Carg25483 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg25483 vs. NCBI nr
Match: KAG7011019.1 (BUD13-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 971.1 bits (2509), Expect = 3.7e-279
Identity = 505/505 (100.00%), Postives = 505/505 (100.00%), Query Frame = 0

Query: 1   MSAKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNALDEEPQVDEDIEVTRMRRLE 60
           MSAKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNALDEEPQVDEDIEVTRMRRLE
Sbjct: 1   MSAKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNALDEEPQVDEDIEVTRMRRLE 60

Query: 61  ELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGDLSPPRRTRARNDTPSPSNELKPSVSG 120
           ELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGDLSPPRRTRARNDTPSPSNELKPSVSG
Sbjct: 61  ELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGDLSPPRRTRARNDTPSPSNELKPSVSG 120

Query: 121 EEDIDLSPPRQRRKRYHTPSPESDVKPARSVSPQSDLSPPRRSDRQASKANLGGNHKAAG 180
           EEDIDLSPPRQRRKRYHTPSPESDVKPARSVSPQSDLSPPRRSDRQASKANLGGNHKAAG
Sbjct: 121 EEDIDLSPPRQRRKRYHTPSPESDVKPARSVSPQSDLSPPRRSDRQASKANLGGNHKAAG 180

Query: 181 LSDLSPPRRRTSGYADGAHISHESDLSPPRKQSKDVRRDRSLSDKHLQSHIVTDDSQESP 240
           LSDLSPPRRRTSGYADGAHISHESDLSPPRKQSKDVRRDRSLSDKHLQSHIVTDDSQESP
Sbjct: 181 LSDLSPPRRRTSGYADGAHISHESDLSPPRKQSKDVRRDRSLSDKHLQSHIVTDDSQESP 240

Query: 241 ADLSPHRKMQKALPVSVSFKQARKTGLLTQKEFGEEMSKTNKEDWTRFKEMNPSASSNAE 300
           ADLSPHRKMQKALPVSVSFKQARKTGLLTQKEFGEEMSKTNKEDWTRFKEMNPSASSNAE
Sbjct: 241 ADLSPHRKMQKALPVSVSFKQARKTGLLTQKEFGEEMSKTNKEDWTRFKEMNPSASSNAE 300

Query: 301 PVYRDKIKERYRRSSFKRGILKSTRKNRRKAQGDKVLTITYMLLSNIIVAACDDLPSQEI 360
           PVYRDKIKERYRRSSFKRGILKSTRKNRRKAQGDKVLTITYMLLSNIIVAACDDLPSQEI
Sbjct: 301 PVYRDKIKERYRRSSFKRGILKSTRKNRRKAQGDKVLTITYMLLSNIIVAACDDLPSQEI 360

Query: 361 KLEWGKGLAQKREAEAERMELELEKDRPFARSSNDAELDSMLRERLRWGDPMAHLVKKKQ 420
           KLEWGKGLAQKREAEAERMELELEKDRPFARSSNDAELDSMLRERLRWGDPMAHLVKKKQ
Sbjct: 361 KLEWGKGLAQKREAEAERMELELEKDRPFARSSNDAELDSMLRERLRWGDPMAHLVKKKQ 420

Query: 421 SEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRGLDAAPNRYGIRPGRHWDGVDRSNGFE 480
           SEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRGLDAAPNRYGIRPGRHWDGVDRSNGFE
Sbjct: 421 SEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRGLDAAPNRYGIRPGRHWDGVDRSNGFE 480

Query: 481 KQMLKRMNEKRATEREAYLWSVSDM 506
           KQMLKRMNEKRATEREAYLWSVSDM
Sbjct: 481 KQMLKRMNEKRATEREAYLWSVSDM 505

BLAST of Carg25483 vs. NCBI nr
Match: XP_022944665.1 (LOW QUALITY PROTEIN: BUD13 homolog [Cucurbita moschata])

HSP 1 Score: 814.7 bits (2103), Expect = 4.5e-232
Identity = 457/580 (78.79%), Postives = 460/580 (79.31%), Query Frame = 0

Query: 1   MSAKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNAL------------------- 60
           MSAKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNAL                   
Sbjct: 1   MSAKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNALGXLVVDEDPVWQKPIIIEE 60

Query: 61  -------DEEPQVDEDIEVTRMRRLEELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGD 120
                  DEEPQVDEDIEVTRMRRLEELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGD
Sbjct: 61  DNVDNSTDEEPQVDEDIEVTRMRRLEELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGD 120

Query: 121 LSPPRRTRARNDTPSPSNELKPSVSGEE-------------------------------- 180
           LSP RRTRARNDTPSPSNELKPSVSGEE                                
Sbjct: 121 LSPSRRTRARNDTPSPSNELKPSVSGEEGEDFSPPRRRQRQNPGSLEHEEMSTNSKSPRS 180

Query: 181 -----------------DIDLSPPRQRRKRYHTPSPESDVKPARSVSPQSDLSPPRRSDR 240
                            DIDLSPPRQRRKRYHTPSPESDVKPARSVSPQSDLSPPRRSDR
Sbjct: 181 DLSQPQKQNVYRDCAQKDIDLSPPRQRRKRYHTPSPESDVKPARSVSPQSDLSPPRRSDR 240

Query: 241 QASKANLGGNHKAAGLSDLSPPRRRTSGYADGAHISHESDLSPPRKQSKDVRRDRSLSDK 300
           QASKANLGGNHKAAGLSDLSPPRRRTSGYADGAHISH SDLSPPRKQSKDVRRDRSLSDK
Sbjct: 241 QASKANLGGNHKAAGLSDLSPPRRRTSGYADGAHISHGSDLSPPRKQSKDVRRDRSLSDK 300

Query: 301 HLQSHIVTDDSQESPADLSPHRKMQKALPVSVSFKQARKTGLLTQKEFGEEMSKTNKEDW 360
           HLQSHIVTDDSQESPADLSPHRKMQKALPVSVSFKQARKTGLLTQKEFGEEMSKTNKEDW
Sbjct: 301 HLQSHIVTDDSQESPADLSPHRKMQKALPVSVSFKQARKTGLLTQKEFGEEMSKTNKEDW 360

Query: 361 TRFKEMNPSASSNAEPVYRDKIKERYRRSSFKRGILKSTRKNRRKAQGDKVLTITYMLLS 420
           TRFKEMNPSASSNAEPVYRDKIK     +  K   LK+  K   K               
Sbjct: 361 TRFKEMNPSASSNAEPVYRDKIK---GXAIDKEEFLKARGKIEEK--------------- 420

Query: 421 NIIVAACDDLPSQEIKLEWGKGLAQKREAEAERMELELEKDRPFARSSNDAELDSMLRER 480
                       +EIKLEWGKGLAQKREAEAERMELELEKDRPFARSSNDAELDSMLRER
Sbjct: 421 -----------PKEIKLEWGKGLAQKREAEAERMELELEKDRPFARSSNDAELDSMLRER 480

Query: 481 LRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRGLDAAPNRYGIR 506
           LRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRGLDAAPNRYGIR
Sbjct: 481 LRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRGLDAAPNRYGIR 540

BLAST of Carg25483 vs. NCBI nr
Match: KAG6571223.1 (BUD13-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 745.7 bits (1924), Expect = 2.6e-211
Identity = 420/533 (78.80%), Postives = 429/533 (80.49%), Query Frame = 0

