Carg25321 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25321
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Cytochrome c oxidase subunit 3
Location	Carg_Chr11: 10310353 .. 10311526 (-)
RNA-Seq Expression	Carg25321
Synteny	Carg25321

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCGTATGGTGGCGCGATGTTCTACGTGAATCCACGTTGGAAGGACATCATACCAAAGTCGTACAATTAGGACTTCGATATGGGTTTATTCTGTTTATCGTATCGGAGGTGATGTTCCTTTTTGCTTTTTTTCGGGCTTCTTCTCATTCTTCTTTGGCACCCACAGTAGAGATCGGAGGTATTTGGCCCCCAAAAGGGATTGGGGTTTTAGATCCTCGGGAATCATATTTCTTTTCTTAATAATTCTGATTCATCTGTTCCAGCCGGAGCAGCCAACATTCGGGACTCATCATGCTATATTCGCGCGGGGAATAGTGATAAATCGAGCAGTGTGATACGCTTTATAGTAGCTACCGTTTCACTGGCTGAACGTATCAACAGGCTTTCAAAGTATTGGAAATAATTAATGCAAGCACCCTTTTATATCTAGATTGGTTGATAGTATTATATGGGTTTCTCTAATCGCGCCTTCTTCTTATGCACCTGGCTTTCATGGGTTTCATGTGATTATGGTACTCTTTTCTTGATCATATGTAGAGTAACCTTATCGCAAATAATTTTGTCTATACTAACCAAAAGAGTGCAGTCACGCTTGGCTTTGAAGCACAGCTGCATGGTAACTGGATTGCAATTTCTGTAGACGTGGATTCGGTTTATTCTCTCACTTGGTCTCTATAGATTGGTGGAGGAGTATATGAAAGAACAAAAGCGTGCATTGAGGATTCAACTCGGAAGACATCAGAGAATCGGTGCTTTCCAAAGAATTCCAGTAGCTTTTCCTTCTTCCTTTAATTATTATATAGAAAAAGTGCTCTTCCGTATCTTGTTCCCTACCAATCTATCTGTATTCTGGCACATAAATTCAGGGATCGAAGAGATTATGGCAGATCATGTTCACCAAGAAATGACCCGAAATTGGATCTTAGTCTATTTGAGATTGTTCCTTTTAATCGTAATCAAAGATGTTTTCTTGTCTCTCATTTCTTTTCCGAATAAATCGAAGAACCTAATGGATCGAACGAACTCATCCTTGGCAGCTATGAAAGATATCGCCCTTGCGGAAGGAAGATTCTCTTATCTTTTGAGTTTCAGGCAAGAGCTCTTTTTCAATAAAGGGTTGGAACTCTCATCTTTACGGGAAGAATATGGTATGGGCTTGGTGAAGCTATGA

mRNA sequence

ATGTTCGTATGGTGGCGCGATGTTCTACGTGAATCCACGTTGGAAGGACATCATACCAAAGTCGTACAATTAGGACTTCGATATGGGTTTATTCTGTTTATCGTATCGGAGGTGATGTTCCTTTTTGCTTTTTTTCGGGCTTCTTCTCATTCTTCTTTGGCACCCACAGTAGAGATCGGAGCCGGAGCAGCCAACATTCGGGACTCATCATGCTATATTCGCGCGGGGAATAGTGATAAATCGAGCAGTGTGATACGCTTTATAGTAGCTACCGTTTCACTGGCTGAACGTATCAACAGGCTTTCAAAATTGGTTGATAGTATTATATGGGTTTCTCTAATCGCGCCTTCTTCTTATGCACCTGGCTTTCATGGGTTTCATGTGATTATGACGTGGATTCGGTTTATTCTCTCACTTGGTCTCTATAGATTGGTGGAGGAGTATATGAAAGAACAAAAGCGTGCATTGAGGATTCAACTCGGAAGACATCAGAGAATCGGTGCTTTCCAAAGAATTCCAGTAGCTTTTCCTTCTTCCTTTAATTATTATATAGAAAAAGTGCTCTTCCGTATCTTGTTCCCTACCAATCTATCTGTATTCTGGCACATAAATTCAGGGATCGAAGAGATTATGGCAGATCATGTTCACCAAGAAATGACCCGAAATTGGATCTTAGTCTATTTGAGATTGTTCCTTTTAATCGTAATCAAAGATGTTTTCTTGTCTCTCATTTCTTTTCCGAATAAATCGAAGAACCTAATGGATCGAACGAACTCATCCTTGGCAGCTATGAAAGATATCGCCCTTGCGGAAGGAAGATTCTCTTATCTTTTGAGTTTCAGGCAAGAGCTCTTTTTCAATAAAGGGTTGGAACTCTCATCTTTACGGGAAGAATATGGTATGGGCTTGGTGAAGCTATGA

Coding sequence (CDS)

