+H PSTKPLVSTRLSVRAS PDSD NNG+KVEYTPWLIVGL
Sbjct: 2   LYAASFQSVSLFSNSSTAHYHSPSTKPLVSTRLSVRASLPDSDNNNGVKVEYTPWLIVGL 61

Query: 118 GNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNF 177
           GNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNF
Sbjct: 62  GNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNF 121

Query: 178 CGESVGPLAAHYQIPLRHVLLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREF 237
           CGESVGPLAAHYQIPLRH+LLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREF
Sbjct: 122 CGESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREF 181

Query: 238 PRLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNL 297
           PRLCIGIGNPPGTMDMKAFLLQKFSSVERKQID ALEQGVEAVRTLALNGFSQRVNRFNL
Sbjct: 182 PRLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNL 241

Query: 298 SQKYKYHKV 306
           SQKYKYHKV
Sbjct: 242 SQKYKYHKV 250

BLAST of Chy6G119500 vs. ExPASy TrEMBL
Match: A0A6J1D2Z6 (chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111016856 PE=3 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 7.2e-122
Identity = 224/248 (90.32%), Postives = 230/248 (92.74%), Query Frame = 0

Query: 58  LHAASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSDNNGLKVEYTPWLIVGLG 117
           L+A S QSV L SNSSSAHF     K LV TRLSVRAS  D+D NG+KVEYTPWLIVGLG
Sbjct: 2   LYAVSVQSVCLLSNSSSAHFRPRLRKRLVPTRLSVRASLLDND-NGVKVEYTPWLIVGLG 61

Query: 118 NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 177
           NPGNKYHGTRHNVGFEMIDSIAQAQ ILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC
Sbjct: 62  NPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 121

Query: 178 GESVGPLAAHYQIPLRHVLLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 237
           GESVGPLAAHYQIPLRH+LL YDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP
Sbjct: 122 GESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 181

Query: 238 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNLS 297
           RLCIGIGNPPGTMDMKAFLLQKF SVERKQID ALEQGVEAVRTLALNGFSQRVNRFNL+
Sbjct: 182 RLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT 241

Query: 298 QKYKYHKV 306
           QKYKY+KV
Sbjct: 242 QKYKYNKV 248

BLAST of Chy6G119500 vs. ExPASy TrEMBL
Match: A0A6J1K6X4 (chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111492843 PE=3 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 1.2e-121
Identity = 223/248 (89.92%), Postives = 230/248 (92.74%), Query Frame = 0

Query: 58  LHAASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSDNNGLKVEYTPWLIVGLG 117
           L+AAS  SV LFSNSSSAHF     K LV TRLSVRAS  D+D NG+KVEYTPWLIVGLG
Sbjct: 2   LYAASVPSVCLFSNSSSAHFPSRFPKRLVPTRLSVRASLQDND-NGVKVEYTPWLIVGLG 61

Query: 118 NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 177
           NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC
Sbjct: 62  NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 121

Query: 178 GESVGPLAAHYQIPLRHVLLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 237
           GESVGPLA HYQIPLRH+LL YDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP
Sbjct: 122 GESVGPLAVHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 181

Query: 238 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNLS 297
           RLCIGIGNPPGTMDMKAFLLQKFSSVERKQID ALEQGVEAVRTLAL GFS+ V+RFNL+
Sbjct: 182 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDAALEQGVEAVRTLALKGFSKGVDRFNLT 241

Query: 298 QKYKYHKV 306
           QKYKYHKV
Sbjct: 242 QKYKYHKV 248

BLAST of Chy6G119500 vs. ExPASy TrEMBL
Match: A0A6J1G9M7 (chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111452203 PE=3 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 4.7e-121
Identity = 221/248 (89.11%), Postives = 230/248 (92.74%), Query Frame = 0

Query: 58  LHAASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSDNNGLKVEYTPWLIVGLG 117
           L+AAS  SV LFSNSS AHF     K LV TRLSVRAS PD+D NG+KVEYTPWLIVGLG
Sbjct: 2   LYAASVPSVCLFSNSSIAHFPSRFPKRLVPTRLSVRASLPDND-NGVKVEYTPWLIVGLG 61

Query: 118 NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 177
           NPGNKYHGTRHNVGF+MIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC
Sbjct: 62  NPGNKYHGTRHNVGFDMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 121

Query: 178 GESVGPLAAHYQIPLRHVLLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 237
           GESVGPLA HYQIPLRH+LL YDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP
Sbjct: 122 GESVGPLAVHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 181

