CcUC06G124030 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC06G124030
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
DescriptiontRNA pseudouridine synthase
LocationCicolChr06: 26493471 .. 26498419 (-)
RNA-Seq ExpressionCcUC06G124030
SyntenyCcUC06G124030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGTCGAATTACTCAGGCCCAGATCAGCTGGGTCTATTGGGCTTTCCCAGTTGGACCAGCATGGGCTTGCCCTCTGCTTGACTGCATCTACTTGGATTTACAATCTCGTCCCTGCTGCGACGGCCGCTGCAACTGCAAGATACGGTCGTCCGTTCTCAGGTGGGGCGGTGGTTATGATACCAAATCCAAGGGCTAGACTTGTATCACCATCAGGTGCCAGTTTTGGTTTCAAGATTCTGATGGAGAATCGAGCTCAGCAAAAAGACTATCTTCACTATAACCACACTGATCGCTGCAGAAATGCTAGATGGACTGCCAGGTTTTTCTTTCGTACATCTTCATTATCTATGGCTTTATTAGATTAGAGATTTTACTCCTGGAATCAGCTTTGTGCTTGGTTGTTATGTAATCCAATCGGAATCTGAGAAGGCTGTTAATCAAGCTGTTGTTATGCATTTGGCAGGGAAAGCTACCAACTCATGTATGGAAGGCCGTGGCAAGAAGTGCTTGATTTTTACTTTCATGCTGTTAATGGAAGGTTCTCACTGACCTCGTTGTTTGGAACTGAGGCGAGTCTCCAATATCTACATTATCTTTAAACTTCGTTCCTCATTCTCTATAAATATTCCGTTTGTTAAAATGTAAGCAATTGAATCGTGAACTACGGCTCCTTTTCCCTCCCTTGCGAGCTTACGAATCCTTCTCTCTTGTAATGCTAAGAGCAGTCTTTATATATCTTAAGCCGCTTTCGTTAGACTGTTATAGTTAAGGAATCTTATTTTTTTCTGTTCAGGTTCTTTGATTCTTAAAATAATGGCTTCCATCTAAGACTGCCAATTGCTTTAACATTGTACCGTGTAGGTTCTACAATTATCTTGTACAAAACAAGGAGATATCGTTGATTTTTTTAATTTATTGTATATGATTCTTTACCCACAGACAGGTCTTGATCATGATGATGCTGAGACTCCAGCGATTTACAACACAGCAGAGTTAAACAGAGCTTCATTCAAAGATAAGGCTGGGAGGTGGGCAAGGGCAACATTTAAGATTGTTCTTTCCTACCATGGAAATTCCTTTGATGGGTGGCAAAAGCAGCCAGGCTTGAATACTGTTCAGGGGTAAGCTTATTTCATGGAAGTTACCATATAGACAAGTGATATTGTATGCAATTGGGAGTGACGTTCTTTTCACTATCAGATCTTTTAAACTGAAGAATCGTTGAGAACAAGTAATTTATAGTAAGCGTTTGAGAAGTTAGTTCTAGAGATACAAGTGAAAATCTCATGCCTTCATTTCAAATGAAACCTTTGTCTTGTGGACAATCTCCAAATTAAGTCAATCGACCTTGTTAATCTCACCTTTCTGGTACTGTATCTTCTTCTTCGAATACAATAGTTTAAAGCAATGTTTTAATATCTTTAGATGTATAGATAGTTTGATTTCAAACACTGCTGTATATTGGAGGATTATGTCTACTTGTATCCTTTGGCAGCTTGGTAGAAAAATCTCTAGGGAAGTTTGTAGATGAGAAGAAAGCTCAGATGCTGAAAGATAGATGCTTACCAATAGAAGGATGTGCTGTGGTTGCTGGGCGAACTGACAAAGGGGTGACAGCTCTGCGACAAGTTTGCTCATTCTGTATGTTGGCTTTCTAATTTCTGTTGACATGACTTTTTGATTGGATATTTAATTTCACTACTCTGCTTTCATTTTCTTAGATTCTATCTCAGCTCAAGAATGAATTCTTCTATTAGAATATAACTTTTATATTATTTCTTGACATCTTAATAGTTGCAACGAATAGCCTACCCATTAATTGCTTGTGACATCCGTTTCATGAAATCCACTTTTGTTGTGCTTCACTTAAACACTCAAATAGTCAAATAGGTGACTTTGTCCCGACCGACCTATGCTCAAAGTGTTGGAAGGGTATGCCAAAAGAGCTGTCAAAAGCAATTAGTATTGAAACAAGGGTGGTGTGAAATCTATATTTAATATTATCACAACTCTTTTATTGATTAAATTCTCAGGAACATTTTCCTTTTTAACCAAACAGTAGGAGGTACCACACTGGAACCAAACCCAGGAATAACTTCGCAATAAAAAGTAGCAAGAGTAAAAAAATTTGTACTTCACACACTGTATATATTTTTTCTAGATAAGATGCACACTGTATTTAATAAGGCTAATCTTTAGAAGCTTTGACCAAGATACCCATCACTGATTATTAATTCAAGTAGTTTGCAGCATGGAAGTTGGGAGTGCATTTCTTTTTATTACCATCTGCAGAATTCCTTATCTGATGCTACAGAAAGGAAAAGTTGGCAAAGAAGATTGAAGTTTTTATTTGAACTATTTCCAATCTGTGCCTTTATACCAATAGTAGAATTCAAGTTAGACATCCAAACTACTCGGCCATCACAGTACCAATAATAGAATTCAACCTGAACCATGCTTCCTTTGGTTTGAGTTCTTCAACATAAATATATACATGAAACTTGTTTAGAAAGAGCAATTGCTATATGTGTAGTTTTTTTAGTCTTTTCTGTGTCTTTTCATCTTTGGTTTCAAATTACCCTTGGAGCGAAACATCCTCTTTCTGACTTCACCCACTTAATTTTTCTTATTGTTTTCTATTTTAGAGAATCAATATTCGACTTCAGTTAAACTCCCTACTTGTAGATACTTGGCGCAGCGATGTTAAACCTCAAGACATTGAAGATTCTATCAATAGTGCAGCTCCTGGAAAACTCAGAACATTATCTGTTTCTCAGGTAAAATTAAGGTTATTGAAAATTAATGTGGTCCCCGTGTCTAGCTTTAATTGTGTTGCAGATGTAACTTGTTTCTTTCTCTTTCGTACATGTAACTTCTCAAATTATTTACTGCTGTAGGTGTCTCGTATCTTCCATCCAAATTTCTCTGCTAAATGGAGGCGATATCTGTATATTTTTCCTCTGAATGATGAAGAGAACATGGAGCCAGATTTTGCAATTTGGGAAGATGTAGAAAACTGTAGAGAACAGGAAAATTATGATAAAATAGAAGTCTTAAATGGTGAAGAATGCAAGAACGAGATTCATGATAATTTGGTCCTCGGTCCAAATGATGAGGAGCTTGTGTCACCTGATAAACCTAAAACGTTTAGTATAAGGAGGGTTAACCAACTACTTAGGAAACTAGAAGGGAAGTTATTGTCGTACAAGATGTTTGCACGTGACACCAAAGCATCAAGAAATGAGTGAGAATCATGAATTGTTCCAATTATTGCCTTTTCTTTTGGTAGTTATTCTAGCTTCTAATTTTAGTATCATAATTCTTTTTGTTTTTTTTTTTTTTGATTATGTTTATTTCTTTCTGCTCATAAAGATCCTTTTTAAAAAAAAAAATTGTCAATATGAGATTAATGTGCTTGCTTGAAGCTTTCTTTTTCCCAAACGTTTTCTTTTCAATGACAGGAAGATGTTCTAAACTTTCTGACATGTACTAATTGCAACTACCGTCCTTTTCAGAGGTCCACCTACGGAGTGTTTTGTTTACCATGCACGAGCAGCAGAAGCTAGATTACCTTGCTTGGTATCTTTCCTATCTGTAGTCCTCTGCATGCTCTCATCCTCCTTTTCTTTGATTATCCTTTTCCTTCTACATTTCTCTCCTATTGAAATCTATCTGTAGTGTGGTAACTGTAAAACAATATTCTATACTCTATAGCTCACATACACTGGAAAGAGGTAGAACAAGGTTATTTGGATTGTGTTTTTTACTGATTCAGAACGTAGAAAACAGAAGATGAATTTGTGGAAGAGTATGAACCGAGGAAACATAGAAGAAATTTACTTTGACATTATATCTTCAGGGTGAAGTATTTGAATAGCAAACTAGCTGTAGTATGTGCTTCATTCAATGAATATTCAAATTTTATTCACAACGGTTGCTCTTGCTCTCTCTTCTGTTCTCTTCTTGTTTGTTTTTATATCCTAATCTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGTGTCAGAAGAGGTTGTTTTCCTCATGTTTTTGAAGAGTTTTGTTTCTTAAATCGATAGCTTACTCTTTGTTCTCTTTTGGCTTGATTTTTCTTTATAGTGAAAGCATGTTTGCCATTCGTGTCAACATGCTATCAGCATTTTTCTCTATTGAACTAACTTTTGCTGGGCATCTTCTTAAATCTAATCTTTTTTTTTTTTCCCAACAAAATTAGTGACGTTAATGAATGCAGCTACTCCAAATTTGGGGATCTGACTTAGTGGTGTTGTTCTATTGACAGGACCAAGGAGTAGGAAGAAGTGTTATGTGCGTTGAGCTGGTTGCTAATCGCTTCTTGCGTAAGGTATCATAAAAGACGATATTACTGAACGTAAAATTGGTCTATTTAGATTTAGATAATCCTTTCTTCAACTGCTTAAACTCGAGAGTTAACATCTTAAACGTCAGCAAGGGAGTTTCCCCTTCGTTCGAGCTAAGTGTGTTGCAACCATTAAGGCCATATGTCCAAATGAAAAAATGACACTAATTATTGTCTTATGGTGAATCCTTGTTTCTTTAGCAAGTTCAACCAATTATAATATTCTATAAGTTTCCTCAACACATAAATATTGTAGGGTCGGGCAGGTTACCCGTGAGATTAGTTGAGGTGAGCTGGTCTAGAGACTCACGGATATTAATAATAATAATGACGACGATGATGAAATCTAATAATGATCCTTGCAGATGGTACGGGTGCTTGTTGCGACAGCAATTAGAGAGGCAGCTGCTGGTGCAGGAGAGGATGTGTTACTAGAGTTGATGGATGCCACATGTCGACGTGCCACTGCACCGCCTGCGCCTCCTGATGGCCTCAGTCTTGTTGATGTAGGATATGCAGAATTTGATTCACGAAATTGCTTCATTTTGGATGACTAA

mRNA sequence

ATGATGGTCGAATTACTCAGGCCCAGATCAGCTGGGTCTATTGGGCTTTCCCAGTTGGACCAGCATGGGCTTGCCCTCTGCTTGACTGCATCTACTTGGATTTACAATCTCGTCCCTGCTGCGACGGCCGCTGCAACTGCAAGATACGGTCGTCCGTTCTCAGGTGGGGCGGTGGTTATGATACCAAATCCAAGGGCTAGACTTGTATCACCATCAGGTGCCAGTTTTGGTTTCAAGATTCTGATGGAGAATCGAGCTCAGCAAAAAGACTATCTTCACTATAACCACACTGATCGCTGCAGAAATGCTAGATGGACTGCCAGCTTTGTGCTTGGTTGTTATGTAATCCAATCGGAATCTGAGAAGGCTGTTAATCAAGCTGTTGTTATGCATTTGGCAGGGAAAGCTACCAACTCATGTATGGAAGGCCGTGGCAAGAAGTGCTTGATTTTTACTTTCATGCTGTTAATGGAAGGTCTTGATCATGATGATGCTGAGACTCCAGCGATTTACAACACAGCAGAGTTAAACAGAGCTTCATTCAAAGATAAGGCTGGGAGGTGGGCAAGGGCAACATTTAAGATTGTTCTTTCCTACCATGGAAATTCCTTTGATGGGTGGCAAAAGCAGCCAGGCTTGAATACTGTTCAGGGCTTGGTAGAAAAATCTCTAGGGAAGTTTGTAGATGAGAAGAAAGCTCAGATGCTGAAAGATAGATGCTTACCAATAGAAGGATGTGCTGTGGTTGCTGGGCGAACTGACAAAGGGGTGACAGCTCTGCGACAAGTTTGCTCATTCTATACTTGGCGCAGCGATGTTAAACCTCAAGACATTGAAGATTCTATCAATAGTGCAGCTCCTGGAAAACTCAGAACATTATCTGTTTCTCAGGTGTCTCGTATCTTCCATCCAAATTTCTCTGCTAAATGGAGGCGATATCTGTATATTTTTCCTCTGAATGATGAAGAGAACATGGAGCCAGATTTTGCAATTTGGGAAGATGTAGAAAACTGTAGAGAACAGGAAAATTATGATAAAATAGAAGTCTTAAATGGTGAAGAATGCAAGAACGAGATTCATGATAATTTGGTCCTCGGTCCAAATGATGAGGAGCTTGTGTCACCTGATAAACCTAAAACGTTTAGTATAAGGAGGGTTAACCAACTACTTAGGAAACTAGAAGGGAAGTTATTGTCGTACAAGATGTTTGCACGTGACACCAAAGCATCAAGAAATGAAGGTCCACCTACGGAGTGTTTTGTTTACCATGCACGAGCAGCAGAAGCTAGATTACCTTGCTTGGACCAAGGAGTAGGAAGAAGTGTTATGTGCGTTGAGCTGGTTGCTAATCGCTTCTTGCCAATTAGAGAGGCAGCTGCTGGTGCAGGAGAGGATGTGTTACTAGAGTTGATGGATGCCACATGTCGACGTGCCACTGCACCGCCTGCGCCTCCTGATGGCCTCAGTCTTGTTGATGTAGGATATGCAGAATTTGATTCACGAAATTGCTTCATTTTGGATGACTAA

Coding sequence (CDS)

ATGATGGTCGAATTACTCAGGCCCAGATCAGCTGGGTCTATTGGGCTTTCCCAGTTGGACCAGCATGGGCTTGCCCTCTGCTTGACTGCATCTACTTGGATTTACAATCTCGTCCCTGCTGCGACGGCCGCTGCAACTGCAAGATACGGTCGTCCGTTCTCAGGTGGGGCGGTGGTTATGATACCAAATCCAAGGGCTAGACTTGTATCACCATCAGGTGCCAGTTTTGGTTTCAAGATTCTGATGGAGAATCGAGCTCAGCAAAAAGACTATCTTCACTATAACCACACTGATCGCTGCAGAAATGCTAGATGGACTGCCAGCTTTGTGCTTGGTTGTTATGTAATCCAATCGGAATCTGAGAAGGCTGTTAATCAAGCTGTTGTTATGCATTTGGCAGGGAAAGCTACCAACTCATGTATGGAAGGCCGTGGCAAGAAGTGCTTGATTTTTACTTTCATGCTGTTAATGGAAGGTCTTGATCATGATGATGCTGAGACTCCAGCGATTTACAACACAGCAGAGTTAAACAGAGCTTCATTCAAAGATAAGGCTGGGAGGTGGGCAAGGGCAACATTTAAGATTGTTCTTTCCTACCATGGAAATTCCTTTGATGGGTGGCAAAAGCAGCCAGGCTTGAATACTGTTCAGGGCTTGGTAGAAAAATCTCTAGGGAAGTTTGTAGATGAGAAGAAAGCTCAGATGCTGAAAGATAGATGCTTACCAATAGAAGGATGTGCTGTGGTTGCTGGGCGAACTGACAAAGGGGTGACAGCTCTGCGACAAGTTTGCTCATTCTATACTTGGCGCAGCGATGTTAAACCTCAAGACATTGAAGATTCTATCAATAGTGCAGCTCCTGGAAAACTCAGAACATTATCTGTTTCTCAGGTGTCTCGTATCTTCCATCCAAATTTCTCTGCTAAATGGAGGCGATATCTGTATATTTTTCCTCTGAATGATGAAGAGAACATGGAGCCAGATTTTGCAATTTGGGAAGATGTAGAAAACTGTAGAGAACAGGAAAATTATGATAAAATAGAAGTCTTAAATGGTGAAGAATGCAAGAACGAGATTCATGATAATTTGGTCCTCGGTCCAAATGATGAGGAGCTTGTGTCACCTGATAAACCTAAAACGTTTAGTATAAGGAGGGTTAACCAACTACTTAGGAAACTAGAAGGGAAGTTATTGTCGTACAAGATGTTTGCACGTGACACCAAAGCATCAAGAAATGAAGGTCCACCTACGGAGTGTTTTGTTTACCATGCACGAGCAGCAGAAGCTAGATTACCTTGCTTGGACCAAGGAGTAGGAAGAAGTGTTATGTGCGTTGAGCTGGTTGCTAATCGCTTCTTGCCAATTAGAGAGGCAGCTGCTGGTGCAGGAGAGGATGTGTTACTAGAGTTGATGGATGCCACATGTCGACGTGCCACTGCACCGCCTGCGCCTCCTGATGGCCTCAGTCTTGTTGATGTAGGATATGCAGAATTTGATTCACGAAATTGCTTCATTTTGGATGACTAA

Protein sequence

MMVELLRPRSAGSIGLSQLDQHGLALCLTASTWIYNLVPAATAAATARYGRPFSGGAVVMIPNPRARLVSPSGASFGFKILMENRAQQKDYLHYNHTDRCRNARWTASFVLGCYVIQSESEKAVNQAVVMHLAGKATNSCMEGRGKKCLIFTFMLLMEGLDHDDAETPAIYNTAELNRASFKDKAGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQMLKDRCLPIEGCAVVAGRTDKGVTALRQVCSFYTWRSDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDEENMEPDFAIWEDVENCREQENYDKIEVLNGEECKNEIHDNLVLGPNDEELVSPDKPKTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAAEARLPCLDQGVGRSVMCVELVANRFLPIREAAAGAGEDVLLELMDATCRRATAPPAPPDGLSLVDVGYAEFDSRNCFILDD
Homology
BLAST of CcUC06G124030 vs. NCBI nr
Match: XP_038878348.1 (tRNA pseudouridine synthase A [Benincasa hispida])

HSP 1 Score: 748.0 bits (1930), Expect = 5.2e-212
Identity = 377/459 (82.14%), Postives = 393/459 (85.62%), Query Frame = 0

Query: 60  MIPNPRARLVSPSGASFGFKILMENRAQQKDYLHYNHTDRCRNARWTASFVLGCYVIQSE 119
           MIPNPRARLVSPSGA FGFKILMENRAQQKDYLHYNHTDRCRNARWTA         +  
Sbjct: 1   MIPNPRARLVSPSGAGFGFKILMENRAQQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 60

Query: 120 SEKAVNQAVVMHLAGKATNSCMEGRGKKCLIFTFMLLMEGLDHDDAETPAIYNTAELNRA 179
            E       V+     A N    GR     +F   +   G DHDDAE P IYNTAELNR 
Sbjct: 61  QE-------VLDFYFHAVN----GRFSLSSLFGTEI---GHDHDDAEIPLIYNTAELNRV 120

Query: 180 SFKDKAGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQMLKDR 239
           S KDK GRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVD+KKAQMLKD+
Sbjct: 121 SVKDKTGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDDKKAQMLKDK 180

Query: 240 CLPIEGCAVVAGRTDKGVTALRQVCSFYTWRSDVKPQDIEDSINSAAPGKLRTLSVSQVS 299
           CLPIEGCAVVAGRTDKGVTALRQVCSFYTWR+DV+PQDIEDSIN AAPGKLRTLSVSQVS
Sbjct: 181 CLPIEGCAVVAGRTDKGVTALRQVCSFYTWRTDVQPQDIEDSINRAAPGKLRTLSVSQVS 240

Query: 300 RIFHPNFSAKWRRYLYIFPLNDEENMEPDFAIWEDVENCREQENYDKIEVLNGEECKNEI 359
           RIFHPNFSAKWRRYLYIFPLND ENMEPD+AIWEDVE+CR++ENY KIEVLNGEECK+EI
Sbjct: 241 RIFHPNFSAKWRRYLYIFPLNDGENMEPDYAIWEDVEDCRKEENYGKIEVLNGEECKDEI 300

Query: 360 HDNLVLGPNDEELVSPDKPKTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTEC 419
           HDNLV GPNDEELVS DKP+TFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTEC
Sbjct: 301 HDNLVFGPNDEELVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTEC 360

Query: 420 FVYHARAAEARLPCLDQGVGRSVMCVELVANRFL----------PIREAAAGAGEDVLLE 479
           FVYHARA EARLPCLDQGVGRSVMCVELVANRFL           IREAAAGAGED LLE
Sbjct: 361 FVYHARAVEARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLE 420

Query: 480 LMDATCRRATAPPAPPDGLSLVDVGYAEFDSRNCFILDD 509
           LMDATCRRATAPPAPPDGLSLVDVGY EFDS+NCFIL+D
Sbjct: 421 LMDATCRRATAPPAPPDGLSLVDVGYTEFDSQNCFILND 445

BLAST of CcUC06G124030 vs. NCBI nr
Match: XP_008453520.1 (PREDICTED: tRNA pseudouridine synthase A [Cucumis melo] >KAA0058161.1 tRNA pseudouridine synthase A [Cucumis melo var. makuwa])

HSP 1 Score: 710.7 bits (1833), Expect = 9.2e-201
Identity = 366/466 (78.54%), Postives = 384/466 (82.40%), Query Frame = 0

Query: 60  MIPNPRARLVSPSGASFGFKILMENRAQQKDYLHYNHTDRCRNARWTA----SFVLG--- 119
           MIPNPRARLVSPS A FG KILMENRAQQKDYLHYNHTDRCRNARWTA     F+ G   
Sbjct: 1   MIPNPRARLVSPSSAGFGLKILMENRAQQKDYLHYNHTDRCRNARWTARESFQFMYGRPW 60

Query: 120 CYVIQSESEKAVNQAVVMHLAGKATNSCMEGRGKKCLIFTFMLLMEGLDHDDAETPAIYN 179
             V+   S     +  +  L G  T  C                    DHDDAETP IYN
Sbjct: 61  QEVLDFYSHAVNGRLSLSSLLG--TEICR-------------------DHDDAETPLIYN 120

Query: 180 TAELNRASFKDKAGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKK 239
           +AELNRA+ KDK GRWARA FKIVLSYHGNSFDGWQKQPGLNTVQGLVE+SLGKFVDEKK
Sbjct: 121 SAELNRAAVKDKTGRWARANFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKK 180

Query: 240 AQMLKDRCLPIEGCAVVAGRTDKGVTALRQVCSFYTWRSDVKPQDIEDSINSAAPGKLRT 299
           AQMLKD+CLP+EGCAVVAGRTDKGVTALRQVCSFYTWR+DVKPQDIEDSINSAAPGKLRT
Sbjct: 181 AQMLKDKCLPLEGCAVVAGRTDKGVTALRQVCSFYTWRTDVKPQDIEDSINSAAPGKLRT 240

Query: 300 LSVSQVSRIFHPNFSAKWRRYLYIFPLNDEENMEPDFAIWEDVENCREQENYDKIEVLNG 359
           LSVSQVSRIFHPNFSAKWRRYLYI PLND ENMEPD AIWEDVEN RE+EN  KIE LNG
Sbjct: 241 LSVSQVSRIFHPNFSAKWRRYLYILPLNDGENMEPDSAIWEDVEN-REEENCVKIEALNG 300

Query: 360 EECKNEIHDNLVLGPNDEELVSPDKPKTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRN 419
           E+C + IHDNLVL PND+ELVS DKP+TFSIRRVNQLLRKLEGKLLSYKMFARDTKASRN
Sbjct: 301 EKCVDGIHDNLVLSPNDDELVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRN 360

Query: 420 EGPPTECFVYHARAAEARLPCLDQGVGRSVMCVELVANRFL----------PIREAAAGA 479
           EGPPTECFVYHARAAE RLPCL+QGVGRSVMCVELVANRFL           IREAAAGA
Sbjct: 361 EGPPTECFVYHARAAETRLPCLEQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGA 420

Query: 480 GEDVLLELMDATCRRATAPPAPPDGLSLVDVGYAEFDSRNCFILDD 509
           GED LLELM ATCRRATAPPAPPDGLSLVDVGY EFDS+ CFIL+D
Sbjct: 421 GEDALLELMVATCRRATAPPAPPDGLSLVDVGYTEFDSQICFILND 444

BLAST of CcUC06G124030 vs. NCBI nr
Match: XP_023006334.1 (uncharacterized protein LOC111499102 isoform X1 [Cucurbita maxima])

HSP 1 Score: 709.5 bits (1830), Expect = 2.0e-200
Identity = 375/501 (74.85%), Postives = 403/501 (80.44%), Query Frame = 0

Query: 26  LCLTA-STWIYNLVPAATAAATARYGRPFS-GGAVVMIPNPRARLVSPSGASFGF----- 85
           +CLTA STWIY +VPAA AAATA+Y RP   GGAVVM+PNPRARL+SPSGA FGF     
Sbjct: 7   ICLTATSTWIYIIVPAAPAAATAKYCRPLRLGGAVVMLPNPRARLLSPSGAGFGFGFGFG 66

Query: 86  -KILMENRAQQKDYLHYNHTDRCRNARWTASFVLGCYVIQSESEKAVNQAVVMHLAGKAT 145
            K LMENRAQ+KDYLHYNHTDRCRNARWT+      Y +  E         V+     A 
Sbjct: 67  IKCLMENRAQKKDYLHYNHTDRCRNARWTSR---ESYQLMYERP----WQEVLDFYSHAV 126

Query: 146 NSCMEGRGKKCLIFTFMLLMEGLDHDDAETPAIYNTAELNRASFKDKAGRWARATFKIVL 205
           N    GR    L  + +      DHDD E   + NTAELNRA  KD  GRWARATFKIVL
Sbjct: 127 N----GR----LSLSSLRTETVRDHDDTEIQPVCNTAELNRAPVKDGTGRWARATFKIVL 186

Query: 206 SYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQMLKDRCLPIEGCAVVAGRTDKGV 265
           SYHGNSFDGWQKQP LNTVQG+VEKSLGKFVDEKKAQ LKDRCLPIEGCAVVAGRTDKGV
Sbjct: 187 SYHGNSFDGWQKQPELNTVQGVVEKSLGKFVDEKKAQQLKDRCLPIEGCAVVAGRTDKGV 246

Query: 266 TALRQVCSFYTWRSDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRYLYIF 325
           TALRQVCSFYTWR+DVKPQDIE SIN+AAPGKLR LSVSQVSR+FHPNFSAKWRRYLYIF
Sbjct: 247 TALRQVCSFYTWRTDVKPQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFSAKWRRYLYIF 306

Query: 326 PLNDEENMEPDFAIWEDVENCREQENYDKIEVLNGEECKNEIHDNLVLGPNDEELVSPDK 385
           PL D ENMEP+FAI EDVEN RE+E  DKIEVL+G ECK+EI +NL+L  +DEE  S +K
Sbjct: 307 PLKDGENMEPNFAILEDVENSREEEYRDKIEVLHG-ECKDEILNNLILSQDDEEPASANK 366

Query: 386 PKTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAAEARLPCLDQG 445
           P+TFSI RVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARA EARLPCLDQG
Sbjct: 367 PQTFSIMRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLPCLDQG 426

Query: 446 VGRSVMCVELVANRFL----------PIREAAAGAGEDVLLELMDATCRRATAPPAPPDG 505
           VGRSVMCVELVANRFL           IREAAAGAGED LL+LMDATCRRATAPPAPPDG
Sbjct: 427 VGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPPAPPDG 486

Query: 506 LSLVDVGYAEFDSRNCFILDD 509
           LSLVDVGY EFD +NCFIL+D
Sbjct: 487 LSLVDVGYTEFDPQNCFILND 491

BLAST of CcUC06G124030 vs. NCBI nr
Match: KAG7021669.1 (truA, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 704.1 bits (1816), Expect = 8.6e-199
Identity = 369/497 (74.25%), Postives = 401/497 (80.68%), Query Frame = 0

Query: 26  LCLTA-STWIYNLVPAATAAATARYGRPFS-GGAVVMIPNPRARLVSPSGASFGF--KIL 85
           +CLTA STWIY +VPAA AAATA+Y RP   GGAVVMIPNPRA L+S SGA FGF  K L
Sbjct: 7   ICLTATSTWIYIIVPAAPAAATAKYSRPLRLGGAVVMIPNPRAILLSSSGAGFGFGIKCL 66

Query: 86  MENRAQQKDYLHYNHTDRCRNARWTASFVLGCYVIQSESEKAVNQAVVMHLAGKATNSCM 145
           MENRAQ+KDYLHYNHTDRCRNARWT+         Q   E+   + +  +      +  +
Sbjct: 67  MENRAQKKDYLHYNHTDRCRNARWTSR-----ESYQFMYERPWQEVLDFY------SHAV 126

Query: 146 EGRGKKCLIFTFMLLMEGLDHDDAETPAIYNTAELNRASFKDKAGRWARATFKIVLSYHG 205
            GR    L  + +      DHDD E   + NTAEL+RA  KD  GRWARATFKIVLSYHG
Sbjct: 127 NGR----LSLSSLGTETVRDHDDTEIQPVCNTAELDRAPVKDGTGRWARATFKIVLSYHG 186

Query: 206 NSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQMLKDRCLPIEGCAVVAGRTDKGVTALR 265
           NSFDGWQKQP LNTVQG+VEKSLGKFVDEKKAQ LKDRCLPIEGCAVVAGRTDKGVTALR
Sbjct: 187 NSFDGWQKQPDLNTVQGVVEKSLGKFVDEKKAQQLKDRCLPIEGCAVVAGRTDKGVTALR 246

Query: 266 QVCSFYTWRSDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLND 325
           QVCSFYTWR+DVKPQDIE SIN+AAPGKLR LSVSQVSR+FHPNFSAKWRRYLYIFPL D
Sbjct: 247 QVCSFYTWRTDVKPQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFSAKWRRYLYIFPLKD 306

Query: 326 EENMEPDFAIWEDVENCREQENYDKIEVLNGEECKNEIHDNLVLGPNDEELVSPDKPKTF 385
            E MEP+FAIWEDVEN RE+E+ DKIE L+G ECK+EI +NLVL  +DEE    +KP+TF
Sbjct: 307 GEKMEPNFAIWEDVENSREEEHRDKIEALHG-ECKDEILNNLVLSQDDEEPALANKPQTF 366

Query: 386 SIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAAEARLPCLDQGVGRS 445
           SI RVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARA EARLPCLDQGVGRS
Sbjct: 367 SIMRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLPCLDQGVGRS 426

Query: 446 VMCVELVANRFL----------PIREAAAGAGEDVLLELMDATCRRATAPPAPPDGLSLV 505
           VMCVELVANRFL           IREAAAGAGED LL+LMDATCRRATAPPAPPDGLSLV
Sbjct: 427 VMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPPAPPDGLSLV 486

Query: 506 DVGYAEFDSRNCFILDD 509
           DVGY EFD +NCFIL+D
Sbjct: 487 DVGYTEFDPQNCFILND 487

BLAST of CcUC06G124030 vs. NCBI nr
Match: XP_004137363.1 (uncharacterized protein LOC101205562 [Cucumis sativus] >KGN63901.1 hypothetical protein Csa_013558 [Cucumis sativus])

HSP 1 Score: 696.0 bits (1795), Expect = 2.3e-196
Identity = 357/466 (76.61%), Postives = 381/466 (81.76%), Query Frame = 0

Query: 60  MIPNPRARLVSPSGASFGFKILMENRAQQKDYLHYNHTDRCRNARWTA----SFVLG--- 119
           MIPNPRARLVSPS A FG KILMENRAQ+KDYLHYNHTDRCR+ARWTA     F+ G   
Sbjct: 1   MIPNPRARLVSPSSAGFGLKILMENRAQRKDYLHYNHTDRCRSARWTARESYQFMYGRPW 60

Query: 120 CYVIQSESEKAVNQAVVMHLAGKATNSCMEGRGKKCLIFTFMLLMEGLDHDDAETPAIYN 179
             V+   S     +  +  L G  T  C                    DHDDAETP IYN
Sbjct: 61  QEVLDFYSHAVNGRFSLSSLLG--TEICR-------------------DHDDAETPLIYN 120

Query: 180 TAELNRASFKDKAGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKK 239
           +AELNRA+ KDK GRWARATFKIVLSY GNSFDGWQKQPGLNTVQGLVE+SLGKFVDEKK
Sbjct: 121 SAELNRAAVKDKTGRWARATFKIVLSYDGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKK 180

Query: 240 AQMLKDRCLPIEGCAVVAGRTDKGVTALRQVCSFYTWRSDVKPQDIEDSINSAAPGKLRT 299
           AQMLKD+CLP+EGCA+VAGRTDKGVTALRQVCSFYTWR+DVKPQDIEDSINSAAPGKLRT
Sbjct: 181 AQMLKDKCLPLEGCALVAGRTDKGVTALRQVCSFYTWRTDVKPQDIEDSINSAAPGKLRT 240

Query: 300 LSVSQVSRIFHPNFSAKWRRYLYIFPLNDEENMEPDFAIWEDVENCREQENYDKIEVLNG 359
            SVSQVSRIFHPNFSAKWRRYLYI PLND ENMEPD   WE+VEN RE+EN+ KIE  NG
Sbjct: 241 SSVSQVSRIFHPNFSAKWRRYLYILPLNDGENMEPDSTTWENVEN-REEENFVKIEASNG 300

Query: 360 EECKNEIHDNLVLGPNDEELVSPDKPKTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRN 419
           E+C + IHDNLVL PND+ELVS DKP+TFSI RVNQLLRKLEGKLLSYKMFARDTKASRN
Sbjct: 301 EKCVDGIHDNLVLSPNDQELVSADKPQTFSITRVNQLLRKLEGKLLSYKMFARDTKASRN 360

Query: 420 EGPPTECFVYHARAAEARLPCLDQGVGRSVMCVELVANRFL----------PIREAAAGA 479
           EGPPTECFVYHARAAE RLPCL+QGVGRSVMCVELVANRFL           IREAAAGA
Sbjct: 361 EGPPTECFVYHARAAETRLPCLEQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGA 420

Query: 480 GEDVLLELMDATCRRATAPPAPPDGLSLVDVGYAEFDSRNCFILDD 509
           GED LLELMDATCRRATAPPAPPDGLSLVDVGY +FDS+ CFIL+D
Sbjct: 421 GEDALLELMDATCRRATAPPAPPDGLSLVDVGYTDFDSQICFILND 444

BLAST of CcUC06G124030 vs. ExPASy Swiss-Prot
Match: B5YDX5 (tRNA pseudouridine synthase A OS=Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) OX=309799 GN=truA PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 2.7e-14
Identity = 52/134 (38.81%), Postives = 72/134 (53.73%), Query Frame = 0

Query: 193 FKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQMLKDRCLPIEGCAVVAGR 252
           +K+ LSY G  F G+QKQPG  TVQG +EK L         ++L D  + + G    AGR
Sbjct: 4   WKVELSYIGKDFWGFQKQPGKRTVQGELEKVL---------KLLFDEDIKVIG----AGR 63

Query: 253 TDKGVTALRQVCSFYTWRS-DVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWR 312
           TD GV AL QV +F T  S +     +   +N   PG ++   V  V   FH  +SAK R
Sbjct: 64  TDAGVHALGQVVNFKTKESKNFSCDKLYKVLNKLLPGDIKIKKVEIVDDNFHARYSAKRR 123

Query: 313 RYLYIFPLNDEENM 326
            Y+Y+   N+E+N+
Sbjct: 124 WYIYVIYNNEEKNL 124

BLAST of CcUC06G124030 vs. ExPASy Swiss-Prot
Match: C0QZT8 (tRNA pseudouridine synthase A OS=Brachyspira hyodysenteriae (strain ATCC 49526 / WA1) OX=565034 GN=truA PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.0e-12
Identity = 46/123 (37.40%), Postives = 61/123 (49.59%), Query Frame = 0

Query: 194 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQMLKDRCLPIEGCAVVAGRT 253
           KI + Y G  F GWQ QP L TVQG + K++ K   EK         + I GC    GRT
Sbjct: 5   KITIQYDGTDFYGWQIQPNLRTVQGEIYKAVQKVYGEK---------ITIYGC----GRT 64

Query: 254 DKGVTALRQVCSFYTWRSDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRY 313
           D GV AL QV +F   +  V    +  ++NS     LR +   ++   F+   SA +R Y
Sbjct: 65  DAGVHALGQVANFRVPKMLVPINKVHIALNSYLDRDLRIIKAEEMPDSFNARASATFREY 114

Query: 314 LYI 317
           LYI
Sbjct: 125 LYI 114

BLAST of CcUC06G124030 vs. ExPASy Swiss-Prot
Match: B8E1G5 (tRNA pseudouridine synthase A OS=Dictyoglomus turgidum (strain DSM 6724 / Z-1310) OX=515635 GN=truA PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.6e-12
Identity = 52/150 (34.67%), Postives = 72/150 (48.00%), Query Frame = 0

Query: 193 FKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQMLKDRCLPIEGCAVVAGR 252
           +K+ +SY G  F G+QKQPG  T+QG +EK L    DE             E   + AGR
Sbjct: 4   WKLEISYIGKDFYGFQKQPGKRTIQGELEKVLSFLFDE-------------EIRVIGAGR 63

Query: 253 TDKGVTALRQVCSFYT-WRSDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWR 312
           TD GV AL QV +F +          +   +N   P  ++   V  V   FH  +SAK R
Sbjct: 64  TDTGVHALGQVVNFKSRGHKGFSCDKLHKVLNRLLPEDIKIKKVEIVDDSFHARYSAKRR 123

Query: 313 RYLYIFPLNDEENM-EPDFAIWEDVENCRE 341
            Y+Y+   NDE ++   D++ W   E  RE
Sbjct: 124 WYIYVVYNNDERDLFLKDYSWWISREINRE 140

BLAST of CcUC06G124030 vs. ExPASy Swiss-Prot
Match: Q01WL4 (tRNA pseudouridine synthase A OS=Solibacter usitatus (strain Ellin6076) OX=234267 GN=truA PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 3.3e-12
Identity = 41/122 (33.61%), Postives = 66/122 (54.10%), Query Frame = 0

Query: 194 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQMLKDRCLPIEGCAVVAGRT 253
           +I L+Y G  F GWQ QPGL T+QG++E+ +G          ++ + + + G    +GRT
Sbjct: 5   RIYLAYDGGPFHGWQVQPGLATIQGILEEIVGG---------MEGKPVHVAG----SGRT 64

Query: 254 DKGVTALRQVCSFYTWRSDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRY 313
           D GV AL QV +F +  + +  +++  ++N   P  +R L   +V   FHP F A  + Y
Sbjct: 65  DAGVHALAQVAAF-SIENPIPVENLRRAVNRLLPPAIRILCAEEVQADFHPRFDAVAKTY 112

Query: 314 LY 316
            Y
Sbjct: 125 EY 112

BLAST of CcUC06G124030 vs. ExPASy Swiss-Prot
Match: B0SC03 (tRNA pseudouridine synthase A OS=Leptospira biflexa serovar Patoc (strain Patoc 1 / Ames) OX=355278 GN=truA PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 4.4e-12
Identity = 53/164 (32.32%), Postives = 77/164 (46.95%), Query Frame = 0

Query: 193 FKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQMLKDRCLPIEGCAVVAGR 252
           + +++ Y G  F+GWQKQ  L TVQ  +E +LG  +    A  L            VAGR
Sbjct: 4   YALLVEYDGTHFNGWQKQKNLPTVQSSIESALGIILRRNPASRLS-----------VAGR 63

Query: 253 TDKGVTALRQVCSFYTWRSDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRR 312
           TD GV AL  VC+F T         +  S+N+  P  +   +V +V   FH  FS   R 
Sbjct: 64  TDTGVHALGMVCNFKTEHPIPNFHKLLVSLNALTPTGVSVKNVVEVPSDFHARFSCTGRE 123

Query: 313 YLY-IFPLNDEENMEPDFAIWEDVENCREQENYDK-IEVLNGEE 355
           Y+Y ++    E +     A W  V+   + E   K ++VL GE+
Sbjct: 124 YIYKLYYSKYESSFVEGRAFW--VKGHIDWERVKKQLQVLVGEK 154

BLAST of CcUC06G124030 vs. ExPASy TrEMBL
Match: A0A5A7UXE7 (tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004790 PE=3 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 4.4e-201
Identity = 366/466 (78.54%), Postives = 384/466 (82.40%), Query Frame = 0

Query: 60  MIPNPRARLVSPSGASFGFKILMENRAQQKDYLHYNHTDRCRNARWTA----SFVLG--- 119
           MIPNPRARLVSPS A FG KILMENRAQQKDYLHYNHTDRCRNARWTA     F+ G   
Sbjct: 1   MIPNPRARLVSPSSAGFGLKILMENRAQQKDYLHYNHTDRCRNARWTARESFQFMYGRPW 60

Query: 120 CYVIQSESEKAVNQAVVMHLAGKATNSCMEGRGKKCLIFTFMLLMEGLDHDDAETPAIYN 179
             V+   S     +  +  L G  T  C                    DHDDAETP IYN
Sbjct: 61  QEVLDFYSHAVNGRLSLSSLLG--TEICR-------------------DHDDAETPLIYN 120

Query: 180 TAELNRASFKDKAGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKK 239
           +AELNRA+ KDK GRWARA FKIVLSYHGNSFDGWQKQPGLNTVQGLVE+SLGKFVDEKK
Sbjct: 121 SAELNRAAVKDKTGRWARANFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKK 180

Query: 240 AQMLKDRCLPIEGCAVVAGRTDKGVTALRQVCSFYTWRSDVKPQDIEDSINSAAPGKLRT 299
           AQMLKD+CLP+EGCAVVAGRTDKGVTALRQVCSFYTWR+DVKPQDIEDSINSAAPGKLRT
Sbjct: 181 AQMLKDKCLPLEGCAVVAGRTDKGVTALRQVCSFYTWRTDVKPQDIEDSINSAAPGKLRT 240

Query: 300 LSVSQVSRIFHPNFSAKWRRYLYIFPLNDEENMEPDFAIWEDVENCREQENYDKIEVLNG 359
           LSVSQVSRIFHPNFSAKWRRYLYI PLND ENMEPD AIWEDVEN RE+EN  KIE LNG
Sbjct: 241 LSVSQVSRIFHPNFSAKWRRYLYILPLNDGENMEPDSAIWEDVEN-REEENCVKIEALNG 300

Query: 360 EECKNEIHDNLVLGPNDEELVSPDKPKTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRN 419
           E+C + IHDNLVL PND+ELVS DKP+TFSIRRVNQLLRKLEGKLLSYKMFARDTKASRN
Sbjct: 301 EKCVDGIHDNLVLSPNDDELVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRN 360

Query: 420 EGPPTECFVYHARAAEARLPCLDQGVGRSVMCVELVANRFL----------PIREAAAGA 479
           EGPPTECFVYHARAAE RLPCL+QGVGRSVMCVELVANRFL           IREAAAGA
Sbjct: 361 EGPPTECFVYHARAAETRLPCLEQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGA 420

Query: 480 GEDVLLELMDATCRRATAPPAPPDGLSLVDVGYAEFDSRNCFILDD 509
           GED LLELM ATCRRATAPPAPPDGLSLVDVGY EFDS+ CFIL+D
Sbjct: 421 GEDALLELMVATCRRATAPPAPPDGLSLVDVGYTEFDSQICFILND 444

BLAST of CcUC06G124030 vs. ExPASy TrEMBL
Match: A0A1S3BWI6 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103494203 PE=3 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 4.4e-201
Identity = 366/466 (78.54%), Postives = 384/466 (82.40%), Query Frame = 0

Query: 60  MIPNPRARLVSPSGASFGFKILMENRAQQKDYLHYNHTDRCRNARWTA----SFVLG--- 119
           MIPNPRARLVSPS A FG KILMENRAQQKDYLHYNHTDRCRNARWTA     F+ G   
Sbjct: 1   MIPNPRARLVSPSSAGFGLKILMENRAQQKDYLHYNHTDRCRNARWTARESFQFMYGRPW 60

Query: 120 CYVIQSESEKAVNQAVVMHLAGKATNSCMEGRGKKCLIFTFMLLMEGLDHDDAETPAIYN 179
             V+   S     +  +  L G  T  C                    DHDDAETP IYN
Sbjct: 61  QEVLDFYSHAVNGRLSLSSLLG--TEICR-------------------DHDDAETPLIYN 120

Query: 180 TAELNRASFKDKAGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKK 239
           +AELNRA+ KDK GRWARA FKIVLSYHGNSFDGWQKQPGLNTVQGLVE+SLGKFVDEKK
Sbjct: 121 SAELNRAAVKDKTGRWARANFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKK 180

Query: 240 AQMLKDRCLPIEGCAVVAGRTDKGVTALRQVCSFYTWRSDVKPQDIEDSINSAAPGKLRT 299
           AQMLKD+CLP+EGCAVVAGRTDKGVTALRQVCSFYTWR+DVKPQDIEDSINSAAPGKLRT
Sbjct: 181 AQMLKDKCLPLEGCAVVAGRTDKGVTALRQVCSFYTWRTDVKPQDIEDSINSAAPGKLRT 240

Query: 300 LSVSQVSRIFHPNFSAKWRRYLYIFPLNDEENMEPDFAIWEDVENCREQENYDKIEVLNG 359
           LSVSQVSRIFHPNFSAKWRRYLYI PLND ENMEPD AIWEDVEN RE+EN  KIE LNG
Sbjct: 241 LSVSQVSRIFHPNFSAKWRRYLYILPLNDGENMEPDSAIWEDVEN-REEENCVKIEALNG 300

Query: 360 EECKNEIHDNLVLGPNDEELVSPDKPKTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRN 419
           E+C + IHDNLVL PND+ELVS DKP+TFSIRRVNQLLRKLEGKLLSYKMFARDTKASRN
Sbjct: 301 EKCVDGIHDNLVLSPNDDELVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRN 360

Query: 420 EGPPTECFVYHARAAEARLPCLDQGVGRSVMCVELVANRFL----------PIREAAAGA 479
           EGPPTECFVYHARAAE RLPCL+QGVGRSVMCVELVANRFL           IREAAAGA
Sbjct: 361 EGPPTECFVYHARAAETRLPCLEQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGA 420

Query: 480 GEDVLLELMDATCRRATAPPAPPDGLSLVDVGYAEFDSRNCFILDD 509
           GED LLELM ATCRRATAPPAPPDGLSLVDVGY EFDS+ CFIL+D
Sbjct: 421 GEDALLELMVATCRRATAPPAPPDGLSLVDVGYTEFDSQICFILND 444

BLAST of CcUC06G124030 vs. ExPASy TrEMBL
Match: A0A6J1L1W3 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499102 PE=3 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 9.9e-201
Identity = 375/501 (74.85%), Postives = 403/501 (80.44%), Query Frame = 0

Query: 26  LCLTA-STWIYNLVPAATAAATARYGRPFS-GGAVVMIPNPRARLVSPSGASFGF----- 85
           +CLTA STWIY +VPAA AAATA+Y RP   GGAVVM+PNPRARL+SPSGA FGF     
Sbjct: 7   ICLTATSTWIYIIVPAAPAAATAKYCRPLRLGGAVVMLPNPRARLLSPSGAGFGFGFGFG 66

Query: 86  -KILMENRAQQKDYLHYNHTDRCRNARWTASFVLGCYVIQSESEKAVNQAVVMHLAGKAT 145
            K LMENRAQ+KDYLHYNHTDRCRNARWT+      Y +  E         V+     A 
Sbjct: 67  IKCLMENRAQKKDYLHYNHTDRCRNARWTSR---ESYQLMYERP----WQEVLDFYSHAV 126

Query: 146 NSCMEGRGKKCLIFTFMLLMEGLDHDDAETPAIYNTAELNRASFKDKAGRWARATFKIVL 205
           N    GR    L  + +      DHDD E   + NTAELNRA  KD  GRWARATFKIVL
Sbjct: 127 N----GR----LSLSSLRTETVRDHDDTEIQPVCNTAELNRAPVKDGTGRWARATFKIVL 186

Query: 206 SYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQMLKDRCLPIEGCAVVAGRTDKGV 265
           SYHGNSFDGWQKQP LNTVQG+VEKSLGKFVDEKKAQ LKDRCLPIEGCAVVAGRTDKGV
Sbjct: 187 SYHGNSFDGWQKQPELNTVQGVVEKSLGKFVDEKKAQQLKDRCLPIEGCAVVAGRTDKGV 246

Query: 266 TALRQVCSFYTWRSDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRYLYIF 325
           TALRQVCSFYTWR+DVKPQDIE SIN+AAPGKLR LSVSQVSR+FHPNFSAKWRRYLYIF
Sbjct: 247 TALRQVCSFYTWRTDVKPQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFSAKWRRYLYIF 306

Query: 326 PLNDEENMEPDFAIWEDVENCREQENYDKIEVLNGEECKNEIHDNLVLGPNDEELVSPDK 385
           PL D ENMEP+FAI EDVEN RE+E  DKIEVL+G ECK+EI +NL+L  +DEE  S +K
Sbjct: 307 PLKDGENMEPNFAILEDVENSREEEYRDKIEVLHG-ECKDEILNNLILSQDDEEPASANK 366

Query: 386 PKTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAAEARLPCLDQG 445
           P+TFSI RVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARA EARLPCLDQG
Sbjct: 367 PQTFSIMRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLPCLDQG 426

Query: 446 VGRSVMCVELVANRFL----------PIREAAAGAGEDVLLELMDATCRRATAPPAPPDG 505
           VGRSVMCVELVANRFL           IREAAAGAGED LL+LMDATCRRATAPPAPPDG
Sbjct: 427 VGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPPAPPDG 486

Query: 506 LSLVDVGYAEFDSRNCFILDD 509
           LSLVDVGY EFD +NCFIL+D
Sbjct: 487 LSLVDVGYTEFDPQNCFILND 491

BLAST of CcUC06G124030 vs. ExPASy TrEMBL
Match: A0A0A0LV59 (tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G025830 PE=3 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 1.1e-196
Identity = 357/466 (76.61%), Postives = 381/466 (81.76%), Query Frame = 0

Query: 60  MIPNPRARLVSPSGASFGFKILMENRAQQKDYLHYNHTDRCRNARWTA----SFVLG--- 119
           MIPNPRARLVSPS A FG KILMENRAQ+KDYLHYNHTDRCR+ARWTA     F+ G   
Sbjct: 1   MIPNPRARLVSPSSAGFGLKILMENRAQRKDYLHYNHTDRCRSARWTARESYQFMYGRPW 60

Query: 120 CYVIQSESEKAVNQAVVMHLAGKATNSCMEGRGKKCLIFTFMLLMEGLDHDDAETPAIYN 179
             V+   S     +  +  L G  T  C                    DHDDAETP IYN
Sbjct: 61  QEVLDFYSHAVNGRFSLSSLLG--TEICR-------------------DHDDAETPLIYN 120

Query: 180 TAELNRASFKDKAGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKK 239
           +AELNRA+ KDK GRWARATFKIVLSY GNSFDGWQKQPGLNTVQGLVE+SLGKFVDEKK
Sbjct: 121 SAELNRAAVKDKTGRWARATFKIVLSYDGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKK 180

Query: 240 AQMLKDRCLPIEGCAVVAGRTDKGVTALRQVCSFYTWRSDVKPQDIEDSINSAAPGKLRT 299
           AQMLKD+CLP+EGCA+VAGRTDKGVTALRQVCSFYTWR+DVKPQDIEDSINSAAPGKLRT
Sbjct: 181 AQMLKDKCLPLEGCALVAGRTDKGVTALRQVCSFYTWRTDVKPQDIEDSINSAAPGKLRT 240

Query: 300 LSVSQVSRIFHPNFSAKWRRYLYIFPLNDEENMEPDFAIWEDVENCREQENYDKIEVLNG 359
            SVSQVSRIFHPNFSAKWRRYLYI PLND ENMEPD   WE+VEN RE+EN+ KIE  NG
Sbjct: 241 SSVSQVSRIFHPNFSAKWRRYLYILPLNDGENMEPDSTTWENVEN-REEENFVKIEASNG 300

Query: 360 EECKNEIHDNLVLGPNDEELVSPDKPKTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRN 419
           E+C + IHDNLVL PND+ELVS DKP+TFSI RVNQLLRKLEGKLLSYKMFARDTKASRN
Sbjct: 301 EKCVDGIHDNLVLSPNDQELVSADKPQTFSITRVNQLLRKLEGKLLSYKMFARDTKASRN 360

Query: 420 EGPPTECFVYHARAAEARLPCLDQGVGRSVMCVELVANRFL----------PIREAAAGA 479
           EGPPTECFVYHARAAE RLPCL+QGVGRSVMCVELVANRFL           IREAAAGA
Sbjct: 361 EGPPTECFVYHARAAETRLPCLEQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGA 420

Query: 480 GEDVLLELMDATCRRATAPPAPPDGLSLVDVGYAEFDSRNCFILDD 509
           GED LLELMDATCRRATAPPAPPDGLSLVDVGY +FDS+ CFIL+D
Sbjct: 421 GEDALLELMDATCRRATAPPAPPDGLSLVDVGYTDFDSQICFILND 444

BLAST of CcUC06G124030 vs. ExPASy TrEMBL
Match: A0A6J1L1X2 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499102 PE=3 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 9.9e-193
Identity = 366/493 (74.24%), Postives = 392/493 (79.51%), Query Frame = 0

Query: 26  LCLTA-STWIYNLVPAATAAATARYGRPFS-GGAVVMIPNPRARLVSPSGASFGF----- 85
           +CLTA STWIY +VPAA AAATA+Y RP   GGAVVM+PNPRARL+SPSGA FGF     
Sbjct: 7   ICLTATSTWIYIIVPAAPAAATAKYCRPLRLGGAVVMLPNPRARLLSPSGAGFGFGFGFG 66

Query: 86  -KILMENRAQQKDYLHYNHTDRCRNARWTASFVLGCYVIQSESEKAVNQAVVMHLAGKAT 145
            K LMENRAQ+KDYLHYNHTDRCRNARWT+      Y +  E         V+     A 
Sbjct: 67  IKCLMENRAQKKDYLHYNHTDRCRNARWTSR---ESYQLMYERP----WQEVLDFYSHAV 126

Query: 146 NSCMEGRGKKCLIFTFMLLMEGLDHDDAETPAIYNTAELNRASFKDKAGRWARATFKIVL 205
           N    GR    L  + +      DHDD E   + NTAELNRA  KD  GRWARATFKIVL
Sbjct: 127 N----GR----LSLSSLRTETVRDHDDTEIQPVCNTAELNRAPVKDGTGRWARATFKIVL 186

Query: 206 SYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQMLKDRCLPIEGCAVVAGRTDKGV 265
           SYHGNSFDGWQKQP LNTVQG+VEKSLGKFVDEKKAQ LKDRCLPIEGCAVVAGRTDKGV
Sbjct: 187 SYHGNSFDGWQKQPELNTVQGVVEKSLGKFVDEKKAQQLKDRCLPIEGCAVVAGRTDKGV 246

Query: 266 TALRQVCSFYTWRSDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRYLYIF 325
           TALRQVCSFYTWR+DVKPQDIE SIN+AAPGKLR LSVSQVSR+FHPNFSAKWRRYLYIF
Sbjct: 247 TALRQVCSFYTWRTDVKPQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFSAKWRRYLYIF 306

Query: 326 PLNDEENMEPDFAIWEDVENCREQENYDKIEVLNGEECKNEIHDNLVLGPNDEELVSPDK 385
           PL D ENMEP+FAI EDVEN RE+E  DKIEVL+G ECK+EI +NL+L  +DEE  S +K
Sbjct: 307 PLKDGENMEPNFAILEDVENSREEEYRDKIEVLHG-ECKDEILNNLILSQDDEEPASANK 366

Query: 386 PKTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAAEARLPCLDQG 445
           P+TFSI RVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARA EARLPCLDQG
Sbjct: 367 PQTFSIMRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLPCLDQG 426

Query: 446 VGRSVMCVELVANRFL----------PIREAAAGAGEDVLLELMDATCRRATAPPAPPDG 501
           VGRSVMCVELVANRFL           IREAAAGAGED LL+LMDATCRRATAPPAPPDG
Sbjct: 427 VGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPPAPPDG 483

BLAST of CcUC06G124030 vs. TAIR 10
Match: AT5G35400.1 (Pseudouridine synthase family protein )

HSP 1 Score: 412.9 bits (1060), Expect = 3.7e-115
Identity = 226/429 (52.68%), Postives = 284/429 (66.20%), Query Frame = 0

Query: 91  YLHYNHTDRCRNARWTASFVLGCYVIQSESEKAVNQAVVMHLAGKATNSCMEGRGKKCLI 150
           Y+HY+H D C  ARW A           E+ + +      H+    +N+ +EG      +
Sbjct: 6   YVHYHHADACSKARWNA----------RETFRFMYDRPWQHVLDFYSNA-VEG---NLSV 65

Query: 151 FTFMLLMEGLDHDDAETPAIYNTAELNRASFKD-KAGRWARATFKIVLSYHGNSFDGWQK 210
            +     + L HDD  T  +   +E   ++ KD ++GRW R  FKIVLSY+G SFDGWQK
Sbjct: 66  LSLFQPKKSLVHDDGITEEMLLESEPETSTRKDGRSGRWERVNFKIVLSYNGASFDGWQK 125

Query: 211 QPGLNTVQGLVEKSLGKFVDEKKAQMLKDRCLPIEGCAVVAGRTDKGVTALRQVCSFYTW 270
           QP L+TVQG+VEKSLG FVDE+KAQ+LK +C P+EG  +VAGRTDKGV+AL QVCSFYTW
Sbjct: 126 QPDLHTVQGVVEKSLGGFVDERKAQLLKKKCKPLEGRVLVAGRTDKGVSALNQVCSFYTW 185

Query: 271 RSDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDEENMEPDF 330
           R D++P  IED+IN  A GKLR +S+S+VSR FHPNFSAKWRRYLYIFPL+D E +    
Sbjct: 186 RKDIEPIAIEDAINKDASGKLRVVSISKVSRSFHPNFSAKWRRYLYIFPLDDAEPLRKSG 245

Query: 331 AIWEDV---ENCREQENYDKIEVLNGEECKNEIHDNLVLGPNDEELVSPDKPKTFSIRRV 390
              E+    EN ++Q N    E +  E   +E  D L +      L   +KP  FS+ +V
Sbjct: 246 ENHENFIFDENKKKQRNGLLSEEIAEEVIMSE-DDELEIEETSNALEVVEKPSDFSVSKV 305

Query: 391 NQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAAEARLPCLDQGVGRSVMCVE 450
           +QLL+ L+GK+LSYKMFARDTKA+RNEGPPTECF+YHARAAE RL   D   GR VMCVE
Sbjct: 306 DQLLQHLQGKVLSYKMFARDTKAARNEGPPTECFMYHARAAEIRLSSSDCVEGRRVMCVE 365

Query: 451 LVANRFL----------PIREAAAGAGEDVLLELMDATCRRATAPPAPPDGLSLVDVGYA 506
           LVANRFL           IREAAAGA +D LL+L++A+CRRATAPPAP +GL L DVGY 
Sbjct: 366 LVANRFLRKMVRVLVATSIREAAAGAEDDALLKLLEASCRRATAPPAPSEGLCLFDVGYT 419

BLAST of CcUC06G124030 vs. TAIR 10
Match: AT5G35400.2 (Pseudouridine synthase family protein )

HSP 1 Score: 389.4 bits (999), Expect = 4.4e-108
Identity = 226/480 (47.08%), Postives = 284/480 (59.17%), Query Frame = 0

Query: 91  YLHYNHTDRCRNARWTASFVLGCYVIQSESEKAVNQAVVMHLAGKATNSCMEGRGKKCLI 150
           Y+HY+H D C  ARW A           E+ + +      H+    +N+ +EG      +
Sbjct: 6   YVHYHHADACSKARWNA----------RETFRFMYDRPWQHVLDFYSNA-VEG---NLSV 65

Query: 151 FTFMLLMEGLDHDDAETPAIYNTAELNRASFKD-KAGRWARATFKIVLSYHGNSFDGWQK 210
            +     + L HDD  T  +   +E   ++ KD ++GRW R  FKIVLSY+G SFDGWQK
Sbjct: 66  LSLFQPKKSLVHDDGITEEMLLESEPETSTRKDGRSGRWERVNFKIVLSYNGASFDGWQK 125

Query: 211 QPGLNTVQGLVEKSLGKFVDEKKAQMLKDRCLPIEGCAVVAGRTDKGVTALRQVCSFYTW 270
           QP L+TVQG+VEKSLG FVDE+KAQ+LK +C P+EG  +VAGRTDKGV+AL QVCSFYTW
Sbjct: 126 QPDLHTVQGVVEKSLGGFVDERKAQLLKKKCKPLEGRVLVAGRTDKGVSALNQVCSFYTW 185

Query: 271 RSDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRYLYIFPLNDEENMEPDF 330
           R D++P  IED+IN  A GKLR +S+S+VSR FHPNFSAKWRRYLYIFPL+D E +    
Sbjct: 186 RKDIEPIAIEDAINKDASGKLRVVSISKVSRSFHPNFSAKWRRYLYIFPLDDAEPLRKSG 245

Query: 331 AIWEDV---ENCREQENYDKIEVLNGEECKNEIHDNLVLGPNDEELVSPDKPKTFSIRRV 390
              E+    EN ++Q N    E +  E   +E  D L +      L   +KP  FS+ +V
Sbjct: 246 ENHENFIFDENKKKQRNGLLSEEIAEEVIMSE-DDELEIEETSNALEVVEKPSDFSVSKV 305

Query: 391 NQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAAEARLP-------------- 450
           +QLL+ L+GK+LSYKMFARDTKA+RNEGPPTECF+YHARAAE RL               
Sbjct: 306 DQLLQHLQGKVLSYKMFARDTKAARNEGPPTECFMYHARAAEIRLSSSVVHFVAWVFGGS 365

Query: 451 -------------------------------------CLDQGVGRSVMCVELVANRFL-- 506
                                                  D   GR VMCVELVANRFL  
Sbjct: 366 IVLVILATEGSTLDLTDTKLYIASWFLIKRSCEFLFIASDCVEGRRVMCVELVANRFLRK 425

BLAST of CcUC06G124030 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 62.8 bits (151), Expect = 9.3e-10
Identity = 43/139 (30.94%), Postives = 66/139 (47.48%), Query Frame = 0

Query: 184 KAGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSL---GKFVDEKKAQMLKDRC 243
           +A ++ R    IV ++ G  + G QK PG  T++G +E++L   G   D        DR 
Sbjct: 51  RAPKYRRRKVAIVFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPD-------ADRN 110

Query: 244 LPIEGCAVVAGRTDKGVTALRQVCSFYTWRSDVKPQDIEDSINSAAPGKLRTLSVSQVSR 303
            P       + RTDKGV+A+ QV S    R  V P    + +NS  P ++R     +V+ 
Sbjct: 111 KPRNYEWARSARTDKGVSAVGQVVS---GRFYVDPPGFVERLNSKLPDQIRVFGYKRVAP 170

Query: 304 IFHPNFSAKWRRYLYIFPL 320
            F        RRY+Y+ P+
Sbjct: 171 SFSSKKFCDRRRYVYLIPV 179

BLAST of CcUC06G124030 vs. TAIR 10
Match: AT3G06950.1 (Pseudouridine synthase family protein )

HSP 1 Score: 60.1 bits (144), Expect = 6.0e-09
Identity = 58/214 (27.10%), Postives = 93/214 (43.46%), Query Frame = 0

Query: 193 FKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQMLKDRCLPIEGCAVVAGR 252
           +++V++Y G  F GWQ Q    T+Q ++EK+L +  +  + ++            + AGR
Sbjct: 43  WRLVIAYDGTRFAGWQYQESPPTIQSMLEKALIQITELGRKELQ----------LIGAGR 102

Query: 253 TDKGVTALRQVCSFYTWRSDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRR 312
           TD GV A  QV  F T  +         ++N   P  +R   +S     FH  FSA  + 
Sbjct: 103 TDAGVHAWGQVAHFVTPFNYTSLDSFHAALNGLLPKDIRVRELSAAVPEFHARFSASSKV 162

Query: 313 YLYIFPLNDEENMEPDFAIWEDVENCREQENYDKI-EVLNGEECKNEIHDNLVLGPNDEE 372
           Y Y   + ++  M+P    W    +C  + N  K+ E  N    K   HD         E
Sbjct: 163 YRY--QIYNDTFMDPFQRHW--AYHCAYKLNASKMREAANLFVGK---HDFSAFANATRE 222

Query: 373 LVSPDKPKT---FSIRRVNQLLR-KLEGKLLSYK 402
              PD  KT   F + ++  LL+ ++EG    Y+
Sbjct: 223 DGVPDPLKTISRFDVIQMGSLLQLEVEGSGFLYR 239

BLAST of CcUC06G124030 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 55.8 bits (133), Expect = 1.1e-07
Identity = 37/125 (29.60%), Postives = 59/125 (47.20%), Query Frame = 0

Query: 195 IVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQMLKDRCLPIEGCAVVAGRTD 254
           I+ ++ G  + G QK PG  T++G +E++L       ++   K +       A    RTD
Sbjct: 26  IIFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA----RTD 85

Query: 255 KGVTALRQVCSFYTWRSDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRYL 314
           KGV+A+ QV S    R  V P    + +NS  P ++R      V+  F        RRY+
Sbjct: 86  KGVSAVGQVVS---GRFIVDPLGFVNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYV 143

Query: 315 YIFPL 320
           Y+ P+
Sbjct: 146 YLLPV 143

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878348.15.2e-21282.14tRNA pseudouridine synthase A [Benincasa hispida][more]
XP_008453520.19.2e-20178.54PREDICTED: tRNA pseudouridine synthase A [Cucumis melo] >KAA0058161.1 tRNA pseud... [more]
XP_023006334.12.0e-20074.85uncharacterized protein LOC111499102 isoform X1 [Cucurbita maxima][more]
KAG7021669.18.6e-19974.25truA, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_004137363.12.3e-19676.61uncharacterized protein LOC101205562 [Cucumis sativus] >KGN63901.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
B5YDX52.7e-1438.81tRNA pseudouridine synthase A OS=Dictyoglomus thermophilum (strain ATCC 35947 / ... [more]
C0QZT82.0e-1237.40tRNA pseudouridine synthase A OS=Brachyspira hyodysenteriae (strain ATCC 49526 /... [more]
B8E1G52.6e-1234.67tRNA pseudouridine synthase A OS=Dictyoglomus turgidum (strain DSM 6724 / Z-1310... [more]
Q01WL43.3e-1233.61tRNA pseudouridine synthase A OS=Solibacter usitatus (strain Ellin6076) OX=23426... [more]
B0SC034.4e-1232.32tRNA pseudouridine synthase A OS=Leptospira biflexa serovar Patoc (strain Patoc ... [more]
Match NameE-valueIdentityDescription
A0A5A7UXE74.4e-20178.54tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A1S3BWI64.4e-20178.54tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103494203 PE=3 SV=1[more]
A0A6J1L1W39.9e-20174.85tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499102 PE=3 SV=... [more]
A0A0A0LV591.1e-19676.61tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G025830 PE=3 SV=1[more]
A0A6J1L1X29.9e-19374.24tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499102 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT5G35400.13.7e-11552.68Pseudouridine synthase family protein [more]
AT5G35400.24.4e-10847.08Pseudouridine synthase family protein [more]
AT1G20370.19.3e-1030.94Pseudouridine synthase family protein [more]
AT3G06950.16.0e-0927.10Pseudouridine synthase family protein [more]
AT1G76120.11.1e-0729.60Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020094Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminalGENE3D3.30.70.580coord: 190..308
e-value: 8.3E-24
score: 85.9
IPR001406Pseudouridine synthase I, TruAPANTHERPTHR11142PSEUDOURIDYLATE SYNTHASEcoord: 147..505
NoneNo IPR availablePANTHERPTHR11142:SF10TRNA PSEUDOURIDINE SYNTHASEcoord: 147..505
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 192..501

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC06G124030.1CcUC06G124030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031119 tRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0003723 RNA binding
molecular_function GO:0106029 tRNA pseudouridine synthase activity
molecular_function GO:0009982 pseudouridine synthase activity