Chy3G056160 (gene) Cucumber (hystrix) v1

BLAST of Chy3G056160 vs. ExPASy Swiss-Prot
Match: Q0JL46 (Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 7.9e-226
Identity = 426/806 (52.85%), Postives = 544/806 (67.49%), Query Frame = 0

Query: 44  SSTWLKLMAMGTVLVAVSWCVNGD-WLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFR 103
           S  WL L+ +  VL+  S  ++   +LVG+GSFD+TGPAA+VNMMGYAN +Q  +GIHFR
Sbjct: 17  SRVWLWLL-LALVLLNCSLVLSASPYLVGMGSFDITGPAADVNMMGYANTEQIASGIHFR 76

Query: 104 LRARTFVVATSVDGPRIAFVNLDAGMASQLVTIKIFVQ---RFGDLYTEENVAISGIHTH 163
           L++R F+VA   +G R+ FVN+DA MASQ+VTIK+  +   R+GDLY E NVAISGIHTH
Sbjct: 77  LKSRAFIVA-EPNGKRVVFVNIDACMASQIVTIKVLERLKARYGDLYNENNVAISGIHTH 136

Query: 164 AGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKLG--------------- 223
           AGPGGYLQY+VY +TSLGFV+QSFD IV+ IEQSI++AH +L+ G               
Sbjct: 137 AGPGGYLQYVVYIVTSLGFVRQSFDVIVDGIEQSIVEAHNNLRPGKIFVNKGDLLDAGVN 196

Query: 224 -SPSAYLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLIS 283
            SPSAYL NP EER+KY  NVDKEM+++KFVD E G  +G+F+WF THGTSMSR N LIS
Sbjct: 197 RSPSAYLNNPAEERSKYEYNVDKEMTLIKFVDDELG-PVGSFNWFATHGTSMSRTNSLIS 256

Query: 284 GDNKGAAARFFEDWAAS------------------------NRRTTTTLSSNASGIEDLM 343
           GDNKGAAARF EDWA                           RR +T +       +DL+
Sbjct: 257 GDNKGAAARFMEDWAEQMGLPKQSAHANSDDLRSLHKTSVLPRRVSTIIPEPNEITDDLI 316

Query: 344 KKAEKIKATGGKRCRKTS-SKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNP 403
           + A   +A+GG+R   +S ++  +  + +   FV AFCQSN GDV+PNVLG FC D+  P
Sbjct: 317 QLASSYEASGGRRLAGSSITRRIRSTQQNKPKFVSAFCQSNCGDVSPNVLGTFCIDTNLP 376

Query: 404 CDFNHSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHV 463
           CDFNHS+C+G + LC GRGPG+PDE  ST++IG RQFLKA DLF +A+E++ G+ID+RH 
Sbjct: 377 CDFNHSTCNGKNELCYGRGPGYPDEFESTRVIGNRQFLKARDLFDSASEEIQGKIDYRHT 436

Query: 464 YLNFTDIEVEID----GNNVVRTCPAALGPGFAAGTTDGPGAFGFQQGDTEVSTMESEMN 523
           YL+F+ +EV++     G   V+TCPAA+G  FAAGTTDGPGAF F+QGD           
Sbjct: 437 YLDFSKLEVKVSTSAGGQQTVKTCPAAMGFAFAAGTTDGPGAFDFRQGD----------- 496

Query: 524 PCLSFFDLSLNFVLEILLQINKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAW 583
                            ++ N  WK +R+ LK P + QV C  PK +LLDTGEM EPY W
Sbjct: 497 -----------------VKGNPFWKLVRNLLKTPGKDQVECHSPKPILLDTGEMKEPYDW 556

Query: 584 APAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLT 643
           APAILP+Q++R+G+L+IL VPGEFTTMAGRRLR+AVK T++++GN EFD + HVV+AGLT
Sbjct: 557 APAILPVQMIRIGQLVILCVPGEFTTMAGRRLRDAVK-TVLTSGNSEFDKNIHVVLAGLT 616

Query: 644 NTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIASPPDL 703
           N+YSQY+ TFEEY+ QRYE ASTLYGPHTLSAYIQEF+KLA+A+   +++      PPD+
Sbjct: 617 NSYSQYITTFEEYQIQRYEGASTLYGPHTLSAYIQEFQKLAMAMIANKEVPTNF-QPPDM 676

Query: 704 SSVQLRLVMDPFGEPPPNGVNFGDIQQDVKLPKDGWFKTGSKQKPTATFWSANPRFDLLT 763
              Q+ L+     +  P GV FGD+  DV  P +  F  GS     ATF+SA PR DLLT
Sbjct: 677 LDKQIGLLPGVVFDSTPLGVKFGDVNSDV--PGNSTFNKGS--TVNATFYSACPRNDLLT 736

Query: 764 EGTYALVERLE-KQRWTPAYDDDDFSLFFKWKLDNTFIN-SLVTIEWDIPVDTDPGVYRL 799
           +GT+ALVE+L+    W P YDDDD+SL FKW       + S  T+EW +P D   GVYRL
Sbjct: 737 DGTFALVEKLDGNNNWVPVYDDDDWSLRFKWSRPARLSSRSFATLEWTVPEDAAAGVYRL 784

BLAST of Chy3G056160 vs. ExPASy Swiss-Prot
Match: Q304B9 (Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 6.7e-225
Identity = 423/770 (54.94%), Postives = 526/770 (68.31%), Query Frame = 0

Query: 68  WLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVATSVDGPRIAFVNLDAG 127
           +L+GVGS+D+TGPAA+VNMMGYAN DQ  +GIHFRLRAR F+VA    G R+ FVNLDA 
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVA-EPQGNRVVFVNLDAC 85

Query: 128 MASQLVTIKIFVQ---RFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFD 187
           MASQ+VTIK+  +   R+G+LYTE+NVAISGIHTHAGPGGYLQY+ Y +TSLGFV+QSFD
Sbjct: 86  MASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFD 145

Query: 188 AIVNAIEQSIIQAHESLKLG----------------SPSAYLMNPEEERAKYANNVDKEM 247
            +VN IEQSI+QAHESL+ G                SPS+YL NP  ER+KY  +VDKEM
Sbjct: 146 VVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEM 205

Query: 248 SILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWAASN------- 307
           +++KFVD + G + G+F+WF THGTSMSR N LISGDNKGAAARF EDW  +        
Sbjct: 206 TLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSS 265

Query: 308 ----RRTTTTLSSNASGIEDLMKKAEKIKATGGKRCRKTSSKSSKVRKNDGSLFVGAFCQ 367
               RR +T +S  +   + L+  A   K++ G    K+    ++VR      FV AFCQ
Sbjct: 266 RNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQ 325

Query: 368 SNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLK 427
           SN GDV+PN LG FC D+G PCDFNHS+C+G + LC GRGPG+PDE  ST+IIGE+QF  
Sbjct: 326 SNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKM 385

Query: 428 AADLFTTATEKLTGEIDFRHVYLNFTDIEVEI----DGNNVVRTCPAALGPGFAAGTTDG 487
           A +LF  ATEKL G+I ++H YL+F++++V +     G+  V+TCPAA+G GFAAGTTDG
Sbjct: 386 AVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDG 445

Query: 488 PGAFGFQQGDTEVSTMESEMNPCLSFFDLSLNFVLEILLQINKLWKQLRDSLKKPSEFQV 547
           PGAF F+QGD                             Q N  W+ +R+ L+ P   QV
Sbjct: 446 PGAFDFKQGDD----------------------------QGNVFWRLVRNVLRTPGPEQV 505

Query: 548 GCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKET 607
            CQKPK +LLDTGEM EPY WAP+ILPIQILR+G+L+ILSVPGEFTTMAGRRLR+A+K  
Sbjct: 506 QCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSF 565

Query: 608 LISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKK 667
           LIS+   EF ++ HVVIAGLTNTYSQY+ATFEEYE QRYE ASTLYG HTL+AYIQEFKK
Sbjct: 566 LISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKK 625

Query: 668 LAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGEPPPNGVNFGDIQQDVKLPKDGWFKT 727
           LA A+  G  + +    PPDL   Q+ L+     +  P GV FGD++ DV  P    F+ 
Sbjct: 626 LATALVNGLTLPRG-PQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADV--PPKSTFRR 685

Query: 728 GSKQKPTATFWSANPRFDLLTEGTYALVERL-EKQRWTPAYDDDDFSLFFKW----KLDN 787
           G  Q+  ATFWS  PR DL+TEG++A+VE L E  +W P YDDDDFSL FKW    KL +
Sbjct: 686 G--QQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSS 745

Query: 788 TFINSLVTIEWDIPVDTDPGVYRLRHFGSSRSTINSTNIYFTGASRGFAV 799
               S  TIEW +P     GVYR+RH+G+S+S   S +  F+G+S  F V
Sbjct: 746 ---ESQATIEWRVPESAVAGVYRIRHYGASKSLFGSIS-SFSGSSSAFVV 756

BLAST of Chy3G056160 vs. ExPASy Swiss-Prot
Match: F4HQM3 (Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 4.6e-218
Identity = 411/780 (52.69%), Postives = 524/780 (67.18%), Query Frame = 0

Query: 65  NGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVATSVDGPRIAFVNL 124
           + ++L+G+GS+D+TGPAA+VNMMGYANM+Q  +GIHFRLRARTF+V +   G R+ FVNL
Sbjct: 34  HSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIV-SEPQGKRVVFVNL 93