Query: 238 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNLS 297
           RLCIGIGNPPGTMD+KAFLLQKFSSVERKQID ALEQGVEAVRTLAL GFS+ V+RFNL+
Sbjct: 182 RLCIGIGNPPGTMDIKAFLLQKFSSVERKQIDAALEQGVEAVRTLALKGFSKGVDRFNLT 241

Query: 298 QKYKYHKV 306
           QKYKYHKV
Sbjct: 242 QKYKYHKV 248

BLAST of Chy6G119500 vs. NCBI nr
Match: XP_004145630.1 (chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic [Cucumis sativus] >KGN57706.1 hypothetical protein Csa_010483 [Cucumis sativus])

HSP 1 Score: 492 bits (1267), Expect = 1.99e-174
Identity = 243/248 (97.98%), Postives = 245/248 (98.79%), Query Frame = 0

Query: 58  LHAASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSDNNGLKVEYTPWLIVGLG 117
           L AASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSD+NGLKVEYTPWLIVGLG
Sbjct: 2   LFAASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSDSNGLKVEYTPWLIVGLG 61

Query: 118 NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 177
           NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC
Sbjct: 62  NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 121

Query: 178 GESVGPLAAHYQIPLRHVLLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 237
           GESVGPLAAHYQIPLRHVLLTYDEM+LPNGVLRLQPK GHGHHNGVKSVMGHLDGRREFP
Sbjct: 122 GESVGPLAAHYQIPLRHVLLTYDEMNLPNGVLRLQPKAGHGHHNGVKSVMGHLDGRREFP 181

Query: 238 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNLS 297
           RLCIGIGNPPGTMDMKAFLLQKFSSVERKQID ALEQGVEAVRTLALNGFSQRVNRFNLS
Sbjct: 182 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLS 241

Query: 298 QKYKYHKV 305
           QKYKYHKV
Sbjct: 242 QKYKYHKV 249

BLAST of Chy6G119500 vs. NCBI nr
Match: XP_008461557.1 (PREDICTED: chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic [Cucumis melo])

HSP 1 Score: 485 bits (1248), Expect = 1.62e-171
Identity = 240/249 (96.39%), Postives = 245/249 (98.39%), Query Frame = 0

Query: 58  LHAASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSDNN-GLKVEYTPWLIVGL 117
           L+AASFQSVSLFSNSS+AH+H PSTKPLVSTRLSVRAS PDSDNN G+KVEYTPWLIVGL
Sbjct: 2   LYAASFQSVSLFSNSSTAHYHSPSTKPLVSTRLSVRASLPDSDNNNGVKVEYTPWLIVGL 61

Query: 118 GNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNF 177
           GNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNF
Sbjct: 62  GNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNF 121

Query: 178 CGESVGPLAAHYQIPLRHVLLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREF 237
           CGESVGPLAAHYQIPLRH+LLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREF
Sbjct: 122 CGESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREF 181

Query: 238 PRLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNL 297
           PRLCIGIGNPPGTMDMKAFLLQKFSSVERKQID ALEQGVEAVRTLALNGFSQRVNRFNL
Sbjct: 182 PRLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNL 241

Query: 298 SQKYKYHKV 305
           SQKYKYHKV
Sbjct: 242 SQKYKYHKV 250

BLAST of Chy6G119500 vs. NCBI nr
Match: XP_038899626.1 (chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic [Benincasa hispida])

HSP 1 Score: 472 bits (1215), Expect = 1.61e-166
Identity = 234/248 (94.35%), Postives = 241/248 (97.18%), Query Frame = 0

Query: 58  LHAASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSDNNGLKVEYTPWLIVGLG 117
           L+AAS QSV LFSNSS+AHFH  STKPLVSTRLS+RAS PD+DN G+KVEYTPWLIVGLG
Sbjct: 2   LYAASVQSVCLFSNSSTAHFHPRSTKPLVSTRLSIRASLPDNDN-GVKVEYTPWLIVGLG 61

Query: 118 NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 177
           NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC
Sbjct: 62  NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 121

Query: 178 GESVGPLAAHYQIPLRHVLLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 237
           GESVGPLAAHYQIPLRH+LLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP
Sbjct: 122 GESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 181

Query: 238 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNLS 297
           RLCIGIGNPPGTMDMKAFLLQKFSSVERKQID ALEQGVEAVRTLALNGFSQRVNRFNL+
Sbjct: 182 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT 241

Query: 298 QKYKYHKV 305
           QKYKYHKV
Sbjct: 242 QKYKYHKV 248

BLAST of Chy6G119500 vs. NCBI nr
Match: KAG6607055.1 (Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 446 bits (1147), Expect = 1.93e-155
Identity = 233/282 (82.62%), Postives = 245/282 (86.88%), Query Frame = 0

