Carg27380 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27380
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	malignant T-cell-amplified sequence 1 homolog
Location	Carg_Chr10: 9286323 .. 9288374 (+)
RNA-Seq Expression	Carg27380
Synteny	Carg27380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTTCTCTCGACGTCTGCGTACTCTCTTCCCATTTCCACACCGATCCTCTGGTGGGATCCGAGCAATTGTCTCCTATCGTTCTGGAGTCCTCTTCAAATTCAATCTAACAATCTGTATTTTCTGAAATTTTTATTAGGATTCTCTTCATATAACTCTGCGGCATCATGTTCAAAAAGTGAGTTCTCTCTATATTGCTCGTTATTCGGCTATGATTTCATGTCTTTGCTTGATGATTGTGGAAAATGATATCTAGGTTTTGTTTTTCGGGGATTAAAATATGAGGAAATGGTTTGATTTCTTCACTTTTTGTTACTGGTCTCTGGATTTTGCTCGATTAGTGTCTTCTCTCTCGTTCTACGCTGGGGATTTGTTTGTAGGGTTGATTCTTCTCATAGGAAAAGAGGATTGTTTTGCTTCTGTTTTTCGATTGATTGTTTTGCCCTAGATTTCTACTTGCCGAGAGTCGTTATCGTTGTCGTAATGTGTCATAATGTGCGGAAATAATCATTTGAAACTTCGATATAAGACATAAACTTCATCTTAAAACACTGTCATGAGCTTACTTGTTCGAAAACATCTTTAAACCGACACAACGAAATACTAAATGCAATAAATAAATATTAGAATTAATGCAACTATCCTATGCACGTGTCATGGTTTCGAATTGCGATGTCGTCGTTAGCCGTAGAGGCCTTGCCTTTACTTGAAAATGATTGTTTTGCTTCTATTTTTCGTTTGATTGTTTGCCCTAGATTTCTATTTGTCGAGAGTTGCTATCGTCGTCATAATGTGCAGAAATAATCATTTGAAACTTTGACATAAGACATAAACTTCATCTTAAAACACTGTCATGGACTTACATGTTCGAAAACATCTTTAAAACGACTCAACGAAATACTAAATGAAATAAATAGACATTAGAATTAAAAACATCTTATCCTAACCTAAGACTAAGGAAATAAATGCAACTGCCCTATGCATGTGTCATGGTCTCGAGTTGCGATATCATTGTTACCCGTACAGGGATGCCTTGCCTTTCCCTGAACAAATGATTATTTTGCTTCTATTTTTTCGTTTGATTCTTTTGGGCAGCTGTGGATAAATCATTGTTGCTGAATTTGAACATTGAATTGAAATGATAGTTTTGGAGTTGGGATGTTTTCTTGTGGAGTTGAGTGAGTGTGTTGTTTGTACAAATCTTTCAATTGTGTTCTTTTTGCTGTCTTATTGTAGGTTTTCTACTGAAGACGTGTCTGCCCAGAATCAAGTCAAGGCATCGGTGCAACGGAGGATTCGGCAAAGCATAGCTGAAGAAGTAAGCATTGGTTTAACCGTTCGAATCTCGGAATCATGTTCATTAGATAGCAACTGATATCTACCTGTTTTTTGTCTTTTGATTGCAGTATCCTGGACTGGAGCCAGTTTTGGATGATTTGCTTCCTAAAAAAGCTCCTCTGATTGTAACTAAGTGGTTAGAACTTCTGTTTGCTTCTATTTTTGCAGAAAATGATGGATGTTCTTTGTTTTTCTTTGTTAATTTGATCATATGAGGCTTATGTTGGATTTTGTTTTTGGATTTATAGTCAAAACCATCTCAATCTGGTTGTTGTGAACAATGTGCCACTCTTTTTCAATATCCGTGATGGGCCCTACATGCCTACATTAAGGTTGCTTCATCAATGTAATGTTCTTCTGATAACCCTTTTAACTTCTGTTGCAATATGTATCATTTTTGTGGATCTTTTTTCTTAAACATGTAAGCCCATTGCATTGTTTTGTGAAGATGTTTTAAATGAATATTTCCGCACAAGATGTTATGTCATGCATGTAGTAGTGACATTAGATTAGTAGAAAACTAGGGTCGTTACAATATTCTTCTGACAACCCTTGTAACTTCTATTGCAATACATATCGTAGGTGTTGAGGATTGTTGGGAGGGAGTCCCACATTGACTAATTAAGAGGACAATCCTTGGTTTGTAAGTAAGGAATATATCTCCATTGGTGGGGGAGGCCTTTTGGGGAAACCAAAAGCAAAACCACGAGAGTTAT

mRNA sequence

TCTTCTCTCGACGTCTGCGTACTCTCTTCCCATTTCCACACCGATCCTCTGGTGGGATCCGAGCAATTGTCTCCTATCGTTCTGGAGTCCTCTTCAAATTCAATCTAACAATCTGTATTTTCTGAAATTTTTATTAGGATTCTCTTCATATAACTCTGCGGCATCATGTTCAAAAAGTTTTCTACTGAAGACGTGTCTGCCCAGAATCAAGTCAAGGCATCGGTGCAACGGAGGATTCGGCAAAGCATAGCTGAAGAATATCCTGGACTGGAGCCAGTTTTGGATGATTTGCTTCCTAAAAAAGCTCCTCTGATTTCAAAACCATCTCAATCTGGTTGTTGTGAACAATGTGCCACTCTTTTTCAATATCCGTGATGGGCCCTACATGCCTACATTAAGGTTGCTTCATCAATGTAATGTTCTTCTGATAACCCTTTTAACTTCTGTTGCAATATGTATCATTTTTGTGGATCTTTTTTCTTAAACATGTAAGCCCATTGCATTGTTTTGTGAAGATGTTTTAAATGAATATTTCCGCACAAGATGTTATGTCATGCATGTAGTAGTGACATTAGATTAGTAGAAAACTAGGGTCGTTACAATATTCTTCTGACAACCCTTGTAACTTCTATTGCAATACATATCGTAGGTGTTGAGGATTGTTGGGAGGGAGTCCCACATTGACTAATTAAGAGGACAATCCTTGGTTTGTAAGTAAGGAATATATCTCCATTGGTGGGGGAGGCCTTTTGGGGAAACCAAAAGCAAAACCACGAGAGTTAT

Coding sequence (CDS)

ATGTTCAAAAAGTTTTCTACTGAAGACGTGTCTGCCCAGAATCAAGTCAAGGCATCGGTGCAACGGAGGATTCGGCAAAGCATAGCTGAAGAATATCCTGGACTGGAGCCAGTTTTGGATGATTTGCTTCCTAAAAAAGCTCCTCTGATTTCAAAACCATCTCAATCTGGTTGTTGTGAACAATGTGCCACTCTTTTTCAATATCCGTGA

Protein sequence

MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLISKPSQSGCCEQCATLFQYP

Homology

[Close]

BLAST of Carg27380 vs. NCBI nr
Match: KAG7023988.1 (TMA20 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 141.4 bits (355), Expect = 3.0e-30
Identity = 69/69 (100.00%), Postives = 69/69 (100.00%), Query Frame = 0


Query: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLISKPSQSGCCE 60
          MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLISKPSQSGCCE
Sbjct: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLISKPSQSGCCE 60

Query: 61 QCATLFQYP 70
          QCATLFQYP
Sbjct: 61 QCATLFQYP 69

[Close]

BLAST of Carg27380 vs. NCBI nr
Match: KAG2256652.1 (hypothetical protein Bca52824_075946 [Brassica carinata])

HSP 1 Score: 99.8 bits (247), Expect = 1.0e-17
Identity = 50/71 (70.42%), Postives = 56/71 (78.87%), Query Frame = 0


Query: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLI-----SKPSQ 60
          MFKKFS E+VS+QNQVK SVQRRIRQSI +EYPGLE V+DDLLPKK+PLI      KPS 
Sbjct: 1  MFKKFSLEEVSSQNQVKTSVQRRIRQSIQDEYPGLETVMDDLLPKKSPLIVVKWFPKPSY 60

Query: 61 SGCCEQCATLF 67
            CC+QCA  F
Sbjct: 61 PSCCQQCALFF 71

[Close]

BLAST of Carg27380 vs. NCBI nr
Match: XP_022968801.1 (malignant T-cell-amplified sequence 1 homolog [Cucurbita maxima])

HSP 1 Score: 99.0 bits (245), Expect = 1.7e-17
Identity = 51/56 (91.07%), Postives = 52/56 (92.86%), Query Frame = 0


Query: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLISKPSQS 57
          MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLI    Q+
Sbjct: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLIVTKCQN 56

[Close]

BLAST of Carg27380 vs. NCBI nr
Match: XP_023515958.1 (malignant T-cell-amplified sequence 1 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 99.0 bits (245), Expect = 1.7e-17
Identity = 51/56 (91.07%), Postives = 52/56 (92.86%), Query Frame = 0


Query: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLISKPSQS 57
          MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLI    Q+
Sbjct: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLIVTKCQN 56

[Close]

BLAST of Carg27380 vs. NCBI nr
Match: XP_022961538.1 (malignant T-cell-amplified sequence 1 homolog [Cucurbita moschata] >KAG6590453.1 Malignant T-cell-amplified sequence 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 99.0 bits (245), Expect = 1.7e-17
Identity = 51/56 (91.07%), Postives = 52/56 (92.86%), Query Frame = 0


Query: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLISKPSQS 57
          MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLI    Q+
Sbjct: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLIVTKCQN 56

[Close]

BLAST of Carg27380 vs. ExPASy Swiss-Prot
Match: Q75AA8 (Translation machinery-associated protein 20 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=TMA20 PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 4.4e-08
Identity = 26/49 (53.06%), Postives = 36/49 (73.47%), Query Frame = 0


Query: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPL 50
          MFKKFS ED+  + +VK SVQR ++  + E++P LE V+D+LLPKK  L
Sbjct: 1  MFKKFSKEDIHTRTKVKTSVQRSLKSKLVEQFPKLEEVIDELLPKKTEL 49

[Close]

BLAST of Carg27380 vs. ExPASy Swiss-Prot
Match: P89886 (Translation machinery-associated protein 20 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TMA20 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 2.2e-07
Identity = 22/49 (44.90%), Postives = 40/49 (81.63%), Query Frame = 0


Query: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPL 50
          MFKKF+ EDV ++++VK+S+QR ++  + ++YP +E V+D+L+PKK+ +
Sbjct: 1  MFKKFTREDVHSRSKVKSSIQRTLKAKLVKQYPKIEDVIDELIPKKSQI 49

[Close]

BLAST of Carg27380 vs. ExPASy Swiss-Prot
Match: Q86KL4 (Malignant T-cell-amplified sequence 1 homolog OS=Dictyostelium discoideum OX=44689 GN=mcts1 PE=3 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 2.0e-05
Identity = 24/57 (42.11%), Postives = 37/57 (64.91%), Query Frame = 0


Query: 1  MFKKFSTEDVS-AQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLISKPSQS 57
          MFKK S +  S   NQ+K+S+QR I+ SI  +YP L+ + +D+ PKK P++    Q+
Sbjct: 1  MFKKLSLKGSSNTNNQIKSSLQRNIKNSILAQYPKLKDIEEDVFPKKVPIVQVKCQN 57

[Close]

BLAST of Carg27380 vs. ExPASy Swiss-Prot
Match: P87313 (Translation machinery-associated protein 20 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tma20 PE=3 SV=5)

HSP 1 Score: 48.9 bits (115), Expect = 2.7e-05
Identity = 22/50 (44.00%), Postives = 35/50 (70.00%), Query Frame = 0


Query: 1  MFKKF-STEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPL 50
          MFK+F S ED+     +K+S+QR I+  + + YP L+ V+D+L+PKK+ L
Sbjct: 1  MFKRFNSREDIKGTTPIKSSIQRGIKAKLVQAYPNLKQVIDELIPKKSQL 50

[Close]

BLAST of Carg27380 vs. ExPASy Swiss-Prot
Match: Q2KIE4 (Malignant T-cell-amplified sequence 1 OS=Bos taurus OX=9913 GN=MCTS1 PE=2 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 2.9e-04
Identity = 23/50 (46.00%), Postives = 35/50 (70.00%), Query Frame = 0


Query: 1  MFKKF-STEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPL 50
          MFKKF   E+VS   Q+K SV + I+  + E++PG+EP L+ ++PKK P+
Sbjct: 1  MFKKFDEKENVSNCIQLKTSVIKGIKNQLIEQFPGIEPWLNQIMPKKDPV 50

[Close]

BLAST of Carg27380 vs. ExPASy TrEMBL
Match: A0A6J1HVW2 (malignant T-cell-amplified sequence 1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111467930 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 8.3e-18
Identity = 51/56 (91.07%), Postives = 52/56 (92.86%), Query Frame = 0


Query: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLISKPSQS 57
          MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLI    Q+
Sbjct: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLIVTKCQN 56

[Close]

BLAST of Carg27380 vs. ExPASy TrEMBL
Match: A0A6J1HC33 (malignant T-cell-amplified sequence 1 homolog OS=Cucurbita moschata OX=3662 GN=LOC111462086 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 8.3e-18
Identity = 51/56 (91.07%), Postives = 52/56 (92.86%), Query Frame = 0


Query: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLISKPSQS 57
          MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLI    Q+
Sbjct: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLIVTKCQN 56

[Close]

BLAST of Carg27380 vs. ExPASy TrEMBL
Match: A0A0A0LYF6 (PUA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569260 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.8e-17
Identity = 50/56 (89.29%), Postives = 52/56 (92.86%), Query Frame = 0


Query: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLISKPSQS 57
          MFKKFSTEDVSAQNQVKASVQR+IRQSIAEEYPGLEPVLDDLLPKKAPLI    Q+
Sbjct: 1  MFKKFSTEDVSAQNQVKASVQRKIRQSIAEEYPGLEPVLDDLLPKKAPLIVTKCQN 56

[Close]

BLAST of Carg27380 vs. ExPASy TrEMBL
Match: B3TLK5 (Pseudouridine synthase and archaeosine transglycosylase-like protein OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.8e-17
Identity = 50/56 (89.29%), Postives = 52/56 (92.86%), Query Frame = 0


Query: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLISKPSQS 57
          MFKKFSTEDVSAQNQVKASVQR+IRQSIAEEYPGLEPVLDDLLPKKAPLI    Q+
Sbjct: 1  MFKKFSTEDVSAQNQVKASVQRKIRQSIAEEYPGLEPVLDDLLPKKAPLIVTKCQN 56

[Close]

BLAST of Carg27380 vs. ExPASy TrEMBL
Match: A0A6J1C273 (malignant T-cell-amplified sequence 1 homolog OS=Momordica charantia OX=3673 GN=LOC111007697 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.8e-17
Identity = 50/56 (89.29%), Postives = 52/56 (92.86%), Query Frame = 0


Query: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLISKPSQS 57
          MFKKFSTEDVSAQNQVKASVQR+IRQSIAEEYPGLEPVLDDLLPKKAPLI    Q+
Sbjct: 1  MFKKFSTEDVSAQNQVKASVQRKIRQSIAEEYPGLEPVLDDLLPKKAPLIVTKCQN 56

[Close]

BLAST of Carg27380 vs. TAIR 10
Match: AT1G09150.1 (pseudouridine synthase and archaeosine transglycosylase (PUA) domain-containing protein )

HSP 1 Score: 80.9 bits (198), Expect = 4.5e-16
Identity = 39/50 (78.00%), Postives = 45/50 (90.00%), Query Frame = 0


Query: 1  MFKKFSTEDVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDLLPKKAPLI 51
          MFKKF  E++S+QNQVKASVQRRIRQSI +EYPGLE V++DLLPKK PLI
Sbjct: 1  MFKKFCLEEISSQNQVKASVQRRIRQSIQDEYPGLESVMEDLLPKKIPLI 50

The following BLAST results are available for this feature:

BLAST of Carg27380 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7023988.1	3.0e-30	100.00	TMA20 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG2256652.1	1.0e-17	70.42	hypothetical protein Bca52824_075946 [Brassica carinata]	[more]
XP_022968801.1	1.7e-17	91.07	malignant T-cell-amplified sequence 1 homolog [Cucurbita maxima]	[more]
XP_023515958.1	1.7e-17	91.07	malignant T-cell-amplified sequence 1 homolog [Cucurbita pepo subsp. pepo]	[more]
XP_022961538.1	1.7e-17	91.07	malignant T-cell-amplified sequence 1 homolog [Cucurbita moschata] >KAG6590453.1...	[more]

BLAST of Carg27380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q75AA8	4.4e-08	53.06	Translation machinery-associated protein 20 OS=Ashbya gossypii (strain ATCC 1089...	[more]
P89886	2.2e-07	44.90	Translation machinery-associated protein 20 OS=Saccharomyces cerevisiae (strain ...	[more]
Q86KL4	2.0e-05	42.11	Malignant T-cell-amplified sequence 1 homolog OS=Dictyostelium discoideum OX=446...	[more]
P87313	2.7e-05	44.00	Translation machinery-associated protein 20 OS=Schizosaccharomyces pombe (strain...	[more]
Q2KIE4	2.9e-04	46.00	Malignant T-cell-amplified sequence 1 OS=Bos taurus OX=9913 GN=MCTS1 PE=2 SV=1	[more]

BLAST of Carg27380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HVW2	8.3e-18	91.07	malignant T-cell-amplified sequence 1 homolog OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1HC33	8.3e-18	91.07	malignant T-cell-amplified sequence 1 homolog OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A0A0LYF6	1.8e-17	89.29	PUA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569260 PE=4 SV...	[more]
B3TLK5	1.8e-17	89.29	Pseudouridine synthase and archaeosine transglycosylase-like protein OS=Cucumis ...	[more]
A0A6J1C273	1.8e-17	89.29	malignant T-cell-amplified sequence 1 homolog OS=Momordica charantia OX=3673 GN=...	[more]

BLAST of Carg27380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G09150.1	4.5e-16	78.00	pseudouridine synthase and archaeosine transglycosylase (PUA) domain-containing ...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR041366	Pre-PUA domain	PFAM	PF17832	Pre-PUA	coord: 7..50 e-value: 8.8E-11 score: 42.3
None	No IPR available	GENE3D	3.10.400.20		coord: 1..66 e-value: 2.7E-7 score: 32.4
None	No IPR available	PANTHER	PTHR22798:SF9	BNACNNG06600D PROTEIN	coord: 1..51
IPR016437	MCT-1/Tma20	PANTHER	PTHR22798	MCT-1 PROTEIN	coord: 1..51

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27380-RA	Carg27380-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001731	formation of translation preinitiation complex
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0003723	RNA binding