Chy12G214890 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G214890
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	DNA-directed RNA polymerase subunit
Location	chrH12: 14265887 .. 14266816 (+)
RNA-Seq Expression	Chy12G214890
Synteny	Chy12G214890

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCACTGAGGGAGTTGACGGGTGTAACACAAAGAGTAATCACATTATTGAAGTGCAACAAGTACTTGGCATTGAAGCTGCAAGGAAATGTATTATTGAAGAGATAAAATATACCATGGAAAGTCATGGAATGAGCATAGACATACGTCATATGATGCTTTTAGGAGATTTGATGACATTCAGGGTAAGTAAGTTGTGTTGGGTTACAAAAAATTACATGTGTATAGGTTGAGGCAATTAAATATTTTTGCTGTGTGACAGGGTGAAGTTCTTGGAATCACAAGATTTGGCATTCAAAAAATGGATAAAAGTGTACTGATGCTTGCTTCATTTGAGAAAACCGCGGATCATCTTTTTAATGCTTCTGTGAATGGAAGGGATGACAAAATTGAAGGAGTTAGTGAATGCGTAATCATGGGTATACCAATGCCGCTAGGCACTGGCATGTTAAAAGTTAGACAAAGGTATATCTTCTCCTACTGGAACAGAACTCAGTTCATTATTAATATTATTTGAGCATTGATTTCATTGGCAGCATCTGACGAACGTTTTTCTTAATGCCTGGAGTGTGATTTAACATACTTTTATGAGCAATTAGCTGAGAGAATTCTGTGTTCAAATTCCTACAATGTTATTTACTCTCCACTAATATTGATTTACACTTATTGAAACTTCTAATATTTCAAGCCTACAAGTGAGGACAGTGTTAGATGATATAATATTAGATTTACATTCATCCATCAGTTTAAGTTTTTGAGTCAATTCATGATTCAGGTTCAACATTTTGGTTGAGTATTAACATCAACAAATAATTTTGCATATTACAAACTTTAAAGTTTTCATATTTTGAAGCTAATTGAGCTTTGTTTTTAACAGAGTTTCTGTGCCACAACAATTACCTTATGGACCACCTGCAATTCTATCTTGA

mRNA sequence

ATGGGCACTGAGGGAGTTGACGGGTGTAACACAAAGAGTAATCACATTATTGAAGTGCAACAAGTACTTGGCATTGAAGCTGCAAGGAAATGTATTATTGAAGAGATAAAATATACCATGGAAAGTCATGGAATGAGCATAGACATACGTCATATGATGCTTTTAGGAGATTTGATGACATTCAGGGGTGAAGTTCTTGGAATCACAAGATTTGGCATTCAAAAAATGGATAAAAGTGTACTGATGCTTGCTTCATTTGAGAAAACCGCGGATCATCTTTTTAATGCTTCTGTGAATGGAAGGGATGACAAAATTGAAGGAGTTAGTGAATGCGTAATCATGGGTATACCAATGCCGCTAGGCACTGGCATGTTAAAAGTTAGACAAAGAGTTTCTGTGCCACAACAATTACCTTATGGACCACCTGCAATTCTATCTTGA

Coding sequence (CDS)

ATGGGCACTGAGGGAGTTGACGGGTGTAACACAAAGAGTAATCACATTATTGAAGTGCAACAAGTACTTGGCATTGAAGCTGCAAGGAAATGTATTATTGAAGAGATAAAATATACCATGGAAAGTCATGGAATGAGCATAGACATACGTCATATGATGCTTTTAGGAGATTTGATGACATTCAGGGGTGAAGTTCTTGGAATCACAAGATTTGGCATTCAAAAAATGGATAAAAGTGTACTGATGCTTGCTTCATTTGAGAAAACCGCGGATCATCTTTTTAATGCTTCTGTGAATGGAAGGGATGACAAAATTGAAGGAGTTAGTGAATGCGTAATCATGGGTATACCAATGCCGCTAGGCACTGGCATGTTAAAAGTTAGACAAAGAGTTTCTGTGCCACAACAATTACCTTATGGACCACCTGCAATTCTATCTTGA

Protein sequence

MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS*

Homology

[Close]

BLAST of Chy12G214890 vs. ExPASy Swiss-Prot
Match: F4JXF9 (DNA-directed RNA polymerase III subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPC1 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 1.1e-48
Identity = 97/146 (66.44%), Postives = 116/146 (79.45%), Query Frame = 0


Query: 1    MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
            MGT G++G  T SN+++EV + LGIEAAR  II+EI   M +HGMSIDIRHMMLL D+MT
Sbjct: 1231 MGTPGINGRTTTSNNVVEVSKTLGIEAARTTIIDEIGTVMGNHGMSIDIRHMMLLADVMT 1290

Query: 61   FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
            +RGEVLGI R GIQKMDKSVLM ASFE+T DHLF+A+ +G+ D IEGV+ECVIMGIPM L
Sbjct: 1291 YRGEVLGIQRTGIQKMDKSVLMQASFERTGDHLFSAAASGKVDNIEGVTECVIMGIPMKL 1350

Query: 121  GTGMLKVRQRVSVPQQLPYGPPAILS 147
            GTG+LKV QR     +L YGP  I+S
Sbjct: 1351 GTGILKVLQRTDDLPKLKYGPDPIIS 1376

[Close]

BLAST of Chy12G214890 vs. ExPASy Swiss-Prot
Match: Q5ZL98 (DNA-directed RNA polymerase III subunit RPC1 OS=Gallus gallus OX=9031 GN=POLR3A PE=2 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 5.3e-43
Identity = 87/141 (61.70%), Postives = 106/141 (75.18%), Query Frame = 0


Query: 1    MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
            M T GV G  T SN+  EV++ LGIEAAR  II EI+YTM +HGMSID RH+MLL DLMT
Sbjct: 1236 MATHGVKGTKTSSNNTYEVEKTLGIEAARTTIINEIQYTMVNHGMSIDRRHVMLLSDLMT 1295

Query: 61   FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
            ++GEVLGITRFG+ KM +SVLMLASFEKTADHLF+A+  G+ D + GVSEC+IMGIPM +
Sbjct: 1296 YKGEVLGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMGIPMNI 1355

Query: 121  GTGMLKVRQRVSVPQQLPYGP 142
            GTG+ K+  +       P  P
Sbjct: 1356 GTGLFKLLHKADKESTPPRRP 1376

[Close]

BLAST of Chy12G214890 vs. ExPASy Swiss-Prot
Match: A4IF62 (DNA-directed RNA polymerase III subunit RPC1 OS=Bos taurus OX=9913 GN=POLR3A PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 6.9e-43
Identity = 87/141 (61.70%), Postives = 107/141 (75.89%), Query Frame = 0


Query: 1    MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
            M T GV G  T SN+  EV++ LGIEAAR  II EI+YTM +HGMSID RH+MLL DLMT
Sbjct: 1236 MATHGVKGTRTTSNNTYEVEKTLGIEAARTTIINEIQYTMVNHGMSIDRRHVMLLSDLMT 1295

Query: 61   FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
            ++GEVLGITRFG+ KM +SVLMLASFEKTADHLF+A+  G+ D + GVSEC+IMGIPM +
Sbjct: 1296 YKGEVLGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMGIPMNI 1355

Query: 121  GTGMLKVRQRVSVPQQLPYGP 142
            GTG+ K+  + +     P  P
Sbjct: 1356 GTGLFKLLHKANRDPSPPRRP 1376

[Close]

BLAST of Chy12G214890 vs. ExPASy Swiss-Prot
Match: O14802 (DNA-directed RNA polymerase III subunit RPC1 OS=Homo sapiens OX=9606 GN=POLR3A PE=1 SV=2)

HSP 1 Score: 174.5 bits (441), Expect = 9.0e-43
Identity = 87/141 (61.70%), Postives = 106/141 (75.18%), Query Frame = 0


Query: 1    MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
            M T GV G  T SN+  EV++ LGIEAAR  II EI+YTM +HGMSID RH+MLL DLMT
Sbjct: 1236 MATHGVKGTRTTSNNTYEVEKTLGIEAARTTIINEIQYTMVNHGMSIDRRHVMLLSDLMT 1295

Query: 61   FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
            ++GEVLGITRFG+ KM +SVLMLASFEKTADHLF+A+  G+ D + GVSEC+IMGIPM +
Sbjct: 1296 YKGEVLGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMGIPMNI 1355

Query: 121  GTGMLKVRQRVSVPQQLPYGP 142
            GTG+ K+  +       P  P
Sbjct: 1356 GTGLFKLLHKADRDPNPPKRP 1376

[Close]

BLAST of Chy12G214890 vs. ExPASy Swiss-Prot
Match: Q86AQ5 (DNA-directed RNA polymerase III subunit rpc1 OS=Dictyostelium discoideum OX=44689 GN=polr3a PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 2.6e-42
Identity = 83/130 (63.85%), Postives = 103/130 (79.23%), Query Frame = 0


Query: 1    MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
            M T G+ G +T SNHI+E +  LGIE AR  I+ EI   M SHGMSIDIRH+MLL DLM+
Sbjct: 1306 MATPGIKGTHTTSNHIMECENTLGIETARNTIMSEIDMIMTSHGMSIDIRHVMLLADLMS 1365

Query: 61   FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
            F+GE+LGITRFGI KM +SVLMLASFEKT DHLF+A+V+ R D I GVSEC+IMG+ +PL
Sbjct: 1366 FKGEILGITRFGIAKMKESVLMLASFEKTTDHLFDAAVHHRQDDIVGVSECIIMGVVIPL 1425

Query: 121  GTGMLKVRQR 131
            GTG+ K+ ++
Sbjct: 1426 GTGLFKLLRK 1435

[Close]

BLAST of Chy12G214890 vs. ExPASy TrEMBL
Match: A0A0A0LXJ4 (DNA-directed RNA polymerase OS=Cucumis sativus OX=3659 GN=Csa_1G083760 PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 2.2e-76
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 1   MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
           MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT
Sbjct: 42  MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 101

Query: 61  FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
           FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL
Sbjct: 102 FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 161

Query: 121 GTGMLKVRQRVSVPQQLPYGPPAILS 147
           GTGMLKVRQRVSVPQQLPYGPPAILS
Sbjct: 162 GTGMLKVRQRVSVPQQLPYGPPAILS 187

[Close]

BLAST of Chy12G214890 vs. ExPASy TrEMBL
Match: A0A5A7T0K7 (DNA-directed RNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold359G00430 PE=4 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 6.4e-76
Identity = 145/146 (99.32%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 1   MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
           MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT
Sbjct: 114 MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 173

Query: 61  FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
           FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL
Sbjct: 174 FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 233

Query: 121 GTGMLKVRQRVSVPQQLPYGPPAILS 147
           GTGMLKVRQRVSVP+QLPYGPPAILS
Sbjct: 234 GTGMLKVRQRVSVPRQLPYGPPAILS 259

[Close]

BLAST of Chy12G214890 vs. ExPASy TrEMBL
Match: A0A1S3CNX9 (DNA-directed RNA polymerase subunit OS=Cucumis melo OX=3656 GN=LOC103502942 PE=3 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 6.4e-76
Identity = 145/146 (99.32%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 1    MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
            MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT
Sbjct: 1255 MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 1314

Query: 61   FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
            FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL
Sbjct: 1315 FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 1374

Query: 121  GTGMLKVRQRVSVPQQLPYGPPAILS 147
            GTGMLKVRQRVSVP+QLPYGPPAILS
Sbjct: 1375 GTGMLKVRQRVSVPRQLPYGPPAILS 1400

[Close]

BLAST of Chy12G214890 vs. ExPASy TrEMBL
Match: A0A6J1I0L0 (DNA-directed RNA polymerase subunit OS=Cucurbita maxima OX=3661 GN=LOC111469417 PE=3 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.4e-75
Identity = 144/146 (98.63%), Postives = 144/146 (98.63%), Query Frame = 0


Query: 1    MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
            MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT
Sbjct: 1255 MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 1314

Query: 61   FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
            FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL
Sbjct: 1315 FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 1374

Query: 121  GTGMLKVRQRVSVPQQLPYGPPAILS 147
            GTGMLKVRQR SVP QLPYGPPAILS
Sbjct: 1375 GTGMLKVRQRASVPHQLPYGPPAILS 1400

[Close]

BLAST of Chy12G214890 vs. ExPASy TrEMBL
Match: A0A6J1HLT9 (DNA-directed RNA polymerase subunit OS=Cucurbita moschata OX=3662 GN=LOC111464789 PE=3 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.4e-75
Identity = 144/146 (98.63%), Postives = 144/146 (98.63%), Query Frame = 0


Query: 1    MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
            MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT
Sbjct: 1255 MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 1314

Query: 61   FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
            FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL
Sbjct: 1315 FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 1374

Query: 121  GTGMLKVRQRVSVPQQLPYGPPAILS 147
            GTGMLKVRQR SVP QLPYGPPAILS
Sbjct: 1375 GTGMLKVRQRASVPHQLPYGPPAILS 1400

[Close]

BLAST of Chy12G214890 vs. NCBI nr
Match: KAA0036972.1 (DNA-directed RNA polymerase [Cucumis melo var. makuwa] >TYK21234.1 DNA-directed RNA polymerase [Cucumis melo var. makuwa])

HSP 1 Score: 291 bits (744), Expect = 2.18e-97
Identity = 145/146 (99.32%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 1   MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
           MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT
Sbjct: 114 MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 173

Query: 61  FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
           FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL
Sbjct: 174 FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 233

Query: 121 GTGMLKVRQRVSVPQQLPYGPPAILS 146
           GTGMLKVRQRVSVP+QLPYGPPAILS
Sbjct: 234 GTGMLKVRQRVSVPRQLPYGPPAILS 259

[Close]

BLAST of Chy12G214890 vs. NCBI nr
Match: XP_031736287.1 (DNA-directed RNA polymerase III subunit 1 isoform X2 [Cucumis sativus])

HSP 1 Score: 292 bits (748), Expect = 3.37e-89
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 1    MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
            MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT
Sbjct: 1070 MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 1129

Query: 61   FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
            FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL
Sbjct: 1130 FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 1189

Query: 121  GTGMLKVRQRVSVPQQLPYGPPAILS 146
            GTGMLKVRQRVSVPQQLPYGPPAILS
Sbjct: 1190 GTGMLKVRQRVSVPQQLPYGPPAILS 1215

[Close]

BLAST of Chy12G214890 vs. NCBI nr
Match: XP_004148776.1 (DNA-directed RNA polymerase III subunit 1 isoform X1 [Cucumis sativus] >KAE8652732.1 hypothetical protein Csa_013764 [Cucumis sativus])

HSP 1 Score: 292 bits (748), Expect = 1.01e-88
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 1    MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
            MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT
Sbjct: 1255 MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 1314

Query: 61   FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
            FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL
Sbjct: 1315 FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 1374

Query: 121  GTGMLKVRQRVSVPQQLPYGPPAILS 146
            GTGMLKVRQRVSVPQQLPYGPPAILS
Sbjct: 1375 GTGMLKVRQRVSVPQQLPYGPPAILS 1400

[Close]

BLAST of Chy12G214890 vs. NCBI nr
Match: XP_008465290.2 (PREDICTED: LOW QUALITY PROTEIN: DNA-directed RNA polymerase III subunit 1 [Cucumis melo])

HSP 1 Score: 291 bits (744), Expect = 3.59e-88
Identity = 145/146 (99.32%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 1    MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
            MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT
Sbjct: 1255 MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 1314

Query: 61   FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
            FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL
Sbjct: 1315 FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 1374

Query: 121  GTGMLKVRQRVSVPQQLPYGPPAILS 146
            GTGMLKVRQRVSVP+QLPYGPPAILS
Sbjct: 1375 GTGMLKVRQRVSVPRQLPYGPPAILS 1400

[Close]

BLAST of Chy12G214890 vs. NCBI nr
Match: XP_022156695.1 (DNA-directed RNA polymerase III subunit 1 [Momordica charantia])

HSP 1 Score: 284 bits (726), Expect = 1.54e-87
Identity = 142/146 (97.26%), Postives = 143/146 (97.95%), Query Frame = 0


Query: 1   MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
           MGTEGVDGC+TKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT
Sbjct: 774 MGTEGVDGCSTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 833

Query: 61  FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
           FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL
Sbjct: 834 FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 893

Query: 121 GTGMLKVRQRVSVPQQLPYGPPAILS 146
           GTGMLKVRQR SVPQ LPYGP AILS
Sbjct: 894 GTGMLKVRQRASVPQHLPYGPDAILS 919

[Close]

BLAST of Chy12G214890 vs. TAIR 10
Match: AT5G60040.1 (nuclear RNA polymerase C1 )

HSP 1 Score: 194.1 bits (492), Expect = 7.8e-50
Identity = 97/146 (66.44%), Postives = 116/146 (79.45%), Query Frame = 0


Query: 1    MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
            MGT G++G  T SN+++EV + LGIEAAR  II+EI   M +HGMSIDIRHMMLL D+MT
Sbjct: 1231 MGTPGINGRTTTSNNVVEVSKTLGIEAARTTIIDEIGTVMGNHGMSIDIRHMMLLADVMT 1290

Query: 61   FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
            +RGEVLGI R GIQKMDKSVLM ASFE+T DHLF+A+ +G+ D IEGV+ECVIMGIPM L
Sbjct: 1291 YRGEVLGIQRTGIQKMDKSVLMQASFERTGDHLFSAAASGKVDNIEGVTECVIMGIPMKL 1350

Query: 121  GTGMLKVRQRVSVPQQLPYGPPAILS 147
            GTG+LKV QR     +L YGP  I+S
Sbjct: 1351 GTGILKVLQRTDDLPKLKYGPDPIIS 1376

[Close]

BLAST of Chy12G214890 vs. TAIR 10
Match: AT5G60040.2 (nuclear RNA polymerase C1 )

HSP 1 Score: 194.1 bits (492), Expect = 7.8e-50
Identity = 97/146 (66.44%), Postives = 116/146 (79.45%), Query Frame = 0


Query: 1    MGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMT 60
            MGT G++G  T SN+++EV + LGIEAAR  II+EI   M +HGMSIDIRHMMLL D+MT
Sbjct: 1246 MGTPGINGRTTTSNNVVEVSKTLGIEAARTTIIDEIGTVMGNHGMSIDIRHMMLLADVMT 1305

Query: 61   FRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPL 120
            +RGEVLGI R GIQKMDKSVLM ASFE+T DHLF+A+ +G+ D IEGV+ECVIMGIPM L
Sbjct: 1306 YRGEVLGIQRTGIQKMDKSVLMQASFERTGDHLFSAAASGKVDNIEGVTECVIMGIPMKL 1365

Query: 121  GTGMLKVRQRVSVPQQLPYGPPAILS 147
            GTG+LKV QR     +L YGP  I+S
Sbjct: 1366 GTGILKVLQRTDDLPKLKYGPDPIIS 1391

[Close]

BLAST of Chy12G214890 vs. TAIR 10
Match: AT4G35800.1 (RNA polymerase II large subunit )

HSP 1 Score: 105.1 bits (261), Expect = 4.7e-23
Identity = 50/120 (41.67%), Postives = 77/120 (64.17%), Query Frame = 0


Query: 4    EGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMTFRG 63
            E VD   T SNH+IE+ +VLGIEA R+ +++E++  +   G  ++ RH+ +L D MT+RG
Sbjct: 1341 EDVDPKRTTSNHLIEIIEVLGIEAVRRALLDELRVVISFDGSYVNYRHLAILCDTMTYRG 1400

Query: 64   EVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPLGTG 123
             ++ ITR GI + D   LM  SFE+T D L +A+     D + GV+E +++G   P+GTG
Sbjct: 1401 HLMAITRHGINRNDTGPLMRCSFEETVDILLDAAAYAETDCLRGVTENIMLGQLAPIGTG 1460

[Close]

BLAST of Chy12G214890 vs. TAIR 10
Match: AT3G57660.1 (nuclear RNA polymerase A1 )

HSP 1 Score: 80.1 bits (196), Expect = 1.6e-15
Identity = 42/121 (34.71%), Postives = 65/121 (53.72%), Query Frame = 0


Query: 13   SNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMTFRGEVLGITRFG 72
            SN I ++  + G+EAAR+ II EI +  +S+G+S+ IRH+ L+ D MTF G    ++R G
Sbjct: 1550 SNSIHDMLNIFGVEAARETIIREINHVFKSYGISVSIRHLNLIADYMTFSGGYRPMSRMG 1609

Query: 73   IQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPLGTGMLKVRQRVS 132
                  S     +FE     +  A+  G  D +E  S  + +G+P   GTG   + QRV 
Sbjct: 1610 GIAESTSPFCRMTFETATKFIVQAATYGEKDTLETPSARICLGLPALSGTGCFDLMQRVE 1669

Query: 133  V 134
            +
Sbjct: 1670 L 1670

[Close]

BLAST of Chy12G214890 vs. TAIR 10
Match: ATCG00170.1 (DNA-directed RNA polymerase family protein )

HSP 1 Score: 47.8 bits (112), Expect = 9.0e-06
Identity = 27/72 (37.50%), Postives = 44/72 (61.11%), Query Frame = 0


Query: 55   LGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIM 114
            L + + +R  +LGITR  +    +S +  ASF++TA  L  A++ GR D ++G+ E V++
Sbjct: 1256 LEEAICYRAVLLGITRASLN--TQSFISEASFQETARVLAKAALRGRIDWLKGLKENVVL 1315

Query: 115  GIPMPLGTGMLK 127
            G  +P GTG  K
Sbjct: 1316 GGVIPAGTGFNK 1325

The following BLAST results are available for this feature:

BLAST of Chy12G214890 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JXF9	1.1e-48	66.44	DNA-directed RNA polymerase III subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRP...	[more]
Q5ZL98	5.3e-43	61.70	DNA-directed RNA polymerase III subunit RPC1 OS=Gallus gallus OX=9031 GN=POLR3A ...	[more]
A4IF62	6.9e-43	61.70	DNA-directed RNA polymerase III subunit RPC1 OS=Bos taurus OX=9913 GN=POLR3A PE=...	[more]
O14802	9.0e-43	61.70	DNA-directed RNA polymerase III subunit RPC1 OS=Homo sapiens OX=9606 GN=POLR3A P...	[more]
Q86AQ5	2.6e-42	63.85	DNA-directed RNA polymerase III subunit rpc1 OS=Dictyostelium discoideum OX=4468...	[more]

BLAST of Chy12G214890 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LXJ4	2.2e-76	100.00	DNA-directed RNA polymerase OS=Cucumis sativus OX=3659 GN=Csa_1G083760 PE=4 SV=1	[more]
A0A5A7T0K7	6.4e-76	99.32	DNA-directed RNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3CNX9	6.4e-76	99.32	DNA-directed RNA polymerase subunit OS=Cucumis melo OX=3656 GN=LOC103502942 PE=3...	[more]
A0A6J1I0L0	2.4e-75	98.63	DNA-directed RNA polymerase subunit OS=Cucurbita maxima OX=3661 GN=LOC111469417 ...	[more]
A0A6J1HLT9	2.4e-75	98.63	DNA-directed RNA polymerase subunit OS=Cucurbita moschata OX=3662 GN=LOC11146478...	[more]

BLAST of Chy12G214890 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0036972.1	2.18e-97	99.32	DNA-directed RNA polymerase [Cucumis melo var. makuwa] >TYK21234.1 DNA-directed ...	[more]
XP_031736287.1	3.37e-89	100.00	DNA-directed RNA polymerase III subunit 1 isoform X2 [Cucumis sativus]	[more]
XP_004148776.1	1.01e-88	100.00	DNA-directed RNA polymerase III subunit 1 isoform X1 [Cucumis sativus] >KAE86527...	[more]
XP_008465290.2	3.59e-88	99.32	PREDICTED: LOW QUALITY PROTEIN: DNA-directed RNA polymerase III subunit 1 [Cucum...	[more]
XP_022156695.1	1.54e-87	97.26	DNA-directed RNA polymerase III subunit 1 [Momordica charantia]	[more]

BLAST of Chy12G214890 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60040.1	7.8e-50	66.44	nuclear RNA polymerase C1	[more]
AT5G60040.2	7.8e-50	66.44	nuclear RNA polymerase C1	[more]
AT4G35800.1	4.7e-23	41.67	RNA polymerase II large subunit	[more]
AT3G57660.1	1.6e-15	34.71	nuclear RNA polymerase A1	[more]
ATCG00170.1	9.0e-06	37.50	DNA-directed RNA polymerase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007081	RNA polymerase Rpb1, domain 5	PFAM	PF04998	RNA_pol_Rpb1_5	coord: 5..80 e-value: 1.5E-17 score: 64.0
None	No IPR available	GENE3D	1.10.150.390		coord: 78..137 e-value: 1.7E-21 score: 77.5
None	No IPR available	PANTHER	PTHR19376:SF32	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1	coord: 1..131
None	No IPR available	PANTHER	PTHR19376	DNA-DIRECTED RNA POLYMERASE	coord: 1..131
None	No IPR available	SUPERFAMILY	64484	beta and beta-prime subunits of DNA dependent RNA-polymerase	coord: 2..130

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G214890.1	Chy12G214890.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0046872	metal ion binding