ATGTTCGTATGGTGGCGCGATGTTCTACGTGAATCCACGTTGGAAGGACATCATACCAAAGTCGTACAATTAGGACTTCGATATGGGTTTATTCTGTTTATCGTATCGGAGGTGATGTTCCTTTTTGCTTTTTTTCGGGCTTCTTCTCATTCTTCTTTGGCACCCACAGTAGAGATCGGAGCCGGAGCAGCCAACATTCGGGACTCATCATGCTATATTCGCGCGGGGAATAGTGATAAATCGAGCAGTGTGATACGCTTTATAGTAGCTACCGTTTCACTGGCTGAACGTATCAACAGGCTTTCAAAATTGGTTGATAGTATTATATGGGTTTCTCTAATCGCGCCTTCTTCTTATGCACCTGGCTTTCATGGGTTTCATGTGATTATGACGTGGATTCGGTTTATTCTCTCACTTGGTCTCTATAGATTGGTGGAGGAGTATATGAAAGAACAAAAGCGTGCATTGAGGATTCAACTCGGAAGACATCAGAGAATCGGTGCTTTCCAAAGAATTCCAGTAGCTTTTCCTTCTTCCTTTAATTATTATATAGAAAAAGTGCTCTTCCGTATCTTGTTCCCTACCAATCTATCTGTATTCTGGCACATAAATTCAGGGATCGAAGAGATTATGGCAGATCATGTTCACCAAGAAATGACCCGAAATTGGATCTTAGTCTATTTGAGATTGTTCCTTTTAATCGTAATCAAAGATGTTTTCTTGTCTCTCATTTCTTTTCCGAATAAATCGAAGAACCTAATGGATCGAACGAACTCATCCTTGGCAGCTATGAAAGATATCGCCCTTGCGGAAGGAAGATTCTCTTATCTTTTGAGTTTCAGGCAAGAGCTCTTTTTCAATAAAGGGTTGGAACTCTCATCTTTACGGGAAGAATATGGTATGGGCTTGGTGAAGCTATGA

Protein sequence

MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIGAGAANIRDSSCYIRAGNSDKSSSVIRFIVATVSLAERINRLSKLVDSIIWVSLIAPSSYAPGFHGFHVIMTWIRFILSLGLYRLVEEYMKEQKRALRIQLGRHQRIGAFQRIPVAFPSSFNYYIEKVLFRILFPTNLSVFWHINSGIEEIMADHVHQEMTRNWILVYLRLFLLIVIKDVFLSLISFPNKSKNLMDRTNSSLAAMKDIALAEGRFSYLLSFRQELFFNKGLELSSLREEYGMGLVKL

Homology

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BLAST of Carg25321 vs. NCBI nr
Match: KAG7022597.1 (Cytochrome c oxidase subunit 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 592.8 bits (1527), Expect = 1.7e-165
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG
Sbjct: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60

Query: 61  AGAANIRDSSCYIRAGNSDKSSSVIRFIVATVSLAERINRLSKLVDSIIWVSLIAPSSYA 120
           AGAANIRDSSCYIRAGNSDKSSSVIRFIVATVSLAERINRLSKLVDSIIWVSLIAPSSYA
Sbjct: 61  AGAANIRDSSCYIRAGNSDKSSSVIRFIVATVSLAERINRLSKLVDSIIWVSLIAPSSYA 120

Query: 121 PGFHGFHVIMTWIRFILSLGLYRLVEEYMKEQKRALRIQLGRHQRIGAFQRIPVAFPSSF 180
           PGFHGFHVIMTWIRFILSLGLYRLVEEYMKEQKRALRIQLGRHQRIGAFQRIPVAFPSSF
Sbjct: 121 PGFHGFHVIMTWIRFILSLGLYRLVEEYMKEQKRALRIQLGRHQRIGAFQRIPVAFPSSF 180

Query: 181 NYYIEKVLFRILFPTNLSVFWHINSGIEEIMADHVHQEMTRNWILVYLRLFLLIVIKDVF 240
           NYYIEKVLFRILFPTNLSVFWHINSGIEEIMADHVHQEMTRNWILVYLRLFLLIVIKDVF
Sbjct: 181 NYYIEKVLFRILFPTNLSVFWHINSGIEEIMADHVHQEMTRNWILVYLRLFLLIVIKDVF 240

Query: 241 LSLISFPNKSKNLMDRTNSSLAAMKDIALAEGRFSYLLSFRQELFFNKGLELSSLREEYG 300
           LSLISFPNKSKNLMDRTNSSLAAMKDIALAEGRFSYLLSFRQELFFNKGLELSSLREEYG
Sbjct: 241 LSLISFPNKSKNLMDRTNSSLAAMKDIALAEGRFSYLLSFRQELFFNKGLELSSLREEYG 300

Query: 301 MGLVKL 307
           MGLVKL
Sbjct: 301 MGLVKL 306

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BLAST of Carg25321 vs. NCBI nr
Match: KAE9584275.1 (putative cytochrome-c oxidase [Lupinus albus])

HSP 1 Score: 228.0 bits (580), Expect = 1.1e-55
Identity = 153/307 (49.84%), Postives = 181/307 (58.96%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTKVVQLG RYG I FIVSEVMFLFAFFRASSHSSLAPTVEIG
Sbjct: 57  MFVWWRDVLRESTLEGHHTKVVQLGPRYGSIPFIVSEVMFLFAFFRASSHSSLAPTVEIG 116

Query: 61  A-------GAANIRD-----SSCYIRAGN----------SDKSSSVIRFIVATVSLA--- 120
                   G  + R+     +   + +G           + K    +  +VATVSLA   
Sbjct: 117 GIWPPKGIGVLDPREIPFLNTPILLSSGAAVTWAHHAILAGKEKRAVYALVATVSLALVF 176

