Carg25786 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg25786 vs. NCBI nr
Match: KAG7027265.1 (Ethylene-insensitive protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2587.0 bits (6704), Expect = 0.0e+00
Identity = 1333/1333 (100.00%), Postives = 1333/1333 (100.00%), Query Frame = 0

Query: 1    MPLRLLELMTKVETIMDFPGFVSQALTLPYWSILWEIPINTMESMTSHTNHLSGTIHRFM 60
            MPLRLLELMTKVETIMDFPGFVSQALTLPYWSILWEIPINTMESMTSHTNHLSGTIHRFM
Sbjct: 1    MPLRLLELMTKVETIMDFPGFVSQALTLPYWSILWEIPINTMESMTSHTNHLSGTIHRFM 60

Query: 61   PFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFNLAAILCQYLSASIGVVTGR 120
            PFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFNLAAILCQYLSASIGVVTGR
Sbjct: 61   PFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFNLAAILCQYLSASIGVVTGR 120

Query: 121  GLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGLNLLLGWDLFTCVLLTGVVA 180
            GLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGLNLLLGWDLFTCVLLTGVVA
Sbjct: 121  GLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGLNLLLGWDLFTCVLLTGVVA 180

Query: 181  ALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEIPLSMNLMLTRLNGESAFTL 240
            ALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEIPLSMNLMLTRLNGESAFTL
Sbjct: 181  ALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEIPLSMNLMLTRLNGESAFTL 240

Query: 241  MSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLFAIVCIFSGIYVVNNVLMNS 300
            MSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLFAIVCIFSGIYVVNNVLMNS
Sbjct: 241  MSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLFAIVCIFSGIYVVNNVLMNS 300

Query: 301  AANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFLSNQITALTWSLGGQLVLTN 360
            AANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFLSNQITALTWSLGGQLVLTN
Sbjct: 301  AANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFLSNQITALTWSLGGQLVLTN 360

Query: 361  FLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIFSQVMVALLLPSSVIPLFRV 420
            FLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIFSQVMVALLLPSSVIPLFRV
Sbjct: 361  FLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIFSQVMVALLLPSSVIPLFRV 420

Query: 421  ASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFGNSDWVVNLRWNMGSGLSIP 480
            ASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFGNSDWVVNLRWNMGSGLSIP
Sbjct: 421  ASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFGNSDWVVNLRWNMGSGLSIP 480

Query: 481  YVFFLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDVPEVKPDSAVEREGIDLGKG 540
            YVFFLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDVPEVKPDSAVEREGIDLGKG
Sbjct: 481  YVFFLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDVPEVKPDSAVEREGIDLGKG 540

Query: 541  SYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGETQSSSEVPSSPKYVSENLIS 600
            SYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGETQSSSEVPSSPKYVSENLIS
Sbjct: 541  SYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGETQSSSEVPSSPKYVSENLIS 600

Query: 601  TELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGDLRSEKDSYEVDNWEAEEPL 660
            TELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGDLRSEKDSYEVDNWEAEEPL
Sbjct: 601  TELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGDLRSEKDSYEVDNWEAEEPL 660

Query: 661  KEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGLGRAARRQLTAILDEFWGQL 720
            KEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGLGRAARRQLTAILDEFWGQL
Sbjct: 661  KEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGLGRAARRQLTAILDEFWGQL 720

Query: 721  YDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSPYSSPLGSKASNPISSGLYD 780
            YDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSPYSSPLGSKASNPISSGLYD
Sbjct: 721  YDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSPYSSPLGSKASNPISSGLYD 780

Query: 781  SPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSHNGLDSGVKRYSSLRSLPSS 840
            SPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSHNGLDSGVKRYSSLRSLPSS
Sbjct: 781  SPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSHNGLDSGVKRYSSLRSLPSS 840

Query: 841  ESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKYHTLGGAGGASLRDSVAFAM 900
            ESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKYHTLGGAGGASLRDSVAFAM
Sbjct: 841  ESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKYHTLGGAGGASLRDSVAFAM 900

Query: 901  GQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPSGTGENLSSVSNTKKYHSLP 960
            GQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPSGTGENLSSVSNTKKYHSLP
Sbjct: 901  GQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPSGTGENLSSVSNTKKYHSLP 960

Query: 961  DIHRDQHISDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSRSVAPLAFNELSSSSVYGGA 1020
            DIHRDQHISDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSRSVAPLAFNELSSSSVYGGA
Sbjct: 961  DIHRDQHISDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSRSVAPLAFNELSSSSVYGGA 1020

Query: 1021 LSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHTISQEASFVVNSEARLLQSF 1080
            LSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHTISQEASFVVNSEARLLQSF
Sbjct: 1021 LSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHTISQEASFVVNSEARLLQSF 1080

Query: 1081 RKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEAETRVVRMKEPPPFSPDRRS 1140
            RKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEAETRVVRMKEPPPFSPDRRS
Sbjct: 1081 RKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEAETRVVRMKEPPPFSPDRRS 1140

Query: 1141 GSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIHRILDLSLMESRPELWGKYT 1200
            GSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIHRILDLSLMESRPELWGKYT
Sbjct: 1141 GSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIHRILDLSLMESRPELWGKYT 1200

Query: 1201 YVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQIANGMLPPAAKPGKGKSTT 1260
            YVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQIANGMLPPAAKPGKGKSTT
Sbjct: 1201 YVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQIANGMLPPAAKPGKGKSTT 1260

Query: 1261 AAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASVLKRYKRRLSNKPVATLEVS 1320
            AAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASVLKRYKRRLSNKPVATLEVS
Sbjct: 1261 AAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASVLKRYKRRLSNKPVATLEVS 1320

Query: 1321 RKLPAAASVPYSS 1334
            RKLPAAASVPYSS
Sbjct: 1321 RKLPAAASVPYSS 1333

BLAST of Carg25786 vs. NCBI nr
Match: KAG6595248.1 (Ethylene-insensitive protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2500.3 bits (6479), Expect = 0.0e+00
Identity = 1289/1292 (99.77%), Postives = 1290/1292 (99.85%), Query Frame = 0

Query: 42   MESMTSHTNHLSGTIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 101
            MESMTSHTNHLSGTIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN
Sbjct: 1    MESMTSHTNHLSGTIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 60

Query: 102  LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 161
            LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL
Sbjct: 61   LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 120

Query: 162  NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 221
            NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI
Sbjct: 121  NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 180

Query: 222  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF 281
            PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF
Sbjct: 181  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF 240

Query: 282  AIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL 341
            AIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL
Sbjct: 241  AIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL 300

Query: 342  SNQITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF 401
            SNQITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF
Sbjct: 301  SNQITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF 360

Query: 402  SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG 461
            SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG
Sbjct: 361  SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG 420

Query: 462  NSDWVVNLRWNMGSGLSIPYVFFLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV 521
            NSDWVVNLRWNMGSGLSIPYVF LI ACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV
Sbjct: 421  NSDWVVNLRWNMGSGLSIPYVFLLINACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV 480

Query: 522  PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET 581
            PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET
Sbjct: 481  PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET 540

Query: 582  QSSSEVPSSPKYVSENLISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGD 641
            QSSSEVPSSPKYVSENLISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGD
Sbjct: 541  QSSSEVPSSPKYVSENLISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGD 600

Query: 642  LRSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGL 701
            LRSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGL
Sbjct: 601  LRSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGL 660

Query: 702  GRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSP 761
            GRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSP
Sbjct: 661  GRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSP 720

Query: 762  YSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSH 821
            YSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSH
Sbjct: 721  YSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSH 780

Query: 822  NGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKY 881
            NGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKY
Sbjct: 781  NGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKY 840

Query: 882  HTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPS 941
            HTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPS
Sbjct: 841  HTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPS 900

Query: 942  GTGENLSSVSNTKKYHSLPDIHRDQHISDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSR 1001
            GTGENLSSVSNTKKYHSLPDIHRDQH+SDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSR
Sbjct: 901  GTGENLSSVSNTKKYHSLPDIHRDQHVSDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSR 960

Query: 1002 SVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHT 1061
            SVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHT
Sbjct: 961  SVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHT 1020

Query: 1062 ISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEA 1121
            ISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEA
Sbjct: 1021 ISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEA 1080

Query: 1122 ETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIH 1181
            ETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIH
Sbjct: 1081 ETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIH 1140

Query: 1182 RILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQ 1241
            RILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQ
Sbjct: 1141 RILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQ 1200

Query: 1242 IANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASV 1301
            IANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASV
Sbjct: 1201 IANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASV 1260

Query: 1302 LKRYKRRLSNKPVATLEVSRKLPAAASVPYSS 1334
            LKRYKRRLSNKPVATLEVSRKLPAAASVPYSS
Sbjct: 1261 LKRYKRRLSNKPVATLEVSRKLPAAASVPYSS 1292

BLAST of Carg25786 vs. NCBI nr
Match: XP_022963082.1 (ethylene-insensitive protein 2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 2488.0 bits (6447), Expect = 0.0e+00
Identity = 1282/1292 (99.23%), Postives = 1286/1292 (99.54%), Query Frame = 0

Query: 42   MESMTSHTNHLSGTIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 101
            MESMTSHTNHLSG IHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN
Sbjct: 1    MESMTSHTNHLSGAIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 60

Query: 102  LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 161
            LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL
Sbjct: 61   LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 120

Query: 162  NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 221
            NLLLGWDLFTCVLLTGVVA LFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI
Sbjct: 121  NLLLGWDLFTCVLLTGVVAGLFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 180

Query: 222  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF 281
            PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF
Sbjct: 181  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF 240

Query: 282  AIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL 341
            AIVCIFSGIY+VNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL
Sbjct: 241  AIVCIFSGIYLVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL 300

Query: 342  SNQITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF 401
            SN ITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF
Sbjct: 301  SNHITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF 360

Query: 402  SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG 461
            SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG
Sbjct: 361  SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG 420

Query: 462  NSDWVVNLRWNMGSGLSIPYVFFLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV 521
            NSDWVVNLRWNMGSGLSIPYV  LITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV
Sbjct: 421  NSDWVVNLRWNMGSGLSIPYVVLLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV 480

Query: 522  PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET 581
            PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET
Sbjct: 481  PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET 540

Query: 582  QSSSEVPSSPKYVSENLISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGD 641
            QSSSEVPSSPKYVSEN+ISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGD
Sbjct: 541  QSSSEVPSSPKYVSENVISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGD 600

Query: 642  LRSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGL 701
            L SEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGL
Sbjct: 601  LPSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGL 660

Query: 702  GRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSP 761
            GRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSP
Sbjct: 661  GRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSP 720

Query: 762  YSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSH 821
            YSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSH
Sbjct: 721  YSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSH 780

Query: 822  NGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKY 881
            NGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKY
Sbjct: 781  NGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKY 840

Query: 882  HTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPS 941
            HTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPS
Sbjct: 841  HTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPS 900

Query: 942  GTGENLSSVSNTKKYHSLPDIHRDQHISDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSR 1001
            GTGENLSSVSNTKKYHSLPDIHRDQH+SDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSR
Sbjct: 901  GTGENLSSVSNTKKYHSLPDIHRDQHVSDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSR 960

Query: 1002 SVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHT 1061
            SVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHT
Sbjct: 961  SVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHT 1020

Query: 1062 ISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEA 1121
            ISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEA
Sbjct: 1021 ISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEA 1080

Query: 1122 ETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIH 1181
            ETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIH
Sbjct: 1081 ETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIH 1140

Query: 1182 RILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQ 1241
            RILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQ
Sbjct: 1141 RILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQ 1200

Query: 1242 IANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASV 1301
            IANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASV
Sbjct: 1201 IANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASV 1260

Query: 1302 LKRYKRRLSNKPVATLEVSRKLPAAASVPYSS 1334
            LKRYKRRLSNKPVATLEVSRKLPAA+SVPYSS
Sbjct: 1261 LKRYKRRLSNKPVATLEVSRKLPAASSVPYSS 1292

BLAST of Carg25786 vs. NCBI nr
Match: XP_022963078.1 (ethylene-insensitive protein 2-like isoform X1 [Cucurbita moschata] >XP_022963079.1 ethylene-insensitive protein 2-like isoform X1 [Cucurbita moschata] >XP_022963080.1 ethylene-insensitive protein 2-like isoform X1 [Cucurbita moschata] >XP_022963081.1 ethylene-insensitive protein 2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 2483.0 bits (6434), Expect = 0.0e+00
Identity = 1282/1294 (99.07%), Postives = 1286/1294 (99.38%), Query Frame = 0

Query: 42   MESMTSHTNHLSGTIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 101
            MESMTSHTNHLSG IHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN
Sbjct: 1    MESMTSHTNHLSGAIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 60

Query: 102  LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 161
            LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL
Sbjct: 61   LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 120

Query: 162  NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 221
            NLLLGWDLFTCVLLTGVVA LFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI
Sbjct: 121  NLLLGWDLFTCVLLTGVVAGLFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 180

Query: 222  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIV--QQQQQSPPNISKEVLCNNH 281
            PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIV  QQQQQSPPNISKEVLCNNH
Sbjct: 181  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQQQSPPNISKEVLCNNH 240

Query: 282  LFAIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVL 341
            LFAIVCIFSGIY+VNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVL
Sbjct: 241  LFAIVCIFSGIYLVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVL 300

Query: 342  FLSNQITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLL 401
            FLSN ITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLL
Sbjct: 301  FLSNHITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLL 360

Query: 402  IFSQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMF 461
            IFSQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMF
Sbjct: 361  IFSQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMF 420

Query: 462  FGNSDWVVNLRWNMGSGLSIPYVFFLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNW 521
            FGNSDWVVNLRWNMGSGLSIPYV  LITACSAFCLMLWLAATPLKSATAIAQLDAQVLNW
Sbjct: 421  FGNSDWVVNLRWNMGSGLSIPYVVLLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNW 480

Query: 522  DVPEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQG 581
            DVPEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQG
Sbjct: 481  DVPEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQG 540

Query: 582  ETQSSSEVPSSPKYVSENLISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFD 641
            ETQSSSEVPSSPKYVSEN+ISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFD
Sbjct: 541  ETQSSSEVPSSPKYVSENVISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFD 600

Query: 642  GDLRSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLA 701
            GDL SEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLA
Sbjct: 601  GDLPSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLA 660

Query: 702  GLGRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKD 761
            GLGRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKD
Sbjct: 661  GLGRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKD 720

Query: 762  SPYSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSS 821
            SPYSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSS
Sbjct: 721  SPYSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSS 780

Query: 822  SHNGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSAS 881
            SHNGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSAS
Sbjct: 781  SHNGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSAS 840

Query: 882  KYHTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLS 941
            KYHTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLS
Sbjct: 841  KYHTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLS 900

Query: 942  PSGTGENLSSVSNTKKYHSLPDIHRDQHISDKSSQWDNVTGYGSSISRIPVRGLSYSNSG 1001
            PSGTGENLSSVSNTKKYHSLPDIHRDQH+SDKSSQWDNVTGYGSSISRIPVRGLSYSNSG
Sbjct: 901  PSGTGENLSSVSNTKKYHSLPDIHRDQHVSDKSSQWDNVTGYGSSISRIPVRGLSYSNSG 960

Query: 1002 SRSVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRL 1061
            SRSVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRL
Sbjct: 961  SRSVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRL 1020

Query: 1062 HTISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLY 1121
            HTISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLY
Sbjct: 1021 HTISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLY 1080

Query: 1122 EAETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWC 1181
            EAETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWC
Sbjct: 1081 EAETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWC 1140

Query: 1182 IHRILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSS 1241
            IHRILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSS
Sbjct: 1141 IHRILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSS 1200

Query: 1242 PQIANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLA 1301
            PQIANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLA
Sbjct: 1201 PQIANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLA 1260

Query: 1302 SVLKRYKRRLSNKPVATLEVSRKLPAAASVPYSS 1334
            SVLKRYKRRLSNKPVATLEVSRKLPAA+SVPYSS
Sbjct: 1261 SVLKRYKRRLSNKPVATLEVSRKLPAASSVPYSS 1294

BLAST of Carg25786 vs. NCBI nr
Match: XP_022963083.1 (ethylene-insensitive protein 2-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 2481.4 bits (6430), Expect = 0.0e+00
Identity = 1281/1292 (99.15%), Postives = 1285/1292 (99.46%), Query Frame = 0

Query: 42   MESMTSHTNHLSGTIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 101
            MESMTSHTNHLSG IHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN
Sbjct: 1    MESMTSHTNHLSGAIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 60

Query: 102  LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 161
            LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL
Sbjct: 61   LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 120

Query: 162  NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 221
            NLLLGWDLFTCVLLTGVVA LFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI
Sbjct: 121  NLLLGWDLFTCVLLTGVVAGLFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 180

Query: 222  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF 281
            PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIV QQQQSPPNISKEVLCNNHLF
Sbjct: 181  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIV-QQQQSPPNISKEVLCNNHLF 240

Query: 282  AIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL 341
            AIVCIFSGIY+VNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL
Sbjct: 241  AIVCIFSGIYLVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL 300

Query: 342  SNQITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF 401
            SN ITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF
Sbjct: 301  SNHITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF 360

Query: 402  SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG 461
            SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG
Sbjct: 361  SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG 420

Query: 462  NSDWVVNLRWNMGSGLSIPYVFFLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV 521
            NSDWVVNLRWNMGSGLSIPYV  LITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV
Sbjct: 421  NSDWVVNLRWNMGSGLSIPYVVLLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV 480

Query: 522  PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET 581
            PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET
Sbjct: 481  PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET 540

Query: 582  QSSSEVPSSPKYVSENLISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGD 641
            QSSSEVPSSPKYVSEN+ISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGD
Sbjct: 541  QSSSEVPSSPKYVSENVISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGD 600

Query: 642  LRSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGL 701
            L SEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGL
Sbjct: 601  LPSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGL 660

Query: 702  GRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSP 761
            GRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSP
Sbjct: 661  GRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSP 720

Query: 762  YSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSH 821
            YSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSH
Sbjct: 721  YSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSH 780

Query: 822  NGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKY 881
            NGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKY
Sbjct: 781  NGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKY 840

Query: 882  HTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPS 941
            HTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPS
Sbjct: 841  HTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPS 900

Query: 942  GTGENLSSVSNTKKYHSLPDIHRDQHISDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSR 1001
            GTGENLSSVSNTKKYHSLPDIHRDQH+SDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSR
Sbjct: 901  GTGENLSSVSNTKKYHSLPDIHRDQHVSDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSR 960

Query: 1002 SVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHT 1061
            SVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHT
Sbjct: 961  SVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHT 1020

Query: 1062 ISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEA 1121
            ISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEA
Sbjct: 1021 ISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEA 1080

Query: 1122 ETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIH 1181
            ETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIH
Sbjct: 1081 ETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIH 1140

Query: 1182 RILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQ 1241
            RILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQ
Sbjct: 1141 RILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQ 1200

Query: 1242 IANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASV 1301
            IANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASV
Sbjct: 1201 IANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASV 1260

Query: 1302 LKRYKRRLSNKPVATLEVSRKLPAAASVPYSS 1334
            LKRYKRRLSNKPVATLEVSRKLPAA+SVPYSS
Sbjct: 1261 LKRYKRRLSNKPVATLEVSRKLPAASSVPYSS 1291

BLAST of Carg25786 vs. ExPASy Swiss-Prot
Match: Q9S814 (Ethylene-insensitive protein 2 OS=Arabidopsis thaliana OX=3702 GN=EIN2 PE=1 SV=1)

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 690/1311 (52.63%), Postives = 881/1311 (67.20%), Query Frame = 0

Query: 42   MESMTSHTNHLSGTIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 101
            ME+   +     G I R +P + P LLVS+ Y+DPGKW A +EGGARFG+DL  + LLFN
Sbjct: 1    MEAEIVNVRPQLGFIQRMVPALLPVLLVSVGYIDPGKWVANIEGGARFGYDLVAITLLFN 60

Query: 102  LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 161
             AAILCQY++A I VVTG+ LAQIC+EEYDK TC+FLGIQAE S ILLDLTM++G++H L
Sbjct: 61   FAAILCQYVAARISVVTGKHLAQICNEEYDKWTCMFLGIQAEFSAILLDLTMVVGVAHAL 120

Query: 162  NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 221
            NLL G +L T V L  + A LFP+FA+ LE+G A  + I  AG +LL  V GVL+SQ EI
Sbjct: 121  NLLFGVELSTGVFLAAMDAFLFPVFASFLENGMANTVSIYSAGLVLLLYVSGVLLSQSEI 180

Query: 222  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF 281
            PLSMN +LTRLNGESAF LM LLGAS++PHNFY+HS    +   S  ++ K  LC +HLF
Sbjct: 181  PLSMNGVLTRLNGESAFALMGLLGASIVPHNFYIHSYFAGESTSS-SDVDKSSLCQDHLF 240

Query: 282  AIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL 341
            AI  +FSG+ +VN VLMN+AANVF+S+GL + TF DALSLMEQVF SP++ V+FL++LF 
Sbjct: 241  AIFGVFSGLSLVNYVLMNAAANVFHSTGLVVLTFHDALSLMEQVFMSPLIPVVFLMLLFF 300

Query: 342  SNQITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF 401
            S+QITAL W+ GG++VL +FLK++IP WLH ATIRI+A+ PALYCVW+SGA+G+YQLLIF
Sbjct: 301  SSQITALAWAFGGEVVLHDFLKIEIPAWLHRATIRILAVAPALYCVWTSGADGIYQLLIF 360