Query: 125 DAGMASQLVTIKIFVQ---RFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQ 184
           DA MASQ+V +K+  +   R+GDLYTE+NV ISGIHTHAGPGGYLQY+VY +TSLGFV+Q
Sbjct: 94  DACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQ 153

Query: 185 SFDAIVNAIEQSIIQAHESLKLG----------------SPSAYLMNPEEERAKYANNVD 244
           SFDA+V+ IE SIIQAHE+L+ G                SPSAYL NP +ER+K+  NVD
Sbjct: 154 SFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNVD 213

Query: 245 KEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWAASN---- 304
           KEM++LKFVD + G  +G+F+WF THGTSMSR N LISGDNKGAA+RF EDW   N    
Sbjct: 214 KEMTLLKFVDDQWG-PVGSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAER 273

Query: 305 ------------RRTTTTLSSNASGIEDLMKKAEKIKATGGKRCRKTSSKSSKV----RK 364
                       RR ++ + ++     +L++ A   ++  GK   + SS + +V    RK
Sbjct: 274 SYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRISSSARRVRSALRK 333

Query: 365 NDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEIL 424
            D   FV AFCQ+N GDV+PNVLGAFC D+G PCDFNHS+C G + +C GRGPG+PDE  
Sbjct: 334 ADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEFE 393

Query: 425 STKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEI---DG-NNVVRTCPAA 484
           ST+IIGERQF  A +LF  A+E+L G++D+RHVY++F+ + V +   DG + VV+TCPAA
Sbjct: 394 STRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAA 453

Query: 485 LGPGFAAGTTDGPGAFGFQQGDTEVSTMESEMNPCLSFFDLSLNFVLEILLQINKLWKQL 544
           +G  FAAGTTDGPGAF F QGD +                             N  W+ +
Sbjct: 454 MGFAFAAGTTDGPGAFDFTQGDDKG----------------------------NPFWRLV 513

Query: 545 RDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTM 604
           R+ LK P + Q+ C  PK +LLDTGEM +PY WAP+IL +Q+LR+G+L ILSVPGEFTTM
Sbjct: 514 RNVLKTPDKKQIDCHYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTM 573

Query: 605 AGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGP 664
           AGRRLR AVK  L ++GN +   + HVVIAGL N YSQYV TFEEY+ QRYE ASTLYGP
Sbjct: 574 AGRRLRYAVKTQLKNSGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGP 633

Query: 665 HTLSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGEPPPNGVNFGDIQQ 724
           HTLS YIQEFKKL+ ++     + +P   PPDL   QL  +     +  P+G +FGD+  
Sbjct: 634 HTLSGYIQEFKKLSKSLVLDMPV-QPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVIS 693

Query: 725 DVKLPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEK--QRWTPAYDDDDFS 784
           DV  PK+   K G+ Q  T  F SA PR DLLTEGT+ LVERLE+  + WTP YDDDD  
Sbjct: 694 DV--PKNLSLKRGNGQ-VTVVFRSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLC 753

Query: 785 LFFKWKLDNTFIN-SLVTIEWDIPVDTDPGVYRLRHFGSSRSTINSTNIYFTGASRGFAV 799
           L FKW       + S  T+EW IP    PGVYR+ HFG+++    S + +FTG+S  F V
Sbjct: 754 LRFKWSRHKKLSSRSQATVEWRIPESASPGVYRITHFGAAKKLFGSVH-HFTGSSSAFVV 778

BLAST of Chy3G056160 vs. ExPASy Swiss-Prot
Match: F4KHQ8 (Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 4.8e-207
Identity = 406/776 (52.32%), Postives = 505/776 (65.08%), Query Frame = 0

Query: 65  NGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVATSVDGPRIAFVNL 124
           + D+L+G+GS+D+TGPAA+VNMMGYANM+Q  +G+HFRLRAR F+VA      RIAFVNL
Sbjct: 26  DSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFIVAEPYK-KRIAFVNL 85

Query: 125 DAGMASQLVTIKI---FVQRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQ 184
           DAGMASQLVTIK+     QR+G+LYTEENVAISG HTHAGPGGYLQY++Y +TSLGFV Q
Sbjct: 86  DAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYLQYILYLVTSLGFVHQ 145

Query: 185 SFDAIVNAIEQSIIQAHESLKLG----------------SPSAYLMNPEEERAKYANNVD 244
           SF+A+V+ IEQSIIQAHE+L+ G                SPSAYL NP  ER+KY  +VD
Sbjct: 146 SFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYLNNPAHERSKYEYDVD 205

Query: 245 KEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDW-------- 304
           KEM+++KFVD                             D  G  AR  EDW        
Sbjct: 206 KEMTLVKFVD-----------------------------DQWGPVARIMEDWFERENGCR 265

Query: 305 ---AASNRRTTTTLSSNASGIEDLMKKAEKIKATGGKRCRKTSSKSSKVRKN----DGSL 364
                S RR ++ +S      +DLM+ A  + +TGGK   + SS + +VR      D   
Sbjct: 266 SVDVESPRRVSSIISDPYD--QDLMEMASSLLSTGGKTVTRMSSVARRVRSRFRHADKPR 325

Query: 365 FVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTKII 424
           FV AFCQ+N GDV+PNVLGAFC D+G PC+FN S+C G +  C GRGPG+PDE  ST+II
Sbjct: 326 FVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNEQCYGRGPGYPDEFESTRII 385

Query: 425 GERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNN----VVRTCPAALGPGF 484
           GERQF KAADLFT A+E++ G++D+RH Y++F+ +EV I+G N    VV+TCPAA+G GF
Sbjct: 386 GERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAMGFGF 445

Query: 485 AAGTTDGPGAFGFQQGDTEVSTMESEMNPCLSFFDLSLNFVLEILLQINKLWKQLRDSLK 544
           AAGTTDGPGAF F+QGD                             Q N  W+ +R+ LK
Sbjct: 446 AAGTTDGPGAFDFKQGDD----------------------------QGNPFWRLVRNLLK 505

Query: 545 KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRL 604
            P+E QV CQ+PK +LLDTGEM +PY WAP+ILP+QILR+G+L+IL VPGEFTTMAGRRL
Sbjct: 506 NPTEEQVRCQRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRL 565

Query: 605 REAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSA 664
           R+AVK  L    NG    +  VVIAGLTN+YSQY+ATFEEY+ QRYE ASTLYGPHTLS 
Sbjct: 566 RDAVKTVLKEGSNGR---EFSVVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSG 625

Query: 665 YIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGEPPPNGVNFGDIQQDVKLP 724
           YIQEFKKLA  +   +    P   PPDL   Q+ L+     +  P G  FGD+  DV  P
Sbjct: 626 YIQEFKKLANDLLSAQ-TTDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDV--P 685

Query: 725 KDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVER-LE-KQRWTPAYDDDDFSLFFKW 784
           +   F+ G+       F SANPR DL+TEGT+ALVER LE ++ W P YDDDDF L FKW
Sbjct: 686 RLSKFRKGA-DIVRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKW 733

Query: 785 KLD-NTFINSLVTIEWDIPVDTDPGVYRLRHFGSSRSTINSTNIYFTGASRGFAVF 800
                    S  TIEW IP    PGVYR+ HFGS+++ I+S + +F+G+S  F V+
Sbjct: 746 SRPFKLSTQSTATIEWRIPETASPGVYRITHFGSAKTPISSIH-HFSGSSSAFVVY 733

BLAST of Chy3G056160 vs. ExPASy Swiss-Prot
Match: Q55G11 (Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 4.3e-139
Identity = 305/761 (40.08%), Postives = 425/761 (55.85%), Query Frame = 0

Query: 65  NGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVATSVDGPRIAFVNL 124
           +  + +G G +D+TG +AEVN+MGYAN  Q  AGIHFR RAR FV   S +G R  +V+ 
Sbjct: 43  DSSYQIGAGIYDITGASAEVNLMGYANPLQVGAGIHFRQRARAFVFVDS-NGNRAVYVST 102

Query: 125 DAGMASQLV---TIKIFVQRFG-DLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQ 184
           D+ M  Q V    +++    FG ++YTE NV +SG HTH+GP G+ QY +Y ITSLGF +
Sbjct: 103 DSCMIFQEVKIHVVELLQDIFGPNVYTEANVLLSGTHTHSGPAGFSQYALYGITSLGFYK 162

Query: 185 QSFDAIVNAIEQSIIQAHESLKLG----------------SPSAYLMNPEEERAKYANNV 244
           ++FD I N I Q+I++AH+S++                  SP AY  NPEEE+A Y +NV
Sbjct: 163 KNFDTICNGIVQAIVKAHKSVQPANMFTETGELWNTNINRSPFAYDNNPEEEKAMYDSNV 222

Query: 245 DKEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWAASNRRT 304
           DK M++L+ ++  NGN   A S+F  H TSM+  N LISGDNKG A+  +E         
Sbjct: 223 DKNMTVLR-IEDMNGNPFAAISFFAVHCTSMNNTNHLISGDNKGYASYLWE--------- 282

Query: 305 TTTLSSNASGIEDLMKKAEKIKATGGKRCRKTSSKSSKVRKNDGSLFVGAFCQSNVGDVT 364
                           K      T GK                   FV AF QSN GDV+
Sbjct: 283 ----------------KQVNGPGTAGK-----------------GPFVAAFGQSNEGDVS 342

Query: 365 PNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEIL-STKIIGERQFLKAADLFT 424
           PN  G  C D G+PCD+  S+C+G +  C   GPG   ++  ST+IIG  QF KA +LF 
Sbjct: 343 PNTRGPTCRD-GSPCDYKTSTCNGRNEECWSLGPGKDGDMFESTQIIGGNQFNKALELFN 402

Query: 425 TATEKLTGEIDFRHVYLNFTDIEVEIDGNNVV---RTCPAALGPGFAAGTTDGPGAFGFQ 484
            A+ +++G + +RH ++ FT++ VE   N+ V    TC  A+G  FAAGTTDGPGAF F 
Sbjct: 403 NASIQVSGPVQYRHSWVQFTNVSVEPPYNSGVDNATTCRGAMGYSFAAGTTDGPGAFNFV 462

Query: 485 QGDTEVSTMESEMNPCLSFFDLSLNFVLEILLQINKLWKQLRDSLKKPSEFQVGCQKPKT 544
           Q D   S                           N  W  + D + KP+  Q+ CQ PK 
Sbjct: 463 QSDNNTSG--------------------------NPFWNFIGDFIAKPTPDQIRCQSPKP 522

Query: 545 VLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNG 604
           +LLD G M EP  W P ++PIQI+ +G++++++VPGEFTTM+GRRLR +V+E +     G
Sbjct: 523 ILLDVG-MVEPIPWVPDVMPIQIVTIGQIVLVAVPGEFTTMSGRRLRNSVREII-----G 582

Query: 605 EFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAK 664
           E  ++  V+IAGL+NTYS Y+ATFEEY+ QRYE AST++GPHTL +Y+QEF KLA +I  
Sbjct: 583 ESIENPIVLIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHTLGSYMQEFGKLAQSIVD 642

Query: 665 GEKI-AKPIASPPDLSSVQLRLVMDPFGEPPPNGVNFGDIQQDVKLPKDGWFKTGSKQKP 724
           G  + A P  +P +L+   L  +     +  P+  +FG++  DV L           +  
Sbjct: 643 GTTVPAGP--TPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDVNL------NYSVNETV 702

Query: 725 TATFWSANPRFDLLTEGTYALVERLE-KQRWTPAYDDDDFSLFFKWKLDNTFINSLVTIE 784
           +  F+  NPR D + E ++  V+ L    +WT   DD D+   FKWK+ +    SL+TIE
Sbjct: 703 SCVFYGGNPRNDFMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWKMHDLGF-SLITIE 717

Query: 785 WDIPVDTDPGVYRLRHFGSSRSTINSTNIY-FTGASRGFAV 799
           W I  DT PG YR+ H G ++    S+N+  + G SR F V
Sbjct: 763 WVIAPDTTPGTYRITHSGFAKKNPFSSNLTPYQGISRNFVV 717

BLAST of Chy3G056160 vs. ExPASy TrEMBL
Match: A0A1S3CMI6 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103502652 PE=3 SV=1)

HSP 1 Score: 1342.4 bits (3473), Expect = 0.0e+00
Identity = 683/776 (88.02%), Postives = 696/776 (89.69%), Query Frame = 0

Query: 53  MGTVLVAVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVAT 112
           M  V++ VSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQ TAGIHFRLRARTFVVA 
Sbjct: 1   MAIVVLDVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQVTAGIHFRLRARTFVVAR 60

Query: 113 SVDGPRIAFVNLDAGMASQLVTIKIF------------VQRFGDLYTEENVAISGIHTHA 172
           S+DGPRIAFVNLDAGMASQLVTIK+             VQRFGDLYTEENVAISGIHTHA
Sbjct: 61  SIDGPRIAFVNLDAGMASQLVTIKVLERLQSRRNINISVQRFGDLYTEENVAISGIHTHA 120

Query: 173 GPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKLG---------------- 232
           GPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLK G                
Sbjct: 121 GPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGEVDNAGINR 180

Query: 233 SPSAYLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISG 292
           SPSAYLMNPEEERAKYANNVDKEMSILKFVDGE+GNSIGAFSWFPTHGTSMSRDNKLISG
Sbjct: 181 SPSAYLMNPEEERAKYANNVDKEMSILKFVDGESGNSIGAFSWFPTHGTSMSRDNKLISG 240

Query: 293 DNKGAAARFFEDWAASNRRTTTTLSSNASGIEDLMKKAEKIKATGGKRCRKTSSKSSKVR 352
           DNKGAAARFFEDWAASN RTTTT S+NAS IE+LMKKAEKIKATGGK+C KTSSKSSKVR
Sbjct: 241 DNKGAAARFFEDWAASNGRTTTTPSNNASVIEELMKKAEKIKATGGKQCSKTSSKSSKVR 300

Query: 353 KNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEI 412
           KNDGSLFV AFCQSNVGDVTPNVLGAFC DSG PCDFN SSCHGSDLLCVGRGPGFPDEI
Sbjct: 301 KNDGSLFVAAFCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEI 360

Query: 413 LSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALGP 472
           LSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALGP
Sbjct: 361 LSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALGP 420

Query: 473 GFAAGTTDGPGAFGFQQGDTEVSTMESEMNPCLSFFDLSLNFVLEILLQINKLWKQLRDS 532
           GFAAGTTDGPG FGFQQGDTE                            INKLWKQLRDS
Sbjct: 421 GFAAGTTDGPGVFGFQQGDTE----------------------------INKLWKQLRDS 480

Query: 533 LKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGR 592
           LKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGR
Sbjct: 481 LKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGR 540

Query: 593 RLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTL 652
           RLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTL
Sbjct: 541 RLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTL 600

Query: 653 SAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGEPPPNGVNFGDIQQDVK 712
           SAYIQEFKKLA AIAKGEK+AKPIASPPDLSSVQLRLVMDPFGE PP+GVNFGDIQQDV+
Sbjct: 601 SAYIQEFKKLASAIAKGEKVAKPIASPPDLSSVQLRLVMDPFGESPPDGVNFGDIQQDVE 660

Query: 713 LPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSLFFKW 772
           LPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFS+ FKW
Sbjct: 661 LPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSVSFKW 720

Query: 773 KLDN-TFINSLVTIEWDIPVDTDPGVYRLRHFGSSRSTINSTNIYFTGASRGFAVF 800
           KLDN T INSL TIEWDIP+D DPGVYRLRHFGSSRSTINSTNIYFTGASR FAVF
Sbjct: 721 KLDNTTLINSLATIEWDIPIDADPGVYRLRHFGSSRSTINSTNIYFTGASRAFAVF 748

BLAST of Chy3G056160 vs. ExPASy TrEMBL
Match: A0A0A0KII3 (Neutral ceramidase OS=Cucumis sativus OX=3659 GN=Csa_6G361230 PE=3 SV=1)

HSP 1 Score: 1216.1 bits (3145), Expect = 0.0e+00
Identity = 613/687 (89.23%), Postives = 621/687 (90.39%), Query Frame = 0

Query: 139 VQRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAH 198
           ++ FGD+YTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAH
Sbjct: 1   MKTFGDVYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAH 60

Query: 199 ESLKLG--------------------------SPSAYLMNPEEERAKYANNVDKEMSILK 258
           ESLK G                          SPSAYLMNPEEERAKYANNVDKEMSILK
Sbjct: 61  ESLKPGFILFNKGKQIRQLFHFWEVENAGINRSPSAYLMNPEEERAKYANNVDKEMSILK 120

Query: 259 FVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWAASNRRTTTTLSSNA 318
           FVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWAASNRRTTTT S+N 
Sbjct: 121 FVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWAASNRRTTTTPSNNT 180

Query: 319 SGIEDLMKKAEKIKATGGKRCRKTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFC 378
           SGIEDLMKKA KIKATGGKRC KTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFC
Sbjct: 181 SGIEDLMKKAGKIKATGGKRCSKTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFC 240

Query: 379 TDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGE 438
           TDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGE
Sbjct: 241 TDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGE 300

Query: 439 IDFRHVYLNFTDIEVEIDGNNVVRTCPAALGPGFAAGTTDGPGAFGFQQGDTEVSTMESE 498
           IDFRHVYLNFTDIEV IDGNNVVRTCPAALGPGFAAGTTDGPGAFGFQQGDTE       
Sbjct: 301 IDFRHVYLNFTDIEVAIDGNNVVRTCPAALGPGFAAGTTDGPGAFGFQQGDTE------- 360

Query: 499 MNPCLSFFDLSLNFVLEILLQINKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPY 558
                                INKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPY
Sbjct: 361 ---------------------INKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPY 420

Query: 559 AWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAG 618
           AWAPAILPIQILRLG LIILSVPGEFTTMAGRRLREAVKETLISNGNG FDDDTHVVIAG
Sbjct: 421 AWAPAILPIQILRLGNLIILSVPGEFTTMAGRRLREAVKETLISNGNGVFDDDTHVVIAG 480

Query: 619 LTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIASPP 678
           LTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIASPP
Sbjct: 481 LTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIASPP 540

Query: 679 DLSSVQLRLVMDPFGEPPPNGVNFGDIQQDVKLPKDGWFKTGSKQKPTATFWSANPRFDL 738
           DLSSVQLRLVMDPFGE PPNGVNFGDIQQDV+LPKDGWFKTGSKQKPTATFWSANPRFDL
Sbjct: 541 DLSSVQLRLVMDPFGESPPNGVNFGDIQQDVELPKDGWFKTGSKQKPTATFWSANPRFDL 600

Query: 739 LTEGTYALVERLEKQRWTPAYDDDDFSLFFKWKLDNTFINSLVTIEWDIPVDTDPGVYRL 798
           LTEGTYALVERLEKQRWTPAYDDDDFSLFFKWKLDNTFIN+LVTIEWDIP+D DPGVYRL
Sbjct: 601 LTEGTYALVERLEKQRWTPAYDDDDFSLFFKWKLDNTFINNLVTIEWDIPIDADPGVYRL 659