Query: 121 ------ERINRLSKLVDSIIWVSLIAPSSYAPGFHGFHVIMTWIRFILSLGLYRLVEEYM 180
                 E       + DSI   +       A GFHGFHVI+  + F++  G+ + +    
Sbjct: 177 TGFQGMEYYQAPFTISDSIYGSTFF----LATGFHGFHVIIGTL-FLIICGIRQYLGHLT 236

Query: 181 K------------------EQKRALRIQLGRHQRIGAFQRIPVAFPSSFNYYIEKVLFRI 240
           K                  E+K  LR +  + +R G FQRI  AFP       +KV   I
Sbjct: 237 KEHHVGFEAAAWYWHFVDVERKSGLRNESSKTKRTGLFQRITAAFPLPLILIYKKVSSTI 296

Query: 241 LFPTNLSVFWHINSGIEEIMADHVHQEMTRNWILVYLRLFLLIVIKDVFLSLISFPNKSK 259
           L   NLS+ WHIN GIEEI++D+VHQEMTRNWILVYLR F LIVIKDVFLSL+SF NKSK
Sbjct: 297 L--PNLSLLWHINEGIEEIISDYVHQEMTRNWILVYLRFFFLIVIKDVFLSLVSFLNKSK 356

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BLAST of Carg25321 vs. NCBI nr
Match: KAE8648258.1 (hypothetical protein Csa_018513 [Cucumis sativus])

HSP 1 Score: 218.0 bits (554), Expect = 1.1e-52
Identity = 153/305 (50.16%), Postives = 182/305 (59.67%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG
Sbjct: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60

Query: 61  A-------GAANIRD-----SSCYIRAGN----------SDKSSSVIRFIVATVSLA--- 120
                   G  + R+     +   + +G           + K   V+  +VATVSLA   
Sbjct: 61  GIWPPKGIGVLDPREIPFLNTPILLSSGAAVTWAHHAILAGKEKRVVYALVATVSLALVF 120

Query: 121 ------ERINRLSKLVDSIIWVSLIAPSSYAPGFHGFHVIMTWIRFILSLGLYRLVEEYM 180
                 E       + DSI   +    +S    FHGFHVI+  + F++  G+ + +    
Sbjct: 121 TSFQGMEYYQAPFTISDSIYGSTFFLATS----FHGFHVIIGTL-FLIVCGIRQYLGHLT 180

Query: 181 KEQKRALRIQLGRHQRIGAFQRIPVAF-PSSFNYYIEKVLFRILFPTNLSVFWHINSGIE 240
           KE                      V F  +++ ++   V+   LF   +S++W    GIE
Sbjct: 181 KEHH--------------------VGFEAAAWYWHFVDVVRLFLF---VSIYWW--GGIE 240

Query: 241 EIMADHVHQEMTRNWILVYLRLFLLIVIKDVFLSLISFPNKSKNLMDRTNSSLAAMKDIA 274
           EIMADHVHQEMTRNWILVYLR FL I IKD+FLSLISFPN+SKNLMD   SSL AMKDI 
Sbjct: 241 EIMADHVHQEMTRNWILVYLRFFLFISIKDLFLSLISFPNQSKNLMD---SSLPAMKDIG 272

[Close]

BLAST of Carg25321 vs. NCBI nr
Match: TYJ97776.1 (cytochrome c oxidase subunit 3 [Cucumis melo var. makuwa])

HSP 1 Score: 210.3 bits (534), Expect = 2.3e-50
Identity = 148/303 (48.84%), Postives = 179/303 (59.08%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTKVVQLGLRYGF+LFIVSEVMFLFAFFRASSHSSLAPTVEIG
Sbjct: 57  MFVWWRDVLRESTLEGHHTKVVQLGLRYGFLLFIVSEVMFLFAFFRASSHSSLAPTVEIG 116

Query: 61  A-------GAANIRD-----SSCYIRAGN----------SDKSSSVIRFIVATVSLA--- 120
                   G  + R+     +   + +G           + K    +  +VATVSLA   
Sbjct: 117 GIWPPKGIGVLDPREIPFLNTPILLSSGAAVTWAHHAILAGKEKRAVYALVATVSLALVF 176

Query: 121 ------ERINRLSKLVDSIIWVSLIAPSSYAPGFHGFHVIMTWIRFILSLGLYRLVEEYM 180
                 E       + DSI   +       A GFHGFHVI+  + F++  G+ + +    
Sbjct: 177 TGFQGMEYYQAPFTISDSIYGSTFF----LATGFHGFHVIIGTL-FLIVCGIRQYLGHLT 236

Query: 181 KEQKRALRIQLGRHQRIGAFQRIPVAF-PSSFNYYIEKVLFRILFPTNLSVFWHINSGIE 240
           KE                      V F  +++ ++   V+   LF   +S++W    GIE
Sbjct: 237 KEHH--------------------VGFEAAAWYWHFVDVVRLFLF---VSIYWW--GGIE 296

Query: 241 EIMADHVHQEMTRNWILVYLRLFLLIVIKDVFLSLISFPNKSKNLMDRTNSSLAAMKDIA 272
           EIMADHVHQEMTRNWILVYLRLFL I IKD+FL+LISFPNKSKNLMD   SSL AMKDI 
Sbjct: 297 EIMADHVHQEMTRNWILVYLRLFLFISIKDLFLALISFPNKSKNLMD---SSLPAMKDIH 326

[Close]

BLAST of Carg25321 vs. NCBI nr
Match: GAY69439.1 (hypothetical protein CUMW_272010 [Citrus unshiu])

HSP 1 Score: 198.0 bits (502), Expect = 1.2e-46
Identity = 136/287 (47.39%), Postives = 150/287 (52.26%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVM  FAFF A  HSSLAPTVEIG
Sbjct: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMLFFAFFWAFFHSSLAPTVEIG 60

Query: 61  -------------------------AGAANIRDSSCYIRAGNSDKSSSVIRFIVATVSLA 120
                                    +  A +  +   I AG   K    +  +VATV LA
Sbjct: 61  GIWPPKGIWVLDPWEIPFLNTLILLSSGAAVTWAHHAILAG---KEKRAVYALVATVLLA 120

Query: 121 ERINRLSKL----VDSIIWVSLIAPSSY-APGFHGFHVIMTWIRFILSLGLYRLVEEYMK 180
                   +        I  S+   + Y A GFHGFHVI+    F+L             
Sbjct: 121 LVFTGFQGMEYYQAPFTISDSIYGSTFYLATGFHGFHVIIVSSTFLL------------- 180

Query: 181 EQKRALRIQLGRHQRIGAFQRIPVAFPSSFNYYIEKVLFRILFPTNLSVFWHINSGIEEI 240
                                                        NLS+FWHIN GIEEI
Sbjct: 181 ---------------------------------------------NLSLFWHINEGIEEI 226

Query: 241 MADHVHQEMTRNWILVYLRLFLLIVIKDVFLSLISFPNKSKNLMDRT 258
           MAD+VHQEMTRNWIL+YLRLFLLIVIKDVFLSL+SF NKSKNLMDRT
Sbjct: 241 MADYVHQEMTRNWILIYLRLFLLIVIKDVFLSLVSFLNKSKNLMDRT 226

[Close]

BLAST of Carg25321 vs. ExPASy Swiss-Prot
Match: P32808 (Cytochrome c oxidase subunit 3 OS=Helianthus annuus OX=4232 GN=COX3 PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 8.4e-27
Identity = 88/185 (47.57%), Postives = 101/185 (54.59%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTKVVQLG RYGFILFIVSEVMFLFA FRASSHSSLAPTVEIG
Sbjct: 57  MFVWWRDVLRESTLEGHHTKVVQLGPRYGFILFIVSEVMFLFALFRASSHSSLAPTVEIG 116

Query: 61  -------------------------AGAANIRDSSCYIRAGNSDKSSSVIRFIVATVSLA 120
                                    +  A +  +   I AG   K    +  +VATVSLA
Sbjct: 117 GIWPPKGIAVLDPREIPFLNTPIPLSSGAAVTWAHHAILAG---KEKRAVYALVATVSLA 176

Query: 121 ---------ERINRLSKLVDSIIWVSLIAPSSYAPGFHGFHVIMTWIRFILSLGLYRLVE 152
                    E     S + DSI   +       A GFHGFHVI+  + F +  G+ + + 
Sbjct: 177 LVFTAFQGMEYYQAPSTISDSIYGSTFF----LATGFHGFHVIIGTL-FSIVCGIRQYLG 233

[Close]

BLAST of Carg25321 vs. ExPASy Swiss-Prot
Match: P08745 (Cytochrome c oxidase subunit 3 OS=Oenothera berteroana OX=3950 GN=COX3 PE=3 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 5.5e-26
Identity = 86/181 (47.51%), Postives = 100/181 (55.25%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTKVVQLGLRYG ILFIVSEVMFLFAFF ASSHSSLAPTVEIG
Sbjct: 57  MFVWWRDVLRESTLEGHHTKVVQLGLRYGSILFIVSEVMFLFAFFWASSHSSLAPTVEIG 116

Query: 61  -------------------------AGAANIRDSSCYIRAGNSDKSSSVIRFIVATVSLA 120
                                    +  A +  +   I AG   K    +  +VATVSLA
Sbjct: 117 GIWPPKGIGVLDPWEIPFLNTPILLSSGAAVTWAHHAILAG---KEKRAVYALVATVSLA 176

Query: 121 ERINRLSKL----VDSIIWVSLIAPSSY-APGFHGFHVIMTWIRFILSLGLYRLVEEYMK 152
                   +        I  S+   + Y A GFHGFHVI+  + F +  G+ + +    K
Sbjct: 177 IVFTGFQGMEYYQAPPTISDSIYGSTFYLATGFHGFHVIIGTL-FSIICGIRQYLGHLTK 233

[Close]

BLAST of Carg25321 vs. ExPASy Swiss-Prot
Match: Q03227 (Cytochrome c oxidase subunit 3 OS=Vicia faba OX=3906 GN=COX3 PE=3 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 7.1e-26
Identity = 85/182 (46.70%), Postives = 103/182 (56.59%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTKVVQLG RYG I FIVSEVMFLFAFFRASSHSSLAPTVEIG
Sbjct: 57  MFVWWRDVLRESTLEGHHTKVVQLGPRYGSISFIVSEVMFLFAFFRASSHSSLAPTVEIG 116

Query: 61  A-------GAANIRD-----SSCYIRAGN----------SDKSSSVIRFIVATVSLA--- 120
                   G  + R+     +   + +G           + K    +  +VATVSLA   
Sbjct: 117 GIWPPKGIGVLDPREIPFLNTPILLSSGAAVTWAHHAILAGKEKRAVYALVATVSLALVF 176

