Chy12G218280 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G218280
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	cytochrome P450 71A1-like
Location	chrH12: 17006735 .. 17008592 (+)
RNA-Seq Expression	Chy12G218280
Synteny	Chy12G218280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGAACAAGTCATGAAAACCCATGACCTTGTATTCTCTAACCGACCCCAAACGACTGCGGCAAAAACGTTGGTTTACGGATGCCAAGATGTGGGTTTTGCCCCATATGGCGAGTATTGGCGCCAAGCGAAAAAAATATGTACTCTTGAGCTTTTTACTGTGAAAAGAGTTGACTCTTTTCAATACGTTAGAAATGAAGAAATTGATGCTCTTGTTAATAAGATTCTTAAGAGTGGGAGTGATCAAAGTTTGGATCTTGGCCATTTGTTTTTCCAAACATCAAATAATATTGTTTCAAGATGTGTTCTGGGAGAGAAATTTGAGGATGAAGATGGGAAGAGTAGATTTGAGGAGATATCAAGAAAGGCTATGGTGATAGTAACAGCGTTTTGTGTTGAGGATTTTTTCCCTTCATTTGGTTGGATTATTGATGTTATTAGAGGATTTAATTGGGATTTGAAGAATTGTTTCAAAACATTAGATGAGTTTTTCAGTAAAGTTGTTGAAGAACATAAGGAGAAGATTAAGAGATCAGGAGGTGTAATTAATATCGATGATTATGAATCAAAAAAGGATTTTGTGGATATTATGCTACAACTTCAACAAGATGACAAGCTTGACTATCATTTCTCTCTTGACAACCTCAAAGCAATTGTTTTGGTTAGTTCTCTCTTTCTTAATTCTCTTTATATATTACACTACATCCATTTTTTAGGATACAATTGACACAAGGTAAAAAAAGAAAAGAGAGAATTTGATAAAGAAAAATCAAAATTTAATTCTCACAATCTAAAGAGTGGTTAGACTTTAATTGTCAAACTGTATAAATATTACTTTATTGACTTTGTTAGTTTGATAAACAATTCTTCTAAACACTCTACCATGTAATTATATATTATATAAACTAAATTCTAATTTTTCTCACAGCTCATGAGAATTAGATTTACAATTTAAGTTAATTAATTAATATTCTCCCCCCATTTTAAATAAATGTCATATTGTCATTACTTTTCTCCTAAAACTTCTCACCAGCATTTGTGCTTTTAATTTTGACCTTCAACAGGAAAACAACAAAGCAGTTTTTCCTGACGATGTGTCTGTCCTCTTTGTTTTTTGTTTTTAATATGTAAAAAAATAAGAACACTCAATATTGAGAGGTATTTTTAGAAATCTTTTGAAAATACAAATTTTTATAGAAAAAAAAATATTTGATTAGGTTTGTATTTTGCATGTATAGGACATGTTTGTAGGTGGAAGTGACACAACAGCAACAGGATTGGAATGGACAATGACAGAGCTAATGAGAAAACCAAGAATCATGAAGAAAGTTCAAGAAGAAGTTAGAACAATAATTGGCAAAAAATCAAAGATAGAAGCAGAAGACATTCAAAAAATGGAATACATGCAATGTGTCATTAAAGAGTCTCTAAGGCTCCACCCACCCGTTCCTCTTTTATTGCCAAGAGAAACAATGGCAGACGTTGAGATTGAAGGTTACTATATTCCATCAAAAACAAGAGTGTTTGTGAATGCTTGGGCCATTCAAAGAGACCCTCAATGTTGGAATAATCCAAATGAATTCATTCCAGAGAGATTTATGGACAAAACTAATTCAACTGATTATAAAGGTCAAAATTTTGAGTTCATTCCATTTGGTAGTGGAAGAAGGAAGTGTCCTGGATTGTCATTTGGGATTGCTTCTTTTGAATTTGCTTTGGCTAATATTCTCTATTGGTTTGATTGGAAGCTTCCTGGTGGTTGTGAATCATTGGATGTTGAAGAAACTAATGGACTCACTGTTCGTAAGAAAAAGTCACTCCATCTTAACCCCATACCTTATGTTGTGTCTAATTAA

mRNA sequence

ATGGCCGAACAAGTCATGAAAACCCATGACCTTGTATTCTCTAACCGACCCCAAACGACTGCGGCAAAAACGTTGGTTTACGGATGCCAAGATGTGGGTTTTGCCCCATATGGCGAGTATTGGCGCCAAGCGAAAAAAATATGTACTCTTGAGCTTTTTACTGTGAAAAGAGTTGACTCTTTTCAATACGTTAGAAATGAAGAAATTGATGCTCTTGTTAATAAGATTCTTAAGAGTGGGAGTGATCAAAGTTTGGATCTTGGCCATTTGTTTTTCCAAACATCAAATAATATTGTTTCAAGATGTGTTCTGGGAGAGAAATTTGAGGATGAAGATGGGAAGAGTAGATTTGAGGAGATATCAAGAAAGGCTATGGTGATAGTAACAGCGTTTTGTGTTGAGGATTTTTTCCCTTCATTTGGTTGGATTATTGATGTTATTAGAGGATTTAATTGGGATTTGAAGAATTGTTTCAAAACATTAGATGAGTTTTTCAGTAAAGTTGTTGAAGAACATAAGGAGAAGATTAAGAGATCAGGAGGTGTAATTAATATCGATGATTATGAATCAAAAAAGGATTTTGTGGATATTATGCTACAACTTCAACAAGATGACAAGCTTGACTATCATTTCTCTCTTGACAACCTCAAAGCAATTGTTTTGGACATGTTTGTAGGTGGAAGTGACACAACAGCAACAGGATTGGAATGGACAATGACAGAGCTAATGAGAAAACCAAGAATCATGAAGAAAGTTCAAGAAGAAGTTAGAACAATAATTGGCAAAAAATCAAAGATAGAAGCAGAAGACATTCAAAAAATGGAATACATGCAATGTGTCATTAAAGAGTCTCTAAGGCTCCACCCACCCGTTCCTCTTTTATTGCCAAGAGAAACAATGGCAGACGTTGAGATTGAAGGTTACTATATTCCATCAAAAACAAGAGTGTTTGTGAATGCTTGGGCCATTCAAAGAGACCCTCAATGTTGGAATAATCCAAATGAATTCATTCCAGAGAGATTTATGGACAAAACTAATTCAACTGATTATAAAGGTCAAAATTTTGAGTTCATTCCATTTGGTAGTGGAAGAAGGAAGTGTCCTGGATTGTCATTTGGGATTGCTTCTTTTGAATTTGCTTTGGCTAATATTCTCTATTGGTTTGATTGGAAGCTTCCTGGTGGTTGTGAATCATTGGATGTTGAAGAAACTAATGGACTCACTGTTCGTAAGAAAAAGTCACTCCATCTTAACCCCATACCTTATGTTGTGTCTAATTAA

Coding sequence (CDS)

ATGGCCGAACAAGTCATGAAAACCCATGACCTTGTATTCTCTAACCGACCCCAAACGACTGCGGCAAAAACGTTGGTTTACGGATGCCAAGATGTGGGTTTTGCCCCATATGGCGAGTATTGGCGCCAAGCGAAAAAAATATGTACTCTTGAGCTTTTTACTGTGAAAAGAGTTGACTCTTTTCAATACGTTAGAAATGAAGAAATTGATGCTCTTGTTAATAAGATTCTTAAGAGTGGGAGTGATCAAAGTTTGGATCTTGGCCATTTGTTTTTCCAAACATCAAATAATATTGTTTCAAGATGTGTTCTGGGAGAGAAATTTGAGGATGAAGATGGGAAGAGTAGATTTGAGGAGATATCAAGAAAGGCTATGGTGATAGTAACAGCGTTTTGTGTTGAGGATTTTTTCCCTTCATTTGGTTGGATTATTGATGTTATTAGAGGATTTAATTGGGATTTGAAGAATTGTTTCAAAACATTAGATGAGTTTTTCAGTAAAGTTGTTGAAGAACATAAGGAGAAGATTAAGAGATCAGGAGGTGTAATTAATATCGATGATTATGAATCAAAAAAGGATTTTGTGGATATTATGCTACAACTTCAACAAGATGACAAGCTTGACTATCATTTCTCTCTTGACAACCTCAAAGCAATTGTTTTGGACATGTTTGTAGGTGGAAGTGACACAACAGCAACAGGATTGGAATGGACAATGACAGAGCTAATGAGAAAACCAAGAATCATGAAGAAAGTTCAAGAAGAAGTTAGAACAATAATTGGCAAAAAATCAAAGATAGAAGCAGAAGACATTCAAAAAATGGAATACATGCAATGTGTCATTAAAGAGTCTCTAAGGCTCCACCCACCCGTTCCTCTTTTATTGCCAAGAGAAACAATGGCAGACGTTGAGATTGAAGGTTACTATATTCCATCAAAAACAAGAGTGTTTGTGAATGCTTGGGCCATTCAAAGAGACCCTCAATGTTGGAATAATCCAAATGAATTCATTCCAGAGAGATTTATGGACAAAACTAATTCAACTGATTATAAAGGTCAAAATTTTGAGTTCATTCCATTTGGTAGTGGAAGAAGGAAGTGTCCTGGATTGTCATTTGGGATTGCTTCTTTTGAATTTGCTTTGGCTAATATTCTCTATTGGTTTGATTGGAAGCTTCCTGGTGGTTGTGAATCATTGGATGTTGAAGAAACTAATGGACTCACTGTTCGTAAGAAAAAGTCACTCCATCTTAACCCCATACCTTATGTTGTGTCTAATTAA

