Carg27230 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27230
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	Carg_Chr05: 6422191 .. 6424997 (+)
RNA-Seq Expression	Carg27230
Synteny	Carg27230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATAGGCTTAACTCACTTGTTACTGAGAAGTGAGTGTCGCACAATTACAAGTCTGCTGCCATCAAAGTGCTATTGTGACAACGAGCAGGGTAGAGAAGTCAGTCATGGGGGACTCAAGAGTTTTATGTTTTTATTTTTATTTATTTTTTTATTGATAGACGTTTCCACACTTAGTTGTTCATTGGTTTAAACTGTGTTTGTGGACATCTAGTTTATAGGGACTACGGAGTGACACCGAGTTAACTCGTGGTTTTAAGTTGTTTGATTAAATTTTTTTAGGTTGCTCACCTTTTAACCTTTTTAAAGGGAACCCGCACCTGATTTTAACAAATTACCTCTTTTGCATATTTCATTTGTTTTAAAAGTCTTAAAATCACTTTTCTTTGTAACATCCATGACTGCTATGGGTAAGAAAAGTTGTCTTTGCATATGATTTTTCATTTATGTATTTTAAGTTTTAAGATTTTTTTATTTATTTCTAGGTTACATTCCCAGTTTCTAATTATGATGATAAGGTATAAAGATTACAAACTCTTGGAATACCTCAAGTAAAGTCAAAGGTTTCATTTTGAGGTTATAAAAACGCATATATGTAAATTAATAACGACACACTATCAAAGTAAATTAATAACGAGGAATGCTTTCATAATGGAATGAGTTATCATAAATATATAAAATTAAAAATTTATGAATTTATTTATAAAAATTAATTCTAAAGCATGAGAACTTGGGTATTATTGTTAAAACTACAAATAAGATGATAGGTTACTATAAATAATTGTATTTTAACAGGTATATTCAAGAGCTTCTGCCATCGACAACCGATCCTGCATTGAAGAATGACCTCATAGTTTGTGAAAATGCATACAAGACACTGAGTGAAGCATTTCAAGAAGGAATTGAGTTATTCTTCAAAAAGGATTATAGAAGCATGTTGAATGCCGAGAAGGTTGCACCGAGAGCACAGGCGACCACCCGAGAAACAAAGCCCACTAGAGGAGAGGAACAGGGAGATGAGGATTCTCATTGCCGTAGCTATAGTTTCCGGCTCCATGATTGCATCTTAACGACCCTACTCTTAACTCTATCTAGACTCTTTTGGAATTTAAAATGCCTGTTTCTCTTTTTCAACAAATCTTTATTTTGAAAGATTTATATTAAAACATATAATAGAAAGTAATATTTTTTTAGAAAATATTAAAAGACGGAGAAAGTTCTTTAATTATTTAATTTTTAACAAAATAAGTTATTGCAGTGTAGTGCACACTAAAATGTTTCTCGTGCCTAGGATTCATATCGATTACCTATTTGTCTTGAAGTGTTTCTTAGTTATCCTACATAGCATTACTCTAAAGGCTCCTATATTTGTTGTATCTTATATGGTATATGAAGACACGTTTAACCTTACCGTTAGTATGATTGAGCCACCACAAAGAAATATGCACTTTATTGGTATAGATATTAACTATCAATTTTAGTATGCATTTCTTAACCATTATATCCAAAATCGTTCTCATTTCGGTATGCTCTTGGTTCATTCATAAATCTCTCATTTAAGCATTAAATTTATTCGACTAAAACGACTATGTTCGTTGTACCAAAAAAAAAAAAAATTGCATATGGTTGTATCTTTTATCTTCTTGCCAAAAAGTGTTACTTCTTTGAATTAAAGGTATATAAGAGCAAAGATGGAAGTAATAAAGAGTCGTATTGAATTAATTGAGTTATATTTAAATTTATATGTTATGGACGCTTTGAACGAAGTATTTAGCTTCGAATTTGAGCTAATATAACGGTCGTTTAAGCCATTGAAATTCCCATTTCTAAAACTAATACGATTGCTAGTTTTTCCGAACTAACTTAAGAAGGTTATTTGGTATTTATTATATTAAAAATAGAATTGTTGGATGAAGGTCAGTTTTCACATAGTTTAACAAATAAGATTTTAATTATTATTTCAAAGGCCATAATTTGATTCTCTTACTCTCACAATAGTGTTAAACTAACAAAATCCAACTTAAAAAGAGACTATTTATGGAAAATGGAAGGGAAATAAAGGGAGAGAAGTGAGCATTATTGGATAACTCGAGCTACCCATTGCTAAAGACGGTCCCATAAGATAGACATTCATTCCCCTTCACTTTGTCAAAATCCAATATGGTTTTTACAAGATGTTTTTCCGTCTTCTCTGATGTTGCTTGTCTTGATCGTTGTCTTCTTTATTGTTCAATCAGTAGTGGGTGACAGTGACACTGAGGATAGGATCAATAACTTGTGTCATCAAATGGAGGAATTCGGGTTTTGCAGTCAAACCTTCCATGAGAATCTAAATGGTCCGACAGACGATATAGGACTAACCCAAATAGCAATTTTCCAAGCTCAAGCCAATGCAAGCAAAACACTTCGGTATTGTTGTGAAAAATACATATCAAATTTAAAAAGTTACAAAGGGTTTAAGTTGATAGGTTAAGATGAATACTTGCATTCTTAACAGGTATATTCAAGAGCTTCTACCAACAATAACTGATCCTGCATTGAAGAACGGCCTTATAGTATGTGAAAATGCATACAAGACAGTGAATGAAGCACTTCAAGAAGGGATTGAATTATTCTTCAAAAAAGATTATAGAAGCATGTTAAATGTCGAGAAGATTGCACCCAGAGCACAAGCGAGTTGCAACATGATTTTTAGCACACCACCCGAAAAACAAGGTCTACTAGAGGAGAGGAACAGGGAGATAAGAATTCTGATTGCCATGGCTATAGTTTCGGGCTACATCATGACCACTTCTACCTCTCTCTAA