Query: 31  SFSSTALTTSSTSSARKSTLLF-------TKPSNLHAASFQSVSLFSNSSSAHFHFPSTK 90
           S SST  T S+ +S   + LLF        + S L+AAS  SV LFSNSS AHF     K
Sbjct: 14  SSSSTPFTASTAASVHPNQLLFQPNHPIYAQVSMLYAASVPSVCLFSNSSIAHFPSRFPK 73

Query: 91  PLVSTRLSVRASSPDSDNNGLKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQG 150
            LV TRLSVRAS PD+DN G+KVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQG
Sbjct: 74  RLVPTRLSVRASLPDNDN-GVKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQG 133

Query: 151 ILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHVLLTYDEMS 210
           ILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLA HYQIPLRH+LL YDEMS
Sbjct: 134 ILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAVHYQIPLRHILLVYDEMS 193

Query: 211 LPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFSSV 270
           LPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMD+KAFLLQKFSSV
Sbjct: 194 LPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDIKAFLLQKFSSV 253

Query: 271 ERKQIDTALEQGVEAVRTLALNGFSQRVNRFNLSQKYKYHKV 305
           ERKQID ALEQGVEAVRTLAL GFS+ V+RFNL+QKYKYHKV
Sbjct: 254 ERKQIDAALEQGVEAVRTLALKGFSKGVDRFNLTQKYKYHKV 294

BLAST of Chy6G119500 vs. NCBI nr
Match: XP_022148094.1 (chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic-like [Momordica charantia])

HSP 1 Score: 444 bits (1142), Expect = 2.12e-155
Identity = 224/248 (90.32%), Postives = 230/248 (92.74%), Query Frame = 0

Query: 58  LHAASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSDNNGLKVEYTPWLIVGLG 117
           L+A S QSV L SNSSSAHF     K LV TRLSVRAS  D+DN G+KVEYTPWLIVGLG
Sbjct: 2   LYAVSVQSVCLLSNSSSAHFRPRLRKRLVPTRLSVRASLLDNDN-GVKVEYTPWLIVGLG 61

Query: 118 NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 177
           NPGNKYHGTRHNVGFEMIDSIAQAQ ILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC
Sbjct: 62  NPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 121

Query: 178 GESVGPLAAHYQIPLRHVLLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 237
           GESVGPLAAHYQIPLRH+LL YDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP
Sbjct: 122 GESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 181

Query: 238 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNLS 297
           RLCIGIGNPPGTMDMKAFLLQKF SVERKQID ALEQGVEAVRTLALNGFSQRVNRFNL+
Sbjct: 182 RLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT 241

Query: 298 QKYKYHKV 305
           QKYKY+KV
Sbjct: 242 QKYKYNKV 248

BLAST of Chy6G119500 vs. TAIR 10
Match: AT5G16140.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 360.9 bits (925), Expect = 1.0e-99
Identity = 170/230 (73.91%), Postives = 199/230 (86.52%), Query Frame = 0

Query: 77  FHFPSTKPLV-STRLSVRASSPDSDNNGLKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMI 136
           +HF + KP+    R  V +S+   +++  KVEYTPWLIVGLGNPGNKYHGTRHNVGFEMI
Sbjct: 11  YHFQTQKPVFRKQRFRVCSSTSGGEDDRFKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMI 70

Query: 137 DSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHV 196
           D +A+ +G+LMNTIQSKALIGIG+I +VPILLAKPQTYMNF GESVG LA+HY++PLRH+
Sbjct: 71  DVLARKEGVLMNTIQSKALIGIGAIEDVPILLAKPQTYMNFSGESVGSLASHYRVPLRHI 130

Query: 197 LLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAF 256
           L+ YDEM+LPNGVLRLQPKGG G+HNGVKSVMGHLDGRR FPRL IGIG PPG MDMKAF
Sbjct: 131 LMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAF 190

Query: 257 LLQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNLSQKYKYHKV 306
           LLQKFS +E+KQI+ ALEQG EAV+TL LNGF+Q ++RFNL QKYK+HKV
Sbjct: 191 LLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQGISRFNLVQKYKFHKV 240

BLAST of Chy6G119500 vs. TAIR 10
Match: AT5G16140.2 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 360.9 bits (925), Expect = 1.0e-99
Identity = 170/230 (73.91%), Postives = 199/230 (86.52%), Query Frame = 0

Query: 77  FHFPSTKPLV-STRLSVRASSPDSDNNGLKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMI 136
           +HF + KP+    R  V +S+   +++  KVEYTPWLIVGLGNPGNKYHGTRHNVGFEMI
Sbjct: 11  YHFQTQKPVFRKQRFRVCSSTSGGEDDRFKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMI 70

Query: 137 DSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHV 196
           D +A+ +G+LMNTIQSKALIGIG+I +VPILLAKPQTYMNF GESVG LA+HY++PLRH+
Sbjct: 71  DVLARKEGVLMNTIQSKALIGIGAIEDVPILLAKPQTYMNFSGESVGSLASHYRVPLRHI 130

Query: 197 LLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAF 256
           L+ YDEM+LPNGVLRLQPKGG G+HNGVKSVMGHLDGRR FPRL IGIG PPG MDMKAF
Sbjct: 131 LMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAF 190

Query: 257 LLQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNLSQKYKYHKV 306
           LLQKFS +E+KQI+ ALEQG EAV+TL LNGF+Q ++RFNL QKYK+HKV
Sbjct: 191 LLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQGISRFNLVQKYKFHKV 240

BLAST of Chy6G119500 vs. TAIR 10
Match: AT5G38290.2 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 339.7 bits (870), Expect = 2.4e-93
Identity = 164/229 (71.62%), Postives = 185/229 (80.79%), Query Frame = 0

Query: 77  FHFPSTKPLVSTRLSVRASSPDSDNNGLKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMID 136
           + F  TK       S+RAS P SDN  LK EYTPWLIVGLGNPG KY+GTRHN+GFEMID
Sbjct: 22  YKFSQTKSNSKRFSSLRASLPVSDNKLLKFEYTPWLIVGLGNPGLKYYGTRHNIGFEMID 81

Query: 137 SIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHVL 196
            IA+A  I MNTIQSKAL+GIGS+GEVPILL KPQ YMNF GESVGPLAA+YQIPLRH+L
Sbjct: 82  HIARATDISMNTIQSKALVGIGSVGEVPILLVKPQGYMNFSGESVGPLAAYYQIPLRHIL 141

Query: 197 LTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFL 256
           + YD+M L NGVLRLQPKGGH  HNG+K+V  HL+G R +PRL IGIGNPPG MDMKAFL
Sbjct: 142 MIYDDMGLSNGVLRLQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFL 201

Query: 257 LQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNLSQKYKYHKV 306
           LQKFS +ERKQ+D  LEQGVE V+TL   GFS  ++RFNL QKYK+HKV
Sbjct: 202 LQKFSPLERKQMDEGLEQGVEGVKTLVEEGFSDSISRFNLGQKYKFHKV 250

BLAST of Chy6G119500 vs. TAIR 10
Match: AT5G38290.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 339.7 bits (870), Expect = 2.4e-93
Identity = 164/229 (71.62%), Postives = 185/229 (80.79%), Query Frame = 0

Query: 77  FHFPSTKPLVSTRLSVRASSPDSDNNGLKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMID 136
           + F  TK       S+RAS P SDN  LK EYTPWLIVGLGNPG KY+GTRHN+GFEMID
Sbjct: 18  YKFSQTKSNSKRFSSLRASLPVSDNKLLKFEYTPWLIVGLGNPGLKYYGTRHNIGFEMID 77

Query: 137 SIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHVL 196
            IA+A  I MNTIQSKAL+GIGS+GEVPILL KPQ YMNF GESVGPLAA+YQIPLRH+L
Sbjct: 78  HIARATDISMNTIQSKALVGIGSVGEVPILLVKPQGYMNFSGESVGPLAAYYQIPLRHIL 137

Query: 197 LTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFL 256
           + YD+M L NGVLRLQPKGGH  HNG+K+V  HL+G R +PRL IGIGNPPG MDMKAFL
Sbjct: 138 MIYDDMGLSNGVLRLQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFL 197

Query: 257 LQKFSSVERKQIDTALEQGVEAVRTLALNGFSQRVNRFNLSQKYKYHKV 306
           LQKFS +ERKQ+D  LEQGVE V+TL   GFS  ++RFNL QKYK+HKV
Sbjct: 198 LQKFSPLERKQMDEGLEQGVEGVKTLVEEGFSDSISRFNLGQKYKFHKV 246

BLAST of Chy6G119500 vs. TAIR 10
Match: AT5G19830.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 251.5 bits (641), Expect = 8.5e-67
Identity = 116/197 (58.88%), Postives = 148/197 (75.13%), Query Frame = 0

Query: 108 YTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILL 167
           + PWL +GLGNPG+KY GTRHN+GFEMID  A++ GI MN +  KA++G G + ++P++L
Sbjct: 16  HRPWLFLGLGNPGDKYKGTRHNIGFEMIDVFAESVGIQMNLVNFKAIMGQGFVADLPVIL 75

Query: 168 AKPQTYMNFCGESVGPLAAHYQIPLRHVLLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVM 227
           AKPQTYMN  GES GPLAA+Y++PL  VL+ +D+M LP GVLRLQ KGGHG HNG+KSVM
Sbjct: 76  AKPQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPCGVLRLQEKGGHGCHNGLKSVM 135

Query: 228 GHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDTALEQGVEAVRTLALNGF 287
            H  G REF RL IGIG PPG MD KAFLLQKFS   R+++D AL +GV+A++ +    F
Sbjct: 136 NHFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPARERMDKALAEGVDALKLVLAKDF 195

Query: 288 SQRVNRFNLSQKYKYHK 305
            +    FN+ QKYK+ K
Sbjct: 196 GESWRLFNVEQKYKHLK 212

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LF141.4e-9873.91Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Ara... [more]
Q9FKN43.4e-9271.62Chloroplastic group IIB intron splicing facilitator CRS2-A, chloroplastic OS=Ara... [more]
Q9M5P42.7e-8966.27Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Zea m... [more]
Q5ZCL81.2e-8659.51Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Oryza... [more]
Q6NLS81.2e-6558.88Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g19... [more]
Match NameE-valueIdentityDescription
A0A0A0LCC62.3e-13697.98Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G257600 PE=3 SV=1[more]
A0A1S3CFG53.7e-13496.39chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Cuc... [more]
A0A6J1D2Z67.2e-12290.32chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic-like O... [more]
A0A6J1K6X41.2e-12189.92chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic-like O... [more]
A0A6J1G9M74.7e-12189.11chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic-like O... [more]
Match NameE-valueIdentityDescription
XP_004145630.11.99e-17497.98chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic [Cucum... [more]
XP_008461557.11.62e-17196.39PREDICTED: chloroplastic group IIB intron splicing facilitator CRS2-B, chloropla... [more]
XP_038899626.11.61e-16694.35chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic [Benin... [more]
KAG6607055.11.93e-15582.62Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic, parti... [more]
XP_022148094.12.12e-15590.32chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic-like [... [more]
Match NameE-valueIdentityDescription
AT5G16140.11.0e-9973.91Peptidyl-tRNA hydrolase family protein [more]
AT5G16140.21.0e-9973.91Peptidyl-tRNA hydrolase family protein [more]
AT5G38290.22.4e-9371.62Peptidyl-tRNA hydrolase family protein [more]
AT5G38290.12.4e-9371.62Peptidyl-tRNA hydrolase family protein [more]
AT5G19830.18.5e-6758.88Peptidyl-tRNA hydrolase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001328Peptidyl-tRNA hydrolaseTIGRFAMTIGR00447TIGR00447coord: 112..295
e-value: 4.7E-52
score: 174.4
IPR001328Peptidyl-tRNA hydrolasePFAMPF01195Pept_tRNA_hydrocoord: 112..295
e-value: 2.2E-54
score: 184.0
IPR001328Peptidyl-tRNA hydrolasePANTHERPTHR17224PEPTIDYL-TRNA HYDROLASEcoord: 76..304
IPR001328Peptidyl-tRNA hydrolaseHAMAPMF_00083Pept_tRNA_hydro_bactcoord: 110..288
score: 29.923649
IPR036416Peptidyl-tRNA hydrolase superfamilyGENE3D3.40.50.1470coord: 106..305
e-value: 1.8E-73
score: 248.3
IPR036416Peptidyl-tRNA hydrolase superfamilySUPERFAMILY53178Peptidyl-tRNA hydrolase-likecoord: 109..295
NoneNo IPR availablePANTHERPTHR17224:SF3CHLOROPLASTIC GROUP IIB INTRON SPLICING FACILITATOR CRS2-B, CHLOROPLASTICcoord: 76..304
NoneNo IPR availableCDDcd02406CRS2coord: 109..299
e-value: 1.47958E-131
score: 369.885
IPR018171Peptidyl-tRNA hydrolase, conserved sitePROSITEPS01196PEPT_TRNA_HYDROL_2coord: 216..226
IPR018171Peptidyl-tRNA hydrolase, conserved sitePROSITEPS01195PEPT_TRNA_HYDROL_1coord: 123..136

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy6G119500.1Chy6G119500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004045 aminoacyl-tRNA hydrolase activity