Carg27810 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg27810 vs. NCBI nr
Match: KAG7023926.1 (hypothetical protein SDJN02_14954 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1171.8 bits (3030), Expect = 0.0e+00
Identity = 603/603 (100.00%), Postives = 603/603 (100.00%), Query Frame = 0

Query: 1   MEDKCAIKTDATELIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDRIALSMIQDAE 60
           MEDKCAIKTDATELIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDRIALSMIQDAE
Sbjct: 1   MEDKCAIKTDATELIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDRIALSMIQDAE 60

Query: 61  DKGLISPGKGYRLILIMPAFFSMERRILLLSFGAELHITNPEMSVDAVIKMAEDLEKSTP 120
           DKGLISPGKGYRLILIMPAFFSMERRILLLSFGAELHITNPEMSVDAVIKMAEDLEKSTP
Sbjct: 61  DKGLISPGKGYRLILIMPAFFSMERRILLLSFGAELHITNPEMSVDAVIKMAEDLEKSTP 120

Query: 121 NGYFLRQFDNPANAKVYGIEPVESAVLSGGQPGHHLIQGIGAGFIPSILDIKLIDEVIQI 180
           NGYFLRQFDNPANAKVYGIEPVESAVLSGGQPGHHLIQGIGAGFIPSILDIKLIDEVIQI
Sbjct: 121 NGYFLRQFDNPANAKVYGIEPVESAVLSGGQPGHHLIQGIGAGFIPSILDIKLIDEVIQI 180

Query: 181 STEEAIETAKLLASKEGLLVGFSSGAAAAAAIKIAKRPENEGKLIVVIFPKLRRAKFVAS 240
           STEEAIETAKLLASKEGLLVGFSSGAAAAAAIKIAKRPENEGKLIVVIFPKLRRAKFVAS
Sbjct: 181 STEEAIETAKLLASKEGLLVGFSSGAAAAAAIKIAKRPENEGKLIVVIFPKLRRAKFVAS 240

Query: 241 KSYFYVILCPFFCSVFLPFLFPRSLVVYAETNLTELMEDKCAIKTDATEVIFTYWAACLF 300
           KSYFYVILCPFFCSVFLPFLFPRSLVVYAETNLTELMEDKCAIKTDATEVIFTYWAACLF
Sbjct: 241 KSYFYVILCPFFCSVFLPFLFPRSLVVYAETNLTELMEDKCAIKTDATEVIFTYWAACLF 300

Query: 301 SFPFLDGWFLGLQLIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDRVLIEATAGNT 360
           SFPFLDGWFLGLQLIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDRVLIEATAGNT
Sbjct: 301 SFPFLDGWFLGLQLIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDRVLIEATAGNT 360

Query: 361 GIGLAWIAAIKGYRLILVMPAFFSMERRILLLAFGAELHVTNPEMSGDAILKIAEDLEKS 420
           GIGLAWIAAIKGYRLILVMPAFFSMERRILLLAFGAELHVTNPEMSGDAILKIAEDLEKS
Sbjct: 361 GIGLAWIAAIKGYRLILVMPAFFSMERRILLLAFGAELHVTNPEMSGDAILKIAEDLEKS 420

Query: 421 TPNGYFLRQFDNPANTMIHYEATGVEIWKDSNGKVDALVAGIGTGGTLTGAGKFLKEQNP 480
           TPNGYFLRQFDNPANTMIHYEATGVEIWKDSNGKVDALVAGIGTGGTLTGAGKFLKEQNP
Sbjct: 421 TPNGYFLRQFDNPANTMIHYEATGVEIWKDSNGKVDALVAGIGTGGTLTGAGKFLKEQNP 480

Query: 481 KIKVYGIEPVESAVLSGGQPGRHLIQGIGAGIIPSILDVELLDEVIQISSEEAIETAKLI 540
           KIKVYGIEPVESAVLSGGQPGRHLIQGIGAGIIPSILDVELLDEVIQISSEEAIETAKLI
Sbjct: 481 KIKVYGIEPVESAVLSGGQPGRHLIQGIGAGIIPSILDVELLDEVIQISSEEAIETAKLI 540

Query: 541 ALKEGLLVGISSGAAAAAAIKVAKRPENEGKLIVVIFPSFGERYLSTSLFDAARKEAENI 600
           ALKEGLLVGISSGAAAAAAIKVAKRPENEGKLIVVIFPSFGERYLSTSLFDAARKEAENI
Sbjct: 541 ALKEGLLVGISSGAAAAAAIKVAKRPENEGKLIVVIFPSFGERYLSTSLFDAARKEAENI 600

Query: 601 KYL 604
           KYL
Sbjct: 601 KYL 603

BLAST of Carg27810 vs. NCBI nr
Match: GAY54165.1 (hypothetical protein CUMW_154590 [Citrus unshiu])

HSP 1 Score: 703.0 bits (1813), Expect = 2.3e-198
Identity = 389/697 (55.81%), Postives = 470/697 (67.43%), Query Frame = 0

Query: 1    MEDKCAIKTDATELIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDRIALSMIQDAE 60
            MEDKC IK D TELIG+TPMVYLN +VDGC A+IAAKLE M+PCSSVKDRIA SMI+DAE
Sbjct: 592  MEDKCEIKKDVTELIGHTPMVYLNNVVDGCVARIAAKLEMMQPCSSVKDRIAYSMIKDAE 651

Query: 61   DKGLISPGK----------------------GYRLILIMPAFFSMERRILLLSFGAELHI 120
            DKGLI+PGK                      GY+LI+IMP+ +S+ERRI+L + GAE+++
Sbjct: 652  DKGLITPGKTVLIELTSGNTGIGLAFIAASRGYKLIIIMPSTYSIERRIILRALGAEVYL 711

Query: 121  TNPEMSVDAVIKMAEDLEKSTPNGYFLRQFDNPA-------------------------- 180
             +P +  +  +K  E++   TPNGY L QF+NPA                          
Sbjct: 712  ADPAVGFEGFVKKGEEILNRTPNGYILGQFENPANPEIHYETTGPEIWNDSGGKVDAFIA 771

Query: 181  --------------------NAKVYGIEPVESAVLSGGQPGHHLIQGIGAGFIPSILDIK 240
                                N KVYGIEP ESAVL+GGQPG HLIQGIGAG IP +LD+ 
Sbjct: 772  GIGTGGTVTGAGRFLKEKNPNIKVYGIEPSESAVLNGGQPGKHLIQGIGAGVIPPVLDVA 831

Query: 241  LIDEVIQISTEEAIETAKLLASKEGLLVGFSSGAAAAAAIKIAKRPENEGKLIVVIFPKL 300
            ++DEVI +S+EEAIET+KLLA KEGLLVG SSGAAAAAAI++AKRPEN GKLIVVIFP  
Sbjct: 832  MLDEVITVSSEEAIETSKLLALKEGLLVGISSGAAAAAAIRVAKRPENAGKLIVVIFPS- 891

Query: 301  RRAKFVASKSYFYVILCPFFCSVFLPFLFPRSLVVYAETNLTELMEDKCAIKTDATEVIF 360
                  A + Y                                 MEDKC IK D TEVIF
Sbjct: 892  ------AGERYLSTAFS---------------------------MEDKCEIKKDVTEVIF 951

Query: 361  TYWAACLFSFPFLDGW---------FLGLQLIGNTPMVYLNKIVDGCFAKIAAKLESMEP 420
             +    L + P + G+         F  +QLIG+TPMVYLN +VDGC A+IAAKLE M+P
Sbjct: 952  -FAQLMLLNLPSVFGYCDLAYRLAVFWLVQLIGHTPMVYLNNVVDGCVARIAAKLEMMQP 1011

Query: 421  CSSVKDR--------------------VLIEATAGNTGIGLAWIAAIKGYRLILVMPAFF 480
            CSSVKDR                    VLIE T+GNTGIGLA+IAA +GY+LI++MP+ +
Sbjct: 1012 CSSVKDRIAYSMVKDAEDKGLITPGKTVLIEITSGNTGIGLAFIAASRGYKLIIIMPSTY 1071

Query: 481  SMERRILLLAFGAELHVTNPEMSGDAILKIAEDLEKSTPNGYFLRQFDNPANTMIHYEAT 540
            S+ERRI+L A GAE+++ +P +  + ++K  E++   TPNGY L QF+NPAN  IHYE T
Sbjct: 1072 SIERRIILRALGAEVYLADPAVGFEGLVKKGEEILNRTPNGYILGQFENPANPEIHYETT 1131

Query: 541  GVEIWKDSNGKVDALVAGIGTGGTLTGAGKFLKEQNPKIKVYGIEPVESAVLSGGQPGRH 600
            G EIW DS GKV A +AGIGTGGT+TGAG+FLKE+NP IKVYGIEP ESA+L+GGQPG+H
Sbjct: 1132 GPEIWNDSGGKVAAFIAGIGTGGTVTGAGRFLKEKNPNIKVYGIEPSESAILNGGQPGKH 1191

BLAST of Carg27810 vs. NCBI nr
Match: GAY54164.1 (hypothetical protein CUMW_154590 [Citrus unshiu])

HSP 1 Score: 703.0 bits (1813), Expect = 2.3e-198
Identity = 389/697 (55.81%), Postives = 470/697 (67.43%), Query Frame = 0

Query: 1    MEDKCAIKTDATELIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDRIALSMIQDAE 60
            MEDKC IK D TELIG+TPMVYLN +VDGC A+IAAKLE M+PCSSVKDRIA SMI+DAE
Sbjct: 636  MEDKCEIKKDVTELIGHTPMVYLNNVVDGCVARIAAKLEMMQPCSSVKDRIAYSMIKDAE 695

Query: 61   DKGLISPGK----------------------GYRLILIMPAFFSMERRILLLSFGAELHI 120
            DKGLI+PGK                      GY+LI+IMP+ +S+ERRI+L + GAE+++
Sbjct: 696  DKGLITPGKTVLIELTSGNTGIGLAFIAASRGYKLIIIMPSTYSIERRIILRALGAEVYL 755

Query: 121  TNPEMSVDAVIKMAEDLEKSTPNGYFLRQFDNPA-------------------------- 180
             +P +  +  +K  E++   TPNGY L QF+NPA                          
Sbjct: 756  ADPAVGFEGFVKKGEEILNRTPNGYILGQFENPANPEIHYETTGPEIWNDSGGKVDAFIA 815

Query: 181  --------------------NAKVYGIEPVESAVLSGGQPGHHLIQGIGAGFIPSILDIK 240
                                N KVYGIEP ESAVL+GGQPG HLIQGIGAG IP +LD+ 
Sbjct: 816  GIGTGGTVTGAGRFLKEKNPNIKVYGIEPSESAVLNGGQPGKHLIQGIGAGVIPPVLDVA 875

Query: 241  LIDEVIQISTEEAIETAKLLASKEGLLVGFSSGAAAAAAIKIAKRPENEGKLIVVIFPKL 300
            ++DEVI +S+EEAIET+KLLA KEGLLVG SSGAAAAAAI++AKRPEN GKLIVVIFP  
Sbjct: 876  MLDEVITVSSEEAIETSKLLALKEGLLVGISSGAAAAAAIRVAKRPENAGKLIVVIFPS- 935

Query: 301  RRAKFVASKSYFYVILCPFFCSVFLPFLFPRSLVVYAETNLTELMEDKCAIKTDATEVIF 360
                  A + Y                                 MEDKC IK D TEVIF
Sbjct: 936  ------AGERYLSTAFS---------------------------MEDKCEIKKDVTEVIF 995

Query: 361  TYWAACLFSFPFLDGW---------FLGLQLIGNTPMVYLNKIVDGCFAKIAAKLESMEP 420
             +    L + P + G+         F  +QLIG+TPMVYLN +VDGC A+IAAKLE M+P
Sbjct: 996  -FAQLMLLNLPSVFGYCDLAYRLAVFWLVQLIGHTPMVYLNNVVDGCVARIAAKLEMMQP 1055

Query: 421  CSSVKDR--------------------VLIEATAGNTGIGLAWIAAIKGYRLILVMPAFF 480
            CSSVKDR                    VLIE T+GNTGIGLA+IAA +GY+LI++MP+ +
Sbjct: 1056 CSSVKDRIAYSMVKDAEDKGLITPGKTVLIEITSGNTGIGLAFIAASRGYKLIIIMPSTY 1115

Query: 481  SMERRILLLAFGAELHVTNPEMSGDAILKIAEDLEKSTPNGYFLRQFDNPANTMIHYEAT 540
            S+ERRI+L A GAE+++ +P +  + ++K  E++   TPNGY L QF+NPAN  IHYE T
Sbjct: 1116 SIERRIILRALGAEVYLADPAVGFEGLVKKGEEILNRTPNGYILGQFENPANPEIHYETT 1175

Query: 541  GVEIWKDSNGKVDALVAGIGTGGTLTGAGKFLKEQNPKIKVYGIEPVESAVLSGGQPGRH 600
            G EIW DS GKV A +AGIGTGGT+TGAG+FLKE+NP IKVYGIEP ESA+L+GGQPG+H
Sbjct: 1176 GPEIWNDSGGKVAAFIAGIGTGGTVTGAGRFLKEKNPNIKVYGIEPSESAILNGGQPGKH 1235

BLAST of Carg27810 vs. NCBI nr
Match: GAY54166.1 (hypothetical protein CUMW_154590 [Citrus unshiu])

HSP 1 Score: 703.0 bits (1813), Expect = 2.3e-198
Identity = 389/697 (55.81%), Postives = 470/697 (67.43%), Query Frame = 0

Query: 1   MEDKCAIKTDATELIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDRIALSMIQDAE 60
           MEDKC IK D TELIG+TPMVYLN +VDGC A+IAAKLE M+PCSSVKDRIA SMI+DAE
Sbjct: 302 MEDKCEIKKDVTELIGHTPMVYLNNVVDGCVARIAAKLEMMQPCSSVKDRIAYSMIKDAE 361

Query: 61  DKGLISPGK----------------------GYRLILIMPAFFSMERRILLLSFGAELHI 120
           DKGLI+PGK                      GY+LI+IMP+ +S+ERRI+L + GAE+++
Sbjct: 362 DKGLITPGKTVLIELTSGNTGIGLAFIAASRGYKLIIIMPSTYSIERRIILRALGAEVYL 421

Query: 121 TNPEMSVDAVIKMAEDLEKSTPNGYFLRQFDNPA-------------------------- 180
            +P +  +  +K  E++   TPNGY L QF+NPA                          
Sbjct: 422 ADPAVGFEGFVKKGEEILNRTPNGYILGQFENPANPEIHYETTGPEIWNDSGGKVDAFIA 481

Query: 181 --------------------NAKVYGIEPVESAVLSGGQPGHHLIQGIGAGFIPSILDIK 240
                               N KVYGIEP ESAVL+GGQPG HLIQGIGAG IP +LD+ 
Sbjct: 482 GIGTGGTVTGAGRFLKEKNPNIKVYGIEPSESAVLNGGQPGKHLIQGIGAGVIPPVLDVA 541

Query: 241 LIDEVIQISTEEAIETAKLLASKEGLLVGFSSGAAAAAAIKIAKRPENEGKLIVVIFPKL 300
           ++DEVI +S+EEAIET+KLLA KEGLLVG SSGAAAAAAI++AKRPEN GKLIVVIFP  
Sbjct: 542 MLDEVITVSSEEAIETSKLLALKEGLLVGISSGAAAAAAIRVAKRPENAGKLIVVIFPS- 601

Query: 301 RRAKFVASKSYFYVILCPFFCSVFLPFLFPRSLVVYAETNLTELMEDKCAIKTDATEVIF 360
                 A + Y                                 MEDKC IK D TEVIF
Sbjct: 602 ------AGERYLSTAFS---------------------------MEDKCEIKKDVTEVIF 661

Query: 361 TYWAACLFSFPFLDGW---------FLGLQLIGNTPMVYLNKIVDGCFAKIAAKLESMEP 420
            +    L + P + G+         F  +QLIG+TPMVYLN +VDGC A+IAAKLE M+P
Sbjct: 662 -FAQLMLLNLPSVFGYCDLAYRLAVFWLVQLIGHTPMVYLNNVVDGCVARIAAKLEMMQP 721

Query: 421 CSSVKDR--------------------VLIEATAGNTGIGLAWIAAIKGYRLILVMPAFF 480
           CSSVKDR                    VLIE T+GNTGIGLA+IAA +GY+LI++MP+ +
Sbjct: 722 CSSVKDRIAYSMVKDAEDKGLITPGKTVLIEITSGNTGIGLAFIAASRGYKLIIIMPSTY 781

Query: 481 SMERRILLLAFGAELHVTNPEMSGDAILKIAEDLEKSTPNGYFLRQFDNPANTMIHYEAT 540
           S+ERRI+L A GAE+++ +P +  + ++K  E++   TPNGY L QF+NPAN  IHYE T
Sbjct: 782 SIERRIILRALGAEVYLADPAVGFEGLVKKGEEILNRTPNGYILGQFENPANPEIHYETT 841

Query: 541 GVEIWKDSNGKVDALVAGIGTGGTLTGAGKFLKEQNPKIKVYGIEPVESAVLSGGQPGRH 600
           G EIW DS GKV A +AGIGTGGT+TGAG+FLKE+NP IKVYGIEP ESA+L+GGQPG+H
Sbjct: 842 GPEIWNDSGGKVAAFIAGIGTGGTVTGAGRFLKEKNPNIKVYGIEPSESAILNGGQPGKH 901

BLAST of Carg27810 vs. NCBI nr
Match: KAF7143693.1 (hypothetical protein RHSIM_Rhsim05G0158900 [Rhododendron simsii])

HSP 1 Score: 683.7 bits (1763), Expect = 1.4e-192
Identity = 393/732 (53.69%), Postives = 466/732 (63.66%), Query Frame = 0

Query: 2   EDKCAIKTDATELIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDRIALSMIQDAED 61
           ++K +I  D TELIG TP+VYLNK+VDGC A++AAKLE MEPCSSVKDRI  SMIQDAED
Sbjct: 51  QEKSSIAKDVTELIGKTPLVYLNKVVDGCVARVAAKLEMMEPCSSVKDRIGYSMIQDAED 110

Query: 62  KGLISPG----------------------KGYRLILIMPAFFSMERRILLLSFGAELHIT 121
           KGLI PG                      KGY+LI+ MPA  SMERRI+LL+FGAEL +T
Sbjct: 111 KGLIKPGESVLIEPTSGNTGIGLAFMAAAKGYKLIITMPASMSMERRIILLAFGAELVLT 170

Query: 122 NPEMSVDAVIKMAEDLEKSTPNGYFLRQFDNPAN-------------------------- 181
           +P   +   ++ AE+++  TPN Y L+QF+NPAN                          
Sbjct: 171 DPSKGMKGAVQKAEEIKAKTPNAYILQQFENPANPKIHYETTGPEIWTRSGGKVDALVCG 230

Query: 182 --------------------AKVYGIEPVESAVLSGGQPGHHLIQGIGAGFIPSILDIKL 241
                                KVYG+EPVESAVLSGG+PG H IQGIGAGFIP +LD  L
Sbjct: 231 IGTGGTVTGAGRYLKEQNPDLKVYGVEPVESAVLSGGKPGPHKIQGIGAGFIPGVLDANL 290

Query: 242 IDEVIQISTEEAIETAKLLASKEGLLVGFSSGAAAAAAIKIAKRPENEGKLIVVIFPKLR 301
           +DEV+QIS+EEA+ETAKLLA KEGLL G SSGAAAAAAIKIAKRPEN GKLIVV+FP   
Sbjct: 291 LDEVVQISSEEAVETAKLLALKEGLLAGISSGAAAAAAIKIAKRPENAGKLIVVVFPSF- 350

Query: 302 RAKFVASKSYFYVILCPF----------------FCSVFLPF-LFPRSLVVYAET----- 361
                  + Y   +LC                  F SV     L  RSL+    T     
Sbjct: 351 ------GERYLSSVLCDSLKKEAEEIYTREWGHEFGSVLDRLDLLLRSLLASKHTKDVVG 410

Query: 362 ------------NLTELMEDKCAIKTDATEVIFTYWAACLFSFPFLDGWFLGLQLIGNTP 421
                          +  ++K AI  D TE                        LIG TP
Sbjct: 411 SRICGGLHCDFGKTPQAAQEKSAIAKDVTE------------------------LIGRTP 470

Query: 422 MVYLNKIVDGCFAKIAAKLESMEPCSSVKDR--------------------VLIEATAGN 481
           +VYLN +VDGC A++AAKLE MEPCSSVKDR                    VLIE T+GN
Sbjct: 471 LVYLNHVVDGCVARVAAKLEMMEPCSSVKDRIGYSMIQDAEEKGLIKPGESVLIEPTSGN 530

Query: 482 TGIGLAWIAAIKGYRLILVMPAFFSMERRILLLAFGAELHVTNPEMSGDAILKIAEDLEK 541
           TGIGLA++AA KGY+LI+ MPA  SMERR +L AFGAEL +T+P    +  +K AE+++ 
Sbjct: 531 TGIGLAFMAAAKGYKLIITMPASMSMERRTILRAFGAELVLTDPAKGVNGAVKKAEEIQA 590

Query: 542 STPNGYFLRQFDNPANTMIHYEATGVEIWKDSNGKVDALVAGIGTGGTLTGAGKFLKEQN 601
            TPN Y L+QF NPAN  IHYE TG EIWK S GKVDALV+GIGTGGT+TGAG++LKE+N
Sbjct: 591 KTPNSYILQQFKNPANPKIHYETTGPEIWKTSGGKVDALVSGIGTGGTVTGAGRYLKEKN 650

Query: 602 PKIKVYGIEPVESAVLSGGQPGR---------HLIQGIGAGIIPSILDVELLDEVIQISS 603
           P IK+YG+EPVESAVLSGG+PG          H IQGIGAG +P +LDV+LLDEVIQISS
Sbjct: 651 PDIKLYGVEPVESAVLSGGEPGEFLFLKCLSPHKIQGIGAGFVPEVLDVQLLDEVIQISS 710

BLAST of Carg27810 vs. ExPASy Swiss-Prot
Match: Q43317 (Cysteine synthase OS=Citrullus lanatus OX=3654 PE=1 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 5.6e-115
Identity = 208/310 (67.10%), Postives = 250/310 (80.65%), Query Frame = 0

Query: 313 QLIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDR--------------------VL 372
           +LIGNTP+VYLN++VDGC A++AAKLE MEPCSSVKDR                    VL
Sbjct: 14  ELIGNTPLVYLNRVVDGCVARVAAKLEMMEPCSSVKDRIGYSMISDAENKGLITPGESVL 73

Query: 373 IEATAGNTGIGLAWIAAIKGYRLILVMPAFFSMERRILLLAFGAELHVTNPEMSGDAILK 432
           IE T+GNTGIGLA+IAA KGYRLI+ MPA  S+ERR +L AFGAEL +T+P       ++
Sbjct: 74  IEPTSGNTGIGLAFIAAAKGYRLIICMPASMSLERRTILRAFGAELVLTDPARGMKGAVQ 133

Query: 433 IAEDLEKSTPNGYFLRQFDNPANTMIHYEATGVEIWKDSNGKVDALVAGIGTGGTLTGAG 492
            AE+++  TPN Y L+QF+NPAN  IHYE TG EIW+ S GK+DALV+GIGTGGT+TGAG
Sbjct: 134 KAEEIKAKTPNSYILQQFENPANPKIHYETTGPEIWRGSGGKIDALVSGIGTGGTVTGAG 193

Query: 493 KFLKEQNPKIKVYGIEPVESAVLSGGQPGRHLIQGIGAGIIPSILDVELLDEVIQISSEE 552
           K+LKEQNP IK+YG+EPVESA+LSGG+PG H IQGIGAG IP +LDV LLDEVIQ+SSEE
Sbjct: 194 KYLKEQNPNIKLYGVEPVESAILSGGKPGPHKIQGIGAGFIPGVLDVNLLDEVIQVSSEE 253

Query: 553 AIETAKLIALKEGLLVGISSGAAAAAAIKVAKRPENEGKLIVVIFPSFGERYLSTSLFDA 603
           +IETAKL+ALKEGLLVGISSGAAAAAAI++AKRPEN GKLIV +FPSFGERYLST LF++
Sbjct: 254 SIETAKLLALKEGLLVGISSGAAAAAAIRIAKRPENAGKLIVAVFPSFGERYLSTVLFES 313

BLAST of Carg27810 vs. ExPASy Swiss-Prot
Match: O81154 (Cysteine synthase OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 2.0e-112
Identity = 202/308 (65.58%), Postives = 247/308 (80.19%), Query Frame = 0

Query: 313 QLIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDR--------------------VL 372
           +LIGNTP+VYLN +VDGC A++AAKLESMEPCSSVKDR                    VL
Sbjct: 14  ELIGNTPLVYLNNVVDGCVARVAAKLESMEPCSSVKDRIGYSMITDAEEKGLIKPGESVL 73

Query: 373 IEATAGNTGIGLAWIAAIKGYRLILVMPAFFSMERRILLLAFGAELHVTNPEMSGDAILK 432
           IE T+GNTG+GLA++AA KGY+LI+ MP+  S+ERRI+L  F +EL +T+P       + 
Sbjct: 74  IEPTSGNTGVGLAFMAAAKGYKLIITMPSSMSLERRIILRGFRSELVLTDPAKGMKGAIS 133

Query: 433 IAEDLEKSTPNGYFLRQFDNPANTMIHYEATGVEIWKDSNGKVDALVAGIGTGGTLTGAG 492
            AE+++  TPN Y L+QF+NPAN  IHYE TG EIWK SNGKVDAL +GIGTGGT+TG+G
Sbjct: 134 KAEEIKAKTPNSYILQQFENPANPKIHYETTGPEIWKGSNGKVDALASGIGTGGTITGSG 193

Query: 493 KFLKEQNPKIKVYGIEPVESAVLSGGQPGRHLIQGIGAGIIPSILDVELLDEVIQISSEE 552
           K+L+EQNP +K+YG+EPVESA+LSGG+PG H IQGIGAG IP +L+V L+D+V+Q+SSEE
Sbjct: 194 KYLREQNPNVKLYGVEPVESAILSGGKPGPHKIQGIGAGFIPGVLEVNLIDDVVQVSSEE 253

Query: 553 AIETAKLIALKEGLLVGISSGAAAAAAIKVAKRPENEGKLIVVIFPSFGERYLSTSLFDA 601
           +IE AKL+ALKEGLLVGISSGAAAAAAIKVAKRPEN GKLIVVIFPSFGERYLS+ LF+ 
Sbjct: 254 SIEMAKLLALKEGLLVGISSGAAAAAAIKVAKRPENAGKLIVVIFPSFGERYLSSVLFET 313

BLAST of Carg27810 vs. ExPASy Swiss-Prot
Match: O23733 (Cysteine synthase OS=Brassica juncea OX=3707 PE=2 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 1.3e-111
Identity = 198/310 (63.87%), Postives = 246/310 (79.35%), Query Frame = 0

Query: 313 QLIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDR--------------------VL 372
           +LIGNTP+VYLN + +GC  ++AAKLE MEPCSSVKDR                    VL
Sbjct: 11  ELIGNTPLVYLNNVAEGCVGRVAAKLEMMEPCSSVKDRIGFSMISDAEQKGLIKPGESVL 70

Query: 373 IEATAGNTGIGLAWIAAIKGYRLILVMPAFFSMERRILLLAFGAELHVTNPEMSGDAILK 432
           IE T+GNTG+GLA+ AA KGY+LI+ MPA  S+ERRI+LLAFG EL +T+P       + 
Sbjct: 71  IEPTSGNTGVGLAFTAAAKGYKLIITMPASMSVERRIILLAFGVELVLTDPAKGMKGAIA 130

Query: 433 IAEDLEKSTPNGYFLRQFDNPANTMIHYEATGVEIWKDSNGKVDALVAGIGTGGTLTGAG 492
            AE++   TPNGY L+QF+NPAN  IHYE TG EIWK ++GK+D  V+GIGTGGT+TGAG
Sbjct: 131 KAEEILAKTPNGYMLQQFENPANPKIHYETTGPEIWKGTDGKIDGFVSGIGTGGTITGAG 190

Query: 493 KFLKEQNPKIKVYGIEPVESAVLSGGQPGRHLIQGIGAGIIPSILDVELLDEVIQISSEE 552
           K+LKEQNP +K+YG+EP+ESA+LSGG+PG H IQGIGAG IPS+L+V+L+DEV+Q+SS+E
Sbjct: 191 KYLKEQNPNVKLYGVEPIESAILSGGKPGPHKIQGIGAGFIPSVLEVDLIDEVVQVSSDE 250

Query: 553 AIETAKLIALKEGLLVGISSGAAAAAAIKVAKRPENEGKLIVVIFPSFGERYLSTSLFDA 603
           +I+ A+L+ALKEGLLVGISSGAAAAAAIK+AKRPEN GKL V +FPSFGERYLST LFDA
Sbjct: 251 SIDMARLLALKEGLLVGISSGAAAAAAIKLAKRPENAGKLFVAVFPSFGERYLSTVLFDA 310

BLAST of Carg27810 vs. ExPASy Swiss-Prot
Match: Q9XEA6 (Cysteine synthase OS=Oryza sativa subsp. japonica OX=39947 GN=RCS1 PE=2 SV=2)

HSP 1 Score: 401.7 bits (1031), Expect = 1.4e-110
Identity = 199/308 (64.61%), Postives = 246/308 (79.87%), Query Frame = 0

Query: 313 QLIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDR--------------------VL 372
           +LIGNTP+VYLN++ DGC  ++AAKLESMEPCSSVKDR                    VL
Sbjct: 11  ELIGNTPLVYLNRVTDGCVGRVAAKLESMEPCSSVKDRIGYSMITDAEEKGLITPGKSVL 70

Query: 373 IEATAGNTGIGLAWIAAIKGYRLILVMPAFFSMERRILLLAFGAELHVTNPEMSGDAILK 432
           IE T+GNTGIGLA++AA KGYRL+L MPA  SMERRI+L AFGAEL +T+P +     ++
Sbjct: 71  IEPTSGNTGIGLAFMAAAKGYRLVLTMPASMSMERRIILKAFGAELILTDPLLGMKGAVQ 130

Query: 433 IAEDLEKSTPNGYFLRQFDNPANTMIHYEATGVEIWKDSNGKVDALVAGIGTGGTLTGAG 492
            AE+L   T N + L+QF+NPAN  IHYE TG EIWK + GKVD LV+GIGTGGT+TGAG
Sbjct: 131 KAEELAAKTNNSFILQQFENPANPKIHYETTGPEIWKGTGGKVDGLVSGIGTGGTITGAG 190

Query: 493 KFLKEQNPKIKVYGIEPVESAVLSGGQPGRHLIQGIGAGIIPSILDVELLDEVIQISSEE 552
           ++L+EQNP IK+YG+EPVESAVLSGG+PG H IQGIGAG +P +LDV+L++E +Q+SS+E
Sbjct: 191 RYLREQNPDIKIYGVEPVESAVLSGGKPGPHKIQGIGAGFVPGVLDVDLINETVQVSSDE 250

Query: 553 AIETAKLIALKEGLLVGISSGAAAAAAIKVAKRPENEGKLIVVIFPSFGERYLSTSLFDA 601
           AIE AK +ALKEGLLVGISSGAAAAAA+++A+RPENEGKL VV+FPSFGERYLS+ LF +
Sbjct: 251 AIEMAKALALKEGLLVGISSGAAAAAAVRLAQRPENEGKLFVVVFPSFGERYLSSVLFQS 310

BLAST of Carg27810 vs. ExPASy Swiss-Prot
Match: Q00834 (Cysteine synthase OS=Spinacia oleracea OX=3562 PE=1 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 1.4e-110
Identity = 201/308 (65.26%), Postives = 242/308 (78.57%), Query Frame = 0

Query: 313 QLIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDR--------------------VL 372
           +LIG TP+VYLN + DGC A++AAKLE MEPCSSVKDR                    VL
Sbjct: 14  ELIGKTPLVYLNTVADGCVARVAAKLEGMEPCSSVKDRIGFSMITDAEKSGLITPGESVL 73

Query: 373 IEATAGNTGIGLAWIAAIKGYRLILVMPAFFSMERRILLLAFGAELHVTNPEMSGDAILK 432
           IE T+GNTGIGLA+IAA KGY+LI+ MPA  S+ERR +L AFGAEL +T+P       ++
Sbjct: 74  IEPTSGNTGIGLAFIAAAKGYKLIITMPASMSLERRTILRAFGAELILTDPAKGMKGAVQ 133

Query: 433 IAEDLEKSTPNGYFLRQFDNPANTMIHYEATGVEIWKDSNGKVDALVAGIGTGGTLTGAG 492
            AE++   TPN Y L+QF+NPAN  +HYE TG EIWK + GK+D  V+GIGTGGT+TGAG
Sbjct: 134 KAEEIRDKTPNSYILQQFENPANPKVHYETTGPEIWKGTGGKIDIFVSGIGTGGTITGAG 193

Query: 493 KFLKEQNPKIKVYGIEPVESAVLSGGQPGRHLIQGIGAGIIPSILDVELLDEVIQISSEE 552
           K+LKEQNP +K+ G+EPVESAVLSGG+PG H IQG+GAG IP +LDV ++DEV+QISSEE
Sbjct: 194 KYLKEQNPDVKLIGLEPVESAVLSGGKPGPHKIQGLGAGFIPGVLDVNIIDEVVQISSEE 253

Query: 553 AIETAKLIALKEGLLVGISSGAAAAAAIKVAKRPENEGKLIVVIFPSFGERYLSTSLFDA 601
           +IE AKL+ALKEGLLVGISSGAAAAAAIKVAKRPEN GKLIV +FPSFGERYLS+ LFD+
Sbjct: 254 SIEMAKLLALKEGLLVGISSGAAAAAAIKVAKRPENAGKLIVAVFPSFGERYLSSVLFDS 313

BLAST of Carg27810 vs. ExPASy TrEMBL
Match: A0A2H5PPZ5 (Cysteine synthase OS=Citrus unshiu OX=55188 GN=CUMW_154590 PE=3 SV=1)

HSP 1 Score: 703.0 bits (1813), Expect = 1.1e-198
Identity = 389/697 (55.81%), Postives = 470/697 (67.43%), Query Frame = 0

Query: 1    MEDKCAIKTDATELIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDRIALSMIQDAE 60
            MEDKC IK D TELIG+TPMVYLN +VDGC A+IAAKLE M+PCSSVKDRIA SMI+DAE
Sbjct: 592  MEDKCEIKKDVTELIGHTPMVYLNNVVDGCVARIAAKLEMMQPCSSVKDRIAYSMIKDAE 651

Query: 61   DKGLISPGK----------------------GYRLILIMPAFFSMERRILLLSFGAELHI 120
            DKGLI+PGK                      GY+LI+IMP+ +S+ERRI+L + GAE+++
Sbjct: 652  DKGLITPGKTVLIELTSGNTGIGLAFIAASRGYKLIIIMPSTYSIERRIILRALGAEVYL 711

Query: 121  TNPEMSVDAVIKMAEDLEKSTPNGYFLRQFDNPA-------------------------- 180
             +P +  +  +K  E++   TPNGY L QF+NPA                          
Sbjct: 712  ADPAVGFEGFVKKGEEILNRTPNGYILGQFENPANPEIHYETTGPEIWNDSGGKVDAFIA 771

Query: 181  --------------------NAKVYGIEPVESAVLSGGQPGHHLIQGIGAGFIPSILDIK 240
                                N KVYGIEP ESAVL+GGQPG HLIQGIGAG IP +LD+ 
Sbjct: 772  GIGTGGTVTGAGRFLKEKNPNIKVYGIEPSESAVLNGGQPGKHLIQGIGAGVIPPVLDVA 831

Query: 241  LIDEVIQISTEEAIETAKLLASKEGLLVGFSSGAAAAAAIKIAKRPENEGKLIVVIFPKL 300
            ++DEVI +S+EEAIET+KLLA KEGLLVG SSGAAAAAAI++AKRPEN GKLIVVIFP  
Sbjct: 832  MLDEVITVSSEEAIETSKLLALKEGLLVGISSGAAAAAAIRVAKRPENAGKLIVVIFPS- 891

Query: 301  RRAKFVASKSYFYVILCPFFCSVFLPFLFPRSLVVYAETNLTELMEDKCAIKTDATEVIF 360
                  A + Y                                 MEDKC IK D TEVIF
Sbjct: 892  ------AGERYLSTAFS---------------------------MEDKCEIKKDVTEVIF 951

Query: 361  TYWAACLFSFPFLDGW---------FLGLQLIGNTPMVYLNKIVDGCFAKIAAKLESMEP 420
             +    L + P + G+         F  +QLIG+TPMVYLN +VDGC A+IAAKLE M+P
Sbjct: 952  -FAQLMLLNLPSVFGYCDLAYRLAVFWLVQLIGHTPMVYLNNVVDGCVARIAAKLEMMQP 1011

Query: 421  CSSVKDR--------------------VLIEATAGNTGIGLAWIAAIKGYRLILVMPAFF 480
            CSSVKDR                    VLIE T+GNTGIGLA+IAA +GY+LI++MP+ +
Sbjct: 1012 CSSVKDRIAYSMVKDAEDKGLITPGKTVLIEITSGNTGIGLAFIAASRGYKLIIIMPSTY 1071

Query: 481  SMERRILLLAFGAELHVTNPEMSGDAILKIAEDLEKSTPNGYFLRQFDNPANTMIHYEAT 540
            S+ERRI+L A GAE+++ +P +  + ++K  E++   TPNGY L QF+NPAN  IHYE T
Sbjct: 1072 SIERRIILRALGAEVYLADPAVGFEGLVKKGEEILNRTPNGYILGQFENPANPEIHYETT 1131

Query: 541  GVEIWKDSNGKVDALVAGIGTGGTLTGAGKFLKEQNPKIKVYGIEPVESAVLSGGQPGRH 600
            G EIW DS GKV A +AGIGTGGT+TGAG+FLKE+NP IKVYGIEP ESA+L+GGQPG+H
Sbjct: 1132 GPEIWNDSGGKVAAFIAGIGTGGTVTGAGRFLKEKNPNIKVYGIEPSESAILNGGQPGKH 1191

BLAST of Carg27810 vs. ExPASy TrEMBL
Match: A0A2H5PP79 (Cysteine synthase OS=Citrus unshiu OX=55188 GN=CUMW_154590 PE=3 SV=1)

HSP 1 Score: 703.0 bits (1813), Expect = 1.1e-198
Identity = 389/697 (55.81%), Postives = 470/697 (67.43%), Query Frame = 0

Query: 1   MEDKCAIKTDATELIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDRIALSMIQDAE 60
           MEDKC IK D TELIG+TPMVYLN +VDGC A+IAAKLE M+PCSSVKDRIA SMI+DAE
Sbjct: 302 MEDKCEIKKDVTELIGHTPMVYLNNVVDGCVARIAAKLEMMQPCSSVKDRIAYSMIKDAE 361

Query: 61  DKGLISPGK----------------------GYRLILIMPAFFSMERRILLLSFGAELHI 120
           DKGLI+PGK                      GY+LI+IMP+ +S+ERRI+L + GAE+++
Sbjct: 362 DKGLITPGKTVLIELTSGNTGIGLAFIAASRGYKLIIIMPSTYSIERRIILRALGAEVYL 421

Query: 121 TNPEMSVDAVIKMAEDLEKSTPNGYFLRQFDNPA-------------------------- 180
            +P +  +  +K  E++   TPNGY L QF+NPA                          
Sbjct: 422 ADPAVGFEGFVKKGEEILNRTPNGYILGQFENPANPEIHYETTGPEIWNDSGGKVDAFIA 481

Query: 181 --------------------NAKVYGIEPVESAVLSGGQPGHHLIQGIGAGFIPSILDIK 240
                               N KVYGIEP ESAVL+GGQPG HLIQGIGAG IP +LD+ 
Sbjct: 482 GIGTGGTVTGAGRFLKEKNPNIKVYGIEPSESAVLNGGQPGKHLIQGIGAGVIPPVLDVA 541

Query: 241 LIDEVIQISTEEAIETAKLLASKEGLLVGFSSGAAAAAAIKIAKRPENEGKLIVVIFPKL 300
           ++DEVI +S+EEAIET+KLLA KEGLLVG SSGAAAAAAI++AKRPEN GKLIVVIFP  
Sbjct: 542 MLDEVITVSSEEAIETSKLLALKEGLLVGISSGAAAAAAIRVAKRPENAGKLIVVIFPS- 601

Query: 301 RRAKFVASKSYFYVILCPFFCSVFLPFLFPRSLVVYAETNLTELMEDKCAIKTDATEVIF 360
                 A + Y                                 MEDKC IK D TEVIF
Sbjct: 602 ------AGERYLSTAFS---------------------------MEDKCEIKKDVTEVIF 661

Query: 361 TYWAACLFSFPFLDGW---------FLGLQLIGNTPMVYLNKIVDGCFAKIAAKLESMEP 420
            +    L + P + G+         F  +QLIG+TPMVYLN +VDGC A+IAAKLE M+P
Sbjct: 662 -FAQLMLLNLPSVFGYCDLAYRLAVFWLVQLIGHTPMVYLNNVVDGCVARIAAKLEMMQP 721

Query: 421 CSSVKDR--------------------VLIEATAGNTGIGLAWIAAIKGYRLILVMPAFF 480
           CSSVKDR                    VLIE T+GNTGIGLA+IAA +GY+LI++MP+ +
Sbjct: 722 CSSVKDRIAYSMVKDAEDKGLITPGKTVLIEITSGNTGIGLAFIAASRGYKLIIIMPSTY 781

Query: 481 SMERRILLLAFGAELHVTNPEMSGDAILKIAEDLEKSTPNGYFLRQFDNPANTMIHYEAT 540
           S+ERRI+L A GAE+++ +P +  + ++K  E++   TPNGY L QF+NPAN  IHYE T
Sbjct: 782 SIERRIILRALGAEVYLADPAVGFEGLVKKGEEILNRTPNGYILGQFENPANPEIHYETT 841

Query: 541 GVEIWKDSNGKVDALVAGIGTGGTLTGAGKFLKEQNPKIKVYGIEPVESAVLSGGQPGRH 600
           G EIW DS GKV A +AGIGTGGT+TGAG+FLKE+NP IKVYGIEP ESA+L+GGQPG+H
Sbjct: 842 GPEIWNDSGGKVAAFIAGIGTGGTVTGAGRFLKEKNPNIKVYGIEPSESAILNGGQPGKH 901

BLAST of Carg27810 vs. ExPASy TrEMBL
Match: A0A2H5PP72 (Cysteine synthase OS=Citrus unshiu OX=55188 GN=CUMW_154590 PE=3 SV=1)

HSP 1 Score: 703.0 bits (1813), Expect = 1.1e-198
Identity = 389/697 (55.81%), Postives = 470/697 (67.43%), Query Frame = 0

Query: 1    MEDKCAIKTDATELIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDRIALSMIQDAE 60
            MEDKC IK D TELIG+TPMVYLN +VDGC A+IAAKLE M+PCSSVKDRIA SMI+DAE
Sbjct: 636  MEDKCEIKKDVTELIGHTPMVYLNNVVDGCVARIAAKLEMMQPCSSVKDRIAYSMIKDAE 695

Query: 61   DKGLISPGK----------------------GYRLILIMPAFFSMERRILLLSFGAELHI 120
            DKGLI+PGK                      GY+LI+IMP+ +S+ERRI+L + GAE+++
Sbjct: 696  DKGLITPGKTVLIELTSGNTGIGLAFIAASRGYKLIIIMPSTYSIERRIILRALGAEVYL 755

Query: 121  TNPEMSVDAVIKMAEDLEKSTPNGYFLRQFDNPA-------------------------- 180
             +P +  +  +K  E++   TPNGY L QF+NPA                          
Sbjct: 756  ADPAVGFEGFVKKGEEILNRTPNGYILGQFENPANPEIHYETTGPEIWNDSGGKVDAFIA 815

Query: 181  --------------------NAKVYGIEPVESAVLSGGQPGHHLIQGIGAGFIPSILDIK 240
                                N KVYGIEP ESAVL+GGQPG HLIQGIGAG IP +LD+ 
Sbjct: 816  GIGTGGTVTGAGRFLKEKNPNIKVYGIEPSESAVLNGGQPGKHLIQGIGAGVIPPVLDVA 875

Query: 241  LIDEVIQISTEEAIETAKLLASKEGLLVGFSSGAAAAAAIKIAKRPENEGKLIVVIFPKL 300
            ++DEVI +S+EEAIET+KLLA KEGLLVG SSGAAAAAAI++AKRPEN GKLIVVIFP  
Sbjct: 876  MLDEVITVSSEEAIETSKLLALKEGLLVGISSGAAAAAAIRVAKRPENAGKLIVVIFPS- 935

Query: 301  RRAKFVASKSYFYVILCPFFCSVFLPFLFPRSLVVYAETNLTELMEDKCAIKTDATEVIF 360
                  A + Y                                 MEDKC IK D TEVIF
Sbjct: 936  ------AGERYLSTAFS---------------------------MEDKCEIKKDVTEVIF 995

Query: 361  TYWAACLFSFPFLDGW---------FLGLQLIGNTPMVYLNKIVDGCFAKIAAKLESMEP 420
             +    L + P + G+         F  +QLIG+TPMVYLN +VDGC A+IAAKLE M+P
Sbjct: 996  -FAQLMLLNLPSVFGYCDLAYRLAVFWLVQLIGHTPMVYLNNVVDGCVARIAAKLEMMQP 1055

Query: 421  CSSVKDR--------------------VLIEATAGNTGIGLAWIAAIKGYRLILVMPAFF 480
            CSSVKDR                    VLIE T+GNTGIGLA+IAA +GY+LI++MP+ +
Sbjct: 1056 CSSVKDRIAYSMVKDAEDKGLITPGKTVLIEITSGNTGIGLAFIAASRGYKLIIIMPSTY 1115

Query: 481  SMERRILLLAFGAELHVTNPEMSGDAILKIAEDLEKSTPNGYFLRQFDNPANTMIHYEAT 540
            S+ERRI+L A GAE+++ +P +  + ++K  E++   TPNGY L QF+NPAN  IHYE T
Sbjct: 1116 SIERRIILRALGAEVYLADPAVGFEGLVKKGEEILNRTPNGYILGQFENPANPEIHYETT 1175

Query: 541  GVEIWKDSNGKVDALVAGIGTGGTLTGAGKFLKEQNPKIKVYGIEPVESAVLSGGQPGRH 600
            G EIW DS GKV A +AGIGTGGT+TGAG+FLKE+NP IKVYGIEP ESA+L+GGQPG+H
Sbjct: 1176 GPEIWNDSGGKVAAFIAGIGTGGTVTGAGRFLKEKNPNIKVYGIEPSESAILNGGQPGKH 1235

BLAST of Carg27810 vs. ExPASy TrEMBL
Match: A0A498IH41 (Cysteine synthase OS=Malus domestica OX=3750 GN=DVH24_036740 PE=3 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 1.1e-187
Identity = 375/704 (53.27%), Postives = 453/704 (64.35%), Query Frame = 0

Query: 1   MEDKCAIKTDATELIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDRIALSMIQDAE 60
           +E+K  I  D TELIG TP+VYLN++VDGC AKIAAKLE MEPCSSVKDRI  SMI+DAE
Sbjct: 18  VEEKSGIAKDVTELIGQTPLVYLNRVVDGCVAKIAAKLEMMEPCSSVKDRIGYSMIKDAE 77

Query: 61  DKGLISPG----------------------KGYRLILIMPAFFSMERRILLLSFGAELHI 120
           +KGLI+PG                      KGY+LI+ MPA  S+ERR +L  FGAEL +
Sbjct: 78  EKGLITPGESVLIEPTSGNTGIGLAFMAAAKGYKLIITMPASMSLERRTILRGFGAELVL 137

Query: 121 TNPEMSVDAVIKMAEDLEKSTPNGYFLRQFDNPANAKV---------------------- 180
           T+P   +   ++ AE++   TPN Y L+QF+NPAN KV                      
Sbjct: 138 TDPARGMKGAVQKAEEIVAKTPNAYMLQQFENPANPKVHYETTGPEIWKGSGGKVDAFVS 197

Query: 181 ------------------------YGIEPVESAVLSGGQPGHHLIQGIGAGFIPSILDIK 240
                                   YG+EPVESAVLSGG+PG H IQGIGAGFIP +LD+ 
Sbjct: 198 GIGTGGTITGVGKYLKEQNPDIKLYGVEPVESAVLSGGKPGPHKIQGIGAGFIPGVLDVN 257

Query: 241 LIDEVIQISTEEAIETAKLLASKEGLLVGFSSGAAAAAAIKIAKRPENEGKLIVVIFPKL 300
           LID+VIQ+++E AIETAKLLA KEGLLVG SSGAAAAAAI+IAKRPEN GKLIVV+FP  
Sbjct: 258 LIDKVIQVTSEVAIETAKLLALKEGLLVGISSGAAAAAAIEIAKRPENAGKLIVVVFPS- 317

Query: 301 RRAKFVASKSYFYVILCPFFCSVFLPFLFPRSLVVYAETNLTELMEDKCAIKTDATEVIF 360
                                              + E  L+ ++ +  ++K +A  +  
Sbjct: 318 -----------------------------------FGERYLSSVLFE--SVKREAESM-- 377

Query: 361 TYWAACLFSFPFLDGWFLGLQLIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDR-- 420
                                LIGNTPMVYLN +VDGC A+IAAKLE+MEPCSSVKDR  
Sbjct: 378 ---------------------LIGNTPMVYLNNVVDGCVARIAAKLETMEPCSSVKDRHQ 437

Query: 421 --------------------------------VLIEATAGNTGIGLAWIAAIKGYRLILV 480
                                           VL+E T+GNTGIGLA++AA KGY+L L 
Sbjct: 438 GMWSLSQILLCRIAYSMIKDAEDKGLITPGETVLVEPTSGNTGIGLAFLAASKGYKLKLS 497

Query: 481 MPAFFSMERRILLLAFGAELHVTNPEMSGDAILKIAEDLEKSTPNGYFLRQFDNPANTMI 540
           MP+  S+ERRI+L AFGAE+++T+P      +   AE+L  +TPN + L QF+NPAN  I
Sbjct: 498 MPSIVSLERRIVLRAFGAEVYITDPAKGIKGVFDKAEELLSNTPNSHMLCQFENPANPKI 557

Query: 541 HYEATGVEIWKDSNGKVDALVAGIGTGGTLTGAGKFLKEQNPKIKVYGIEPVESAVLSGG 600
           HYE TG EIW+DS GKVDALVAGIGTGGT+TGAGKFLKE+N  IKVYG+EP ESAVL+GG
Sbjct: 558 HYETTGPEIWRDSGGKVDALVAGIGTGGTVTGAGKFLKEKNSDIKVYGVEPAESAVLNGG 617

Query: 601 QPGRHLIQGIGAGIIPSILDVELLDEVIQISSEEAIETAKLIALKEGLLVGISSGAAAAA 603
            PG HLIQGIGAG IP +LDV LLDEVIQ+SSEEAI+T+KL+ALKEGLLVGISSGAAAAA
Sbjct: 618 TPGMHLIQGIGAGFIPKVLDVSLLDEVIQVSSEEAIKTSKLLALKEGLLVGISSGAAAAA 660

BLAST of Carg27810 vs. ExPASy TrEMBL
Match: A0A4D6N3F9 (Cysteine synthase OS=Vigna unguiculata OX=3917 GN=DEO72_LG9g2423 PE=3 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 2.1e-186
Identity = 369/681 (54.19%), Postives = 448/681 (65.79%), Query Frame = 0

Query: 1   MEDKCAIKTDATELIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKDRIALSMIQDAE 60
           ME KCAIK D TELIGNTPMVYLN IV+GC A+IAAKLE M+ C SVKDRIALSMI+DAE
Sbjct: 1   MEPKCAIKKDVTELIGNTPMVYLNNIVEGCVARIAAKLEYMQACCSVKDRIALSMIEDAE 60

Query: 61  DKGLISPGK----------------------GYRLILIMPAFFSMERRILLLSFGAELHI 120
            KGLI+PGK                      GY+L + MP++ S+ERRI+L +FGAE+++
Sbjct: 61  KKGLITPGKTVLIEYTSGNTGIGLAFISALRGYKLQVAMPSYVSLERRIILRAFGAEVYL 120

Query: 121 TNPEMSVDAVIKMAEDLEKSTPNGYFLRQFDNPAN------------------------- 180
           T+P   +  VI+  E+L   + +GY L QFDNPAN                         
Sbjct: 121 TDPAKGIAGVIEKGEELLAKSADGYILNQFDNPANPNIHYETTGPEIWRDSGEKIDALVA 180

Query: 181 ---------------------AKVYGIEPVESAVLSGGQPGHHLIQGIGAGFIPSILDIK 240
                                 K+YG+EP ESAVL+GG+PG HLIQG+GAG IP+ILD+ 
Sbjct: 181 GIGTGGSITGAGKFLKEKNPDIKLYGVEPAESAVLNGGKPGKHLIQGLGAGIIPNILDVN 240

Query: 241 LIDEVIQISTEEAIETAKLLASKEGLLVGFSSGAAAAAAIKIAKRPENEGKLIVVIFPKL 300
           ++DEVI+IS+EEAIETAKLLA KEGLL+G SSGAAAAAAIK+ KRPEN GKLIVV     
Sbjct: 241 ILDEVIEISSEEAIETAKLLAVKEGLLMGISSGAAAAAAIKVGKRPENAGKLIVVT---- 300

Query: 301 RRAKFVASKSYFYVILCPFFCSVFLPFLFPRSLVVYAETNLTELMEDKCAIKTDATEVIF 360
                               CS                      ME +CAIK DATE   
Sbjct: 301 --------------------CS------------------RPSNMESQCAIKKDATE--- 360

Query: 361 TYWAACLFSFPFLDGWFLGLQLIGNTPMVYLNKIVDGCFAKIAAKLESMEPCSSVKD--- 420
                                LIGNTPMVYLN IV+GC A+IAAK       S +KD   
Sbjct: 361 ---------------------LIGNTPMVYLNNIVEGCVARIAAK----TAFSMIKDAEE 420

Query: 421 --------RVLIEATAGNTGIGLAWIAAIKGYRLILVMPAFFSMERRILLLAFGAELHVT 480
                    VL+EAT+GNTGIG+A+IA +KGY++I+ MPA  S+ER+I+LLAFGAE+H+T
Sbjct: 421 KGLITPGKSVLVEATSGNTGIGMAFIATLKGYKVIVAMPASVSLERKIVLLAFGAEVHLT 480

Query: 481 NPEMSGDAILKIAEDLEKSTPNGYFLRQFDNPANTMIHYEATGVEIWKDSNGKVDALVAG 540
           +P    D +L+ AE++  +TP  Y LRQ DNPAN  +HYE+TG EIW+DS GKVDALVAG
Sbjct: 481 DPAKGTDEVLRKAEEIVNNTPGSYMLRQIDNPANPKVHYESTGPEIWRDSGGKVDALVAG 540

Query: 541 IGTGGTLTGAGKFLKEQNPKIKVYGIEPVESAVLSGGQPGRHLIQGIGAGIIPSILDVEL 600
           +GTGGT+TGAG+FLKE+NP +K+YG+EP ES VLSGG PG HLIQGIG GIIP++LDV L
Sbjct: 541 VGTGGTITGAGRFLKERNPNVKLYGVEPFESPVLSGGSPGDHLIQGIGNGIIPNVLDVNL 600

Query: 601 LDEVIQISSEEAIETAKLIALKEGLLVGISSGAAAAAAIKVAKRPENEGKLIVVIFPSFG 603
           LDEVIQISS EAIETAKL+ALKEGLL+GISSGAAAAAAIK+ KRPEN GKLIVV+FPS+G
Sbjct: 601 LDEVIQISSVEAIETAKLLALKEGLLMGISSGAAAAAAIKLGKRPENAGKLIVVVFPSYG 611

BLAST of Carg27810 vs. TAIR 10
Match: AT3G04940.1 (cysteine synthase D1 )

HSP 1 Score: 400.6 bits (1028), Expect = 2.2e-111
Identity = 210/343 (61.22%), Postives = 250/343 (72.89%), Query Frame = 0

Query: 278 EDKCAIKTDATEVIFTYWAACLFSFPFLDGWFLGLQLIGNTPMVYLNKIVDGCFAKIAAK 337
           ED+C+IK DAT                        QLIGNTPMVYLN IVDGC A+IAAK
Sbjct: 3   EDRCSIKDDAT------------------------QLIGNTPMVYLNNIVDGCVARIAAK 62

Query: 338 LESMEPCSSVKDRV--------------------LIEATAGNTGIGLAWIAAIKGYRLIL 397
           LE MEPCSSVK+R+                    LIEAT+GNTGIGLA+I A KGY+++L
Sbjct: 63  LEMMEPCSSVKERIAYGMIKDAEDKGLITPGKSTLIEATSGNTGIGLAFIGAAKGYKVVL 122

Query: 398 VMPAFFSMERRILLLAFGAELHVTNPEMSGDAILKIAEDLEKSTPNGYFLRQFDNPANTM 457
            MP+  S+ER+I+LLA GAE+H+T+P      I+  AE++    P+   L QF NP+N  
Sbjct: 123 TMPSSMSLERKIILLALGAEVHLTDPSKGVQGIIDKAEEICSKNPDSIMLEQFKNPSNPQ 182

Query: 458 IHYEATGVEIWKDSNGKVDALVAGIGTGGTLTGAGKFLKEQNPKIKVYGIEPVESAVLSG 517
            HY  TG EIW+DS G+VD LVAG+GTGGTL+G+G+FLKE+N   KVYG+EP ESAV+SG
Sbjct: 183 THYRTTGPEIWRDSAGEVDILVAGVGTGGTLSGSGRFLKEKNKDFKVYGVEPTESAVISG 242

Query: 518 GQPGRHLIQGIGAGIIPSILDVELLDEVIQISSEEAIETAKLIALKEGLLVGISSGAAAA 577
           G+PG HLIQGIGAG+IP  LD  +LDEVIQ++S EAIETAKL+ALKEGLLVGISSGAAAA
Sbjct: 243 GKPGTHLIQGIGAGLIPDNLDFNVLDEVIQVTSVEAIETAKLLALKEGLLVGISSGAAAA 302

Query: 578 AAIKVAKRPENEGKLIVVIFPSFGERYLSTSLFDAARKEAENI 601
           AAIKVAKRPEN GKLIVVIFPS GERYLSTSLF++ R EAEN+
Sbjct: 303 AAIKVAKRPENAGKLIVVIFPSGGERYLSTSLFESVRHEAENL 321

BLAST of Carg27810 vs. TAIR 10
Match: AT5G28020.1 (cysteine synthase D2 )

HSP 1 Score: 396.7 bits (1018), Expect = 3.2e-110
Identity = 211/344 (61.34%), Postives = 247/344 (71.80%), Query Frame = 0

Query: 277 MEDKCAIKTDATEVIFTYWAACLFSFPFLDGWFLGLQLIGNTPMVYLNKIVDGCFAKIAA 336
           MED+C IK D TE                        LIGNTPMVYLN +VDGC A+IAA
Sbjct: 1   MEDRCLIKNDITE------------------------LIGNTPMVYLNNVVDGCVARIAA 60

Query: 337 KLESMEPCSSVKDRV--------------------LIEATAGNTGIGLAWIAAIKGYRLI 396
           KLE MEPCSSVKDR+                    LIE TAGNTGIGLA + A +GY++I
Sbjct: 61  KLEMMEPCSSVKDRIAYSMIKDAEDKGLITPGKSTLIEPTAGNTGIGLACMGAARGYKVI 120

Query: 397 LVMPAFFSMERRILLLAFGAELHVTNPEMSGDAILKIAEDLEKSTPNGYFLRQFDNPANT 456
           LVMP+  S+ERRI+L A GAELH+++  +    +L+  E +   TP GY  +QF+NPAN 
Sbjct: 121 LVMPSTMSLERRIILRALGAELHLSDQRIGLKGMLEKTEAILSKTPGGYIPQQFENPANP 180

Query: 457 MIHYEATGVEIWKDSNGKVDALVAGIGTGGTLTGAGKFLKEQNPKIKVYGIEPVESAVLS 516
            IHY  TG EIW+DS GKVD LVAG+GTGGT TG GKFLKEQN  IKV  +EPVES VLS
Sbjct: 181 EIHYRTTGPEIWRDSAGKVDILVAGVGTGGTATGVGKFLKEQNKDIKVCVVEPVESPVLS 240

Query: 517 GGQPGRHLIQGIGAGIIPSILDVELLDEVIQISSEEAIETAKLIALKEGLLVGISSGAAA 576
           GGQPG HLIQGIG+GI+P  LD+ ++DE+IQ++ EEAIETAKL+ALKEGLLVGISSGAAA
Sbjct: 241 GGQPGPHLIQGIGSGIVPFNLDLTIVDEIIQVAGEEAIETAKLLALKEGLLVGISSGAAA 300

Query: 577 AAAIKVAKRPENEGKLIVVIFPSFGERYLSTSLFDAARKEAENI 601
           AAA+KVAKRPEN GKLIVV+FPS GERYLST LFD+ R EAEN+
Sbjct: 301 AAALKVAKRPENAGKLIVVVFPSGGERYLSTKLFDSIRYEAENL 320

BLAST of Carg27810 vs. TAIR 10
Match: AT5G28020.2 (cysteine synthase D2 )

HSP 1 Score: 396.7 bits (1018), Expect = 3.2e-110
Identity = 211/344 (61.34%), Postives = 247/344 (71.80%), Query Frame = 0

Query: 277 MEDKCAIKTDATEVIFTYWAACLFSFPFLDGWFLGLQLIGNTPMVYLNKIVDGCFAKIAA 336
           MED+C IK D TE                        LIGNTPMVYLN +VDGC A+IAA
Sbjct: 1   MEDRCLIKNDITE------------------------LIGNTPMVYLNNVVDGCVARIAA 60

Query: 337 KLESMEPCSSVKDRV--------------------LIEATAGNTGIGLAWIAAIKGYRLI 396
           KLE MEPCSSVKDR+                    LIE TAGNTGIGLA + A +GY++I
Sbjct: 61  KLEMMEPCSSVKDRIAYSMIKDAEDKGLITPGKSTLIEPTAGNTGIGLACMGAARGYKVI 120

Query: 397 LVMPAFFSMERRILLLAFGAELHVTNPEMSGDAILKIAEDLEKSTPNGYFLRQFDNPANT 456
           LVMP+  S+ERRI+L A GAELH+++  +    +L+  E +   TP GY  +QF+NPAN 
Sbjct: 121 LVMPSTMSLERRIILRALGAELHLSDQRIGLKGMLEKTEAILSKTPGGYIPQQFENPANP 180

Query: 457 MIHYEATGVEIWKDSNGKVDALVAGIGTGGTLTGAGKFLKEQNPKIKVYGIEPVESAVLS 516
            IHY  TG EIW+DS GKVD LVAG+GTGGT TG GKFLKEQN  IKV  +EPVES VLS
Sbjct: 181 EIHYRTTGPEIWRDSAGKVDILVAGVGTGGTATGVGKFLKEQNKDIKVCVVEPVESPVLS 240

Query: 517 GGQPGRHLIQGIGAGIIPSILDVELLDEVIQISSEEAIETAKLIALKEGLLVGISSGAAA 576
           GGQPG HLIQGIG+GI+P  LD+ ++DE+IQ++ EEAIETAKL+ALKEGLLVGISSGAAA
Sbjct: 241 GGQPGPHLIQGIGSGIVPFNLDLTIVDEIIQVAGEEAIETAKLLALKEGLLVGISSGAAA 300

Query: 577 AAAIKVAKRPENEGKLIVVIFPSFGERYLSTSLFDAARKEAENI 601
           AAA+KVAKRPEN GKLIVV+FPS GERYLST LFD+ R EAEN+
Sbjct: 301 AAALKVAKRPENAGKLIVVVFPSGGERYLSTKLFDSIRYEAENL 320

BLAST of Carg27810 vs. TAIR 10
Match: AT5G28020.3 (cysteine synthase D2 )

HSP 1 Score: 396.7 bits (1018), Expect = 3.2e-110
Identity = 211/344 (61.34%), Postives = 247/344 (71.80%), Query Frame = 0

Query: 277 MEDKCAIKTDATEVIFTYWAACLFSFPFLDGWFLGLQLIGNTPMVYLNKIVDGCFAKIAA 336
           MED+C IK D TE                        LIGNTPMVYLN +VDGC A+IAA
Sbjct: 1   MEDRCLIKNDITE------------------------LIGNTPMVYLNNVVDGCVARIAA 60

Query: 337 KLESMEPCSSVKDRV--------------------LIEATAGNTGIGLAWIAAIKGYRLI 396
           KLE MEPCSSVKDR+                    LIE TAGNTGIGLA + A +GY++I
Sbjct: 61  KLEMMEPCSSVKDRIAYSMIKDAEDKGLITPGKSTLIEPTAGNTGIGLACMGAARGYKVI 120

Query: 397 LVMPAFFSMERRILLLAFGAELHVTNPEMSGDAILKIAEDLEKSTPNGYFLRQFDNPANT 456
           LVMP+  S+ERRI+L A GAELH+++  +    +L+  E +   TP GY  +QF+NPAN 
Sbjct: 121 LVMPSTMSLERRIILRALGAELHLSDQRIGLKGMLEKTEAILSKTPGGYIPQQFENPANP 180

Query: 457 MIHYEATGVEIWKDSNGKVDALVAGIGTGGTLTGAGKFLKEQNPKIKVYGIEPVESAVLS 516
            IHY  TG EIW+DS GKVD LVAG+GTGGT TG GKFLKEQN  IKV  +EPVES VLS
Sbjct: 181 EIHYRTTGPEIWRDSAGKVDILVAGVGTGGTATGVGKFLKEQNKDIKVCVVEPVESPVLS 240

Query: 517 GGQPGRHLIQGIGAGIIPSILDVELLDEVIQISSEEAIETAKLIALKEGLLVGISSGAAA 576
           GGQPG HLIQGIG+GI+P  LD+ ++DE+IQ++ EEAIETAKL+ALKEGLLVGISSGAAA
Sbjct: 241 GGQPGPHLIQGIGSGIVPFNLDLTIVDEIIQVAGEEAIETAKLLALKEGLLVGISSGAAA 300

Query: 577 AAAIKVAKRPENEGKLIVVIFPSFGERYLSTSLFDAARKEAENI 601
           AAA+KVAKRPEN GKLIVV+FPS GERYLST LFD+ R EAEN+
Sbjct: 301 AAALKVAKRPENAGKLIVVVFPSGGERYLSTKLFDSIRYEAENL 320

BLAST of Carg27810 vs. TAIR 10
Match: AT5G28020.4 (cysteine synthase D2 )

HSP 1 Score: 396.7 bits (1018), Expect = 3.2e-110
Identity = 211/344 (61.34%), Postives = 247/344 (71.80%), Query Frame = 0

Query: 277 MEDKCAIKTDATEVIFTYWAACLFSFPFLDGWFLGLQLIGNTPMVYLNKIVDGCFAKIAA 336
           MED+C IK D TE                        LIGNTPMVYLN +VDGC A+IAA
Sbjct: 1   MEDRCLIKNDITE------------------------LIGNTPMVYLNNVVDGCVARIAA 60

Query: 337 KLESMEPCSSVKDRV--------------------LIEATAGNTGIGLAWIAAIKGYRLI 396
           KLE MEPCSSVKDR+                    LIE TAGNTGIGLA + A +GY++I
Sbjct: 61  KLEMMEPCSSVKDRIAYSMIKDAEDKGLITPGKSTLIEPTAGNTGIGLACMGAARGYKVI 120

Query: 397 LVMPAFFSMERRILLLAFGAELHVTNPEMSGDAILKIAEDLEKSTPNGYFLRQFDNPANT 456
           LVMP+  S+ERRI+L A GAELH+++  +    +L+  E +   TP GY  +QF+NPAN 
Sbjct: 121 LVMPSTMSLERRIILRALGAELHLSDQRIGLKGMLEKTEAILSKTPGGYIPQQFENPANP 180

Query: 457 MIHYEATGVEIWKDSNGKVDALVAGIGTGGTLTGAGKFLKEQNPKIKVYGIEPVESAVLS 516
            IHY  TG EIW+DS GKVD LVAG+GTGGT TG GKFLKEQN  IKV  +EPVES VLS
Sbjct: 181 EIHYRTTGPEIWRDSAGKVDILVAGVGTGGTATGVGKFLKEQNKDIKVCVVEPVESPVLS 240

Query: 517 GGQPGRHLIQGIGAGIIPSILDVELLDEVIQISSEEAIETAKLIALKEGLLVGISSGAAA 576
           GGQPG HLIQGIG+GI+P  LD+ ++DE+IQ++ EEAIETAKL+ALKEGLLVGISSGAAA
Sbjct: 241 GGQPGPHLIQGIGSGIVPFNLDLTIVDEIIQVAGEEAIETAKLLALKEGLLVGISSGAAA 300

Query: 577 AAAIKVAKRPENEGKLIVVIFPSFGERYLSTSLFDAARKEAENI 601
           AAA+KVAKRPEN GKLIVV+FPS GERYLST LFD+ R EAEN+
Sbjct: 301 AAALKVAKRPENAGKLIVVVFPSGGERYLSTKLFDSIRYEAENL 320