Query: 1   MSAKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNALDEEPQVDEDIEVTRMRRLE 60
           MSAKSKSLKEYLKRYESNTE       K          N+ DEEPQVDEDIEVTRMRRLE
Sbjct: 1   MSAKSKSLKEYLKRYESNTE-------KPIIIEEDNVDNSTDEEPQVDEDIEVTRMRRLE 60

Query: 61  ELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGDLSPPRRTRARNDTPSPSNELKPSVSG 120
           ELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGDLSPPRRTRARNDTPSPSNELKPSVSG
Sbjct: 61  ELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGDLSPPRRTRARNDTPSPSNELKPSVSG 120

Query: 121 EEDIDLSPPRQRRKRYHTPSPESDVKPARSVSPQ-------------------------- 180
           EE  D SPPR RR+R +  S E +  P  S+S Q                          
Sbjct: 121 EEGEDFSPPR-RRQRQNPGSLEHEEMPTNSISLQDLTYLNHKSRMFIEIVHRKILTYHLH 180

Query: 181 -----SDLSPPRRSDRQASKANLGGNHKAAGLSDLSPPRRRTSGYADGAHISHESDLSPP 240
                SDLSPPRRSDRQASKANLGGNHKAAGLSDLSPPRRRTSGYADGAHISHESDLSPP
Sbjct: 181 DSEGNSDLSPPRRSDRQASKANLGGNHKAAGLSDLSPPRRRTSGYADGAHISHESDLSPP 240

Query: 241 RKQSKDVRRDRSLSDKHLQSHIVTDDSQESPADLSPHRKMQKALPVSVSFKQARKTGLLT 300
           RKQSKDVRRDRSLSDKHLQSHIVTDDSQESPADLSPHRKMQKALPVSVSFKQARKTGLLT
Sbjct: 241 RKQSKDVRRDRSLSDKHLQSHIVTDDSQESPADLSPHRKMQKALPVSVSFKQARKTGLLT 300

Query: 301 QKEFGEEMSKTNKEDWTRFKEMNPSASSNAEPVYRDKIK-ERYRRSSFKRGILKSTRKNR 360
           QKEFGEEMSKTNKEDWTRFKEMNPSASSNAEPVYRDKIK +R  +  F    LK+  K  
Sbjct: 301 QKEFGEEMSKTNKEDWTRFKEMNPSASSNAEPVYRDKIKGDRVSKEEF----LKARGKIE 360

Query: 361 RKAQGDKVLTITYMLLSNIIVAACDDLPSQEIKLEWGKGLAQKREAEAERMELELEKDRP 420
            K                           +EIKLEWGKGLAQKREAEAERMELELEKDRP
Sbjct: 361 EK--------------------------PKEIKLEWGKGLAQKREAEAERMELELEKDRP 420

Query: 421 FARSSNDAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPH 480
           FARSSNDAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPH
Sbjct: 421 FARSSNDAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPH 480

Query: 481 SWLKRGLDAAPNRYGIRPGRHWDGVDRSNGFEKQMLKRMNEKRATEREAYLWS 502
           SWLKRGLDAAPNRYGIRPGRHWDGVDRSNGFEKQMLKRMNEKRATEREAYLWS
Sbjct: 481 SWLKRGLDAAPNRYGIRPGRHWDGVDRSNGFEKQMLKRMNEKRATEREAYLWS 495

BLAST of Carg25483 vs. NCBI nr
Match: XP_038901025.1 (BUD13 homolog [Benincasa hispida] >XP_038901026.1 BUD13 homolog [Benincasa hispida] >XP_038901027.1 BUD13 homolog [Benincasa hispida] >XP_038901028.1 BUD13 homolog [Benincasa hispida])

HSP 1 Score: 723.4 bits (1866), Expect = 1.4e-204
Identity = 418/592 (70.61%), Postives = 432/592 (72.97%), Query Frame = 0

Query: 1   MSAKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNAL------------------- 60
           MSAKSKSLKEYLKRYESNTE DKKKKKKKKRT  ATKPNAL                   
Sbjct: 2   MSAKSKSLKEYLKRYESNTEEDKKKKKKKKRTTAATKPNALGVLVVDEDPIWQKPIIIEE 61

Query: 61  -------DEEPQVDEDIEVTRMRRLEELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGD 120
                  DEEPQVDEDIEV RMRRLEELKAKRPYNSISEDGSGWVSLSPNHANSSMVN D
Sbjct: 62  DNADNSTDEEPQVDEDIEVKRMRRLEELKAKRPYNSISEDGSGWVSLSPNHANSSMVNSD 121

Query: 121 LSPPRRTRARNDTPSPSNELKPSVSGE--------------------------------- 180
           +SPPRRTR RNDTPSPSNELKP VSGE                                 
Sbjct: 122 MSPPRRTRVRNDTPSPSNELKPPVSGEEGEDFSSPRRRQRQHPSSLEHDENPTNSIYPPS 181

Query: 181 ---------------------------EDIDLSPPRQRRKRYHTPSPESDVKPARSVSPQ 240
                                      EDIDLSPPRQRRKRYHTPSPE DV    SVSPQ
Sbjct: 182 SSPPQKQNVYRDTHLQGAIPEHMDCAQEDIDLSPPRQRRKRYHTPSPEPDVTHTHSVSPQ 241

Query: 241 SDLSPPRRSDRQASKANLGGNHKAAGLSDLSPPRRRTSGYADGAHISHESDLSPPRKQSK 300
           SD+SPPRRSDRQASKA+LGGNHKAAGLSDLS PRRRTS YAD A+IS   DLSPPRKQ K
Sbjct: 242 SDMSPPRRSDRQASKASLGGNHKAAGLSDLSLPRRRTSDYADDAYISRGPDLSPPRKQRK 301

Query: 301 DVRRDRSLSDKHLQSHIVTDDSQESPADLSPHRKMQKALPVSVSFKQARKTGLLTQKEFG 360
           DVR DRSLSDK  ++H VTD S E  ADLSP RK QKALPVSVSFKQARKTGLLTQ+EFG
Sbjct: 302 DVRGDRSLSDKPSRNH-VTDASPELLADLSPRRKQQKALPVSVSFKQARKTGLLTQQEFG 361

Query: 361 EEMSKTNKEDWTRFKEMNPSASSNAEPVYRDKIK-ERYRRSSFKRGILKSTRKNRRKAQG 420
           EEMSKTNKEDWTRFKEMNPSAS NAEPVYRDKIK +R  +  F    LK+  K   K   
Sbjct: 362 EEMSKTNKEDWTRFKEMNPSASGNAEPVYRDKIKGDRISKEEF----LKARGKKEEK--- 421

Query: 421 DKVLTITYMLLSNIIVAACDDLPSQEIKLEWGKGLAQKREAEAERMELELEKDRPFARSS 480
                                   +EIKLEWGKGLAQKREAEAE MELELEKDRPFARS 
Sbjct: 422 -----------------------PKEIKLEWGKGLAQKREAEAELMELELEKDRPFARSR 481

Query: 481 NDAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKR 506
           NDAELDSMLR+RLRWGDPMAHLVKK+QSEMALPDLGDSEKMKESGFIIPQ+IPPHSWLKR
Sbjct: 482 NDAELDSMLRDRLRWGDPMAHLVKKRQSEMALPDLGDSEKMKESGFIIPQEIPPHSWLKR 541

BLAST of Carg25483 vs. NCBI nr
Match: KAG6596913.1 (BUD13-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030189.1 BUD13-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 701.8 bits (1810), Expect = 4.2e-198
Identity = 402/591 (68.02%), Postives = 431/591 (72.93%), Query Frame = 0

Query: 1   MSAKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNAL------------------- 60
           MS K KSLKEYLKRYE+NT+ DKKKKKKKKRTATATKPNAL                   
Sbjct: 1   MSEKLKSLKEYLKRYETNTDEDKKKKKKKKRTATATKPNALGVLVVDEDPVWQKQIVIEE 60

Query: 61  -------DEEPQVDEDIEVTRMRRLEELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGD 120
                  DEEPQVDEDIEV RMRRLEE+KAKRPYNSISEDGSGWVSL PN ANS++V+ D
Sbjct: 61  DSAHNSTDEEPQVDEDIEVKRMRRLEEVKAKRPYNSISEDGSGWVSLPPNPANSNVVSSD 120

Query: 121 LSPPRRTRARNDTPSPSNELKP-------------------------------------- 180
           +SPPRRTRARNDTPSPS+ELKP                                      
Sbjct: 121 ISPPRRTRARNDTPSPSHELKPPGEEAEDISPPRRRQRKHPSSLEHDEKATSSTYPSSDS 180

Query: 181 ----------------SVSG-----EEDIDLSPPRQRRKRYHTPSPESDVKPARSVSPQS 240
                           + SG     +EDIDLSPPRQRRKRYHTPSPE D KP +  SPQS
Sbjct: 181 SPPRKQNVYRGTHLQGADSGHLDRAQEDIDLSPPRQRRKRYHTPSPEPDGKPTQPFSPQS 240

Query: 241 DLSPPRRSDRQASKANLGGNHKAAGLSDLSPPRRRTSGYADGAHISHESDLSPPRKQSKD 300
           D+SPPRR DRQASK++LG NHKAAGLSDLSPPRRRTS YA+ A++S  SDLSPPRKQ  D
Sbjct: 241 DMSPPRRVDRQASKSSLGVNHKAAGLSDLSPPRRRTSNYAEDAYVSRGSDLSPPRKQRID 300

Query: 301 VRRDRSLSDKHLQSHIVTDDSQESPADLSPHRKMQKALPVSVSFKQARKTGLLTQKEFGE 360
           VR DR L++ + Q+H+VTD SQESP DLSP RK QK  PVSVSFKQ RKTGLLTQ+EFGE
Sbjct: 301 VRGDRLLTENNSQNHVVTDASQESPPDLSPQRKKQKVPPVSVSFKQPRKTGLLTQQEFGE 360

Query: 361 EMSKTNKEDWTRFKEMNPSASSNAEPVYRDKIK-ERYRRSSFKRGILKSTRKNRRKAQGD 420
           EMSKTNKEDWTRFKEMNPSASSNAEP+YRDKIK +R  +  F    LKS  K   K    
Sbjct: 361 EMSKTNKEDWTRFKEMNPSASSNAEPIYRDKIKGDRISKEEF----LKSRGKIEEK---- 420

Query: 421 KVLTITYMLLSNIIVAACDDLPSQEIKLEWGKGLAQKREAEAERMELELEKDRPFARSSN 480
                                  +EIKLEWGKGLAQKREAEAE+MELELEKDRPFARS N
Sbjct: 421 ----------------------PKEIKLEWGKGLAQKREAEAEQMELELEKDRPFARSRN 480

Query: 481 DAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRG 506
           DAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRG
Sbjct: 481 DAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRG 540

BLAST of Carg25483 vs. ExPASy Swiss-Prot
Match: Q8R149 (BUD13 homolog OS=Mus musculus OX=10090 GN=Bud13 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 4.2e-31
Identity = 144/492 (29.27%), Postives = 200/492 (40.65%), Query Frame = 0

Query: 94  DLSPPRRTRARNDTPSPSNELKPSVSGEEDIDLSPPR----------------------- 153
           DLSPPR  R R+DTP PS    P      D+D SPPR                       
Sbjct: 211 DLSPPR--RVRHDTPDPS----PPRRVRHDLDASPPRKSHRNSSAVSPRRGHHGSLGTSS 270

Query: 154 -----------------------QRRKRYHTPSPESDVKPARS----------------- 213
                                  Q  +R H  SP+ ++  A+S                 
Sbjct: 271 PRQTHNHSPTAAQHRRTLDSSGTQHLRRAHHESPDLELHKAKSSKAAERAPSKAASQSGL 330

Query: 214 ------------VSPQSDLSPPRRSDRQA-----SKANLGGNHKAAGLSDLSPPRRRTSG 273
                           SDLSPPR+   +A      + +  G ++ A  SDLSPPR++   
Sbjct: 331 GPSHPSLSTNSKYEHDSDLSPPRKRQAKAHFEAKKQLDSKGVYQKASDSDLSPPRKK--- 390

Query: 274 YADGAHISHESDLSPPRKQSKDVRRDRSLSDKHLQSHIVTDDSQESPADLSPHRKMQKAL 333
             +  H   +SDLSPPR + +    D  LS    +    + DS     DLSP R+  +  
Sbjct: 391 -KNSGHQDSDSDLSPPRNRPRRQSSDSDLSPPRRRQRTKSSDS-----DLSPPRRSPRPG 450

Query: 334 PVSVSFKQARKTGLLTQKEFGEEMSKTNKEDWTRFKEMNPSASSNAEPVYRDKIKERYRR 393
             +       KTGL+T  +   +  K   +D T       +     E V+RDK       
Sbjct: 451 KKTAHMYSGAKTGLVTDVQREHQELKKQDQDTTDL----GAQFEFTETVFRDK------- 510

Query: 394 SSFKRGILKSTRKNRRKAQGDKVLTITYMLLSNIIVAACDDLPSQEIKLEWGKGLAQKRE 453
           S  KR +     + RRKA+ D                        E+  +WGKGLAQ R+
Sbjct: 511 SGRKRNLKLERLEQRRKAEKDS--------------------ERDELYAQWGKGLAQSRQ 570

Query: 454 AEAERMELELEKDRPFARSSNDAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEK 506
            +    +   E  +P AR  +D +LD MLRE+ R GDPMA+ +KK +++        ++K
Sbjct: 571 QQQNVEDAMKEMQKPLARYIDDEDLDRMLREQEREGDPMANFIKKNKAKE-----NKNKK 630

BLAST of Carg25483 vs. ExPASy Swiss-Prot
Match: Q4QQU1 (BUD13 homolog OS=Rattus norvegicus OX=10116 GN=Bud13 PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 1.2e-30
Identity = 126/410 (30.73%), Postives = 182/410 (44.39%), Query Frame = 0

Query: 97  PPRRTRARNDTPSPSNELKPSVSGEEDIDLSPPRQRRKRYHTPSPES-DVKPARSVSPQS 156
           P  +T +++    P   L  +   E D DLSPPR+R+ + H  + +  D K     +  S
Sbjct: 313 PSSKTVSQSGLGPPHPSLSMNSKYEHDSDLSPPRKRQAKAHFGAKKQLDSKGVCQKASDS 372

Query: 157 DLSPPRRSDRQASKANLGGNHKAAGLSDLSPPRRRTSGYADGAHISHESDLSPPRKQSKD 216
           DLSPPR++ +          H+ +  SDLSPPR R          S +SDLSPPR++ + 
Sbjct: 373 DLSPPRKNSK--------SGHQDSD-SDLSPPRNRP------RRQSSDSDLSPPRRRQR- 432

Query: 217 VRRDRSLSDKHLQSHIVTDDSQESPADLSPHRKMQKALPVSVSFKQARKTGLLTQKEFGE 276
                               ++ S +DLSP R+  ++   +       KTGL+   +   
Sbjct: 433 --------------------TKSSDSDLSPSRRSPRSGKKTPHMYSGAKTGLVADVQREH 492

Query: 277 EMSKTNKEDWTRFKEMNPSASSNAEPVYRDKIKERYRRSSFKRGILKSTRKNRRKAQGDK 336
           +  K   +D T       +     E V+RDK       S  KR +     + RRKA+ D 
Sbjct: 493 QELKKQDQDATDL----GAQFEFTETVFRDK-------SGRKRNLKLERLEQRRKAEKDS 552

Query: 337 VLTITYMLLSNIIVAACDDLPSQEIKLEWGKGLAQKREAEAERMELELEKDRPFARSSND 396
                                  E+  +WGKGLAQ R+ +    +   E  +P AR  +D
Sbjct: 553 --------------------ERDELYAQWGKGLAQSRQQQQNVEDAMKEMQKPLARYIDD 612

Query: 397 AELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRGL 456
            +LD MLRE+ R GDPMA+ +KK +++          K K+         PP        
Sbjct: 613 EDLDRMLREQEREGDPMANFIKKNKAK--------ENKNKKVRPRYNGPAPP-------- 636

Query: 457 DAAPNRYGIRPGRHWDGVDRSNGFEKQMLKRMNEKRATEREAYLWSVSDM 506
              PNR+ I PG  WDGVDRSNGFE++   R+  K+A E  AY WSV DM
Sbjct: 673 ---PNRFNIWPGYRWDGVDRSNGFEQKRFARLASKKAVEELAYKWSVEDM 636

BLAST of Carg25483 vs. ExPASy Swiss-Prot
Match: Q9BRD0 (BUD13 homolog OS=Homo sapiens OX=9606 GN=BUD13 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 3.7e-27
Identity = 137/455 (30.11%), Postives = 192/455 (42.20%), Query Frame = 0

Query: 94  DLSPPRRTR-ARNDTPSP------SNELKPSVSGEEDIDL-------SPPRQRRKRYHTP 153
           D SPPRR R   +D  SP      S +      G  D          SP       Y  P
Sbjct: 224 DPSPPRRARHGSSDISSPRRVHNNSPDTSRRTLGSSDTQQLRRARHDSPDLAPNVTYSLP 283

Query: 154 SPESDVKPARSVSPQS-----------------------DLSPPRRSDRQA-----SKAN 213
             +S   P R+ S  S                       D+SPPR+   ++      + +
Sbjct: 284 RTKSGKAPERASSKTSPHWKESGASHLSFPKNSKYEYDPDISPPRKKQAKSHFGDKKQLD 343

Query: 214 LGGNHKAAGLSDLSPPRRRTSGYADGAHISHESDLSPPRKQSKDVRRDRSLSDKHLQSHI 273
             G+ + A  SDLS PR + S      H   +SDLSPPR + +    D  LS    +   
Sbjct: 344 SKGDCQKATDSDLSSPRHKQS----PGHQDSDSDLSPPRNRPRHRSSDSDLSPPRRRQRT 403

Query: 274 VTDDSQESPADLSPHRKMQKALPVSVSFKQARKTGL-LTQKEFGEEMSKTNKEDWTRFKE 333
            + DS     DLSP R+ Q     +       KTGL LT  +  ++  K   ++   F+ 
Sbjct: 404 KSSDS-----DLSPPRRSQPPGKKAAHMYSGAKTGLVLTDIQREQQELKEQDQETMAFE- 463

Query: 334 MNPSASSNAEPVYRDKIKERYRRSSFKRGILKSTRKNRRKAQGDKVLTITYMLLSNIIVA 393
              +    AE V+RDK       S  KR +     + RRKA+ D                
Sbjct: 464 ---AEFQYAETVFRDK-------SGRKRNLKLERLEQRRKAEKDS--------------- 523

Query: 394 ACDDLPSQEIKLEWGKGLAQKREAEAERMELELEKDRPFARSSNDAELDSMLRERLRWGD 453
                   E+  +WGKGLAQ R+ +    +   E  +P AR  +D +LD MLRE+ R GD
Sbjct: 524 -----ERDELYAQWGKGLAQSRQQQQNVEDAMKEMQKPLARYIDDEDLDRMLREQEREGD 583

Query: 454 PMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRGLDAAPNRYGIRPGRHW 506
           PMA+ +KK +++        ++K++          P +S    G    PNR+ I PG  W
Sbjct: 584 PMANFIKKNKAKE-----NKNKKVR----------PRYS----GPAPPPNRFNIWPGYRW 619

BLAST of Carg25483 vs. ExPASy Swiss-Prot
Match: Q5ZIJ0 (BUD13 homolog OS=Gallus gallus OX=9031 GN=BUD13 PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 2.9e-24
Identity = 137/446 (30.72%), Postives = 198/446 (44.39%), Query Frame = 0

Query: 81  LSPNHANSSMVNGDLSPPRRTRARNDTPSPSNELKPSVSGEEDIDLSPPRQRRKRYHTPS 140
           LSP        + DLSPPR  R R+D+P  S    P     +  DLSPPR++R     PS
Sbjct: 179 LSPPRRKRHDDSPDLSPPR--RKRHDSPDLS---PPRRKRHDSPDLSPPRRQRHDSPDPS 238

Query: 141 PESDVK-PARSVSPQS-----DLSPPRR----SDRQAS-----KANLGGNHKAAGLSDLS 200
           P    +  +  +SPQ      DLS  R+    SD  +S     +A   G+ K +   D S
Sbjct: 239 PPRKKRHDSPDLSPQEQQTTPDLSLQRKKRYDSDPDSSPSLRKRARSPGHKKPSRTKDAS 298

Query: 201 PPR--RRTSGYAD---GAHISHESDLSPPRKQSKDVRRDRSLSDKHLQSHIVTDDSQESP 260
           P +  RR S       G   + E+DLSP   Q K+ R   S   +H           +SP
Sbjct: 299 PVKKLRRDSDSPPPRRGTQNASEADLSP---QGKNHRALPSGKGQH---------GSKSP 358

Query: 261 ADLSPHR-KMQKALPVSVSFKQARKTGLLTQKEFGEEMSKTNKEDWTRFKEMNPSASSNA 320
            DLS H     K  P   +     + GL++      E  +  K++  R  +     S + 
Sbjct: 359 PDLSRHHYPDDKGSPKKGNMMSGVRAGLVSADVLRREQQELRKQE--RSSKHLEEESRHT 418

Query: 321 EPVYRDKIKERYRRSSFKRGILKSTRKNRRKAQGDKVLTITYMLLSNIIVAACDDLPSQE 380
           E ++RDK     R+    +  L+  +K+  K++ D                        E
Sbjct: 419 ETIFRDK---SGRKRDLVQERLEQQQKDEAKSERD------------------------E 478

Query: 381 IKLEWGKGLAQKREAEAERMELELEKDRPFARSSNDAELDSMLRERLRWGDPMAHLVKKK 440
               WG+GLAQ R+ +    +   E  +P AR  +D +LD MLRE+ R GDPMA  ++K+
Sbjct: 479 QYARWGRGLAQGRQQQQNVEDAIKEMQKPLARYIDDEDLDRMLREQEREGDPMAEFIRKR 538

Query: 441 QSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRGLDAAPNRYGIRPGRHWDGVDRSNGF 500
           +++       +S++ KE         P +S    G     NR+ I PG  WDGVDRSNGF
Sbjct: 539 KAK-------ESKEKKEK--------PRYS----GPAPPLNRFNIWPGHRWDGVDRSNGF 559

Query: 501 EKQMLKRMNEKRATEREAYLWSVSDM 506
           E+Q   RM  K+A +  AY WS+ DM
Sbjct: 599 EQQFFARMANKKAVQELAYKWSIEDM 559

BLAST of Carg25483 vs. ExPASy Swiss-Prot
Match: P30640 (BUD13 homolog OS=Caenorhabditis elegans OX=6239 GN=R08D7.1 PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 1.2e-22
Identity = 146/522 (27.97%), Postives = 232/522 (44.44%), Query Frame = 0

Query: 1   MSAKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNALDEEPQVDEDIEVTR----M 60
           M++K+  LK+YL    ++ +++KKKKKK K     +    ++E+  +  D   TR     
Sbjct: 1   MTSKADYLKKYLS--PASGDIEKKKKKKNKDKNKPSGLRLIEEDAFLSVDAAKTRDIGSD 60

Query: 61  RRLEELKAKRPYNSISEDGSGWVSL-----SPNHANSSMVNGDLSPPRRTRARNDTPSPS 120
              EE++  +     ++   G+        +P       ++ D SPPR  R R+D  S +
Sbjct: 61  EEREEIEVLKQSVKKAKVVHGFKQTFAEVDAPKVIKPEPLSPDNSPPRGKRQRHD--SDN 120

Query: 121 NELKPSVSGEEDIDLSPPRQRRKRYHTPSPESDVKPARSVSPQSDLSPPRRSDRQASKAN 180
           +  +PS     D D SPPR  R R+ +   + D  P R     SD SPPR S +   ++ 
Sbjct: 121 SPPRPS-RKRNDSDNSPPRPSRNRHDS---DKDNSPPRRRRHDSDNSPPRPSRKIREESP 180

Query: 181 LGGNHKAAGLSDLSPPRRRTSGYADGAHISHESDLSPPRKQSKDVRRDRSLSDKHLQSHI 240
              N +       SPPR R           H+SD SPPR +S   RRD   S    +   
Sbjct: 181 SARNRR-------SPPRTRRD--------RHDSDNSPPRNRS---RRDSDNSPPRRRPS- 240

Query: 241 VTDDSQESPADLSPHRKMQKA--------------LPVSVSFKQARKTGLLTQKEFGEEM 300
            +   +    DLSP RK +K                  S    + +++GL + ++  EE 
Sbjct: 241 -SPARRRKDDDLSPPRKSRKIEEPKKIKKEEPDSDTETSGRTLEGKRSGLQSARDLKEES 300

Query: 301 SKTNKEDWTRFKEMNPSASSN-AEPVYRDKIKERYRRSSFKRGILKSTRKNRRKAQGDKV 360
            K   ++   F+EM+ S S   A+ VYR K        + K+G  K + +++ K + +  
Sbjct: 301 DKLRAKNSKMFEEMDTSVSGRFADTVYRQK-------QTKKKG--KDSEEDQAKKERETK 360

Query: 361 LTITYMLLSNIIVAACDDLPSQEIKLEWGKGLAQKREAEAERMELELEKDRPFARSSNDA 420
            T              ++L  +E    W KG+AQ  +  A+  E+      P AR+ +D 
Sbjct: 361 KT--------------EEL--KEKYKSWNKGVAQIEDRRAQLEEMARVAAEPMARARDDD 420

Query: 421 ELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRGLD 480
            +++ L+E L   DPMA++++KK+ + A+ D G+       G  +               
Sbjct: 421 AMNAHLKEVLHAADPMANMIQKKRRDTAI-DRGELVYPSYHGHFV--------------- 451

Query: 481 AAPNRYGIRPGRHWDGVDRSNGFEKQMLKRMNEKRATEREAY 499
             PNR+GI PG  WDGVDRSNGFE ++ K  N K A + E Y
Sbjct: 481 --PNRFGIAPGYRWDGVDRSNGFEGKLAKTENTKTANQSEYY 451

BLAST of Carg25483 vs. ExPASy TrEMBL
Match: A0A6J1FYT1 (LOW QUALITY PROTEIN: BUD13 homolog OS=Cucurbita moschata OX=3662 GN=LOC111449055 PE=3 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 2.2e-232
Identity = 457/580 (78.79%), Postives = 460/580 (79.31%), Query Frame = 0

Query: 1   MSAKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNAL------------------- 60
           MSAKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNAL                   
Sbjct: 1   MSAKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNALGXLVVDEDPVWQKPIIIEE 60

Query: 61  -------DEEPQVDEDIEVTRMRRLEELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGD 120
                  DEEPQVDEDIEVTRMRRLEELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGD
Sbjct: 61  DNVDNSTDEEPQVDEDIEVTRMRRLEELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGD 120

Query: 121 LSPPRRTRARNDTPSPSNELKPSVSGEE-------------------------------- 180
           LSP RRTRARNDTPSPSNELKPSVSGEE                                
Sbjct: 121 LSPSRRTRARNDTPSPSNELKPSVSGEEGEDFSPPRRRQRQNPGSLEHEEMSTNSKSPRS 180

Query: 181 -----------------DIDLSPPRQRRKRYHTPSPESDVKPARSVSPQSDLSPPRRSDR 240
                            DIDLSPPRQRRKRYHTPSPESDVKPARSVSPQSDLSPPRRSDR
Sbjct: 181 DLSQPQKQNVYRDCAQKDIDLSPPRQRRKRYHTPSPESDVKPARSVSPQSDLSPPRRSDR 240

Query: 241 QASKANLGGNHKAAGLSDLSPPRRRTSGYADGAHISHESDLSPPRKQSKDVRRDRSLSDK 300
           QASKANLGGNHKAAGLSDLSPPRRRTSGYADGAHISH SDLSPPRKQSKDVRRDRSLSDK
Sbjct: 241 QASKANLGGNHKAAGLSDLSPPRRRTSGYADGAHISHGSDLSPPRKQSKDVRRDRSLSDK 300

Query: 301 HLQSHIVTDDSQESPADLSPHRKMQKALPVSVSFKQARKTGLLTQKEFGEEMSKTNKEDW 360
           HLQSHIVTDDSQESPADLSPHRKMQKALPVSVSFKQARKTGLLTQKEFGEEMSKTNKEDW
Sbjct: 301 HLQSHIVTDDSQESPADLSPHRKMQKALPVSVSFKQARKTGLLTQKEFGEEMSKTNKEDW 360

Query: 361 TRFKEMNPSASSNAEPVYRDKIKERYRRSSFKRGILKSTRKNRRKAQGDKVLTITYMLLS 420
           TRFKEMNPSASSNAEPVYRDKIK     +  K   LK+  K   K               
Sbjct: 361 TRFKEMNPSASSNAEPVYRDKIK---GXAIDKEEFLKARGKIEEK--------------- 420

Query: 421 NIIVAACDDLPSQEIKLEWGKGLAQKREAEAERMELELEKDRPFARSSNDAELDSMLRER 480
                       +EIKLEWGKGLAQKREAEAERMELELEKDRPFARSSNDAELDSMLRER
Sbjct: 421 -----------PKEIKLEWGKGLAQKREAEAERMELELEKDRPFARSSNDAELDSMLRER 480

Query: 481 LRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRGLDAAPNRYGIR 506
           LRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRGLDAAPNRYGIR
Sbjct: 481 LRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRGLDAAPNRYGIR 540

BLAST of Carg25483 vs. ExPASy TrEMBL
Match: A0A6J1F993 (BUD13 homolog OS=Cucurbita moschata OX=3662 GN=LOC111443156 PE=3 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 2.7e-198
Identity = 402/591 (68.02%), Postives = 431/591 (72.93%), Query Frame = 0

Query: 1   MSAKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNAL------------------- 60
           MS K KSLKEYLKRYE+NT+ DKKKKKKKKRTATATKPNAL                   
Sbjct: 1   MSEKLKSLKEYLKRYETNTDEDKKKKKKKKRTATATKPNALGVLVVDEDPVWQKQIVIEE 60

Query: 61  -------DEEPQVDEDIEVTRMRRLEELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGD 120
                  DEEPQVDEDIEV RMRRLEE+KAKRPYNSISEDGSGWVSL PN ANS++V+ D
Sbjct: 61  DSAHNSTDEEPQVDEDIEVKRMRRLEEVKAKRPYNSISEDGSGWVSLPPNPANSNVVSSD 120

Query: 121 LSPPRRTRARNDTPSPSNELKP-------------------------------------- 180
           +SPPRRTRARNDTPSPS+ELKP                                      
Sbjct: 121 ISPPRRTRARNDTPSPSHELKPPGEEAEDISPPRRRQRKHPSSLEHDEKATSSTYPSSDS 180

Query: 181 ----------------SVSG-----EEDIDLSPPRQRRKRYHTPSPESDVKPARSVSPQS 240
                           + SG     +EDIDLSPPRQRRKRYHTPSPESD KP +  SPQS
Sbjct: 181 SPPRKQNVYRGTHLQGADSGHLDHAQEDIDLSPPRQRRKRYHTPSPESDGKPTQPFSPQS 240

Query: 241 DLSPPRRSDRQASKANLGGNHKAAGLSDLSPPRRRTSGYADGAHISHESDLSPPRKQSKD 300
           D+SPPRR DRQASK++LG NHKAAGLSDLSPPRRRTS YA+ A++S  SDLSPPRKQ  D
Sbjct: 241 DMSPPRRVDRQASKSSLGVNHKAAGLSDLSPPRRRTSNYAEDAYVSRGSDLSPPRKQRID 300

Query: 301 VRRDRSLSDKHLQSHIVTDDSQESPADLSPHRKMQKALPVSVSFKQARKTGLLTQKEFGE 360
           VR DR L++ + Q+H+VTD SQESP DLSP RK QK  PVSVSFKQ RKTGLLTQ+EFGE
Sbjct: 301 VRGDRLLTENNSQNHVVTDASQESPPDLSPQRKKQKVPPVSVSFKQPRKTGLLTQQEFGE 360

Query: 361 EMSKTNKEDWTRFKEMNPSASSNAEPVYRDKIK-ERYRRSSFKRGILKSTRKNRRKAQGD 420
           EMSKTNKEDW RFKEMNPSASSNAEP+YRDKIK +R  +  F    LKS  K   K    
Sbjct: 361 EMSKTNKEDWRRFKEMNPSASSNAEPIYRDKIKGDRISKEEF----LKSRGKIEEK---- 420

Query: 421 KVLTITYMLLSNIIVAACDDLPSQEIKLEWGKGLAQKREAEAERMELELEKDRPFARSSN 480
                                  +EIKLEWGKGLAQKREAEAE+MELELEKDRPFARS N
Sbjct: 421 ----------------------PKEIKLEWGKGLAQKREAEAEQMELELEKDRPFARSRN 480

Query: 481 DAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRG 506
           DAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRG
Sbjct: 481 DAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRG 540

BLAST of Carg25483 vs. ExPASy TrEMBL
Match: A0A6J1DPM1 (BUD13 homolog OS=Momordica charantia OX=3673 GN=LOC111022658 PE=3 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 1.9e-196
Identity = 405/596 (67.95%), Postives = 430/596 (72.15%), Query Frame = 0

Query: 1   MSA-KSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNAL------------------ 60
           MSA KS SLKEYLKRYESNTE DKKKKKKKK+T  ATKPNAL                  
Sbjct: 2   MSANKSNSLKEYLKRYESNTEEDKKKKKKKKKTTVATKPNALGVLVVDQDPVWQKPIIIE 61

Query: 61  --------DEEPQVDEDIEVTRMRRLEELKAKRPYNSISEDGSGWVSLSPNHANSSMVNG 120
                   DEEPQVDEDIEV RMRRLEELKAKRPYNSISEDGSGWVSLSPN ANSS VN 
Sbjct: 62  EDNADNSTDEEPQVDEDIEVKRMRRLEELKAKRPYNSISEDGSGWVSLSPNRANSSTVNS 121

Query: 121 DLSPPRRTRARNDTPSPSNELKPSVSGE-------------------------------- 180
           D+SPPRR RARNDTPSP NEL+P VSGE                                
Sbjct: 122 DISPPRRRRARNDTPSPRNELEPPVSGEEGNDISPVWRRQGQHHTSLEHDEKPTSSAYPS 181

Query: 181 -----------------------------EDIDLSPPRQRRKRYHTPSPESDVK-PARSV 240
                                        EDIDLSPPRQRRKRYHTPSPE D K   RSV
Sbjct: 182 SDSSPPRKQKVYRDSPLQGANSGHLDSAQEDIDLSPPRQRRKRYHTPSPEPDEKQTVRSV 241

Query: 241 SPQSDLSPPRRSDRQASKANLGGNHKAAGLSDLSPPRRRTSGYADGAHISHESDLSPPRK 300
           SPQSD+SPPRRSDR  SKA+LGGNHKAAGLSDLSPPRRRTS YAD AHIS  SDLSPPRK
Sbjct: 242 SPQSDISPPRRSDRHTSKASLGGNHKAAGLSDLSPPRRRTSDYADDAHISRGSDLSPPRK 301

Query: 301 QSKDVRRDRSLSDKHLQSHIVTDDSQES-PADLSPHRKMQKALPVSVSFKQARKTGLLTQ 360
           Q KDVR ++SLSD+H ++ +VTD S+ES P+D+SP R+ QK LPVSVSFKQ RKTGL+TQ
Sbjct: 302 QRKDVRGNQSLSDQHSRNRVVTDASRESLPSDVSPRREKQKPLPVSVSFKQPRKTGLITQ 361

Query: 361 KEFGEEMSKTNKEDWTRFKEMNPSASSNAEPVYRDKIK-ERYRRSSFKRGILKSTRKNRR 420
           +EFGEEMSKTNKEDWTRFKEMNPSASSNAEPVYRD+IK +R  +  F    LKS  K   
Sbjct: 362 QEFGEEMSKTNKEDWTRFKEMNPSASSNAEPVYRDRIKGDRISKEEF----LKSRGKIEE 421

Query: 421 KAQGDKVLTITYMLLSNIIVAACDDLPSQEIKLEWGKGLAQKREAEAERMELELEKDRPF 480
           K                           +EIKLEWGKGLAQKREAEAE MELELEKDRPF
Sbjct: 422 K--------------------------PKEIKLEWGKGLAQKREAEAELMELELEKDRPF 481

Query: 481 ARSSNDAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHS 506
           ARS NDAELDSMLR+R+RWGDPMAHLVKKK+SEMAL DLGDSEKM+ESGFIIPQDIP HS
Sbjct: 482 ARSRNDAELDSMLRDRVRWGDPMAHLVKKKKSEMALLDLGDSEKMRESGFIIPQDIPAHS 541

BLAST of Carg25483 vs. ExPASy TrEMBL
Match: A0A1S3CHR8 (BUD13 homolog OS=Cucumis melo OX=3656 GN=LOC103501110 PE=3 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 1.2e-195
Identity = 400/591 (67.68%), Postives = 420/591 (71.07%), Query Frame = 0

Query: 1   MSAKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNAL------------------- 60
           MSAKSKSL+EYLKRYESNTE +KKKKKKKKRT    KPNAL                   
Sbjct: 2   MSAKSKSLQEYLKRYESNTEEEKKKKKKKKRTTATNKPNALGVLVVDEDPVWQKPIIIEE 61

Query: 61  -------DEEPQVDEDIEVTRMRRLEELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGD 120
                  DEEPQVDED+EV RMRRLEELKAKRPYNSISEDGSGWVSLS N ANSSM+N D
Sbjct: 62  DNADNSTDEEPQVDEDVEVKRMRRLEELKAKRPYNSISEDGSGWVSLSSNRANSSMMNSD 121

Query: 121 LSPPRRTRARNDTPSPSNELKPSVSGE--------------------------------- 180
           +SPPRRTR RNDTPSPSNELKP   GE                                 
Sbjct: 122 MSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNSTYPSS 181

Query: 181 --------------------------EDIDLSPPRQRRKRYHTPSPESDVKPARSVSPQS 240
                                     EDIDLSPPRQRRKRYHTPSPE +    RSVSPQS
Sbjct: 182 SPPQKDKVYRDAHLQGANWGHMDCAQEDIDLSPPRQRRKRYHTPSPEPEATRTRSVSPQS 241

Query: 241 DLSPPRRSDRQASKANLGGNHKAAGLSDLSPPRRRTSGYADGAHISHESDLSPPRKQSKD 300
           D+SPPRRSDR+ASKA+LGGNHK     DLSPPRRR S Y    HIS  SDLSPPRKQ KD
Sbjct: 242 DMSPPRRSDRKASKASLGGNHKV----DLSPPRRRASDYVGDEHISRGSDLSPPRKQRKD 301

Query: 301 VRRDRSLSDKHLQSHIVTDDSQESPADLSPHRKMQKALPVSVSFKQARKTGLLTQKEFGE 360
           VR DRSL DKH Q+H VTD SQES  DLSPHRK QK LPVSVSFKQ RKTGLLTQ+EFGE
Sbjct: 302 VRGDRSLLDKHSQNH-VTDASQESRTDLSPHRKKQKELPVSVSFKQTRKTGLLTQQEFGE 361

Query: 361 EMSKTNKEDWTRFKEMNPSASSNAEPVYRDKIK-ERYRRSSFKRGILKSTRKNRRKAQGD 420
           EMSKTNKEDWTRFKEMNPSASSNA+PVYRDKIK +R  +  F    LK+  K   K    
Sbjct: 362 EMSKTNKEDWTRFKEMNPSASSNADPVYRDKIKGDRISKEEF----LKARGKIEEK---- 421

Query: 421 KVLTITYMLLSNIIVAACDDLPSQEIKLEWGKGLAQKREAEAERMELELEKDRPFARSSN 480
                                  +EIKLEWGKGLAQKREAEAE MELELEKDRPFARS N
Sbjct: 422 ----------------------PKEIKLEWGKGLAQKREAEAELMELELEKDRPFARSRN 481

Query: 481 DAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRG 506
           DAELDSMLR+RLRWGDPMAHLVKK+QSEMALPDLGD+EKMKESGF+IPQDIPPHSWLKRG
Sbjct: 482 DAELDSMLRDRLRWGDPMAHLVKKRQSEMALPDLGDNEKMKESGFMIPQDIPPHSWLKRG 541

BLAST of Carg25483 vs. ExPASy TrEMBL
Match: A0A5A7VKV1 (BUD13-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G00590 PE=3 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 1.2e-195
Identity = 400/591 (67.68%), Postives = 420/591 (71.07%), Query Frame = 0

Query: 1   MSAKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKPNAL------------------- 60
           MSAKSKSL+EYLKRYESNTE +KKKKKKKKRT    KPNAL                   
Sbjct: 2   MSAKSKSLQEYLKRYESNTEEEKKKKKKKKRTTATNKPNALGVLVVDEDPVWQKPIIIEE 61

Query: 61  -------DEEPQVDEDIEVTRMRRLEELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGD 120
                  DEEPQVDED+EV RMRRLEELKAKRPYNSISEDGSGWVSLS N ANSSM+N D
Sbjct: 62  DNADNSTDEEPQVDEDVEVKRMRRLEELKAKRPYNSISEDGSGWVSLSSNRANSSMMNSD 121

Query: 121 LSPPRRTRARNDTPSPSNELKPSVSGE--------------------------------- 180
           +SPPRRTR RNDTPSPSNELKP   GE                                 
Sbjct: 122 MSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNSTYPSS 181

Query: 181 --------------------------EDIDLSPPRQRRKRYHTPSPESDVKPARSVSPQS 240
                                     EDIDLSPPRQRRKRYHTPSPE +    RSVSPQS
Sbjct: 182 SPPQKDKVYRDAHLQGANWGHMDCAQEDIDLSPPRQRRKRYHTPSPEPEATRTRSVSPQS 241

Query: 241 DLSPPRRSDRQASKANLGGNHKAAGLSDLSPPRRRTSGYADGAHISHESDLSPPRKQSKD 300
           D+SPPRRSDR+ASKA+LGGNHK     DLSPPRRR S Y    HIS  SDLSPPRKQ KD
Sbjct: 242 DMSPPRRSDRKASKASLGGNHKV----DLSPPRRRASDYVGDEHISRGSDLSPPRKQRKD 301

Query: 301 VRRDRSLSDKHLQSHIVTDDSQESPADLSPHRKMQKALPVSVSFKQARKTGLLTQKEFGE 360
           VR DRSL DKH Q+H VTD SQES  DLSPHRK QK LPVSVSFKQ RKTGLLTQ+EFGE
Sbjct: 302 VRGDRSLLDKHSQNH-VTDASQESRTDLSPHRKKQKELPVSVSFKQTRKTGLLTQQEFGE 361

Query: 361 EMSKTNKEDWTRFKEMNPSASSNAEPVYRDKIK-ERYRRSSFKRGILKSTRKNRRKAQGD 420
           EMSKTNKEDWTRFKEMNPSASSNA+PVYRDKIK +R  +  F    LK+  K   K    
Sbjct: 362 EMSKTNKEDWTRFKEMNPSASSNADPVYRDKIKGDRISKEEF----LKARGKIEEK---- 421

Query: 421 KVLTITYMLLSNIIVAACDDLPSQEIKLEWGKGLAQKREAEAERMELELEKDRPFARSSN 480
                                  +EIKLEWGKGLAQKREAEAE MELELEKDRPFARS N
Sbjct: 422 ----------------------PKEIKLEWGKGLAQKREAEAELMELELEKDRPFARSRN 481

Query: 481 DAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRG 506
           DAELDSMLR+RLRWGDPMAHLVKK+QSEMALPDLGD+EKMKESGF+IPQDIPPHSWLKRG
Sbjct: 482 DAELDSMLRDRLRWGDPMAHLVKKRQSEMALPDLGDNEKMKESGFMIPQDIPPHSWLKRG 541

BLAST of Carg25483 vs. TAIR 10
Match: AT1G31870.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cytosol, nucleus; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2050, pre-mRNA-splicing factor (InterPro:IPR018609); Has 13303 Blast hits to 9618 proteins in 489 species: Archae - 8; Bacteria - 444; Metazoa - 7153; Fungi - 2294; Plants - 942; Viruses - 128; Other Eukaryotes - 2334 (source: NCBI BLink). )

HSP 1 Score: 369.0 bits (946), Expect = 6.1e-102
Identity = 262/603 (43.45%), Postives = 342/603 (56.72%), Query Frame = 0

Query: 3   AKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKP------------------------ 62
           A ++SLK+YLK+YES+  V+KKKKKKK++  +  +P                        
Sbjct: 2   AGNQSLKDYLKKYESSDVVEKKKKKKKQKKPSKPEPRGVLVVDEDPVWQKQVDPEEDENE 61

Query: 63  -NALDEEPQVDEDIEVTRMRRLEELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGDLSP 122
            ++ +E P VDEDIEV RMRRLEE+KA+R +N+I+EDGSGWV+L  N  ++     ++SP
Sbjct: 62  DDSAEETPLVDEDIEVKRMRRLEEIKARRAHNAIAEDGSGWVTLPLNREDT---QSNISP 121

Query: 123 PRRTRARNDTPSPSNELKPSVSGEEDIDLSPPRQRRKRYHTPSPESDVKPARSVSPQSDL 182
           PRR R RND+PSP    + SV+   D D+SPPR RRKR+++PSPE + K  + VS  SD+
Sbjct: 122 PRRQRTRNDSPSPEPGPRRSVADRVDTDMSPPR-RRKRHNSPSPEPNRKHTKPVSLDSDM 181

Query: 183 SPPRRSDRQASKANLGGNHKAAGLS-DLSPPRR-------RTSGYADGAHISHESDLSPP 242
           SPPR+  R+A   +     +A  LS DLSPPRR       R S       +  + DLSPP
Sbjct: 182 SPPRK--RKARNDSPSPEPEAKYLSEDLSPPRRRHVHSPSRESSRKRSDSVELDDDLSPP 241

Query: 243 R--------------KQSKDV-----RRDRSLSDKHLQ----------------SHIVTD 302
           R              K+S D+     RR  S S +  +                 +I   
Sbjct: 242 RRKRDLHGSPVSDVKKKSNDLSPPRRRRYHSPSPEPARRSSKSFGSNADLSPPGRNINMK 301

Query: 303 DSQESPADLSPHRK-------------------------MQKALPVSVSFKQARKTGLLT 362
           DSQ+S  DLSP RK                          + + P  +S K+ RKTGL++
Sbjct: 302 DSQDS--DLSPQRKAADLRRSSENVSRSSNFDSSPPRRPRRDSSPPQIS-KEQRKTGLIS 361

Query: 363 QKEFGEEMSKTNKEDWTRFKEMNPS-ASSNAEPVYRDKI------KERYRRSSFKRGILK 422
            K+ G E  K  +++  RFK M+      NAE V+RDKI      KE Y +S  K+ I K
Sbjct: 362 GKDIGSEYRKKKEDEKLRFKNMDSELTGQNAEAVFRDKITGKRISKEEYLKSKQKKVIEK 421

Query: 423 STRKNRRKAQGDKVLTITYMLLSNIIVAACDDLPSQEIKLEWGKGLAQKREAEAERMELE 482
                                              +EIKLEWGKGLAQKREAEA   ELE
Sbjct: 422 ----------------------------------PKEIKLEWGKGLAQKREAEARLQELE 481

Query: 483 LEKDRPFARSSNDAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIP 506
           LEKD+PFAR+ +D ELD M++ER+R+GDPMAHLVKK++ E  L DLGD E+MK+SGFIIP
Sbjct: 482 LEKDKPFARTRDDPELDQMMKERVRFGDPMAHLVKKRKYETTLVDLGDDEEMKKSGFIIP 541

BLAST of Carg25483 vs. TAIR 10
Match: AT1G31870.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cytosol, nucleus; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2050, pre-mRNA-splicing factor (InterPro:IPR018609); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 369.0 bits (946), Expect = 6.1e-102
Identity = 262/603 (43.45%), Postives = 342/603 (56.72%), Query Frame = 0

Query: 3   AKSKSLKEYLKRYESNTEVDKKKKKKKKRTATATKP------------------------ 62
           A ++SLK+YLK+YES+  V+KKKKKKK++  +  +P                        
Sbjct: 2   AGNQSLKDYLKKYESSDVVEKKKKKKKQKKPSKPEPRGVLVVDEDPVWQKQVDPEEDENE 61

Query: 63  -NALDEEPQVDEDIEVTRMRRLEELKAKRPYNSISEDGSGWVSLSPNHANSSMVNGDLSP 122
            ++ +E P VDEDIEV RMRRLEE+KA+R +N+I+EDGSGWV+L  N  ++     ++SP
Sbjct: 62  DDSAEETPLVDEDIEVKRMRRLEEIKARRAHNAIAEDGSGWVTLPLNREDT---QSNISP 121

Query: 123 PRRTRARNDTPSPSNELKPSVSGEEDIDLSPPRQRRKRYHTPSPESDVKPARSVSPQSDL 182
           PRR R RND+PSP    + SV+   D D+SPPR RRKR+++PSPE + K  + VS  SD+
Sbjct: 122 PRRQRTRNDSPSPEPGPRRSVADRVDTDMSPPR-RRKRHNSPSPEPNRKHTKPVSLDSDM 181

Query: 183 SPPRRSDRQASKANLGGNHKAAGLS-DLSPPRR-------RTSGYADGAHISHESDLSPP 242
           SPPR+  R+A   +     +A  LS DLSPPRR       R S       +  + DLSPP
Sbjct: 182 SPPRK--RKARNDSPSPEPEAKYLSEDLSPPRRRHVHSPSRESSRKRSDSVELDDDLSPP 241

Query: 243 R--------------KQSKDV-----RRDRSLSDKHLQ----------------SHIVTD 302
           R              K+S D+     RR  S S +  +                 +I   
Sbjct: 242 RRKRDLHGSPVSDVKKKSNDLSPPRRRRYHSPSPEPARRSSKSFGSNADLSPPGRNINMK 301

Query: 303 DSQESPADLSPHRK-------------------------MQKALPVSVSFKQARKTGLLT 362
           DSQ+S  DLSP RK                          + + P  +S K+ RKTGL++
Sbjct: 302 DSQDS--DLSPQRKAADLRRSSENVSRSSNFDSSPPRRPRRDSSPPQIS-KEQRKTGLIS 361

Query: 363 QKEFGEEMSKTNKEDWTRFKEMNPS-ASSNAEPVYRDKI------KERYRRSSFKRGILK 422
            K+ G E  K  +++  RFK M+      NAE V+RDKI      KE Y +S  K+ I K
Sbjct: 362 GKDIGSEYRKKKEDEKLRFKNMDSELTGQNAEAVFRDKITGKRISKEEYLKSKQKKVIEK 421

Query: 423 STRKNRRKAQGDKVLTITYMLLSNIIVAACDDLPSQEIKLEWGKGLAQKREAEAERMELE 482
                                              +EIKLEWGKGLAQKREAEA   ELE
Sbjct: 422 ----------------------------------PKEIKLEWGKGLAQKREAEARLQELE 481

Query: 483 LEKDRPFARSSNDAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLGDSEKMKESGFIIP 506
           LEKD+PFAR+ +D ELD M++ER+R+GDPMAHLVKK++ E  L DLGD E+MK+SGFIIP
Sbjct: 482 LEKDKPFARTRDDPELDQMMKERVRFGDPMAHLVKKRKYETTLVDLGDDEEMKKSGFIIP 541