Carg26027 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg26027
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description1-aminocyclopropane-1-carboxylate synthase 1
LocationCarg_Chr13: 267492 .. 269701 (-)
RNA-Seq ExpressionCarg26027
SyntenyCarg26027
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGTTTCATCAAATCGACGAAAGGAACCAAGCTCTTCTCTCTAAGATCGCCGTAGACGATGGCCATGGCGAGAACTCCCCGTATTTCGATGGATGGAAAGCTTACGATAACGATCCGTTTCACCCCGAGAATAATCCTTTGGGTGTTATTCAAATGGGTTTAGCAGAAAATCAGGTTTTGTATATTGTGTTTTTCATGTTTTTTTTTATGTCATTTCACGTTTGTAAATTTCGCTAACTTTGTTTTTGTGTGAATTTCGATAGCTTTCCTTTGGTATGATTGTTGACTGGCTTAGAAAACACCCCGAAGCTTCCATTTGTACACCGGAAGGACTTGAGAGATTCAAAAGCATTGCCAACTTTCAAGATTACCACGGCTTACCAGAGTTTCGAAATGTACGAGATAATGATATATTCTAACTATATCTAAACTCAAATTTAAGTTGATGGGTTAGGATAAAATTTCTATCTTTTCAGGCGATTGCGAGTTTTATGGGGAAGATAAGAGGTGGGAGGGTGAAATTCGACCCGAGTCGGATTGTGATGGGTGGCGGTGCGACCGGCGCGAGCGAAACCGTCATCTTTTGTCTGGCGGATCCGGGGGATGCTTTTTTAGTTCCTTCTCCATATTATGCAGCGTGAGTTCTTCTTTCATTTCCTTTTGTTCACTTTTCTTAAGTCAATATTCCTTAGTCGAACCTGGAAAGAGAAAGAAGAGAGAGAAAGAAACCATTTGACAAATTAATATAACTCTACAAATTCTCTTTGAAAGTATGATGCTTTTTTTAGGGTCAAAGCTTCAACCATTCTCTCCCAAAAAAAAAAAAATGTCATAATTATAATGAAGAAAAAACTAGGCCATCCAAGTCAACCTTTTTAAATGCTAACAAAGTCAATATGCTTTATAGGTTAAAAAAACAATAAATTGCTTAATCATTTCTTAAACTTTAATTAAACCCTTTTGACTTTCATCATTACCCATTTACATCATTTAACAATTTAGTACTCTTTTTGTAGTAAAATTAATAGAAAAGTTACGTGTAAACGAATAGTATCATGTTATTGTAAAAATACTGAGAAGGGCTGGTATGTTGTTTTTCAGGTTTGATCGAGACATCAAATGGCGAACACGAGCACAAATAATTCGGGTCCATTGCAACAGCTCGAACAACTTCCAAGTCACAAAGGCAGCCTTAGAAATAGCCTACAAAAAGGCTCAAGAGGCCAACATCAAAGTGAAGGGTGTCATAATCACCAATCCCTCAAATCCCTTAGGCACAACATACGACCGTGACACTCTCAAAACCCTCGTCACCTTTGTGAATCAACACGACATTCACTTAATCTGCGATGAAATATACTCTGCCACTGTCTTCAAAGCCCCAACCTTCACCAGCATCGCTGAGATTGTTGAACAAATGGAGCATTGCAAGAAGGAGCTCATCCATATTCTTTATAGCTTGTCCAAAGACATGGGCCTCCCTGGTTTTCGAGTCGGAATTATTTATTCTTACAACGATCTCGTCGTCCGCCGTGCTCGGCAGATCGTCGAGCTTTGGTCTAGTCTCGTCCCCAGACTCAACATTTGCTCGCCGCCATTTGCTTTCCCGACGAGAGGACTTTGTCGACAAATTTCTTGCCGAGAACTCGAAGCGCCTCGCGGAGAGGCATGCAAGGGTTTGTTAAACTAAACCATTATTATTGTAGGATTGAAGAGCATTACAAAATGCAATTAAATTTAAAATGTATTAATCAAATTCAGGTTCACAAAAGAATTGGATAAAATGGGGATCACTTGCTTGAACAGCAATGCTGGAGTTTTTGTGTGGATGGATCTACGGAGGCTATCTAAAAGACCAAACCTTCAAAGCTGAAATGGAGCTTTGGCGTGTGATTATCAATGAAGTCAAGCTCAATGTTTCTCCTGGCTCATCCTTTCATGTCAACGAGCCAGGTTGGTTTCGAGTTTGTTTCGCAAACATGGACGACAACACCGTCGACGTTGCTCTCAATAGAATCCATAGCTTTGTCAAAAACATCGACAAGAAGAAAGACAATACCGTTGCAATGCCATCGAAAACGAGGCGTCGAGAGAATAAGTTACGATTGAGCTTCTCCTTCTCAGGGAGAAGATATGACGAGGGCAACGTTCTTAACTCACCGCACACGATGTCGCCTCACTCGCCATTAGTAATAGCAAAAAATTAA

mRNA sequence

ATGGGGTTTCATCAAATCGACGAAAGGAACCAAGCTCTTCTCTCTAAGATCGCCGTAGACGATGGCCATGGCGAGAACTCCCCGTATTTCGATGGATGGAAAGCTTACGATAACGATCCGTTTCACCCCGAGAATAATCCTTTGGGTGTTATTCAAATGGGTTTAGCAGAAAATCAGCTTTCCTTTGGTATGATTGTTGACTGGCTTAGAAAACACCCCGAAGCTTCCATTTGTACACCGGAAGGACTTGAGAGATTCAAAAGCATTGCCAACTTTCAAGATTACCACGGCTTACCAGAGTTTCGAAATGCGATTGCGAGTTTTATGGGGAAGATAAGAGGTGGGAGGGTGAAATTCGACCCGAGTCGGATTGTGATGGGTGGCGGTGCGACCGGCGCGAGCGAAACCGTCATCTTTTGTCTGGCGGATCCGGGGGATGCTTTTTTAGTTCCTTCTCCATATTATGCAGCGTTTGATCGAGACATCAAATGGCGAACACGAGCACAAATAATTCGGGTCCATTGCAACAGCTCGAACAACTTCCAAGTCACAAAGGCAGCCTTAGAAATAGCCTACAAAAAGGCTCAAGAGGCCAACATCAAAGTGAAGGGTGTCATAATCACCAATCCCTCAAATCCCTTAGGCACAACATACGACCGTGACACTCTCAAAACCCTCGTCACCTTTGTGAATCAACACGACATTCACTTAATCTGCGATGAAATATACTCTGCCACTGTCTTCAAAGCCCCAACCTTCACCAGCATCGCTGAGATTGTTGAACAAATGGAGCATTGCAAGAAGGAGCTCATCCATATTCTTTATAGCTTGTCCAAAGACATGGGCCTCCCTGGTTTTCGAGTCGGAATTATTTATTCTTACAACGATCTCGTCGTCCGCCGTGCTCGGCAGATCGTCGAGCTTTGGTCTAGTCTCGTCCCCAGACTCAACATTTGCTCGCCGCCATTTGCTTTCCCGACGAGAGGACTTTGTCGACAAATTTCTTGCCGAGAACTCGAAGCGCCTCGCGGAGAGGCATGCAAGGCAATGCTGGAGTTTTTGTGTGGATGGATCTACGGAGGCTATCTAAAAGACCAAACCTTCAAAGCTGAAATGGAGCTTTGGCGTGTGATTATCAATGAAGTCAAGCTCAATGTTTCTCCTGGCTCATCCTTTCATGTCAACGAGCCAGGTTGGTTTCGAGTTTGTTTCGCAAACATGGACGACAACACCGTCGACGTTGCTCTCAATAGAATCCATAGCTTTGTCAAAAACATCGACAAGAAGAAAGACAATACCGTTGCAATGCCATCGAAAACGAGGCGTCGAGAGAATAAGTTACGATTGAGCTTCTCCTTCTCAGGGAGAAGATATGACGAGGGCAACGTTCTTAACTCACCGCACACGATGTCGCCTCACTCGCCATTAGTAATAGCAAAAAATTAA

Coding sequence (CDS)

ATGGGGTTTCATCAAATCGACGAAAGGAACCAAGCTCTTCTCTCTAAGATCGCCGTAGACGATGGCCATGGCGAGAACTCCCCGTATTTCGATGGATGGAAAGCTTACGATAACGATCCGTTTCACCCCGAGAATAATCCTTTGGGTGTTATTCAAATGGGTTTAGCAGAAAATCAGCTTTCCTTTGGTATGATTGTTGACTGGCTTAGAAAACACCCCGAAGCTTCCATTTGTACACCGGAAGGACTTGAGAGATTCAAAAGCATTGCCAACTTTCAAGATTACCACGGCTTACCAGAGTTTCGAAATGCGATTGCGAGTTTTATGGGGAAGATAAGAGGTGGGAGGGTGAAATTCGACCCGAGTCGGATTGTGATGGGTGGCGGTGCGACCGGCGCGAGCGAAACCGTCATCTTTTGTCTGGCGGATCCGGGGGATGCTTTTTTAGTTCCTTCTCCATATTATGCAGCGTTTGATCGAGACATCAAATGGCGAACACGAGCACAAATAATTCGGGTCCATTGCAACAGCTCGAACAACTTCCAAGTCACAAAGGCAGCCTTAGAAATAGCCTACAAAAAGGCTCAAGAGGCCAACATCAAAGTGAAGGGTGTCATAATCACCAATCCCTCAAATCCCTTAGGCACAACATACGACCGTGACACTCTCAAAACCCTCGTCACCTTTGTGAATCAACACGACATTCACTTAATCTGCGATGAAATATACTCTGCCACTGTCTTCAAAGCCCCAACCTTCACCAGCATCGCTGAGATTGTTGAACAAATGGAGCATTGCAAGAAGGAGCTCATCCATATTCTTTATAGCTTGTCCAAAGACATGGGCCTCCCTGGTTTTCGAGTCGGAATTATTTATTCTTACAACGATCTCGTCGTCCGCCGTGCTCGGCAGATCGTCGAGCTTTGGTCTAGTCTCGTCCCCAGACTCAACATTTGCTCGCCGCCATTTGCTTTCCCGACGAGAGGACTTTGTCGACAAATTTCTTGCCGAGAACTCGAAGCGCCTCGCGGAGAGGCATGCAAGGCAATGCTGGAGTTTTTGTGTGGATGGATCTACGGAGGCTATCTAAAAGACCAAACCTTCAAAGCTGAAATGGAGCTTTGGCGTGTGATTATCAATGAAGTCAAGCTCAATGTTTCTCCTGGCTCATCCTTTCATGTCAACGAGCCAGGTTGGTTTCGAGTTTGTTTCGCAAACATGGACGACAACACCGTCGACGTTGCTCTCAATAGAATCCATAGCTTTGTCAAAAACATCGACAAGAAGAAAGACAATACCGTTGCAATGCCATCGAAAACGAGGCGTCGAGAGAATAAGTTACGATTGAGCTTCTCCTTCTCAGGGAGAAGATATGACGAGGGCAACGTTCTTAACTCACCGCACACGATGTCGCCTCACTCGCCATTAGTAATAGCAAAAAATTAA

Protein sequence

MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQLSFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFDPSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNNFQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICDEIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVRRARQIVELWSSLVPRLNICSPPFAFPTRGLCRQISCRELEAPRGEACKAMLEFLCGWIYGGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANMDDNTVDVALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSPHTMSPHSPLVIAKN
Homology
BLAST of Carg26027 vs. NCBI nr
Match: KAG7019125.1 (1-aminocyclopropane-1-carboxylate synthase CMW33, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 998.0 bits (2579), Expect = 2.7e-287
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0

Query: 1   MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60
           MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL
Sbjct: 1   MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60

Query: 61  SFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFD 120
           SFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFD
Sbjct: 61  SFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFD 120

Query: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNN 180
           PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNN
Sbjct: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNN 180

Query: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240
           FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD
Sbjct: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240

Query: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR 300
           EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR
Sbjct: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR 300

Query: 301 RARQIVELWSSLVPRLNICSPPFAFPTRGLCRQISCRELEAPRGEACKAMLEFLCGWIYG 360
           RARQIVELWSSLVPRLNICSPPFAFPTRGLCRQISCRELEAPRGEACKAMLEFLCGWIYG
Sbjct: 301 RARQIVELWSSLVPRLNICSPPFAFPTRGLCRQISCRELEAPRGEACKAMLEFLCGWIYG 360

Query: 361 GYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANMDDNTVDVALNRIH 420
           GYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANMDDNTVDVALNRIH
Sbjct: 361 GYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANMDDNTVDVALNRIH 420

Query: 421 SFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSPHTMSPHSPLVIAK 480
           SFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSPHTMSPHSPLVIAK
Sbjct: 421 SFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSPHTMSPHSPLVIAK 480

Query: 481 N 482
           N
Sbjct: 481 N 481

BLAST of Carg26027 vs. NCBI nr
Match: KAG6575198.1 (1-aminocyclopropane-1-carboxylate synthase CMW33, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 837.0 bits (2161), Expect = 8.0e-239
Identity = 420/494 (85.02%), Postives = 440/494 (89.07%), Query Frame = 0

Query: 1   MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60
           MG HQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL
Sbjct: 1   MGLHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60

Query: 61  SFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFD 120
           SF MIVDW+RKHPEASICTPEGLERFKSIANFQDYHGL EFRN IASFMGK+RGGRVKF+
Sbjct: 61  SFDMIVDWIRKHPEASICTPEGLERFKSIANFQDYHGLSEFRNGIASFMGKVRGGRVKFN 120

Query: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNN 180
           PSRIVMGGGATGASET+IFCLADPGDAFLVPSPYYA FDRD+KWRTRAQIIRVHCNSSNN
Sbjct: 121 PSRIVMGGGATGASETLIFCLADPGDAFLVPSPYYAGFDRDLKWRTRAQIIRVHCNSSNN 180

Query: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240
           FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD
Sbjct: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240

Query: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR 300
           EIYSATVF+APTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR
Sbjct: 241 EIYSATVFEAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR 300

Query: 301 RARQI----------VELWSSLVPRLNICSPPFAFPTRGLCRQIS--CRELEAPRGEACK 360
           RARQ+            L ++++   +      A  ++ L  + +   +EL+   G  C 
Sbjct: 301 RARQMSSFGLVSSQTQHLLAAMLSDEDFVDKFLAENSKRLAERHARFTKELD-KMGITCL 360

Query: 361 AMLEFLCGWI-YGGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANM 420
                +  W+     LKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANM
Sbjct: 361 NSNAGVFVWMDLRRLLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANM 420

Query: 421 DDNTVDVALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP 480
           DDNTVDVALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP
Sbjct: 421 DDNTVDVALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP 480

Query: 481 HTMSPHSPLVIAKN 482
           HTMSPHSPLVIAKN
Sbjct: 481 HTMSPHSPLVIAKN 493

BLAST of Carg26027 vs. NCBI nr
Match: XP_023547768.1 (1-aminocyclopropane-1-carboxylate synthase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 832.4 bits (2149), Expect = 2.0e-237
Identity = 416/494 (84.21%), Postives = 440/494 (89.07%), Query Frame = 0

Query: 1   MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60
           MGFHQIDERNQALLSKIA+DDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL
Sbjct: 1   MGFHQIDERNQALLSKIALDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60

Query: 61  SFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFD 120
           SF MIVDW+RKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIA+FMGK+RGGRVKFD
Sbjct: 61  SFDMIVDWIRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIANFMGKVRGGRVKFD 120

Query: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNN 180
           PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYA FDRD+KWRTRAQIIRVHCN SNN
Sbjct: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAGFDRDLKWRTRAQIIRVHCNRSNN 180

Query: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240
           FQVTKAALEIAYKKAQEAN+KVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD
Sbjct: 181 FQVTKAALEIAYKKAQEANMKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240

Query: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR 300
           EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYND+VVR
Sbjct: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDVVVR 300

Query: 301 RARQI----------VELWSSLVPRLNICSPPFAFPTRGLCRQIS--CRELEAPRGEACK 360
           RARQ+            L ++++   +      A  ++ L  + +   +EL+   G  C 
Sbjct: 301 RARQMSSFGLVSSQTQHLLAAMLSDEDFVDKFLAENSKRLGERHARFTKELD-KMGITCL 360

Query: 361 AMLEFLCGWI-YGGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANM 420
                +  W+     LKDQTFKAEMELWRVIINEVKLNVSPGSSFHV EPGWFRVCFANM
Sbjct: 361 NSNAGVFVWMDLRRLLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVTEPGWFRVCFANM 420

Query: 421 DDNTVDVALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP 480
           DDNTVDVALNRIHSFV+NIDKK+DNTVAMPSKTR R+NKLRLSFSFSGRRYDEGNVLNSP
Sbjct: 421 DDNTVDVALNRIHSFVENIDKKEDNTVAMPSKTRHRDNKLRLSFSFSGRRYDEGNVLNSP 480

Query: 481 HTMSPHSPLVIAKN 482
           HTMSPHSPLVIAKN
Sbjct: 481 HTMSPHSPLVIAKN 493

BLAST of Carg26027 vs. NCBI nr
Match: P23279.1 (RecName: Full=1-aminocyclopropane-1-carboxylate synthase 1; Short=ACC synthase 1; AltName: Full=S-adenosyl-L-methionine methylthioadenosine-lyase [Cucurbita pepo] >AAA33113.1 1-aminocyclopropane-1-carboxylate synthase [Cucurbita pepo])

HSP 1 Score: 831.6 bits (2147), Expect = 3.4e-237
Identity = 415/494 (84.01%), Postives = 440/494 (89.07%), Query Frame = 0

Query: 1   MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60
           MGFHQIDERNQALLSKIA+DDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL
Sbjct: 1   MGFHQIDERNQALLSKIALDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60

Query: 61  SFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFD 120
           SF MIVDW+RKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIA+FMGK+RGGRVKFD
Sbjct: 61  SFDMIVDWIRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIANFMGKVRGGRVKFD 120

Query: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNN 180
           PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYA FDRD+KWRTRAQIIRVHCN SNN
Sbjct: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAGFDRDLKWRTRAQIIRVHCNGSNN 180

Query: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240
           FQVTKAALEIAYKKAQEAN+KVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD
Sbjct: 181 FQVTKAALEIAYKKAQEANMKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240

Query: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR 300
           EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYND+VVR
Sbjct: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDVVVR 300

Query: 301 RARQI----------VELWSSLVPRLNICSPPFAFPTRGLCRQIS--CRELEAPRGEACK 360
           RARQ+            L ++++   +      A  ++ +  + +   +EL+   G  C 
Sbjct: 301 RARQMSSFGLVSSQTQHLLAAMLSDEDFVDKFLAENSKRVGERHARFTKELD-KMGITCL 360

Query: 361 AMLEFLCGWI-YGGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANM 420
                +  W+     LKDQTFKAEMELWRVIINEVKLNVSPGSSFHV EPGWFRVCFANM
Sbjct: 361 NSNAGVFVWMDLRRLLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVTEPGWFRVCFANM 420

Query: 421 DDNTVDVALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP 480
           DDNTVDVALNRIHSFV+NIDKK+DNTVAMPSKTR R+NKLRLSFSFSGRRYDEGNVLNSP
Sbjct: 421 DDNTVDVALNRIHSFVENIDKKEDNTVAMPSKTRHRDNKLRLSFSFSGRRYDEGNVLNSP 480

Query: 481 HTMSPHSPLVIAKN 482
           HTMSPHSPLVIAKN
Sbjct: 481 HTMSPHSPLVIAKN 493

BLAST of Carg26027 vs. NCBI nr
Match: AAA33111.1 (1-aminocyclopropane-1-carboxylate synthase [Cucurbita pepo])

HSP 1 Score: 831.2 bits (2146), Expect = 4.4e-237
Identity = 415/494 (84.01%), Postives = 440/494 (89.07%), Query Frame = 0

Query: 1   MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60
           MGFHQIDERNQALLSKIA+DDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL
Sbjct: 1   MGFHQIDERNQALLSKIALDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60

Query: 61  SFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFD 120
           SF MIVDW+RKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIA+FMGK+RGGRVKFD
Sbjct: 61  SFDMIVDWIRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIANFMGKVRGGRVKFD 120

Query: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNN 180
           PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYA FDRD+KWRTRAQIIRVHCN SNN
Sbjct: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAGFDRDLKWRTRAQIIRVHCNRSNN 180

Query: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240
           FQVTKAALEIAYKKAQEAN+KVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD
Sbjct: 181 FQVTKAALEIAYKKAQEANMKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240

Query: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR 300
           EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYND+VVR
Sbjct: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDVVVR 300

Query: 301 RARQI----------VELWSSLVPRLNICSPPFAFPTRGLCRQIS--CRELEAPRGEACK 360
           RARQ+            L ++++   +      A  ++ +  + +   +EL+   G  C 
Sbjct: 301 RARQMSSFGLVSSQTQHLLAAMLSDEDFVDKFLAENSKRVGERHARFTKELD-KMGITCL 360

Query: 361 AMLEFLCGWI-YGGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANM 420
                +  W+     LKDQTFKAEMELWRVIINEVKLNVSPGSSFHV EPGWFRVCFANM
Sbjct: 361 NSNAGVFVWMDLRRLLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVTEPGWFRVCFANM 420

Query: 421 DDNTVDVALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP 480
           DDNTVDVALNRIHSFV+NIDKK+DNTVAMPSKTR R+NKLRLSFSFSGRRYDEGNVLNSP
Sbjct: 421 DDNTVDVALNRIHSFVENIDKKEDNTVAMPSKTRHRDNKLRLSFSFSGRRYDEGNVLNSP 480

Query: 481 HTMSPHSPLVIAKN 482
           HTMSPHSPLVIAKN
Sbjct: 481 HTMSPHSPLVIAKN 493

BLAST of Carg26027 vs. ExPASy Swiss-Prot
Match: P23279 (1-aminocyclopropane-1-carboxylate synthase 1 OS=Cucurbita pepo OX=3663 GN=ACC1A PE=1 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 4.4e-240
Identity = 415/494 (84.01%), Postives = 440/494 (89.07%), Query Frame = 0

Query: 1   MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60
           MGFHQIDERNQALLSKIA+DDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL
Sbjct: 1   MGFHQIDERNQALLSKIALDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60

Query: 61  SFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFD 120
           SF MIVDW+RKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIA+FMGK+RGGRVKFD
Sbjct: 61  SFDMIVDWIRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIANFMGKVRGGRVKFD 120

Query: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNN 180
           PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYA FDRD+KWRTRAQIIRVHCN SNN
Sbjct: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAGFDRDLKWRTRAQIIRVHCNGSNN 180

Query: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240
           FQVTKAALEIAYKKAQEAN+KVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD
Sbjct: 181 FQVTKAALEIAYKKAQEANMKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240

Query: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR 300
           EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYND+VVR
Sbjct: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDVVVR 300

Query: 301 RARQI----------VELWSSLVPRLNICSPPFAFPTRGLCRQIS--CRELEAPRGEACK 360
           RARQ+            L ++++   +      A  ++ +  + +   +EL+   G  C 
Sbjct: 301 RARQMSSFGLVSSQTQHLLAAMLSDEDFVDKFLAENSKRVGERHARFTKELD-KMGITCL 360

Query: 361 AMLEFLCGWI-YGGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANM 420
                +  W+     LKDQTFKAEMELWRVIINEVKLNVSPGSSFHV EPGWFRVCFANM
Sbjct: 361 NSNAGVFVWMDLRRLLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVTEPGWFRVCFANM 420

Query: 421 DDNTVDVALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP 480
           DDNTVDVALNRIHSFV+NIDKK+DNTVAMPSKTR R+NKLRLSFSFSGRRYDEGNVLNSP
Sbjct: 421 DDNTVDVALNRIHSFVENIDKKEDNTVAMPSKTRHRDNKLRLSFSFSGRRYDEGNVLNSP 480

Query: 481 HTMSPHSPLVIAKN 482
           HTMSPHSPLVIAKN
Sbjct: 481 HTMSPHSPLVIAKN 493

BLAST of Carg26027 vs. ExPASy Swiss-Prot
Match: P23599 (1-aminocyclopropane-1-carboxylate synthase CMW33 OS=Cucurbita maxima OX=3661 GN=ACS1 PE=2 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 2.2e-239
Identity = 415/494 (84.01%), Postives = 440/494 (89.07%), Query Frame = 0

Query: 1   MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60
           M FHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPE+NPLGVIQMGLAENQL
Sbjct: 1   MEFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPEDNPLGVIQMGLAENQL 60

Query: 61  SFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFD 120
           SF MIVDW+RKHPEASICTP+GLERFKSIANFQDYHGLPEFRN IASFMGK+RGGRV+FD
Sbjct: 61  SFDMIVDWIRKHPEASICTPKGLERFKSIANFQDYHGLPEFRNGIASFMGKVRGGRVQFD 120

Query: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNN 180
           PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRD+KWRTRAQIIRVHCNSSNN
Sbjct: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDLKWRTRAQIIRVHCNSSNN 180

Query: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240
           FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD
Sbjct: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240

Query: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR 300
           EIYSATVFKAPTF SIA+IVE+MEHCKKELIHILYSLSKDMGLPGFRVGIIYSYND+VVR
Sbjct: 241 EIYSATVFKAPTFISIAQIVEEMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDVVVR 300

Query: 301 RARQI----------VELWSSLVPRLNICSPPFAFPTRGLCRQIS--CRELEAPRGEACK 360
           RARQ+            L ++++   +      A  ++ L  + +   +EL+   G  C 
Sbjct: 301 RARQMSSFGLVSSQTQHLLAAMLSDEDFVDKFLAENSKRLAERHARFTKELD-KMGITCL 360

Query: 361 AMLEFLCGWI-YGGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANM 420
                +  W+     LKDQTFKAEMELWRVIINEVKLNVSPGSSFHV EPGWFRVCFANM
Sbjct: 361 NSNAGVFVWMDLRRLLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVTEPGWFRVCFANM 420

Query: 421 DDNTVDVALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP 480
           DDNTVDVALNRIHSFV+NIDKK+DNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP
Sbjct: 421 DDNTVDVALNRIHSFVENIDKKEDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP 480

Query: 481 HTMSPHSPLVIAKN 482
           HTMSPHSPLVIAKN
Sbjct: 481 HTMSPHSPLVIAKN 493

BLAST of Carg26027 vs. ExPASy Swiss-Prot
Match: Q00379 (1-aminocyclopropane-1-carboxylate synthase 2 OS=Cucurbita pepo OX=3663 GN=ACS2 PE=2 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 2.5e-235
Identity = 408/493 (82.76%), Postives = 437/493 (88.64%), Query Frame = 0

Query: 1   MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60
           MGFHQIDERNQALLSKIA+DDGHGENS YFDGWKAYDN+PFHPENNPLGVIQMGLAENQL
Sbjct: 1   MGFHQIDERNQALLSKIAIDDGHGENSAYFDGWKAYDNNPFHPENNPLGVIQMGLAENQL 60

Query: 61  SFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFD 120
           SFGMIVDW+RKHPEASICTPEGLE+FKSIANFQDYHGL EFR A+ASFMGK+RGGRVKFD
Sbjct: 61  SFGMIVDWIRKHPEASICTPEGLEKFKSIANFQDYHGLQEFRKAMASFMGKVRGGRVKFD 120

Query: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNN 180
           PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRD+KWRTRAQII VHCNSSNN
Sbjct: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDLKWRTRAQIIPVHCNSSNN 180

Query: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240
           FQVT+AALEIAYKKAQEAN+KVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD
Sbjct: 181 FQVTEAALEIAYKKAQEANMKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240

Query: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR 300
           EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYND+VVR
Sbjct: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDVVVR 300

Query: 301 RARQI----------VELWSSLVPRLNICSPPFAFPTRGLCRQIS--CRELEAPRGEACK 360
           RARQ+            L ++++   +      A  ++ L  + +   +EL+   G  C 
Sbjct: 301 RARQMSSFGLVSSQTQHLLAAMLSDEDFVDKFLAENSKRLGERHARFTKELD-KMGITCL 360

Query: 361 AMLEFLCGWI-YGGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANM 420
                +  W+     LKDQTFKAEMELWRVIINEVKLNVSPGSSFHV EPGWFRVCFANM
Sbjct: 361 NSNAGVFVWMDLRRLLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVTEPGWFRVCFANM 420

Query: 421 DDNTVDVALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP 480
           DDNTVDVALNRIHSFV+NIDKK+DNTVAMPSKTR R+NKLRLSFSFSGRRYD+GNVLNSP
Sbjct: 421 DDNTVDVALNRIHSFVENIDKKEDNTVAMPSKTRHRDNKLRLSFSFSGRRYDKGNVLNSP 480

BLAST of Carg26027 vs. ExPASy Swiss-Prot
Match: Q9MB95 (1-aminocyclopropane-1-carboxylate synthase 1 OS=Prunus mume OX=102107 GN=ACS1 PE=2 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 3.7e-162
Identity = 295/489 (60.33%), Postives = 352/489 (71.98%), Query Frame = 0

Query: 10  NQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQLSFGMIVDWL 69
           N+ LLSKIA  +GHGENSPYFDGWKAYD +PFHP  NP GVIQMGLAENQLSF  I DW+
Sbjct: 9   NRFLLSKIATSEGHGENSPYFDGWKAYDRNPFHPTKNPEGVIQMGLAENQLSFDSIEDWI 68

Query: 70  RKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFDPSRIVMGGG 129
           +K+P+ASICTPEG+E FK++A FQDYHG PEFR A+A FM K RGGRV FDP+R+VM GG
Sbjct: 69  KKNPKASICTPEGVEEFKNVAIFQDYHGFPEFRKAVAMFMSKARGGRVTFDPNRVVMSGG 128

Query: 130 ATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNNFQVTKAALE 189
           ATGA+E V+FCLADPGDAFLVPSPYY AF RD+ WRT  QI+ V C+SSNNF++TK ALE
Sbjct: 129 ATGANELVMFCLADPGDAFLVPSPYYPAFFRDLGWRTGVQIVPVDCDSSNNFKITKEALE 188

Query: 190 IAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICDEIYSATVFK 249
            AY+KAQ+ NI VKG+IITNPSNPLGTT DR+TL++LV F+NQ +IHL+CDEIY+ATVF 
Sbjct: 189 AAYEKAQKNNINVKGLIITNPSNPLGTTLDRNTLESLVEFINQKNIHLVCDEIYAATVFS 248

Query: 250 APTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVRRARQI---- 309
           +PTFT I+E+++ M +C   LIHI+YSLSKDMGLPG RVGI+YSYND VV   R++    
Sbjct: 249 SPTFTCISEVIQNM-NCNPNLIHIVYSLSKDMGLPGLRVGIVYSYNDDVVNIGRKMSSFG 308

Query: 310 ------------VELWSSLVPRLNICSPPFAFPTRGLCRQISCRELEAPRGEACKAMLEF 369
                       + L    V R    SP       G    +  + LE       K+    
Sbjct: 309 LVSSQTQHMLPSMLLDEEFVARFLETSPKRLAKRHG----VFTKGLEEVGINCLKSNAGL 368

Query: 370 LCGWIYGGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANMDDNTVD 429
            C       L+DQTF  EM LWRVI+NEV  NVSPGSSF   EPGWFRVCFANMDD T++
Sbjct: 369 FCWMDLRRLLEDQTFDGEMVLWRVIVNEVGPNVSPGSSFKCVEPGWFRVCFANMDDETLE 428

Query: 430 VALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSG-RRYDEGNV-LNSPHTMS 481
           VAL RI +FV+   K +D  V + S  R + N LRLSFS S  RR+D+ +V + SPH MS
Sbjct: 429 VALKRIRTFVRQGKKAQDQVVQVKSPKRWKSN-LRLSFSSSSTRRFDQESVNVLSPHMMS 488

BLAST of Carg26027 vs. ExPASy Swiss-Prot
Match: Q07262 (1-aminocyclopropane-1-carboxylate synthase OS=Nicotiana tabacum OX=4097 GN=ACS1 PE=2 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 1.7e-159
Identity = 288/495 (58.18%), Postives = 358/495 (72.32%), Query Frame = 0

Query: 1   MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60
           MGF   +E+N ++LSK+A ++  GENSPYFDGWKAYDNDPFHP  NP GVIQMGLAENQL
Sbjct: 1   MGFE--NEKNSSILSKLATNEELGENSPYFDGWKAYDNDPFHPLKNPNGVIQMGLAENQL 60

Query: 61  SFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFD 120
            F +I +W++++P ASICT EG++ F++IANFQDYHGLPEFR+AIA FM K RGGRV FD
Sbjct: 61  CFDLIEEWIKRNPNASICTTEGIKSFRAIANFQDYHGLPEFRSAIAKFMEKTRGGRVTFD 120

Query: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNN 180
           P R+VM GGATGA+ET+IFCLAD GDAFLVPSPYY AF+RD++WRT  Q+I + C+SSNN
Sbjct: 121 PERVVMAGGATGANETIIFCLADTGDAFLVPSPYYPAFNRDLRWRTGVQLIPIPCDSSNN 180

Query: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240
           FQ+T  A+  AY+ AQ++NIKVKG+I+TNPSNPLGTT DRDTLK L+TF NQH+IHL+CD
Sbjct: 181 FQITTKAVREAYENAQKSNIKVKGLILTNPSNPLGTTLDRDTLKNLLTFTNQHNIHLVCD 240

Query: 241 EIYSATVFKAPTFTSIAEIV-EQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVV 300
           EIY+ATVF  P F SIAEI+ ++  HC K+L+HI+YSLSKDMGLPGFRVGI+YS+ND VV
Sbjct: 241 EIYAATVFNTPQFVSIAEILDDETSHCNKDLVHIVYSLSKDMGLPGFRVGIVYSFNDAVV 300

Query: 301 RRAR----------QIVELWSSLVPRLNICSPPFAFPTRGLCRQIS--CRELEAPRGEAC 360
             AR          Q   L + ++      S      ++ L ++       LE    +  
Sbjct: 301 NCARKMSSFGLVSTQTQYLLAEMLSDERFVSNFLTESSKRLAKRHKHFTNGLEEVGIKCL 360

Query: 361 KAMLEFLCGWIYGGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANM 420
           ++     C       LK+ TF +EM LWRVIIN+VKLNVSPGSSF   EPG+FRVCFANM
Sbjct: 361 RSNAGLFCWMDLRPLLKESTFDSEMSLWRVIINDVKLNVSPGSSFDCQEPGFFRVCFANM 420

Query: 421 DDNTVDVALNRIHSFVKNIDKKKDNT--VAMPSKTRRRENKLRLSFSFSGRRYDEGNVLN 480
           DD TVD+AL RI SFV  + K  D +  + M  K + ++N LRL  SFS R YDE   L+
Sbjct: 421 DDETVDIALARIRSFV-GVKKSGDESTPILMEKKQQWKKNNLRL--SFSKRMYDESVNLS 480

BLAST of Carg26027 vs. ExPASy TrEMBL
Match: A0A6J1KY37 (1-aminocyclopropane-1-carboxylate synthase CMW33 OS=Cucurbita maxima OX=3661 GN=LOC111499253 PE=3 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 8.1e-237
Identity = 415/494 (84.01%), Postives = 440/494 (89.07%), Query Frame = 0

Query: 1   MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60
           M FHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPE+NPLGVIQMGLAENQL
Sbjct: 1   MEFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPEDNPLGVIQMGLAENQL 60

Query: 61  SFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFD 120
           SF MIVDW+RKHPEASICTP+GLERFKSIANFQDYHGLPEFRN IASFMGK+RGGRV+FD
Sbjct: 61  SFDMIVDWIRKHPEASICTPKGLERFKSIANFQDYHGLPEFRNGIASFMGKVRGGRVQFD 120

Query: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNN 180
           PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRD+KWRTRAQIIRVHCNSSNN
Sbjct: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDLKWRTRAQIIRVHCNSSNN 180

Query: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240
           FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD
Sbjct: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240

Query: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR 300
           EIYSATVFKAPTF SIA+IVE+MEHCKKELIHILYSLSKDMGLPGFRVGIIYSYND+VVR
Sbjct: 241 EIYSATVFKAPTFISIAQIVEEMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDVVVR 300

Query: 301 RARQI----------VELWSSLVPRLNICSPPFAFPTRGLCRQIS--CRELEAPRGEACK 360
           RARQ+            L ++++   +      A  ++ L  + +   +EL+   G  C 
Sbjct: 301 RARQMSSFGLVSSQTQHLLAAMLSDEDFVDKFLAENSKRLAERHARFTKELD-KMGITCL 360

Query: 361 AMLEFLCGWI-YGGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANM 420
                +  W+     LKDQTFKAEMELWRVIINEVKLNVSPGSSFHV EPGWFRVCFANM
Sbjct: 361 NSNAGVFVWMDLRRLLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVTEPGWFRVCFANM 420

Query: 421 DDNTVDVALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP 480
           DDNTVDVALNRIHSFV+NIDKK+DNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP
Sbjct: 421 DDNTVDVALNRIHSFVENIDKKEDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP 480

Query: 481 HTMSPHSPLVIAKN 482
           HTMSPHSPLVIAKN
Sbjct: 481 HTMSPHSPLVIAKN 493

BLAST of Carg26027 vs. ExPASy TrEMBL
Match: A0A6J1H427 (1-aminocyclopropane-1-carboxylate synthase 1 OS=Cucurbita moschata OX=3662 GN=LOC111460306 PE=3 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 6.9e-236
Identity = 416/494 (84.21%), Postives = 437/494 (88.46%), Query Frame = 0

Query: 1   MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60
           MGFHQIDERN ALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL
Sbjct: 1   MGFHQIDERNLALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60

Query: 61  SFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFD 120
           SF MIVDW+RKHPEASICT EGLERFKSIANFQDYHGL EFRN IASFMGK+RGGRVKF+
Sbjct: 61  SFDMIVDWIRKHPEASICTLEGLERFKSIANFQDYHGLSEFRNGIASFMGKVRGGRVKFN 120

Query: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNN 180
           PSRIVMGGGATGASET+IFCLADPGDAFLVPSPYYA FDRD+KWRTRAQIIRVHCNSSNN
Sbjct: 121 PSRIVMGGGATGASETLIFCLADPGDAFLVPSPYYAGFDRDLKWRTRAQIIRVHCNSSNN 180

Query: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240
           FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD
Sbjct: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240

Query: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR 300
           EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYN LVVR
Sbjct: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNVLVVR 300

Query: 301 RARQI----------VELWSSLVPRLNICSPPFAFPTRGLCRQIS--CRELEAPRGEACK 360
           RARQ+            L ++++   +      A  ++ L  + +   +EL+   G  C 
Sbjct: 301 RARQMSSFGLVSSQTQHLLAAMLSDEDFVDKFLAENSKRLAERHARFTKELD-KMGITCL 360

Query: 361 AMLEFLCGWI-YGGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANM 420
                +  W+     LKDQTFKAEMELWRVIIN+VKLNVSPGSSF VNEPGWFRVCFANM
Sbjct: 361 NSNAGVFVWMDLRRLLKDQTFKAEMELWRVIINQVKLNVSPGSSFRVNEPGWFRVCFANM 420

Query: 421 DDNTVDVALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP 480
           DDNTVD+ALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP
Sbjct: 421 DDNTVDIALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP 480

Query: 481 HTMSPHSPLVIAKN 482
           HTMSPHSPLVIAKN
Sbjct: 481 HTMSPHSPLVIAKN 493

BLAST of Carg26027 vs. ExPASy TrEMBL
Match: B2WR81 (1-aminocyclopropane-1-carboxylate synthase OS=Citrullus colocynthis x Citrullus lanatus var. lanatus OX=433968 GN=ACS3 PE=3 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 4.5e-211
Identity = 376/495 (75.96%), Postives = 413/495 (83.43%), Query Frame = 0

Query: 1   MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60
           MGFHQI ERNQ LLSKIA++DGHGE+SPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL
Sbjct: 1   MGFHQIGERNQVLLSKIAINDGHGEDSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60

Query: 61  SFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFD 120
           S  +IVDW+RKHP+ASICT EGLE FK IAN+QDYHGL EFRNAIASFMGK RGGRVKFD
Sbjct: 61  SSDLIVDWIRKHPKASICTDEGLESFKVIANYQDYHGLLEFRNAIASFMGKARGGRVKFD 120

Query: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNN 180
           PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRD++WRTRAQII VHC+SSNN
Sbjct: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDLQWRTRAQIIPVHCHSSNN 180

Query: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240
           FQ+ K ALE AYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLK+LV FVN ++IHLICD
Sbjct: 181 FQIRKEALEAAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKSLVNFVNDNNIHLICD 240

Query: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR 300
           EIYSATVFK+P+FTSIAEI+E+MEHC KELIHILYSLSKDMGLPGFRVGI+YSYND VV 
Sbjct: 241 EIYSATVFKSPSFTSIAEIIEEMEHCNKELIHILYSLSKDMGLPGFRVGILYSYNDNVVS 300

Query: 301 RAR----------QIVELWSSLVPRLNICSPPFAFPTRGLCRQIS--CRELEAPRGEACK 360
             R          Q   L ++++          A  ++ L ++     +ELE   G  C 
Sbjct: 301 TGRKMSSFGLVSSQTQHLLAAMLSDEEFVDNFLAENSKRLAKRHHRFTKELE-KMGITCL 360

Query: 361 AMLEFLCGWI-YGGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANM 420
                L  W+     LKDQ+FK+EMELWRVIINEVKLNVSPGSSFHV EPGWFRVCFANM
Sbjct: 361 NGNAGLFVWMDLRKLLKDQSFKSEMELWRVIINEVKLNVSPGSSFHVTEPGWFRVCFANM 420

Query: 421 DDNTVDVALNRIHSFVKNIDKKKDN-TVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNS 480
           DDNTVDVAL+RIHSFV   DKK+DN T+ MP KT+RRENKLRL+FSFSG RY+EGNVLNS
Sbjct: 421 DDNTVDVALDRIHSFVGKNDKKEDNITLTMPPKTKRRENKLRLNFSFSGGRYEEGNVLNS 480

Query: 481 PHTMSPHSPLVIAKN 482
           PH MSPHSPLV A N
Sbjct: 481 PHMMSPHSPLVRAHN 494

BLAST of Carg26027 vs. ExPASy TrEMBL
Match: Q9MB86 (1-aminocyclopropane-1-carboxylate synthase OS=Cucumis melo OX=3656 GN=CMe-ACS1 PE=3 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 2.1e-208
Identity = 366/493 (74.24%), Postives = 409/493 (82.96%), Query Frame = 0

Query: 1   MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60
           MGFHQ  E NQ LLSKIA+ DGHGE+SPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL
Sbjct: 1   MGFHQTHETNQVLLSKIAISDGHGEDSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60

Query: 61  SFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFD 120
           S  +IVDW+RKHP+ASICT EGL  FK IANFQDYHGLPEFRNAIASFMGK RGGRVKFD
Sbjct: 61  SPDLIVDWIRKHPKASICTDEGLLNFKDIANFQDYHGLPEFRNAIASFMGKARGGRVKFD 120

Query: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNN 180
           PSRIVMGGGATGASETVIFCLADPGDAFL+PSPYYAAFDRD++WRTRAQII VHCNSSNN
Sbjct: 121 PSRIVMGGGATGASETVIFCLADPGDAFLIPSPYYAAFDRDLQWRTRAQIIPVHCNSSNN 180

Query: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240
           FQ+T+ ALE+AYKKA+E+NIKVKG+IITNPSNPLGT YDRDTLK+LV FVN ++IHLICD
Sbjct: 181 FQITREALEVAYKKAEESNIKVKGLIITNPSNPLGTIYDRDTLKSLVNFVNDNNIHLICD 240

Query: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR 300
           EIYSATVFK+P+FTSIAEI+E+M+HCKKELIHIL SLSKDMGLPGFRVGI+YSYND VV 
Sbjct: 241 EIYSATVFKSPSFTSIAEIIEEMDHCKKELIHILSSLSKDMGLPGFRVGILYSYNDTVVS 300

Query: 301 RAR----------QIVELWSSLVPRLNICSPPFAFPTRGLCRQIS--CRELEAPRGEACK 360
            AR          Q   L ++++             ++ L ++ +    ELE   G  C 
Sbjct: 301 IARKMSSFGLVSSQTQHLLAAMLSDEEFVDNFLVENSKRLAKRHARFTEELE-KMGITCL 360

Query: 361 AMLEFLCGWI-YGGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANM 420
                L  W+     LKDQ+F++EMELWRVIINEVKLNVSPGSSFHV EPGWFRVCFANM
Sbjct: 361 NSNAGLFVWMDLRKLLKDQSFESEMELWRVIINEVKLNVSPGSSFHVTEPGWFRVCFANM 420

Query: 421 DDNTVDVALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP 480
           DDNTV VAL+RIHSFV  IDKK+++T+ MP K + RENKLRLSFSFSGRRY+EGNVL SP
Sbjct: 421 DDNTVGVALDRIHSFVGKIDKKENSTIPMPPKKKHRENKLRLSFSFSGRRYEEGNVLKSP 480

BLAST of Carg26027 vs. ExPASy TrEMBL
Match: Q42668 (1-aminocyclopropane-1-carboxylate synthase OS=Cucumis melo OX=3656 GN=CMe-ACS1 (ME-ACS1) PE=2 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 3.9e-207
Identity = 365/493 (74.04%), Postives = 408/493 (82.76%), Query Frame = 0

Query: 1   MGFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60
           MGFHQ  E NQ LLSKIA+ DGHGE+SPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL
Sbjct: 1   MGFHQTHETNQVLLSKIAISDGHGEDSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQL 60

Query: 61  SFGMIVDWLRKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFD 120
           S  +IVDW+RKHP+ASI T EGL  FK IANFQDYHGLPEFRNAIASFMGK RGGRVKFD
Sbjct: 61  SPDLIVDWIRKHPKASIYTDEGLLNFKDIANFQDYHGLPEFRNAIASFMGKARGGRVKFD 120

Query: 121 PSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNN 180
           PSRIVMGGGATGASETVIFCLADPGDAFL+PSPYYAAFDRD++WRTRAQII VHCNSSNN
Sbjct: 121 PSRIVMGGGATGASETVIFCLADPGDAFLIPSPYYAAFDRDLQWRTRAQIIPVHCNSSNN 180

Query: 181 FQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICD 240
           FQ+T+ ALE+AYKKA+E+NIKVKG+IITNPSNPLGT YDRDTLK+LV FVN ++IHLICD
Sbjct: 181 FQITREALEVAYKKAEESNIKVKGLIITNPSNPLGTIYDRDTLKSLVNFVNDNNIHLICD 240

Query: 241 EIYSATVFKAPTFTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVR 300
           EIYSATVFK+P+FTSIAEI+E+M+HCKKELIHIL SLSKDMGLPGFRVGI+YSYND VV 
Sbjct: 241 EIYSATVFKSPSFTSIAEIIEEMDHCKKELIHILSSLSKDMGLPGFRVGILYSYNDTVVS 300

Query: 301 RAR----------QIVELWSSLVPRLNICSPPFAFPTRGLCRQIS--CRELEAPRGEACK 360
            AR          Q   L ++++             ++ L ++ +    ELE   G  C 
Sbjct: 301 IARKMSSFGLVSSQTQHLLAAMLSDEEFVDNFLVENSKRLAKRHARFTEELE-KMGITCL 360

Query: 361 AMLEFLCGWI-YGGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANM 420
                L  W+     LKDQ+F++EMELWRVIINEVKLNVSPGSSFHV EPGWFRVCFANM
Sbjct: 361 NSNAGLFVWMDLRKLLKDQSFESEMELWRVIINEVKLNVSPGSSFHVTEPGWFRVCFANM 420

Query: 421 DDNTVDVALNRIHSFVKNIDKKKDNTVAMPSKTRRRENKLRLSFSFSGRRYDEGNVLNSP 480
           DDNTV VAL+RIHSFV  IDKK+++T+ MP K + RENKLRLSFSFSGRRY+EGNVL SP
Sbjct: 421 DDNTVGVALDRIHSFVGKIDKKENSTIPMPPKKKHRENKLRLSFSFSGRRYEEGNVLKSP 480

BLAST of Carg26027 vs. TAIR 10
Match: AT3G61510.1 (ACC synthase 1 )

HSP 1 Score: 526.9 bits (1356), Expect = 1.7e-149
Identity = 273/486 (56.17%), Postives = 346/486 (71.19%), Query Frame = 0

Query: 13  LLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQLSFGMIVDWLRKH 72
           LLSK+A+ D HGE SPYF GWKAYDN+PFHP +NP GVIQMGLAENQL   +I +W++++
Sbjct: 10  LLSKLALSDKHGEASPYFHGWKAYDNNPFHPTHNPQGVIQMGLAENQLCSDLIKEWIKEN 69

Query: 73  PEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFDPSRIVMGGGATG 132
           P+ASICT EG++ F  IA FQDYHGL +FR AIA+FM + RGGRV+F+  R+VM GGATG
Sbjct: 70  PQASICTAEGIDSFSDIAVFQDYHGLKQFRQAIATFMERARGGRVRFEAERVVMSGGATG 129

Query: 133 ASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNNFQVTKAALEIAY 192
           A+ET++FCLADPGDAFLVP+PYYAAFDRD++WRT  +II V C+SSNNFQ+TK ALE AY
Sbjct: 130 ANETIMFCLADPGDAFLVPTPYYAAFDRDLRWRTGVRIIPVECSSSNNFQITKQALESAY 189

Query: 193 KKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICDEIYSATVFKAPT 252
            KAQE  IK+KG+II   SNPLGT+ DR+TL++LV+F+N   IHL+CDEIY+ATVF  P 
Sbjct: 190 LKAQETGIKIKGLII---SNPLGTSLDRETLESLVSFINDKQIHLVCDEIYAATVFAEPG 249

Query: 253 FTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVRRARQI------- 312
           F S+AEI+++M +  ++LIHI+YSLSKDMGLPGFRVG++YSYND+VV  AR++       
Sbjct: 250 FISVAEIIQEMYYVNRDLIHIVYSLSKDMGLPGFRVGVVYSYNDVVVSCARRMSSFGLVS 309

Query: 313 VELWSSLVPRLNICSPPFAFPTRGLCRQISCRE------LEAPRGEACKAMLEFLCGWIY 372
            +  S L   L+  S    F    + ++++ R       LE       ++          
Sbjct: 310 SQTQSFLAAMLSDQSFVDNFLVE-VSKRVAKRHHMFTEGLEEMGISCLRSNAGLFVLMDL 369

Query: 373 GGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANMDDNTVDVALNRI 432
              LKDQTF +EM LWRVIIN+VK+NVSPGSSFH +EPGWFRVCFANMD++T+ +AL RI
Sbjct: 370 RHMLKDQTFDSEMALWRVIINKVKINVSPGSSFHCSEPGWFRVCFANMDEDTLQIALERI 429

Query: 433 HSFVKNIDKKKDNTVAMPSKTRRRENKLRLSF------SFSGRRYDEGNVLNSPHTMSPH 480
             FV   D+   N         +RENK R SF      S S  RY+E   + SP  MSPH
Sbjct: 430 KDFVVG-DRANKNKNCNCICNNKRENKKRKSFQKNLKLSLSSMRYEEH--VRSPKLMSPH 488

BLAST of Carg26027 vs. TAIR 10
Match: AT1G01480.1 (1-amino-cyclopropane-1-carboxylate synthase 2 )

HSP 1 Score: 513.1 bits (1320), Expect = 2.5e-145
Identity = 271/492 (55.08%), Postives = 343/492 (69.72%), Query Frame = 0

Query: 12  ALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQLSFGMIVDWLRK 71
           A+LSKIA ++ HGENS YFDGWKAYD DPFH   NP G+IQMGLAENQL   +I DW+++
Sbjct: 10  AVLSKIATNNQHGENSEYFDGWKAYDKDPFHLSRNPHGIIQMGLAENQLCLDLIKDWVKE 69

Query: 72  HPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFDPSRIVMGGGAT 131
           +PEASICT EG+ +F  IANFQDYHGL +FR AIA FMGK RGGRV FDP R+VM GGAT
Sbjct: 70  NPEASICTLEGIHQFSDIANFQDYHGLKKFRQAIAHFMGKARGGRVTFDPERVVMSGGAT 129

Query: 132 GASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNNFQVTKAALEIA 191
           GA+ET++FCLADPGD FL+PSPYYAAFDRD++WRT  +II V C+SS+NF++T  A E A
Sbjct: 130 GANETIMFCLADPGDVFLIPSPYYAAFDRDLRWRTGVEIIPVPCSSSDNFKLTVDAAEWA 189

Query: 192 YKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICDEIYSATVFKAP 251
           YKKAQE+N KVKG+I+TNPSNPLGT  D+DTL  LV FV + +IHL+ DEIY+ATVF   
Sbjct: 190 YKKAQESNKKVKGLILTNPSNPLGTMLDKDTLTNLVRFVTRKNIHLVVDEIYAATVFAGG 249

Query: 252 TFTSIAEIVEQME--HCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVRRARQ----- 311
            F S+AE+V  ++      +LIHI+YSLSKDMGLPGFRVGI+YS+ND VV  AR+     
Sbjct: 250 DFVSVAEVVNDVDISEVNVDLIHIVYSLSKDMGLPGFRVGIVYSFNDSVVSCARKMSSFG 309

Query: 312 IVELWSSLVPRLNICSPPFAFP-TRGLCRQISCRELEAPRG-----EACKAMLEFLCGWI 371
           +V   + L+    +    F         R++  R      G      AC      L  W+
Sbjct: 310 LVSSQTQLMLASMLSDDQFVDNFLMESSRRLGIRHKVFTTGIKKADIACLTSNAGLFAWM 369

Query: 372 -YGGYLKDQ-TFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANMDDNTVDVAL 431
                L+D+ +F++E+ELW +II+ VKLNVSPGSSF   EPGWFR+CFANMDD+T+ VAL
Sbjct: 370 DLRHLLRDRNSFESEIELWHIIIDRVKLNVSPGSSFRCTEPGWFRICFANMDDDTLHVAL 429

Query: 432 NRIHSFV-----KNIDKKKDNTVAMPSKTRRR----ENKLRLSFSFSGRRYDEGNVLNSP 480
            RI  FV     K ++K  +N   + +K+ ++    +  LRLSF    R Y++G  L+SP
Sbjct: 430 GRIQDFVSKNKNKIVEKASENDQVIQNKSAKKLKWTQTNLRLSFR---RLYEDG--LSSP 489

BLAST of Carg26027 vs. TAIR 10
Match: AT4G11280.1 (1-aminocyclopropane-1-carboxylic acid (acc) synthase 6 )

HSP 1 Score: 508.4 bits (1308), Expect = 6.1e-144
Identity = 265/485 (54.64%), Postives = 330/485 (68.04%), Query Frame = 0

Query: 13  LLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQLSFGMIVDWLRKH 72
           LLSKIA  DGHGENS YFDGWKAY+ +PFHP + P GVIQMGLAENQL   ++  W+ KH
Sbjct: 14  LLSKIASGDGHGENSSYFDGWKAYEENPFHPIDRPDGVIQMGLAENQLCGDLMRKWVLKH 73

Query: 73  PEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFDPSRIVMGGGATG 132
           PEASICT EG+ +F  IA FQDYHGLPEFR A+A FM K R  +VKFDP RIVM GGATG
Sbjct: 74  PEASICTSEGVNQFSDIAIFQDYHGLPEFRQAVAKFMEKTRNNKVKFDPDRIVMSGGATG 133

Query: 133 ASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNNFQVTKAALEIAY 192
           A ETV FCLA+PGD FLVP+PYY  FDRD++WRT   ++ V C+SSN F++T  ALE AY
Sbjct: 134 AHETVAFCLANPGDGFLVPTPYYPGFDRDLRWRTGVNLVPVTCHSSNGFKITVEALEAAY 193

Query: 193 KKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICDEIYSATVFKAPT 252
           + A+++NI VKG+++TNPSNPLGTT DR+ LK+LV F N   IHLI DEIY+AT F    
Sbjct: 194 ENARKSNIPVKGLLVTNPSNPLGTTLDRECLKSLVNFTNDKGIHLIADEIYAATTFGQSE 253

Query: 253 FTSIAEIVEQMEHCKKELIHILYSLSKDMGLPGFRVGIIYSYNDLVVRRARQIVE--LWS 312
           F S+AE++E++E C ++LIHI+YSLSKDMGLPG RVGI+YSYND VV+ AR++    L S
Sbjct: 254 FISVAEVIEEIEDCNRDLIHIVYSLSKDMGLPGLRVGIVYSYNDRVVQIARKMSSFGLVS 313

Query: 313 SLVPRLNICSPPFAFPTRGLCRQISCRELEAPRGEACKAMLEFLCGWIYG---------- 372
           S    L               R+   R L A   E    +     GW+            
Sbjct: 314 SQTQHLIAKMLSDEEFVDEFIRESKLR-LAARHAEITTGLDGLGIGWLKAKAGLFLWMDL 373

Query: 373 -GYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANMDDNTVDVALNRI 432
              LK  TF +E ELWRVI+++VKLNVSPG SFH +EPGWFRVCFANMD  T++ AL RI
Sbjct: 374 RNLLKTATFDSETELWRVIVHQVKLNVSPGGSFHCHEPGWFRVCFANMDHKTMETALERI 433

Query: 433 HSFVKNIDKKKDNTVA--MPSKTRRR--ENKLRLSFSFSGRRYDEGNVLNSPHTMSPHSP 481
             F   ++++     A  M +K +++  ++ LRLSFS   RR+D+G    SPH+  P SP
Sbjct: 434 RVFTSQLEEETKPMAATTMMAKKKKKCWQSNLRLSFS-DTRRFDDG--FFSPHSPVPPSP 493

BLAST of Carg26027 vs. TAIR 10
Match: AT4G26200.1 (1-amino-cyclopropane-1-carboxylate synthase 7 )

HSP 1 Score: 433.0 bits (1112), Expect = 3.3e-121
Identity = 222/428 (51.87%), Postives = 295/428 (68.93%), Query Frame = 0

Query: 10  NQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQLSFGMIVDWL 69
           N   LS++AV D HGE+SPYF GWKAYD +P+   +NP GVIQMGLAENQ+SF ++  +L
Sbjct: 14  NNVELSRVAVSDTHGEDSPYFAGWKAYDENPYDESHNPSGVIQMGLAENQVSFDLLETYL 73

Query: 70  -RKHPEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFDPSRIVMGG 129
            +K+PE S+   +G   F+  A FQDYHGL  FR A+ASFM +IRGG+ +FDP RIV+  
Sbjct: 74  EKKNPEGSMWGSKGAPGFRENALFQDYHGLKTFRQAMASFMEQIRGGKARFDPDRIVLTA 133

Query: 130 GATGASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNNFQVTKAAL 189
           GAT A+E + F LADP DA LVP+PYY  FDRD++WRT  +I+ +HC+SSN+FQ+T  AL
Sbjct: 134 GATAANELLTFILADPNDALLVPTPYYPGFDRDLRWRTGVKIVPIHCDSSNHFQITPEAL 193

Query: 190 EIAYKKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICDEIYSATVF 249
           E AY+ A++ANI+V+GV+ITNPSNPLG T  +  L+ L+ F  + +IHL+ DEIYS +VF
Sbjct: 194 ESAYQTARDANIRVRGVLITNPSNPLGATVQKKVLEDLLDFCVRKNIHLVSDEIYSGSVF 253

Query: 250 KAPTFTSIAEIVEQMEHCK-KELIHILYSLSKDMGLPGFRVGIIYSYNDLVVRRARQI-- 309
            A  FTS+AEIVE ++    KE +HI+YSLSKD+GLPGFRVG IYSYND VVR AR++  
Sbjct: 254 HASEFTSVAEIVENIDDVSVKERVHIVYSLSKDLGLPGFRVGTIYSYNDNVVRTARRMSS 313

Query: 310 VELWSSLVPRL---NICSPPFAFPTRGLCRQISCRELEAPRGEACKAMLEFLCG------ 369
             L SS    +    +    F      + R+   R  +       KA +E L G      
Sbjct: 314 FTLVSSQTQHMLASMLSDEEFTEKYIRINRERLRRRYDTIVEGLKKAGIECLKGNAGLFC 373

Query: 370 WIYGGYLKDQTFK-AEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANMDDNTVDVA 424
           W+  G+L ++  K  E++LW VI+ E+ LN+SPGSS H +E GWFRVCFANM +NT+++A
Sbjct: 374 WMNLGFLLEKKTKDGELQLWDVILKELNLNISPGSSCHCSEVGWFRVCFANMSENTLEIA 433

BLAST of Carg26027 vs. TAIR 10
Match: AT4G37770.1 (1-amino-cyclopropane-1-carboxylate synthase 8 )

HSP 1 Score: 422.9 bits (1086), Expect = 3.4e-118
Identity = 221/449 (49.22%), Postives = 294/449 (65.48%), Query Frame = 0

Query: 13  LLSKIAVDDGHGENSPYFDGWKAYDNDPFHPENNPLGVIQMGLAENQLSFGMIVDWLRKH 72
           LLSK A  + HG++S YF GW+ Y+ +P+    NP G+IQMGLAENQLSF +I  WL K+
Sbjct: 3   LLSKKASCNTHGQDSSYFWGWEEYEKNPYDEIKNPDGIIQMGLAENQLSFDLIESWLAKN 62

Query: 73  PEASICTPEGLERFKSIANFQDYHGLPEFRNAIASFMGKIRGGRVKFDPSRIVMGGGATG 132
           P+A+    EG   F+ +A FQDYHGLP F+NA+A FM + RG RV F+P+++V+  GAT 
Sbjct: 63  PDAANFQREGQSIFRELALFQDYHGLPSFKNAMADFMSENRGNRVSFNPNKLVLTAGATP 122

Query: 133 ASETVIFCLADPGDAFLVPSPYYAAFDRDIKWRTRAQIIRVHCNSSNNFQVTKAALEIAY 192
           A+ET++FCLADPGDAFL+P+PYY  FDRD+KWRT A+I+ + C S+N F++TK ALE AY
Sbjct: 123 ANETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIQCKSANGFRITKVALEEAY 182

Query: 193 KKAQEANIKVKGVIITNPSNPLGTTYDRDTLKTLVTFVNQHDIHLICDEIYSATVFKAPT 252
           ++AQ+ N+KVKGV+ITNPSNPLGTT  R  L  L+ F+++  IHLI DEIYS TVF  P 
Sbjct: 183 EQAQKLNLKVKGVLITNPSNPLGTTTTRTELNHLLDFISRKKIHLISDEIYSGTVFTNPG 242

Query: 253 FTSIAEIVEQMEHCKKEL---IHILYSLSKDMGLPGFRVGIIYSYNDLVVRRARQIVE-- 312
           F S+ E+++  +    ++   +HI+YSLSKD+GLPGFRVG+IYS +D VV  A ++    
Sbjct: 243 FISVMEVLKDRKLENTDVFDRVHIVYSLSKDLGLPGFRVGVIYSNDDFVVSAATKMSSFG 302

Query: 313 LWSSLVPRLNICSPPFAFPTRGLCRQISCR----------ELEAPRGEACKAMLEFLCGW 372
           L SS    L          T+    +   R           LEA   E  K+     C  
Sbjct: 303 LISSQTQYLLSALLSDKTFTKNYLEENQIRLKNRHKKLVSGLEAAGIECLKSNAGLFCWV 362

Query: 373 IYGGYLKDQTFKAEMELWRVIINEVKLNVSPGSSFHVNEPGWFRVCFANMDDNTVDVALN 432
                LK  TF+AE+ELW+ I+ EVKLN+SPGSS H NEPGWFRVCFAN+ + T+ VAL+
Sbjct: 363 DMRHLLKSNTFEAEIELWKKIVYEVKLNISPGSSCHCNEPGWFRVCFANLSEETLKVALD 422

Query: 433 RIHSFVKNIDKKKDNTVAMPSKTRRRENK 447
           R+  FV             PS TRR +++
Sbjct: 423 RLKRFVDG-----------PSPTRRSQSE 440

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7019125.12.7e-287100.001-aminocyclopropane-1-carboxylate synthase CMW33, partial [Cucurbita argyrosperm... [more]
KAG6575198.18.0e-23985.021-aminocyclopropane-1-carboxylate synthase CMW33, partial [Cucurbita argyrosperm... [more]
XP_023547768.12.0e-23784.211-aminocyclopropane-1-carboxylate synthase 1 [Cucurbita pepo subsp. pepo][more]
P23279.13.4e-23784.01RecName: Full=1-aminocyclopropane-1-carboxylate synthase 1; Short=ACC synthase 1... [more]
AAA33111.14.4e-23784.011-aminocyclopropane-1-carboxylate synthase [Cucurbita pepo][more]
Match NameE-valueIdentityDescription
P232794.4e-24084.011-aminocyclopropane-1-carboxylate synthase 1 OS=Cucurbita pepo OX=3663 GN=ACC1A ... [more]
P235992.2e-23984.011-aminocyclopropane-1-carboxylate synthase CMW33 OS=Cucurbita maxima OX=3661 GN=... [more]
Q003792.5e-23582.761-aminocyclopropane-1-carboxylate synthase 2 OS=Cucurbita pepo OX=3663 GN=ACS2 P... [more]
Q9MB953.7e-16260.331-aminocyclopropane-1-carboxylate synthase 1 OS=Prunus mume OX=102107 GN=ACS1 PE... [more]
Q072621.7e-15958.181-aminocyclopropane-1-carboxylate synthase OS=Nicotiana tabacum OX=4097 GN=ACS1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1KY378.1e-23784.011-aminocyclopropane-1-carboxylate synthase CMW33 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1H4276.9e-23684.211-aminocyclopropane-1-carboxylate synthase 1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
B2WR814.5e-21175.961-aminocyclopropane-1-carboxylate synthase OS=Citrullus colocynthis x Citrullus ... [more]
Q9MB862.1e-20874.241-aminocyclopropane-1-carboxylate synthase OS=Cucumis melo OX=3656 GN=CMe-ACS1 P... [more]
Q426683.9e-20774.041-aminocyclopropane-1-carboxylate synthase OS=Cucumis melo OX=3656 GN=CMe-ACS1 (... [more]
Match NameE-valueIdentityDescription
AT3G61510.11.7e-14956.17ACC synthase 1 [more]
AT1G01480.12.5e-14555.081-amino-cyclopropane-1-carboxylate synthase 2 [more]
AT4G11280.16.1e-14454.641-aminocyclopropane-1-carboxylic acid (acc) synthase 6 [more]
AT4G26200.13.3e-12151.871-amino-cyclopropane-1-carboxylate synthase 7 [more]
AT4G37770.13.4e-11849.221-amino-cyclopropane-1-carboxylate synthase 8 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00753ACCSYNTHASEcoord: 123..143
score: 68.31
coord: 199..223
score: 71.67
coord: 53..79
score: 61.51
coord: 145..166
score: 76.08
coord: 86..102
score: 76.47
coord: 270..294
score: 82.03
coord: 235..258
score: 67.14
NoneNo IPR availablePANTHERPTHR43795:SF61-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE 6coord: 363..435
NoneNo IPR availablePANTHERPTHR43795:SF61-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE 6coord: 8..305
NoneNo IPR availablePANTHERPTHR43795BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATEDcoord: 363..435
coord: 8..305
NoneNo IPR availableCDDcd00609AAT_likecoord: 91..420
e-value: 2.72082E-51
score: 175.993
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 73..306
e-value: 1.7E-111
score: 374.7
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 357..423
e-value: 2.1E-23
score: 84.9
coord: 39..72
e-value: 1.7E-111
score: 374.7
IPR004839Aminotransferase, class I/classIIPFAMPF00155Aminotran_1_2coord: 51..310
e-value: 1.2E-70
score: 238.5
coord: 370..419
e-value: 3.1E-11
score: 43.1
IPR004838Aminotransferases, class-I, pyridoxal-phosphate-binding sitePROSITEPS00105AA_TRANSFER_CLASS_1coord: 276..289
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 23..424

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg26027-RACarg26027-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042218 1-aminocyclopropane-1-carboxylate biosynthetic process
biological_process GO:0009693 ethylene biosynthetic process
biological_process GO:0009835 fruit ripening
biological_process GO:0009058 biosynthetic process
molecular_function GO:0016847 1-aminocyclopropane-1-carboxylate synthase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0008483 transaminase activity
molecular_function GO:0003824 catalytic activity