Query: 121 ------ERINRLSKLVDSIIWVSLIAPSSYAPGFHGFHVIMTWIRFILSLGLYRLVEEYM 152
                 E       + DSI   +       A GFHGFHVI+  + F++  G+ + + +  
Sbjct: 177 TGFQGMEYYQAPFTISDSIYGSTFF----LATGFHGFHVIIGTL-FLIICGIRQYLGQMT 233

[Close]

BLAST of Carg25321 vs. ExPASy Swiss-Prot
Match: Q36952 (Cytochrome c oxidase subunit 3 OS=Aegilops columnaris OX=4493 GN=COX3 PE=3 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 3.5e-25
Identity = 84/175 (48.00%), Postives = 96/175 (54.86%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTK VQLG RYG ILFIVSEVMFLFAFF ASSHSSLAPTVEIG
Sbjct: 57  MFVWWRDVLRESTLEGHHTKAVQLGPRYGSILFIVSEVMFLFAFFWASSHSSLAPTVEIG 116

Query: 61  -------------------------AGAANIRDSSCYIRAGNSDKSSSVIRFIVATVSLA 120
                                    +  A +  +   I AG   K    +  +VATVSLA
Sbjct: 117 GIWPPKGIGVLDPWEIPLLNTPILPSSGAAVTWAHHAILAG---KEKRAVYALVATVSLA 176

Query: 121 ---------ERINRLSKLVDSIIWVSLIAPSSYAPGFHGFHVIMTWIRFILSLGL 142
                    E     S + DSI   +       A GFHGFHVI+  + F++  G+
Sbjct: 177 LVSTGFQGMEYYQAPSTISDSIYGSTFF----LATGFHGFHVIIGTL-FLIVCGI 223

[Close]

BLAST of Carg25321 vs. ExPASy Swiss-Prot
Match: P14853 (Cytochrome c oxidase subunit 3 OS=Glycine max OX=3847 GN=COX3 PE=2 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.3e-24
Identity = 85/185 (45.95%), Postives = 99/185 (53.51%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTKVVQLG RYG I FIVSEVMFLFAFF ASSHSSLAPTVEIG
Sbjct: 57  MFVWWRDVLRESTLEGHHTKVVQLGPRYGSIPFIVSEVMFLFAFFWASSHSSLAPTVEIG 116

Query: 61  -------------------------AGAANIRDSSCYIRAGNSDKSSSVIRFIVATVSLA 120
                                    +  A +  +   I AG   K    +  +VATVSLA
Sbjct: 117 GIWPPLGIWVLDPWEIPFLNTPILLSSGAAVTWAHHAILAG---KEKRAVYALVATVSLA 176

Query: 121 ---------ERINRLSKLVDSIIWVSLIAPSSYAPGFHGFHVIMTWIRFILSLGLYRLVE 152
                    E       + DSI   +       A GFHGFHVI+  + F++  G+ + + 
Sbjct: 177 LVFTGFQGMEYYQAPFTISDSIYGSTFF----LATGFHGFHVIIGTL-FLIICGIRQYLG 233

[Close]

BLAST of Carg25321 vs. ExPASy TrEMBL
Match: A0A6A4MVS0 (Cytochrome c oxidase subunit 3 OS=Lupinus albus OX=3870 GN=Lalb_Chr00c24g0406721 PE=3 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 5.3e-56
Identity = 153/307 (49.84%), Postives = 181/307 (58.96%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTKVVQLG RYG I FIVSEVMFLFAFFRASSHSSLAPTVEIG
Sbjct: 57  MFVWWRDVLRESTLEGHHTKVVQLGPRYGSIPFIVSEVMFLFAFFRASSHSSLAPTVEIG 116

Query: 61  A-------GAANIRD-----SSCYIRAGN----------SDKSSSVIRFIVATVSLA--- 120
                   G  + R+     +   + +G           + K    +  +VATVSLA   
Sbjct: 117 GIWPPKGIGVLDPREIPFLNTPILLSSGAAVTWAHHAILAGKEKRAVYALVATVSLALVF 176

Query: 121 ------ERINRLSKLVDSIIWVSLIAPSSYAPGFHGFHVIMTWIRFILSLGLYRLVEEYM 180
                 E       + DSI   +       A GFHGFHVI+  + F++  G+ + +    
Sbjct: 177 TGFQGMEYYQAPFTISDSIYGSTFF----LATGFHGFHVIIGTL-FLIICGIRQYLGHLT 236

Query: 181 K------------------EQKRALRIQLGRHQRIGAFQRIPVAFPSSFNYYIEKVLFRI 240
           K                  E+K  LR +  + +R G FQRI  AFP       +KV   I
Sbjct: 237 KEHHVGFEAAAWYWHFVDVERKSGLRNESSKTKRTGLFQRITAAFPLPLILIYKKVSSTI 296

Query: 241 LFPTNLSVFWHINSGIEEIMADHVHQEMTRNWILVYLRLFLLIVIKDVFLSLISFPNKSK 259
           L   NLS+ WHIN GIEEI++D+VHQEMTRNWILVYLR F LIVIKDVFLSL+SF NKSK
Sbjct: 297 L--PNLSLLWHINEGIEEIISDYVHQEMTRNWILVYLRFFFLIVIKDVFLSLVSFLNKSK 356

[Close]

BLAST of Carg25321 vs. ExPASy TrEMBL
Match: A0A6N2KUH8 (Cytochrome c oxidase subunit 3 OS=Salix viminalis OX=40686 GN=SVIM_LOCUS97736 PE=3 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 1.4e-53
Identity = 151/307 (49.19%), Postives = 173/307 (56.35%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMF FAFF A  HSSLAPTVEIG
Sbjct: 24  MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFFFAFFWAFFHSSLAPTVEIG 83

Query: 61  -------------------------AGAANIRDSSCYIRAGNSDKSSSVIRFIVATVSLA 120
                                    +  A +  +   I AG   K    +  + ATV LA
Sbjct: 84  GIWPPKGIGVLDPWEIPFLNTLILLSSGAAVTWAHHAILAG---KEKRAVYALAATVLLA 143

Query: 121 ---------ERINRLSKLVDSIIWVSLIAPSSYAPGFHGFHVIMTWIRFILSLGLYRLVE 180
                    E       + DSI   +       A GFHGFHVI+  + F++  G+ + + 
Sbjct: 144 LVFTGFQGMEYYQAPFTISDSIYGSTFF----LATGFHGFHVIIGTL-FLIICGIRQYLG 203

Query: 181 EYMK------------------EQKRALRIQLGRHQRIGAFQRIPVAFPSSFNYYIEKVL 240
              K                  E+K  LR +  + +RIG FQRI  A         +KV 
Sbjct: 204 HLTKEHHVGFEAAAWYWHFVDVERKSGLRNESSKTKRIGLFQRITAALLLPLIIIYKKV- 263

Query: 241 FRILFPTNLSVFWHINSGIEEIMADHVHQEMTRNWILVYLRLFLLIVIKDVFLSLISFPN 256
               F  NLS+FWHIN GIEEIMAD+VHQEMTRNWILVYLRLFLLIVIKDVFLS +SF N
Sbjct: 264 -SSTFLPNLSLFWHINEGIEEIMADYVHQEMTRNWILVYLRLFLLIVIKDVFLSFVSFLN 320

[Close]

BLAST of Carg25321 vs. ExPASy TrEMBL
Match: A0A6N2KDM9 (Cytochrome c oxidase subunit 3 OS=Salix viminalis OX=40686 GN=SVIM_LOCUS71748 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 4.6e-52
Identity = 153/319 (47.96%), Postives = 172/319 (53.92%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMF FAFF A  HSSLAPTVEIG
Sbjct: 24  MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFFFAFFWAFFHSSLAPTVEIG 83

Query: 61  -------------------------AGAANIRDSSCYIRAGNSDKSSSVIRFIVATVSLA 120
                                    +  A +  +   I AG   K    +  + ATV LA
Sbjct: 84  GIWPPKGIGVLDPWEIPFLNTLILLSSGAAVTWAHHAILAG---KEKRAVYALAATVLLA 143

Query: 121 ---------ERINRLSKLVDSIIWVSLIAPSSYAPGFHGFHVIM---------------- 180
                    E       + DSI   +       A GFHGFHVI+                
Sbjct: 144 LVFTGFQGMEYYQAPFTISDSIYGSTFF----LATGFHGFHVIIGTLFLIICGIRQYLGH 203

Query: 181 ------------TWI-RFILSLGLYRLVEEY-MKEQKRALRIQLGRHQRIGAFQRIPVAF 240
                        W   F+  + L+  V  Y   E+K  LR +  + +RIG FQRI  A 
Sbjct: 204 LTKEHHVGFEAAAWYWHFVDVVWLFLFVSIYWWGERKSGLRNESSKTKRIGLFQRITAAL 263

Query: 241 PSSFNYYIEKVLFRILFPTNLSVFWHINSGIEEIMADHVHQEMTRNWILVYLRLFLLIVI 256
                   +KV     F  NLS+FWHIN GIEEIMAD+VHQEMTRNWILVYLRLFLLIVI
Sbjct: 264 LLPLIIIYKKV--SSTFLPNLSLFWHINEGIEEIMADYVHQEMTRNWILVYLRLFLLIVI 323

[Close]

BLAST of Carg25321 vs. ExPASy TrEMBL
Match: A0A5D3BFB3 (Cytochrome c oxidase subunit 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold75799G00010 PE=3 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 1.1e-50
Identity = 148/303 (48.84%), Postives = 179/303 (59.08%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTKVVQLGLRYGF+LFIVSEVMFLFAFFRASSHSSLAPTVEIG
Sbjct: 57  MFVWWRDVLRESTLEGHHTKVVQLGLRYGFLLFIVSEVMFLFAFFRASSHSSLAPTVEIG 116

Query: 61  A-------GAANIRD-----SSCYIRAGN----------SDKSSSVIRFIVATVSLA--- 120
                   G  + R+     +   + +G           + K    +  +VATVSLA   
Sbjct: 117 GIWPPKGIGVLDPREIPFLNTPILLSSGAAVTWAHHAILAGKEKRAVYALVATVSLALVF 176

Query: 121 ------ERINRLSKLVDSIIWVSLIAPSSYAPGFHGFHVIMTWIRFILSLGLYRLVEEYM 180
                 E       + DSI   +       A GFHGFHVI+  + F++  G+ + +    
Sbjct: 177 TGFQGMEYYQAPFTISDSIYGSTFF----LATGFHGFHVIIGTL-FLIVCGIRQYLGHLT 236

Query: 181 KEQKRALRIQLGRHQRIGAFQRIPVAF-PSSFNYYIEKVLFRILFPTNLSVFWHINSGIE 240
           KE                      V F  +++ ++   V+   LF   +S++W    GIE
Sbjct: 237 KEHH--------------------VGFEAAAWYWHFVDVVRLFLF---VSIYWW--GGIE 296

Query: 241 EIMADHVHQEMTRNWILVYLRLFLLIVIKDVFLSLISFPNKSKNLMDRTNSSLAAMKDIA 272
           EIMADHVHQEMTRNWILVYLRLFL I IKD+FL+LISFPNKSKNLMD   SSL AMKDI 
Sbjct: 297 EIMADHVHQEMTRNWILVYLRLFLFISIKDLFLALISFPNKSKNLMD---SSLPAMKDIH 326

[Close]

BLAST of Carg25321 vs. ExPASy TrEMBL
Match: A0A2H5QXW9 (Cytochrome c oxidase subunit 3 OS=Citrus unshiu OX=55188 GN=CUMW_272010 PE=3 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 5.8e-47
Identity = 136/287 (47.39%), Postives = 150/287 (52.26%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVM  FAFF A  HSSLAPTVEIG
Sbjct: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMLFFAFFWAFFHSSLAPTVEIG 60

Query: 61  -------------------------AGAANIRDSSCYIRAGNSDKSSSVIRFIVATVSLA 120
                                    +  A +  +   I AG   K    +  +VATV LA
Sbjct: 61  GIWPPKGIWVLDPWEIPFLNTLILLSSGAAVTWAHHAILAG---KEKRAVYALVATVLLA 120

Query: 121 ERINRLSKL----VDSIIWVSLIAPSSY-APGFHGFHVIMTWIRFILSLGLYRLVEEYMK 180
                   +        I  S+   + Y A GFHGFHVI+    F+L             
Sbjct: 121 LVFTGFQGMEYYQAPFTISDSIYGSTFYLATGFHGFHVIIVSSTFLL------------- 180

Query: 181 EQKRALRIQLGRHQRIGAFQRIPVAFPSSFNYYIEKVLFRILFPTNLSVFWHINSGIEEI 240
                                                        NLS+FWHIN GIEEI
Sbjct: 181 ---------------------------------------------NLSLFWHINEGIEEI 226

Query: 241 MADHVHQEMTRNWILVYLRLFLLIVIKDVFLSLISFPNKSKNLMDRT 258
           MAD+VHQEMTRNWIL+YLRLFLLIVIKDVFLSL+SF NKSKNLMDRT
Sbjct: 241 MADYVHQEMTRNWILIYLRLFLLIVIKDVFLSLVSFLNKSKNLMDRT 226

[Close]

BLAST of Carg25321 vs. TAIR 10
Match: AT2G07687.1 (Cytochrome c oxidase, subunit III )

HSP 1 Score: 112.1 bits (279), Expect = 8.1e-25
Identity = 83/185 (44.86%), Postives = 97/185 (52.43%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTKVVQLG RYG ILFIVSEVMF FAFF ASSHSSLAP VEIG
Sbjct: 57  MFVWWRDVLRESTLEGHHTKVVQLGPRYGSILFIVSEVMFFFAFFWASSHSSLAPAVEIG 116

Query: 61  -------------------------AGAANIRDSSCYIRAGNSDKSSSVIRFIVATVSLA 120
                                    +  A +  +   I AG   K    +  +VATV LA
Sbjct: 117 GIWPPKGIEVLDPWEIPFLNTPILPSSGAAVTWAHHAILAG---KEKRAVYALVATVLLA 176

Query: 121 ---------ERINRLSKLVDSIIWVSLIAPSSYAPGFHGFHVIMTWIRFILSLGLYRLVE 152
                    E       + DSI   +       A GFHGFHVI+  + F++  G+ + + 
Sbjct: 177 LVFTGFQGMEYYQAPFTISDSIYGSTFF----LATGFHGFHVIIGTL-FLIICGIRQYLG 233

[Close]

BLAST of Carg25321 vs. TAIR 10
Match: ATMG00730.1 (cytochrome c oxidase subunit 3 )

HSP 1 Score: 112.1 bits (279), Expect = 8.1e-25
Identity = 83/185 (44.86%), Postives = 97/185 (52.43%), Query Frame = 0


Query: 1   MFVWWRDVLRESTLEGHHTKVVQLGLRYGFILFIVSEVMFLFAFFRASSHSSLAPTVEIG 60
           MFVWWRDVLRESTLEGHHTKVVQLG RYG ILFIVSEVMF FAFF ASSHSSLAP VEIG
Sbjct: 57  MFVWWRDVLRESTLEGHHTKVVQLGPRYGSILFIVSEVMFFFAFFWASSHSSLAPAVEIG 116

Query: 61  -------------------------AGAANIRDSSCYIRAGNSDKSSSVIRFIVATVSLA 120
                                    +  A +  +   I AG   K    +  +VATV LA
Sbjct: 117 GIWPPKGIEVLDPWEIPFLNTPILPSSGAAVTWAHHAILAG---KEKRAVYALVATVLLA 176