Query: 402  SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG 461
            +QV+VA++LP SVIPLFR+ASSR IMG  KI Q+ EF+A+  F+G LGL +VFVVEM FG
Sbjct: 361  TQVLVAMMLPCSVIPLFRIASSRQIMGVHKIPQVGEFLALTTFLGFLGLNVVFVVEMVFG 420

Query: 462  NSDWVVNLRWNMGSGLSIPYVFFLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV 521
            +SDW   LRWN   G SI Y   L+++C++ CL+LWLAATPLKSA+  A  +AQ+ N D 
Sbjct: 421  SSDWAGGLRWNTVMGTSIQYTTLLVSSCASLCLILWLAATPLKSASNRA--EAQIWNMDA 480

Query: 522  PEVKPDSAVEREGIDLGKGSYSAEPIASRSD---------LSVPEFDFDLPENIMEPVQV 581
                   +V+ E I+  +   + +    R +          SV    +DLPENI+   Q 
Sbjct: 481  QNALSYPSVQEEEIERTETRRNEDESIVRLESRVKDQLDTTSVTSSVYDLPENILMTDQE 540

Query: 582  LDSINQGETQ-----SSSEVPSSPKYVSENLISTELVSSSTVTHDVPDSTL-ADKKVLKI 641
            + S    E +     S+S+V S  +   ++ +  + V  STV ++V D  L  + K+ KI
Sbjct: 541  IRSSPPEERELDVKYSTSQVSSLKE---DSDVKEQSVLQSTVVNEVSDKDLIVETKMAKI 600

Query: 642  DSVEPVEKTVGFDGDLRSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSE 701
            + + PVEK V  + + +  +   E  +WE EE  K       +  S+GP SFRSLSG   
Sbjct: 601  EPMSPVEKIVSMENNSKFIEKDVEGVSWETEEATKAAPTSNFTVGSDGPPSFRSLSG--- 660

Query: 702  EGGNGTGSLSRLAGLGRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKL 761
            EGG+GTGSLSRL GLGRAARR L+AILDEFWG LYDFHG    +A+ KKLD L G D K 
Sbjct: 661  EGGSGTGSLSRLQGLGRAARRHLSAILDEFWGHLYDFHGQLVAEARAKKLDQLFGTDQK- 720

Query: 762  VTSSLKSDAVGKD--SPY-SSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQP 821
              SS+K+D+ GKD  S Y  SP      + ++S LYDS K  R    +DS YG+Q+G  P
Sbjct: 721  SASSMKADSFGKDISSGYCMSPTAKGMDSQMTSSLYDSLKQQRTPGSIDSLYGLQRGSSP 780

Query: 822  M-WSNHMQLFDQYVN-SSSHNGLDSGVKRYSSLRSLPSSESWDH-QPATVHGSQL-TYLS 881
                N MQ+   Y N ++++N  +   +RYSSLR+  SSE W+H QPATVHG Q+ +Y+ 
Sbjct: 781  SPLVNRMQMLGAYGNTTNNNNAYELSERRYSSLRAPSSSEGWEHQQPATVHGYQMKSYVD 840

Query: 882  RMAKDKSSG-HLNGQLDPSASKYHTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGF 941
             +AK++       G++  S S        G  S    +A A+ QK QNGL     PAPGF
Sbjct: 841  NLAKERLEALQSRGEIPTSRSM-----ALGTLSYTQQLALALKQKSQNGL--TPGPAPGF 900

Query: 942  PNFAVSRNPASESDRQYYDLSPSGTGENL-SSVSNTKKYHSLPDIH------RDQHI-SD 1001
             NFA SR+ + +S+R YY +  SG  + + ++V+N KKY S+PDI       R+ H+ ++
Sbjct: 901  ENFAGSRSISRQSERSYYGVPSSGNTDTVGAAVANEKKYSSMPDISGLSMSARNMHLPNN 960

Query: 1002 KSSQWDNVT---GYGSSISRIPVRGLSYSNSGSRSVAPLAFNELSSS-SVYGGALSPQMN 1061
            KS  WD  +   GYG+S  R+      YSN GSR   P  ++++S S   Y  A S   +
Sbjct: 961  KSGYWDPSSGGGGYGASYGRLSNESSLYSNLGSRVGVPSTYDDISQSRGGYRDAYSLPQS 1020

Query: 1062 PPLDSGSFWYRQPSEQFGLDKSSNS---ESKGIGRLHTISQEASFVVNSEARLLQSFRKC 1121
                +GS W RQP EQFG+ + + +   E +       I   AS  V++EA+LLQSFR C
Sbjct: 1021 ATTGTGSLWSRQPFEQFGVAERNGAVGEELRNRSNPINIDNNASSNVDAEAKLLQSFRHC 1080

Query: 1122 IVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEAETRVVRMKEPPPFSPDRRSGSG 1181
            I+KL+KLEGS+WLFGQSDG DEELID VAARE+F+YEAE R +                 
Sbjct: 1081 ILKLIKLEGSEWLFGQSDGVDEELIDRVAAREKFIYEAEAREINQV-------------- 1140

Query: 1182 MKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIHRILDLSLMESRPELWGKYTYVL 1241
                 +     +SSVP+CG+GC+WR+DLIVSFGVWCIHR+LDLSLMESRPELWGKYTYVL
Sbjct: 1141 ----GHMGEPLISSVPNCGDGCVWRADLIVSFGVWCIHRVLDLSLMESRPELWGKYTYVL 1200

Query: 1242 NRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQIANGMLPPAAKPGKGKSTTAAM 1301
            NRLQG+IDPAFSK R PM PCFCLQIP + Q  R+SP  ANGMLPPAAKP KGK TTA  
Sbjct: 1201 NRLQGVIDPAFSKLRTPMTPCFCLQIPASHQ--RASPTSANGMLPPAAKPAKGKCTTAVT 1260

Query: 1302 LLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASVLKRYKRRLSNKPV 1315
            LLD++KDVEMAIS RKGRTGTAAGDVAFPKGKENLASVLKRYKRRLSNKPV
Sbjct: 1261 LLDLIKDVEMAISCRKGRTGTAAGDVAFPKGKENLASVLKRYKRRLSNKPV 1274

BLAST of Carg25786 vs. ExPASy Swiss-Prot
Match: Q0D8I9 (Protein ETHYLENE-INSENSITIVE 2 OS=Oryza sativa subsp. japonica OX=39947 GN=EIN2 PE=2 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 2.4e-235
Identity = 545/1289 (42.28%), Postives = 761/1289 (59.04%), Query Frame = 0

Query: 63   IAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFNLAAILCQYLSASIGVVTGRGL 122
            + PALL+SI Y+D GKW A VE G+RFG DL +L LLFN  AILCQYL+A IG VTGR L
Sbjct: 31   LGPALLISIGYIDLGKWVAAVEAGSRFGLDLVLLALLFNFMAILCQYLAACIGTVTGRSL 90

Query: 123  AQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGLNLLLGW-DLFTCVLLTGVVAA 182
            A+IC +EY + TCIFLG+QA +S++  +LTMI GI+ G NLL  + DL T +    VV  
Sbjct: 91   AEICHQEYSRPTCIFLGVQAGLSLLTSELTMIFGIALGFNLLFEYDDLITGICFATVVPN 150

Query: 183  LFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEIPLSMNLMLTRLNGESAFTLM 242
            L P   + L       +  C+AGF LL  VLG+L+SQP+IPL+ N++  +L+GESA++LM
Sbjct: 151  LLPYAISHLGKKMVGTLNACIAGFALLCYVLGLLVSQPQIPLTTNVIFPKLSGESAYSLM 210

Query: 243  SLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLFAIVCIFSGIYVVNNVLMNSA 302
            +LLGA+VM HNFY+HSS+VQ Q++S    +   L ++HLF+++ IF+GI++VN+VLMNSA
Sbjct: 211  ALLGANVMAHNFYIHSSVVQGQKRSA--FAVGALFHDHLFSVLFIFTGIFLVNHVLMNSA 270

Query: 303  ANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFLSNQITALTWSLGGQLVLTNF 362
            A    S+   L TF D + LM Q+F +PM   +FL+VL  S+ I +LT ++G Q++  + 
Sbjct: 271  A--ADSTNTLLLTFQDVVELMNQIFVNPMAPTIFLVVLLFSSHIISLTSAIGSQVISQHL 330

Query: 363  LKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIFSQVMVALLLPSSVIPLFRVA 422
              +++P   H   ++  AI+PALYC   +GAEG+YQLLI  Q++ A+LLPSSV+PLFRVA
Sbjct: 331  FGINLPLSGHHLILKAFAIVPALYCAKVAGAEGIYQLLIICQIIQAMLLPSSVVPLFRVA 390

Query: 423  SSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFGNSDWVVNLRWNMGSGLSIPY 482
            SSR IMGA ++S  +E +  + F+ +L   I+F+ EM FG+S W+  L+ N GS +  P 
Sbjct: 391  SSRLIMGAHRVSLHLEILTFLAFLLMLFSNIIFMAEMLFGDSGWLNTLKGNTGSPVVFPS 450

Query: 483  VFFLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDVPEVKP--DSAVEREGIDLGK 542
               +  AC +    L++A TPLKS +  A+L  +   W VP  K   ++  +RE    G 
Sbjct: 451  TVLITVACVSVAFSLYMAVTPLKSGSHEAELQQE---WSVPSQKELLNTTQDREETCAGN 510

Query: 543  GSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSI---------NQGETQSSSEVPS- 602
             +Y  +    RSD+ VP       + +   +  +DS          +Q  T  +S  P  
Sbjct: 511  VTYEED---QRSDV-VPSPRIQPVDCLKSALDYIDSSDTAIESDHDSQHSTAHTSTAPES 570

Query: 603  --SPKYVSENLISTELVSSSTVTHDVPDSTLADKKVLK-IDSVEPVEKTVGFDGDLRSEK 662
              SP ++ E   S   V        + ++ +A++  ++ +DS    E+ +  +  L  + 
Sbjct: 571  CHSPSFIPEESKSVVAVDWPEPLEPISNAIVAEESTVESVDSKSTGERDIEVEPALLMDN 630

Query: 663  DSYEVDNWEAEEPLKEISGCLPSSTS-EGPGSFRSLSGKSEEGGNGTGSLSRLAGLGRAA 722
            D    +  E++   K + G  PS  S +GP S     GK  + GNG+GSLSRL+GLGRAA
Sbjct: 631  DKEAPNILESDN--KPLGGNNPSCASDDGPPSLTFSRGKGSDAGNGSGSLSRLSGLGRAA 690

Query: 723  RRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSPYSSP 782
            RRQL AILDEFWG L+D+HG  TQ+A  K+ D+LLG+D +  +S++++D+   + P S  
Sbjct: 691  RRQLAAILDEFWGHLFDYHGKLTQEASSKRFDILLGLDVRTPSSTVRADSQANEIPKSPM 750

Query: 783  LGSKASNPISSG-LYDSPKSLRVQSGLDSPYGVQKGH---QPMWSNHMQLFDQYVNSSSH 842
            +          G   D   +    S LD  YG+Q G+      WS  MQL    + SSS+
Sbjct: 751  VRDNLQGSAFLGSSRDLMSTKNEMSNLDLTYGLQMGNNIGSSAWSQGMQLPSTQLQSSSN 810

Query: 843  NGLDSGVKRYSSLRSLPS--SESWDHQPATVHGSQL-TYLSRMAKDK---SSGHLNGQLD 902
            + LD G  R +S  S PS    +  +QPAT+HG QL +YL +M  ++   SS  L+ Q  
Sbjct: 811  SLLDQGA-RLNSNFSTPSYADNNQFYQPATIHGYQLASYLKQMNANRNPYSSMPLDPQRL 870

Query: 903  PSASKYHTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQY 962
            P +S       +   +  DSV  A  Q L   LGA  +        A SR     ++R Y
Sbjct: 871  PKSS------ASAVPTYVDSVMHARNQNLLASLGATPSQIA-----ATSRIGTMMAERSY 930

Query: 963  YDLSPSGTGENLSSVSNTKKYHSLPDIH-----RDQHISDKSSQWDNVTGYGSSISRIPV 1022
            Y  S     EN  S + +KKYHS PDI          + ++S       G  S +SR+  
Sbjct: 931  YVPSTLDGNENAGSSAYSKKYHSSPDISALIAASRSALLNESKLGGGTIGSQSYLSRLAS 990

Query: 1023 RGLSYSNSGSRSVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQ-FGLDKS- 1082
                Y+NS +R  APLAF+ELS   + G   S Q +P   + S W +QP EQ FG+  + 
Sbjct: 991  ERSQYTNSVARPAAPLAFDELSPPKLPGDIFSMQQSPNPSARSLWAKQPFEQLFGVSSAE 1050

Query: 1083 -SNSESKGIGRLHTISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEEL 1142
             + SE    GR   ++++      SEA+LLQS R CI KLLKLEGS WLF Q+ G+DE+L
Sbjct: 1051 LTKSEFNPAGRSGGMTKDDFSYKESEAKLLQSLRFCISKLLKLEGSGWLFKQNGGSDEDL 1110

Query: 1143 IDCVAARERFLYEAET---RVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEG 1202
            ID VAA E+ L +  +    ++   + PP           K D  +  V    +P+CG+ 
Sbjct: 1111 IDQVAAVEKLLQQGTSDNQLLLGDTQQPPCD---------KADIQYMRV----LPNCGDD 1170

Query: 1203 CIWRSDLIVSFGVWCIHRILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPC 1262
            CIWR+ L+VSFGVWCI R+LDLSL+ESRPELWGKYTYVLNRLQGI+DPAFSKPR  +  C
Sbjct: 1171 CIWRASLVVSFGVWCIRRVLDLSLVESRPELWGKYTYVLNRLQGILDPAFSKPRSALSAC 1230

Query: 1263 FCLQIP-QAFQQKRSSPQIANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTG 1313
             CL    +     R S  +A   +P   +  +G  TTA+++L+M+KDVE A+S RKGR+G
Sbjct: 1231 ACLHRDIRVLNSLRHSSLVATNSIP---RQIRGSFTTASVVLEMIKDVETAVSGRKGRSG 1278

BLAST of Carg25786 vs. ExPASy Swiss-Prot
Match: Q553K4 (Natural resistance-associated macrophage protein 2 homolog OS=Dictyostelium discoideum OX=44689 GN=nramp2 PE=3 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 1.1e-51
Identity = 141/458 (30.79%), Postives = 246/458 (53.71%), Query Frame = 0

Query: 55  TIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFNLAAILCQYLSASI 114
           +I +   F+ PAL +S+ Y+DPG WA  +EGG+RFG+ L  ++L  N+ A+  Q L   +
Sbjct: 146 SIKKLKSFLGPALFISVGYMDPGNWATDLEGGSRFGYQLMWVLLFSNIMALFLQTLVIKL 205

Query: 115 GVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGLNLLLGWDLFTCVL 174
            +VT   LAQ C +EY K   IFL +  E+++I  DL  ++G + GLN+L G  L   V 
Sbjct: 206 ALVTKNDLAQQCRKEYSKTVNIFLWLILELAIISTDLAEVIGTAIGLNILFGLPLIAGVA 265

Query: 175 LTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEIPLSMNLMLTRLNG 234
           +T +   LF L        + + + + +   I +  V+ + +S+P      +  + RLN 
Sbjct: 266 ITSLDTLLF-LAIQRWGIRKLELLILLLLSMITMCFVIELFLSKPIASEVFSGFVPRLNS 325

Query: 235 ESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLFAI---VCIFSGIY 294
           +S      ++GA+ MPHN ++H S+V + ++ P +  K V+   + + +   V   +  +
Sbjct: 326 DSVMVATGIVGATTMPHNLFLHGSVV-KSRKIPNDRRKSVIKQAYRYNVIDTVLALNCAF 385

Query: 295 VVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFLSNQITALTWS 354
            VN  ++  AA+VF+ S + +    +A  L+ ++    +  VLF L LFL+ Q + +T +
Sbjct: 386 FVNIAILMLAASVFWKSNIQVTELSEAYRLLTKLMDGKLAAVLFGLGLFLAGQSSTITGT 445

Query: 355 LGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIFSQVMVALLLP 414
           + GQ+V+  F+KL I  WL     R++AIIPA   +   G +G Y LLI SQV++++ LP
Sbjct: 446 MAGQIVMEGFIKLRIKPWLRRFITRLLAIIPAAIVIIVLGDKGTYTLLIISQVLLSIGLP 505

Query: 415 SSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMF--FGNSDWVVNL 474
            +V+PL    SS  IMG FK    +  I  +I + I+GL +  + ++   F ++D +++ 
Sbjct: 506 FAVVPLIIFTSSYEIMGEFKNRLSIIIINSIIALFIIGLNLATIFQLINDFLHNDSIIS- 565

Query: 475 RWNMGSGLSIPYVFFLITACSAFCLMLWLAATPLKSAT 508
                    +  +F +  + +  CL+LWL  + +   T
Sbjct: 566 -------KCLTIIFLIPLSIALCCLLLWLIISKINFFT 593

BLAST of Carg25786 vs. ExPASy Swiss-Prot
Match: Q5HQ64 (Divalent metal cation transporter MntH OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=mntH PE=3 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 2.3e-44
Identity = 131/432 (30.32%), Postives = 224/432 (51.85%), Query Frame = 0

Query: 46  TSHTNHLSGTIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFNLAAI 105
           T + NH      +F+ F+ P LLV++ Y+DPG W  +++GGA++G+ L  ++L+ +L+A+
Sbjct: 25  TINFNHNDSASQKFLAFLGPGLLVAVGYMDPGNWITSMQGGAQYGYTLLFIILISSLSAM 84

Query: 106 LCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGLNLLL 165
           L Q ++  +G+ TG  LAQ+     +K   I   I AE+++I  D+  ++G +  L+L+ 
Sbjct: 85  LLQSMTVRLGIATGMDLAQMTRHFLNKPVAIMFWIIAELAIIATDIAEVIGSAIALDLIF 144

Query: 166 GWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEIPLSM 225
           G  L    L+T V      LF       + + +   +   +L   V  V IS P+I   +
Sbjct: 145 GIPLIVGALIT-VFDVFLLLFIMKFGFRKIEAIVGTLIFTVLAIFVFEVYISSPQITDML 204

Query: 226 NLMLTR----LNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF 285
           N  +       N    +  + ++GA++MPHN Y+HSSIVQ ++    +I ++     +  
Sbjct: 205 NGFVPHKEIITNQGILYIALGIIGATIMPHNLYLHSSIVQSRKYDRHSIHEKAQAIKYAT 264

Query: 286 AIVCI-FSGIYVVNNVLMNSAANV-----------FYSSGLALHTFPDALSLMEQVFRSP 345
               I  S  +VVN +L+   A +           FY   LAL T P   + +  +  + 
Sbjct: 265 IDSNIQLSIAFVVNCLLLTLGAALFFGTKTEDLGGFYDLYLALKTEPALGATLGGIMST- 324

Query: 346 MVYVLFLLVLFLSNQITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYC--V 405
               LF + L  S Q + +T +L GQ+V+  FLKL IP WL     R +A+IP + C  V
Sbjct: 325 ----LFAVALLASGQNSTITGTLAGQIVMEGFLKLSIPNWLRRLITRSLAVIPVIICLIV 384

Query: 406 WSSGAEGMYQLLIFSQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGI 460
           +    E + QLL+FSQV +++ LP S+IPL    S++ +MG FK    +  I+ ++ + +
Sbjct: 385 FKGNTEKIEQLLVFSQVFLSIALPFSLIPLQLATSNQNLMGPFKNKTWINIISWLLIIVL 444

BLAST of Carg25786 vs. ExPASy Swiss-Prot
Match: Q8CPM6 (Divalent metal cation transporter MntH OS=Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) OX=176280 GN=mntH PE=3 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 6.7e-44
Identity = 131/432 (30.32%), Postives = 223/432 (51.62%), Query Frame = 0

Query: 46  TSHTNHLSGTIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFNLAAI 105
           T + NH      +F+ F+ P LLV++ Y+DPG W  +++GGA++G+ L  ++L+ +L+A+
Sbjct: 25  TINFNHNDSASQKFLAFLGPGLLVAVGYMDPGNWITSMQGGAQYGYTLLFIILISSLSAM 84

Query: 106 LCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGLNLLL 165
           L Q ++  +G+ TG  LAQ+     +K   I   I AE+++I  D+  ++G +  L+L+ 
Sbjct: 85  LLQSMTVRLGIATGMDLAQMTRHFLNKPVAIMFWIIAELAIIATDIAEVIGSAIALDLIF 144

Query: 166 GWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEIPLSM 225
           G  L    L+T V      LF       + + +   +   +L   V  V IS P I   +
Sbjct: 145 GIPLIVGALIT-VFDVFLLLFIMKFGFRKIEAIVGTLIFTVLAIFVFEVYISSPHIIDML 204

Query: 226 NLMLTR----LNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF 285
           N  +       N    +  + ++GA++MPHN Y+HSSIVQ ++    +I ++     +  
Sbjct: 205 NGFVPHKEIITNQGILYIALGIIGATIMPHNLYLHSSIVQSRKYDRHSIHEKAQAIKYAT 264

Query: 286 AIVCI-FSGIYVVNNVLMNSAANV-----------FYSSGLALHTFPDALSLMEQVFRSP 345
               I  S  +VVN +L+   A +           FY   LAL T P   + +  +  + 
Sbjct: 265 IDSNIQLSIAFVVNCLLLTLGAALFFGTKTEDLGGFYDLYLALKTEPALGATLGGIMST- 324

Query: 346 MVYVLFLLVLFLSNQITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYC--V 405
               LF + L  S Q + +T +L GQ+V+  FLKL IP WL     R +A+IP + C  V
Sbjct: 325 ----LFAVALLASGQNSTITGTLAGQIVMEGFLKLSIPNWLRRLITRSLAVIPVIICLIV 384

Query: 406 WSSGAEGMYQLLIFSQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGI 460
           +    E + QLL+FSQV +++ LP S+IPL    S++ +MG FK    +  I+ ++ + +
Sbjct: 385 FKGNTEKIEQLLVFSQVFLSIALPFSLIPLQLATSNQNLMGPFKNKTWINIISWLLIIVL 444

BLAST of Carg25786 vs. ExPASy TrEMBL
Match: A0A6J1HE97 (ethylene-insensitive protein 2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111463387 PE=3 SV=1)

HSP 1 Score: 2488.0 bits (6447), Expect = 0.0e+00
Identity = 1282/1292 (99.23%), Postives = 1286/1292 (99.54%), Query Frame = 0

Query: 42   MESMTSHTNHLSGTIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 101
            MESMTSHTNHLSG IHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN
Sbjct: 1    MESMTSHTNHLSGAIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 60

Query: 102  LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 161
            LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL
Sbjct: 61   LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 120

Query: 162  NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 221
            NLLLGWDLFTCVLLTGVVA LFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI
Sbjct: 121  NLLLGWDLFTCVLLTGVVAGLFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 180

Query: 222  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF 281
            PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF
Sbjct: 181  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF 240

Query: 282  AIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL 341
            AIVCIFSGIY+VNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL
Sbjct: 241  AIVCIFSGIYLVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL 300

Query: 342  SNQITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF 401
            SN ITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF
Sbjct: 301  SNHITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF 360

Query: 402  SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG 461
            SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG
Sbjct: 361  SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG 420

Query: 462  NSDWVVNLRWNMGSGLSIPYVFFLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV 521
            NSDWVVNLRWNMGSGLSIPYV  LITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV
Sbjct: 421  NSDWVVNLRWNMGSGLSIPYVVLLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV 480

Query: 522  PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET 581
            PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET
Sbjct: 481  PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET 540

Query: 582  QSSSEVPSSPKYVSENLISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGD 641
            QSSSEVPSSPKYVSEN+ISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGD
Sbjct: 541  QSSSEVPSSPKYVSENVISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGD 600

Query: 642  LRSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGL 701
            L SEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGL
Sbjct: 601  LPSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGL 660

Query: 702  GRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSP 761
            GRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSP
Sbjct: 661  GRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSP 720

Query: 762  YSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSH 821
            YSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSH
Sbjct: 721  YSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSH 780

Query: 822  NGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKY 881
            NGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKY
Sbjct: 781  NGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKY 840

Query: 882  HTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPS 941
            HTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPS
Sbjct: 841  HTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPS 900

Query: 942  GTGENLSSVSNTKKYHSLPDIHRDQHISDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSR 1001
            GTGENLSSVSNTKKYHSLPDIHRDQH+SDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSR
Sbjct: 901  GTGENLSSVSNTKKYHSLPDIHRDQHVSDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSR 960

Query: 1002 SVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHT 1061
            SVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHT
Sbjct: 961  SVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHT 1020

Query: 1062 ISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEA 1121
            ISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEA
Sbjct: 1021 ISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEA 1080

Query: 1122 ETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIH 1181
            ETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIH
Sbjct: 1081 ETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIH 1140

Query: 1182 RILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQ 1241
            RILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQ
Sbjct: 1141 RILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQ 1200

Query: 1242 IANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASV 1301
            IANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASV
Sbjct: 1201 IANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASV 1260

Query: 1302 LKRYKRRLSNKPVATLEVSRKLPAAASVPYSS 1334
            LKRYKRRLSNKPVATLEVSRKLPAA+SVPYSS
Sbjct: 1261 LKRYKRRLSNKPVATLEVSRKLPAASSVPYSS 1292

BLAST of Carg25786 vs. ExPASy TrEMBL
Match: A0A6J1HF34 (ethylene-insensitive protein 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111463387 PE=3 SV=1)

HSP 1 Score: 2483.0 bits (6434), Expect = 0.0e+00
Identity = 1282/1294 (99.07%), Postives = 1286/1294 (99.38%), Query Frame = 0

Query: 42   MESMTSHTNHLSGTIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 101
            MESMTSHTNHLSG IHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN
Sbjct: 1    MESMTSHTNHLSGAIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 60

Query: 102  LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 161
            LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL
Sbjct: 61   LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 120

Query: 162  NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 221
            NLLLGWDLFTCVLLTGVVA LFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI
Sbjct: 121  NLLLGWDLFTCVLLTGVVAGLFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 180

Query: 222  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIV--QQQQQSPPNISKEVLCNNH 281
            PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIV  QQQQQSPPNISKEVLCNNH
Sbjct: 181  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQQQSPPNISKEVLCNNH 240

Query: 282  LFAIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVL 341
            LFAIVCIFSGIY+VNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVL
Sbjct: 241  LFAIVCIFSGIYLVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVL 300

Query: 342  FLSNQITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLL 401
            FLSN ITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLL
Sbjct: 301  FLSNHITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLL 360

Query: 402  IFSQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMF 461
            IFSQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMF
Sbjct: 361  IFSQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMF 420

Query: 462  FGNSDWVVNLRWNMGSGLSIPYVFFLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNW 521
            FGNSDWVVNLRWNMGSGLSIPYV  LITACSAFCLMLWLAATPLKSATAIAQLDAQVLNW
Sbjct: 421  FGNSDWVVNLRWNMGSGLSIPYVVLLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNW 480

Query: 522  DVPEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQG 581
            DVPEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQG
Sbjct: 481  DVPEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQG 540

Query: 582  ETQSSSEVPSSPKYVSENLISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFD 641
            ETQSSSEVPSSPKYVSEN+ISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFD
Sbjct: 541  ETQSSSEVPSSPKYVSENVISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFD 600

Query: 642  GDLRSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLA 701
            GDL SEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLA
Sbjct: 601  GDLPSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLA 660

Query: 702  GLGRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKD 761
            GLGRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKD
Sbjct: 661  GLGRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKD 720

Query: 762  SPYSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSS 821
            SPYSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSS
Sbjct: 721  SPYSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSS 780

Query: 822  SHNGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSAS 881
            SHNGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSAS
Sbjct: 781  SHNGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSAS 840

Query: 882  KYHTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLS 941
            KYHTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLS
Sbjct: 841  KYHTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLS 900

Query: 942  PSGTGENLSSVSNTKKYHSLPDIHRDQHISDKSSQWDNVTGYGSSISRIPVRGLSYSNSG 1001
            PSGTGENLSSVSNTKKYHSLPDIHRDQH+SDKSSQWDNVTGYGSSISRIPVRGLSYSNSG
Sbjct: 901  PSGTGENLSSVSNTKKYHSLPDIHRDQHVSDKSSQWDNVTGYGSSISRIPVRGLSYSNSG 960

Query: 1002 SRSVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRL 1061
            SRSVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRL
Sbjct: 961  SRSVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRL 1020

Query: 1062 HTISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLY 1121
            HTISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLY
Sbjct: 1021 HTISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLY 1080

Query: 1122 EAETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWC 1181
            EAETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWC
Sbjct: 1081 EAETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWC 1140

Query: 1182 IHRILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSS 1241
            IHRILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSS
Sbjct: 1141 IHRILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSS 1200

Query: 1242 PQIANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLA 1301
            PQIANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLA
Sbjct: 1201 PQIANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLA 1260

Query: 1302 SVLKRYKRRLSNKPVATLEVSRKLPAAASVPYSS 1334
            SVLKRYKRRLSNKPVATLEVSRKLPAA+SVPYSS
Sbjct: 1261 SVLKRYKRRLSNKPVATLEVSRKLPAASSVPYSS 1294

BLAST of Carg25786 vs. ExPASy TrEMBL
Match: A0A6J1HGQ2 (ethylene-insensitive protein 2-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111463387 PE=3 SV=1)

HSP 1 Score: 2481.4 bits (6430), Expect = 0.0e+00
Identity = 1281/1292 (99.15%), Postives = 1285/1292 (99.46%), Query Frame = 0

Query: 42   MESMTSHTNHLSGTIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 101
            MESMTSHTNHLSG IHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN
Sbjct: 1    MESMTSHTNHLSGAIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 60

Query: 102  LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 161
            LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL
Sbjct: 61   LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 120

Query: 162  NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 221
            NLLLGWDLFTCVLLTGVVA LFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI
Sbjct: 121  NLLLGWDLFTCVLLTGVVAGLFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 180

Query: 222  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF 281
            PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIV QQQQSPPNISKEVLCNNHLF
Sbjct: 181  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIV-QQQQSPPNISKEVLCNNHLF 240

Query: 282  AIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL 341
            AIVCIFSGIY+VNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL
Sbjct: 241  AIVCIFSGIYLVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL 300

Query: 342  SNQITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF 401
            SN ITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF
Sbjct: 301  SNHITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF 360

Query: 402  SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG 461
            SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG
Sbjct: 361  SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG 420

Query: 462  NSDWVVNLRWNMGSGLSIPYVFFLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV 521
            NSDWVVNLRWNMGSGLSIPYV  LITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV
Sbjct: 421  NSDWVVNLRWNMGSGLSIPYVVLLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV 480

Query: 522  PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET 581
            PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET
Sbjct: 481  PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET 540

Query: 582  QSSSEVPSSPKYVSENLISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGD 641
            QSSSEVPSSPKYVSEN+ISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGD
Sbjct: 541  QSSSEVPSSPKYVSENVISTELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDGD 600

Query: 642  LRSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGL 701
            L SEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGL
Sbjct: 601  LPSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAGL 660

Query: 702  GRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSP 761
            GRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSP
Sbjct: 661  GRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDSP 720

Query: 762  YSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSH 821
            YSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSH
Sbjct: 721  YSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSSH 780

Query: 822  NGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKY 881
            NGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKY
Sbjct: 781  NGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASKY 840

Query: 882  HTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPS 941
            HTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPS
Sbjct: 841  HTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSPS 900

Query: 942  GTGENLSSVSNTKKYHSLPDIHRDQHISDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSR 1001
            GTGENLSSVSNTKKYHSLPDIHRDQH+SDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSR
Sbjct: 901  GTGENLSSVSNTKKYHSLPDIHRDQHVSDKSSQWDNVTGYGSSISRIPVRGLSYSNSGSR 960

Query: 1002 SVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHT 1061
            SVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHT
Sbjct: 961  SVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLHT 1020

Query: 1062 ISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEA 1121
            ISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEA
Sbjct: 1021 ISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEA 1080

Query: 1122 ETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIH 1181
            ETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIH
Sbjct: 1081 ETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIH 1140

Query: 1182 RILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQ 1241
            RILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQ
Sbjct: 1141 RILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQ 1200

Query: 1242 IANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASV 1301
            IANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASV
Sbjct: 1201 IANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASV 1260

Query: 1302 LKRYKRRLSNKPVATLEVSRKLPAAASVPYSS 1334
            LKRYKRRLSNKPVATLEVSRKLPAA+SVPYSS
Sbjct: 1261 LKRYKRRLSNKPVATLEVSRKLPAASSVPYSS 1291

BLAST of Carg25786 vs. ExPASy TrEMBL
Match: A0A6J1IAF9 (ethylene-insensitive protein 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471133 PE=3 SV=1)

HSP 1 Score: 2444.8 bits (6335), Expect = 0.0e+00
Identity = 1264/1293 (97.76%), Postives = 1273/1293 (98.45%), Query Frame = 0

Query: 42   MESMTSHTNHLSGTIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 101
            MES TSHTNHLSG IHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN
Sbjct: 1    MESTTSHTNHLSGAIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 60

Query: 102  LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 161
            LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL
Sbjct: 61   LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 120

Query: 162  NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 221
            NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI
Sbjct: 121  NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 180

Query: 222  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF 281
            PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF
Sbjct: 181  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF 240

Query: 282  AIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL 341
            AIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL
Sbjct: 241  AIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL 300

Query: 342  SNQITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF 401
            SNQITA TWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF
Sbjct: 301  SNQITAFTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF 360

Query: 402  SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG 461
            SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG
Sbjct: 361  SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG 420

Query: 462  NSDWVVNLRWNMGSGLSIPYVFFLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV 521
            NSDWVVNLRWNMGSGLSIPYV  LITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV
Sbjct: 421  NSDWVVNLRWNMGSGLSIPYVVLLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV 480

Query: 522  PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET 581
            PEVKPDSAVEREGIDLGKGSYSAEPIAS SDLSVPEFDFDLPENIMEPVQVLDSINQGET
Sbjct: 481  PEVKPDSAVEREGIDLGKGSYSAEPIASHSDLSVPEFDFDLPENIMEPVQVLDSINQGET 540

Query: 582  QSSSEVPSSPKYVSENLIST-ELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDG 641
            QSSS VPSSPKYVSENLIST ELVSSSTVT D PDSTLADKKVLKIDSVEPVEKTVG DG
Sbjct: 541  QSSSVVPSSPKYVSENLISTEELVSSSTVTRDDPDSTLADKKVLKIDSVEPVEKTVGLDG 600

Query: 642  DLRSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAG 701
            DLRSEKDSYEVDNWEAEEPLKEISGCLPSS SEGPGSFRSLSGKSEEGGNGTGSLSRLAG
Sbjct: 601  DLRSEKDSYEVDNWEAEEPLKEISGCLPSSASEGPGSFRSLSGKSEEGGNGTGSLSRLAG 660

Query: 702  LGRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDS 761
            LGRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDS
Sbjct: 661  LGRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDS 720

Query: 762  PYSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSS 821
            PYSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVN+SS
Sbjct: 721  PYSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNNSS 780

Query: 822  HNGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASK 881
            HNGLDSGVKRYSSLRSLPSSESWDHQPATV GSQL+YLSRMAKD+SSGHLNGQLDPSASK
Sbjct: 781  HNGLDSGVKRYSSLRSLPSSESWDHQPATVDGSQLSYLSRMAKDRSSGHLNGQLDPSASK 840

Query: 882  YHTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSP 941
            YHTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSP
Sbjct: 841  YHTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSP 900

Query: 942  SGTGENLSSVSNTKKYHSLPDIHRDQHISDKSSQWDNVTGYGSSISRIPVRGLSYSNSGS 1001
            SGTGENLSSVSNTKKYHSLPDIHRDQH+SDKSSQWDNVTGYGSSISRIP RGLSY+NSGS
Sbjct: 901  SGTGENLSSVSNTKKYHSLPDIHRDQHVSDKSSQWDNVTGYGSSISRIPSRGLSYTNSGS 960

Query: 1002 RSVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLH 1061
            RSVAPLAFNELSSS VYGGALSPQMNPPLDSGSFWYRQPSEQFGL K+SNSESKGIGRLH
Sbjct: 961  RSVAPLAFNELSSSGVYGGALSPQMNPPLDSGSFWYRQPSEQFGLGKNSNSESKGIGRLH 1020

Query: 1062 TISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYE 1121
            TISQEASFVVNSEARLLQSFR CIV+LLKLEGSDWLFGQSDGADEELIDCVAARE+FLYE
Sbjct: 1021 TISQEASFVVNSEARLLQSFRDCIVRLLKLEGSDWLFGQSDGADEELIDCVAAREKFLYE 1080

Query: 1122 AETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCI 1181
            AETRVVRMKEPP FSPDRRSGSG+KNDSNFANVS SSVPHCGEGCIWRSDLIVSFGVWCI
Sbjct: 1081 AETRVVRMKEPPSFSPDRRSGSGLKNDSNFANVSKSSVPHCGEGCIWRSDLIVSFGVWCI 1140

Query: 1182 HRILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSP 1241
            HRILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSP
Sbjct: 1141 HRILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSP 1200

Query: 1242 QIANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLAS 1301
            QIANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLAS
Sbjct: 1201 QIANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLAS 1260

Query: 1302 VLKRYKRRLSNKPVATLEVSRKLPAAASVPYSS 1334
            VLKRYKRRL NKPVATLEVSRKLPAAASVPYSS
Sbjct: 1261 VLKRYKRRLFNKPVATLEVSRKLPAAASVPYSS 1293

BLAST of Carg25786 vs. ExPASy TrEMBL
Match: A0A6J1IC11 (ethylene-insensitive protein 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111471133 PE=3 SV=1)

HSP 1 Score: 2438.3 bits (6318), Expect = 0.0e+00
Identity = 1263/1293 (97.68%), Postives = 1272/1293 (98.38%), Query Frame = 0

Query: 42   MESMTSHTNHLSGTIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 101
            MES TSHTNHLSG IHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN
Sbjct: 1    MESTTSHTNHLSGAIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 60

Query: 102  LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 161
            LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL
Sbjct: 61   LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 120

Query: 162  NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 221
            NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI
Sbjct: 121  NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 180

Query: 222  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF 281
            PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIV QQQQSPPNISKEVLCNNHLF
Sbjct: 181  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIV-QQQQSPPNISKEVLCNNHLF 240

Query: 282  AIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL 341
            AIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL
Sbjct: 241  AIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL 300

Query: 342  SNQITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF 401
            SNQITA TWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF
Sbjct: 301  SNQITAFTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF 360

Query: 402  SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG 461
            SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG
Sbjct: 361  SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG 420

Query: 462  NSDWVVNLRWNMGSGLSIPYVFFLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV 521
            NSDWVVNLRWNMGSGLSIPYV  LITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV
Sbjct: 421  NSDWVVNLRWNMGSGLSIPYVVLLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV 480

Query: 522  PEVKPDSAVEREGIDLGKGSYSAEPIASRSDLSVPEFDFDLPENIMEPVQVLDSINQGET 581
            PEVKPDSAVEREGIDLGKGSYSAEPIAS SDLSVPEFDFDLPENIMEPVQVLDSINQGET
Sbjct: 481  PEVKPDSAVEREGIDLGKGSYSAEPIASHSDLSVPEFDFDLPENIMEPVQVLDSINQGET 540

Query: 582  QSSSEVPSSPKYVSENLIST-ELVSSSTVTHDVPDSTLADKKVLKIDSVEPVEKTVGFDG 641
            QSSS VPSSPKYVSENLIST ELVSSSTVT D PDSTLADKKVLKIDSVEPVEKTVG DG
Sbjct: 541  QSSSVVPSSPKYVSENLISTEELVSSSTVTRDDPDSTLADKKVLKIDSVEPVEKTVGLDG 600

Query: 642  DLRSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSEEGGNGTGSLSRLAG 701
            DLRSEKDSYEVDNWEAEEPLKEISGCLPSS SEGPGSFRSLSGKSEEGGNGTGSLSRLAG
Sbjct: 601  DLRSEKDSYEVDNWEAEEPLKEISGCLPSSASEGPGSFRSLSGKSEEGGNGTGSLSRLAG 660

Query: 702  LGRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDS 761
            LGRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDS
Sbjct: 661  LGRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKLVTSSLKSDAVGKDS 720

Query: 762  PYSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNSSS 821
            PYSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVN+SS
Sbjct: 721  PYSSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQPMWSNHMQLFDQYVNNSS 780

Query: 822  HNGLDSGVKRYSSLRSLPSSESWDHQPATVHGSQLTYLSRMAKDKSSGHLNGQLDPSASK 881
            HNGLDSGVKRYSSLRSLPSSESWDHQPATV GSQL+YLSRMAKD+SSGHLNGQLDPSASK
Sbjct: 781  HNGLDSGVKRYSSLRSLPSSESWDHQPATVDGSQLSYLSRMAKDRSSGHLNGQLDPSASK 840

Query: 882  YHTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSP 941
            YHTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSP
Sbjct: 841  YHTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGFPNFAVSRNPASESDRQYYDLSP 900

Query: 942  SGTGENLSSVSNTKKYHSLPDIHRDQHISDKSSQWDNVTGYGSSISRIPVRGLSYSNSGS 1001
            SGTGENLSSVSNTKKYHSLPDIHRDQH+SDKSSQWDNVTGYGSSISRIP RGLSY+NSGS
Sbjct: 901  SGTGENLSSVSNTKKYHSLPDIHRDQHVSDKSSQWDNVTGYGSSISRIPSRGLSYTNSGS 960

Query: 1002 RSVAPLAFNELSSSSVYGGALSPQMNPPLDSGSFWYRQPSEQFGLDKSSNSESKGIGRLH 1061
            RSVAPLAFNELSSS VYGGALSPQMNPPLDSGSFWYRQPSEQFGL K+SNSESKGIGRLH
Sbjct: 961  RSVAPLAFNELSSSGVYGGALSPQMNPPLDSGSFWYRQPSEQFGLGKNSNSESKGIGRLH 1020

Query: 1062 TISQEASFVVNSEARLLQSFRKCIVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYE 1121
            TISQEASFVVNSEARLLQSFR CIV+LLKLEGSDWLFGQSDGADEELIDCVAARE+FLYE
Sbjct: 1021 TISQEASFVVNSEARLLQSFRDCIVRLLKLEGSDWLFGQSDGADEELIDCVAAREKFLYE 1080

Query: 1122 AETRVVRMKEPPPFSPDRRSGSGMKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCI 1181
            AETRVVRMKEPP FSPDRRSGSG+KNDSNFANVS SSVPHCGEGCIWRSDLIVSFGVWCI
Sbjct: 1081 AETRVVRMKEPPSFSPDRRSGSGLKNDSNFANVSKSSVPHCGEGCIWRSDLIVSFGVWCI 1140

Query: 1182 HRILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSP 1241
            HRILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSP
Sbjct: 1141 HRILDLSLMESRPELWGKYTYVLNRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSP 1200

Query: 1242 QIANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLAS 1301
            QIANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLAS
Sbjct: 1201 QIANGMLPPAAKPGKGKSTTAAMLLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLAS 1260

Query: 1302 VLKRYKRRLSNKPVATLEVSRKLPAAASVPYSS 1334
            VLKRYKRRL NKPVATLEVSRKLPAAASVPYSS
Sbjct: 1261 VLKRYKRRLFNKPVATLEVSRKLPAAASVPYSS 1292

BLAST of Carg25786 vs. TAIR 10
Match: AT5G03280.1 (NRAMP metal ion transporter family protein )

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 690/1311 (52.63%), Postives = 881/1311 (67.20%), Query Frame = 0

Query: 42   MESMTSHTNHLSGTIHRFMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFN 101
            ME+   +     G I R +P + P LLVS+ Y+DPGKW A +EGGARFG+DL  + LLFN
Sbjct: 1    MEAEIVNVRPQLGFIQRMVPALLPVLLVSVGYIDPGKWVANIEGGARFGYDLVAITLLFN 60

Query: 102  LAAILCQYLSASIGVVTGRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGL 161
             AAILCQY++A I VVTG+ LAQIC+EEYDK TC+FLGIQAE S ILLDLTM++G++H L
Sbjct: 61   FAAILCQYVAARISVVTGKHLAQICNEEYDKWTCMFLGIQAEFSAILLDLTMVVGVAHAL 120

Query: 162  NLLLGWDLFTCVLLTGVVAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI 221
            NLL G +L T V L  + A LFP+FA+ LE+G A  + I  AG +LL  V GVL+SQ EI
Sbjct: 121  NLLFGVELSTGVFLAAMDAFLFPVFASFLENGMANTVSIYSAGLVLLLYVSGVLLSQSEI 180

Query: 222  PLSMNLMLTRLNGESAFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLF 281
            PLSMN +LTRLNGESAF LM LLGAS++PHNFY+HS    +   S  ++ K  LC +HLF
Sbjct: 181  PLSMNGVLTRLNGESAFALMGLLGASIVPHNFYIHSYFAGESTSS-SDVDKSSLCQDHLF 240

Query: 282  AIVCIFSGIYVVNNVLMNSAANVFYSSGLALHTFPDALSLMEQVFRSPMVYVLFLLVLFL 341
            AI  +FSG+ +VN VLMN+AANVF+S+GL + TF DALSLMEQVF SP++ V+FL++LF 
Sbjct: 241  AIFGVFSGLSLVNYVLMNAAANVFHSTGLVVLTFHDALSLMEQVFMSPLIPVVFLMLLFF 300

Query: 342  SNQITALTWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIF 401
            S+QITAL W+ GG++VL +FLK++IP WLH ATIRI+A+ PALYCVW+SGA+G+YQLLIF
Sbjct: 301  SSQITALAWAFGGEVVLHDFLKIEIPAWLHRATIRILAVAPALYCVWTSGADGIYQLLIF 360

Query: 402  SQVMVALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFG 461
            +QV+VA++LP SVIPLFR+ASSR IMG  KI Q+ EF+A+  F+G LGL +VFVVEM FG
Sbjct: 361  TQVLVAMMLPCSVIPLFRIASSRQIMGVHKIPQVGEFLALTTFLGFLGLNVVFVVEMVFG 420

Query: 462  NSDWVVNLRWNMGSGLSIPYVFFLITACSAFCLMLWLAATPLKSATAIAQLDAQVLNWDV 521
            +SDW   LRWN   G SI Y   L+++C++ CL+LWLAATPLKSA+  A  +AQ+ N D 
Sbjct: 421  SSDWAGGLRWNTVMGTSIQYTTLLVSSCASLCLILWLAATPLKSASNRA--EAQIWNMDA 480

Query: 522  PEVKPDSAVEREGIDLGKGSYSAEPIASRSD---------LSVPEFDFDLPENIMEPVQV 581
                   +V+ E I+  +   + +    R +          SV    +DLPENI+   Q 
Sbjct: 481  QNALSYPSVQEEEIERTETRRNEDESIVRLESRVKDQLDTTSVTSSVYDLPENILMTDQE 540

Query: 582  LDSINQGETQ-----SSSEVPSSPKYVSENLISTELVSSSTVTHDVPDSTL-ADKKVLKI 641
            + S    E +     S+S+V S  +   ++ +  + V  STV ++V D  L  + K+ KI
Sbjct: 541  IRSSPPEERELDVKYSTSQVSSLKE---DSDVKEQSVLQSTVVNEVSDKDLIVETKMAKI 600

Query: 642  DSVEPVEKTVGFDGDLRSEKDSYEVDNWEAEEPLKEISGCLPSSTSEGPGSFRSLSGKSE 701
            + + PVEK V  + + +  +   E  +WE EE  K       +  S+GP SFRSLSG   
Sbjct: 601  EPMSPVEKIVSMENNSKFIEKDVEGVSWETEEATKAAPTSNFTVGSDGPPSFRSLSG--- 660

Query: 702  EGGNGTGSLSRLAGLGRAARRQLTAILDEFWGQLYDFHGVATQDAKDKKLDLLLGIDSKL 761
            EGG+GTGSLSRL GLGRAARR L+AILDEFWG LYDFHG    +A+ KKLD L G D K 
Sbjct: 661  EGGSGTGSLSRLQGLGRAARRHLSAILDEFWGHLYDFHGQLVAEARAKKLDQLFGTDQK- 720

Query: 762  VTSSLKSDAVGKD--SPY-SSPLGSKASNPISSGLYDSPKSLRVQSGLDSPYGVQKGHQP 821
              SS+K+D+ GKD  S Y  SP      + ++S LYDS K  R    +DS YG+Q+G  P
Sbjct: 721  SASSMKADSFGKDISSGYCMSPTAKGMDSQMTSSLYDSLKQQRTPGSIDSLYGLQRGSSP 780

Query: 822  M-WSNHMQLFDQYVN-SSSHNGLDSGVKRYSSLRSLPSSESWDH-QPATVHGSQL-TYLS 881
                N MQ+   Y N ++++N  +   +RYSSLR+  SSE W+H QPATVHG Q+ +Y+ 
Sbjct: 781  SPLVNRMQMLGAYGNTTNNNNAYELSERRYSSLRAPSSSEGWEHQQPATVHGYQMKSYVD 840

Query: 882  RMAKDKSSG-HLNGQLDPSASKYHTLGGAGGASLRDSVAFAMGQKLQNGLGACQAPAPGF 941
             +AK++       G++  S S        G  S    +A A+ QK QNGL     PAPGF
Sbjct: 841  NLAKERLEALQSRGEIPTSRSM-----ALGTLSYTQQLALALKQKSQNGL--TPGPAPGF 900

Query: 942  PNFAVSRNPASESDRQYYDLSPSGTGENL-SSVSNTKKYHSLPDIH------RDQHI-SD 1001
             NFA SR+ + +S+R YY +  SG  + + ++V+N KKY S+PDI       R+ H+ ++
Sbjct: 901  ENFAGSRSISRQSERSYYGVPSSGNTDTVGAAVANEKKYSSMPDISGLSMSARNMHLPNN 960

Query: 1002 KSSQWDNVT---GYGSSISRIPVRGLSYSNSGSRSVAPLAFNELSSS-SVYGGALSPQMN 1061
            KS  WD  +   GYG+S  R+      YSN GSR   P  ++++S S   Y  A S   +
Sbjct: 961  KSGYWDPSSGGGGYGASYGRLSNESSLYSNLGSRVGVPSTYDDISQSRGGYRDAYSLPQS 1020

Query: 1062 PPLDSGSFWYRQPSEQFGLDKSSNS---ESKGIGRLHTISQEASFVVNSEARLLQSFRKC 1121
                +GS W RQP EQFG+ + + +   E +       I   AS  V++EA+LLQSFR C
Sbjct: 1021 ATTGTGSLWSRQPFEQFGVAERNGAVGEELRNRSNPINIDNNASSNVDAEAKLLQSFRHC 1080

Query: 1122 IVKLLKLEGSDWLFGQSDGADEELIDCVAARERFLYEAETRVVRMKEPPPFSPDRRSGSG 1181
            I+KL+KLEGS+WLFGQSDG DEELID VAARE+F+YEAE R +                 
Sbjct: 1081 ILKLIKLEGSEWLFGQSDGVDEELIDRVAAREKFIYEAEAREINQV-------------- 1140

Query: 1182 MKNDSNFANVSVSSVPHCGEGCIWRSDLIVSFGVWCIHRILDLSLMESRPELWGKYTYVL 1241
                 +     +SSVP+CG+GC+WR+DLIVSFGVWCIHR+LDLSLMESRPELWGKYTYVL
Sbjct: 1141 ----GHMGEPLISSVPNCGDGCVWRADLIVSFGVWCIHRVLDLSLMESRPELWGKYTYVL 1200

Query: 1242 NRLQGIIDPAFSKPRIPMPPCFCLQIPQAFQQKRSSPQIANGMLPPAAKPGKGKSTTAAM 1301
            NRLQG+IDPAFSK R PM PCFCLQIP + Q  R+SP  ANGMLPPAAKP KGK TTA  
Sbjct: 1201 NRLQGVIDPAFSKLRTPMTPCFCLQIPASHQ--RASPTSANGMLPPAAKPAKGKCTTAVT 1260

Query: 1302 LLDMVKDVEMAISSRKGRTGTAAGDVAFPKGKENLASVLKRYKRRLSNKPV 1315
            LLD++KDVEMAIS RKGRTGTAAGDVAFPKGKENLASVLKRYKRRLSNKPV
Sbjct: 1261 LLDLIKDVEMAISCRKGRTGTAAGDVAFPKGKENLASVLKRYKRRLSNKPV 1274

BLAST of Carg25786 vs. TAIR 10
Match: AT1G15960.1 (NRAMP metal ion transporter 6 )

HSP 1 Score: 150.6 bits (379), Expect = 8.9e-36
Identity = 118/410 (28.78%), Postives = 213/410 (51.95%), Query Frame = 0

Query: 59  FMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFNLAAILCQYLSASIGVVT 118
           F  ++ P  LVSI+Y+DPG +   ++ GA++ ++L  ++L+ + AA++ Q L+A++GVVT
Sbjct: 38  FFSYLGPGFLVSIAYIDPGNFETDLQSGAQYKYELLWIILVASCAALVIQSLAANLGVVT 97

Query: 119 GRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGLNLLLGWDLFTCVLLTGV 178
           G+ LA+ C  EY K     L + AE++V+  D+  ++G +  LN+L    ++  VLLTG 
Sbjct: 98  GKHLAEHCRAEYSKVPNFMLWVVAEIAVVACDIPEVIGTAFALNMLFNIPVWIGVLLTG- 157

Query: 179 VAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPE-IPLSMNLMLTRLNGESA 238
           ++ L  L        + +F+   +   I L   + +  S+P+   +   L + +L G  A
Sbjct: 158 LSTLILLALQQYGIRKLEFLIAFLVFTIALCFFVELHYSKPDPKEVLYGLFVPQLKGNGA 217

Query: 239 FTL-MSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLFAIVCIFSGIYVVNNV 298
             L +SLLGA VMPHN ++HS++V  ++        +  C  +L          +++N  
Sbjct: 218 TGLAISLLGAMVMPHNLFLHSALVLSRKIPRSVTGIKEACRYYLIESGLALMVAFLINVS 277

Query: 299 LMNSAANVFYSSGLALHTFPDA--LSLMEQVFRSPMVY-----VLFLLVLFLSNQITALT 358
           +++ +  V  +S L+         L L +  F    V       LF + L  S Q + +T
Sbjct: 278 VISVSGAVCNASDLSPEDRASCQDLDLNKASFLLRNVVGKWSSKLFAIALLASGQSSTIT 337

Query: 359 WSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIFSQVMVALL 418
            +  GQ V+  FL L +  WL     R +AIIP+L      G+ G  +L+I + ++++  
Sbjct: 338 GTYAGQYVMQGFLDLRLEPWLRNFLTRCLAIIPSLIVALIGGSAGAGKLIIIASMILSFE 397

Query: 419 LPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMF 460
           LP +++PL +  SS+  MG+   S ++  +  +I   I+G+ I ++V  F
Sbjct: 398 LPFALVPLLKFTSSKTKMGSHANSLVISSVTWIIGGLIMGINIYYLVSSF 446

BLAST of Carg25786 vs. TAIR 10
Match: AT1G80830.1 (natural resistance-associated macrophage protein 1 )

HSP 1 Score: 143.3 bits (360), Expect = 1.4e-33
Identity = 115/410 (28.05%), Postives = 209/410 (50.98%), Query Frame = 0

Query: 59  FMPFIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFNLAAILCQYLSASIGVVT 118
           F  ++ P  LVSI+Y+DPG +   ++ GA + ++L  ++L+ + AA++ Q L+A++GVVT
Sbjct: 46  FFAYLGPGFLVSIAYIDPGNFETDLQAGAHYKYELLWIILVASCAALVIQSLAANLGVVT 105

Query: 119 GRGLAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGLNLLLGWDLFTCVLLTGV 178
           G+ LA+ C  EY K     L + AE++V+  D+  ++G +  LN+L    ++  VLLTG 
Sbjct: 106 GKHLAEQCRAEYSKVPNFMLWVVAEIAVVACDIPEVIGTAFALNMLFSIPVWIGVLLTG- 165

Query: 179 VAALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPEI-PLSMNLMLTRLNGESA 238
           ++ L  L        + +F+   +   I +   + +  S+P+   +   L + +L G  A
Sbjct: 166 LSTLILLALQKYGVRKLEFLIAFLVFTIAICFFVELHYSKPDPGEVLHGLFVPQLKGNGA 225

Query: 239 FTL-MSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLFAIVCIFSGIYVVNNV 298
             L +SLLGA VMPHN ++HS++V  ++        +  C  +L          +++N  
Sbjct: 226 TGLAISLLGAMVMPHNLFLHSALVLSRKIPRSASGIKEACRFYLIESGLALMVAFLINVS 285

Query: 299 LMNSAANVFYSSGLALHTFPDA--LSLMEQVFRSPMVY-----VLFLLVLFLSNQITALT 358
           +++ +  V  +  L+     +   L L +  F    V       LF + L  S Q + +T
Sbjct: 286 VISVSGAVCNAPNLSPEDRANCEDLDLNKASFLLRNVVGKWSSKLFAIALLASGQSSTIT 345

Query: 359 WSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALYCVWSSGAEGMYQLLIFSQVMVALL 418
            +  GQ V+  FL L +  WL     R +AIIP+L      G+ G  +L+I + ++++  
Sbjct: 346 GTYAGQYVMQGFLDLRLEPWLRNLLTRCLAIIPSLIVALIGGSAGAGKLIIIASMILSFE 405

Query: 419 LPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMF 460
           LP +++PL +  S +  MG+      +  +  VI   I+G+ I ++V  F
Sbjct: 406 LPFALVPLLKFTSCKTKMGSHVNPMAITALTWVIGGLIMGINIYYLVSSF 454

BLAST of Carg25786 vs. TAIR 10
Match: AT4G18790.1 (NRAMP metal ion transporter family protein )

HSP 1 Score: 119.4 bits (298), Expect = 2.2e-26
Identity = 106/413 (25.67%), Postives = 203/413 (49.15%), Query Frame = 0

Query: 62  FIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFNLAAILCQYLSASIGVVTGRG 121
           F  P  L+SI+++DPG     ++ GA  G+ L  L+L   L  +L Q LSA IGV TGR 
Sbjct: 73  FTGPGFLMSIAFLDPGNIEGDLQAGAVAGYSLLWLLLWATLMGLLMQLLSARIGVATGRH 132

Query: 122 LAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGLNLLLGWDLFTCVLLTGVVAA 181
           LA+IC  EY     I L   AEV++I  D+  ++G +  L +L    L    +  GV+  
Sbjct: 133 LAEICRSEYPSWARILLWFMAEVALIGADIQEVIGSAIALQILTRGFL---PIWVGVIIT 192

Query: 182 LFPLF--ANLLEDGRAKF--MYICMAGFILLSLVLGVLISQPEI-PLSMNLMLTRLNGES 241
            F  F  + L + G  K   ++  +   + LS       ++P +  L + +++ +L  ++
Sbjct: 193 SFDCFLISYLEKCGMRKLEGLFAVLIATMALSFAWMFNETKPSVEELFIGIIIPKLGSKT 252

Query: 242 AFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPNISKEVLCNNHLFAIVCIFSGIYVVNNV 301
               + ++G  + PHN ++HS++VQ ++  P  I++     N+      I S   +  + 
Sbjct: 253 IREAVGVVGCVITPHNVFLHSALVQSRKTDPKEINRVQEALNY----YTIESSAALFVSF 312

Query: 302 LMNSAANVFYSSGLALHTFPDALSL------MEQVFRSPMVYVLFL--LVLFLSNQITAL 361
           ++N      ++ G       D++ L      +++ +   +  +L++  + L  + Q + +
Sbjct: 313 MINLFVTAVFAKGFYGTKQADSIGLVNAGYYLQEKYGGGVFPILYIWGIGLLAAGQSSTI 372

Query: 362 TWSLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALY-CVWSSGAEGMYQLL-IFSQVMV 421
           T +  GQ ++  FL L +  WL     R  AI+P ++  +  + +EG   +L  +  ++ 
Sbjct: 373 TGTYAGQFIMEGFLDLQMEQWLSAFITRSFAIVPTMFVAIMFNTSEGSLDVLNEWLNILQ 432

Query: 422 ALLLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMF 460
           ++ +P +VIPL  + S+  IMG FKI   +E +A  + V ++ +    +++ F
Sbjct: 433 SMQIPFAVIPLLTMVSNEHIMGVFKIGPSLEKLAWTVAVFVMMINGYLLLDFF 478

BLAST of Carg25786 vs. TAIR 10
Match: AT5G67330.1 (natural resistance associated macrophage protein 4 )

HSP 1 Score: 117.1 bits (292), Expect = 1.1e-25
Identity = 110/438 (25.11%), Postives = 211/438 (48.17%), Query Frame = 0

Query: 62  FIAPALLVSISYVDPGKWAATVEGGARFGFDLFVLVLLFNLAAILCQYLSASIGVVTGRG 121
           F  P  L+SI+++DPG   + ++ GA  G+ L  L++      +L Q LSA +GV TGR 
Sbjct: 55  FTGPGFLMSIAFLDPGNLESDLQAGAIAGYSLIWLLMWATAIGLLIQLLSARLGVATGRH 114

Query: 122 LAQICSEEYDKCTCIFLGIQAEVSVILLDLTMILGISHGLNLLLGW--DLFTCVLLTGVV 181
           LA++C EEY     + L I AE+++I  D+  ++G +  + +L      L+  V++T + 
Sbjct: 115 LAELCREEYPTWARMVLWIMAEIALIGADIQEVIGSAIAIKILSNGLVPLWAGVVITALD 174

Query: 182 AALFPLFANLLEDGRAKFMYICMAGFILLSLVLGVLISQPE---IPLSMNLMLTRLNGES 241
             +F    N    G  K   +       ++L    +  Q +     L +  ++ +L+  +
Sbjct: 175 CFIFLFLENY---GIRKLEAVFAILIATMALAFAWMFGQTKPSGTELLVGALVPKLSSRT 234

Query: 242 AFTLMSLLGASVMPHNFYVHSSIVQQQQQSPPN--ISKEVLCNNHLFAIVCIFSGIYVVN 301
               + ++G  +MPHN ++HS++VQ ++  P      KE L   +        +  +++N
Sbjct: 235 IKQAVGIVGCIIMPHNVFLHSALVQSREVDPKKRFRVKEAL-KYYSIESTGALAVSFIIN 294

Query: 302 NVLMNSAANVFYSSGLA-LHTFPDALSLMEQVFRS---PMVYVLFLLVLFLSNQITALTW 361
             +    A  FY + +A      +A   ++  +     P++Y+  + VL  + Q + +T 
Sbjct: 295 VFVTTVFAKSFYGTEIADTIGLANAGQYLQDKYGGGFFPILYIWAIGVL-AAGQSSTITG 354

Query: 362 SLGGQLVLTNFLKLDIPGWLHCATIRIIAIIPALY--CVWSSGAEGMYQLLIFSQVMVAL 421
           +  GQ ++  FL L +  W+     R  AIIP +    V+ S    + +L  +  V+ ++
Sbjct: 355 TYAGQFIMGGFLNLKMKKWVRALITRSCAIIPTMIVALVFDSSDSMLDELNEWLNVLQSV 414

Query: 422 LLPSSVIPLFRVASSRPIMGAFKISQLVEFIAIVIFVGILGLKIVFVVEMFFGNSDWVVN 481
            +P +VIPL  + S+  IMG+FKI  LV+ I+ ++   ++ +    +V+ F G +  ++ 
Sbjct: 415 QIPFAVIPLLCLVSNEQIMGSFKIQPLVQTISWIVAALVIAINGYLMVDFFSGAATNLI- 474

Query: 482 LRWNMGSGLSIPYVFFLI 487
                   L +P + F I
Sbjct: 475 --------LLVPVIIFAI 478