Chy12G210340 (gene) Cucumber (hystrix) v1

BLAST of Chy12G210340 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 6.1e-142
Identity = 249/512 (48.63%), Postives = 346/512 (67.58%), Query Frame = 0

Query: 38  DSFVQCLFDYGSPSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDFPKPLAIITPLHV 97
           ++F++CL  Y  P+    A L+YT  +  Y SILN ++QNLRF +   PKPL I TPL+V
Sbjct: 32  ENFLKCLSQY-IPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNV 91

Query: 98  SHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVVVDLINLRSISIDVENQNAWVQ 157
           SHIQ  ++CSK  G QIRTRSGGHD EG+SYIS +PFV+VDL N+ S+ IDV +Q AWV+
Sbjct: 92  SHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVE 151

Query: 158 SGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLV 217
           SGATLGE+YY I+E ++ L+FPAG CPTVG GGH SGGGYG L+R YGLAAD++IDA+LV
Sbjct: 152 SGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLV 211

Query: 218 DANGKVHNKESMGQDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRIL 277
           + +GKV +++SMG+DLFWAIRGGGG +FGI+ AWK++LV VP+  TI    + +E   ++
Sbjct: 212 NVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELV 271

Query: 278 KLIHEWQYVAPKLDANLLL-------------GISLT--------------EEVLTILKP 337
           KL+++WQ +A   +  LLL             G + T              + ++ ++  
Sbjct: 272 KLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNK 331

Query: 338 TFPQLGLTKEDCLEMSWIQSVL----LMGWFQKGDPLEVLLNRSRLYSEISKIKSDYVKE 397
           +FP+LG+ K DC ++SWI +++    ++ +       E+LL+RS        IK DYVK+
Sbjct: 332 SFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKK 391

Query: 398 HIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFPHRSDYSYLVGYIAKWK 457
            IP  A+  + E+L  +DV +   +F PYGG M E+ +S  PFPHR+  +Y + YIA W 
Sbjct: 392 PIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASW- 451

Query: 458 NESVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRASYKRAKVWGL 517
            E  E  + H+NWIR +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y +A++WG 
Sbjct: 452 -EKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGE 511

Query: 518 KYFGNNFDRLVYVKTKVDPSDFFRHEQSIPNL 519
           KYFG NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Chy12G210340 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 8.0e-142
Identity = 250/524 (47.71%), Postives = 351/524 (66.98%), Query Frame = 0

Query: 13  LIAFIIIVVVVLSTATTSFCSTQESDSFVQCLFDYGSPSTNPIANLIYTPTNSSYSSILN 72
           ++ F     ++LS       +     SF+QCL       +N ++ +I+TP ++S+SS+L 
Sbjct: 10  VLIFFTTSTLLLSIPHPVSANRSNQSSFLQCL-SLQLNDSNIVSKVIHTPNDTSFSSVLA 69

Query: 73  FSIQNLRFLNKDFPKPLAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDI 132
            SIQN RF   D PKP+ I+TP+  S +Q+AV C++  G  IRTRSGGHD+EGLSY++  
Sbjct: 70  SSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHK 129

Query: 133 PFVVVDLINLRSISIDVENQNAWVQSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHL 192
           PFV++DL NLRSI++DV+N++ WVQ+GAT+GELYY I +K++TLAFPAG CPTVGVGGH 
Sbjct: 130 PFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHF 189

Query: 193 SGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGQDLFWAIRGGGGGSFGIVVAWK 252
           SGGGYG L+RK+GLAADHVIDA +VDA G++  +  MG+D FWAIRGGGG SF +V++WK
Sbjct: 190 SGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWK 249

Query: 253 VKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL------------ 312
           + L+ VP+ VT+    +   E   LK+IH WQ+VA K+  +L + + L            
Sbjct: 250 IGLINVPSTVTVFNVTK-FSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYKNMVRASFP 309

Query: 313 ------TEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQK--GDPLEVLLNRSRL 372
                  + +L ++   FP+LGL ++DC EMSWI+SV+   WF +   +P+ VL  R+R 
Sbjct: 310 GLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVI---WFAELGEEPINVLTKRTRA 369

Query: 373 YSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFPHR 432
            S   K KSD+V+E +P  A+  +W RL+  + E +Q++F P+GGKMSE+ D ETPFPHR
Sbjct: 370 -SLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHR 429

Query: 433 SDYSYLVGYIAKWKNESVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNK 492
               Y + Y+  W+    + ++ ++ W+  +YD M+ FV+KSPR AY+N RDLD+G    
Sbjct: 430 KGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVG 489

Query: 493 YGRASYKRAKVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIP 517
             R+ Y+  K WG+KYF NNF+RLV VKT VDPSDFF  EQSIP
Sbjct: 490 VKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Chy12G210340 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 1.4e-141
Identity = 251/516 (48.64%), Postives = 346/516 (67.05%), Query Frame = 0

Query: 38  DSFVQCLFDYGSPSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDFPKPLAIITPLHV 97
           ++F++C   Y  P+    A L+YT  +  Y SILN +IQNLRF +   PKPL IITPL+V
Sbjct: 32  ENFLKCFSQY-IPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNV 91

Query: 98  SHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVVVDLINLRSISIDVENQNAWVQ 157
           SHIQ  ++CSK  G QIRTRSGGHD EG+SYIS +PFV+VDL N+ S+ IDV +Q AWV+
Sbjct: 92  SHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVE 151

Query: 158 SGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLV 217
           +GATLGE+YY I+E ++ L+FPAG CPTVG GGH SGGGYG L+R YGLAAD++IDA+LV
Sbjct: 152 AGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLV 211

Query: 218 DANGKVHNKESMGQDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRIL 277
           + +GKV +++SMG+DLFWAIRGGGG +FGI+ AWK++LV VP+  TI    + +E   ++
Sbjct: 212 NVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELV 271

Query: 278 KLIHEWQYVAPKLDANLLL-------------GISLT--------------EEVLTILKP 337
           KL+++WQ +A   +  LLL             G + T              + ++ ++  
Sbjct: 272 KLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNK 331

Query: 338 TFPQLGLTKEDCLEMSWIQSVLLMG--------WFQKGDPLEVLLNRSRLYSEISKIKSD 397
           +FP+LG+ K DC ++SWI +++           +F+K    E+LL+RS        IK D
Sbjct: 332 SFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKK----EILLDRSGGRKAAFSIKLD 391

Query: 398 YVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFPHRSDYSYLVGYI 457
           YVK+ IP  A+  + E+L  +DV +   +F PYGG M E+ +S  PFPHR+   Y + YI
Sbjct: 392 YVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYI 451

Query: 458 AKWKNESVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRASYKRAK 517
           A W  E  E  + H+NWIR +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y +A+
Sbjct: 452 ASW--EKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQAR 511

Query: 518 VWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPNL 519
           +WG KYFG NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 512 IWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Chy12G210340 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 7.5e-140
Identity = 249/512 (48.63%), Postives = 343/512 (66.99%), Query Frame = 0

Query: 38  DSFVQCLFDYGSPSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDFPKPLAIITPLHV 97
           ++F++C F    P+      L+YT  +  Y SILN +IQNLRF++   PKPL I+TP + 
Sbjct: 32  ENFLKC-FSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNN 91

Query: 98  SHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVVVDLINLRSISIDVENQNAWVQ 157
           SHIQA ++CSK  G QIRTRSGGHD EG+SYIS +PFVVVDL N+ SI IDV +Q AWV+
Sbjct: 92  SHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVE 151

Query: 158 SGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLV 217
           +GATLGE+YY I+EK++ L+FP G CPTVGVGGH SGGGYG L+R YGLAAD++IDA+LV
Sbjct: 152 AGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLV 211

Query: 218 DANGKVHNKESMGQDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRIL 277
           + +GKV +++SMG+DLFWAIRGGGG +FGI+ AWK+KLV VP++ TI    + +E   ++
Sbjct: 212 NVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLV 271

Query: 278 KLIHEWQYVAPKLDANLLL-------------GISLT--------------EEVLTILKP 337
           KL ++WQ +A K D +L+L             G + T              + ++ ++  
Sbjct: 272 KLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNK 331

Query: 338 TFPQLGLTKEDCLEMSWIQSVL----LMGWFQKGDPLEVLLNRSRLYSEISKIKSDYVKE 397
           +FP+LG+ K DC E SWI + +    ++ +       E+LL+RS        IK DYVK+
Sbjct: 332 SFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKK 391

Query: 398 HIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFPHRSDYSYLVGYIAKWK 457
            IP  A+  + E+L  +DV     +  PYGG M E+ +S  PFPHR+   Y + Y A W 
Sbjct: 392 PIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASW- 451

Query: 458 NESVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRASYKRAKVWGL 517
            E  E  + H+NW+R +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y +A++WG 
Sbjct: 452 -EKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGE 511

Query: 518 KYFGNNFDRLVYVKTKVDPSDFFRHEQSIPNL 519
           KYFG NF+RLV VKTKVDP++FFR+EQSIP L
Sbjct: 512 KYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of Chy12G210340 vs. ExPASy Swiss-Prot
Match: Q9FKV0 (Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=2 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 1.8e-138
Identity = 258/539 (47.87%), Postives = 357/539 (66.23%), Query Frame = 0

Query: 10  PFGLIAFIIIVVVVLS----TATTSFCSTQESDSFVQCLFDYGSPSTNPIANLIYTPTN- 69
           P   I+ II+ V+  S    T T+S  S Q  D F+ C+    +  + P+   ++TP   
Sbjct: 6   PLPTISCIIVSVLYFSFYCITPTSSSASIQ--DQFINCV-KRNTHVSFPLEKTLFTPAKN 65

Query: 70  -SSYSSILNFSIQNLRFLNKDFPKPLAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDF 129
            S ++ +L  + QNL+FL K  PKP  I  P+H S +QA++ICSK  G   R RSGGHDF
Sbjct: 66  VSLFNQVLESTAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDF 125

Query: 130 EGLSYISDI--PFVVVDLINLRSISIDVENQNAWVQSGATLGELYYRISEKSQTLAFPAG 189
           E LSY+S I  PF+++DL  L+ I++D+E+ +AWVQ GATLGELYYRI+EKS+   FPAG
Sbjct: 126 EALSYVSRIEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAG 185

Query: 190 SCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGQDLFWAIRGGG 249
            C +VG+GG+++GGGYG L+RKYGLA D+V+D  +VDANGK+ ++ +MG+DLFWAIRGGG
Sbjct: 186 LCTSVGIGGYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGG 245

Query: 250 GGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKL----------- 309
           G SFGIV+AWK+KLV VP  VT+    +TLE+D  LK I +WQ ++ K+           
Sbjct: 246 GASFGIVLAWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLR 305

Query: 310 ----DAN-----LLLGISLTEE--VLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQK 369
               D N       LG  L E+  +L +++  FP+LGLT++DC EMSWI++ L  G F  
Sbjct: 306 AAGNDGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPT 365

Query: 370 GDPLEVLLN-RSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGK 429
           G P+E+LL  +S L  +  K  SD+VKE IP++ +KG+++RL   +     + + PYGG 
Sbjct: 366 GSPIEILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTF--LNWTPYGGM 425

Query: 430 MSEVDDSETPFPHRSDYSYLVGYIAKW-KNESVEAEKSHLNWIREIYDYMTPFVSKSPRA 489
           MS++ +S  PFPHR+   + + Y A W +N+   + K  +NWI+EIY+YM P+VS +PR 
Sbjct: 426 MSKIPESAIPFPHRNGTLFKILYYANWLENDKTSSRK--INWIKEIYNYMAPYVSSNPRQ 485

Query: 490 AYVNYRDLDIGTNNKYGRASYKRAKVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIP 517
           AYVNYRDLD G N    + ++  AK+WG KYF  NFDRLV +KTKVDP +FFRHEQSIP
Sbjct: 486 AYVNYRDLDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIP 537

BLAST of Chy12G210340 vs. ExPASy TrEMBL
Match: A0A5D3C112 (Cannabidiolic acid synthase-like 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00400 PE=3 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 5.7e-284
Identity = 493/556 (88.67%), Postives = 511/556 (91.91%), Query Frame = 0

Query: 1   MKCFPILPAPFGLIAFIIIVVVVLSTATTSFCSTQESDSFVQCLFDYGSPSTNPIANLIY 60
           MKCFPIL APFGLIAFII  VVVLSTATTSFC TQE DSFVQCLFDYGSP++NPIAN+IY
Sbjct: 1   MKCFPILAAPFGLIAFII--VVVLSTATTSFC-TQECDSFVQCLFDYGSPNSNPIANVIY 60

Query: 61  TPTNSSYSSILNFSIQNLRFLNKDFPKPLAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120
           TP+NSSYSSILNFSIQNLRFLNK+  KPLAIITP HVSHIQAAVICSKSHGFQIRTRSGG
Sbjct: 61  TPSNSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGG 120

Query: 121 HDFEGLSYISDIPFVVVDLINLRSISIDVENQNAWVQSGATLGELYYRISEKSQTLAFPA 180
           HDFEGLSYIS +PFV+VDLINL SISIDVENQNAWV+SGATLGELYYRI+EKSQTLAFPA
Sbjct: 121 HDFEGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGQDLFWAIRGG 240
           GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMG+DLFWAIRGG
Sbjct: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240

Query: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300
           GGG FGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL
Sbjct: 241 GGGCFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300

Query: 301 T--------------------------EEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLM 360
           T                          EEVLTILKPTFPQL LTKEDCLEMSWIQSVLLM
Sbjct: 301 TGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLM 360

Query: 361 GWFQKGDPLEVLLNRSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVP 420
           GWFQKGDPLEVLLNR+RLYSEISKIKSDYVKEHIP+VA+KGMWERLKS+DVELSQIMFVP
Sbjct: 361 GWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVP 420

Query: 421 YGGKMSEVDDSETPFPHRSDYSYLVGYIAKWKNESVEAEKSHLNWIREIYDYMTPFVSKS 480
           YGGKMSEVDDSETPF HRSDYSYLVGYIAKWKN SVEAE+ HLNWIREIYDYMTPFVSKS
Sbjct: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKS 480

Query: 481 PRAAYVNYRDLDIGTNNKYGRASYKRAKVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQS 531
           PRAAYVNYRDLDIGTNNKYGR SYKRA+VWGLKYFG NFD+LVYVKTKVDPSDFFRH+QS
Sbjct: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQS 540

BLAST of Chy12G210340 vs. ExPASy TrEMBL
Match: A0A1S3BEA9 (cannabidiolic acid synthase-like 2 OS=Cucumis melo OX=3656 GN=LOC103488685 PE=3 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 5.7e-284
Identity = 493/556 (88.67%), Postives = 511/556 (91.91%), Query Frame = 0

Query: 1   MKCFPILPAPFGLIAFIIIVVVVLSTATTSFCSTQESDSFVQCLFDYGSPSTNPIANLIY 60
           MKCFPIL APFGLIAFII  VVVLSTATTSFC TQE DSFVQCLFDYGSP++NPIAN+IY
Sbjct: 1   MKCFPILAAPFGLIAFII--VVVLSTATTSFC-TQECDSFVQCLFDYGSPNSNPIANVIY 60

Query: 61  TPTNSSYSSILNFSIQNLRFLNKDFPKPLAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120
           TP+NSSYSSILNFSIQNLRFLNK+  KPLAIITP HVSHIQAAVICSKSHGFQIRTRSGG
Sbjct: 61  TPSNSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGG 120

Query: 121 HDFEGLSYISDIPFVVVDLINLRSISIDVENQNAWVQSGATLGELYYRISEKSQTLAFPA 180
           HDFEGLSYIS +PFV+VDLINL SISIDVENQNAWV+SGATLGELYYRI+EKSQTLAFPA
Sbjct: 121 HDFEGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGQDLFWAIRGG 240
           GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMG+DLFWAIRGG
Sbjct: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240

Query: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300
           GGG FGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL
Sbjct: 241 GGGCFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300

Query: 301 T--------------------------EEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLM 360
           T                          EEVLTILKPTFPQL LTKEDCLEMSWIQSVLLM
Sbjct: 301 TGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLM 360

Query: 361 GWFQKGDPLEVLLNRSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVP 420
           GWFQKGDPLEVLLNR+RLYSEISKIKSDYVKEHIP+VA+KGMWERLKS+DVELSQIMFVP
Sbjct: 361 GWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVP 420

Query: 421 YGGKMSEVDDSETPFPHRSDYSYLVGYIAKWKNESVEAEKSHLNWIREIYDYMTPFVSKS 480
           YGGKMSEVDDSETPF HRSDYSYLVGYIAKWKN SVEAE+ HLNWIREIYDYMTPFVSKS
Sbjct: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKS 480

Query: 481 PRAAYVNYRDLDIGTNNKYGRASYKRAKVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQS 531
           PRAAYVNYRDLDIGTNNKYGR SYKRA+VWGLKYFG NFD+LVYVKTKVDPSDFFRH+QS
Sbjct: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQS 540

BLAST of Chy12G210340 vs. ExPASy TrEMBL
Match: A0A1S3BE36 (cannabidiolic acid synthase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488617 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 1.2e-201
Identity = 344/536 (64.18%), Postives = 420/536 (78.36%), Query Frame = 0

Query: 11  FGLIAFIIIVVVVLSTATTSFCSTQESDSFVQCLFDYGSPSTNPIANLIYTPTNSSYSSI 70
           F L  F +  +V+   + +   ST++ ++F+QCLF+    +   I+NLIYTP NSS+ S+
Sbjct: 6   FLLTPFALFFIVLTFLSPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPINSSFYSV 65

Query: 71  LNFSIQNLRFLNKDFPKPLAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYIS 130
           LNFSIQNLRF  K  PKPLAIITP HVSHIQAA+ICSKSH  QIR RSGGHDFEGLSY+S
Sbjct: 66  LNFSIQNLRFSRKQTPKPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDFEGLSYVS 125

Query: 131 DIPFVVVDLINLRSISIDVENQNAWVQSGATLGELYYRISEKSQTLAFPAGSCPTVGVGG 190
           D+PF+VVDLINLRSI+IDVE +NAWVQSGATLGE YYRI EKS+TLAFPAGSCPTVG+GG
Sbjct: 126 DVPFIVVDLINLRSITIDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSCPTVGIGG 185

Query: 191 HLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGQDLFWAIRGGGGGSFGIVVA 250
           HLSGGG+GWL+RKYGLAAD+VIDA  +DANGKV+++ESMG+DLFWAIRGGGGGSFGI+VA
Sbjct: 186 HLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGIIVA 245

Query: 251 WKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLT--------- 310
           WKVKLV VPA VTIC + R+LEE+  +KLIH+WQY+   LD NLLLGISLT         
Sbjct: 246 WKVKLVRVPATVTICGSKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGGNLTQESG 305

Query: 311 ----------------EEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKGDPLEV 370
                            E++ IL   FP+L L+KE+C EMSWI++VL+M  F   +P EV
Sbjct: 306 KTNPNALFSSFFLGKVNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGFPTHEPFEV 365

Query: 371 LLNRSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDS 430
           LLNR+ L+   +KIKSDY+K+ +   A K M +RLK++D+E++QIMF+PYGG+MSE+  S
Sbjct: 366 LLNRTPLFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGGRMSEISKS 425

Query: 431 ETPFPHRSDYSYLVGYIAKWKNESVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDL 490
           ETPFPHR    Y +GY  KWK++S++AEK HL+WIR+IYDYMTPFVSKSPRA Y NYRDL
Sbjct: 426 ETPFPHRVGNIYKLGYYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRATYSNYRDL 485

Query: 491 DIGTNNKYGRASYKRAKVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPNLSGI 522
           DIG NNKYG+A+Y  A+VWG KYFG NFDRLV+VKT +DP+DFFR+EQSIP L  I
Sbjct: 486 DIGINNKYGKATYSHARVWGFKYFGKNFDRLVHVKTMIDPNDFFRNEQSIPALIDI 541

BLAST of Chy12G210340 vs. ExPASy TrEMBL
Match: A0A6J1DEU9 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020168 PE=3 SV=1)

HSP 1 Score: 712.2 bits (1837), Expect = 1.6e-201
Identity = 351/545 (64.40%), Postives = 419/545 (76.88%), Query Frame = 0

Query: 1   MKCFPILPAPFGLIAFIIIVVVVLSTATTSFCSTQESDSFVQCLFDYGSPSTNPIANLIY 60
           MK FP+ P     +AF     +VLS++     S  + + F+QCL  + SPS N I+  IY
Sbjct: 1   MKSFPLNP-----LAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRH-SPSNNSISKFIY 60

Query: 61  TPTNSSYSSILNFSIQNLRFLNKDFPKPLAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120
           TP N SYSSILNFSIQNLRF   + PKPL +I P H+SHIQAAVICSK+HG QIRTRSGG
Sbjct: 61  TPANFSYSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGG 120

Query: 121 HDFEGLSYISDIPFVVVDLINLRSISIDVENQNAWVQSGATLGELYYRISEKSQTLAFPA 180
           HDFEGLSY+S +PF+V+DLI+LRSI+ID +N  AWVQSGATLGELYYRI++KSQTLAFPA
Sbjct: 121 HDFEGLSYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGQDLFWAIRGG 240
           GSCPTVGVGGHLSGGGYG+LIRKYGLAADHVIDA+LVDANG++ N+ESM  DLFW IRGG
Sbjct: 181 GSCPTVGVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGG 240

Query: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300
           GGGSFGIVVAWK++LV VP  +T C  NRTL+ D + KLIH WQYVA KL+ NLLLGISL
Sbjct: 241 GGGSFGIVVAWKLRLVPVPTTLTTCTVNRTLDRDGV-KLIHRWQYVAHKLEDNLLLGISL 300

Query: 301 T--------------------------EEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLM 360
           T                          +E++ I+  TFP+LGLTKEDC+E SWI+S L+ 
Sbjct: 301 TGGHILTQQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLIT 360

Query: 361 GWFQKGDPLEVLLNRSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVP 420
             F  G+ LE LL R  L +  +KIKSDYVKE I     KG+WERL +QD+EL+Q+MFVP
Sbjct: 361 AGFSAGESLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVP 420

Query: 421 YGGKMSEVDDSETPFPHRSDYSYLVGYIAKWKNESVEAEKSHLNWIREIYDYMTPFVSKS 480
           YGG+MSE  +SETP+PHR++  Y +GYI KW+ +S +AEK HLNW+REIYDYM P+V+KS
Sbjct: 421 YGGRMSEFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKS 480

Query: 481 PRAAYVNYRDLDIGTNNKYGRASYKRAKVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQS 520
           PRAAYVNYRDLDIG NN+Y + +Y+RAKVWGLKYFG NF+RLV VKT+VDPSDFFRHEQS
Sbjct: 481 PRAAYVNYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQS 538

BLAST of Chy12G210340 vs. ExPASy TrEMBL
Match: A0A5A7TU57 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00190 PE=3 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 1.5e-196
Identity = 338/532 (63.53%), Postives = 416/532 (78.20%), Query Frame = 0

Query: 13  LIAFIIIVVVVLSTATTSFCSTQESDSFVQCLFDYGSPSTNPIANLIYTPTNSSYSSILN 72
           LI   ++ +VV S +  +  S Q+  +F+QCL ++ S S  PI+ ++YTP NSSYSS+L+
Sbjct: 7   LIPLSLVFIVVSSASLCASASPQKYQNFLQCLSEHSSKS-YPISKVVYTPINSSYSSVLD 66

Query: 73  FSIQNLRFLNKDFPKPLAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDI 132
           FSI+NL F   + PKPL IITP HVSHIQAAVICSKSHG QIRTRSGGHDFEGLSY++  
Sbjct: 67  FSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYR 126

Query: 133 PFVVVDLINLRSISIDVENQNAWVQSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHL 192
           PF+VVDLINLRS+ ++VEN  AWV+SGATLGELYYRI EKS+TLAFPAG CPTVG+GGH 
Sbjct: 127 PFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHF 186

Query: 193 SGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGQDLFWAIRGGGGGSFGIVVAWK 252
           SGGGYG ++RK+GLAAD+VIDAYLVDANGKV +KESMG+DLFWAIRGGGGGSFGIVVAWK
Sbjct: 187 SGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGIVVAWK 246

Query: 253 VKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLT----------- 312
           +KLV VP  VTIC  +R LEED I KLIH WQYV  KLD N+ LGI LT           
Sbjct: 247 IKLVPVPETVTICSTDRNLEEDAI-KLIHRWQYVGNKLDENIYLGIILTGGNASTQAGIT 306

Query: 313 --------------EEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMG-WFQKGDPLEVL 372
                         +E++T L   FP+LGL K+DC+E SW++S L++    Q  + LE L
Sbjct: 307 NPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIETLEPL 366

Query: 373 LNRSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSE 432
           LNR+    + +KIKSDY+ E IP+ A+K +W+RLK+QD+E SQ++FVPYGG+MS++ +SE
Sbjct: 367 LNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQISESE 426

Query: 433 TPFPHRSDYSYLVGYIAKWKNESVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLD 492
           TPF HR+ Y + +GY+  WK++S++A+K H++W+RE+Y+YM PFVSKSPRAAY NYRDLD
Sbjct: 427 TPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTNYRDLD 486

Query: 493 IGTNNKYGRASYKRAKVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPNL 519
           IG+NNKYG+ SYKRA VWGLKYFGNNFDRLVYVKTKVDP DFFRHEQSIP L
Sbjct: 487 IGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Chy12G210340 vs. NCBI nr
Match: XP_004135742.2 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653322.1 hypothetical protein Csa_023372 [Cucumis sativus])

HSP 1 Score: 1016 bits (2627), Expect = 0.0
Identity = 511/556 (91.91%), Postives = 519/556 (93.35%), Query Frame = 0

Query: 1   MKCFPILPAPFGLIAFIIIVVVVLSTATTSFCSTQESDSFVQCLFDYGSPSTNPIANLIY 60
           MKCFPILPAPFGLIAFIII+VVVLSTAT SFCSTQES+SFVQCLFDYGS STNPIANLIY
Sbjct: 1   MKCFPILPAPFGLIAFIIIIVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIY 60

Query: 61  TPTNSSYSSILNFSIQNLRFLNKDFPKPLAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120
           TPTNSSYSSILNFSIQNLRFLNKD PKP AIITPLHVSHIQAAVICSKSHGFQIRTRSGG
Sbjct: 61  TPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120

Query: 121 HDFEGLSYISDIPFVVVDLINLRSISIDVENQNAWVQSGATLGELYYRISEKSQTLAFPA 180
           HDFEGLSYISDIPFV+VDLINL+SISIDVENQNAWV SGATLGELYYRISEKSQTLAFPA
Sbjct: 121 HDFEGLSYISDIPFVLVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGQDLFWAIRGG 240
           GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMG+DLFWAIRGG
Sbjct: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240

Query: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300
           GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL
Sbjct: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300

Query: 301 T--------------------------EEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLM 360
           T                          EEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLM
Sbjct: 301 TGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLM 360

Query: 361 GWFQKGDPLEVLLNRSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVP 420
           GWFQK DPLEVLLNRSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVP
Sbjct: 361 GWFQKEDPLEVLLNRSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVP 420

Query: 421 YGGKMSEVDDSETPFPHRSDYSYLVGYIAKWKNESVEAEKSHLNWIREIYDYMTPFVSKS 480
           YGGKMSEVDDSETPF HRSDYSYLVGYIAKWKN SVEAEKSHLNWIREIYDYMTPFVSKS
Sbjct: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKS 480

Query: 481 PRAAYVNYRDLDIGTNNKYGRASYKRAKVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQS 530
           PRAAYVNYRDLDIGTNNKYGR SYKRA+VWGLKYFG NFDRLV+VKTKVDPSDFFRHEQS
Sbjct: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS 540

BLAST of Chy12G210340 vs. NCBI nr
Match: XP_008445757.1 (PREDICTED: cannabidiolic acid synthase-like 2 [Cucumis melo] >KAA0047085.1 cannabidiolic acid synthase-like 2 [Cucumis melo var. makuwa] >TYK05070.1 cannabidiolic acid synthase-like 2 [Cucumis melo var. makuwa])

HSP 1 Score: 979 bits (2530), Expect = 0.0
Identity = 493/556 (88.67%), Postives = 511/556 (91.91%), Query Frame = 0

Query: 1   MKCFPILPAPFGLIAFIIIVVVVLSTATTSFCSTQESDSFVQCLFDYGSPSTNPIANLIY 60
           MKCFPIL APFGLIAFII  VVVLSTATTSFC TQE DSFVQCLFDYGSP++NPIAN+IY
Sbjct: 1   MKCFPILAAPFGLIAFII--VVVLSTATTSFC-TQECDSFVQCLFDYGSPNSNPIANVIY 60

Query: 61  TPTNSSYSSILNFSIQNLRFLNKDFPKPLAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120
           TP+NSSYSSILNFSIQNLRFLNK+  KPLAIITP HVSHIQAAVICSKSHGFQIRTRSGG
Sbjct: 61  TPSNSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGG 120

Query: 121 HDFEGLSYISDIPFVVVDLINLRSISIDVENQNAWVQSGATLGELYYRISEKSQTLAFPA 180
           HDFEGLSYIS +PFV+VDLINL SISIDVENQNAWV+SGATLGELYYRI+EKSQTLAFPA
Sbjct: 121 HDFEGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGQDLFWAIRGG 240
           GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMG+DLFWAIRGG
Sbjct: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240

Query: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300
           GGG FGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL
Sbjct: 241 GGGCFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300

Query: 301 T--------------------------EEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLM 360
           T                          EEVLTILKPTFPQL LTKEDCLEMSWIQSVLLM
Sbjct: 301 TGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLM 360

Query: 361 GWFQKGDPLEVLLNRSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVP 420
           GWFQKGDPLEVLLNR+RLYSEISKIKSDYVKEHIP+VA+KGMWERLKS+DVELSQIMFVP
Sbjct: 361 GWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVP 420

Query: 421 YGGKMSEVDDSETPFPHRSDYSYLVGYIAKWKNESVEAEKSHLNWIREIYDYMTPFVSKS 480
           YGGKMSEVDDSETPF HRSDYSYLVGYIAKWKN SVEAE+ HLNWIREIYDYMTPFVSKS
Sbjct: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKS 480

Query: 481 PRAAYVNYRDLDIGTNNKYGRASYKRAKVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQS 530
           PRAAYVNYRDLDIGTNNKYGR SYKRA+VWGLKYFG NFD+LVYVKTKVDPSDFFRH+QS
Sbjct: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQS 540

BLAST of Chy12G210340 vs. NCBI nr
Match: XP_038895846.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 889 bits (2297), Expect = 0.0
Identity = 442/554 (79.78%), Postives = 487/554 (87.91%), Query Frame = 0

Query: 1   MKCFPILPAPFGLIAFIIIVVVVLSTATTSFCSTQESDSFVQCLFDYGSPSTNPIANLIY 60
           M+CFP L AP G IAFII +V  L+T TTSFC TQESDSFVQCLFDYGS +T+PI+++IY
Sbjct: 1   MECFP-LTAPLGRIAFIIFIV--LTTTTTSFC-TQESDSFVQCLFDYGSLNTHPISDVIY 60

Query: 61  TPTNSSYSSILNFSIQNLRFLNKDFPKPLAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120
           TP NSSYSS+LNFS+QNLRFL K  PKPLAIITP HVSHIQAAVICSKSHG QIRTRSGG
Sbjct: 61  TPINSSYSSVLNFSVQNLRFLTKKTPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGG 120

Query: 121 HDFEGLSYISDIPFVVVDLINLRSISIDVENQNAWVQSGATLGELYYRISEKSQTLAFPA 180
           HDFEGLSYISD PF+VVDLINLRSI+IDVEN+NAWVQSGATLGELYYRI+EKSQTLAFPA
Sbjct: 121 HDFEGLSYISDAPFIVVDLINLRSIAIDVENENAWVQSGATLGELYYRIAEKSQTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGQDLFWAIRGG 240
           GSC TVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGK+H++ESMG+DLFWAIRGG
Sbjct: 181 GSCSTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKLHDRESMGEDLFWAIRGG 240

Query: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300
           GGGSFGIVV+WKVKLV VPA VT+CEANRTLEED ++KLIHEWQYVAPKLDANLLLGISL
Sbjct: 241 GGGSFGIVVSWKVKLVRVPATVTVCEANRTLEED-VMKLIHEWQYVAPKLDANLLLGISL 300

Query: 301 T--------------------------EEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLM 360
           T                          EE++TILKPTF QLGL +EDCLE+SWI+SVLLM
Sbjct: 301 TGGKVTEDEEGKINPIASFVAQFLGKAEELMTILKPTFSQLGLKREDCLEISWIESVLLM 360

Query: 361 GWFQKGDPLEVLLNRSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVP 420
           GWFQK DP+EVLLNR+RLYSEISKIKSDYVKE IP  ++KGMWERLKSQDVEL+QIMFVP
Sbjct: 361 GWFQKEDPIEVLLNRTRLYSEISKIKSDYVKERIPKASIKGMWERLKSQDVELAQIMFVP 420

Query: 421 YGGKMSEVDDSETPFPHRSDYSYLVGYIAKWKNESVEAEKSHLNWIREIYDYMTPFVSKS 480
           YGG+MSEV + ETP+ HR DY YL+GYIAKWKN +VEAEK HLNWIREIYDYMTPFVS S
Sbjct: 421 YGGRMSEVYEWETPYSHRGDYMYLIGYIAKWKNGTVEAEKRHLNWIREIYDYMTPFVSNS 480

Query: 481 PRAAYVNYRDLDIGTNNKYGRASYKRAKVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQS 528
           PR AYVNYRDLDIGTNNKY + SYKRA+ WG+KYFGNNFDRLVY+KTKVDPS+FF+HEQS
Sbjct: 481 PRVAYVNYRDLDIGTNNKYDKTSYKRARNWGMKYFGNNFDRLVYIKTKVDPSNFFKHEQS 540

BLAST of Chy12G210340 vs. NCBI nr
Match: XP_038895208.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 730 bits (1884), Expect = 7.54e-260
Identity = 361/514 (70.23%), Postives = 420/514 (81.71%), Query Frame = 0

Query: 33  STQESDSFVQCLFDYGSPSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDFPKPLAII 92
           ST++ ++FVQCL +  S ST PI+NLIYTP NSSYSSILNFSIQNLRF +K+ PKPLAII
Sbjct: 26  STKKHENFVQCLLN-NSLSTYPISNLIYTPINSSYSSILNFSIQNLRFSSKETPKPLAII 85

Query: 93  TPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVVVDLINLRSISIDVENQ 152
           TP HVSHIQAA+ICSKSH  QIRTRSGGHDFEGLSYISD+PFVVVDLINL+SI+IDVEN+
Sbjct: 86  TPSHVSHIQAAIICSKSHALQIRTRSGGHDFEGLSYISDVPFVVVDLINLKSIAIDVENE 145

Query: 153 NAWVQSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVI 212
            AWVQSGATLGELYYRI+EKS+TLAFPAGSCPTVGVGGHLSGGG+GWL+RKYGLAAD+VI
Sbjct: 146 TAWVQSGATLGELYYRIAEKSRTLAFPAGSCPTVGVGGHLSGGGFGWLMRKYGLAADNVI 205

Query: 213 DAYLVDANGKVHNKESMGQDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLE 272
           DA  +DANGKV+++ESMG+DLFWAIRGGGGGSFGIVVAWKVKLV VPA VTIC  NR LE
Sbjct: 206 DASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPATVTICGINRNLE 265

Query: 273 EDRILKLIHEWQYVAPKLDANLLLGISL-------------------------TEEVLTI 332
           E+  +KLIH WQYV  KLD NLLLGISL                           E++TI
Sbjct: 266 EEDTIKLIHRWQYVTNKLDENLLLGISLIGGNSTQEGGKTNPMALFSSFFLGKVNELVTI 325

Query: 333 LKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKGDPLEVLLNRSRLYSEISKIKSDYVKEH 392
           L   F +LGL KEDC EMSWI+SVL+M  F K + LEVLLNR+      +KIKSDYVKE 
Sbjct: 326 LNTNFSELGLAKEDCSEMSWIKSVLIMAGFPKQEALEVLLNRAPPSGLSTKIKSDYVKEP 385

Query: 393 IPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFPHRSDYSYLVGYIAKWKN 452
           I   + K M++RLK++D+E++QIMF+PYGG+MSE+ +S TPFPHR+   Y +GY  KWK 
Sbjct: 386 ISKASFKTMFKRLKAEDIEVAQIMFIPYGGRMSEISESLTPFPHRAGNIYKLGYYVKWKE 445

Query: 453 ESVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRASYKRAKVWGLK 512
           +S++AEK HLNW+R IYDYMTPFVSKSPRAAY NYRDLDIGTNNK G+ SY RA+VWGLK
Sbjct: 446 QSIDAEKRHLNWVRVIYDYMTPFVSKSPRAAYNNYRDLDIGTNNKCGKTSYNRARVWGLK 505

Query: 513 YFGNNFDRLVYVKTKVDPSDFFRHEQSIPNLSGI 521
           YFG NFDRLV+VKTK+DP++FFR+EQSIP L+ I
Sbjct: 506 YFGKNFDRLVHVKTKIDPTNFFRNEQSIPTLTDI 538

BLAST of Chy12G210340 vs. NCBI nr
Match: XP_011648520.1 (cannabidiolic acid synthase [Cucumis sativus] >KAE8653301.1 hypothetical protein Csa_023300 [Cucumis sativus])

HSP 1 Score: 711 bits (1834), Expect = 3.12e-252
Identity = 340/514 (66.15%), Postives = 409/514 (79.57%), Query Frame = 0

Query: 33  STQESDSFVQCLFDYGSPSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDFPKPLAII 92
           ST+  ++F+QCL +    +   I+NLIYTP NSS+ S+LNFSIQNLRF  K  PKPLAII
Sbjct: 26  STKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQNLRFSRKQTPKPLAII 85

Query: 93  TPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVVVDLINLRSISIDVENQ 152
           TP HVSHIQA ++CSKSH  QIR RSGGHDFEGLSY+SD+PF++VDLINLRSI+IDVEN+
Sbjct: 86  TPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIVDLINLRSITIDVENE 145

Query: 153 NAWVQSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVI 212
           NAWVQSGATLGE YYRI EKSQTLAFPAGSCPTVG+GGHLSGGG+GWL+RKYGLAAD+VI
Sbjct: 146 NAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGFGWLMRKYGLAADNVI 205

Query: 213 DAYLVDANGKVHNKESMGQDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLE 272
           DA  VDANGKV+++ESMG DLFWAIRGGGGGSFGI+VAWKVKLV VPA VTIC + R+LE
Sbjct: 206 DASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVRVPATVTICGSQRSLE 265

Query: 273 EDRILKLIHEWQYVAPKLDANLLLGISLT-------------------------EEVLTI 332
           E+  +KLIH+WQY+  KLD NLLLGISLT                          E++ I
Sbjct: 266 EEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKINPTALFSSFFLGKVNELMPI 325

Query: 333 LKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKGDPLEVLLNRSRLYSEISKIKSDYVKEH 392
           L   FP+L L+KE+C EMSWI++VL M  F   +P EVLLNR+  +   +KIKSDY+K+ 
Sbjct: 326 LNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPFEVLLNRTPPFGLSTKIKSDYIKKP 385

Query: 393 IPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFPHRSDYSYLVGYIAKWKN 452
           +   A K M +RLK+QD+E++QIMF+PYGG+MSE+ +SE PFPHR+   Y +GY  KWK+
Sbjct: 386 MSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIPFPHRAGNIYKLGYYVKWKD 445

Query: 453 ESVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRASYKRAKVWGLK 512
           +S++AEK HLNWIR+IYDYMTPFVSKSPRA Y NYRDLDIG NNKYG+A+Y  A+VWG K
Sbjct: 446 QSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNYRDLDIGMNNKYGKATYSHARVWGFK 505

Query: 513 YFGNNFDRLVYVKTKVDPSDFFRHEQSIPNLSGI 521
           YFG NFDRLV++KTK+DP+DFFR+EQSIP L  I
Sbjct: 506 YFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNI 539

BLAST of Chy12G210340 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 505.4 bits (1300), Expect = 5.7e-143
Identity = 250/524 (47.71%), Postives = 351/524 (66.98%), Query Frame = 0

Query: 13  LIAFIIIVVVVLSTATTSFCSTQESDSFVQCLFDYGSPSTNPIANLIYTPTNSSYSSILN 72
           ++ F     ++LS       +     SF+QCL       +N ++ +I+TP ++S+SS+L 
Sbjct: 10  VLIFFTTSTLLLSIPHPVSANRSNQSSFLQCL-SLQLNDSNIVSKVIHTPNDTSFSSVLA 69

Query: 73  FSIQNLRFLNKDFPKPLAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDI 132
            SIQN RF   D PKP+ I+TP+  S +Q+AV C++  G  IRTRSGGHD+EGLSY++  
Sbjct: 70  SSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHK 129

Query: 133 PFVVVDLINLRSISIDVENQNAWVQSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHL 192
           PFV++DL NLRSI++DV+N++ WVQ+GAT+GELYY I +K++TLAFPAG CPTVGVGGH 
Sbjct: 130 PFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHF 189

Query: 193 SGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGQDLFWAIRGGGGGSFGIVVAWK 252
           SGGGYG L+RK+GLAADHVIDA +VDA G++  +  MG+D FWAIRGGGG SF +V++WK
Sbjct: 190 SGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWK 249

Query: 253 VKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL------------ 312
           + L+ VP+ VT+    +   E   LK+IH WQ+VA K+  +L + + L            
Sbjct: 250 IGLINVPSTVTVFNVTK-FSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYKNMVRASFP 309

Query: 313 ------TEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQK--GDPLEVLLNRSRL 372
                  + +L ++   FP+LGL ++DC EMSWI+SV+   WF +   +P+ VL  R+R 
Sbjct: 310 GLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVI---WFAELGEEPINVLTKRTRA 369

Query: 373 YSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFPHR 432
            S   K KSD+V+E +P  A+  +W RL+  + E +Q++F P+GGKMSE+ D ETPFPHR
Sbjct: 370 -SLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHR 429

Query: 433 SDYSYLVGYIAKWKNESVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNK 492
               Y + Y+  W+    + ++ ++ W+  +YD M+ FV+KSPR AY+N RDLD+G    
Sbjct: 430 KGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVG 489

Query: 493 YGRASYKRAKVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIP 517
             R+ Y+  K WG+KYF NNF+RLV VKT VDPSDFF  EQSIP
Sbjct: 490 VKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Chy12G210340 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 494.2 bits (1271), Expect = 1.3e-139
Identity = 258/539 (47.87%), Postives = 357/539 (66.23%), Query Frame = 0

Query: 10  PFGLIAFIIIVVVVLS----TATTSFCSTQESDSFVQCLFDYGSPSTNPIANLIYTPTN- 69
           P   I+ II+ V+  S    T T+S  S Q  D F+ C+    +  + P+   ++TP   
Sbjct: 6   PLPTISCIIVSVLYFSFYCITPTSSSASIQ--DQFINCV-KRNTHVSFPLEKTLFTPAKN 65

Query: 70  -SSYSSILNFSIQNLRFLNKDFPKPLAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDF 129
            S ++ +L  + QNL+FL K  PKP  I  P+H S +QA++ICSK  G   R RSGGHDF
Sbjct: 66  VSLFNQVLESTAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDF 125

Query: 130 EGLSYISDI--PFVVVDLINLRSISIDVENQNAWVQSGATLGELYYRISEKSQTLAFPAG 189
           E LSY+S I  PF+++DL  L+ I++D+E+ +AWVQ GATLGELYYRI+EKS+   FPAG
Sbjct: 126 EALSYVSRIEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAG 185

Query: 190 SCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGQDLFWAIRGGG 249
            C +VG+GG+++GGGYG L+RKYGLA D+V+D  +VDANGK+ ++ +MG+DLFWAIRGGG
Sbjct: 186 LCTSVGIGGYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGG 245

Query: 250 GGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKL----------- 309
           G SFGIV+AWK+KLV VP  VT+    +TLE+D  LK I +WQ ++ K+           
Sbjct: 246 GASFGIVLAWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLR 305

Query: 310 ----DAN-----LLLGISLTEE--VLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQK 369
               D N       LG  L E+  +L +++  FP+LGLT++DC EMSWI++ L  G F  
Sbjct: 306 AAGNDGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPT 365

Query: 370 GDPLEVLLN-RSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGK 429
           G P+E+LL  +S L  +  K  SD+VKE IP++ +KG+++RL   +     + + PYGG 
Sbjct: 366 GSPIEILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTF--LNWTPYGGM 425

Query: 430 MSEVDDSETPFPHRSDYSYLVGYIAKW-KNESVEAEKSHLNWIREIYDYMTPFVSKSPRA 489
           MS++ +S  PFPHR+   + + Y A W +N+   + K  +NWI+EIY+YM P+VS +PR 
Sbjct: 426 MSKIPESAIPFPHRNGTLFKILYYANWLENDKTSSRK--INWIKEIYNYMAPYVSSNPRQ 485

Query: 490 AYVNYRDLDIGTNNKYGRASYKRAKVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIP 517
           AYVNYRDLD G N    + ++  AK+WG KYF  NFDRLV +KTKVDP +FFRHEQSIP
Sbjct: 486 AYVNYRDLDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIP 537

BLAST of Chy12G210340 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 492.3 bits (1266), Expect = 5.0e-139
Identity = 250/537 (46.55%), Postives = 362/537 (67.41%), Query Frame = 0

Query: 11  FGLIAFIIIVVVVLSTATTSFCSTQES--DSFVQCLFDYGSPSTNPIANLIYTPTNSSYS 70
           F L  FI  +++ LS +     S Q +  ++F++CL           + +I+T  + SY 
Sbjct: 6   FLLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCL---SHRINEDDSRIIHTSKDPSYF 65

Query: 71  SILNFSIQNLRFLNKDFPKPLAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSY 130
           SILN SIQN RF   + PKP++IITP+  + +Q+ + C++ HG  IRTRSGGHD+EGLSY
Sbjct: 66  SILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSY 125

Query: 131 IS-DIPFVVVDLINLRSISIDVENQNAWVQSGATLGELYYRISEKSQTLAFPAGSCPTVG 190
           ++   PFVV+DL NLRSI++DV+N+  WVQSGAT+GELYY I + S++LAFPAG  PTVG
Sbjct: 126 MAKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVG 185

Query: 191 VGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGQDLFWAIRGGGGGSFGI 250
           +GG   GGGYG L+RKYGL+AD+VIDA++VDANG   +++ MG+D FWAIRGGGG SF +
Sbjct: 186 IGGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSV 245

Query: 251 VVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTEE---- 310
           V++WK++L++VP+ VT+ +  +T E++ +  +I++WQY+A K+  +L +   L +E    
Sbjct: 246 VLSWKIRLLDVPSVVTVFKVVKTSEKEAV-SIINKWQYIADKVPNDLFIRAMLQKETEVY 305

Query: 311 -------------VLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKGDPLEVLLNRS 370
                        +L ++K  FP+LGL   +C EMSWI+SVL   WF KG+ +E+L  R 
Sbjct: 306 ASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL---WFIKGESMEILAKRK 365

Query: 371 RLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFP 430
           R  S   K K D+++E IP  A++ +W R ++ +  L++I+  P+GGKMSE+ D+E PFP
Sbjct: 366 RT-SRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFP 425

Query: 431 HRSDYSYLVGYIAKWKNESVEAE---KSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDI 490
           HR    Y + Y+A W  E  + +   + +L W+  +Y++MTP+VSKSPR AYVN+RD+D+
Sbjct: 426 HREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDL 485

Query: 491 GTNNKYG-RASYKRAKVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPNLSGISE 524
           G       +  Y+ AKVWG+KYF NNFDRLV VKT VDP DFF  EQSIP +  +++
Sbjct: 486 GMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIMKYVND 534

BLAST of Chy12G210340 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 489.2 bits (1258), Expect = 4.2e-138
Identity = 242/509 (47.54%), Postives = 348/509 (68.37%), Query Frame = 0

Query: 38  DSFVQCLFDYGSPSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDFPKPLAIITPLHV 97
           + F++CL  Y          +I+T  +SS+ SIL+ SIQN RF   + PKP++IITP+  
Sbjct: 29  EDFLKCL-SYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETPKPVSIITPVKA 88

Query: 98  SHIQAAVICSKSHGFQIRTRSGGHDFEGLSYIS-DIPFVVVDLINLRSISIDVENQNAWV 157
           S +Q  + C++ HG  +RTRS GH +EGLSYI+ + PF V+DL NLRSIS+DV+N+  WV
Sbjct: 89  SDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSISLDVDNRTGWV 148

Query: 158 QSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYL 217
           Q+GAT GELYY I + +++LAFPAG  PTVGVGG  SGGGYG L+RKYGLAAD++IDA +
Sbjct: 149 QTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGLAADNIIDALV 208

Query: 218 VDANGKVHNKESMGQDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRI 277
           VDA+G++ ++++MG+D FWAIRGGGG SFG++++WKVKLV+VP+ +T+ +  +T +++ +
Sbjct: 209 VDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFKVQKTSKKEAV 268

Query: 278 LKLIHEWQYVAPKLDANLLLGISL-------------------TEEVLTILKPTFPQLGL 337
            ++I +WQY A K+  +L +  +L                      +L +++  FP+LGL
Sbjct: 269 -RIIKKWQYAADKVPDDLFIRTTLERSNKNAVHALFTGLYIGPVNNLLALMEEKFPELGL 328

Query: 338 TKEDCLEMSWIQSVLLMGWFQKGDPLEVLLNRSRLYSEISKIKSDYVKEHIPMVAVKGMW 397
            KE C EMSWI+SVL    F KG+ L VL NR R  S   K K D+V+E IP  A++ +W
Sbjct: 329 EKEGCEEMSWIESVLWFADFPKGESLGVLTNRERT-SLSFKGKDDFVQEPIPEAAIQEIW 388

Query: 398 ERLKSQDVELSQIMFVPYGGKMSEVDDSETPFPHRSDYSYLVGYIAKWKNE----SVEAE 457
            RL++ +  L +I+  P+GGKMSE+ + ETPFPHR    Y + Y+A W+ E      E +
Sbjct: 389 RRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWREEEDKNKTETD 448

Query: 458 KSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRASYKRAKVWGLKYFGNNF 517
           K +L W+  +Y++MTP+VSKSPR AYVN++D+D+G      +  Y+  K WG+KYF NNF
Sbjct: 449 K-YLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNF 508

Query: 518 DRLVYVKTKVDPSDFFRHEQSIPNLSGIS 523
           +RLV VKT+VDP+DFF  EQSIP ++ ++
Sbjct: 509 ERLVRVKTRVDPTDFFCDEQSIPLVNKVT 533

BLAST of Chy12G210340 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 485.3 bits (1248), Expect = 6.1e-137
Identity = 241/535 (45.05%), Postives = 353/535 (65.98%), Query Frame = 0

Query: 10  PFGLIAFIIIVVVVLS-TATTSFCSTQESDSFVQCLFDYGSPSTNPIANLIYTPTN--SS 69
           P  L + +++   + + T T+S  S Q  D F+ C+    +    P+    + PT   S 
Sbjct: 6   PLPLFSILVLYFSLYTITPTSSLASLQ--DQFINCV-QRNTHVYFPLEKTFFAPTKNVSM 65

Query: 70  YSSILNFSIQNLRFLNKDFPKPLAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGL 129
           +S +L  + QNLRFL K  PKP  I +P+H SH+QA++ICSK     +R RSGGHD+EGL
Sbjct: 66  FSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGL 125

Query: 130 SYISDI--PFVVVDLINLRSISIDVENQNAWVQSGATLGELYYRISEKSQTLAFPAGSCP 189
           SY+S I  PF+++DL  +R ++I++++ +AWVQSGAT+GELYYRI+EKS+   FPAG C 
Sbjct: 126 SYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCS 185

Query: 190 TVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGQDLFWAIRGGGGGS 249
           ++G+GGH++GG YG ++RKYGL AD+V+DA +VDANGK+ ++ +MG+D FWAIRGG GGS
Sbjct: 186 SLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGS 245

Query: 250 FGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLT--- 309
           FGI++AWK+KLV VP  VT+    +TL++D   K+I +WQ VA KL   L + +      
Sbjct: 246 FGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAG 305

Query: 310 -------------------EEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKGDP 369
                                ++ ++K +FP+LGLT +DC+EMSW++S+  +  F    P
Sbjct: 306 TGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTP 365

Query: 370 LEVLLNRSRLYSEIS-KIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSE 429
             VLL     + ++S K KSD+VK  IP   ++G++++L  +D+ L  +++ PYGG M++
Sbjct: 366 TNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGGMMAK 425

Query: 430 VDDSETPFPHRSDYSYLVGYIAKWKNESVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVN 489
           + +S+ PFPHR    + V Y+  W  +S +    H+NWIR++Y YMTP+VS +PR AYVN
Sbjct: 426 IPESQIPFPHRKGVLFKVQYVTSWL-DSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVN 485

Query: 490 YRDLDIGTNNKYGRASYKRAKVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIP 517
           YRDLD+G N K  +   K+A+VWG  YF NNF+RL+ +K KVDP +FFRHEQSIP
Sbjct: 486 YRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 534