Query: 799 RHFGSSRSTINSTNIYFTGASRGFAVF 800
           RHFGSSRSTINSTN+YFTGASR FAVF
Sbjct: 661 RHFGSSRSTINSTNVYFTGASRAFAVF 659

BLAST of Chy3G056160 vs. ExPASy TrEMBL
Match: A0A5A7TA71 (Neutral ceramidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold119G00340 PE=3 SV=1)

HSP 1 Score: 1206.4 bits (3120), Expect = 0.0e+00
Identity = 628/777 (80.82%), Postives = 642/777 (82.63%), Query Frame = 0

Query: 40  MWRH-SSTWLKLMAMGTVLVAVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAG 99
           M RH SS+WLK MAM  V++ VSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQ TAG
Sbjct: 1   MRRHNSSSWLKFMAMAIVVLDVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQVTAG 60

Query: 100 IHFRLRARTFVVATSVDGPRIAFVNLDAGMASQLVTIKIFVQRFGDLYTEENVAISGIHT 159
           IHFRLRARTFVVA                                       V  +GI+ 
Sbjct: 61  IHFRLRARTFVVA------------------------------------RREVDNAGINR 120

Query: 160 HAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKLGSPSAYLMNPEEERA 219
                                                         SPSAYLMNPEEERA
Sbjct: 121 ----------------------------------------------SPSAYLMNPEEERA 180

Query: 220 KYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWA 279
           KYANNVDKEMSILKFVDGE+GNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWA
Sbjct: 181 KYANNVDKEMSILKFVDGESGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWA 240

Query: 280 ASNRRTTTTLSSNASG---------------IEDLMKKAEKIKATGGKRCRKTSSKSSKV 339
           ASN RTTTT S+NAS                IE+LMKKAEKIKATGGKRC KTSSKSSKV
Sbjct: 241 ASNGRTTTTPSNNASENLYNFLFLVYILKTVIEELMKKAEKIKATGGKRCSKTSSKSSKV 300

Query: 340 RKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDE 399
           RKNDGSLFV AFCQSNVGDVTPNVLGAFCTDSG PCDFN SSCHGSDLLCVGRGPGFPDE
Sbjct: 301 RKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDE 360

Query: 400 ILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALG 459
           ILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALG
Sbjct: 361 ILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALG 420

Query: 460 PGFAAGTTDGPGAFGFQQGDTEVSTMESEMNPCLSFFDLSLNFVLEILLQINKLWKQLRD 519
           PGFAAGTTDGPG FGFQQGDTEVS+M   MN CLSFFD  LNF   ILLQINKLWKQLRD
Sbjct: 421 PGFAAGTTDGPGVFGFQQGDTEVSSMADGMNLCLSFFD-CLNFGFAILLQINKLWKQLRD 480

Query: 520 SLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAG 579
           SLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAG
Sbjct: 481 SLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAG 540

Query: 580 RRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHT 639
           RRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHT
Sbjct: 541 RRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHT 600

Query: 640 LSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGEPPPNGVNFGDIQQDV 699
           LSAYIQEFKKLA AIAKGEK+AKPIASPPDLSSVQLRLVMDPFGE PP+GVNFGDIQQDV
Sbjct: 601 LSAYIQEFKKLASAIAKGEKVAKPIASPPDLSSVQLRLVMDPFGESPPDGVNFGDIQQDV 660

Query: 700 KLPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSLFFK 759
           +LPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFS+ FK
Sbjct: 661 ELPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSVSFK 694

Query: 760 WKLDN-TFINSLVTIEWDIPVDTDPGVYRLRHFGSSRSTINSTNIYFTGASRGFAVF 800
           WKLDN T INSL TIEWDIP+D DPGVYRLRHFGSSRSTINSTNIYFTGASR FAVF
Sbjct: 721 WKLDNTTLINSLATIEWDIPIDADPGVYRLRHFGSSRSTINSTNIYFTGASRAFAVF 694

BLAST of Chy3G056160 vs. ExPASy TrEMBL
Match: A0A251R941 (Neutral ceramidase OS=Prunus persica OX=3760 GN=PRUPE_1G356500 PE=3 SV=1)

HSP 1 Score: 1104.0 bits (2854), Expect = 0.0e+00
Identity = 561/781 (71.83%), Postives = 638/781 (81.69%), Query Frame = 0

Query: 39  IMWRHSSTWLKLMAMGTVLVAVSWCVNGD-WLVGVGSFDMTGPAAEVNMMGYANMDQNTA 98
           I  +HSS +  L+ M  V + V    NG+ +L+GVGS+DMTGPAAEVNMMGYANMDQ+TA
Sbjct: 7   IQEQHSSLFSSLIIMIIVGIGVIRRCNGEGYLIGVGSYDMTGPAAEVNMMGYANMDQSTA 66

Query: 99  GIHFRLRARTFVVATSVDGPRIAFVNLDAGMASQLVTIKI---FVQRFGDLYTEENVAIS 158
           G+HFRLRARTF+VA S  GPR AFVNLDAGMASQLV IK+      RFGDLYTEENVAIS
Sbjct: 67  GVHFRLRARTFIVAESSQGPRFAFVNLDAGMASQLVNIKVLDKLKSRFGDLYTEENVAIS 126

Query: 159 GIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKLG---------- 218
           GIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSI+QAH +LK G          
Sbjct: 127 GIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHHNLKPGSVFINEGDVV 186

Query: 219 ------SPSAYLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSMSRD 278
                 SPSAYL+NP EERA+Y +NVD  M++LKFVD  +G S+GAFSWF THGTSMS++
Sbjct: 187 NAGINRSPSAYLLNPAEERARYPSNVDTLMTLLKFVDDASGKSVGAFSWFATHGTSMSKN 246

Query: 279 NKLISGDNKGAAARFFEDWAASNRRTTTTLSSNASGIEDLMKKAEKIKATGGKRCRKTSS 338
           N LISGDNKGAAARFFEDW +S+  ++TT + +++ +  L+KKA+ +KATGGK C KTSS
Sbjct: 247 NLLISGDNKGAAARFFEDWFSSSSSSSTTSADSSASLLSLIKKAQSMKATGGKLCGKTSS 306

Query: 339 KSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGP 398
           + SKVRKN+GSLFVGAFCQSNVGDVTPNVLGAFC DSG PCDFNHSSCHG+DLLC+GRGP
Sbjct: 307 RESKVRKNNGSLFVGAFCQSNVGDVTPNVLGAFCADSGRPCDFNHSSCHGNDLLCLGRGP 366

Query: 399 GFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVE-IDGNNVVRT 458
           G+PDEILSTKIIGERQF KAA LFTTAT++LTG ID+RHVYLNFTDIEVE +DGN  V+T
Sbjct: 367 GYPDEILSTKIIGERQFQKAAHLFTTATQQLTGTIDYRHVYLNFTDIEVELLDGNKKVKT 426

Query: 459 CPAALGPGFAAGTTDGPGAFGFQQGDTEVSTMESEMNPCLSFFDLSLNFVLEILLQINKL 518
           CPAALGPGFAAGTTDGPGAFGFQQGDT                            +INKL
Sbjct: 427 CPAALGPGFAAGTTDGPGAFGFQQGDT----------------------------KINKL 486

Query: 519 WKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGE 578
           W++LRD+LKKP+ +QV CQKPKTVLLDTGEMF PYAWAPAILPIQ+LRLGKLIILSVPGE
Sbjct: 487 WRKLRDALKKPTPYQVDCQKPKTVLLDTGEMFVPYAWAPAILPIQMLRLGKLIILSVPGE 546

Query: 579 FTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAST 638
           FTTMAGRRLREAVKETLISN NGEFDD+TH+VIAGLTNTYSQY+ATFEEY QQRYEAAST
Sbjct: 547 FTTMAGRRLREAVKETLISNSNGEFDDETHIVIAGLTNTYSQYIATFEEYAQQRYEAAST 606

Query: 639 LYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGEPPPNGVNFG 698
           LYGPHTLSAYIQEF+KLA A+AKGE+I K   SPPDLSSVQLRL+++P G+ PP   NFG
Sbjct: 607 LYGPHTLSAYIQEFEKLAKAMAKGEQITKG-PSPPDLSSVQLRLLLEPTGDLPPPHTNFG 666

Query: 699 DIQQDVKLPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDD 758
           D++QD+ +PK G FK G   +P ATFWSANPR+DLLTEGT+A+VE L+ + W P YDDDD
Sbjct: 667 DMKQDIIIPKSGSFKKG--DRPIATFWSANPRYDLLTEGTFAVVELLQGKHWVPVYDDDD 726

Query: 759 FSLFFKWKLDNTFINSLVTIEWDIPVDTDPGVYRLRHFGSSRSTINSTNIYFTGASRGFA 799
           F LFFKWK+DN  +    TIEW+IP D D GVYRLRHFGSS+ T +S NIYFTGAS GFA
Sbjct: 727 FCLFFKWKVDNITLYGTATIEWEIPRDADSGVYRLRHFGSSKKTKDSPNIYFTGASSGFA 756

BLAST of Chy3G056160 vs. ExPASy TrEMBL
Match: A0A5E4FD18 (Neutral ceramidase OS=Prunus dulcis OX=3755 GN=ALMOND_2B003982 PE=3 SV=1)

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 561/781 (71.83%), Postives = 637/781 (81.56%), Query Frame = 0

Query: 39  IMWRHSSTWLKLMAMGTVLVAVSWCVNGD-WLVGVGSFDMTGPAAEVNMMGYANMDQNTA 98
           I  +HSS +  L+ M  V + V    NG+ +L+GVGS+DMTGPAA VNMMGYANMDQ+TA
Sbjct: 7   IQEQHSSLFSSLIIMIIVGIGVIRRCNGEGYLIGVGSYDMTGPAAGVNMMGYANMDQSTA 66

Query: 99  GIHFRLRARTFVVATSVDGPRIAFVNLDAGMASQLVTIKI---FVQRFGDLYTEENVAIS 158
           G+HFRLRARTF+VA S  GPR AFVNLDAGMASQLV IK+      RFGDLYTEENVAIS
Sbjct: 67  GVHFRLRARTFIVAESSQGPRFAFVNLDAGMASQLVNIKVLDKLKSRFGDLYTEENVAIS 126

Query: 159 GIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKLG---------- 218
           GIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSI+QAH +LK            
Sbjct: 127 GIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHHNLKPASVFINEGDVV 186

Query: 219 ------SPSAYLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSMSRD 278
                 SPSAYL+NP EERA+Y +NVD  M++LKFVD  +G S+GAFSWF THGTSMS++
Sbjct: 187 NAGINRSPSAYLLNPAEERARYPSNVDTLMTLLKFVDDASGKSVGAFSWFATHGTSMSKN 246

Query: 279 NKLISGDNKGAAARFFEDWAASNRRTTTTLSSNASGIEDLMKKAEKIKATGGKRCRKTSS 338
           N LISGDNKGAAARFFEDW +S+  ++TT + +++ +  L+KKA+ +KATGGK C KTSS
Sbjct: 247 NLLISGDNKGAAARFFEDWFSSSSSSSTTSADSSASLLSLIKKAQSMKATGGKLCGKTSS 306

Query: 339 KSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGP 398
           + SKVRKN+GSLFVGAFCQSNVGDVTPNVLGAFCTDSG PCDFNHSSCHG+DLLC+GRGP
Sbjct: 307 RESKVRKNNGSLFVGAFCQSNVGDVTPNVLGAFCTDSGRPCDFNHSSCHGNDLLCLGRGP 366

Query: 399 GFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVE-IDGNNVVRT 458
           G+PDEILSTKIIGERQF KAA LFTTAT++LTG ID+RHVYLNFTDIEVE +DGN  V+T
Sbjct: 367 GYPDEILSTKIIGERQFQKAAHLFTTATQQLTGTIDYRHVYLNFTDIEVELLDGNKKVKT 426

Query: 459 CPAALGPGFAAGTTDGPGAFGFQQGDTEVSTMESEMNPCLSFFDLSLNFVLEILLQINKL 518
           CPAALGPGFAAGTTDGPGAFGFQQGDT                            +INKL
Sbjct: 427 CPAALGPGFAAGTTDGPGAFGFQQGDT----------------------------KINKL 486

Query: 519 WKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGE 578
           W++LRD+LKKP+ +QV CQKPKTVLLDTGEMF PYAWAPAILPIQ+LRLGKLIILSVPGE
Sbjct: 487 WRKLRDALKKPTPYQVDCQKPKTVLLDTGEMFVPYAWAPAILPIQMLRLGKLIILSVPGE 546

Query: 579 FTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAST 638
           FTTMAGRRLREAVKETLISN NGEFDD+TH+VIAGLTNTYSQY+ATFEEY QQRYEAAST
Sbjct: 547 FTTMAGRRLREAVKETLISNSNGEFDDETHIVIAGLTNTYSQYIATFEEYAQQRYEAAST 606

Query: 639 LYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGEPPPNGVNFG 698
           LYGPHTLSAYIQEF+KLA A+AKGEKI K   SPPDLSSVQLRL+++P G+ PP   NFG
Sbjct: 607 LYGPHTLSAYIQEFEKLAKAMAKGEKITKG-PSPPDLSSVQLRLLLEPTGDLPPPHTNFG 666

Query: 699 DIQQDVKLPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDD 758
           D++QD+ LPK G FK G   +P ATFWSANPR+DLLTEGT+A+VE L+ + W P YDDDD
Sbjct: 667 DMKQDIILPKSGSFKKG--DRPIATFWSANPRYDLLTEGTFAVVELLQGKHWVPVYDDDD 726

Query: 759 FSLFFKWKLDNTFINSLVTIEWDIPVDTDPGVYRLRHFGSSRSTINSTNIYFTGASRGFA 799
           F LFFKWK+DN+ +    TIEW+IP D   GVYRLRHFGSS+ T +S NIYFTGAS GFA
Sbjct: 727 FCLFFKWKVDNSTLYGTATIEWEIPRDAVSGVYRLRHFGSSKKTKDSPNIYFTGASSGFA 756

BLAST of Chy3G056160 vs. NCBI nr
Match: XP_031742885.1 (neutral ceramidase 2 [Cucumis sativus])

HSP 1 Score: 1384 bits (3581), Expect = 0.0
Identity = 698/771 (90.53%), Postives = 706/771 (91.57%), Query Frame = 0

Query: 53  MGTVLVAVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVAT 112
           M TV+VAVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVA+
Sbjct: 1   MATVVVAVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVAS 60

Query: 113 SVDGPRIAFVNLDAGMASQLVTIK--------IFVQRFGDLYTEENVAISGIHTHAGPGG 172
           SVDGPRIAFVNLDAGMASQLVTIK        I VQRFGD+YTEENVAISGIHTHAGPGG
Sbjct: 61  SVDGPRIAFVNLDAGMASQLVTIKVLERLKSSILVQRFGDVYTEENVAISGIHTHAGPGG 120

Query: 173 YLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKLG----------------SPSA 232
           YLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLK G                SPSA
Sbjct: 121 YLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGEVENAGINRSPSA 180

Query: 233 YLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKG 292
           YLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKG
Sbjct: 181 YLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKG 240

Query: 293 AAARFFEDWAASNRRTTTTLSSNASGIEDLMKKAEKIKATGGKRCRKTSSKSSKVRKNDG 352
           AAARFFEDWAASNRRTTTT S+N SGIEDLMKKA KIKATGGKRC KTSSKSSKVRKNDG
Sbjct: 241 AAARFFEDWAASNRRTTTTPSNNTSGIEDLMKKAGKIKATGGKRCSKTSSKSSKVRKNDG 300

Query: 353 SLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTK 412
           SLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTK
Sbjct: 301 SLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTK 360

Query: 413 IIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALGPGFAA 472
           IIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEV IDGNNVVRTCPAALGPGFAA
Sbjct: 361 IIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVAIDGNNVVRTCPAALGPGFAA 420

Query: 473 GTTDGPGAFGFQQGDTEVSTMESEMNPCLSFFDLSLNFVLEILLQINKLWKQLRDSLKKP 532
           GTTDGPGAFGFQQGDTE                            INKLWKQLRDSLKKP
Sbjct: 421 GTTDGPGAFGFQQGDTE----------------------------INKLWKQLRDSLKKP 480

Query: 533 SEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLRE 592
           SEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLG LIILSVPGEFTTMAGRRLRE
Sbjct: 481 SEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGNLIILSVPGEFTTMAGRRLRE 540

Query: 593 AVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYI 652
           AVKETLISNGNG FDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYI
Sbjct: 541 AVKETLISNGNGVFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYI 600

Query: 653 QEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGEPPPNGVNFGDIQQDVKLPKD 712
           QEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGE PPNGVNFGDIQQDV+LPKD
Sbjct: 601 QEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGESPPNGVNFGDIQQDVELPKD 660

Query: 713 GWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSLFFKWKLDN 772
           GWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSLFFKWKLDN
Sbjct: 661 GWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSLFFKWKLDN 720

Query: 773 TFINSLVTIEWDIPVDTDPGVYRLRHFGSSRSTINSTNIYFTGASRGFAVF 799
           TFIN+LVTIEWDIP+D DPGVYRLRHFGSSRSTINSTN+YFTGASR FAVF
Sbjct: 721 TFINNLVTIEWDIPIDADPGVYRLRHFGSSRSTINSTNVYFTGASRAFAVF 743

BLAST of Chy3G056160 vs. NCBI nr
Match: XP_008464893.1 (PREDICTED: neutral ceramidase-like [Cucumis melo])

HSP 1 Score: 1345 bits (3481), Expect = 0.0
Identity = 683/776 (88.02%), Postives = 696/776 (89.69%), Query Frame = 0

Query: 53  MGTVLVAVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVAT 112
           M  V++ VSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQ TAGIHFRLRARTFVVA 
Sbjct: 1   MAIVVLDVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQVTAGIHFRLRARTFVVAR 60

Query: 113 SVDGPRIAFVNLDAGMASQLVTIKIF------------VQRFGDLYTEENVAISGIHTHA 172
           S+DGPRIAFVNLDAGMASQLVTIK+             VQRFGDLYTEENVAISGIHTHA
Sbjct: 61  SIDGPRIAFVNLDAGMASQLVTIKVLERLQSRRNINISVQRFGDLYTEENVAISGIHTHA 120

Query: 173 GPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKLG---------------- 232
           GPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLK G                
Sbjct: 121 GPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGEVDNAGINR 180

Query: 233 SPSAYLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISG 292
           SPSAYLMNPEEERAKYANNVDKEMSILKFVDGE+GNSIGAFSWFPTHGTSMSRDNKLISG
Sbjct: 181 SPSAYLMNPEEERAKYANNVDKEMSILKFVDGESGNSIGAFSWFPTHGTSMSRDNKLISG 240