Protein sequence

MAEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDSFQYVRNEEIDALVNKILKSGSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSRFEEISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPGGCESLDVEETNGLTVRKKKSLHLNPIPYVVSN*

Homology

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BLAST of Chy12G218280 vs. ExPASy Swiss-Prot
Match: A0A068Q609 (Phenylacetaldehyde oxime monooxygenase CYP71AN24 OS=Prunus mume OX=102107 GN=CYP71AN24 PE=1 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 4.1e-128
Identity = 228/429 (53.15%), Postives = 301/429 (70.16%), Query Frame = 0


Query: 1   MAEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDS 60
           MA+ VMKT D+VF +RPQTTA   L Y   D+ FAPYGEYWRQ ++IC LEL ++KRV  
Sbjct: 108 MAKDVMKTQDIVFCSRPQTTAPSILFYDGHDIAFAPYGEYWRQVRRICVLELLSLKRVHQ 167

Query: 61  FQYVRNEEIDALVNKILK---SGSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSRF 120
           FQY R EE+  LV+KI K   S +   ++LG L   TSNNI+ RC+LG+KFED++  + F
Sbjct: 168 FQYARVEEVAELVSKIRKASASANGAPINLGELLVSTSNNIICRCILGQKFEDKE-DNWF 227

Query: 121 EEISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKIK 180
            E +++ M  V +F   DFFPS  W ID  RG+   LK+ +   D+FF K+++EHK   K
Sbjct: 228 GETTKELMTQVMSFSFGDFFPSLKW-IDRARGYLAYLKSIWLEFDKFFDKLIDEHKAAQK 287

Query: 181 RSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGLEW 240
                   +    KKD VDI+L +Q D  LD+  +  N+KAI+ DMFVGGSDT+ T   W
Sbjct: 288 --------EGKPRKKDIVDILLDVQNDGSLDFELTTSNVKAILQDMFVGGSDTSWTAAIW 347

Query: 241 TMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPR 300
            M+EL + PR+MKKVQEEVR + GK+  +E  DI +M+Y+ CVIKE+LRLHPP PLLLPR
Sbjct: 348 LMSELSQNPRVMKKVQEEVRRVAGKRGYVEESDINEMKYLTCVIKENLRLHPPAPLLLPR 407

Query: 301 ETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQNFEF 360
           E M+DV++ G+ IP+KT+VFVNA+A+QRDP+ W+ P+EF+PERF  + N+  + GQ+FE 
Sbjct: 408 EAMSDVKLGGFDIPAKTQVFVNAYAVQRDPKVWDKPDEFMPERF--EENNVGFVGQDFEL 467

Query: 361 IPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPGG----CESLDVEETNGLTVRKKK 420
           IPFG+GRR CPGL+FG+AS ++ LAN+LYWFDWKLP G     E+LD+ E  GLTV KK 
Sbjct: 468 IPFGAGRRVCPGLAFGVASAQYVLANMLYWFDWKLPSGGSKLAETLDMSEVYGLTVHKKS 524

Query: 421 SLHLNPIPY 423
            L+L P PY
Sbjct: 528 PLYLVPTPY 524

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BLAST of Chy12G218280 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 438.3 bits (1126), Expect = 9.7e-122
Identity = 204/421 (48.46%), Postives = 301/421 (71.50%), Query Frame = 0


Query: 1   MAEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDS 60
           +AE+++KTHDL+F++RP TTAA+ + Y C DV F+PYGEYWRQ +KIC LEL ++KRV+S
Sbjct: 83  IAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVRKICVLELLSIKRVNS 142

Query: 61  FQYVRNEEIDALVNKILKS-GSDQSLDLGHLFFQTSNNIVSRCVLGEKFE-DEDGKSRFE 120
           ++ +R EE+  ++ +I +S  + ++++L  L    S+  ++R   G+K+E +E+ K++F 
Sbjct: 143 YRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITRVAFGKKYEGEEERKNKFA 202

Query: 121 EISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKIKR 180
           +++ +   ++ AF V D+FPSF W +DV+ G +  LK     LD F   V+++H    K 
Sbjct: 203 DLATELTTLMGAFFVGDYFPSFAW-VDVLTGMDARLKRNHGELDAFVDHVIDDHLLSRKA 262

Query: 181 SGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGLEWT 240
           +G      D   +KD VD++L LQ+D  L  H + +NLKA++LDMF GG+DTTA  LEW 
Sbjct: 263 NG-----SDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMFSGGTDTTAVTLEWA 322

Query: 241 MTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPRE 300
           M EL++ P +M+K Q+EVR ++GKK+K+E ED+ ++ Y++ +IKE+LRLHP  PLL+PRE
Sbjct: 323 MAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKETLRLHPVAPLLVPRE 382

Query: 301 TMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQNFEFI 360
           +  DV I GY+IP+KTRVF+NAWAI RDP+ W N  EF+PERF++  NS D+KGQ+F+ I
Sbjct: 383 STRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFVN--NSVDFKGQDFQLI 442

Query: 361 PFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPGGC--ESLDVEETNGLTVRKKKSLH 418
           PFG+GRR CPG++FGI+S E +LAN+LYWF+W+LPG    E LD+ E  G+TV  K  L 
Sbjct: 443 PFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLDMSEAVGITVHMKFPLQ 495

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BLAST of Chy12G218280 vs. ExPASy Swiss-Prot
Match: A0A0N9HTU1 (Desmethylyatein synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71CU1 PE=1 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 1.1e-106
Identity = 186/422 (44.08%), Postives = 271/422 (64.22%), Query Frame = 0


Query: 2   AEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDSF 61
           A+++MKTHDL  +NRP TTAA+ L+Y C D+ FAPYGEYWR+ KK+  L L ++K++ SF
Sbjct: 83  AKEIMKTHDLALANRPLTTAARALLYDCTDISFAPYGEYWREMKKMAVLNLLSIKKIQSF 142

Query: 62  QYVRNEEIDALVNKILK-SGSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSRFEEI 121
           + VR E    ++ +I + S +   +D+ ++ +  S +++ RC LG K     G+ +F+++
Sbjct: 143 RSVREELASDMIKEITRLSKTGAPVDVTNMLYHFSEDLLFRCTLGFK---PKGQHKFQKL 202

Query: 122 SRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKIKRSG 181
           SR  + +V AFC  DFFP   W +D + G N  LK   + LD+F  +++EE    +K   
Sbjct: 203 SRDFLDLVGAFCFNDFFPGMAW-MDALTGLNRKLKKGSRELDDFVDELIEERIAMVK--- 262

Query: 182 GVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGLEWTMT 241
                 D     +F+D++L   +D   +   + DN+KAI+LD F+GG D  A+ +EW M 
Sbjct: 263 ------DGVEPNEFLDLLLHTHRDTTQEIKLTRDNVKAIILDTFLGGIDLPASVMEWAMA 322

Query: 242 ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPRETM 301
           ELMR P  MK  QEEVR ++G K+K++ +D+ +M +++  +KE+LRLHPP PLL  RE+ 
Sbjct: 323 ELMRNPSKMKIAQEEVRKVVGNKNKVDEDDVYQMNFLKSAVKETLRLHPPAPLLFARESY 382

Query: 302 ADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQNFEFIPF 361
             + +E Y IP  T V +N W IQRDP+ W+   EFIPERFM+  +  DYK  ++EFIPF
Sbjct: 383 TSINVENYIIPPYTSVMINIWHIQRDPKLWDKAEEFIPERFMN--SGIDYKSHDYEFIPF 442

Query: 362 GSGRRKCPGLSFGIASFEFALANILYWFDWKLPGGC--ESLDVEETNGLTVRKKKSLHLN 421
           GSGRR CPG+SFG+A+ EFA+AN+LYWFDWK  G    E+LD+ E     + KKK LH  
Sbjct: 443 GSGRRGCPGMSFGVAAVEFAVANLLYWFDWKFVGDTTPETLDMTEDYCFALFKKKPLHFI 489

[Close]

BLAST of Chy12G218280 vs. ExPASy Swiss-Prot
Match: Q9LTM0 (Cytochrome P450 71B23 OS=Arabidopsis thaliana OX=3702 GN=CYP71B23 PE=2 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.3e-102
Identity = 194/429 (45.22%), Postives = 278/429 (64.80%), Query Frame = 0


Query: 2   AEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDSF 61
           AE+V+KTHDL   +RP+T A+KT+ Y  +D+GFAPYGE WR  +K+  +ELF++K+ +SF
Sbjct: 84  AEEVLKTHDLDCCSRPETIASKTISYNFKDIGFAPYGEEWRALRKLAVIELFSLKKFNSF 143

Query: 62  QYVRNEEIDALVNKILKSGSDQS-LDLGHLFFQTSNNIVSRCVLGE-----KFEDEDGKS 121
           +Y+R EE D LV K+ ++   QS ++L    F  S +IV R   G+     +F DED   
Sbjct: 144 RYIREEENDLLVKKLSEASEKQSPVNLKKALFTLSASIVCRLAFGQNLHESEFIDEDS-- 203

Query: 122 RFEEISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEK 181
             E+++ ++  I   F   +FFP  GWI+D I G +  L   F  LD FF++V+++H   
Sbjct: 204 -MEDLASRSEKIQAKFAFSNFFPG-GWILDKITGQSKSLNEIFADLDGFFNQVLDDH--- 263

Query: 182 IKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSL--DNLKAIVLDMFVGGSDTTAT 241
           +K    V+         D VD+M+ +      D  F L  D++K I+ D+F+ G +T+AT
Sbjct: 264 LKPGRKVL------ETPDVVDVMIDMMNKQSQDGSFKLTTDHIKGIISDIFLAGVNTSAT 323

Query: 242 GLEWTMTELMRKPRIMKKVQEEVRTIIG-KKSKIEAEDIQKMEYMQCVIKESLRLHPPVP 301
            + W MTEL+R PR+MKKVQ+EVRT++G K+ +I  +D+ ++ Y + VIKE+ RLHP  P
Sbjct: 324 TILWAMTELIRNPRVMKKVQDEVRTVLGEKRDRITEQDLNQLNYFKLVIKETFRLHPAAP 383

Query: 302 LLLPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKG 361
           LLLPRE MA ++I+GY IP KT++ VN +AI RDP  W NP EF PERF+D  +S DY+G
Sbjct: 384 LLLPREAMAKIKIQGYDIPEKTQIMVNVYAIGRDPDLWENPEEFKPERFVD--SSVDYRG 443

Query: 362 QNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPGG--CESLDVEETNGLTVR 420
            NFE +PFGSGRR CPG++ GIA+ E  L N+LY+FDW LP G   + +D+EE   + + 
Sbjct: 444 LNFELLPFGSGRRICPGMTMGIATVELGLLNLLYFFDWGLPEGRTVKDIDLEEEGAIIIG 497

[Close]

BLAST of Chy12G218280 vs. ExPASy Swiss-Prot
Match: A0A0N9HT29 (Desmethyl-deoxy-podophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71BE54 PE=1 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 3.8e-102
Identity = 189/421 (44.89%), Postives = 269/421 (63.90%), Query Frame = 0


Query: 1   MAEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDS 60
           MA  +M THDL+F+NR    A   L+YG + +G APYG+YWRQ +KIC LEL + KRV S
Sbjct: 86  MARAIMSTHDLMFANRTVQFATDILLYGGKGIGLAPYGDYWRQIRKICVLELLSAKRVQS 145

Query: 61  FQYVRNEEIDALVNKILKSGSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSRFEEI 120
           FQ VR EEI  L+  I  S     ++   +    +N+I++R   GEK +D   K  F  +
Sbjct: 146 FQTVREEEISNLIRSI--SAESSKVNFSEMITSLTNDIIARAAFGEKCKD---KHAFLSL 205

Query: 121 SRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKIKRSG 180
             + + +   F +   FPS   ++ VI G    L+     LD     +++E+KE  KR  
Sbjct: 206 IEEGIQLAGGFDIAGLFPSLK-LLHVITGKKRQLERVHNELDRILVDIIKENKE--KRRA 265

Query: 181 GVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGLEWTMT 240
             +N D YE  ++ VD+++++Q++ +L+   + DNLKA++LD+FV GS+T++T +EW M+
Sbjct: 266 SKLNEDKYE--ENLVDVLVRVQENTQLEVPLANDNLKAVILDIFVAGSETSSTTIEWAMS 325

Query: 241 ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPRETM 300
           E+++ P++MKK Q EVR +     KI   +IQ++ Y++ V+KE+LRLH P PLL+PRE  
Sbjct: 326 EMLKNPKVMKKAQAEVRRVFSGNKKINETEIQELSYLKLVLKETLRLHAPAPLLIPRECR 385

Query: 301 ADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQNFEFIPF 360
              EI+GY IP KT V VNAWAI RDP+ W+N + F PERF     S DYKG NFE+IPF
Sbjct: 386 ESCEIDGYEIPKKTMVMVNAWAIGRDPENWSNGDRFEPERF--DGISVDYKGTNFEYIPF 445

Query: 361 GSGRRKCPGLSFGIASFEFALANILYWFDWKLPGGC--ESLDVEETNGLTVRKKKSLHLN 420
           G+GRR CPG+ FG+A+ E  LA +LY FDW+LP G   E LD++ET G TVR+K  L+L 
Sbjct: 446 GAGRRICPGMLFGMANVEVPLARLLYHFDWELPNGIKPEELDMDETFGTTVRRKNHLYLI 494

[Close]

BLAST of Chy12G218280 vs. ExPASy TrEMBL
Match: A0A6J1C631 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008616 PE=3 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 1.9e-176
Identity = 309/428 (72.20%), Postives = 352/428 (82.24%), Query Frame = 0


Query: 1   MAEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDS 60
           MA +VMKTHDL FS+RPQTTAAK L+YG +DVGFAPYGEYWRQA+KIC LELF+ KRV+S
Sbjct: 95  MAREVMKTHDLNFSDRPQTTAAKILLYGFKDVGFAPYGEYWRQARKICALELFSAKRVES 154

Query: 61  FQYVRNEEIDALVNKILK----SGSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSR 120
           FQYVR+EE+  LVN+I +       + S++L  LF  TSNNIVSRC++G+KFEDE+GKSR
Sbjct: 155 FQYVRDEEVAVLVNRIRRGCVGGDGEGSVNLAQLFLSTSNNIVSRCMMGDKFEDENGKSR 214

Query: 121 FEEISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKI 180
           F +ISR+ MV++TAFC  DFFP+  W IDV+RGFN +LK CF TLD FFSKVVEEHK K+
Sbjct: 215 FGDISRRVMVLLTAFCFADFFPALWW-IDVVRGFNGELKKCFTTLDAFFSKVVEEHKAKM 274

Query: 181 KRSGGVINIDDYESKKDFVDIMLQL-QQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGL 240
              G + +    ESK+DF+D MLQL  QDD +DY FSLDNLKAIVLDMFVGGSDTTATGL
Sbjct: 275 IGGGELCD----ESKRDFMDSMLQLILQDDMVDYRFSLDNLKAIVLDMFVGGSDTTATGL 334

Query: 241 EWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLL 300
           EWTMTELMR P +MKKVQEEVR I+G K KIE EDIQ M YM CVIKESLRLHPPVPLL+
Sbjct: 335 EWTMTELMRNPSVMKKVQEEVRKIVGNKEKIEMEDIQNMPYMNCVIKESLRLHPPVPLLV 394

Query: 301 PRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQNF 360
           PRE + DVEIEGY+IPSKTRVFVNAW I RDP  W  PNEFIPERFM+  NS DYKGQ+F
Sbjct: 395 PREAIGDVEIEGYHIPSKTRVFVNAWKIHRDPNIWERPNEFIPERFME--NSIDYKGQDF 454

Query: 361 EFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPGGCESLDVEETNGLTVRKKKSL 420
           +FIPFGSGRR CPG SFGIASFE+ALAN+LYWFDWKLP G + LDVEE NGLTVRKK  L
Sbjct: 455 DFIPFGSGRRICPGKSFGIASFEYALANLLYWFDWKLPHGSKELDVEEENGLTVRKKIPL 514

Query: 421 HLNPIPYV 424
           HLNPI Y+
Sbjct: 515 HLNPISYI 515

[Close]

BLAST of Chy12G218280 vs. ExPASy TrEMBL
Match: A0A6J1C5L5 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008617 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 5.4e-176
Identity = 309/424 (72.88%), Postives = 351/424 (82.78%), Query Frame = 0


Query: 1   MAEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDS 60
           MA ++MKTHDL FSNRPQT  AKTL+YG +DVGFAPYGEYWRQA+KIC LELF+ KRV+S
Sbjct: 97  MARELMKTHDLKFSNRPQTMPAKTLLYGFKDVGFAPYGEYWRQARKICALELFSAKRVES 156

Query: 61  FQYVRNEEIDALVNKILK----SGSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSR 120
           FQYVR+EE+  LVN+I K       + S++L  LF  TSNNIVSRC+LGEKFEDE+GKSR
Sbjct: 157 FQYVRDEEVSVLVNRIRKGCVDGDGEGSVNLSQLFLSTSNNIVSRCILGEKFEDENGKSR 216

Query: 121 FEEISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKI 180
           F + SR+ MV++TAFC  DFFP+  W IDV+RGFN +LK  F TLD F SKVVEEHK K+
Sbjct: 217 FGDTSRRVMVLITAFCFADFFPALWW-IDVVRGFNGELKKWFTTLDAFLSKVVEEHKAKM 276

Query: 181 KRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGLE 240
            R+GG    +  ESKKDFV IMLQL Q D +DYHFSL+NLK IVLDMFVGG+DTTATGLE
Sbjct: 277 -RAGG----ESDESKKDFVGIMLQLMQSDMVDYHFSLENLKGIVLDMFVGGTDTTATGLE 336

Query: 241 WTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLP 300
           WTMTELMR P +M+KVQEEVR  +GKK+KIE EDI+KM YM CVIKESLRLHPPVPLL+P
Sbjct: 337 WTMTELMRNPPVMEKVQEEVRKTVGKKAKIEIEDIRKMHYMNCVIKESLRLHPPVPLLVP 396

Query: 301 RETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQNFE 360
           RE ++DVEIEGY+IPSKTRVFVNAW I RDP  W  PNEFIPERFM+  NS DYKGQ+FE
Sbjct: 397 REAISDVEIEGYHIPSKTRVFVNAWKIHRDPTIWERPNEFIPERFME--NSVDYKGQDFE 456

Query: 361 FIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPGGCESLDVEETNGLTVRKKKSLH 420
           FIPFGSGRR CPG+SFGIASFE+ALAN+LYWFDWKLP G E LDVEE NGLTVRKK  LH
Sbjct: 457 FIPFGSGRRICPGMSFGIASFEYALANLLYWFDWKLPNGFE-LDVEEENGLTVRKKNPLH 511

[Close]

BLAST of Chy12G218280 vs. ExPASy TrEMBL
Match: A0A6J1GRT5 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456925 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 7.8e-175
Identity = 310/422 (73.46%), Postives = 349/422 (82.70%), Query Frame = 0


Query: 1   MAEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDS 60
           MA +VMKTHD+ FSNRPQTTAA  L+Y CQD+GFA YGEYWRQA+K+C LELF+VKRVDS
Sbjct: 93  MAREVMKTHDIKFSNRPQTTAAHELLYQCQDIGFAQYGEYWRQARKVCALELFSVKRVDS 152

Query: 61  FQYVRNEEIDALVNKILKS-GSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSRFEE 120
           FQYVR+EE+  L+++I K+    ++++L  LF QTSNNIVSRCVLGEKFEDE+G SRF  
Sbjct: 153 FQYVRDEEVGGLMDRIRKACVGGEAVNLSQLFLQTSNNIVSRCVLGEKFEDENGNSRFGV 212

Query: 121 ISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKIKRS 180
             R+AMV++ AFCV D FPS  W ID+IRGFN +LK+C KTLD F SKV+EEHKEKI+  
Sbjct: 213 TVRRAMVLIVAFCVADLFPSLWW-IDIIRGFNKELKDCSKTLDTFVSKVIEEHKEKIRDG 272

Query: 181 GGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGLEWTM 240
            G       ESKKDFVDIMLQLQQDD +DYHFS D+LKA++LDMFVGGSDTTAT LEWTM
Sbjct: 273 VGT-----DESKKDFVDIMLQLQQDDMVDYHFSPDSLKAMLLDMFVGGSDTTATTLEWTM 332

Query: 241 TELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPRET 300
           TELMR P  MKKVQEEVRTI GKKSKIE EDIQKMEYM CVI ESLRLHPPVPLL+PRET
Sbjct: 333 TELMRNPSTMKKVQEEVRTITGKKSKIEVEDIQKMEYMHCVINESLRLHPPVPLLVPRET 392

Query: 301 MADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQNFEFIP 360
            ADVEI+GY+IPSKTRVFVNAW IQRD + W NP EFIPERFMD   S DYKGQ +EFIP
Sbjct: 393 AADVEIDGYHIPSKTRVFVNAWKIQRDLEYWKNPTEFIPERFMD-NRSVDYKGQCYEFIP 452

Query: 361 FGSGRRKCPGLSFGIASFEFALANILYWFDWKLPGGCESLDVEETNGLTVRKKKSLHLNP 420
           FGSGRRKCPG+SFG+ASFE ALAN+L+WFDWKLP GCE L VEE  GLTV KK  L LNP
Sbjct: 453 FGSGRRKCPGMSFGVASFEQALANLLHWFDWKLPSGCE-LSVEEEGGLTVCKKIPLLLNP 506

Query: 421 IP 422
           IP
Sbjct: 513 IP 506

[Close]

BLAST of Chy12G218280 vs. ExPASy TrEMBL
Match: A0A6J1GU09 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456928 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 3.0e-174
Identity = 306/422 (72.51%), Postives = 356/422 (84.36%), Query Frame = 0


Query: 1   MAEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDS 60
           +A++VMKTHD+ FSNR QTTA+K L+Y CQD+ F  YGEYWRQA+K+C LELF+VKRVDS
Sbjct: 93  IAKEVMKTHDIKFSNRLQTTASKKLLYQCQDIAFVHYGEYWRQARKVCALELFSVKRVDS 152

Query: 61  FQYVRNEEIDALVNKILK-SGSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSRFEE 120
           FQYVR++E+  L+++I K S   ++++L  LF QTSNNIVSRCVLGEKFEDE+G SRF +
Sbjct: 153 FQYVRDDEVGGLMDRIRKASVCGEAVNLSQLFLQTSNNIVSRCVLGEKFEDENGNSRFGD 212

Query: 121 ISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKIKRS 180
            +RK MV++ AFC  D FPS  W ID+IRGF+ +LK+CFKTLD FFSKV+EEHKEKI RS
Sbjct: 213 TARKLMVLIVAFCAADLFPSLWW-IDIIRGFDKELKDCFKTLDTFFSKVLEEHKEKI-RS 272

Query: 181 GGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGLEWTM 240
           G + +    ESK DF+DIMLQLQQDD +DY+FS DNLKAI+LDMFVGGSDTTAT LEWTM
Sbjct: 273 GVLTD----ESKMDFLDIMLQLQQDDMVDYNFSSDNLKAIILDMFVGGSDTTATVLEWTM 332

Query: 241 TELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPRET 300
           TELM  P  MKK QEEVRTIIGKKSKIEAEDIQKMEY+ CVIKESLRLHPPVPLL+PRET
Sbjct: 333 TELMGNPAAMKKAQEEVRTIIGKKSKIEAEDIQKMEYIHCVIKESLRLHPPVPLLVPRET 392

Query: 301 MADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQNFEFIP 360
             DVEI+GY+IPSKTRVFVNAW IQRDP+ W NP EFIPERF+D   S DYKGQ++EFIP
Sbjct: 393 AEDVEIDGYHIPSKTRVFVNAWKIQRDPEYWKNPTEFIPERFID-NRSVDYKGQDYEFIP 452

Query: 361 FGSGRRKCPGLSFGIASFEFALANILYWFDWKLPGGCESLDVEETNGLTVRKKKSLHLNP 420
           FGSGRRKCPG+SFG+ASFE ALAN+L+WFDWKLP GCE L VEE +GLTVRKK  L LNP
Sbjct: 453 FGSGRRKCPGMSFGVASFEQALANLLHWFDWKLPSGCE-LSVEEEDGLTVRKKTPLILNP 506

Query: 421 IP 422
           +P
Sbjct: 513 MP 506

[Close]

BLAST of Chy12G218280 vs. ExPASy TrEMBL
Match: A0A6J1GRU2 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456926 PE=3 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 4.3e-173
Identity = 308/422 (72.99%), Postives = 350/422 (82.94%), Query Frame = 0


Query: 1   MAEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDS 60
           MA +VMKT D+ FSNRPQTTAA  L+Y CQD+GF  YGEYWRQA+K+C LELF+ KRVDS
Sbjct: 93  MAREVMKTQDIKFSNRPQTTAAYELLYQCQDIGFTQYGEYWRQARKVCALELFSAKRVDS 152

Query: 61  FQYVRNEEIDALVNKILKS-GSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSRFEE 120
           FQYVR++EI  L+++I K+    ++++L  LF QTSNNIVSRCVLGEKFEDE+G SRF +
Sbjct: 153 FQYVRDDEIGGLMDRIRKACVGVEAVNLSQLFLQTSNNIVSRCVLGEKFEDENGNSRFGD 212

Query: 121 ISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKIKRS 180
            +RK MV++ AFCV D FPS  W ID IRGFN  L++CFK LD FFSKVVEEHKEKI+  
Sbjct: 213 AARKVMVLMVAFCVADLFPSLWW-IDTIRGFNKQLEDCFKRLDTFFSKVVEEHKEKIR-- 272

Query: 181 GGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGLEWTM 240
            GV+     ESKKDFVDIMLQLQ+D  +DYHFS  NLKAIVLDMFVGGSDTTAT LEWTM
Sbjct: 273 DGVLT---DESKKDFVDIMLQLQRDGMVDYHFSPINLKAIVLDMFVGGSDTTATVLEWTM 332

Query: 241 TELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPRET 300
           TELMR P +MKK QEE+RTIIGKKSKIEAEDIQKMEYM  VI ESLRLHPP+PLL+PRET
Sbjct: 333 TELMRNPAVMKKAQEEIRTIIGKKSKIEAEDIQKMEYMHRVINESLRLHPPIPLLVPRET 392

Query: 301 MADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQNFEFIP 360
             DVEI+ Y+IPSKTRVFVNAW IQRDP+ W+NPNEFIPERFMD  NS DYKGQ++EFIP
Sbjct: 393 TTDVEIDSYHIPSKTRVFVNAWKIQRDPENWHNPNEFIPERFMD-NNSIDYKGQDYEFIP 452

Query: 361 FGSGRRKCPGLSFGIASFEFALANILYWFDWKLPGGCESLDVEETNGLTVRKKKSLHLNP 420
           FGSGRRKCPGLSFG ASFE ALAN+L+WFDWKLP  C +L VEE NGLTVRKK  L LNP
Sbjct: 453 FGSGRRKCPGLSFGAASFEHALANLLHWFDWKLPSDC-ALSVEEENGLTVRKKTPLLLNP 506

Query: 421 IP 422
           +P
Sbjct: 513 MP 506

[Close]

BLAST of Chy12G218280 vs. NCBI nr
Match: XP_031739470.1 (cytochrome P450 71A1-like [Cucumis sativus] >KAE8652604.1 hypothetical protein Csa_013720 [Cucumis sativus])

HSP 1 Score: 833 bits (2152), Expect = 5.36e-303
Identity = 403/426 (94.60%), Postives = 417/426 (97.89%), Query Frame = 0


Query: 1   MAEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDS 60
           MAEQVMKTHDLVFSNRPQTTAAKTL+YGCQD+GFAPYGEYWRQA+KIC LELF+VKRV+S
Sbjct: 82  MAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGEYWRQARKICALELFSVKRVES 141

Query: 61  FQYVRNEEIDALVNKILKSGSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSRFEEI 120
           FQYVR+EEID+LVNKI KSGSD SLDLGHLFFQTSNNIVS+CV+GEKFEDEDGKSRFEEI
Sbjct: 142 FQYVRDEEIDSLVNKIRKSGSDHSLDLGHLFFQTSNNIVSKCVMGEKFEDEDGKSRFEEI 201

Query: 121 SRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKIKRSG 180
           SRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDE FSKVVEEHKEKIKRSG
Sbjct: 202 SRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDELFSKVVEEHKEKIKRSG 261

Query: 181 GVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGLEWTMT 240
           GVINIDDYESKKDFVDIMLQLQQDDKLDY FSLDNLKAIVLDMF+GGSDTTATGLEWTMT
Sbjct: 262 GVINIDDYESKKDFVDIMLQLQQDDKLDYPFSLDNLKAIVLDMFIGGSDTTATGLEWTMT 321

Query: 241 ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPRETM 300
           ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDI+KMEYMQCVIKESLRLHPPVPLL+PRETM
Sbjct: 322 ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIKKMEYMQCVIKESLRLHPPVPLLVPRETM 381

Query: 301 ADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQNFEFIPF 360
           ADVEIEGYYIPSKTRVFVNAWAIQRDPQCW+NPNEFIPERFMDKTNS DYKGQNFEFIPF
Sbjct: 382 ADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNPNEFIPERFMDKTNSADYKGQNFEFIPF 441

Query: 361 GSGRRKCPGLSFGIASFEFALANILYWFDWKLPGGCESLDVEETNGLTVRKKKSLHLNPI 420
           GSGRRKCPGLSFGIASFEFALANILYWFDWKLP GCESLDVEE NGLTVRKKK LHL+PI
Sbjct: 442 GSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGCESLDVEEANGLTVRKKKPLHLSPI 501

Query: 421 PYVVSN 426
           PYVVSN
Sbjct: 502 PYVVSN 507

[Close]

BLAST of Chy12G218280 vs. NCBI nr
Match: XP_011651837.2 (cytochrome P450 71A1 [Cucumis sativus])

HSP 1 Score: 832 bits (2149), Expect = 1.54e-302
Identity = 405/426 (95.07%), Postives = 416/426 (97.65%), Query Frame = 0


Query: 1   MAEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDS 60
           +AEQVMKTHDLVFSNRPQTTAAKTL+YGCQDVGFAPYGEYWRQA+KIC LELF+VKRV+S
Sbjct: 82  IAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDVGFAPYGEYWRQARKICVLELFSVKRVES 141

Query: 61  FQYVRNEEIDALVNKILKSGSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSRFEEI 120
           FQYVR+EEID+L+NKI K GSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSRFEEI
Sbjct: 142 FQYVRDEEIDSLINKIRKIGSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSRFEEI 201

Query: 121 SRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKIKRSG 180
           SRKAMVIVTAFCVEDFFPSFGWIIDVIRGF+WDLKNCFKTLDEFFSKVVEEHKEKIKRSG
Sbjct: 202 SRKAMVIVTAFCVEDFFPSFGWIIDVIRGFHWDLKNCFKTLDEFFSKVVEEHKEKIKRSG 261

Query: 181 GVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGLEWTMT 240
           GVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSD+TATGLEWTMT
Sbjct: 262 GVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDSTATGLEWTMT 321

Query: 241 ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPRETM 300
           ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPRETM
Sbjct: 322 ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPRETM 381

Query: 301 ADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQNFEFIPF 360
           ADVEIEGYYIPSKTRVFVNAWAIQRDPQ W NPNEFIPERFMDKTNS DYKGQN EFIPF
Sbjct: 382 ADVEIEGYYIPSKTRVFVNAWAIQRDPQFWVNPNEFIPERFMDKTNSADYKGQNLEFIPF 441

Query: 361 GSGRRKCPGLSFGIASFEFALANILYWFDWKLPGGCESLDVEETNGLTVRKKKSLHLNPI 420
           GSGRRKCPGLSF IASFEF LANILYWFDWKLP GCESLDVEE+NGLTVRKKKSLHLNPI
Sbjct: 442 GSGRRKCPGLSFAIASFEFVLANILYWFDWKLPDGCESLDVEESNGLTVRKKKSLHLNPI 501

Query: 421 PYVVSN 426
           PYVVSN
Sbjct: 502 PYVVSN 507

[Close]

BLAST of Chy12G218280 vs. NCBI nr
Match: KAE8652601.1 (hypothetical protein Csa_013149, partial [Cucumis sativus])

HSP 1 Score: 832 bits (2149), Expect = 2.07e-302
Identity = 405/426 (95.07%), Postives = 416/426 (97.65%), Query Frame = 0


Query: 1   MAEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDS 60
           +AEQVMKTHDLVFSNRPQTTAAKTL+YGCQDVGFAPYGEYWRQA+KIC LELF+VKRV+S
Sbjct: 90  IAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDVGFAPYGEYWRQARKICVLELFSVKRVES 149

Query: 61  FQYVRNEEIDALVNKILKSGSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSRFEEI 120
           FQYVR+EEID+L+NKI K GSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSRFEEI
Sbjct: 150 FQYVRDEEIDSLINKIRKIGSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSRFEEI 209

Query: 121 SRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKIKRSG 180
           SRKAMVIVTAFCVEDFFPSFGWIIDVIRGF+WDLKNCFKTLDEFFSKVVEEHKEKIKRSG
Sbjct: 210 SRKAMVIVTAFCVEDFFPSFGWIIDVIRGFHWDLKNCFKTLDEFFSKVVEEHKEKIKRSG 269

Query: 181 GVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGLEWTMT 240
           GVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSD+TATGLEWTMT
Sbjct: 270 GVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDSTATGLEWTMT 329

Query: 241 ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPRETM 300
           ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPRETM
Sbjct: 330 ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPRETM 389

Query: 301 ADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQNFEFIPF 360
           ADVEIEGYYIPSKTRVFVNAWAIQRDPQ W NPNEFIPERFMDKTNS DYKGQN EFIPF
Sbjct: 390 ADVEIEGYYIPSKTRVFVNAWAIQRDPQFWVNPNEFIPERFMDKTNSADYKGQNLEFIPF 449

Query: 361 GSGRRKCPGLSFGIASFEFALANILYWFDWKLPGGCESLDVEETNGLTVRKKKSLHLNPI 420
           GSGRRKCPGLSF IASFEF LANILYWFDWKLP GCESLDVEE+NGLTVRKKKSLHLNPI
Sbjct: 450 GSGRRKCPGLSFAIASFEFVLANILYWFDWKLPDGCESLDVEESNGLTVRKKKSLHLNPI 509

Query: 421 PYVVSN 426
           PYVVSN
Sbjct: 510 PYVVSN 515

[Close]

BLAST of Chy12G218280 vs. NCBI nr
Match: XP_031739495.1 (cytochrome P450 71A1-like [Cucumis sativus])

HSP 1 Score: 828 bits (2140), Expect = 3.61e-301
Identity = 401/426 (94.13%), Postives = 416/426 (97.65%), Query Frame = 0


Query: 1   MAEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDS 60
           MAEQVMKTHDLVFSNRPQTTAAKTL+YGCQD+GFAPYGEYWRQA+KIC LELF+VKRV+S
Sbjct: 82  MAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGEYWRQARKICALELFSVKRVES 141

Query: 61  FQYVRNEEIDALVNKILKSGSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSRFEEI 120
           FQYVR+EEID+LVNKI KSGSD SLDLGHLFF+TSNNIVSRCV+GEKFE+EDGKSRFEEI
Sbjct: 142 FQYVRDEEIDSLVNKIRKSGSDGSLDLGHLFFRTSNNIVSRCVMGEKFENEDGKSRFEEI 201

Query: 121 SRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKIKRSG 180
           SRK MVI+TAFC EDFFPSFGWIIDVIRGF+WDLKNCFKTLDEFFSKVVEEHKEKIKRSG
Sbjct: 202 SRKVMVIITAFCFEDFFPSFGWIIDVIRGFHWDLKNCFKTLDEFFSKVVEEHKEKIKRSG 261

Query: 181 GVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGLEWTMT 240
           GVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGLEWTMT
Sbjct: 262 GVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGLEWTMT 321

Query: 241 ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPRETM 300
           ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDI+KMEYMQCVIKESLRLHPPVPLL+PRETM
Sbjct: 322 ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIKKMEYMQCVIKESLRLHPPVPLLVPRETM 381

Query: 301 ADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQNFEFIPF 360
           ADVEIEGYYIPSKTRVFVNAWAIQRDPQ W+NPNEFIPERFMDKTNS DYKGQNFEFIPF
Sbjct: 382 ADVEIEGYYIPSKTRVFVNAWAIQRDPQFWDNPNEFIPERFMDKTNSADYKGQNFEFIPF 441

Query: 361 GSGRRKCPGLSFGIASFEFALANILYWFDWKLPGGCESLDVEETNGLTVRKKKSLHLNPI 420
           GSGRRKCPGLSFGIASFEFALANILYWFDWKLP GCESLDVEE NGLTVRKKK LHLNPI
Sbjct: 442 GSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGCESLDVEEANGLTVRKKKPLHLNPI 501

Query: 421 PYVVSN 426
           PYVVSN
Sbjct: 502 PYVVSN 507

[Close]

BLAST of Chy12G218280 vs. NCBI nr
Match: XP_031738835.1 (cytochrome P450 71A1-like isoform X1 [Cucumis sativus])

HSP 1 Score: 783 bits (2021), Expect = 1.96e-282
Identity = 382/415 (92.05%), Postives = 398/415 (95.90%), Query Frame = 0


Query: 1   MAEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDS 60
           MA+QVMKTHDLVFS+RPQTTA KTL+YG QDVGFAPYGEYWRQAKKICTLEL +VKRV+S
Sbjct: 82  MAKQVMKTHDLVFSSRPQTTAVKTLLYGGQDVGFAPYGEYWRQAKKICTLELLSVKRVES 141

Query: 61  FQYVRNEEIDALVNKILKSGSDQSLDLGHLFFQTSNNIVSRCVLGEKFEDEDGKSRFEEI 120
           FQYVR+EEIDALVNKI KSGSDQSLDLGHLFFQTSNNIV+RCV+GEKFEDEDGKSRFEEI
Sbjct: 142 FQYVRDEEIDALVNKIRKSGSDQSLDLGHLFFQTSNNIVARCVMGEKFEDEDGKSRFEEI 201

Query: 121 SRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKIKRSG 180
           SRK +VIVTAFCVEDFFPSFGWIIDVIRGF+WDLKNCFKTLDEF SKVVEEHKEKIKRSG
Sbjct: 202 SRKVVVIVTAFCVEDFFPSFGWIIDVIRGFHWDLKNCFKTLDEFLSKVVEEHKEKIKRSG 261

Query: 181 GVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATGLEWTMT 240
           G INID+YESKKDFVDIMLQLQQ D+LDYHFSLD+LKAIV DMF+GGSDTTATGLEWTMT
Sbjct: 262 GDINIDNYESKKDFVDIMLQLQQGDELDYHFSLDSLKAIVSDMFIGGSDTTATGLEWTMT 321

Query: 241 ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPRETM 300
           ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLL+PRETM
Sbjct: 322 ELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLVPRETM 381

Query: 301 ADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQNFEFIPF 360
           ADVEIEGYYIPSKTRVFVNAWAIQRDPQ W NPNEFIPERFMDKTNS DYKGQNFEFIPF
Sbjct: 382 ADVEIEGYYIPSKTRVFVNAWAIQRDPQFWVNPNEFIPERFMDKTNSADYKGQNFEFIPF 441

Query: 361 GSGRRKCPGLSFGIASFEFALANILYWFDWKLPGGCESLDVEETNGLTVRKKKSL 415
           GSGRRKC GLSFGIASFEFALANIL WFDWKLPGGCESLD+EE NGLTVRKK  L
Sbjct: 442 GSGRRKCAGLSFGIASFEFALANILCWFDWKLPGGCESLDIEEANGLTVRKKNQL 496

[Close]

BLAST of Chy12G218280 vs. TAIR 10
Match: AT3G26210.1 (cytochrome P450, family 71, subfamily B, polypeptide 23 )

HSP 1 Score: 374.8 bits (961), Expect = 9.3e-104
Identity = 194/429 (45.22%), Postives = 278/429 (64.80%), Query Frame = 0


Query: 2   AEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDSF 61
           AE+V+KTHDL   +RP+T A+KT+ Y  +D+GFAPYGE WR  +K+  +ELF++K+ +SF
Sbjct: 84  AEEVLKTHDLDCCSRPETIASKTISYNFKDIGFAPYGEEWRALRKLAVIELFSLKKFNSF 143

Query: 62  QYVRNEEIDALVNKILKSGSDQS-LDLGHLFFQTSNNIVSRCVLGE-----KFEDEDGKS 121
           +Y+R EE D LV K+ ++   QS ++L    F  S +IV R   G+     +F DED   
Sbjct: 144 RYIREEENDLLVKKLSEASEKQSPVNLKKALFTLSASIVCRLAFGQNLHESEFIDEDS-- 203

Query: 122 RFEEISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEK 181
             E+++ ++  I   F   +FFP  GWI+D I G +  L   F  LD FF++V+++H   
Sbjct: 204 -MEDLASRSEKIQAKFAFSNFFPG-GWILDKITGQSKSLNEIFADLDGFFNQVLDDH--- 263

Query: 182 IKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSL--DNLKAIVLDMFVGGSDTTAT 241
           +K    V+         D VD+M+ +      D  F L  D++K I+ D+F+ G +T+AT
Sbjct: 264 LKPGRKVL------ETPDVVDVMIDMMNKQSQDGSFKLTTDHIKGIISDIFLAGVNTSAT 323

Query: 242 GLEWTMTELMRKPRIMKKVQEEVRTIIG-KKSKIEAEDIQKMEYMQCVIKESLRLHPPVP 301
            + W MTEL+R PR+MKKVQ+EVRT++G K+ +I  +D+ ++ Y + VIKE+ RLHP  P
Sbjct: 324 TILWAMTELIRNPRVMKKVQDEVRTVLGEKRDRITEQDLNQLNYFKLVIKETFRLHPAAP 383

Query: 302 LLLPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKG 361
           LLLPRE MA ++I+GY IP KT++ VN +AI RDP  W NP EF PERF+D  +S DY+G
Sbjct: 384 LLLPREAMAKIKIQGYDIPEKTQIMVNVYAIGRDPDLWENPEEFKPERFVD--SSVDYRG 443

Query: 362 QNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPGG--CESLDVEETNGLTVR 420
            NFE +PFGSGRR CPG++ GIA+ E  L N+LY+FDW LP G   + +D+EE   + + 
Sbjct: 444 LNFELLPFGSGRRICPGMTMGIATVELGLLNLLYFFDWGLPEGRTVKDIDLEEEGAIIIG 497

[Close]

BLAST of Chy12G218280 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 367.5 bits (942), Expect = 1.5e-101
Identity = 183/429 (42.66%), Postives = 276/429 (64.34%), Query Frame = 0


Query: 2   AEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDSF 61
           AE V+KT+DL   +RP+T  +  L YG +D+ FAPYGEYWR+ +K+  +ELF+ K+V SF
Sbjct: 84  AEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYWREVRKLAVIELFSSKKVQSF 143

Query: 62  QYVRNEEIDALVNKILKSGSDQS-LDLGHLFFQTSNNIVSRCVLGEKFEDED---GKSRF 121
           +Y+R EE+D +V K+ +S   QS +DL   FF  + +I+ R  LG+ F +      + R 
Sbjct: 144 RYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASIICRVALGQNFNESGFVIDQDRI 203

Query: 122 EEISRKAMVIVTAFCVEDFFP-SFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKI 181
           EE+  ++   +  F   DFFP   G  +D +   +  +   FK LD F+  V+++H   +
Sbjct: 204 EELVTESAEALGTFTFSDFFPGGLGRFVDWLFQRHKKINKVFKELDAFYQHVIDDH---L 263

Query: 182 KRSGGVINIDDYESKKDFVDIMLQL--QQDDKLDYHFSLDNLKAIVLDMFVGGSDTTATG 241
           K  G           +D V ++L +  +Q+D   +  ++DNLKAIV+D+F+ G DT+A  
Sbjct: 264 KPEG--------RKNQDIVTLILDMIDKQEDSDSFKLNMDNLKAIVMDVFLAGIDTSAVT 323

Query: 242 LEWTMTELMRKPRIMKKVQEEVRTIIG-KKSKIEAEDIQKMEYMQCVIKESLRLHPPVPL 301
           + W MTEL+R PR+MKK QE +RT +G KK +I  ED+ K+EY+  ++KE+ RLHP +P 
Sbjct: 324 MIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVEYLNHILKETFRLHPALPF 383

Query: 302 LLPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQ 361
           ++PRETM+ ++I+GY IP KT++ +N W I RDP+ WN+P EF PERF +  +S D++GQ
Sbjct: 384 VVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWNDPEEFNPERFAN--SSVDFRGQ 443

Query: 362 NFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPGGC--ESLDVEETNGLTVRK 421
           +F+ +PFGSGRR CPG+   IAS E AL N+LY+FDW +P G   E +D+EE   +++ K
Sbjct: 444 HFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSMPDGTKGEDIDMEEAGNISIVK 499

[Close]

BLAST of Chy12G218280 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 362.5 bits (929), Expect = 4.8e-100
Identity = 183/424 (43.16%), Postives = 267/424 (62.97%), Query Frame = 0


Query: 2   AEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDSF 61
           A QV++ HDL    RP  +  + L Y   D+ F+PY +YW++ +K+C  ELF+ K+V S 
Sbjct: 83  ARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAFSPYDDYWKEVRKLCVQELFSTKQVHSI 142

Query: 62  QYVRNEEIDALVNKILKSGSDQS-LDLGHLFFQTSNNIVSRCVLGEKFEDED-GKSRFEE 121
           Q +++EE+  +++ I +S S ++ ++L +   + + ++V R   G  FE       RF +
Sbjct: 143 QPIKDEEVKKMIDSIAESASQKNPVNLNNKCLELTVSVVCRTAFGVSFEGTVLNSDRFNK 202

Query: 122 ISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKIKRS 181
           I R+A+ ++ +F   DF P  GWIIDV+ G     +   + L+ FF ++ + HKE  K  
Sbjct: 203 IVREALEMLGSFSAADFIPYVGWIIDVLTGLQGRRERSKRDLNAFFEQMFDLHKEGKK-- 262

Query: 182 GGVINIDDYESKKDFVDIMLQLQQDDKL--DYHFSLDNLKAIVLDMFVGGSDTTATGLEW 241
                    E  +DFVD++L+L++++ +  +   + +++KAI+LD+ + G DT+A  + W
Sbjct: 263 ---------EGNEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILLDVLLAGIDTSAITMTW 322

Query: 242 TMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLLLPR 301
            MTEL R PR+MKKVQ E+RT +G +S I  ED+ ++EY++ VIKE+ RLHP  PLLLPR
Sbjct: 323 AMTELARNPRVMKKVQSEIRTQMGNRSMISFEDMDQLEYLKMVIKETWRLHPTTPLLLPR 382

Query: 302 ETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQNFEF 361
           E M++ +I GY IP KTR+ VN WAI RDP  W +P  F+PERFMD  N+ D KGQ+FE 
Sbjct: 383 EAMSEFDINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFMD--NNIDAKGQHFEL 442

Query: 362 IPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPGGCE--SLDVEETNGLTVRKKKSL 420
           +PFG GRR CP +  G    EF LAN+LY FDWKLP G E   +DVEE  GLTV KK  L
Sbjct: 443 LPFGGGRRICPAIYMGTTMVEFGLANLLYHFDWKLPEGVEVKDIDVEEAPGLTVNKKNEL 493

[Close]

BLAST of Chy12G218280 vs. TAIR 10
Match: AT3G26200.1 (cytochrome P450, family 71, subfamily B, polypeptide 22 )

HSP 1 Score: 357.1 bits (915), Expect = 2.0e-98
Identity = 178/430 (41.40%), Postives = 269/430 (62.56%), Query Frame = 0


Query: 2   AEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDSF 61
           AE+V+KTHDL    RP+ TA K   Y  +D+GFA YG+ WR+ +K+  LELF+ K++ +F
Sbjct: 81  AEEVLKTHDLETCTRPKLTATKLFSYNYKDIGFAQYGDDWREMRKLAMLELFSSKKLKAF 140

Query: 62  QYVRNEEIDALVNKILKSGSDQSL-DLGHLFFQTSNNIVSRCVLGEKFEDED--GKSRFE 121
           +Y+R EE + LVNK+ KS   +++ DL    F  + +IV R   G+ F + D     + E
Sbjct: 141 RYIREEESEVLVNKLSKSAETRTMVDLRKALFSYTASIVCRLAFGQNFHECDFVDMDKVE 200

Query: 122 EISRKAMVIVTAFCVEDFFPS-FGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEKIK 181
           ++  ++   + +F   DFFP+  GW+ID I G + +L   F  L  FF  V+++H     
Sbjct: 201 DLVLESETNLGSFAFTDFFPAGLGWVIDRISGQHSELHKAFARLSNFFQHVIDDH----- 260

Query: 182 RSGGVINIDDYESKKDFVDIML-QLQQDDKL-DYHFSLDNLKAIVLDMFVGGSDTTATGL 241
                +     +   D + +ML  + ++ K+  +  + D+LK ++ D+F+ G +  A  +
Sbjct: 261 -----LKPGQSQDHSDIIGVMLDMINKESKVGSFQVTYDHLKGVMSDVFLAGVNAGAITM 320

Query: 242 EWTMTELMRKPRIMKKVQEEVRTIIG-KKSKIEAEDIQKMEYMQCVIKESLRLHPPVPLL 301
            W MTEL R PR+MKK+Q+E+R I+G  K KI  +D++K+ Y++ VI+E+ RLHPP PLL
Sbjct: 321 IWAMTELARHPRVMKKLQQEIREILGDNKEKITEQDLEKVHYLKLVIEETFRLHPPAPLL 380

Query: 302 LPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYKGQN 361
           LPRETM+D++I+GY IP  T + +N ++I RDP CW NPN+F PERF+D  +  +YKGQ+
Sbjct: 381 LPRETMSDLKIQGYNIPKNTMIEINTYSIGRDPNCWENPNDFNPERFID--SPVEYKGQH 440

Query: 362 FEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPGG--CESLDVEETNGLTVRKK 421
           +E +PFG+GRR CPG++ GI   E  L N+LY+FDW LP G   E +D+EE     V KK
Sbjct: 441 YELLPFGAGRRICPGMATGITIVELGLLNVLYFFDWSLPDGMKIEDIDMEEAGAFVVAKK 498

Query: 422 KSLHLNPIPY 423
             L L P P+
Sbjct: 501 VPLELIPTPH 498

[Close]

BLAST of Chy12G218280 vs. TAIR 10
Match: AT1G13110.1 (cytochrome P450, family 71 subfamily B, polypeptide 7 )

HSP 1 Score: 356.7 bits (914), Expect = 2.6e-98
Identity = 183/431 (42.46%), Postives = 267/431 (61.95%), Query Frame = 0


Query: 2   AEQVMKTHDLVFSNRPQTTAAKTLVYGCQDVGFAPYGEYWRQAKKICTLELFTVKRVDSF 61
           AE+ +KT DL   +RP+T A + + Y  +D+GFAPYGE W+  +K+  +EL   K+  SF
Sbjct: 85  AEEALKTQDLECCSRPETVATRMISYNFKDIGFAPYGEEWKALRKLVVMELLNTKKFQSF 144

Query: 62  QYVRNEEIDALVNKILKSGSDQS-LDLGHLFFQTSNNIVSRCVLGE-----KFEDEDGKS 121
           +Y+R EE D L+ K+ +S   +S ++L    F    +IV R   G      +F DED  +
Sbjct: 145 RYIREEENDLLIKKLTESALKKSPVNLKKTLFTLVASIVCRLAFGVNIHKCEFVDEDNVA 204

Query: 122 RFEEISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEFFSKVVEEHKEK 181
              ++  K  ++V      DFFP  GW++D I G N  L N F  LD FF  V+++H   
Sbjct: 205 ---DLVNKFEMLVAGVAFTDFFPGVGWLVDRISGQNKTLNNVFSELDTFFQNVLDDH--- 264

Query: 182 IKRSGGVINIDDYESKKDFVDIMLQL---QQDDKLDYHFSLDNLKAIVLDMFVGGSDTTA 241
           IK    V          D VD+ML L   Q+ D   +  + D+LK I+ D+F+ G +T+A
Sbjct: 265 IKPGRQV------SENPDVVDVMLDLMKKQEKDGESFKLTTDHLKGIISDIFLAGVNTSA 324

Query: 242 TGLEWTMTELMRKPRIMKKVQEEVRTIIG-KKSKIEAEDIQKMEYMQCVIKESLRLHPPV 301
             L W M EL+R PR+MKKVQ+E+RT +G KK +I  +D+ ++ Y + V+KE  RLHP  
Sbjct: 325 VTLNWAMAELIRNPRVMKKVQDEIRTTLGDKKQRITEQDLSQVHYFKLVVKEIFRLHPAA 384

Query: 302 PLLLPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWNNPNEFIPERFMDKTNSTDYK 361
           PLLLPRETM+ V+I+GY IP KT++ +N ++I RDP+ W NP+EF P+RF+D  +S DY+
Sbjct: 385 PLLLPRETMSHVKIQGYDIPVKTQMMINIYSIARDPKLWTNPDEFNPDRFLD--SSIDYR 444

Query: 362 GQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPGG--CESLDVEETNGLTV 421
           G NFE +PFGSGRR CPG++ GI + E  L N+LY+FDW +P G   + +++EET  + +
Sbjct: 445 GLNFELLPFGSGRRICPGMTLGITTVELGLLNLLYFFDWVVPVGKNVKDINLEETGSIII 501

The following BLAST results are available for this feature:

BLAST of Chy12G218280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q609	4.1e-128	53.15	Phenylacetaldehyde oxime monooxygenase CYP71AN24 OS=Prunus mume OX=102107 GN=CYP...	[more]
P24465	9.7e-122	48.46	Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2	[more]
A0A0N9HTU1	1.1e-106	44.08	Desmethylyatein synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71CU1 PE=1 ...	[more]
Q9LTM0	1.3e-102	45.22	Cytochrome P450 71B23 OS=Arabidopsis thaliana OX=3702 GN=CYP71B23 PE=2 SV=1	[more]
A0A0N9HT29	3.8e-102	44.89	Desmethyl-deoxy-podophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 G...	[more]

BLAST of Chy12G218280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C631	1.9e-176	72.20	cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008616 PE=3 SV...	[more]
A0A6J1C5L5	5.4e-176	72.88	cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008617 PE=3 SV...	[more]
A0A6J1GRT5	7.8e-175	73.46	cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456925 PE=3 SV=...	[more]
A0A6J1GU09	3.0e-174	72.51	cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456928 PE=3 SV=...	[more]
A0A6J1GRU2	4.3e-173	72.99	cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456926 PE=3 SV=...	[more]

BLAST of Chy12G218280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739470.1	5.36e-303	94.60	cytochrome P450 71A1-like [Cucumis sativus] >KAE8652604.1 hypothetical protein C...	[more]
XP_011651837.2	1.54e-302	95.07	cytochrome P450 71A1 [Cucumis sativus]	[more]
KAE8652601.1	2.07e-302	95.07	hypothetical protein Csa_013149, partial [Cucumis sativus]	[more]
XP_031739495.1	3.61e-301	94.13	cytochrome P450 71A1-like [Cucumis sativus]	[more]
XP_031738835.1	1.96e-282	92.05	cytochrome P450 71A1-like isoform X1 [Cucumis sativus]	[more]

BLAST of Chy12G218280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26210.1	9.3e-104	45.22	cytochrome P450, family 71, subfamily B, polypeptide 23	[more]
AT1G13080.1	1.5e-101	42.66	cytochrome P450, family 71, subfamily B, polypeptide 2	[more]
AT3G26310.1	4.8e-100	43.16	cytochrome P450, family 71, subfamily B, polypeptide 35	[more]
AT3G26200.1	2.0e-98	41.40	cytochrome P450, family 71, subfamily B, polypeptide 22	[more]
AT1G13110.1	2.6e-98	42.46	cytochrome P450, family 71 subfamily B, polypeptide 7	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 249..269
None	No IPR available	PANTHER	PTHR47956:SF10	CYTOCHROME P450 FAMILY 71 PROTEIN	coord: 1..421
None	No IPR available	PANTHER	PTHR47956	CYTOCHROME P450 71B11-RELATED	coord: 1..421
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 358..367 score: 57.61 coord: 226..243 score: 36.64 coord: 279..290 score: 45.15
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 1..413 e-value: 2.4E-95 score: 320.0
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 367..390 score: 24.64 coord: 278..296 score: 39.47 coord: 319..343 score: 39.01 coord: 94..112 score: 23.88 coord: 357..367 score: 56.72 coord: 215..232 score: 38.66 coord: 1..18 score: 19.5 coord: 235..261 score: 34.91
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..420 e-value: 9.8E-112 score: 375.9
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 2..420
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 360..369

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G218280.1	Chy12G218280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen