Chy12G208670 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G208670
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Ervatamin-B-like
Location	chrH12: 2975660 .. 2977084 (+)
RNA-Seq Expression	Chy12G208670
Synteny	Chy12G208670

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACCATGATGAAATTTCTTATTGTTTCCCTTGTTTTGATTGCTTTCACCTCTCACCTATGTAAGAGCTTTGAGCTAGAAAGAAAGGATTTTGAATCTGAAAAAAGTTTGATGCAACTCTACAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGCACATGAGACGGACAAACGTTTCAAGGTTTTCAAAGATAATGCAAAACATGTGTTCAAAGTGAACCACATGGGAAAATCATTAAAATTGAAGCTTAACCAATTTGCTGATATGTCTGATGATGAGTTTAGTAAGACATATGGTTCCAACATTACTTACTACAAAAACTTACATGCCAAGGCTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAACAAATATTCCATCTTCAATCGACTGGAGAAAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCAGATGTGGTAATTAATTTAATTTAATTTATAATTCTTTCTAAACTCTAATGCCTTCTAACTAATAATGGTAATTAATTTAATTTAATTTATAATTCTTTCTAAACTCTAATGCCTTCTAACTAATAATTATGTGTTATGTGTTCCGCAGGAAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAAAACAAATGAGTTAATATCTCTATCAGAGCAAGAAGTGGTGGATTGTGATTATAAAGTCGGTGGTTGTCGTGGAGGAGATTATAACTCTGCATTTGAGTTCATAATGGAAAATGGTGGAGTCACAATTGAGGATAACTATCCGTATTATGCCGGAGATGGATATTGTCGTAGACCAGGAGTGAGTTTAAATCAATATAATTCTAATTAAACTATCTAAATTTATTATATAAGTGAGTTATTGATCATAATATAATTATGTGTATTGTGTTTGAATTATACTTCACTAATAGCCTAATAATGAGAGAGTGACAATTGATGGATACGAGAACGTACCTCGAAACAATGAGTATGCTTTGATGAAAGCAGTTGCACACCAACCGGTAGCAGTGTCTATAGCGTCAAGTGGAAGTGACTTTAGATTTTATGGAGAGGCAAGTGTAGTTAATTTTGTTGTTATAATTTAATTATAATTTAATTATAATTTGGTGCAACATAATGAAAAGAAAAAATTGATAATTATTTGCAGGGAATGTTTACGGAAGAGAACTTTTGCGGAATTAGAATTGACCACACGGTAGTGGTAGTTGGGTACGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGGAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAGCACAAAGCCCACAAGGTGTATGTGGAATGGCAATGCAACCTGCCTTTCCCGTCAAGTATTGATTTCCTATCTCTTTTTTATTAGAACTGGGTAG

mRNA sequence

ATGACCATGATGAAATTTCTTATTGTTTCCCTTGTTTTGATTGCTTTCACCTCTCACCTATGTAAGAGCTTTGAGCTAGAAAGAAAGGATTTTGAATCTGAAAAAAGTTTGATGCAACTCTACAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGCACATGAGACGGACAAACGTTTCAAGGTTTTCAAAGATAATGCAAAACATGTGTTCAAAGTGAACCACATGGGAAAATCATTAAAATTGAAGCTTAACCAATTTGCTGATATGTCTGATGATGAGTTTAGTAAGACATATGGTTCCAACATTACTTACTACAAAAACTTACATGCCAAGGCTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAACAAATATTCCATCTTCAATCGACTGGAGAAAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCAGATGTGGAAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAAAACAAATGAGTTAATATCTCTATCAGAGCAAGAAGTGGTGGATTGTGATTATAAAGTCGGTGGTTGTCGTGGAGGAGATTATAACTCTGCATTTGAGTTCATAATGGAAAATGGTGGAGTCACAATTGAGGATAACTATCCGTATTATGCCGGAGATGGATATTGTCGTAGACCAGGACCTAATAATGAGAGAGTGACAATTGATGGATACGAGAACGTACCTCGAAACAATGAGTATGCTTTGATGAAAGCAGTTGCACACCAACCGGTAGCAGTGTCTATAGCGTCAAAAAAAATTGATAATTATTTGCAGGGAATGTTTACGGAAGAGAACTTTTGCGGAATTAGAATTGACCACACGGTAGTGGTAGTTGGGTACGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGGAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAGCACAAAGCCCACAAGGTGTATGTGGAATGGCAATGCAACCTGCCTTTCCCGTCAAGTATTGATTTCCTATCTCTTTTTTATTAGAACTGGGTAG

Coding sequence (CDS)

ATGACCATGATGAAATTTCTTATTGTTTCCCTTGTTTTGATTGCTTTCACCTCTCACCTATGTAAGAGCTTTGAGCTAGAAAGAAAGGATTTTGAATCTGAAAAAAGTTTGATGCAACTCTACAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGCACATGAGACGGACAAACGTTTCAAGGTTTTCAAAGATAATGCAAAACATGTGTTCAAAGTGAACCACATGGGAAAATCATTAAAATTGAAGCTTAACCAATTTGCTGATATGTCTGATGATGAGTTTAGTAAGACATATGGTTCCAACATTACTTACTACAAAAACTTACATGCCAAGGCTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAACAAATATTCCATCTTCAATCGACTGGAGAAAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCAGATGTGGAAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAAAACAAATGAGTTAATATCTCTATCAGAGCAAGAAGTGGTGGATTGTGATTATAAAGTCGGTGGTTGTCGTGGAGGAGATTATAACTCTGCATTTGAGTTCATAATGGAAAATGGTGGAGTCACAATTGAGGATAACTATCCGTATTATGCCGGAGATGGATATTGTCGTAGACCAGGACCTAATAATGAGAGAGTGACAATTGATGGATACGAGAACGTACCTCGAAACAATGAGTATGCTTTGATGAAAGCAGTTGCACACCAACCGGTAGCAGTGTCTATAGCGTCAAAAAAAATTGATAATTATTTGCAGGGAATGTTTACGGAAGAGAACTTTTGCGGAATTAGAATTGACCACACGGTAGTGGTAGTTGGGTACGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGGAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAGCACAAAGCCCACAAGGTGTATGTGGAATGGCAATGCAACCTGCCTTTCCCGTCAAGTATTGATTTCCTATCTCTTTTTTATTAGAACTGGGTAG

Protein sequence

MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGFMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYENVPRNNEYALMKAVAHQPVAVSIASKKIDNYLQGMFTEENFCGIRIDHTVVVVGYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVKY*FPISFLLELGX

Homology

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BLAST of Chy12G208670 vs. ExPASy Swiss-Prot
Match: O65039 (Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 4.2e-94
Identity = 174/347 (50.14%), Postives = 237/347 (68.30%), Query Frame = 0


Query: 3   MMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFK 62
           M KF++++L L A    + +SF+   K+ ESE+SL  LY+RW SHH +SR+ HE  KRF 
Sbjct: 1   MQKFILLALSL-ALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFN 60

Query: 63  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVGGF 122
           VFK NA HV   N M K  KLKLN+FADM++ EF  TY GS + +++       G  G F
Sbjct: 61  VFKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMFRGGPRGN-GTF 120

Query: 123 MYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEV 182
           MYE+   +P+S+DWRKKGAV ++K+QG+CGSCWAF+ + AVE I+QIKTN+L+SLSEQE+
Sbjct: 121 MYEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQEL 180

Query: 183 VDCDYKVG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 242
           VDCD     GC GG  + AFEFI + GG+T E NYPY A DG C     N   V+IDG+E
Sbjct: 181 VDCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAVSIDGHE 240

Query: 243 NVPRNNEYALMKAVAHQPVAVSI--ASKKIDNYLQGMFTEENFCGIRIDHTVVVVGYGSD 302
           NVP N+E AL+KAVA+QPV+V+I         Y +G+FT    CG  +DH V +VGYG+ 
Sbjct: 241 NVPENDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFT--GSCGTELDHGVAIVGYGTT 300

Query: 303 EEG-DYWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVK 345
            +G  YW ++N +G +WG  GY++M+RG    +G+CG+AM+ ++P+K
Sbjct: 301 IDGTKYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIAMEASYPIK 343

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BLAST of Chy12G208670 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 3.9e-92
Identity = 175/349 (50.14%), Postives = 234/349 (67.05%), Query Frame = 0


Query: 1   MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 60
           M M K L V L L +    +  SF+   KD ESE+SL  LY+RW SHH +SR+  E  KR
Sbjct: 1   MAMKKLLWVVLSL-SLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKR 60

Query: 61  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVG 120
           F VFK N  HV   N M K  KLKLN+FADM++ EF  TY GS + ++K       G  G
Sbjct: 61  FNVFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHGS-G 120

Query: 121 GFMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQ 180
            FMYE+  ++P+S+DWRKKGAV  +K+QG+CGSCWAF+ + AVE I+QIKTN+L+SLSEQ
Sbjct: 121 TFMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQ 180

Query: 181 EVVDCDYKVG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDG 240
           E+VDCD +   GC GG   SAFEFI + GG+T E NYPY A +G C     N+  V+IDG
Sbjct: 181 ELVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDG 240

Query: 241 YENVPRNNEYALMKAVAHQPVAVSI--ASKKIDNYLQGMFTEENFCGIRIDHTVVVVGYG 300
           +ENVP N+E AL+KAVA+QPV+V+I         Y +G+FT +  C   ++H V +VGYG
Sbjct: 241 HENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGD--CNTDLNHGVAIVGYG 300

Query: 301 SDEEG-DYWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVK 345
           +  +G +YWI+RN +G +WG  GY++MQR     +G+CG+AM  ++P+K
Sbjct: 301 TTVDGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIK 345

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BLAST of Chy12G208670 vs. ExPASy Swiss-Prot
Match: Q9STL4 (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 PE=1 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 4.3e-91
Identity = 171/347 (49.28%), Postives = 231/347 (66.57%), Query Frame = 0


Query: 3   MMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFK 62
           M K L++ L  +      C  F+ + K+ ESE+ L  LY RW SHH + R+ +E +KRF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 63  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHA-KAGGRVGG 122
           VF+ N  HV   N   +S KLKLN+FAD++ +EF   Y GSNI +++ L   K G +   
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFM 120

Query: 123 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 182
           + +E  + +PSS+DWRKKGAV  IKNQG+CGSCWAF+ VAAVE I++IKTN+L+SLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 183 VVDCDYKVG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGY 242
           +VDCD K   GC GG    AFEFI +NGG+T ED+YPY   DG C     N   VTIDG+
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 243 ENVPRNNEYALMKAVAHQPVAVSI--ASKKIDNYLQGMFTEENFCGIRIDHTVVVVGYGS 302
           E+VP N+E AL+KAVA+QPV+V+I   S     Y +G+FT    CG  ++H V  VGYGS
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFT--GSCGTELNHGVAAVGYGS 300

Query: 303 DEEGDYWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVK 345
           +    YWI+RN +G +WG  GY+K++R    P+G CG+AM+ ++P+K
Sbjct: 301 ERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

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BLAST of Chy12G208670 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 335.5 bits (859), Expect = 7.4e-91
Identity = 164/329 (49.85%), Postives = 222/329 (67.48%), Query Frame = 0


Query: 20  LCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFKVFKDNAKHVFKVNHMGK 79
           +  SF+   KD  SE+SL  LY+RW SHH +SR+  E  KRF VFK N  HV   N M K
Sbjct: 19  VANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKRFNVFKANLMHVHNTNKMDK 78

Query: 80  SLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGFMYERATNIPSSIDWRKKG 139
             KLKLN+FADM++ EF  TY  +   +  +        G FMYE+  ++P S+DWRKKG
Sbjct: 79  PYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTPHENGAFMYEKVVSVPPSVDWRKKG 138

Query: 140 AVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDYKVG-GCRGGDYNS 199
           AV  +K+QG+CGSCWAF+ V AVE I+QIKTN+L++LSEQE+VDCD +   GC GG   S
Sbjct: 139 AVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQELVDCDKEENQGCNGGLMES 198

Query: 200 AFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYENVPRNNEYALMKAVAHQP 259
           AFEFI + GG+T E NYPY A +G C     N+  V+IDG+ENVP N+E AL+KAVA+QP
Sbjct: 199 AFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGHENVPANDEDALLKAVANQP 258

Query: 260 VAVSI--ASKKIDNYLQGMFTEENFCGIRIDHTVVVVGYGSDEEG-DYWIIRNQYGTQWG 319
           V+V+I         Y +G+FT +  C   ++H V +VGYG+  +G +YWI+RN +G +WG
Sbjct: 259 VSVAIDAGGSDFQFYSEGVFTGD--CSTDLNHGVAIVGYGTTVDGTNYWIVRNSWGPEWG 318

Query: 320 MNGYMKMQRGAQSPQGVCGMAMQPAFPVK 345
            +GY++MQR     +G+CG+AM P++P+K
Sbjct: 319 EHGYIRMQRNISKKEGLCGIAMLPSYPIK 345

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BLAST of Chy12G208670 vs. ExPASy Swiss-Prot
Match: Q9STL5 (KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 PE=2 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.1e-90
Identity = 168/342 (49.12%), Postives = 233/342 (68.13%), Query Frame = 0


Query: 11  LVLIAFTSHL--CKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFKVFKDNA 70
           +VLI+F S L   K F+ + K+ E+E+++ +LY+RW  HH +SR +HE  KRF VF+ N 
Sbjct: 6   IVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFRHNV 65

Query: 71  KHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVGGFMYERAT 130
            HV + N   K  KLK+N+FAD++  EF  +Y GSN+ +++ L     G  GGFMYE  T
Sbjct: 66  LHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGS-GGFMYENVT 125

Query: 131 NIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDYK 190
            +PSS+DWR+KGAV  +KNQ  CGSCWAF+ VAAVE I++I+TN+L+SLSEQE+VDCD +
Sbjct: 126 RVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDCDTE 185

Query: 191 VG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGD-GYCRRPGPNNERVTIDGYENVPRN 250
              GC GG    AFEFI  NGG+  E+ YPY + D  +CR      E VTIDG+E+VP N
Sbjct: 186 ENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHVPEN 245

Query: 251 NEYALMKAVAHQPVAVSI--ASKKIDNYLQGMFTEENFCGIRIDHTVVVVGYGSDEEG-D 310
           +E  L+KAVAHQPV+V+I   S     Y +G+F  E  CG +++H VV+VGYG  + G  
Sbjct: 246 DEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGE--CGTQLNHGVVIVGYGETKNGTK 305

Query: 311 YWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVK 345
           YWI+RN +G +WG  GY++++RG    +G CG+AM+ ++P K
Sbjct: 306 YWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

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BLAST of Chy12G208670 vs. ExPASy TrEMBL
Match: A0A5D3D043 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001320 PE=3 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 6.3e-170
Identity = 297/350 (84.86%), Postives = 320/350 (91.43%), Query Frame = 0


Query: 1   MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 60
           M +MKFLIV LVLIAFT HLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E  KR
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGG 120
           FKVFKDNAK+VFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK  GRVGG
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAK-NGRVGG 120

Query: 121 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 180
           FMYE A +IPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNEL+SLSEQE
Sbjct: 121 FMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180

Query: 181 VVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 240
           VVDCDY+  GC GG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERVTIDGYE
Sbjct: 181 VVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDGYE 240

Query: 241 NVPRNNEYALMKAVAHQPVAVSIASK----KIDNYLQGMFTEENFCGIRIDHTVVVVGYG 300
           NVPRNNE+ALMKAVAHQPVAV+IAS       DNYLQGMFTE++FCG  IDHTVVVVGYG
Sbjct: 241 NVPRNNEHALMKAVAHQPVAVAIASSGRILNFDNYLQGMFTEQDFCGYNIDHTVVVVGYG 300

Query: 301 SDEE-GDYWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVKY 346
           +DEE GDYWIIRNQYGTQWGMNGYMKMQRGA++PQGVCGMA+QPA+PVK+
Sbjct: 301 TDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 349

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BLAST of Chy12G208670 vs. ExPASy TrEMBL
Match: A0A0A0LY73 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G528520 PE=3 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 6.3e-170
Identity = 298/343 (86.88%), Postives = 308/343 (89.80%), Query Frame = 0


Query: 3   MMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFK 62
           MMKFLIV LVLIAF SH+C+SFELERKDFESEKSLMQLYKRWSSHHRISRN HE D+RFK
Sbjct: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60

Query: 63  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGFM 122
           VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAK GGRVGGFM
Sbjct: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFM 120

Query: 123 YERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVV 182
           YERATNIPSSIDWRKKGA        R   CWAFAAVAAVESIHQI+TNEL+SLSEQEVV
Sbjct: 121 YERATNIPSSIDWRKKGA--------RRMCCWAFAAVAAVESIHQIRTNELVSLSEQEVV 180

Query: 183 DCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYENV 242
           DCDYKVGGCRGGDY SAFEFIMENGG+T+E+NYPYYAGDGYCRR GPNNERVTIDGYENV
Sbjct: 181 DCDYKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENV 240

Query: 243 PRNNEYALMKAVAHQPVAVSIASKKIDNYLQGMFTEENFCGIRIDHTVVVVGYGSDEEGD 302
           PRNNEYALMKAVAHQP               GMFTEENFCGIRIDHTVVVVGYGSDEEGD
Sbjct: 241 PRNNEYALMKAVAHQP---------------GMFTEENFCGIRIDHTVVVVGYGSDEEGD 300

Query: 303 YWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVKY 346
           YWIIRNQYGTQWGMNGYMKMQRG ++PQGVCGMAM PAFPVKY
Sbjct: 301 YWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVKY 320

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BLAST of Chy12G208670 vs. ExPASy TrEMBL
Match: A0A1S3BYQ3 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494879 PE=3 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.8e-169
Identity = 298/347 (85.88%), Postives = 316/347 (91.07%), Query Frame = 0


Query: 2   TMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRF 61
           T+MKFLIV LVLIA TSHLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E  KRF
Sbjct: 3   TVMKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 62  KVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGF 121
           KVFKDNAKHVFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK  G VGGF
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKT-GHVGGF 122

Query: 122 MYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEV 181
           MYE A  IPSSIDWRKKGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNEL+SLSEQEV
Sbjct: 123 MYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQEV 182

Query: 182 VDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYEN 241
           VDCDY+ GGCRGG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERV IDGYEN
Sbjct: 183 VDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGYEN 242

Query: 242 VPRNNEYALMKAVAHQPVAVSIASKKID--NYLQGMFTEENFCGIRIDHTVVVVGYGSDE 301
           VPRNNE+ALMKAVAHQPVAV+IAS   D   Y QGMFTE++FCG  IDHTVVVVGYGSDE
Sbjct: 243 VPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYGSDE 302

Query: 302 E-GDYWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVKY 346
           E GDYWIIRNQYGTQWGMNGYMKMQRGA++PQGVCGMAMQPA+PVKY
Sbjct: 303 EDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 348

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BLAST of Chy12G208670 vs. ExPASy TrEMBL
Match: A0A5A7TM64 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003430 PE=3 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 5.4e-169
Identity = 297/347 (85.59%), Postives = 315/347 (90.78%), Query Frame = 0


Query: 2   TMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRF 61
           T+MKFLIV  VLIA TSHLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E  KRF
Sbjct: 3   TVMKFLIVPFVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 62  KVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGF 121
           KVFKDNAKHVFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK  G VGGF
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKT-GHVGGF 122

Query: 122 MYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEV 181
           MYE A  IPSSIDWRKKGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNEL+SLSEQEV
Sbjct: 123 MYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQEV 182

Query: 182 VDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYEN 241
           VDCDY+ GGCRGG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERV IDGYEN
Sbjct: 183 VDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGYEN 242

Query: 242 VPRNNEYALMKAVAHQPVAVSIASKKID--NYLQGMFTEENFCGIRIDHTVVVVGYGSDE 301
           VPRNNE+ALMKAVAHQPVAV+IAS   D   Y QGMFTE++FCG  IDHTVVVVGYGSDE
Sbjct: 243 VPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYGSDE 302

Query: 302 E-GDYWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVKY 346
           E GDYWIIRNQYGTQWGMNGYMKMQRGA++PQGVCGMAMQPA+PVKY
Sbjct: 303 EDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 348

[Close]

BLAST of Chy12G208670 vs. ExPASy TrEMBL
Match: A0A1S3BYU0 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 1.0e-167
Identity = 295/348 (84.77%), Postives = 318/348 (91.38%), Query Frame = 0


Query: 1   MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 60
           M +MKFLIV LVLIAFT HLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E  KR
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGG 120
           FKVFKDNAK+VFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK  GRVGG
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAK-NGRVGG 120

Query: 121 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 180
           FMYE A +IPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNEL+SLSEQE
Sbjct: 121 FMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180

Query: 181 VVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 240
           VVDCDY+  GC GG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERVTIDGYE
Sbjct: 181 VVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDGYE 240

Query: 241 NVPRNNEYALMKAVAHQPVAVSIASKKID--NYLQGMFTEENFCGIRIDHTVVVVGYGSD 300
           NVPRNNE+ALMKAVAHQPVAV+IAS   D   Y QGMFTE++FCG  IDHTVVVVGYG+D
Sbjct: 241 NVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYGTD 300

Query: 301 EE-GDYWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVKY 346
           EE GDYWIIRNQYGTQWGMNGYMKMQRGA++PQGVCGMA+QPA+PVK+
Sbjct: 301 EEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 347

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BLAST of Chy12G208670 vs. NCBI nr
Match: XP_031739597.1 (ervatamin-B-like [Cucumis sativus] >KAE8649107.1 hypothetical protein Csa_014529 [Cucumis sativus])

HSP 1 Score: 621 bits (1601), Expect = 7.81e-223
Identity = 302/349 (86.53%), Postives = 324/349 (92.84%), Query Frame = 0


Query: 1   MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 60
           MTMMKFLIV +VLIAFTSHLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNAHE  KR
Sbjct: 1   MTMMKFLIVFVVLIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGG--RV 120
           FK+F+DNAKHVF+VNHMGKSLKL+LNQFAD+SDDEFS  YGSNIT+Y NLHAKAGG  RV
Sbjct: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRV 120

Query: 121 GGFMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSE 180
           GGFMYERA  IPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNEL+SLSE
Sbjct: 121 GGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 180

Query: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDG 240
           QEVVDCDYKVGGCRGGDYNSAFEFIM+NGG+TIE+NYPY+AG+GYCRR GPNNERVTIDG
Sbjct: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDG 240

Query: 241 YENVPRNNEYALMKAVAHQPVAVSIASKKIDN--YLQGMFTEENFCGIRIDHTVVVVGYG 300
           YE VPRNNEYALMKAVAHQPVAV++AS   D   Y +GM  E +FCG RIDHTVVVVGYG
Sbjct: 241 YERVPRNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYG 300

Query: 301 SDEEGDYWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVKY 345
           SDEEGDYWIIRNQYGTQWGMNGYMKMQRG ++PQGVCGMAMQP+FPVKY
Sbjct: 301 SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 349

[Close]

BLAST of Chy12G208670 vs. NCBI nr
Match: XP_031740503.1 (ervatamin-B [Cucumis sativus])

HSP 1 Score: 618 bits (1594), Expect = 8.45e-222
Identity = 298/347 (85.88%), Postives = 321/347 (92.51%), Query Frame = 0


Query: 1   MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 60
           MTMMKFLIV +VLIAF SHLC+ F+LERKDFESEKSLMQLYKRWSSHHRISRNAHE  KR
Sbjct: 1   MTMMKFLIVFVVLIAFASHLCEGFDLERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGG 120
           FK+F+DNAK VFKVNHMGKSLKL+LNQFAD+SDDEFS  YGSNIT+Y NLHAKAGGRVGG
Sbjct: 61  FKIFQDNAKRVFKVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNNLHAKAGGRVGG 120

Query: 121 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 180
           FMYERA NIP SIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNEL+SLSEQE
Sbjct: 121 FMYERAMNIPFSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180

Query: 181 VVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 240
           VVDCDYKVGGCRGGDYNSAFEFIM+NGG+TIE+NYPY+AG+GYCRR GPN+ERVTIDGYE
Sbjct: 181 VVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYE 240

Query: 241 NVPRNNEYALMKAVAHQPVAVSIASKKIDN--YLQGMFTEENFCGIRIDHTVVVVGYGSD 300
            VP+NNEYALMKAVAHQPVAVS+AS   D   Y +GM  E +FCG RIDHTVVVVGYGSD
Sbjct: 241 RVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGSD 300

Query: 301 EEGDYWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVKY 345
           EEGDYWIIRNQYGTQWGMNGYMKMQRG ++PQGVCGMAMQP+FPVKY
Sbjct: 301 EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 347

[Close]

BLAST of Chy12G208670 vs. NCBI nr
Match: XP_031740474.1 (ervatamin-B [Cucumis sativus] >KAE8649110.1 hypothetical protein Csa_014565 [Cucumis sativus])

HSP 1 Score: 605 bits (1559), Expect = 1.62e-216
Identity = 292/347 (84.15%), Postives = 322/347 (92.80%), Query Frame = 0


Query: 1   MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 60
           MTMMKFLIV +VLIAFTSHLC+SFELE KDFESE+SLMQLYKRWSSHHRISRNAHE  KR
Sbjct: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGG 120
           FK+F+DNAKHVF+VNHMGKSLKL+LNQFAD+SDDEFS  YGSNIT+Y +LHA    RVGG
Sbjct: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN---RVGG 120

Query: 121 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 180
           FMYERA NIPSSIDWR++GAVNAIKNQGRCGSCWAFAAVAAVESIHQI+TNEL+SLSEQE
Sbjct: 121 FMYERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQE 180

Query: 181 VVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 240
           VVDCDYKVGGCRGG+Y+SAFEFIM+NGG+TIE+NYPY+AG+GYCRR GPN+ERVTIDGYE
Sbjct: 181 VVDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYE 240

Query: 241 NVPRNNEYALMKAVAHQPVAVSIASKKIDN--YLQGMFTEENFCGIRIDHTVVVVGYGSD 300
            VP+NNEYALMKAVAHQPVAVS+AS   D   Y +GM  E++FCG RIDHTVVVVGYGSD
Sbjct: 241 CVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGSD 300

Query: 301 EEGDYWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVKY 345
           EEGDYWIIRNQYGTQWGMNGYMKMQRG ++PQGVCGMAMQP+FPVKY
Sbjct: 301 EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 344

[Close]

BLAST of Chy12G208670 vs. NCBI nr
Match: TYK16990.1 (ervatamin-B-like [Cucumis melo var. makuwa])

HSP 1 Score: 605 bits (1559), Expect = 1.95e-216
Identity = 297/350 (84.86%), Postives = 320/350 (91.43%), Query Frame = 0


Query: 1   MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 60
           M +MKFLIV LVLIAFT HLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E  KR
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGG 120
           FKVFKDNAK+VFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK  GRVGG
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAK-NGRVGG 120

Query: 121 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 180
           FMYE A +IPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNEL+SLSEQE
Sbjct: 121 FMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180

Query: 181 VVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 240
           VVDCDY+  GC GG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERVTIDGYE
Sbjct: 181 VVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDGYE 240

Query: 241 NVPRNNEYALMKAVAHQPVAVSIASK----KIDNYLQGMFTEENFCGIRIDHTVVVVGYG 300
           NVPRNNE+ALMKAVAHQPVAV+IAS       DNYLQGMFTE++FCG  IDHTVVVVGYG
Sbjct: 241 NVPRNNEHALMKAVAHQPVAVAIASSGRILNFDNYLQGMFTEQDFCGYNIDHTVVVVGYG 300

Query: 301 SDEE-GDYWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVKY 345
           +DEE GDYWIIRNQYGTQWGMNGYMKMQRGA++PQGVCGMA+QPA+PVK+
Sbjct: 301 TDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 349

[Close]

BLAST of Chy12G208670 vs. NCBI nr
Match: XP_008454483.1 (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 603 bits (1554), Expect = 1.09e-215
Identity = 298/347 (85.88%), Postives = 316/347 (91.07%), Query Frame = 0


Query: 2   TMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRF 61
           T+MKFLIV LVLIA TSHLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E  KRF
Sbjct: 3   TVMKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 62  KVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGF 121
           KVFKDNAKHVFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK G  VGGF
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKTG-HVGGF 122

Query: 122 MYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEV 181
           MYE A  IPSSIDWRKKGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNEL+SLSEQEV
Sbjct: 123 MYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQEV 182

Query: 182 VDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYEN 241
           VDCDY+ GGCRGG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERV IDGYEN
Sbjct: 183 VDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGYEN 242

Query: 242 VPRNNEYALMKAVAHQPVAVSIASKKIDN--YLQGMFTEENFCGIRIDHTVVVVGYGSDE 301
           VPRNNE+ALMKAVAHQPVAV+IAS   D   Y QGMFTE++FCG  IDHTVVVVGYGSDE
Sbjct: 243 VPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYGSDE 302

Query: 302 E-GDYWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVKY 345
           E GDYWIIRNQYGTQWGMNGYMKMQRGA++PQGVCGMAMQPA+PVKY
Sbjct: 303 EDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 348

[Close]

BLAST of Chy12G208670 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 336.3 bits (861), Expect = 3.1e-92
Identity = 171/347 (49.28%), Postives = 231/347 (66.57%), Query Frame = 0


Query: 3   MMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFK 62
           M K L++ L  +      C  F+ + K+ ESE+ L  LY RW SHH + R+ +E +KRF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 63  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHA-KAGGRVGG 122
           VF+ N  HV   N   +S KLKLN+FAD++ +EF   Y GSNI +++ L   K G +   
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFM 120

Query: 123 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 182
           + +E  + +PSS+DWRKKGAV  IKNQG+CGSCWAF+ VAAVE I++IKTN+L+SLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 183 VVDCDYKVG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGY 242
           +VDCD K   GC GG    AFEFI +NGG+T ED+YPY   DG C     N   VTIDG+
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 243 ENVPRNNEYALMKAVAHQPVAVSI--ASKKIDNYLQGMFTEENFCGIRIDHTVVVVGYGS 302
           E+VP N+E AL+KAVA+QPV+V+I   S     Y +G+FT    CG  ++H V  VGYGS
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFT--GSCGTELNHGVAAVGYGS 300

Query: 303 DEEGDYWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVK 345
           +    YWI+RN +G +WG  GY+K++R    P+G CG+AM+ ++P+K
Sbjct: 301 ERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

[Close]

BLAST of Chy12G208670 vs. TAIR 10
Match: AT3G48350.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 334.0 bits (855), Expect = 1.5e-91
Identity = 168/342 (49.12%), Postives = 233/342 (68.13%), Query Frame = 0


Query: 11  LVLIAFTSHL--CKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFKVFKDNA 70
           +VLI+F S L   K F+ + K+ E+E+++ +LY+RW  HH +SR +HE  KRF VF+ N 
Sbjct: 6   IVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFRHNV 65

Query: 71  KHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVGGFMYERAT 130
            HV + N   K  KLK+N+FAD++  EF  +Y GSN+ +++ L     G  GGFMYE  T
Sbjct: 66  LHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGS-GGFMYENVT 125

Query: 131 NIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDYK 190
            +PSS+DWR+KGAV  +KNQ  CGSCWAF+ VAAVE I++I+TN+L+SLSEQE+VDCD +
Sbjct: 126 RVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDCDTE 185

Query: 191 VG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGD-GYCRRPGPNNERVTIDGYENVPRN 250
              GC GG    AFEFI  NGG+  E+ YPY + D  +CR      E VTIDG+E+VP N
Sbjct: 186 ENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHVPEN 245

Query: 251 NEYALMKAVAHQPVAVSI--ASKKIDNYLQGMFTEENFCGIRIDHTVVVVGYGSDEEG-D 310
           +E  L+KAVAHQPV+V+I   S     Y +G+F  E  CG +++H VV+VGYG  + G  
Sbjct: 246 DEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGE--CGTQLNHGVVIVGYGETKNGTK 305

Query: 311 YWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVK 345
           YWI+RN +G +WG  GY++++RG    +G CG+AM+ ++P K
Sbjct: 306 YWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

[Close]

BLAST of Chy12G208670 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 321.6 bits (823), Expect = 7.8e-88
Identity = 165/347 (47.55%), Postives = 228/347 (65.71%), Query Frame = 0


Query: 3   MMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFK 62
           M +F++++L ++       K  +   KD ESE SL +LY+RW SHH ++R+  E  KRF 
Sbjct: 1   MKRFIVLALCML-MVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFN 60

Query: 63  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVGGF 122
           VFK N KH+ + N   KS KLKLN+F DM+ +EF +TY GSNI +++    +       F
Sbjct: 61  VFKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKKA-TKSF 120

Query: 123 MYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEV 182
           MY     +P+S+DWRK GAV  +KNQG+CGSCWAF+ V AVE I+QI+T +L SLSEQE+
Sbjct: 121 MYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSEQEL 180

Query: 183 VDCDYKVG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 242
           VDCD     GC GG  + AFEFI E GG+T E  YPY A D  C     N   V+IDG+E
Sbjct: 181 VDCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGHE 240

Query: 243 NVPRNNEYALMKAVAHQPVAVSI--ASKKIDNYLQGMFTEENFCGIRIDHTVVVVGYGSD 302
           +VP+N+E  LMKAVA+QPV+V+I         Y +G+FT    CG  ++H V VVGYG+ 
Sbjct: 241 DVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFTGR--CGTELNHGVAVVGYGTT 300

Query: 303 EEG-DYWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVK 345
            +G  YWI++N +G +WG  GY++MQRG +  +G+CG+AM+ ++P+K
Sbjct: 301 IDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLK 343

[Close]

BLAST of Chy12G208670 vs. TAIR 10
Match: AT3G19400.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 290.8 bits (743), Expect = 1.5e-78
Identity = 165/353 (46.74%), Postives = 227/353 (64.31%), Query Frame = 0


Query: 7   LIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNA-HETDKRFKVFK 66
           +I+S++L++ +  +    E+ER   E+E  LM  Y++W   +R + N   E ++RFK+FK
Sbjct: 14  VILSVLLLSSSLGVATETEIERN--ETEVRLM--YEQWLVENRKNYNGLGEKERRFKIFK 73

Query: 67  DNAKHVFKVNHM-GKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGFMYE 126
           DN K V + N +  ++ ++ L +FAD++++EF   Y       K    K   +   ++Y+
Sbjct: 74  DNLKFVDEHNSVPDRTFEVGLTRFADLTNEEFRAIY----LRKKMERTKDSVKTERYLYK 133

Query: 127 RATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDC 186
               +P  +DWR  GAV ++K+QG CGSCWAF+AV AVE I+QI T ELISLSEQE+VDC
Sbjct: 134 EGDVLPDEVDWRANGAVVSVKDQGNCGSCWAFSAVGAVEGINQITTGELISLSEQELVDC 193

Query: 187 D--YKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGD-GYCRRPGPNNER-VTIDGYE 246
           D  +   GC GG  N AFEFIM+NGG+  + +YPY A D G C     NN R VTIDGYE
Sbjct: 194 DRGFVNAGCDGGIMNYAFEFIMKNGGIETDQDYPYNANDLGLCNADKNNNTRVVTIDGYE 253

Query: 247 NVPRNNEYALMKAVAHQPVAVSI--ASKKIDNYLQGMFTEENFCGIRIDHTVVVVGYGSD 306
           +VPR++E +L KAVAHQPV+V+I  +S+    Y  G+ T    CGI +DH VVVVGYGS 
Sbjct: 254 DVPRDDEKSLKKAVAHQPVSVAIEASSQAFQLYKSGVMT--GTCGISLDHGVVVVGYGST 313

Query: 307 EEGDYWIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFPVKY*FPISF 352
              DYWIIRN +G  WG +GY+K+QR    P G CG+AM P++P K  FP SF
Sbjct: 314 SGEDYWIIRNSWGLNWGDSGYVKLQRNIDDPFGKCGIAMMPSYPTKSSFPSSF 356

[Close]

BLAST of Chy12G208670 vs. TAIR 10
Match: AT5G45890.1 (senescence-associated gene 12 )

HSP 1 Score: 273.9 bits (699), Expect = 1.9e-73
Identity = 146/339 (43.07%), Postives = 209/339 (61.65%), Query Frame = 0


Query: 12  VLIAFTSHLCKSFELERKDFESEKSLMQLYKRW-SSHHRISRNAHETDKRFKVFKDNAKH 71
           + +A  S  C S  L R   ++E  + + +  W + H R+  +  E + R+ VFK+N + 
Sbjct: 10  LFVAIFSSFCFSITLSR-PLDNELIMQKRHIEWMTKHGRVYADVKEENNRYVVFKNNVER 69

Query: 72  VFKVNHM--GKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGFMYERATN 131
           +  +N +  G++ KL +NQFAD+++DEF   Y +       L +++  ++  F Y+  ++
Sbjct: 70  IEHLNSIPAGRTFKLAVNQFADLTNDEFRSMY-TGFKGVSALSSQSQTKMSPFRYQNVSS 129

Query: 132 --IPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDY 191
             +P S+DWRKKGAV  IKNQG CG CWAF+AVAA+E   QIK  +LISLSEQ++VDCD 
Sbjct: 130 GALPVSVDWRKKGAVTPIKNQGSCGCCWAFSAVAAIEGATQIKKGKLISLSEQQLVDCDT 189

Query: 192 KVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYENVPRNN 251
              GC GG  ++AFE I   GG+T E NYPY   D  C     N +  +I GYE+VP N+
Sbjct: 190 NDFGCEGGLMDTAFEHIKATGGLTTESNYPYKGEDATCNSKKTNPKATSITGYEDVPVND 249

Query: 252 EYALMKAVAHQPVAVSIASKKID--NYLQGMFTEENFCGIRIDHTVVVVGYGSDEEGD-Y 311
           E ALMKAVAHQPV+V I     D   Y  G+FT E  C   +DH V  +GYG    G  Y
Sbjct: 250 EQALMKAVAHQPVSVGIEGGGFDFQFYSSGVFTGE--CTTYLDHAVTAIGYGESTNGSKY 309

Query: 312 WIIRNQYGTQWGMNGYMKMQRGAQSPQGVCGMAMQPAFP 343
           WII+N +GT+WG +GYM++Q+  +  QG+CG+AM+ ++P
Sbjct: 310 WIIKNSWGTKWGESGYMRIQKDVKDKQGLCGLAMKASYP 344

The following BLAST results are available for this feature:

BLAST of Chy12G208670 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65039	4.2e-94	50.14	Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1	[more]
P12412	3.9e-92	50.14	Vignain OS=Vigna mungo OX=3915 PE=1 SV=1	[more]
Q9STL4	4.3e-91	49.28	KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 ...	[more]
P25803	7.4e-91	49.85	Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2	[more]
Q9STL5	2.1e-90	49.12	KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 ...	[more]

BLAST of Chy12G208670 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D043	6.3e-170	84.86	Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001...	[more]
A0A0A0LY73	6.3e-170	86.88	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G528520 PE=3 SV=1	[more]
A0A1S3BYQ3	1.8e-169	85.88	ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494879 PE=3 SV=1	[more]
A0A5A7TM64	5.4e-169	85.59	Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G0034...	[more]
A0A1S3BYU0	1.0e-167	84.77	ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1	[more]

BLAST of Chy12G208670 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739597.1	7.81e-223	86.53	ervatamin-B-like [Cucumis sativus] >KAE8649107.1 hypothetical protein Csa_014529...	[more]
XP_031740503.1	8.45e-222	85.88	ervatamin-B [Cucumis sativus]	[more]
XP_031740474.1	1.62e-216	84.15	ervatamin-B [Cucumis sativus] >KAE8649110.1 hypothetical protein Csa_014565 [Cuc...	[more]
TYK16990.1	1.95e-216	84.86	ervatamin-B-like [Cucumis melo var. makuwa]	[more]
XP_008454483.1	1.09e-215	85.88	PREDICTED: ervatamin-B-like [Cucumis melo]	[more]

BLAST of Chy12G208670 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48340.1	3.1e-92	49.28	Cysteine proteinases superfamily protein	[more]
AT3G48350.1	1.5e-91	49.12	Cysteine proteinases superfamily protein	[more]
AT5G50260.1	7.8e-88	47.55	Cysteine proteinases superfamily protein	[more]
AT3G19400.1	1.5e-78	46.74	Cysteine proteinases superfamily protein	[more]
AT5G45890.1	1.9e-73	43.07	senescence-associated gene 12	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 147..162 score: 62.45 coord: 303..309 score: 55.51 coord: 288..298 score: 52.4
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 129..343 e-value: 1.3E-91 score: 320.4
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 129..342 e-value: 5.7E-69 score: 232.5
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 41..96 e-value: 1.0E-8 score: 45.0
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 41..96 e-value: 1.3E-9 score: 38.3
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 14..345 e-value: 8.8E-102 score: 343.1
None	No IPR available	PANTHER	PTHR12411:SF706	KDEL-TAILED CYSTEINE ENDOPEPTIDASE CEP1	coord: 21..348
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 21..348
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 147..158
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 130..342 e-value: 4.04479E-94 score: 277.583
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 37..343

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G208670.1	Chy12G208670.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018108	peptidyl-tyrosine phosphorylation
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0004713	protein tyrosine kinase activity
molecular_function	GO:0008234	cysteine-type peptidase activity