mRNA sequence

ATGAATAGGCTTAACTCACTTGTTACTGAGAAGTATATTCAAGAGCTTCTGCCATCGACAACCGATCCTGCATTGAAGAATGACCTCATAGTTTGTGAAAATGCATACAAGACACTGAGTGAAGCATTTCAAGAAGGAATTGAGTTATTCTTCAAAAAGGATTATAGAAGCATGTTGAATGCCGAGAAGGTTGCACCGAGAGCACAGGCGACCACCCGAGAAACAAAGCCCACTAGAGGAGAGGAACAGGGAGATGAGGATTCTCATTGCCTAGTGGGTGACAGTGACACTGAGGATAGGATCAATAACTTGTGTCATCAAATGGAGGAATTCGGGTTTTGCAGTCAAACCTTCCATGAGAATCTAAATGGTCCGACAGACGATATAGGACTAACCCAAATAGCAATTTTCCAAGCTCAAGCCAATGCAAGCAAAACACTTCGGTATATTCAAGAGCTTCTACCAACAATAACTGATCCTGCATTGAAGAACGGCCTTATAGTATGTGAAAATGCATACAAGACAGTGAATGAAGCACTTCAAGAAGGGATTGAATTATTCTTCAAAAAAGATTATAGAAGCATGTTAAATGTCGAGAAGATTGCACCCAGAGCACAAGCGAGTTGCAACATGATTTTTAGCACACCACCCGAAAAACAAGGTCTACTAGAGGAGAGGAACAGGGAGATAAGAATTCTGATTGCCATGGCTATAGTTTCGGGCTACATCATGACCACTTCTACCTCTCTCTAA

Coding sequence (CDS)

ATGAATAGGCTTAACTCACTTGTTACTGAGAAGTATATTCAAGAGCTTCTGCCATCGACAACCGATCCTGCATTGAAGAATGACCTCATAGTTTGTGAAAATGCATACAAGACACTGAGTGAAGCATTTCAAGAAGGAATTGAGTTATTCTTCAAAAAGGATTATAGAAGCATGTTGAATGCCGAGAAGGTTGCACCGAGAGCACAGGCGACCACCCGAGAAACAAAGCCCACTAGAGGAGAGGAACAGGGAGATGAGGATTCTCATTGCCTAGTGGGTGACAGTGACACTGAGGATAGGATCAATAACTTGTGTCATCAAATGGAGGAATTCGGGTTTTGCAGTCAAACCTTCCATGAGAATCTAAATGGTCCGACAGACGATATAGGACTAACCCAAATAGCAATTTTCCAAGCTCAAGCCAATGCAAGCAAAACACTTCGGTATATTCAAGAGCTTCTACCAACAATAACTGATCCTGCATTGAAGAACGGCCTTATAGTATGTGAAAATGCATACAAGACAGTGAATGAAGCACTTCAAGAAGGGATTGAATTATTCTTCAAAAAAGATTATAGAAGCATGTTAAATGTCGAGAAGATTGCACCCAGAGCACAAGCGAGTTGCAACATGATTTTTAGCACACCACCCGAAAAACAAGGTCTACTAGAGGAGAGGAACAGGGAGATAAGAATTCTGATTGCCATGGCTATAGTTTCGGGCTACATCATGACCACTTCTACCTCTCTCTAA

Protein sequence

MNRLNSLVTEKYIQELLPSTTDPALKNDLIVCENAYKTLSEAFQEGIELFFKKDYRSMLNAEKVAPRAQATTRETKPTRGEEQGDEDSHCLVGDSDTEDRINNLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAIFQAQANASKTLRYIQELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCNMIFSTPPEKQGLLEERNREIRILIAMAIVSGYIMTTSTSL

Homology

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BLAST of Carg27230 vs. NCBI nr
Match: KAG7029966.1 (hypothetical protein SDJN02_08310, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 495.7 bits (1275), Expect = 2.3e-136
Identity = 250/250 (100.00%), Postives = 250/250 (100.00%), Query Frame = 0


Query: 1   MNRLNSLVTEKYIQELLPSTTDPALKNDLIVCENAYKTLSEAFQEGIELFFKKDYRSMLN 60
           MNRLNSLVTEKYIQELLPSTTDPALKNDLIVCENAYKTLSEAFQEGIELFFKKDYRSMLN
Sbjct: 1   MNRLNSLVTEKYIQELLPSTTDPALKNDLIVCENAYKTLSEAFQEGIELFFKKDYRSMLN 60

Query: 61  AEKVAPRAQATTRETKPTRGEEQGDEDSHCLVGDSDTEDRINNLCHQMEEFGFCSQTFHE 120
           AEKVAPRAQATTRETKPTRGEEQGDEDSHCLVGDSDTEDRINNLCHQMEEFGFCSQTFHE
Sbjct: 61  AEKVAPRAQATTRETKPTRGEEQGDEDSHCLVGDSDTEDRINNLCHQMEEFGFCSQTFHE 120

Query: 121 NLNGPTDDIGLTQIAIFQAQANASKTLRYIQELLPTITDPALKNGLIVCENAYKTVNEAL 180
           NLNGPTDDIGLTQIAIFQAQANASKTLRYIQELLPTITDPALKNGLIVCENAYKTVNEAL
Sbjct: 121 NLNGPTDDIGLTQIAIFQAQANASKTLRYIQELLPTITDPALKNGLIVCENAYKTVNEAL 180

Query: 181 QEGIELFFKKDYRSMLNVEKIAPRAQASCNMIFSTPPEKQGLLEERNREIRILIAMAIVS 240
           QEGIELFFKKDYRSMLNVEKIAPRAQASCNMIFSTPPEKQGLLEERNREIRILIAMAIVS
Sbjct: 181 QEGIELFFKKDYRSMLNVEKIAPRAQASCNMIFSTPPEKQGLLEERNREIRILIAMAIVS 240

Query: 241 GYIMTTSTSL 251
           GYIMTTSTSL
Sbjct: 241 GYIMTTSTSL 250

[Close]

BLAST of Carg27230 vs. NCBI nr
Match: KAG6599000.1 (hypothetical protein SDJN03_08778, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 315.8 bits (808), Expect = 3.2e-82
Identity = 157/160 (98.12%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 91  LVGDSDTEDRINNLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAIFQAQANASKTLRYI 150
           +VGDSDTEDRINNLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAIFQAQANASKTLRYI
Sbjct: 15  VVGDSDTEDRINNLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAIFQAQANASKTLRYI 74

Query: 151 QELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCN 210
           QELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCN
Sbjct: 75  QELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCN 134

Query: 211 MIFSTPPEKQGLLEERNREIRILIAMAIVSGYIMTTSTSL 251
           MIFSTPPEKQGLLEERNREIRILIAMAIVSGYIMT+S+SL
Sbjct: 135 MIFSTPPEKQGLLEERNREIRILIAMAIVSGYIMTSSSSL 174

[Close]

BLAST of Carg27230 vs. NCBI nr
Match: XP_022946902.1 (uncharacterized protein LOC111450843 [Cucurbita moschata])

HSP 1 Score: 313.9 bits (803), Expect = 1.2e-81
Identity = 156/160 (97.50%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 91  LVGDSDTEDRINNLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAIFQAQANASKTLRYI 150
           +VGDSDTEDRI+NLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAIFQAQANASKTLRYI
Sbjct: 26  VVGDSDTEDRIDNLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAIFQAQANASKTLRYI 85

Query: 151 QELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCN 210
           QELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCN
Sbjct: 86  QELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCN 145

Query: 211 MIFSTPPEKQGLLEERNREIRILIAMAIVSGYIMTTSTSL 251
           MIFSTPPEKQGLLEERNREIRILIAMAIVSGYIMT+S+SL
Sbjct: 146 MIFSTPPEKQGLLEERNREIRILIAMAIVSGYIMTSSSSL 185

[Close]

BLAST of Carg27230 vs. NCBI nr
Match: XP_022972818.1 (uncharacterized protein LOC111471342 [Cucurbita maxima])

HSP 1 Score: 300.1 bits (767), Expect = 1.8e-77
Identity = 148/160 (92.50%), Postives = 155/160 (96.88%), Query Frame = 0


Query: 91  LVGDSDTEDRINNLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAIFQAQANASKTLRYI 150
           +V DS+TE+RINNLCHQMEEFGFCSQTFHENLNGPTDD+ LTQIAIFQAQAN SKTLRYI
Sbjct: 22  IVADSETENRINNLCHQMEEFGFCSQTFHENLNGPTDDVELTQIAIFQAQANTSKTLRYI 81

Query: 151 QELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCN 210
           QELLPTITDPALKN L+VCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCN
Sbjct: 82  QELLPTITDPALKNNLLVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCN 141

Query: 211 MIFSTPPEKQGLLEERNREIRILIAMAIVSGYIMTTSTSL 251
           MIF+TPPEKQGLLEERNREIRILIAMAIVSGYIMT S+SL
Sbjct: 142 MIFNTPPEKQGLLEERNREIRILIAMAIVSGYIMTGSSSL 181

[Close]

BLAST of Carg27230 vs. NCBI nr
Match: XP_023546425.1 (uncharacterized protein LOC111805547 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 255.8 bits (652), Expect = 4.0e-64
Identity = 125/155 (80.65%), Postives = 140/155 (90.32%), Query Frame = 0


Query: 92  VGDSDTEDRINNLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAIFQAQANASKTLRYIQ 151
           + D++TE+RINNLCH+MEEFGFCSQTFHENLNGPTDDIGLTQIAI +AQANAS TLRYIQ
Sbjct: 49  IADAETENRINNLCHEMEEFGFCSQTFHENLNGPTDDIGLTQIAIVEAQANASNTLRYIQ 108

Query: 152 ELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCNM 211
           ELLPTITD  LKN LI+CENAYKTV+EALQEGI LFFK+DYR ML   KIAPRAQASC+M
Sbjct: 109 ELLPTITDLKLKNDLIICENAYKTVDEALQEGIGLFFKRDYRGMLIAVKIAPRAQASCDM 168

Query: 212 IFSTPPEKQGLLEERNREIRILIAMAIVSGYIMTT 247
           IFSTP EK+GLLEERNRE+RIL+AMAIV G I+ +
Sbjct: 169 IFSTPLEKRGLLEERNREVRILVAMAIVCGSIIVS 203

[Close]

BLAST of Carg27230 vs. ExPASy TrEMBL
Match: A0A6J1G4X2 (uncharacterized protein LOC111450843 OS=Cucurbita moschata OX=3662 GN=LOC111450843 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 5.9e-82
Identity = 156/160 (97.50%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 91  LVGDSDTEDRINNLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAIFQAQANASKTLRYI 150
           +VGDSDTEDRI+NLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAIFQAQANASKTLRYI
Sbjct: 26  VVGDSDTEDRIDNLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAIFQAQANASKTLRYI 85

Query: 151 QELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCN 210
           QELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCN
Sbjct: 86  QELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCN 145

Query: 211 MIFSTPPEKQGLLEERNREIRILIAMAIVSGYIMTTSTSL 251
           MIFSTPPEKQGLLEERNREIRILIAMAIVSGYIMT+S+SL
Sbjct: 146 MIFSTPPEKQGLLEERNREIRILIAMAIVSGYIMTSSSSL 185

[Close]

BLAST of Carg27230 vs. ExPASy TrEMBL
Match: A0A6J1IB75 (uncharacterized protein LOC111471342 OS=Cucurbita maxima OX=3661 GN=LOC111471342 PE=4 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 8.9e-78
Identity = 148/160 (92.50%), Postives = 155/160 (96.88%), Query Frame = 0


Query: 91  LVGDSDTEDRINNLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAIFQAQANASKTLRYI 150
           +V DS+TE+RINNLCHQMEEFGFCSQTFHENLNGPTDD+ LTQIAIFQAQAN SKTLRYI
Sbjct: 22  IVADSETENRINNLCHQMEEFGFCSQTFHENLNGPTDDVELTQIAIFQAQANTSKTLRYI 81

Query: 151 QELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCN 210
           QELLPTITDPALKN L+VCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCN
Sbjct: 82  QELLPTITDPALKNNLLVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCN 141

Query: 211 MIFSTPPEKQGLLEERNREIRILIAMAIVSGYIMTTSTSL 251
           MIF+TPPEKQGLLEERNREIRILIAMAIVSGYIMT S+SL
Sbjct: 142 MIFNTPPEKQGLLEERNREIRILIAMAIVSGYIMTGSSSL 181

[Close]

BLAST of Carg27230 vs. ExPASy TrEMBL
Match: A0A6J1KFT2 (uncharacterized protein LOC111493503 OS=Cucurbita maxima OX=3661 GN=LOC111493503 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 2.5e-64
Identity = 126/155 (81.29%), Postives = 140/155 (90.32%), Query Frame = 0


Query: 92  VGDSDTEDRINNLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAIFQAQANASKTLRYIQ 151
           + D++TE+RINNLCH+MEEFGFCSQTFHENLNGPTDDIGLTQIAI +AQANAS TLRYIQ
Sbjct: 1   MADTETENRINNLCHEMEEFGFCSQTFHENLNGPTDDIGLTQIAIVEAQANASNTLRYIQ 60

Query: 152 ELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCNM 211
           ELLPTITD  LKN LI+CENAYKTV+EALQEGI LFFKKDYR ML   KIAPRAQASC+M
Sbjct: 61  ELLPTITDLKLKNDLIICENAYKTVDEALQEGIGLFFKKDYRGMLIAVKIAPRAQASCDM 120

Query: 212 IFSTPPEKQGLLEERNREIRILIAMAIVSGYIMTT 247
           IFSTP +K+GLLEERNREIRIL+AMAIV G I+ +
Sbjct: 121 IFSTPVKKRGLLEERNREIRILVAMAIVCGSIIVS 155

[Close]

BLAST of Carg27230 vs. ExPASy TrEMBL
Match: A0A6J1G4V3 (uncharacterized protein LOC111450827 OS=Cucurbita moschata OX=3662 GN=LOC111450827 PE=4 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 1.8e-62
Identity = 122/155 (78.71%), Postives = 138/155 (89.03%), Query Frame = 0


Query: 92  VGDSDTEDRINNLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAIFQAQANASKTLRYIQ 151
           + D++TE++INNLCH+MEEFGFCSQTFHENLNGPTDDIGLTQIAI +AQANAS TLRYIQ
Sbjct: 1   MADTETENKINNLCHEMEEFGFCSQTFHENLNGPTDDIGLTQIAIVEAQANASNTLRYIQ 60

Query: 152 ELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCNM 211
           ELLPTI D  LKN LI+CENAYKTV+EALQEGI LF K+DYR ML   KIAPRAQASC+M
Sbjct: 61  ELLPTIIDLKLKNDLIICENAYKTVDEALQEGIGLFLKRDYRGMLIAVKIAPRAQASCDM 120

Query: 212 IFSTPPEKQGLLEERNREIRILIAMAIVSGYIMTT 247
           IFSTP EK+GLLEERNRE+RIL+AMAIV G I+ +
Sbjct: 121 IFSTPLEKRGLLEERNREVRILVAMAIVCGSIIVS 155

[Close]

BLAST of Carg27230 vs. ExPASy TrEMBL
Match: A0A6J1DRX6 (uncharacterized protein LOC111023283 OS=Momordica charantia OX=3673 GN=LOC111023283 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 9.3e-51
Identity = 100/149 (67.11%), Postives = 124/149 (83.22%), Query Frame = 0


Query: 92  VGDSDTEDRINNLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAIFQAQANASKTLRYIQ 151
           +GD +TE+RIN +C QMEEFGFCSQTFHENL GPTD + LTQIA  +A  NA++TLRYI 
Sbjct: 27  LGDPETEERINRVCRQMEEFGFCSQTFHENLKGPTDYVRLTQIAKVEAIINATRTLRYIT 86

Query: 152 ELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCNM 211
           +LL  ++DPALKN LIVCENAY  +N+A  EGI+ FF++DYRSM+N E+IAPRA+ASC++
Sbjct: 87  KLLAFVSDPALKNNLIVCENAYDIMNKAFLEGIKYFFQRDYRSMVNAERIAPRAEASCSV 146

Query: 212 IFSTPPEKQGLLEERNREIRILIAMAIVS 241
           IF TPPE+   L ERNRE+RILIAMA+VS
Sbjct: 147 IFGTPPERSSPLVERNREMRILIAMALVS 175

[Close]

BLAST of Carg27230 vs. TAIR 10
Match: AT1G09360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 85.9 bits (211), Expect = 5.1e-17
Identity = 54/152 (35.53%), Postives = 78/152 (51.32%), Query Frame = 0


Query: 94  DSDTEDRINNLCHQMEEFGFCSQTFHENLNGPTDDI-GLTQIAIFQAQANASKTLRYIQE 153
           D  TE  IN++C + E++GFC++  HE L  PT  I  LT + I+   A+AS T  +I  
Sbjct: 19  DGPTEALINSICIENEDYGFCNKIIHEKLKTPTATIKELTSLIIYTTMAHASDTYIFIDN 78

Query: 154 LLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCNMI 213
           +L     P   +GL  C   YK    +  E   LF K +Y  M        +    C   
Sbjct: 79  ILRKWPGPKETSGLKTCHAVYKRETNSFLEIRFLFSKGEYELMDEAILSTAKILEECRGD 138

Query: 214 FSTPPEKQGLLEERNREIRILIAMAIVSGYIM 245
           F  PP K+ LLE++ R +RILI M+ VSG+++
Sbjct: 139 FLIPPYKEPLLEKK-RVMRILITMSAVSGHMV 169

[Close]

BLAST of Carg27230 vs. TAIR 10
Match: AT1G09370.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 69.3 bits (168), Expect = 4.9e-12
Identity = 42/154 (27.27%), Postives = 75/154 (48.70%), Query Frame = 0


Query: 94  DSDTEDRINNLCHQMEEFGFCSQTFHENLNG-PTDDIGLTQIAIFQAQANASKTLRYIQE 153
           D  T+ R+N +C Q  +  FCS    +NLN  P  +  +  + + +A+  A+ T  +I  
Sbjct: 23  DEKTQQRVNGICKQTMDTRFCSSLLIKNLNTFPASNKEIMNVTVSEAERFAANTYFFIST 82

Query: 154 LLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCNMI 213
           LL    D   +  L  C  AY  VN A  + +  F +  Y  ++N+EK    A   C   
Sbjct: 83  LLRNAGDE--RPDLQACAEAYAIVNSAFTKAVTFFKQAYYSKIVNIEKKVSMAVDICKTD 142

Query: 214 FSTPPEKQGLLEERNREIRILIAMAIVSGYIMTT 247
           F+    +   L E+NR+ +IL++M  +  +++++
Sbjct: 143 FNVLGYQINPLIEKNRQTKILLSMEQIVSHMVSS 174

[Close]

BLAST of Carg27230 vs. TAIR 10
Match: AT1G56620.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 42.7 bits (99), Expect = 4.9e-04
Identity = 32/149 (21.48%), Postives = 65/149 (43.62%), Query Frame = 0


Query: 94  DSDTEDRINNLCHQMEEFGFCSQTFHENLNGPTDDI-GLTQIAIFQAQANASKTLRYIQE 153
           D+ T   I+++C   +   +C Q+    L  P  +I  +  IA F A    +    YI++
Sbjct: 31  DAPTMILIDSICVNTKNMYYCEQSIISKLGNPHAEIKTIADIAAFNAVYLITSAGNYIRD 90

Query: 154 -LLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCNM 213
             +P   +P +KN L++C   Y+ V + L+       + DY  M + +        +C  
Sbjct: 91  KFIPIAENPLVKNQLVLCLATYRAVQKLLEGAYLADRRGDYSDMRHYQSSVLGMLDNCKT 150

Query: 214 IFSTPPEKQGLLEERNREIRILIAMAIVS 241
            F         +   N  +R++I +++++
Sbjct: 151 DFD-------YMVRTNWGVRLMINISLLA 172

The following BLAST results are available for this feature:

BLAST of Carg27230 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029966.1	2.3e-136	100.00	hypothetical protein SDJN02_08310, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6599000.1	3.2e-82	98.13	hypothetical protein SDJN03_08778, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022946902.1	1.2e-81	97.50	uncharacterized protein LOC111450843 [Cucurbita moschata]	[more]
XP_022972818.1	1.8e-77	92.50	uncharacterized protein LOC111471342 [Cucurbita maxima]	[more]
XP_023546425.1	4.0e-64	80.65	uncharacterized protein LOC111805547 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg27230 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg27230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G4X2	5.9e-82	97.50	uncharacterized protein LOC111450843 OS=Cucurbita moschata OX=3662 GN=LOC1114508...	[more]
A0A6J1IB75	8.9e-78	92.50	uncharacterized protein LOC111471342 OS=Cucurbita maxima OX=3661 GN=LOC111471342...	[more]
A0A6J1KFT2	2.5e-64	81.29	uncharacterized protein LOC111493503 OS=Cucurbita maxima OX=3661 GN=LOC111493503...	[more]
A0A6J1G4V3	1.8e-62	78.71	uncharacterized protein LOC111450827 OS=Cucurbita moschata OX=3662 GN=LOC1114508...	[more]
A0A6J1DRX6	9.3e-51	67.11	uncharacterized protein LOC111023283 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]

BLAST of Carg27230 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G09360.1	5.1e-17	35.53	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G09370.1	4.9e-12	27.27	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G56620.1	4.9e-04	21.48	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 96..240 e-value: 6.5E-10 score: 48.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 101..237 e-value: 2.8E-9 score: 37.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 95..239 e-value: 2.2E-19 score: 67.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 96..241 e-value: 3.1E-17 score: 64.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 98..238
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 65..92
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 74..92
None	No IPR available	PANTHER	PTHR36710:SF1	F14J9.2 PROTEIN	coord: 90..244
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 9..73
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 90..244
None	No IPR available	PANTHER	PTHR36710:SF1	F14J9.2 PROTEIN	coord: 9..73
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 97..241 e-value: 4.31448E-36 score: 123.301

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27230-RA	Carg27230-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity