CcUC04G066350 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC04G066350
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
Descriptionvery-long-chain enoyl-CoA reductase-like
LocationCicolChr04: 23316203 .. 23317353 (-)
RNA-Seq ExpressionCcUC04G066350
SyntenyCcUC04G066350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAGATTACTGCGACGGAAATTTGGAGTACCTGACCATCACATTTAAGGATCTTGGTCCCCAGGTTTCTGATAGAATGCATCTTCTTGTTGGAGTATGTGGGGCCACTCCTTATCTATCCCATGTTTTACTACTTTCCCGTCTTTAAGTTCTTCGGCTATAAAGACGAGCATCATATCTACCCTGCTCAGACTTATGCCATGTACTATTGGTGTCTCCATTACTCTAAGCGGATCGTGGAAACCTTCTTTGTTCATAGGTTTAGTCATGTAACTTCACCCTTGTCTAATGTCTTCCGTAACTGTATGTACTATTGGAGTTTTGGAGCTCTGATAGGCTACTATGTTAACCACCCTCTTTACACCCCAGTGAATGACCTCCAAATGAAGATTGGCTTTGGATTTGGATTGTTATGTCAAATATCCAACTTATATTGCCATCTCATATTGAGGAATCTTAGAGATCCTAGTGGAAAGGGAGGCTACAAAATTCCACATGGGTACCTTTTCAATATTGTAACTTGTGCCAACTACACTACTGAGATCTATCAATGGCTTGGATTTAATATCGCTACACAAACTGTTGCTGGTTATATTTTTGTAATGGCGGCTACCTACATAATGACTAAATGGGCAATTGCGAAACACCGCCGCTTGGAAAAGGTGATTACTATTTGTCTCGTTTGTTCATGTAAAATGAACTCCTTCCATTGATATTTTGAAAGGCTCTTACTGTAACTTTCATGCATCTCGTAAGTTTTATGTTTAATTTAGTACACTTGGTCTTCAATTGTTGAAGTCTAAATCAAGCCGATTTTAATTTTGAAGTAGAAAATCTCTGCGTAGGATTATCTGTGCGGTCTAATTGATCTTAACCAACAAATTGTTCCATCATGAACAATTGCTTTGGTTTCTTATCATGATCTGTGTTTTTTAAATTAAAATTTGTCTATAAAAAGTGATCTTGCTGCATCTACAGTTTTTGTTCTTCCCTTATATTCTAATGCTGTCTTCCGCAGCTTTTCGATGGGAAGGAAGGGAGGCCAAAGTATCCAAGAAGATGGGTGATTCTACCTCCATTTTTCTGAAACAATAAAAAGCCTTCACTTGAGAAAATAATTGTAAGTCTGCAGTTGCAACTTGCAAGGCAAC

mRNA sequence

CAAAGATTACTGCGACGGAAATTTGGAGTACCTGACCATCACATTTAAGGATCTTGGTCCCCAGGTTTCTGATAGAATGCATCTTCTTGTTGGAGTATGTGGGGCCACTCCTTATCTATCCCATGTTTTACTACTTTCCCGTCTTTAAGTTCTTCGGCTATAAAGACGAGCATCATATCTACCCTGCTCAGACTTATGCCATGTACTATTGGTGTCTCCATTACTCTAAGCGGATCGTGGAAACCTTCTTTGTTCATAGGTTTAGTCATGTAACTTCACCCTTGTCTAATGTCTTCCGTAACTGTATGTACTATTGGAGTTTTGGAGCTCTGATAGGCTACTATGTTAACCACCCTCTTTACACCCCAGTGAATGACCTCCAAATGAAGATTGGCTTTGGATTTGGATTGTTATGTCAAATATCCAACTTATATTGCCATCTCATATTGAGGAATCTTAGAGATCCTAGTGGAAAGGGAGGCTACAAAATTCCACATGGGTACCTTTTCAATATTGTAACTTGTGCCAACTACACTACTGAGATCTATCAATGGCTTGGATTTAATATCGCTACACAAACTGTTGCTGGTTATATTTTTGTAATGGCGGCTACCTACATAATGACTAAATGGGCAATTGCGAAACACCGCCGCTTGGAAAAGCTTTTCGATGGGAAGGAAGGGAGGCCAAAGTATCCAAGAAGATGGGTGATTCTACCTCCATTTTTCTGAAACAATAAAAAGCCTTCACTTGAGAAAATAATTGTAAGTCTGCAGTTGCAACTTGCAAGGCAAC

Coding sequence (CDS)