Query: 121 ---------ERINRLSKLVDSIIWVSLIAPSSYAPGFHGFHVIMTWIRFILSLGLYRLVE 152
                    E       + DSI   +       A GFHGFHVI+  + F++  G+ + + 
Sbjct: 177 LVFTGFQGMEYYQAPFTISDSIYGSTFF----LATGFHGFHVIIGTL-FLIICGIRQYLG 233

[Close]

BLAST of Carg25321 vs. TAIR 10
Match: AT2G46505.1 (succinate dehydrogenase subunit 4 )

HSP 1 Score: 57.0 bits (136), Expect = 3.1e-08
Identity = 28/52 (53.85%), Postives = 40/52 (76.92%), Query Frame = 0


Query: 193 FPTNLSVFWHINSGIEEIMADHVHQEMTRNWILVYLRLFLLIVIKDVFLSLI 245
           F   + V +HI+ G+EEI+AD+VHQEMTRN I++ L LF +IV+KD+ L L+
Sbjct: 100 FLRKIPVVFHIHEGMEEILADYVHQEMTRNLIVMSLGLFQIIVLKDIILFLL 151

The following BLAST results are available for this feature:

BLAST of Carg25321 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7022597.1	1.7e-165	100.00	Cytochrome c oxidase subunit 3, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]
KAE9584275.1	1.1e-55	49.84	putative cytochrome-c oxidase [Lupinus albus]	[more]
KAE8648258.1	1.1e-52	50.16	hypothetical protein Csa_018513 [Cucumis sativus]	[more]
TYJ97776.1	2.3e-50	48.84	cytochrome c oxidase subunit 3 [Cucumis melo var. makuwa]	[more]
GAY69439.1	1.2e-46	47.39	hypothetical protein CUMW_272010 [Citrus unshiu]	[more]

BLAST of Carg25321 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P32808	8.4e-27	47.57	Cytochrome c oxidase subunit 3 OS=Helianthus annuus OX=4232 GN=COX3 PE=3 SV=1	[more]
P08745	5.5e-26	47.51	Cytochrome c oxidase subunit 3 OS=Oenothera berteroana OX=3950 GN=COX3 PE=3 SV=2	[more]
Q03227	7.1e-26	46.70	Cytochrome c oxidase subunit 3 OS=Vicia faba OX=3906 GN=COX3 PE=3 SV=1	[more]
Q36952	3.5e-25	48.00	Cytochrome c oxidase subunit 3 OS=Aegilops columnaris OX=4493 GN=COX3 PE=3 SV=1	[more]
P14853	1.3e-24	45.95	Cytochrome c oxidase subunit 3 OS=Glycine max OX=3847 GN=COX3 PE=2 SV=1	[more]

BLAST of Carg25321 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6A4MVS0	5.3e-56	49.84	Cytochrome c oxidase subunit 3 OS=Lupinus albus OX=3870 GN=Lalb_Chr00c24g0406721...	[more]
A0A6N2KUH8	1.4e-53	49.19	Cytochrome c oxidase subunit 3 OS=Salix viminalis OX=40686 GN=SVIM_LOCUS97736 PE...	[more]
A0A6N2KDM9	4.6e-52	47.96	Cytochrome c oxidase subunit 3 OS=Salix viminalis OX=40686 GN=SVIM_LOCUS71748 PE...	[more]
A0A5D3BFB3	1.1e-50	48.84	Cytochrome c oxidase subunit 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A2H5QXW9	5.8e-47	47.39	Cytochrome c oxidase subunit 3 OS=Citrus unshiu OX=55188 GN=CUMW_272010 PE=3 SV=...	[more]

BLAST of Carg25321 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G07687.1	8.1e-25	44.86	Cytochrome c oxidase, subunit III	[more]
ATMG00730.1	8.1e-25	44.86	cytochrome c oxidase subunit 3	[more]
AT2G46505.1	3.1e-08	53.85	succinate dehydrogenase subunit 4	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000298	Cytochrome c oxidase subunit III-like	PFAM	PF00510	COX3	coord: 1..61 e-value: 3.1E-21 score: 76.4
IPR000298	Cytochrome c oxidase subunit III-like	PROSITE	PS50253	COX3	coord: 1..61 score: 13.945756
IPR013833	Cytochrome c oxidase, subunit III, 4-helical bundle	GENE3D	1.20.120.80		coord: 18..96 e-value: 2.5E-6 score: 29.2
None	No IPR available	PANTHER	PTHR36358:SF1	SUCCINATE DEHYDROGENASE SUBUNIT 4, MITOCHONDRIAL	coord: 141..246
IPR044963	Succinate dehydrogenase subunit 4, mitochondrial	PANTHER	PTHR36358	SUCCINATE DEHYDROGENASE SUBUNIT 4, MITOCHONDRIAL	coord: 141..246
IPR035973	Cytochrome c oxidase subunit III-like superfamily	SUPERFAMILY	81452	Cytochrome c oxidase subunit III-like	coord: 1..61

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25321-RA	Carg25321-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006121	mitochondrial electron transport, succinate to ubiquinone
biological_process	GO:0006099	tricarboxylic acid cycle
biological_process	GO:0022904	respiratory electron transport chain
cellular_component	GO:0005749	mitochondrial respiratory chain complex II, succinate dehydrogenase complex (ubiquinone)
cellular_component	GO:0016020	membrane
cellular_component	GO:0005743	mitochondrial inner membrane
molecular_function	GO:0004129	cytochrome-c oxidase activity
molecular_function	GO:0009055	electron transfer activity