Query: 293 DNKGAAARFFEDWAASNRRTTTTLSSNASGIEDLMKKAEKIKATGGKRCRKTSSKSSKVR 352
           DNKGAAARFFEDWAASN RTTTT S+NAS IE+LMKKAEKIKATGGK+C KTSSKSSKVR
Sbjct: 241 DNKGAAARFFEDWAASNGRTTTTPSNNASVIEELMKKAEKIKATGGKQCSKTSSKSSKVR 300

Query: 353 KNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEI 412
           KNDGSLFV AFCQSNVGDVTPNVLGAFC DSG PCDFN SSCHGSDLLCVGRGPGFPDEI
Sbjct: 301 KNDGSLFVAAFCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEI 360

Query: 413 LSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALGP 472
           LSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALGP
Sbjct: 361 LSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALGP 420

Query: 473 GFAAGTTDGPGAFGFQQGDTEVSTMESEMNPCLSFFDLSLNFVLEILLQINKLWKQLRDS 532
           GFAAGTTDGPG FGFQQGDTE                            INKLWKQLRDS
Sbjct: 421 GFAAGTTDGPGVFGFQQGDTE----------------------------INKLWKQLRDS 480

Query: 533 LKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGR 592
           LKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGR
Sbjct: 481 LKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGR 540

Query: 593 RLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTL 652
           RLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTL
Sbjct: 541 RLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTL 600

Query: 653 SAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGEPPPNGVNFGDIQQDVK 712
           SAYIQEFKKLA AIAKGEK+AKPIASPPDLSSVQLRLVMDPFGE PP+GVNFGDIQQDV+
Sbjct: 601 SAYIQEFKKLASAIAKGEKVAKPIASPPDLSSVQLRLVMDPFGESPPDGVNFGDIQQDVE 660

Query: 713 LPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSLFFKW 772
           LPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFS+ FKW
Sbjct: 661 LPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSVSFKW 720

Query: 773 KLDNT-FINSLVTIEWDIPVDTDPGVYRLRHFGSSRSTINSTNIYFTGASRGFAVF 799
           KLDNT  INSL TIEWDIP+D DPGVYRLRHFGSSRSTINSTNIYFTGASR FAVF
Sbjct: 721 KLDNTTLINSLATIEWDIPIDADPGVYRLRHFGSSRSTINSTNIYFTGASRAFAVF 748

BLAST of Chy3G056160 vs. NCBI nr
Match: XP_038885883.1 (neutral ceramidase 2-like [Benincasa hispida])

HSP 1 Score: 1305 bits (3378), Expect = 0.0
Identity = 655/767 (85.40%), Postives = 683/767 (89.05%), Query Frame = 0

Query: 53  MGTVLVAVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVAT 112
           M TV+ AVSWCVNGDWLVGVGSFDMTGPAA VNM+GYANMDQNTAGIHFRLRARTFVVA 
Sbjct: 1   MATVIAAVSWCVNGDWLVGVGSFDMTGPAAGVNMIGYANMDQNTAGIHFRLRARTFVVAK 60

Query: 113 SVDGPRIAFVNLDAGMASQLVTIKIFVQ---RFGDLYTEENVAISGIHTHAGPGGYLQYL 172
           +VDGPRIAF+NLDAGMASQLVTIK+  +   RFGDLYTEENVAISGIHTHAGPGGYLQYL
Sbjct: 61  TVDGPRIAFINLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYL 120

Query: 173 VYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKLG----------------SPSAYLMNP 232
           VYSITSLGFVQQSFDAIVNAIEQSIIQAHESLK G                SPSAYLMNP
Sbjct: 121 VYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGDVENGGINRSPSAYLMNP 180

Query: 233 EEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARF 292
           EEERA+YANNVDKEMS+LKFVDGE+GNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARF
Sbjct: 181 EEERARYANNVDKEMSVLKFVDGESGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARF 240

Query: 293 FEDWAASNRRTTTTLSSNASGIEDLMKKAEKIKATGGKRCRKTSSKSSKVRKNDGSLFVG 352
           FEDW AS +RT  T S+N SGI +LM+KAE IKATGGKRC KTSS++SKVRKNDGSLFV 
Sbjct: 241 FEDWVAS-KRTKITSSNNTSGIVELMRKAETIKATGGKRCSKTSSQASKVRKNDGSLFVA 300

Query: 353 AFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTKIIGER 412
           AFCQSNVGDVTPNVLGAFC DSG PCDFN SSCHGSDLLCVGRGPGFPDEILSTKIIGER
Sbjct: 301 AFCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGER 360

Query: 413 QFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALGPGFAAGTTDG 472
           QF KAADLFTTATEKLTGEIDFRHVYLNFTDIEVE+DGNNVV+TCPAALGPGFAAGTTDG
Sbjct: 361 QFQKAADLFTTATEKLTGEIDFRHVYLNFTDIEVELDGNNVVKTCPAALGPGFAAGTTDG 420

Query: 473 PGAFGFQQGDTEVSTMESEMNPCLSFFDLSLNFVLEILLQINKLWKQLRDSLKKPSEFQV 532
           PG FGFQQGDTE                            INKLWK+LRDSLKKPSEFQV
Sbjct: 421 PGVFGFQQGDTE----------------------------INKLWKKLRDSLKKPSEFQV 480

Query: 533 GCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKET 592
           GCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGR+LREAVKET
Sbjct: 481 GCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRQLREAVKET 540

Query: 593 LISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKK 652
           LI NGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKK
Sbjct: 541 LIRNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKK 600

Query: 653 LAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGEPPPNGVNFGDIQQDVKLPKDGWFKT 712
           LAIAIAK E IAKPIASPPDLSSVQLR V+DPFGE PPNG+NFGDIQQD+KLP +GWFK 
Sbjct: 601 LAIAIAKHENIAKPIASPPDLSSVQLRFVIDPFGESPPNGINFGDIQQDIKLPTNGWFKR 660

Query: 713 GSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSLFFKWKLDN-TFIN 772
           G KQKP ATFWSANPRFDLLTEGTYA+VERLEKQRWTP YDDDDFSLFFKW LDN TFIN
Sbjct: 661 GGKQKPAATFWSANPRFDLLTEGTYAVVERLEKQRWTPVYDDDDFSLFFKWTLDNNTFIN 720

Query: 773 SLVTIEWDIPVDTDPGVYRLRHFGSSRSTINSTNIYFTGASRGFAVF 799
           S+ TIEWDIP+D DPGVYRLRHFGSS+STINSTNIYFTGAS+ FAVF
Sbjct: 721 SIATIEWDIPIDADPGVYRLRHFGSSKSTINSTNIYFTGASQAFAVF 738

BLAST of Chy3G056160 vs. NCBI nr
Match: KAG7030054.1 (Neutral ceramidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1255 bits (3247), Expect = 0.0
Identity = 641/789 (81.24%), Postives = 673/789 (85.30%), Query Frame = 0

Query: 48  LKLMAMGTVLVAVSWC--VNGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRA 107
           LK++A   VL AVS C   +GDWLVGVGSFDMTGPAAEVNMMGYANMDQ+ AGIHFRLRA
Sbjct: 6   LKVVATA-VLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRA 65

Query: 108 RTFVVATSVDGPRIAFVNLDAGMASQLVTIKI------------------FVQRFGDLYT 167
           RTF+VA SVDGPRIAFVNLDAGMASQLVTIK+                  F+QRFG+LYT
Sbjct: 66  RTFIVAKSVDGPRIAFVNLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFGNLYT 125

Query: 168 EENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKLG--- 227
           EENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSI+QAH SLK G   
Sbjct: 126 EENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFIL 185

Query: 228 -------------SPSAYLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTH 287
                        SPSAYLMNP EERA+Y  NVD EMS++K V+G +G S+GAFSWFPTH
Sbjct: 186 INKGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTH 245

Query: 288 GTSMSRDNKLISGDNKGAAARFFEDWAASNRRTTTTLSSNASGIEDLMKKAEKIKATGGK 347
           GTSMSRDNKLISGDNKGAAARFFEDW A++ R+T   SSN S I +LMKKAE IKATGGK
Sbjct: 246 GTSMSRDNKLISGDNKGAAARFFEDWVAASNRSTRATSSNKSDIVELMKKAETIKATGGK 305

Query: 348 RCRKTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDL 407
           RC KTSS+SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSG PCDFN SSCHGSDL
Sbjct: 306 RCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDL 365

Query: 408 LCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDG 467
           LCVGRGPGFPDEILSTKIIGERQFLKA DLFTTATE+LTGEIDFRHVYLNFTDIEVE+ G
Sbjct: 366 LCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVELGG 425

Query: 468 NNVVRTCPAALGPGFAAGTTDGPGAFGFQQGDTEVSTMESEMNPCLSFFDLSLNFVLEIL 527
           +NVV+TCPAALGPGFAAGTTDGPG FGFQQGDT                           
Sbjct: 426 DNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDT--------------------------- 485

Query: 528 LQINKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLII 587
            QINKLWK+LRDSL+KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLII
Sbjct: 486 -QINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLII 545

Query: 588 LSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQR 647
           LSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTN YSQYVAT EEYEQQR
Sbjct: 546 LSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQR 605

Query: 648 YEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGEPPP 707
           YEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPI SPPDLSSVQLR V DPF E PP
Sbjct: 606 YEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPP 665

Query: 708 NGVNFGDIQQDVKLPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTP 767
           NGVNFGDIQQD+KLPK GWFK GSKQKPTA FWSANPRFDLLTEGTYA+VERLEK+RWTP
Sbjct: 666 NGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTP 725

Query: 768 AYDDDDFSLFFKWKLDN-TFINSLVTIEWDIPVDTDPGVYRLRHFGSSRSTINSTNIYFT 799
            YDDDDFSL FKW LDN TF NSL TIEWDIP+D +PGVYRLRHFGSS+S INSTNIYFT
Sbjct: 726 VYDDDDFSLVFKWALDNNTFTNSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFT 765

BLAST of Chy3G056160 vs. NCBI nr
Match: KAG6599114.1 (Neutral ceramidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1254 bits (3246), Expect = 0.0
Identity = 641/789 (81.24%), Postives = 673/789 (85.30%), Query Frame = 0

Query: 48  LKLMAMGTVLVAVSWC--VNGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRA 107
           LK++A   VL AVS C   +GDWLVGVGSFDMTGPAAEVNMMGYANMDQ+ AGIHFRLRA
Sbjct: 6   LKVVATA-VLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRA 65

Query: 108 RTFVVATSVDGPRIAFVNLDAGMASQLVTIKI------------------FVQRFGDLYT 167
           RTF+VA SVDGPRIAFVNLDAGMASQLVTIK+                  F+QRFG+LYT
Sbjct: 66  RTFIVAKSVDGPRIAFVNLDAGMASQLVTIKLLERLKASLTYLSKLKPRHFIQRFGNLYT 125

Query: 168 EENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKLG--- 227
           EENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSI+QAH SLK G   
Sbjct: 126 EENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFIL 185

Query: 228 -------------SPSAYLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTH 287
                        SPSAYLMNP EERA+Y  NVD EMS++K V+G +G S+GAFSWFPTH
Sbjct: 186 INKGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTH 245

Query: 288 GTSMSRDNKLISGDNKGAAARFFEDWAASNRRTTTTLSSNASGIEDLMKKAEKIKATGGK 347
           GTSMSRDNKLISGDNKGAAARFFEDW A++ R+T   SSN S I +LMKKAE IKATGGK
Sbjct: 246 GTSMSRDNKLISGDNKGAAARFFEDWVAASNRSTRATSSNKSDILELMKKAETIKATGGK 305

Query: 348 RCRKTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDL 407
           RC KTSS+SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSG PCDFN SSCHGSDL
Sbjct: 306 RCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDL 365

Query: 408 LCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDG 467
           LCVGRGPGFPDEILSTKIIGERQFLKA DLFTTATE+LTGEIDFRHVYLNFTDIEVE+ G
Sbjct: 366 LCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVELGG 425

Query: 468 NNVVRTCPAALGPGFAAGTTDGPGAFGFQQGDTEVSTMESEMNPCLSFFDLSLNFVLEIL 527
           +NVV+TCPAALGPGFAAGTTDGPG FGFQQGDT                           
Sbjct: 426 DNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDT--------------------------- 485

Query: 528 LQINKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLII 587
            QINKLWK+LRDSL+KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLII
Sbjct: 486 -QINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLII 545

Query: 588 LSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQR 647
           LSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTN YSQYVAT EEYEQQR
Sbjct: 546 LSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQR 605

Query: 648 YEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGEPPP 707
           YEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPI SPPDLSSVQLR V DPF E PP
Sbjct: 606 YEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPP 665

Query: 708 NGVNFGDIQQDVKLPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTP 767
           NGVNFGDIQQD+KLPK GWFK GSKQKPTA FWSANPRFDLLTEGTYA+VERLEK+RWTP
Sbjct: 666 NGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTP 725

Query: 768 AYDDDDFSLFFKWKLDN-TFINSLVTIEWDIPVDTDPGVYRLRHFGSSRSTINSTNIYFT 799
            YDDDDFSL FKW LDN TF NSL TIEWDIP+D +PGVYRLRHFGSS+S INSTNIYFT
Sbjct: 726 VYDDDDFSLVFKWALDNNTFTNSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFT 765

BLAST of Chy3G056160 vs. TAIR 10
Match: AT2G38010.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 781.9 bits (2018), Expect = 4.7e-226
Identity = 423/770 (54.94%), Postives = 526/770 (68.31%), Query Frame = 0

Query: 68  WLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVATSVDGPRIAFVNLDAG 127
           +L+GVGS+D+TGPAA+VNMMGYAN DQ  +GIHFRLRAR F+VA    G R+ FVNLDA 
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVA-EPQGNRVVFVNLDAC 85

Query: 128 MASQLVTIKIFVQ---RFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFD 187
           MASQ+VTIK+  +   R+G+LYTE+NVAISGIHTHAGPGGYLQY+ Y +TSLGFV+QSFD
Sbjct: 86  MASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFD 145

Query: 188 AIVNAIEQSIIQAHESLKLG----------------SPSAYLMNPEEERAKYANNVDKEM 247
            +VN IEQSI+QAHESL+ G                SPS+YL NP  ER+KY  +VDKEM
Sbjct: 146 VVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEM 205

Query: 248 SILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWAASN------- 307
           +++KFVD + G + G+F+WF THGTSMSR N LISGDNKGAAARF EDW  +        
Sbjct: 206 TLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSS 265

Query: 308 ----RRTTTTLSSNASGIEDLMKKAEKIKATGGKRCRKTSSKSSKVRKNDGSLFVGAFCQ 367
               RR +T +S  +   + L+  A   K++ G    K+    ++VR      FV AFCQ
Sbjct: 266 RNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQ 325

Query: 368 SNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLK 427
           SN GDV+PN LG FC D+G PCDFNHS+C+G + LC GRGPG+PDE  ST+IIGE+QF  
Sbjct: 326 SNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKM 385

Query: 428 AADLFTTATEKLTGEIDFRHVYLNFTDIEVEI----DGNNVVRTCPAALGPGFAAGTTDG 487
           A +LF  ATEKL G+I ++H YL+F++++V +     G+  V+TCPAA+G GFAAGTTDG
Sbjct: 386 AVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDG 445

Query: 488 PGAFGFQQGDTEVSTMESEMNPCLSFFDLSLNFVLEILLQINKLWKQLRDSLKKPSEFQV 547
           PGAF F+QGD                             Q N  W+ +R+ L+ P   QV
Sbjct: 446 PGAFDFKQGDD----------------------------QGNVFWRLVRNVLRTPGPEQV 505

Query: 548 GCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKET 607
            CQKPK +LLDTGEM EPY WAP+ILPIQILR+G+L+ILSVPGEFTTMAGRRLR+A+K  
Sbjct: 506 QCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSF 565

Query: 608 LISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKK 667
           LIS+   EF ++ HVVIAGLTNTYSQY+ATFEEYE QRYE ASTLYG HTL+AYIQEFKK
Sbjct: 566 LISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKK 625

Query: 668 LAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGEPPPNGVNFGDIQQDVKLPKDGWFKT 727
           LA A+  G  + +    PPDL   Q+ L+     +  P GV FGD++ DV  P    F+ 
Sbjct: 626 LATALVNGLTLPRG-PQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADV--PPKSTFRR 685

Query: 728 GSKQKPTATFWSANPRFDLLTEGTYALVERL-EKQRWTPAYDDDDFSLFFKW----KLDN 787
           G  Q+  ATFWS  PR DL+TEG++A+VE L E  +W P YDDDDFSL FKW    KL +
Sbjct: 686 G--QQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSS 745

Query: 788 TFINSLVTIEWDIPVDTDPGVYRLRHFGSSRSTINSTNIYFTGASRGFAV 799
               S  TIEW +P     GVYR+RH+G+S+S   S +  F+G+S  F V
Sbjct: 746 ---ESQATIEWRVPESAVAGVYRIRHYGASKSLFGSIS-SFSGSSSAFVV 756

BLAST of Chy3G056160 vs. TAIR 10
Match: AT2G38010.2 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 766.1 bits (1977), Expect = 2.7e-221
Identity = 424/805 (52.67%), Postives = 526/805 (65.34%), Query Frame = 0

Query: 68  WLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVATSVDGPRIAFVNLDAG 127
           +L+GVGS+D+TGPAA+VNMMGYAN DQ  +GIHFRLRAR F+VA    G R+ FVNLDA 
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVA-EPQGNRVVFVNLDAC 85

Query: 128 MASQLVTIKIFVQ---RFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFD 187
           MASQ+VTIK+  +   R+G+LYTE+NVAISGIHTHAGPGGYLQY+ Y +TSLGFV+QSFD
Sbjct: 86  MASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFD 145

Query: 188 AIVNAIEQSIIQAHESLKLG----------------SPSAYLMNPEEERAKYANNVDKEM 247
            +VN IEQSI+QAHESL+ G                SPS+YL NP  ER+KY  +VDKEM
Sbjct: 146 VVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEM 205

Query: 248 SILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWAASN------- 307
           +++KFVD + G + G+F+WF THGTSMSR N LISGDNKGAAARF EDW  +        
Sbjct: 206 TLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSS 265

Query: 308 ----RRTTTTLSSNASGIED-----------------------------------LMKKA 367
               RR +T +S  +   E                                    L+  A
Sbjct: 266 RNIPRRVSTIVSDFSRNRESYPFLELPNVAILYIAVAERLMWKVMVLFYFVSESRLLDIA 325

Query: 368 EKIKATGGKRCRKTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFN 427
              K++ G    K+    ++VR      FV AFCQSN GDV+PN LG FC D+G PCDFN
Sbjct: 326 ATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFN 385

Query: 428 HSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNF 487
           HS+C+G + LC GRGPG+PDE  ST+IIGE+QF  A +LF  ATEKL G+I ++H YL+F
Sbjct: 386 HSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDF 445