ATGTTTTACTACTTTCCCGTCTTTAAGTTCTTCGGCTATAAAGACGAGCATCATATCTACCCTGCTCAGACTTATGCCATGTACTATTGGTGTCTCCATTACTCTAAGCGGATCGTGGAAACCTTCTTTGTTCATAGGTTTAGTCATGTAACTTCACCCTTGTCTAATGTCTTCCGTAACTGTATGTACTATTGGAGTTTTGGAGCTCTGATAGGCTACTATGTTAACCACCCTCTTTACACCCCAGTGAATGACCTCCAAATGAAGATTGGCTTTGGATTTGGATTGTTATGTCAAATATCCAACTTATATTGCCATCTCATATTGAGGAATCTTAGAGATCCTAGTGGAAAGGGAGGCTACAAAATTCCACATGGGTACCTTTTCAATATTGTAACTTGTGCCAACTACACTACTGAGATCTATCAATGGCTTGGATTTAATATCGCTACACAAACTGTTGCTGGTTATATTTTTGTAATGGCGGCTACCTACATAATGACTAAATGGGCAATTGCGAAACACCGCCGCTTGGAAAAGCTTTTCGATGGGAAGGAAGGGAGGCCAAAGTATCCAAGAAGATGGGTGATTCTACCTCCATTTTTCTGA

Protein sequence

MFYYFPVFKFFGYKDEHHIYPAQTYAMYYWCLHYSKRIVETFFVHRFSHVTSPLSNVFRNCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGGYKIPHGYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEKLFDGKEGRPKYPRRWVILPPFF
Homology
BLAST of CcUC04G066350 vs. NCBI nr
Match: XP_038877634.1 (very-long-chain enoyl-CoA reductase-like [Benincasa hispida])

HSP 1 Score: 414.1 bits (1063), Expect = 7.0e-112
Identity = 190/202 (94.06%), Postives = 197/202 (97.52%), Query Frame = 0

Query: 1   MFYYFPVFKFFGYKDEHHIYPAQTYAMYYWCLHYSKRIVETFFVHRFSHVTSPLSNVFRN 60
           MFYYFPV+KFFGY+DE HIYP QTYAMYYWCLHYSKRI+ETFFVHRFSH TSPLSNV RN
Sbjct: 109 MFYYFPVYKFFGYQDERHIYPVQTYAMYYWCLHYSKRIMETFFVHRFSHGTSPLSNVVRN 168

Query: 61  CMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGG 120
           CMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLIL+NLRDPSG+GG
Sbjct: 169 CMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILKNLRDPSGEGG 228

Query: 121 YKIPHGYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEK 180
           YKIP G+LFNIVTCANYTTEIYQWLGFNIATQTVAGYIFV+AATYIMTKWAIAKHRRLEK
Sbjct: 229 YKIPRGFLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVIAATYIMTKWAIAKHRRLEK 288

Query: 181 LFDGKEGRPKYPRRWVILPPFF 203
           LFDGKEGRPKYPRRWVILPPFF
Sbjct: 289 LFDGKEGRPKYPRRWVILPPFF 310

BLAST of CcUC04G066350 vs. NCBI nr
Match: XP_022143915.1 (very-long-chain enoyl-CoA reductase-like [Momordica charantia])

HSP 1 Score: 399.4 bits (1025), Expect = 1.8e-107
Identity = 181/200 (90.50%), Postives = 192/200 (96.00%), Query Frame = 0

Query: 3   YYFPVFKFFGYKDEHHIYPAQTYAMYYWCLHYSKRIVETFFVHRFSHVTSPLSNVFRNCM 62
           YYFPV+KFFGYK+E HIYP QTYAM+YWCLHYSKRI+ETFFVHRFSH TSPLSNV RNC 
Sbjct: 111 YYFPVYKFFGYKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCA 170

Query: 63  YYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGGYK 122
           YYWSFGALI YYVNHPLYTPV+DLQMKIGFGFGL+CQISNLYCHLIL+NLRDPSGKGGYK
Sbjct: 171 YYWSFGALIAYYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYK 230

Query: 123 IPHGYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEKLF 182
           IP G+LFNIVTCANYTTEIYQWLGFNIAT+TVAGYIFVMAA YIM+KWAIAKHRRLEKLF
Sbjct: 231 IPRGFLFNIVTCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLF 290

Query: 183 DGKEGRPKYPRRWVILPPFF 203
           DGKEGRP+YPRRWVILPPFF
Sbjct: 291 DGKEGRPRYPRRWVILPPFF 310

BLAST of CcUC04G066350 vs. NCBI nr
Match: XP_009403446.1 (PREDICTED: very-long-chain enoyl-CoA reductase [Musa acuminata subsp. malaccensis] >CAG1845487.1 unnamed protein product [Musa acuminata subsp. malaccensis])

HSP 1 Score: 378.3 bits (970), Expect = 4.3e-101
Identity = 164/201 (81.59%), Postives = 187/201 (93.03%), Query Frame = 0

Query: 1   MFYYFPVFKFFGYKDEHHIYPAQTYAMYYWCLHYSKRIVETFFVHRFSHVTSPLSNVFRN 60
           +FYYFPV+K+FGY+ E  +YP QTYAMYYWCLHYSKRI+ETFFVHRFSH TSPLSNVFRN
Sbjct: 109 IFYYFPVYKYFGYEGERVVYPVQTYAMYYWCLHYSKRIMETFFVHRFSHATSPLSNVFRN 168

Query: 61  CMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGG 120
           C YYW+FGA I YYVNHP+YTPVNDLQMKIGFGFGL+CQ+SN YCH++LRNLR+P+G GG
Sbjct: 169 CAYYWTFGAYIAYYVNHPMYTPVNDLQMKIGFGFGLICQVSNFYCHILLRNLRNPNGNGG 228

Query: 121 YKIPHGYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEK 180
           Y+IPHG+LFNIVTCANYTTEIYQWLGFNIATQT+AGY+F++ A  IMT WA+AKHRRL+K
Sbjct: 229 YQIPHGFLFNIVTCANYTTEIYQWLGFNIATQTIAGYVFLVVAALIMTNWALAKHRRLKK 288

Query: 181 LFDGKEGRPKYPRRWVILPPF 202
           LFDGKEGRP+YPRRWVILPPF
Sbjct: 289 LFDGKEGRPRYPRRWVILPPF 309

BLAST of CcUC04G066350 vs. NCBI nr
Match: XP_009591336.2 (very-long-chain enoyl-CoA reductase, partial [Nicotiana tomentosiformis])

HSP 1 Score: 373.6 bits (958), Expect = 1.1e-99
Identity = 166/201 (82.59%), Postives = 182/201 (90.55%), Query Frame = 0

Query: 1   MFYYFPVFKFFGYKDEHHIYPAQTYAMYYWCLHYSKRIVETFFVHRFSHVTSPLSNVFRN 60
           + YYFPV+KFFGYK+E  IYP QTYAMYYWC HY KRI+ETFFVHRFSH TSPLSNVFRN
Sbjct: 81  IIYYFPVYKFFGYKEERVIYPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRN 140

Query: 61  CMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGG 120
           C YYWSFGA I YYVNHPLYTPV+DLQMKIGFGFGL+CQ++N YCHL+LRNLR PSG GG
Sbjct: 141 CAYYWSFGAFIAYYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHLLLRNLRSPSGNGG 200

Query: 121 YKIPHGYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEK 180
           Y+IP+G+LFNIVTCANYTTEIYQWLGFNIATQTVAGY+F++ A  IMT WA+ KHRRL K
Sbjct: 201 YQIPYGFLFNIVTCANYTTEIYQWLGFNIATQTVAGYVFMVVAASIMTNWALGKHRRLRK 260

Query: 181 LFDGKEGRPKYPRRWVILPPF 202
           LFDGKEGRPKYPRRWVILPPF
Sbjct: 261 LFDGKEGRPKYPRRWVILPPF 281

BLAST of CcUC04G066350 vs. NCBI nr
Match: NP_001312551.1 (very-long-chain enoyl-CoA reductase-like [Nicotiana tabacum] >XP_016474926.1 PREDICTED: very-long-chain enoyl-CoA reductase-like [Nicotiana tabacum] >AFE88234.1 enoyl-CoA reductase [Nicotiana tabacum])

HSP 1 Score: 373.6 bits (958), Expect = 1.1e-99
Identity = 166/201 (82.59%), Postives = 182/201 (90.55%), Query Frame = 0

Query: 1   MFYYFPVFKFFGYKDEHHIYPAQTYAMYYWCLHYSKRIVETFFVHRFSHVTSPLSNVFRN 60
           + YYFPV+KFFGYK+E  IYP QTYAMYYWC HY KRI+ETFFVHRFSH TSPLSNVFRN
Sbjct: 109 IIYYFPVYKFFGYKEERVIYPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRN 168

Query: 61  CMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGG 120
           C YYWSFGA I YYVNHPLYTPV+DLQMKIGFGFGL+CQ++N YCHL+LRNLR PSG GG
Sbjct: 169 CAYYWSFGAFIAYYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHLLLRNLRSPSGNGG 228

Query: 121 YKIPHGYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEK 180
           Y+IP+G+LFNIVTCANYTTEIYQWLGFNIATQTVAGY+F++ A  IMT WA+ KHRRL K
Sbjct: 229 YQIPYGFLFNIVTCANYTTEIYQWLGFNIATQTVAGYVFMVVAASIMTNWALGKHRRLRK 288

Query: 181 LFDGKEGRPKYPRRWVILPPF 202
           LFDGKEGRPKYPRRWVILPPF
Sbjct: 289 LFDGKEGRPKYPRRWVILPPF 309

BLAST of CcUC04G066350 vs. ExPASy Swiss-Prot
Match: Q9M2U2 (Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana OX=3702 GN=ECR PE=1 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 1.3e-97
Identity = 155/201 (77.11%), Postives = 178/201 (88.56%), Query Frame = 0

Query: 1   MFYYFPVFKFFGYKDEHHIYPAQTYAMYYWCLHYSKRIVETFFVHRFSHVTSPLSNVFRN 60
           +FYYFPV+KF GY ++  I+P QTYAMYYWC HY KRI+ETFFVHRFSH TSP+ NVFRN
Sbjct: 109 VFYYFPVYKFLGYGEDCVIHPVQTYAMYYWCFHYFKRILETFFVHRFSHATSPIGNVFRN 168

Query: 61  CMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGG 120
           C YYWSFGA I YYVNHPLYTPV+DLQMKIGFGFGL+CQ++N YCH++L+NLRDPSG GG
Sbjct: 169 CAYYWSFGAYIAYYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHILLKNLRDPSGAGG 228

Query: 121 YKIPHGYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEK 180
           Y+IP G+LFNIVTCANYTTEIYQWLGFNIATQT+AGY+F+  A  IMT WA+ KH RL K
Sbjct: 229 YQIPRGFLFNIVTCANYTTEIYQWLGFNIATQTIAGYVFLAVAALIMTNWALGKHSRLRK 288

Query: 181 LFDGKEGRPKYPRRWVILPPF 202
           +FDGK+G+PKYPRRWVILPPF
Sbjct: 289 IFDGKDGKPKYPRRWVILPPF 309

BLAST of CcUC04G066350 vs. ExPASy Swiss-Prot
Match: Q55C17 (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn2 PE=3 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 1.4e-41
Identity = 90/202 (44.55%), Postives = 119/202 (58.91%), Query Frame = 0

Query: 3   YYFPVFKFFG---YKDEHHIYPAQTYAMYYWCLHYSKRIVETFFVHRFSHVTSPLSNVFR 62
           + +P+F F     Y  +     AQ  A+  + LHY KRI ET FVHRFSH T P+ N+F+
Sbjct: 103 FVYPIFYFLSNLIYGTDSPKSFAQKVALVCYSLHYIKRIYETIFVHRFSHGTMPIFNLFK 162

Query: 63  NCMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKG 122
           NC YYW   A++ Y+VNHPLYT     ++ +G G  ++ ++ N  CH+ LRNLR P+G  
Sbjct: 163 NCSYYWGCTAMVSYFVNHPLYTEAPIERVYLGLGLWIIGEVFNYICHIQLRNLR-PAGST 222

Query: 123 GYKIPHGYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLE 182
             KIP G LF  V+C NYT EI  W+GF+I TQT+  +IF +     M  WA+ KHRR  
Sbjct: 223 ERKIPRGLLFEFVSCPNYTVEILSWIGFSILTQTLTSWIFALMGAAQMWIWAVGKHRRYR 282

Query: 183 KLFDGKEGRPKYPRRWVILPPF 202
           K F       KYP+   IL PF
Sbjct: 283 KEFG-----DKYPKSRKILIPF 298

BLAST of CcUC04G066350 vs. ExPASy Swiss-Prot
Match: Q9N5Y2 (Probable very-long-chain enoyl-CoA reductase art-1 OS=Caenorhabditis elegans OX=6239 GN=art-1 PE=3 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.4e-33
Identity = 81/209 (38.76%), Postives = 111/209 (53.11%), Query Frame = 0

Query: 1   MFYYFPVFKFFGYKDEHHIYPAQTYAMYYWCLHYSKRIVETFFVHRFSHVTSPLSNVFRN 60
           +FY  P F +        ++PA   A + W  HY+KR+ ET F+HRF + T P  N+ +N
Sbjct: 105 LFYLRPTFIYGQAAVNATMHPAVQIAFFAWSFHYAKRLFETQFIHRFGNSTMPQFNLVKN 164

Query: 61  CMYYWSFGALIGYYVNHPLYTP--VNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGK 120
           C YYW F A + Y+VNHPL+TP    DLQ+  G    ++ +  NL  H++LRNLR P+G 
Sbjct: 165 CSYYWGFAAFVAYFVNHPLFTPPAFGDLQVYFGLAGFVISEFGNLSIHILLRNLR-PAGT 224

Query: 121 GGYKIPH------GYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAI 180
              +IP         LFN V+C NYT E+  W+ F+I  Q++   IF  A    M  WA 
Sbjct: 225 RERRIPKPDGNPLSLLFNYVSCPNYTYEVASWIFFSIMVQSLPAIIFTTAGFAQMAIWAQ 284

Query: 181 AKHRRLEKLFDGKEGRPKYPRRWVILPPF 202
            KHR   K F      P YP+    + PF
Sbjct: 285 GKHRNYLKEF------PDYPKNRKAIVPF 306

BLAST of CcUC04G066350 vs. ExPASy Swiss-Prot
Match: Q9NZ01 (Very-long-chain enoyl-CoA reductase OS=Homo sapiens OX=9606 GN=TECR PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 6.8e-33
Identity = 84/211 (39.81%), Postives = 118/211 (55.92%), Query Frame = 0

Query: 1   MFYYFPVFKFFGYKDEHHIYPAQTYAMYYWCL----HYSKRIVETFFVHRFSHVTSPLSN 60
           + +YF V   +G+K  +    ++   ++  C+    HY KR++ET FVHRFSH T PL N
Sbjct: 103 LLFYFRVPFIYGHK--YDFTSSRHTVVHLACICHSFHYIKRLLETLFVHRFSHGTMPLRN 162

Query: 61  VFRNCMYYWSFGALIGYYVNHPLYTP--VNDLQMKIGFGFGLLCQISNLYCHLILRNLRD 120
           +F+NC YYW F A + YY+NHPLYTP      Q+K+     ++CQ+ N   H+ LR+LR 
Sbjct: 163 IFKNCTYYWGFAAWMAYYINHPLYTPPTYGAQQVKLALAIFVICQLGNFSIHMALRDLR- 222

Query: 121 PSGKGGYKIPH------GYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMT 180
           P+G    KIP+       +LF +V+C NYT E+  W+GF I TQ +   +F +     MT
Sbjct: 223 PAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMT 282

Query: 181 KWAIAKHRRLEKLFDGKEGRPKYPRRWVILP 200
            WA  KHR   K F     R   P R  I+P
Sbjct: 283 IWAKGKHRSYLKEF-----RDYPPLRMPIIP 305

BLAST of CcUC04G066350 vs. ExPASy Swiss-Prot
Match: Q64232 (Very-long-chain enoyl-CoA reductase OS=Rattus norvegicus OX=10116 GN=Tecr PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.2e-32
Identity = 85/211 (40.28%), Postives = 117/211 (55.45%), Query Frame = 0

Query: 1   MFYYFPVFKFFGYKDEHHIYPAQTYAMYYWCL----HYSKRIVETFFVHRFSHVTSPLSN 60
           + +YF V   +G K  +    ++   ++  C+    HY KR++ET FVHRFSH T PL N
Sbjct: 103 LLFYFRVPFIYGRK--YDFTSSRHTVVHLACMCHSFHYIKRLLETLFVHRFSHGTMPLRN 162

Query: 61  VFRNCMYYWSFGALIGYYVNHPLYTP--VNDLQMKIGFGFGLLCQISNLYCHLILRNLRD 120
           +F+NC YYW F A + YY+NHPLYTP      Q+K+     ++CQ+ N   H+ LR+LR 
Sbjct: 163 IFKNCTYYWGFAAWMAYYINHPLYTPPTYGVQQVKLALAIFVICQLGNFSIHMALRDLR- 222

Query: 121 PSGKGGYKIPH------GYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMT 180
           P+G    KIP+       +LF +V+C NYT E+  W+GF I TQ V   +F +     MT
Sbjct: 223 PAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCVPVALFSLVGFTQMT 282

Query: 181 KWAIAKHRRLEKLFDGKEGRPKYPRRWVILP 200
            WA  KHR   K F     R   P R  I+P
Sbjct: 283 IWAKGKHRSYLKEF-----RDYPPLRMPIIP 305

BLAST of CcUC04G066350 vs. ExPASy TrEMBL
Match: A0A6J1CQ63 (very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC111013716 PE=3 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 8.7e-108
Identity = 181/200 (90.50%), Postives = 192/200 (96.00%), Query Frame = 0

Query: 3   YYFPVFKFFGYKDEHHIYPAQTYAMYYWCLHYSKRIVETFFVHRFSHVTSPLSNVFRNCM 62
           YYFPV+KFFGYK+E HIYP QTYAM+YWCLHYSKRI+ETFFVHRFSH TSPLSNV RNC 
Sbjct: 111 YYFPVYKFFGYKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCA 170

Query: 63  YYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGGYK 122
           YYWSFGALI YYVNHPLYTPV+DLQMKIGFGFGL+CQISNLYCHLIL+NLRDPSGKGGYK
Sbjct: 171 YYWSFGALIAYYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYK 230

Query: 123 IPHGYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEKLF 182
           IP G+LFNIVTCANYTTEIYQWLGFNIAT+TVAGYIFVMAA YIM+KWAIAKHRRLEKLF
Sbjct: 231 IPRGFLFNIVTCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLF 290

Query: 183 DGKEGRPKYPRRWVILPPFF 203
           DGKEGRP+YPRRWVILPPFF
Sbjct: 291 DGKEGRPRYPRRWVILPPFF 310

BLAST of CcUC04G066350 vs. ExPASy TrEMBL
Match: M0T4A4 (S5A_REDUCTASE domain-containing protein OS=Musa acuminata subsp. malaccensis OX=214687 GN=103987000 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 2.1e-101
Identity = 164/201 (81.59%), Postives = 187/201 (93.03%), Query Frame = 0

Query: 1   MFYYFPVFKFFGYKDEHHIYPAQTYAMYYWCLHYSKRIVETFFVHRFSHVTSPLSNVFRN 60
           +FYYFPV+K+FGY+ E  +YP QTYAMYYWCLHYSKRI+ETFFVHRFSH TSPLSNVFRN
Sbjct: 109 IFYYFPVYKYFGYEGERVVYPVQTYAMYYWCLHYSKRIMETFFVHRFSHATSPLSNVFRN 168

Query: 61  CMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGG 120
           C YYW+FGA I YYVNHP+YTPVNDLQMKIGFGFGL+CQ+SN YCH++LRNLR+P+G GG
Sbjct: 169 CAYYWTFGAYIAYYVNHPMYTPVNDLQMKIGFGFGLICQVSNFYCHILLRNLRNPNGNGG 228

Query: 121 YKIPHGYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEK 180
           Y+IPHG+LFNIVTCANYTTEIYQWLGFNIATQT+AGY+F++ A  IMT WA+AKHRRL+K
Sbjct: 229 YQIPHGFLFNIVTCANYTTEIYQWLGFNIATQTIAGYVFLVVAALIMTNWALAKHRRLKK 288

Query: 181 LFDGKEGRPKYPRRWVILPPF 202
           LFDGKEGRP+YPRRWVILPPF
Sbjct: 289 LFDGKEGRPRYPRRWVILPPF 309

BLAST of CcUC04G066350 vs. ExPASy TrEMBL
Match: H9CCI1 (Enoyl-CoA reductase OS=Nicotiana tabacum OX=4097 GN=ER PE=2 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 5.1e-100
Identity = 166/201 (82.59%), Postives = 182/201 (90.55%), Query Frame = 0

Query: 1   MFYYFPVFKFFGYKDEHHIYPAQTYAMYYWCLHYSKRIVETFFVHRFSHVTSPLSNVFRN 60
           + YYFPV+KFFGYK+E  IYP QTYAMYYWC HY KRI+ETFFVHRFSH TSPLSNVFRN
Sbjct: 109 IIYYFPVYKFFGYKEERVIYPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRN 168

Query: 61  CMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGG 120
           C YYWSFGA I YYVNHPLYTPV+DLQMKIGFGFGL+CQ++N YCHL+LRNLR PSG GG
Sbjct: 169 CAYYWSFGAFIAYYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHLLLRNLRSPSGNGG 228

Query: 121 YKIPHGYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEK 180
           Y+IP+G+LFNIVTCANYTTEIYQWLGFNIATQTVAGY+F++ A  IMT WA+ KHRRL K
Sbjct: 229 YQIPYGFLFNIVTCANYTTEIYQWLGFNIATQTVAGYVFMVVAASIMTNWALGKHRRLRK 288

Query: 181 LFDGKEGRPKYPRRWVILPPF 202
           LFDGKEGRPKYPRRWVILPPF
Sbjct: 289 LFDGKEGRPKYPRRWVILPPF 309

BLAST of CcUC04G066350 vs. ExPASy TrEMBL
Match: V7B6X6 (S5A_REDUCTASE domain-containing protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_008G208700g PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 6.7e-100
Identity = 164/201 (81.59%), Postives = 185/201 (92.04%), Query Frame = 0

Query: 1   MFYYFPVFKFFGYKDEHHIYPAQTYAMYYWCLHYSKRIVETFFVHRFSHVTSPLSNVFRN 60
           +FYYFPV+K+FGYK E  I+P QTYA+YYWC HY+KRI+ETFFVHRFSH TSPLSNVFRN
Sbjct: 108 VFYYFPVYKYFGYKGERVIHPVQTYALYYWCFHYAKRILETFFVHRFSHATSPLSNVFRN 167

Query: 61  CMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGG 120
           C YYW+FG+ I YYVNHPLYTPVNDLQMKIGFGFG+LCQISN YCH+IL+NLR P G+GG
Sbjct: 168 CAYYWTFGSYIAYYVNHPLYTPVNDLQMKIGFGFGILCQISNFYCHIILKNLRSPGGEGG 227

Query: 121 YKIPHGYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEK 180
           Y+IP G+LFNIVTCANYTTEIYQWLGFNIATQTVAGYIF+  AT+IMT WA+AKHRRL+K
Sbjct: 228 YQIPKGFLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFLAVATFIMTNWALAKHRRLKK 287

Query: 181 LFDGKEGRPKYPRRWVILPPF 202
           LFDGKEGRP+YPRRW+ILPPF
Sbjct: 288 LFDGKEGRPRYPRRWIILPPF 308

BLAST of CcUC04G066350 vs. ExPASy TrEMBL
Match: Q3IAA0 (TSC13 protein OS=Nicotiana benthamiana OX=4100 GN=TSC13 PE=2 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.9e-99
Identity = 165/201 (82.09%), Postives = 182/201 (90.55%), Query Frame = 0

Query: 1   MFYYFPVFKFFGYKDEHHIYPAQTYAMYYWCLHYSKRIVETFFVHRFSHVTSPLSNVFRN 60
           + YYFPV+KFFGYK+E  I+P QTYAMYYWC HY KRI+ETFFVHRFSH TSPLSNVFRN
Sbjct: 109 IIYYFPVYKFFGYKEERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRN 168

Query: 61  CMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGG 120
           C YYWSFGA I YYVNHPLYTPV+DLQMKIGFGFGL+CQ++N YCHL+LRNLR PSG GG
Sbjct: 169 CAYYWSFGAFIAYYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHLLLRNLRSPSGNGG 228

Query: 121 YKIPHGYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEK 180
           Y+IP+G+LFNIVTCANYTTEIYQWLGFNIATQTVAGY+F++ A  IMT WA+ KHRRL K
Sbjct: 229 YQIPYGFLFNIVTCANYTTEIYQWLGFNIATQTVAGYVFMVVAASIMTNWALGKHRRLRK 288

Query: 181 LFDGKEGRPKYPRRWVILPPF 202
           LFDGKEGRPKYPRRWVILPPF
Sbjct: 289 LFDGKEGRPKYPRRWVILPPF 309

BLAST of CcUC04G066350 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 357.1 bits (915), Expect = 9.5e-99
Identity = 155/201 (77.11%), Postives = 178/201 (88.56%), Query Frame = 0

Query: 1   MFYYFPVFKFFGYKDEHHIYPAQTYAMYYWCLHYSKRIVETFFVHRFSHVTSPLSNVFRN 60
           +FYYFPV+KF GY ++  I+P QTYAMYYWC HY KRI+ETFFVHRFSH TSP+ NVFRN
Sbjct: 109 VFYYFPVYKFLGYGEDCVIHPVQTYAMYYWCFHYFKRILETFFVHRFSHATSPIGNVFRN 168

Query: 61  CMYYWSFGALIGYYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILRNLRDPSGKGG 120
           C YYWSFGA I YYVNHPLYTPV+DLQMKIGFGFGL+CQ++N YCH++L+NLRDPSG GG
Sbjct: 169 CAYYWSFGAYIAYYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHILLKNLRDPSGAGG 228

Query: 121 YKIPHGYLFNIVTCANYTTEIYQWLGFNIATQTVAGYIFVMAATYIMTKWAIAKHRRLEK 180
           Y+IP G+LFNIVTCANYTTEIYQWLGFNIATQT+AGY+F+  A  IMT WA+ KH RL K
Sbjct: 229 YQIPRGFLFNIVTCANYTTEIYQWLGFNIATQTIAGYVFLAVAALIMTNWALGKHSRLRK 288

Query: 181 LFDGKEGRPKYPRRWVILPPF 202
           +FDGK+G+PKYPRRWVILPPF
Sbjct: 289 IFDGKDGKPKYPRRWVILPPF 309

BLAST of CcUC04G066350 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 73.2 bits (178), Expect = 2.7e-13
Identity = 47/151 (31.13%), Postives = 74/151 (49.01%), Query Frame = 0

Query: 32  LHYSKRIVETFFVHRFSHVTSPLSNVFRNCMYYWSFGALIGYYVNHPLYTPVNDLQMKI- 91
           LH+ KR+ E  F+H++S   + + +       Y+S  AL+ Y  N  L        MK+ 
Sbjct: 103 LHFFKRVFEVLFIHKYSGGMA-IDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLA 162

Query: 92  GFGFGLLCQISNLYCHLILRNLRDPSGKGGYKIPHGYLFNIVTCANYTTEIYQWLGFNIA 151
           G    ++  + NLY H++L  LR   GK  YKIP G LF+I+ C +Y  EI  +  F + 
Sbjct: 163 GVVMFVVGIVGNLYHHVLLAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLI 222

Query: 152 TQTVAGYIFVMAA-------TYIMTKWAIAK 175
           +QT+  + F M         +Y    W ++K
Sbjct: 223 SQTIYSFSFAMGTMLYLIGRSYATRTWYLSK 252

BLAST of CcUC04G066350 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 46.2 bits (108), Expect = 3.6e-05
Identity = 33/114 (28.95%), Postives = 54/114 (47.37%), Query Frame = 0

Query: 92  FGFGLLCQISNLYCHL----ILRNLRDPSGKGGYKIPHGYLFNIVTCANYTTEIYQWLGF 151
           F  G++  I+ +Y ++     L  L+    +GGY IP G  F +V+C NY  E  +WLG+
Sbjct: 153 FVIGMVVFITGMYINITSDRTLVRLK-KENRGGYVIPRGGWFELVSCPNYFGEAIEWLGW 212

Query: 152 NIATQTVAGYIFVMAATYIMTKWAIAKHRRLEKLFDGKEGRPKYPRRWVILPPF 202
            + T + AG  F +     +   A A H+     F     + +YP+    + PF
Sbjct: 213 AVMTWSWAGIGFFLYTCSNLFPRARASHKWYIAKF-----KEEYPKTRKAVIPF 260

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038877634.17.0e-11294.06very-long-chain enoyl-CoA reductase-like [Benincasa hispida][more]
XP_022143915.11.8e-10790.50very-long-chain enoyl-CoA reductase-like [Momordica charantia][more]
XP_009403446.14.3e-10181.59PREDICTED: very-long-chain enoyl-CoA reductase [Musa acuminata subsp. malaccensi... [more]
XP_009591336.21.1e-9982.59very-long-chain enoyl-CoA reductase, partial [Nicotiana tomentosiformis][more]
NP_001312551.11.1e-9982.59very-long-chain enoyl-CoA reductase-like [Nicotiana tabacum] >XP_016474926.1 PRE... [more]
Match NameE-valueIdentityDescription
Q9M2U21.3e-9777.11Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana OX=3702 GN=ECR PE=1 ... [more]
Q55C171.4e-4144.55Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn... [more]
Q9N5Y21.4e-3338.76Probable very-long-chain enoyl-CoA reductase art-1 OS=Caenorhabditis elegans OX=... [more]
Q9NZ016.8e-3339.81Very-long-chain enoyl-CoA reductase OS=Homo sapiens OX=9606 GN=TECR PE=1 SV=1[more]
Q642321.2e-3240.28Very-long-chain enoyl-CoA reductase OS=Rattus norvegicus OX=10116 GN=Tecr PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1CQ638.7e-10890.50very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC11... [more]
M0T4A42.1e-10181.59S5A_REDUCTASE domain-containing protein OS=Musa acuminata subsp. malaccensis OX=... [more]
H9CCI15.1e-10082.59Enoyl-CoA reductase OS=Nicotiana tabacum OX=4097 GN=ER PE=2 SV=1[more]
V7B6X66.7e-10081.59S5A_REDUCTASE domain-containing protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_0... [more]
Q3IAA01.9e-9982.09TSC13 protein OS=Nicotiana benthamiana OX=4100 GN=TSC13 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G55360.19.5e-9977.113-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT5G16010.12.7e-1331.133-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT2G38050.13.6e-0528.953-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPFAMPF02544Steroid_dhcoord: 51..201
e-value: 4.0E-28
score: 98.3
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPROSITEPS50244S5A_REDUCTASEcoord: 91..172
score: 21.021988
IPR0393573-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductasePANTHERPTHR105563-OXO-5-ALPHA-STEROID 4-DEHYDROGENASEcoord: 1..202
NoneNo IPR availablePANTHERPTHR10556:SF28SC2coord: 1..202

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC04G066350.1CcUC04G066350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042761 very long-chain fatty acid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors