Carg27516 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27516
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncyclin-dependent kinase inhibitor 7-like
LocationCarg_Chr20: 11676210 .. 11677322 (+)
RNA-Seq ExpressionCarg27516
SyntenyCarg27516
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGAGGAAATGCAGAGGAGTAGCAGAGATTGCAGTAATGGAGGTCGACTTAAGGACTACCAGACCTCCACCTGACTTGGATGATGTACCACCTTCCGCCAACAAGAGGAAGATCGCAGCCGAAACAAACTTTGAGTTCTCGACAACGACAACCTCGTTTGTCAAGCTCCGGAGCCGGCGACGCCGCGTTGTTTTGCCGGAGAATTCGGTCCCTGCACCGCCGGCGGAGAATACGCTTCTGCTACCGGGTACAACGTCGACGAAGGAGAGAAGCTCTATTAGCCCGACCTCCGATGATGATGCGTCCACTTTTGCCTGCTCAAGTAACGGCTGCAGCGAGATTGTTGAAGAGAGCAAGTTCGTAGATCTGGAGGTGACAATATTTTGTCTGTTTTTGTGACTTCTGAAGTCTCTAATGTTTTTTATTTGGTTTGTTTCTGAATGTTGTGTGTTCATATTATTTTTCTGCAGGATGAGAAAGATGAACACGGATTCGCGTACAAATCCAGCCGAGAAAGGTTATTAATCTGGCACGAACTTCTTAATTTCCTACTGATCTCGATCTGAAATTGTTGAATCTTCCTTGTACGCGAAAATTTTTAATTCAAAATTCGAAGAAAAAAGTGCTTCAGAAACGGACTTCACCTCCATTAAACTAATTAATTAATTGTTTCCGTTTGTGATTTTAATTCCTCTCTCTCTCTCTCTCGCTCTCACGACTCATAAATCTCCACACTTGTTCTTTCTTTTCATCATCTTGCAGGAGGGAGACGACTCCGTCTAGCCAATTCCGTCCAACACAGTCCGGCGATATGGAGTCTCCGGCAGGGAAAAGCGAGGCAACTTCTTCCGGTAATAGATCTTTGTCTTCGGTGAAGATGCCAGCAGAATCGGAGCTGGAAGCATTCTTCATCGCCGCTGAGAAAAACATACAGAAACGTTTCACTGAGAAGTAAGTTTAATGGAGCTACAGTTTCTTCGAATTTTCCTGTAGCTATGAAAGAAATTAACAGAGTTGGAAAAATCTGAACACAACTGCAGGTATAATTACGATATTATTGAGGATGTTCCCTTGGAAGGACGTTACGAATGGGTTCGATTAAATATTTGA

mRNA sequence

ATGGTGAGGAAATGCAGAGGAGTAGCAGAGATTGCAGTAATGGAGGTCGACTTAAGGACTACCAGACCTCCACCTGACTTGGATGATGTACCACCTTCCGCCAACAAGAGGAAGATCGCAGCCGAAACAAACTTTGAGTTCTCGACAACGACAACCTCGTTTGTCAAGCTCCGGAGCCGGCGACGCCGCGTTGTTTTGCCGGAGAATTCGGTCCCTGCACCGCCGGCGGAGAATACGCTTCTGCTACCGGGTACAACGTCGACGAAGGAGAGAAGCTCTATTAGCCCGACCTCCGATGATGATGCGTCCACTTTTGCCTGCTCAAGTAACGGCTGCAGCGAGATTGTTGAAGAGAGCAAGAGGGAGACGACTCCGTCTAGCCAATTCCGTCCAACACAGTCCGGCGATATGGAGTCTCCGGCAGGGAAAAGCGAGGCAACTTCTTCCGGTAATAGATCTTTGTCTTCGGTGAAGATGCCAGCAGAATCGGAGCTGGAAGCATTCTTCATCGCCGCTGAGAAAAACATACAGAAACGTTTCACTGAGAAGTATAATTACGATATTATTGAGGATGTTCCCTTGGAAGGACGTTACGAATGGGTTCGATTAAATATTTGA

Coding sequence (CDS)

ATGGTGAGGAAATGCAGAGGAGTAGCAGAGATTGCAGTAATGGAGGTCGACTTAAGGACTACCAGACCTCCACCTGACTTGGATGATGTACCACCTTCCGCCAACAAGAGGAAGATCGCAGCCGAAACAAACTTTGAGTTCTCGACAACGACAACCTCGTTTGTCAAGCTCCGGAGCCGGCGACGCCGCGTTGTTTTGCCGGAGAATTCGGTCCCTGCACCGCCGGCGGAGAATACGCTTCTGCTACCGGGTACAACGTCGACGAAGGAGAGAAGCTCTATTAGCCCGACCTCCGATGATGATGCGTCCACTTTTGCCTGCTCAAGTAACGGCTGCAGCGAGATTGTTGAAGAGAGCAAGAGGGAGACGACTCCGTCTAGCCAATTCCGTCCAACACAGTCCGGCGATATGGAGTCTCCGGCAGGGAAAAGCGAGGCAACTTCTTCCGGTAATAGATCTTTGTCTTCGGTGAAGATGCCAGCAGAATCGGAGCTGGAAGCATTCTTCATCGCCGCTGAGAAAAACATACAGAAACGTTTCACTGAGAAGTATAATTACGATATTATTGAGGATGTTCCCTTGGAAGGACGTTACGAATGGGTTCGATTAAATATTTGA

Protein sequence

MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSRRRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESKRETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKMPAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRLNI
Homology
BLAST of Carg27516 vs. NCBI nr
Match: KAG7011200.1 (Cyclin-dependent kinase inhibitor 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 390.6 bits (1002), Expect = 8.5e-105
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0

Query: 1   MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR 60
           MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR
Sbjct: 1   MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR 60

Query: 61  RRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESK 120
           RRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESK
Sbjct: 61  RRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESK 120

Query: 121 RETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKMPAESELEAFFIAAEKNIQKRF 180
           RETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKMPAESELEAFFIAAEKNIQKRF
Sbjct: 121 RETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKMPAESELEAFFIAAEKNIQKRF 180

Query: 181 TEKYNYDIIEDVPLEGRYEWVRLNI 206
           TEKYNYDIIEDVPLEGRYEWVRLNI
Sbjct: 181 TEKYNYDIIEDVPLEGRYEWVRLNI 205

BLAST of Carg27516 vs. NCBI nr
Match: XP_022927984.1 (cyclin-dependent kinase inhibitor 7-like [Cucurbita moschata])

HSP 1 Score: 378.3 bits (970), Expect = 4.3e-101
Identity = 205/226 (90.71%), Postives = 205/226 (90.71%), Query Frame = 0

Query: 1   MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR 60
           MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR
Sbjct: 1   MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR 60

Query: 61  RRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESK 120
           RRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESK
Sbjct: 61  RRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESK 120

Query: 121 ---------------------RETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKM 180
                                RETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKM
Sbjct: 121 FVDLEDEKDEHGFAYKSSRERRETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKM 180

Query: 181 PAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRLNI 206
           PAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRLNI
Sbjct: 181 PAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRLNI 226

BLAST of Carg27516 vs. NCBI nr
Match: XP_023512122.1 (cyclin-dependent kinase inhibitor 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 377.1 bits (967), Expect = 9.7e-101
Identity = 204/226 (90.27%), Postives = 205/226 (90.71%), Query Frame = 0

Query: 1   MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR 60
           MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR
Sbjct: 1   MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR 60

Query: 61  RRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESK 120
           RRRVVLPENSVPAPPAENTLLLPGTTSTKE+SSISPTSDDDASTFACSSNGCSEIVEESK
Sbjct: 61  RRRVVLPENSVPAPPAENTLLLPGTTSTKEKSSISPTSDDDASTFACSSNGCSEIVEESK 120

Query: 121 ---------------------RETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKM 180
                                RETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKM
Sbjct: 121 FVDLEDEKDEHGFAYKSSRERRETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKM 180

Query: 181 PAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRLNI 206
           PAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRLNI
Sbjct: 181 PAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRLNI 226

BLAST of Carg27516 vs. NCBI nr
Match: KAG6571433.1 (Cyclin-dependent kinase inhibitor 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 369.8 bits (948), Expect = 1.5e-98
Identity = 200/226 (88.50%), Postives = 201/226 (88.94%), Query Frame = 0

Query: 1   MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR 60
           MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR
Sbjct: 1   MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR 60

Query: 61  RRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESK 120
           RRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESK
Sbjct: 61  RRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESK 120

Query: 121 ---------------------RETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKM 180
                                RETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKM
Sbjct: 121 FVDLEDEKDEHGFAYKSSRERRETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKM 180

Query: 181 PAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRLNI 206
           PAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEW    +
Sbjct: 181 PAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWTAARV 226

BLAST of Carg27516 vs. NCBI nr
Match: XP_022971737.1 (cyclin-dependent kinase inhibitor 7-like [Cucurbita maxima])

HSP 1 Score: 355.5 bits (911), Expect = 3.0e-94
Identity = 194/226 (85.84%), Postives = 198/226 (87.61%), Query Frame = 0

Query: 1   MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR 60
           MVRKCRGVAEIAV+EVDLRTTRPPPDLDDVPPSANKRKIAA T+FEFSTTTTSFVKLRSR
Sbjct: 1   MVRKCRGVAEIAVIEVDLRTTRPPPDLDDVPPSANKRKIAAGTDFEFSTTTTSFVKLRSR 60

Query: 61  RRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESK 120
           RRRVVLPENSVPAPPA+NTLLLPGTTSTKE SSISPTSDDDASTFACSSNGCSEIVEE K
Sbjct: 61  RRRVVLPENSVPAPPADNTLLLPGTTSTKETSSISPTSDDDASTFACSSNGCSEIVEERK 120

Query: 121 ---------------------RETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKM 180
                                RE TPSSQFRPTQS DMESPAGK EATSSGNRSLSSVKM
Sbjct: 121 FVDLEDEKDEHGFAYKSSRERRERTPSSQFRPTQSSDMESPAGKIEATSSGNRSLSSVKM 180

Query: 181 PAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRLNI 206
           PAESELEAFFIAAEKNIQKRF EKYNYDIIEDVPLEGR+EWVRLNI
Sbjct: 181 PAESELEAFFIAAEKNIQKRFIEKYNYDIIEDVPLEGRHEWVRLNI 226

BLAST of Carg27516 vs. ExPASy Swiss-Prot
Match: Q67J15 (Cyclin-dependent kinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=KRP6 PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 8.2e-10
Identity = 27/52 (51.92%), Postives = 37/52 (71.15%), Query Frame = 0

Query: 152 RSLSSVKMPAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRL 204
           R     + P E E+EAF  AAE ++ +RF  KYNYDI++D P++GRYEWVR+
Sbjct: 33  RKAKKGRSPPEEEVEAFLAAAESSVARRFAAKYNYDIVKDAPMDGRYEWVRV 84

BLAST of Carg27516 vs. ExPASy Swiss-Prot
Match: Q94CL9 (Cyclin-dependent kinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=KRP7 PE=1 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 8.2e-10
Identity = 44/119 (36.97%), Postives = 65/119 (54.62%), Query Frame = 0

Query: 85  TTSTKERSSISPTSDDDASTFACSSNGCSEIVEESKRETTPSSQFRPTQSGDMESPAGKS 144
           ++ T E ++  P SD +A     S    S ++  + R+   SS     ++ +M+S   + 
Sbjct: 77  SSETNEIATRLPFSDLEAHEI--SETEISTLLTNNFRKQGISSSENLGETAEMDSATTEM 136

Query: 145 EATSSGNRSLSSVKMPAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRL 204
                  +     K P ++EL+ FF AAE+  QKRFTEKYNYDI+ D PLEGRY+WV L
Sbjct: 137 RDQRKTEKKKKMEKSPTQAELDDFFSAAERYEQKRFTEKYNYDIVNDTPLEGRYQWVSL 193

BLAST of Carg27516 vs. ExPASy Swiss-Prot
Match: A2X9W8 (Cyclin-dependent kinase inhibitor 1 OS=Oryza sativa subsp. indica OX=39946 GN=KRP1 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.6e-08
Identity = 42/94 (44.68%), Postives = 59/94 (62.77%), Query Frame = 0

Query: 117 EESKRETTPSSQFRPTQSGDMESP-AG--------KSEATSSGNRSLSSVKMPAESELEA 176
           +  +RETTPSS F P +  D+ES  AG         S AT+S  ++ +  K+P  +E+EA
Sbjct: 163 DRERRETTPSS-FLPGEVSDLESDLAGGQKRSRPLPSAATASAQQA-TRPKIPPAAEIEA 222

Query: 177 FFIAAEKNIQKRFTEKYNYDIIEDVPLE-GRYEW 201
           FF AAE+   KRF  KYN+D++  VPL+ GR+EW
Sbjct: 223 FFAAAEEAEAKRFAAKYNFDVVRGVPLDAGRFEW 254

BLAST of Carg27516 vs. ExPASy Swiss-Prot
Match: Q6Z6G5 (Cyclin-dependent kinase inhibitor 1 OS=Oryza sativa subsp. japonica OX=39947 GN=KRP1 PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.6e-08
Identity = 42/94 (44.68%), Postives = 59/94 (62.77%), Query Frame = 0

Query: 117 EESKRETTPSSQFRPTQSGDMESP-AG--------KSEATSSGNRSLSSVKMPAESELEA 176
           +  +RETTPSS F P +  D+ES  AG         S AT+S  ++ +  K+P  +E+EA
Sbjct: 163 DRERRETTPSS-FLPGEVSDLESDLAGGQKRSRPLPSAATASAQQA-TRPKIPPAAEIEA 222

Query: 177 FFIAAEKNIQKRFTEKYNYDIIEDVPLE-GRYEW 201
           FF AAE+   KRF  KYN+D++  VPL+ GR+EW
Sbjct: 223 FFAAAEEAEAKRFAAKYNFDVVRGVPLDAGRFEW 254

BLAST of Carg27516 vs. ExPASy Swiss-Prot
Match: Q9FKB5 (Cyclin-dependent kinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=KRP3 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 5.9e-08
Identity = 64/223 (28.70%), Postives = 104/223 (46.64%), Query Frame = 0

Query: 2   VRKCRGVAEIAVMEVDLRTTRPPPDLDD--VPPSANKRKIAAETNFEFSTTTTSFVKLRS 61
           ++K +   +I+VMEV  + T P P +        A KR  ++  +      ++ +++LRS
Sbjct: 5   MKKSKITGDISVMEVS-KATAPSPGVRTRAAKTLALKRLNSSAADSALPNDSSCYLQLRS 64

Query: 62  RR--RRVVLPENSVPAPPAENTLLLPGTTSTKERSS---ISPTSDDD-------ASTFAC 121
           RR  +   L E   P     + +   G+ S  +  +   ++ +S++D       +   +C
Sbjct: 65  RRLEKPSSLIEPKQPPRVHRSGIKESGSRSRVDSVNSVPVAQSSNEDECFDNFVSVQVSC 124

Query: 122 SSNGCSEIVEESKRETTPSSQFRPTQSGDME-------SPAGKSEATSSGNRSLSSVKMP 181
             N        S RE+TP +        DME       S      AT    R   +V +P
Sbjct: 125 GENSLGFESRHSTRESTPCNFVE-----DMEIMVTPGSSTRSMCRATKEYTREQDNV-IP 184

Query: 182 AESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRL 204
             SE+E FF  AE+  Q+ F EKYN+DI+ D+PL GRYEWV++
Sbjct: 185 TTSEMEEFFAYAEQQQQRLFMEKYNFDIVNDIPLSGRYEWVQV 220

BLAST of Carg27516 vs. ExPASy TrEMBL
Match: A0A6J1EJJ0 (cyclin-dependent kinase inhibitor 7-like OS=Cucurbita moschata OX=3662 GN=LOC111434890 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 2.1e-101
Identity = 205/226 (90.71%), Postives = 205/226 (90.71%), Query Frame = 0

Query: 1   MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR 60
           MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR
Sbjct: 1   MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR 60

Query: 61  RRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESK 120
           RRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESK
Sbjct: 61  RRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESK 120

Query: 121 ---------------------RETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKM 180
                                RETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKM
Sbjct: 121 FVDLEDEKDEHGFAYKSSRERRETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKM 180

Query: 181 PAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRLNI 206
           PAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRLNI
Sbjct: 181 PAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRLNI 226

BLAST of Carg27516 vs. ExPASy TrEMBL
Match: A0A6J1I2S9 (cyclin-dependent kinase inhibitor 7-like OS=Cucurbita maxima OX=3661 GN=LOC111470405 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.5e-94
Identity = 194/226 (85.84%), Postives = 198/226 (87.61%), Query Frame = 0

Query: 1   MVRKCRGVAEIAVMEVDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSR 60
           MVRKCRGVAEIAV+EVDLRTTRPPPDLDDVPPSANKRKIAA T+FEFSTTTTSFVKLRSR
Sbjct: 1   MVRKCRGVAEIAVIEVDLRTTRPPPDLDDVPPSANKRKIAAGTDFEFSTTTTSFVKLRSR 60

Query: 61  RRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESK 120
           RRRVVLPENSVPAPPA+NTLLLPGTTSTKE SSISPTSDDDASTFACSSNGCSEIVEE K
Sbjct: 61  RRRVVLPENSVPAPPADNTLLLPGTTSTKETSSISPTSDDDASTFACSSNGCSEIVEERK 120

Query: 121 ---------------------RETTPSSQFRPTQSGDMESPAGKSEATSSGNRSLSSVKM 180
                                RE TPSSQFRPTQS DMESPAGK EATSSGNRSLSSVKM
Sbjct: 121 FVDLEDEKDEHGFAYKSSRERRERTPSSQFRPTQSSDMESPAGKIEATSSGNRSLSSVKM 180

Query: 181 PAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRLNI 206
           PAESELEAFFIAAEKNIQKRF EKYNYDIIEDVPLEGR+EWVRLNI
Sbjct: 181 PAESELEAFFIAAEKNIQKRFIEKYNYDIIEDVPLEGRHEWVRLNI 226

BLAST of Carg27516 vs. ExPASy TrEMBL
Match: A0A6J1L6D4 (cyclin-dependent kinase inhibitor 7-like OS=Cucurbita maxima OX=3661 GN=LOC111499589 PE=3 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 9.6e-62
Identity = 147/232 (63.36%), Postives = 163/232 (70.26%), Query Frame = 0

Query: 1   MVRKCRGVAEIAVMEVDL-RTTRPPPDLDDVP-----PSANKRKIAAETNFEFSTTTTSF 60
           MVRKCR VAEIAVMEVD+ RTTRPPPDL+DVP      SANKR+I A T+F FST TTSF
Sbjct: 1   MVRKCRAVAEIAVMEVDVRRTTRPPPDLEDVPHSSTSTSANKRQITAGTDFHFSTATTSF 60

Query: 61  VKLRSRRRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSE 120
           V+L  RRRR+VL        P E++L  P TTST+ERSS SP SDDDAS F CSSNGCSE
Sbjct: 61  VRLPRRRRRLVL--------PVESSLRRPSTTSTEERSSTSPISDDDASAFCCSSNGCSE 120

Query: 121 IVEES-----------------------KRETTPSSQFRPTQSGDMESPAGKSEATSSGN 180
           +VEES                       +RETTPSS F+ T SGDMES A K+ ATS   
Sbjct: 121 VVEESSKFVDLEDEEKDEHGIAYKSSRERRETTPSSHFQSTMSGDMESLAVKTNATSGNV 180

Query: 181 RSLSSVKMPAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRL 204
            S SS+K PAESELE FF AAEK IQKRF EKYNYDI+EDVPLEGRYEW+RL
Sbjct: 181 SSSSSMKKPAESELEEFFTAAEKKIQKRFAEKYNYDIVEDVPLEGRYEWIRL 224

BLAST of Carg27516 vs. ExPASy TrEMBL
Match: A0A6J1G8D3 (cyclin-dependent kinase inhibitor 7-like OS=Cucurbita moschata OX=3662 GN=LOC111451687 PE=3 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 2.1e-61
Identity = 146/232 (62.93%), Postives = 163/232 (70.26%), Query Frame = 0

Query: 1   MVRKCRGVAEIAVMEVDL-RTTRPPPDLDDVP-----PSANKRKIAAETNFEFSTTTTSF 60
           MVRKCR VAEIAVMEVD+ RTTRPPPDL+DVP      SANKR+I A T+F F+T TTSF
Sbjct: 1   MVRKCRAVAEIAVMEVDVRRTTRPPPDLEDVPHSSTNTSANKRQITAGTDFHFTTATTSF 60

Query: 61  VKLRSRRRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSE 120
           VKL  RRRR+VL        P E++L  P TTST+ERSS SP SDDDAS F CSSNGCSE
Sbjct: 61  VKLPRRRRRLVL--------PLESSLRQPSTTSTEERSSTSPISDDDASAFCCSSNGCSE 120

Query: 121 IVEES-----------------------KRETTPSSQFRPTQSGDMESPAGKSEATSSGN 180
           +VEES                       +RETTPSS F+ T S DMES A K++ATS   
Sbjct: 121 VVEESSKFVDLEDEEKDEHGIAYKSCRERRETTPSSHFQSTMSSDMESLAMKTKATSGNG 180

Query: 181 RSLSSVKMPAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRL 204
            S SS+K PAESELE FF AAEK IQKRF EKYNYDI+EDVPLEGRYEW+RL
Sbjct: 181 SSSSSMKKPAESELEEFFTAAEKKIQKRFAEKYNYDIVEDVPLEGRYEWIRL 224

BLAST of Carg27516 vs. ExPASy TrEMBL
Match: A0A6J1D6U1 (cyclin-dependent kinase inhibitor 7-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017879 PE=3 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 3.2e-57
Identity = 144/237 (60.76%), Postives = 164/237 (69.20%), Query Frame = 0

Query: 1   MVRKCRGVAEIAVMEVDLRTTR---PPPDLDDVPPS-ANKRKIAAETN-FEFSTTTTSFV 60
           MV+KCRGVAEI+VME+DLRTTR   P  D DD+P S +NKRKI A T+ F+FST TTSF+
Sbjct: 1   MVKKCRGVAEISVMELDLRTTRAAQPDLDDDDLPASTSNKRKITAGTDLFQFSTATTSFL 60

Query: 61  KLRS-RRRRVVLPENSVPAPPAENTLLLPGTTSTKERSSISPTSDDDASTFAC-SSNGCS 120
           KLRS RRRR++LPENSV   P E T+L    T     SS +P SDDDAS   C SSNGCS
Sbjct: 61  KLRSRRRRRILLPENSVATAPPETTVLRQPRT-----SSTTPCSDDDASAICCSSSNGCS 120

Query: 121 EIVEES---------------------------KRETTPSSQFRPTQSGDMESPAGKSEA 180
           E+VEES                           +RETTPSS+FR  QS DMESPAGKSEA
Sbjct: 121 EVVEESRVIKFVDLEDENNDELGVRAYENRSRERRETTPSSKFRSAQSDDMESPAGKSEA 180

Query: 181 TSSGNRSLSSVKMPAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRL 204
           TS    SLS  KMP ES+LE FFI+AEKN+QK F EKYNYDI+EDVPLEGRYEW+RL
Sbjct: 181 TSGDTSSLSVAKMPTESDLEEFFISAEKNLQKCFAEKYNYDIVEDVPLEGRYEWIRL 232

BLAST of Carg27516 vs. TAIR 10
Match: AT1G49620.1 (Cyclin-dependent kinase inhibitor family protein )

HSP 1 Score: 65.5 bits (158), Expect = 5.8e-11
Identity = 44/119 (36.97%), Postives = 65/119 (54.62%), Query Frame = 0

Query: 85  TTSTKERSSISPTSDDDASTFACSSNGCSEIVEESKRETTPSSQFRPTQSGDMESPAGKS 144
           ++ T E ++  P SD +A     S    S ++  + R+   SS     ++ +M+S   + 
Sbjct: 77  SSETNEIATRLPFSDLEAHEI--SETEISTLLTNNFRKQGISSSENLGETAEMDSATTEM 136

Query: 145 EATSSGNRSLSSVKMPAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRL 204
                  +     K P ++EL+ FF AAE+  QKRFTEKYNYDI+ D PLEGRY+WV L
Sbjct: 137 RDQRKTEKKKKMEKSPTQAELDDFFSAAERYEQKRFTEKYNYDIVNDTPLEGRYQWVSL 193

BLAST of Carg27516 vs. TAIR 10
Match: AT5G48820.1 (inhibitor/interactor with cyclin-dependent kinase )

HSP 1 Score: 59.3 bits (142), Expect = 4.2e-09
Identity = 64/223 (28.70%), Postives = 104/223 (46.64%), Query Frame = 0

Query: 2   VRKCRGVAEIAVMEVDLRTTRPPPDLDD--VPPSANKRKIAAETNFEFSTTTTSFVKLRS 61
           ++K +   +I+VMEV  + T P P +        A KR  ++  +      ++ +++LRS
Sbjct: 5   MKKSKITGDISVMEVS-KATAPSPGVRTRAAKTLALKRLNSSAADSALPNDSSCYLQLRS 64

Query: 62  RR--RRVVLPENSVPAPPAENTLLLPGTTSTKERSS---ISPTSDDD-------ASTFAC 121
           RR  +   L E   P     + +   G+ S  +  +   ++ +S++D       +   +C
Sbjct: 65  RRLEKPSSLIEPKQPPRVHRSGIKESGSRSRVDSVNSVPVAQSSNEDECFDNFVSVQVSC 124

Query: 122 SSNGCSEIVEESKRETTPSSQFRPTQSGDME-------SPAGKSEATSSGNRSLSSVKMP 181
             N        S RE+TP +        DME       S      AT    R   +V +P
Sbjct: 125 GENSLGFESRHSTRESTPCNFVE-----DMEIMVTPGSSTRSMCRATKEYTREQDNV-IP 184

Query: 182 AESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRL 204
             SE+E FF  AE+  Q+ F EKYN+DI+ D+PL GRYEWV++
Sbjct: 185 TTSEMEEFFAYAEQQQQRLFMEKYNFDIVNDIPLSGRYEWVQV 220

BLAST of Carg27516 vs. TAIR 10
Match: AT5G48820.2 (inhibitor/interactor with cyclin-dependent kinase )

HSP 1 Score: 57.4 bits (137), Expect = 1.6e-08
Identity = 37/90 (41.11%), Postives = 49/90 (54.44%), Query Frame = 0

Query: 121 RETTPSSQFRPTQSGDME-------SPAGKSEATSSGNRSLSSVKMPAESELEAFFIAAE 180
           RE+TP +        DME       S      AT    R   +V +P  SE+E FF  AE
Sbjct: 155 RESTPCNFVE-----DMEIMVTPGSSTRSMCRATKEYTREQDNV-IPTTSEMEEFFAYAE 214

Query: 181 KNIQKRFTEKYNYDIIEDVPLEGRYEWVRL 204
           +  Q+ F EKYN+DI+ D+PL GRYEWV++
Sbjct: 215 QQQQRLFMEKYNFDIVNDIPLSGRYEWVQV 238

BLAST of Carg27516 vs. TAIR 10
Match: AT2G23430.1 (Cyclin-dependent kinase inhibitor family protein )

HSP 1 Score: 54.7 bits (130), Expect = 1.0e-07
Identity = 51/177 (28.81%), Postives = 86/177 (48.59%), Query Frame = 0

Query: 51  TTSFVKLRSRRRRVVLPENSVPAPPAEN---TLLLPGTTSTKERSSI-----SPTSDDDA 110
           ++++++LRSRR   V  E S       +   +    G+   K++  I         D + 
Sbjct: 17  SSTYMQLRSRRIVYVRSEKSSSVSVVGDNGVSSSCSGSNEYKKKELIHLEEEDKDGDTET 76

Query: 111 STFACSSNG--CSEIVEESKRETTPSSQFRPTQSGDMESPAGKS-EATSSGNRSLSS--- 170
           ST+   +       + EE K E + S +     S + ES   +S +   SG +++     
Sbjct: 77  STYRRGTKRKLFENLREEEKEELSKSME---NYSSEFESAVKESLDCCCSGRKTMEETVT 136

Query: 171 ----------VKMPAESELEAFFIAAEKNIQKRFTEKYNYDIIEDVPLEGRYEWVRL 204
                      +MP ESE+E FF+ AEK ++++F +KYN+D  ++ PLEGRYEWV+L
Sbjct: 137 AEEEEKAKLMTEMPTESEIEDFFVEAEKQLKEKFKKKYNFDFEKEKPLEGRYEWVKL 190

BLAST of Carg27516 vs. TAIR 10
Match: AT3G24810.1 (Cyclin-dependent kinase inhibitor family protein )

HSP 1 Score: 52.4 bits (124), Expect = 5.1e-07
Identity = 56/193 (29.02%), Postives = 84/193 (43.52%), Query Frame = 0

Query: 16  VDLRTTRPPPDLDDVPPSANKRKIAAETNFEFSTTTTSFVKLRSRRRRVV-LPENSVPAP 75
           V ++    PP L     +A  + +A       S    SF  L+ R RR+V LP       
Sbjct: 14  VSVKDKSHPPALGFRTRAAAAKNLALHRLRSHSDEADSFNYLQLRSRRLVKLP------- 73

Query: 76  PAENTLLLPGTTSTKERSSISPTSDDDASTFACSSNGCSEIVEESKRETTPSSQFRPTQS 135
                 LL  T   +++  I   +         SS    ++  ++  E       R ++S
Sbjct: 74  ------LLTNTRKQQKQQLIPSVNQCQTKNPRASSGPAKKLEPDTTTEEACGDNERISRS 133

Query: 136 ----GDMESPAGKSEATSSGNRSLSSVKMPAESELEAFFIAAEKNIQKRFTEKYNYDIIE 195
               GD      K     S NRS+ S     +SE+E FF +AE+  Q+ F +KYN+DI+ 
Sbjct: 134 DCNFGD------KGFDLESENRSMISDSKSIQSEIEDFFASAEQQQQRFFIQKYNFDIVS 187

Query: 196 DVPLEGRYEWVRL 204
           D PL GRYEWV++
Sbjct: 194 DNPLPGRYEWVKV 187

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7011200.18.5e-105100.00Cyclin-dependent kinase inhibitor 7, partial [Cucurbita argyrosperma subsp. argy... [more]
XP_022927984.14.3e-10190.71cyclin-dependent kinase inhibitor 7-like [Cucurbita moschata][more]
XP_023512122.19.7e-10190.27cyclin-dependent kinase inhibitor 7-like [Cucurbita pepo subsp. pepo][more]
KAG6571433.11.5e-9888.50Cyclin-dependent kinase inhibitor 7, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_022971737.13.0e-9485.84cyclin-dependent kinase inhibitor 7-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q67J158.2e-1051.92Cyclin-dependent kinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q94CL98.2e-1036.97Cyclin-dependent kinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=KRP7 PE=1... [more]
A2X9W82.6e-0844.68Cyclin-dependent kinase inhibitor 1 OS=Oryza sativa subsp. indica OX=39946 GN=KR... [more]
Q6Z6G52.6e-0844.68Cyclin-dependent kinase inhibitor 1 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q9FKB55.9e-0828.70Cyclin-dependent kinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=KRP3 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1EJJ02.1e-10190.71cyclin-dependent kinase inhibitor 7-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1I2S91.5e-9485.84cyclin-dependent kinase inhibitor 7-like OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
A0A6J1L6D49.6e-6263.36cyclin-dependent kinase inhibitor 7-like OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
A0A6J1G8D32.1e-6162.93cyclin-dependent kinase inhibitor 7-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1D6U13.2e-5760.76cyclin-dependent kinase inhibitor 7-like isoform X2 OS=Momordica charantia OX=36... [more]
Match NameE-valueIdentityDescription
AT1G49620.15.8e-1136.97Cyclin-dependent kinase inhibitor family protein [more]
AT5G48820.14.2e-0928.70inhibitor/interactor with cyclin-dependent kinase [more]
AT5G48820.21.6e-0841.11inhibitor/interactor with cyclin-dependent kinase [more]
AT2G23430.11.0e-0728.81Cyclin-dependent kinase inhibitor family protein [more]
AT3G24810.15.1e-0729.02Cyclin-dependent kinase inhibitor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044275Cyclin-dependent kinase inhibitor, plantPIRSFPIRSF017811CKI_KRP_plantcoord: 1..205
e-value: 3.3E-34
score: 117.0
IPR044275Cyclin-dependent kinase inhibitor, plantPANTHERPTHR46776CYCLIN-DEPENDENT KINASE INHIBITOR 4-RELATEDcoord: 3..204
IPR003175Cyclin-dependent kinase inhibitor domainPFAMPF02234CDIcoord: 160..203
e-value: 4.1E-16
score: 58.9
IPR044898Cyclin-dependent kinase inhibitor domain superfamilyGENE3D4.10.365.10p27coord: 148..205
e-value: 5.3E-9
score: 37.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 80..114
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 125..158
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 66..158
NoneNo IPR availablePANTHERPTHR46776:SF9CYCLIN-DEPENDENT KINASE INHIBITOR 7coord: 3..204

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27516-RACarg27516-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045736 negative regulation of cyclin-dependent protein serine/threonine kinase activity
biological_process GO:0051726 regulation of cell cycle
cellular_component GO:0005654 nucleoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0004861 cyclin-dependent protein serine/threonine kinase inhibitor activity