Query: 488 TDIEVEI----DGNNVVRTCPAALGPGFAAGTTDGPGAFGFQQGDTEVSTMESEMNPCLS 547
           ++++V +     G+  V+TCPAA+G GFAAGTTDGPGAF F+QGD               
Sbjct: 446 SNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDD-------------- 505

Query: 548 FFDLSLNFVLEILLQINKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAI 607
                         Q N  W+ +R+ L+ P   QV CQKPK +LLDTGEM EPY WAP+I
Sbjct: 506 --------------QGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSI 565

Query: 608 LPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYS 667
           LPIQILR+G+L+ILSVPGEFTTMAGRRLR+A+K  LIS+   EF ++ HVVIAGLTNTYS
Sbjct: 566 LPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYS 625

Query: 668 QYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQ 727
           QY+ATFEEYE QRYE ASTLYG HTL+AYIQEFKKLA A+  G  + +    PPDL   Q
Sbjct: 626 QYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPRG-PQPPDLLDKQ 685

Query: 728 LRLVMDPFGEPPPNGVNFGDIQQDVKLPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTY 787
           + L+     +  P GV FGD++ DV  P    F+ G  Q+  ATFWS  PR DL+TEG++
Sbjct: 686 ISLLSPVVVDSTPLGVKFGDVKADV--PPKSTFRRG--QQVNATFWSGCPRNDLMTEGSF 745

Query: 788 ALVERL-EKQRWTPAYDDDDFSLFFKW----KLDNTFINSLVTIEWDIPVDTDPGVYRLR 799
           A+VE L E  +W P YDDDDFSL FKW    KL +    S  TIEW +P     GVYR+R
Sbjct: 746 AVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSS---ESQATIEWRVPESAVAGVYRIR 791

BLAST of Chy3G056160 vs. TAIR 10
Match: AT1G07380.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 759.2 bits (1959), Expect = 3.3e-219
Identity = 411/780 (52.69%), Postives = 524/780 (67.18%), Query Frame = 0

Query: 65  NGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVATSVDGPRIAFVNL 124
           + ++L+G+GS+D+TGPAA+VNMMGYANM+Q  +GIHFRLRARTF+V +   G R+ FVNL
Sbjct: 34  HSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIV-SEPQGKRVVFVNL 93

Query: 125 DAGMASQLVTIKIFVQ---RFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQ 184
           DA MASQ+V +K+  +   R+GDLYTE+NV ISGIHTHAGPGGYLQY+VY +TSLGFV+Q
Sbjct: 94  DACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQ 153

Query: 185 SFDAIVNAIEQSIIQAHESLKLG----------------SPSAYLMNPEEERAKYANNVD 244
           SFDA+V+ IE SIIQAHE+L+ G                SPSAYL NP +ER+K+  NVD
Sbjct: 154 SFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNVD 213

Query: 245 KEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWAASN---- 304
           KEM++LKFVD + G  +G+F+WF THGTSMSR N LISGDNKGAA+RF EDW   N    
Sbjct: 214 KEMTLLKFVDDQWG-PVGSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAER 273

Query: 305 ------------RRTTTTLSSNASGIEDLMKKAEKIKATGGKRCRKTSSKSSKV----RK 364
                       RR ++ + ++     +L++ A   ++  GK   + SS + +V    RK
Sbjct: 274 SYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRISSSARRVRSALRK 333

Query: 365 NDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEIL 424
            D   FV AFCQ+N GDV+PNVLGAFC D+G PCDFNHS+C G + +C GRGPG+PDE  
Sbjct: 334 ADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEFE 393

Query: 425 STKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEI---DG-NNVVRTCPAA 484
           ST+IIGERQF  A +LF  A+E+L G++D+RHVY++F+ + V +   DG + VV+TCPAA
Sbjct: 394 STRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAA 453

Query: 485 LGPGFAAGTTDGPGAFGFQQGDTEVSTMESEMNPCLSFFDLSLNFVLEILLQINKLWKQL 544
           +G  FAAGTTDGPGAF F QGD +                             N  W+ +
Sbjct: 454 MGFAFAAGTTDGPGAFDFTQGDDKG----------------------------NPFWRLV 513

Query: 545 RDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTM 604
           R+ LK P + Q+ C  PK +LLDTGEM +PY WAP+IL +Q+LR+G+L ILSVPGEFTTM
Sbjct: 514 RNVLKTPDKKQIDCHYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTM 573

Query: 605 AGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGP 664
           AGRRLR AVK  L ++GN +   + HVVIAGL N YSQYV TFEEY+ QRYE ASTLYGP
Sbjct: 574 AGRRLRYAVKTQLKNSGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGP 633

Query: 665 HTLSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGEPPPNGVNFGDIQQ 724
           HTLS YIQEFKKL+ ++     + +P   PPDL   QL  +     +  P+G +FGD+  
Sbjct: 634 HTLSGYIQEFKKLSKSLVLDMPV-QPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVIS 693

Query: 725 DVKLPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEK--QRWTPAYDDDDFS 784
           DV  PK+   K G+ Q  T  F SA PR DLLTEGT+ LVERLE+  + WTP YDDDD  
Sbjct: 694 DV--PKNLSLKRGNGQ-VTVVFRSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLC 753

Query: 785 LFFKWKLDNTFIN-SLVTIEWDIPVDTDPGVYRLRHFGSSRSTINSTNIYFTGASRGFAV 799
           L FKW       + S  T+EW IP    PGVYR+ HFG+++    S + +FTG+S  F V
Sbjct: 754 LRFKWSRHKKLSSRSQATVEWRIPESASPGVYRITHFGAAKKLFGSVH-HFTGSSSAFVV 778

BLAST of Chy3G056160 vs. TAIR 10
Match: AT5G58980.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 722.6 bits (1864), Expect = 3.4e-208
Identity = 406/776 (52.32%), Postives = 505/776 (65.08%), Query Frame = 0

Query: 65  NGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVATSVDGPRIAFVNL 124
           + D+L+G+GS+D+TGPAA+VNMMGYANM+Q  +G+HFRLRAR F+VA      RIAFVNL
Sbjct: 26  DSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFIVAEPYK-KRIAFVNL 85

Query: 125 DAGMASQLVTIKI---FVQRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQ 184
           DAGMASQLVTIK+     QR+G+LYTEENVAISG HTHAGPGGYLQY++Y +TSLGFV Q
Sbjct: 86  DAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYLQYILYLVTSLGFVHQ 145

Query: 185 SFDAIVNAIEQSIIQAHESLKLG----------------SPSAYLMNPEEERAKYANNVD 244
           SF+A+V+ IEQSIIQAHE+L+ G                SPSAYL NP  ER+KY  +VD
Sbjct: 146 SFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYLNNPAHERSKYEYDVD 205

Query: 245 KEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDW-------- 304
           KEM+++KFVD                             D  G  AR  EDW        
Sbjct: 206 KEMTLVKFVD-----------------------------DQWGPVARIMEDWFERENGCR 265

Query: 305 ---AASNRRTTTTLSSNASGIEDLMKKAEKIKATGGKRCRKTSSKSSKVRKN----DGSL 364
                S RR ++ +S      +DLM+ A  + +TGGK   + SS + +VR      D   
Sbjct: 266 SVDVESPRRVSSIISDPYD--QDLMEMASSLLSTGGKTVTRMSSVARRVRSRFRHADKPR 325

Query: 365 FVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTKII 424
           FV AFCQ+N GDV+PNVLGAFC D+G PC+FN S+C G +  C GRGPG+PDE  ST+II
Sbjct: 326 FVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNEQCYGRGPGYPDEFESTRII 385

Query: 425 GERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNN----VVRTCPAALGPGF 484
           GERQF KAADLFT A+E++ G++D+RH Y++F+ +EV I+G N    VV+TCPAA+G GF
Sbjct: 386 GERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAMGFGF 445

Query: 485 AAGTTDGPGAFGFQQGDTEVSTMESEMNPCLSFFDLSLNFVLEILLQINKLWKQLRDSLK 544
           AAGTTDGPGAF F+QGD                             Q N  W+ +R+ LK
Sbjct: 446 AAGTTDGPGAFDFKQGDD----------------------------QGNPFWRLVRNLLK 505

Query: 545 KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRL 604
            P+E QV CQ+PK +LLDTGEM +PY WAP+ILP+QILR+G+L+IL VPGEFTTMAGRRL
Sbjct: 506 NPTEEQVRCQRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRL 565

Query: 605 REAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSA 664
           R+AVK  L    NG    +  VVIAGLTN+YSQY+ATFEEY+ QRYE ASTLYGPHTLS 
Sbjct: 566 RDAVKTVLKEGSNGR---EFSVVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSG 625

Query: 665 YIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGEPPPNGVNFGDIQQDVKLP 724
           YIQEFKKLA  +   +    P   PPDL   Q+ L+     +  P G  FGD+  DV  P
Sbjct: 626 YIQEFKKLANDLLSAQ-TTDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDV--P 685

Query: 725 KDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVER-LE-KQRWTPAYDDDDFSLFFKW 784
           +   F+ G+       F SANPR DL+TEGT+ALVER LE ++ W P YDDDDF L FKW
Sbjct: 686 RLSKFRKGA-DIVRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKW 733

Query: 785 KLD-NTFINSLVTIEWDIPVDTDPGVYRLRHFGSSRSTINSTNIYFTGASRGFAVF 800
                    S  TIEW IP    PGVYR+ HFGS+++ I+S + +F+G+S  F V+
Sbjct: 746 SRPFKLSTQSTATIEWRIPETASPGVYRITHFGSAKTPISSIH-HFSGSSSAFVVY 733