Carg25303 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25303
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionUPF0496 protein At3g19330-like
LocationCarg_Chr11: 10458362 .. 10459276 (+)
RNA-Seq ExpressionCarg25303
SyntenyCarg25303
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGCCTCACCTAGATCTACCAATCTCTCTCGCATATACACCATCAATGTCCTCCATGCCGATTCCTACAAGGAGATGTCGAGAATCACCAGCTCCATGGACCTTGTGGTCGCCGATCAAGACCACCTGGTGCTCGCTCAGGTGCTTCGCCCTGATCGAGATCACGTCCACAAGGTTCTTCACCGATCTAAATCCAAATCCCTTTCTGAACTCATATCTTCTTACTTCGATAACAGCGAGAAAGCCACCAGATTCTGCCTCTCCCTCCGCCGCTGCATCGCCGAAGCCCGGAGATTGTATCGGCCAATCCAAAACCTTATCGACCAACTTCCACAGTCCCACTGTGACCTCGTCTTCCAAGAATTCTGTAAATTCGATAAGGAACACAATCCTTTCCCCGGCCCTGACTCACCGACCTTAGACCAAATCCGTGACCTCTTCGGCGTTCTCAATCGGCAACTGGAACGCCGCCTTAACAAATCCAGTTCCAGGATTCGCTTTGTTCGCCCTCCCACCGTCTGCTGCAGCGGCGATGCCATCACCGCCTATATTCCTTCTAGGTTCGCCAGGAGAGAATTGGCGTATGCAGCTCAGATCAAAGTTGCATCTAGGAATACCTATATCCTAAAGACTGACCTTGACACGCTTGATAGCCTCGTTAGGCGTTTGCATAACGCCGTGGAGGGCGACAAGCGTTTCATTCAGATGGGTTTGGGCATAATGAATGATAATAACACAGCTCAGGAAGTACTCAAACATTTGGAGATGAACCAACAGAATTTGAGGCATTACCTGGATCTTCTTGAGGAAAAGATAACAACTTGTTTGATCACTGTCAACCAGTCAAGATCTCTACTACTCCAGGAGATTTTACATCATCAATCTAAAGCTGAGTCTTCTCATTCCCAATAG

mRNA sequence

ATGGATGCCTCACCTAGATCTACCAATCTCTCTCGCATATACACCATCAATGTCCTCCATGCCGATTCCTACAAGGAGATGTCGAGAATCACCAGCTCCATGGACCTTGTGGTCGCCGATCAAGACCACCTGGTGCTCGCTCAGGTGCTTCGCCCTGATCGAGATCACGTCCACAAGGTTCTTCACCGATCTAAATCCAAATCCCTTTCTGAACTCATATCTTCTTACTTCGATAACAGCGAGAAAGCCACCAGATTCTGCCTCTCCCTCCGCCGCTGCATCGCCGAAGCCCGGAGATTGTATCGGCCAATCCAAAACCTTATCGACCAACTTCCACAGTCCCACTGTGACCTCGTCTTCCAAGAATTCTGTAAATTCGATAAGGAACACAATCCTTTCCCCGGCCCTGACTCACCGACCTTAGACCAAATCCGTGACCTCTTCGGCGTTCTCAATCGGCAACTGGAACGCCGCCTTAACAAATCCAGTTCCAGGATTCGCTTTGTTCGCCCTCCCACCGTCTGCTGCAGCGGCGATGCCATCACCGCCTATATTCCTTCTAGGTTCGCCAGGAGAGAATTGGCGTATGCAGCTCAGATCAAAGTTGCATCTAGGAATACCTATATCCTAAAGACTGACCTTGACACGCTTGATAGCCTCGTTAGGCGTTTGCATAACGCCGTGGAGGGCGACAAGCGTTTCATTCAGATGGGTTTGGGCATAATGAATGATAATAACACAGCTCAGGAAGTACTCAAACATTTGGAGATGAACCAACAGAATTTGAGGCATTACCTGGATCTTCTTGAGGAAAAGATAACAACTTGTTTGATCACTGTCAACCAGTCAAGATCTCTACTACTCCAGGAGATTTTACATCATCAATCTAAAGCTGAGTCTTCTCATTCCCAATAG

Coding sequence (CDS)

ATGGATGCCTCACCTAGATCTACCAATCTCTCTCGCATATACACCATCAATGTCCTCCATGCCGATTCCTACAAGGAGATGTCGAGAATCACCAGCTCCATGGACCTTGTGGTCGCCGATCAAGACCACCTGGTGCTCGCTCAGGTGCTTCGCCCTGATCGAGATCACGTCCACAAGGTTCTTCACCGATCTAAATCCAAATCCCTTTCTGAACTCATATCTTCTTACTTCGATAACAGCGAGAAAGCCACCAGATTCTGCCTCTCCCTCCGCCGCTGCATCGCCGAAGCCCGGAGATTGTATCGGCCAATCCAAAACCTTATCGACCAACTTCCACAGTCCCACTGTGACCTCGTCTTCCAAGAATTCTGTAAATTCGATAAGGAACACAATCCTTTCCCCGGCCCTGACTCACCGACCTTAGACCAAATCCGTGACCTCTTCGGCGTTCTCAATCGGCAACTGGAACGCCGCCTTAACAAATCCAGTTCCAGGATTCGCTTTGTTCGCCCTCCCACCGTCTGCTGCAGCGGCGATGCCATCACCGCCTATATTCCTTCTAGGTTCGCCAGGAGAGAATTGGCGTATGCAGCTCAGATCAAAGTTGCATCTAGGAATACCTATATCCTAAAGACTGACCTTGACACGCTTGATAGCCTCGTTAGGCGTTTGCATAACGCCGTGGAGGGCGACAAGCGTTTCATTCAGATGGGTTTGGGCATAATGAATGATAATAACACAGCTCAGGAAGTACTCAAACATTTGGAGATGAACCAACAGAATTTGAGGCATTACCTGGATCTTCTTGAGGAAAAGATAACAACTTGTTTGATCACTGTCAACCAGTCAAGATCTCTACTACTCCAGGAGATTTTACATCATCAATCTAAAGCTGAGTCTTCTCATTCCCAATAG

Protein sequence

MDASPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVADQDHLVLAQVLRPDRDHVHKVLHRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEARRLYRPIQNLIDQLPQSHCDLVFQEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPPTVCCSGDAITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLGIMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQSKAESSHSQ
Homology
BLAST of Carg25303 vs. NCBI nr
Match: KAG6588857.1 (UPF0496 protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022615.1 UPF0496 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 598.2 bits (1541), Expect = 4.0e-167
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 1   MDASPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVADQDHLVLAQVLRPDRDHVHKV 60
           MDASPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVADQDHLVLAQVLRPDRDHVHKV
Sbjct: 1   MDASPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVADQDHLVLAQVLRPDRDHVHKV 60

Query: 61  LHRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEARRLYRPIQNLIDQLPQSHCDLVF 120
           LHRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEARRLYRPIQNLIDQLPQSHCDLVF
Sbjct: 61  LHRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEARRLYRPIQNLIDQLPQSHCDLVF 120

Query: 121 QEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPPTVCCSGDA 180
           QEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPPTVCCSGDA
Sbjct: 121 QEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPPTVCCSGDA 180

Query: 181 ITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLG 240
           ITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLG
Sbjct: 181 ITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLG 240

Query: 241 IMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQSKAES 300
           IMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQSKAES
Sbjct: 241 IMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQSKAES 300

Query: 301 SHSQ 305
           SHSQ
Sbjct: 301 SHSQ 304

BLAST of Carg25303 vs. NCBI nr
Match: XP_022928413.1 (UPF0496 protein At3g19330-like [Cucurbita moschata])

HSP 1 Score: 596.3 bits (1536), Expect = 1.5e-166
Identity = 302/304 (99.34%), Postives = 303/304 (99.67%), Query Frame = 0

Query: 1   MDASPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVADQDHLVLAQVLRPDRDHVHKV 60
           MDASPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVADQDHLVLAQVLRPDRDHVHKV
Sbjct: 1   MDASPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVADQDHLVLAQVLRPDRDHVHKV 60

Query: 61  LHRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEARRLYRPIQNLIDQLPQSHCDLVF 120
           LHRSKSKSLSEL+SSYFDNSEK TRFCLSLRRCIAEARRLYRPIQNLIDQLPQSHCDLVF
Sbjct: 61  LHRSKSKSLSELVSSYFDNSEKTTRFCLSLRRCIAEARRLYRPIQNLIDQLPQSHCDLVF 120

Query: 121 QEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPPTVCCSGDA 180
           QEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPPTVCCSGDA
Sbjct: 121 QEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPPTVCCSGDA 180

Query: 181 ITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLG 240
           ITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLG
Sbjct: 181 ITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLG 240

Query: 241 IMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQSKAES 300
           IMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQSKAES
Sbjct: 241 IMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQSKAES 300

Query: 301 SHSQ 305
           SHSQ
Sbjct: 301 SHSQ 304

BLAST of Carg25303 vs. NCBI nr
Match: XP_023531167.1 (UPF0496 protein At3g19330-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 585.5 bits (1508), Expect = 2.7e-163
Identity = 299/304 (98.36%), Postives = 301/304 (99.01%), Query Frame = 0

Query: 1   MDASPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVADQDHLVLAQVLRPDRDHVHKV 60
           MDASPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVADQDHL+LAQVLRPDRDHVHKV
Sbjct: 1   MDASPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVADQDHLMLAQVLRPDRDHVHKV 60

Query: 61  LHRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEARRLYRPIQNLIDQLPQSHCDLVF 120
           LHRSKSKSLSELISSYFDNSEK TRFCLSLRRCIAEARRLYRPIQNLIDQLPQSHCDLVF
Sbjct: 61  LHRSKSKSLSELISSYFDNSEKTTRFCLSLRRCIAEARRLYRPIQNLIDQLPQSHCDLVF 120

Query: 121 QEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPPTVCCSGDA 180
           QEF KFDKEHNPFPG DSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPPTVCCSGDA
Sbjct: 121 QEFGKFDKEHNPFPGLDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPPTVCCSGDA 180

Query: 181 ITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLG 240
           ITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLG
Sbjct: 181 ITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLG 240

Query: 241 IMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQSKAES 300
           I+NDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQSKAES
Sbjct: 241 IVNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQSKAES 300

Query: 301 SHSQ 305
           SHSQ
Sbjct: 301 SHSQ 304

BLAST of Carg25303 vs. NCBI nr
Match: XP_022989697.1 (UPF0496 protein At3g19250-like [Cucurbita maxima])

HSP 1 Score: 554.7 bits (1428), Expect = 5.0e-154
Identity = 286/306 (93.46%), Postives = 294/306 (96.08%), Query Frame = 0

Query: 1   MDASPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVADQDHLVLAQVLRPDRDHVHKV 60
           MD+SPRSTNLS IYTINVLHADSYKEMSRITSSMDLVVADQDHL+LAQVLRPDRDHVHKV
Sbjct: 1   MDSSPRSTNLSPIYTINVLHADSYKEMSRITSSMDLVVADQDHLMLAQVLRPDRDHVHKV 60

Query: 61  LHR--SKSKSLSELISSYFDNSEKATRFCLSLRRCIAEARRLYRPIQNLIDQLPQSHCDL 120
           LHR  SKSKSLSELISSYFDNSEK TRFCLSLRRCIAEA RLYRPIQNLIDQLPQSHCDL
Sbjct: 61  LHRSKSKSKSLSELISSYFDNSEKTTRFCLSLRRCIAEAWRLYRPIQNLIDQLPQSHCDL 120

Query: 121 VFQEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPPTVCCSG 180
           VFQEF KFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRL+KSSSRIRFVRPPTVCCSG
Sbjct: 121 VFQEFGKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLSKSSSRIRFVRPPTVCCSG 180

Query: 181 DAITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMG 240
            AITAY  ++F RRELAY AQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKR+IQMG
Sbjct: 181 AAITAYTLAKFTRRELAYEAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRYIQMG 240

Query: 241 LGIMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQSKA 300
           LGIMNDNNTAQEVLKHLEM+QQNLRH LDLLE+KITTCLITVNQSRSLLLQEILHHQSKA
Sbjct: 241 LGIMNDNNTAQEVLKHLEMDQQNLRHCLDLLEQKITTCLITVNQSRSLLLQEILHHQSKA 300

Query: 301 ESSHSQ 305
           ESSHSQ
Sbjct: 301 ESSHSQ 306

BLAST of Carg25303 vs. NCBI nr
Match: XP_008449986.1 (PREDICTED: UPF0496 protein At3g19330 isoform X2 [Cucumis melo])

HSP 1 Score: 360.5 bits (924), Expect = 1.4e-95
Identity = 207/323 (64.09%), Postives = 234/323 (72.45%), Query Frame = 0

Query: 4   SPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVAD-----QDHL-----VLAQVLRPD 63
           S  + NLSRIY+  +L  +S+KEM RITSSMDLVV D     QD L     +L QVL P+
Sbjct: 13  STTTINLSRIYSTAILQTNSFKEMVRITSSMDLVVPDAADHEQDQLQDLSGMLTQVLSPN 72

Query: 64  RDHVHKVLHRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEARRLYRPIQNL-----I 123
           RD V   LHRSKS SL  LISSYFD SE+ TR CL+LR  +A+ARRLY PI  L     I
Sbjct: 73  RDRVLNALHRSKSTSLLNLISSYFDYSERTTRLCLTLRGIVAQARRLYSPINTLLQDLPI 132

Query: 124 DQLPQSHCDLVFQEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRF 183
           D L Q+HC+LV QEF KF  + NPFP PDS T   IRD F  LNRQLERRL KSSSRIR 
Sbjct: 133 DSLSQTHCELVVQEFHKFKDDANPFPRPDSLTFHPIRDHFCDLNRQLERRLRKSSSRIRL 192

Query: 184 VRPP------TVCC-SGDAITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLV 243
           VR P       +CC  G AITAYIP +FARRELAYAAQ+KVASRNTY+LKTDLDTLDSLV
Sbjct: 193 VRSPNPDPNTVLCCRGGAAITAYIPPKFARRELAYAAQLKVASRNTYVLKTDLDTLDSLV 252

Query: 244 RRLHNAVEGDKRFIQMGLGIMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVN 303
            RLHN VE DKRFI++GL + ND +T QEVL HL  N  NL HYLDLLE+KITTCLITVN
Sbjct: 253 GRLHNTVEDDKRFIRLGLNMGNDEHTVQEVLIHLGKNHPNLGHYLDLLEQKITTCLITVN 312

Query: 304 QSRSLLLQEILHHQSKAESSHSQ 305
            SRS LL+EIL HQ+ ++SSH Q
Sbjct: 313 HSRSQLLKEILLHQTSSDSSHPQ 335

BLAST of Carg25303 vs. ExPASy Swiss-Prot
Match: Q9LT84 (UPF0496 protein At3g19330 OS=Arabidopsis thaliana OX=3702 GN=At3g19330 PE=2 SV=2)

HSP 1 Score: 162.2 bits (409), Expect = 9.5e-39
Identity = 114/328 (34.76%), Postives = 166/328 (50.61%), Query Frame = 0

Query: 9   NLSRIYTINVLHADSYKEM-SRITSSMDL------VVADQDHLVLAQVLRPDRDHVHKVL 68
           NLSR    +     SY ++ SR+   +DL      ++     L+L+QVL+P+++ V + +
Sbjct: 46  NLSR-ELAHAFQTPSYHDVRSRVHVVVDLTQIHHRLIQPDIELLLSQVLQPNKECVQEAI 105

Query: 69  HRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEAR-RLYRPIQNLI-----DQLP--- 128
              K  +L+ L+S+YF +SE ATR CL+L + +  AR  LY P+ +L      D LP   
Sbjct: 106 RHVKQTTLTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDLFNIFPGDSLPAID 165

Query: 129 QSHCDLVFQEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVR-- 188
           +S CDL F  F K D   NPF  P+S +    +  F  L   L+RRL KS SR+R +   
Sbjct: 166 ESLCDLAFDVFLKLDTFENPFSSPESYSFRDTQLCFSQLKHNLDRRLRKSRSRVRLIHHA 225

Query: 189 ---------------------------PPTVCCSGDAITAYIPSRFARRELAYAAQIKVA 248
                                      P  +  +G   + Y+P  F R+EL    Q+  A
Sbjct: 226 TAGSSLCLVAAVVAVAASAVVIASHALPILLVVAGPLCSPYLPHSFKRKELTNICQLNAA 285

Query: 249 SRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLGIMNDNNTAQEVLKHLEMNQQNLR 292
           S+ T++L  DLDT+D LV RLH  +E DK  I++GL    D ++ QE+LK L  +   L 
Sbjct: 286 SKGTFVLNKDLDTIDRLVSRLHTGIEYDKFLIRLGLERGRDVHSIQEILKLLRKSHLPLT 345

BLAST of Carg25303 vs. ExPASy Swiss-Prot
Match: Q9LJK4 (UPF0496 protein At3g19250 OS=Arabidopsis thaliana OX=3702 GN=At3g19250 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 8.9e-37
Identity = 102/293 (34.81%), Postives = 150/293 (51.19%), Query Frame = 0

Query: 44  LVLAQVLRPDRDHVHKV--LHRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEAR-RL 103
           L L+Q LRP+ + V +   L  +K  +L+ L+S+YF +SE ATRFCL+L + +  AR  L
Sbjct: 68  LFLSQELRPNNESVQEALSLRHAKQTTLTNLVSTYFQHSEDATRFCLNLYQNVHSARCHL 127

Query: 104 YRPIQNLIDQLP--------QSHCDLVFQEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLN 163
           Y P+ +L +  P        +S C+L F  F K D   NPF  P+S +    +  F  L 
Sbjct: 128 YTPLLDLFNIFPRDSHSAIDESFCNLAFDVFLKLDTFENPFASPESHSFQDTQLCFYQLA 187

Query: 164 RQLERRLNKSSSRIRFVR-----------------------------PPTVCCSGDAITA 223
            +L+ R+ KS SR+R +                              P  +  +G   T 
Sbjct: 188 DKLDTRIRKSKSRVRLLHHATAGSALCLVTAVVVVAASAAFIAYHALPTILVVAGPLCTP 247

Query: 224 YIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLGIMN 283
           Y+P  F ++EL+   Q+ VA++ T+ L  DLDT+D LV RLH  V+ DK  I++GL    
Sbjct: 248 YLPHSFKKKELSNIFQLNVAAKGTFALNKDLDTIDRLVSRLHTGVKNDKLLIRLGLERGR 307

Query: 284 DNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQS 297
           D  T  E +K L  +  N  H L++L + I      VN+SRSLLL+EIL  Q+
Sbjct: 308 DVYTIPEFVKQLRKSHVNHTHQLEVLVDHICRWFTNVNKSRSLLLKEILRPQT 360

BLAST of Carg25303 vs. ExPASy Swiss-Prot
Match: Q9SMU4 (UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 2.6e-12
Identity = 68/273 (24.91%), Postives = 127/273 (46.52%), Query Frame = 0

Query: 49  VLRPDRDHVHKVLHRSK-SKSLSELISSYFDNSEKATRFCLSLRRCIAEARRLYRPIQNL 108
           +L PD + + ++L  S+  +    L+S YF  +  A   C  L + I   R  Y  ++  
Sbjct: 129 LLDPDLNTITRILDVSRVGRHTRTLLSDYFLETANAFLLCTQLLKNIHHLRSKYESLKPK 188

Query: 109 IDQLPQSHCDL-VFQEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRI 168
                ++H  L +  +F +  K  +PF    S  +  IR     L ++LE R +K+ +++
Sbjct: 189 FHS--ENHNSLALIDQFTEISKWFDPFISSGS-RIQLIRSGCLYLLKRLESRRDKTRAKL 248

Query: 169 RFVRPPT---------------VCCSGDAITAYI-----------PSRFARRELAYAAQI 228
           + +   T               V  +  A   ++           P+    +    AA++
Sbjct: 249 KLINGLTHSSGLLVLALTTTLIVTIASHAFALFLAAPTLLASQFKPAGLRNKLTKTAARL 308

Query: 229 KVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLGIMNDN-NTAQEVLKHLEMNQ 288
            VA++ TYIL  DLDT+  LV R+++ V   +   +  +G  +     ++EV + L+  +
Sbjct: 309 DVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFWVGRGSGRVRGSEEVARELKRCE 368

Query: 289 QNLRHYLDLLEEKITTCLITVNQSRSLLLQEIL 293
           ++    LD LEE I  C +T+N++R+LL++EIL
Sbjct: 369 ESFSEELDELEEHIYLCFMTINRARNLLVKEIL 398

BLAST of Carg25303 vs. ExPASy Swiss-Prot
Match: A2XCJ1 (UPF0496 protein 3 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_009784 PE=3 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 5.1e-08
Identity = 71/291 (24.40%), Postives = 117/291 (40.21%), Query Frame = 0

Query: 44  LVLAQVLRPDRDHVHKVLHRSKSKSLSE----LISSYFDNSEKATRFCLSLRRCIAEARR 103
           L    +L PD+  V   L   +   L      L+++Y+  +  A+  C  L + I   R 
Sbjct: 86  LFAEHLLEPDQRAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRL 145

Query: 104 LYRPIQNLIDQLPQSHCDLVFQEFCKFDKE-HNPFP--GPDSPTLDQIRDLFGVLNRQLE 163
            YRP+++ + +L     D+              PF         L +++   G L R L+
Sbjct: 146 RYRPLKHTLRKLAS---DVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLLRGLD 205

Query: 164 RRLNKSSSRIR------------FVRPPTV-----CCSGDAITA---------------- 223
               K+  RIR            FV    V      C G  I A                
Sbjct: 206 AGRKKARHRIRSVARLRRALSVSFVTAVAVVAVVGACIGVHILAAFAAFPMMSPAWLGER 265

Query: 224 YIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQM------ 283
           +   R ARR L    Q++ A++ TYIL  D++T+  LV R+ +  E     +++      
Sbjct: 266 FFSGRAARRAL---VQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALLRLCVEHRP 325

Query: 284 GLGIMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLL 289
             G        QEVL+ L  N+++ R  LD LEE +  C +T+N++R +++
Sbjct: 326 AAGAGGKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTINKARIMVM 370

BLAST of Carg25303 vs. ExPASy Swiss-Prot
Match: Q10RR9 (UPF0496 protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0148000 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 4.3e-07
Identity = 73/293 (24.91%), Postives = 116/293 (39.59%), Query Frame = 0

Query: 44  LVLAQVLRPDRDHVHKVLHRSKSKSLSE----LISSYFDNSEKATRFCLSLRRCIAEARR 103
           L    +L PD+  V   L   +   L      L+++Y+  +  A+  C  L + I   R 
Sbjct: 86  LFAEHLLEPDQRAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRL 145

Query: 104 LYRPIQNLIDQLPQSHCDLVFQEFCKFDKE-HNPFP--GPDSPTLDQIRDLFGVLNRQLE 163
            YRP+++ + +L     D+              PF         L +++   G L R L+
Sbjct: 146 RYRPLKHTLRKLAS---DVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLLRGLD 205

Query: 164 RRLNKSSSRIR------------FVRPPTV-----CCSGDAITA---------------- 223
               K+  RIR            FV    V      C G  I A                
Sbjct: 206 AGRKKARHRIRSVARLRRALSVSFVTAVAVVAVVGACIGVHILAAFAAFPMMSPAWLGER 265

Query: 224 YIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGL---- 283
           +   R ARR L    Q++ A++ TYIL  D++T+  LV R+ +  EG+       L    
Sbjct: 266 FFSGRAARRAL---VQLEAAAKGTYILNRDMETISRLVARVRD--EGEHMVALRRLCVEH 325

Query: 284 ----GIMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLL 289
               G        QEVL+ L  N+++ R  LD LEE +  C +T N++R +++
Sbjct: 326 RPAAGAGGKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTTNKARIMVM 370

BLAST of Carg25303 vs. ExPASy TrEMBL
Match: A0A6J1ENY5 (UPF0496 protein At3g19330-like OS=Cucurbita moschata OX=3662 GN=LOC111435234 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 7.3e-167
Identity = 302/304 (99.34%), Postives = 303/304 (99.67%), Query Frame = 0

Query: 1   MDASPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVADQDHLVLAQVLRPDRDHVHKV 60
           MDASPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVADQDHLVLAQVLRPDRDHVHKV
Sbjct: 1   MDASPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVADQDHLVLAQVLRPDRDHVHKV 60

Query: 61  LHRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEARRLYRPIQNLIDQLPQSHCDLVF 120
           LHRSKSKSLSEL+SSYFDNSEK TRFCLSLRRCIAEARRLYRPIQNLIDQLPQSHCDLVF
Sbjct: 61  LHRSKSKSLSELVSSYFDNSEKTTRFCLSLRRCIAEARRLYRPIQNLIDQLPQSHCDLVF 120

Query: 121 QEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPPTVCCSGDA 180
           QEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPPTVCCSGDA
Sbjct: 121 QEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPPTVCCSGDA 180

Query: 181 ITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLG 240
           ITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLG
Sbjct: 181 ITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLG 240

Query: 241 IMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQSKAES 300
           IMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQSKAES
Sbjct: 241 IMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQSKAES 300

Query: 301 SHSQ 305
           SHSQ
Sbjct: 301 SHSQ 304

BLAST of Carg25303 vs. ExPASy TrEMBL
Match: A0A6J1JGK1 (UPF0496 protein At3g19250-like OS=Cucurbita maxima OX=3661 GN=LOC111486700 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.4e-154
Identity = 286/306 (93.46%), Postives = 294/306 (96.08%), Query Frame = 0

Query: 1   MDASPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVADQDHLVLAQVLRPDRDHVHKV 60
           MD+SPRSTNLS IYTINVLHADSYKEMSRITSSMDLVVADQDHL+LAQVLRPDRDHVHKV
Sbjct: 1   MDSSPRSTNLSPIYTINVLHADSYKEMSRITSSMDLVVADQDHLMLAQVLRPDRDHVHKV 60

Query: 61  LHR--SKSKSLSELISSYFDNSEKATRFCLSLRRCIAEARRLYRPIQNLIDQLPQSHCDL 120
           LHR  SKSKSLSELISSYFDNSEK TRFCLSLRRCIAEA RLYRPIQNLIDQLPQSHCDL
Sbjct: 61  LHRSKSKSKSLSELISSYFDNSEKTTRFCLSLRRCIAEAWRLYRPIQNLIDQLPQSHCDL 120

Query: 121 VFQEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPPTVCCSG 180
           VFQEF KFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRL+KSSSRIRFVRPPTVCCSG
Sbjct: 121 VFQEFGKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLSKSSSRIRFVRPPTVCCSG 180

Query: 181 DAITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMG 240
            AITAY  ++F RRELAY AQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKR+IQMG
Sbjct: 181 AAITAYTLAKFTRRELAYEAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRYIQMG 240

Query: 241 LGIMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQSKA 300
           LGIMNDNNTAQEVLKHLEM+QQNLRH LDLLE+KITTCLITVNQSRSLLLQEILHHQSKA
Sbjct: 241 LGIMNDNNTAQEVLKHLEMDQQNLRHCLDLLEQKITTCLITVNQSRSLLLQEILHHQSKA 300

Query: 301 ESSHSQ 305
           ESSHSQ
Sbjct: 301 ESSHSQ 306

BLAST of Carg25303 vs. ExPASy TrEMBL
Match: A0A5D3DUJ9 (UPF0496 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold236G00350 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 6.7e-96
Identity = 206/323 (63.78%), Postives = 234/323 (72.45%), Query Frame = 0

Query: 4   SPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVAD-----QDHL-----VLAQVLRPD 63
           S  + NLSRIY+  +L  +S+KEM RITSSMDLVV D     QD L     +L QVL P+
Sbjct: 56  STTTINLSRIYSTAILQTNSFKEMVRITSSMDLVVPDAADHEQDQLQDLSGMLTQVLSPN 115

Query: 64  RDHVHKVLHRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEARRLYRPIQNLIDQLP- 123
           RD V   LHRSKS SL  LISSYFD SE+ TR CL+LR  +A+ARRLY PI  L+  LP 
Sbjct: 116 RDRVLNALHRSKSTSLLNLISSYFDYSERTTRLCLTLRGIVAQARRLYSPINTLLQDLPI 175

Query: 124 ----QSHCDLVFQEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRF 183
               Q+HC+LV QEF KF  + NPFP PDS T   IRD F  LNRQLERRL KSSSRIR 
Sbjct: 176 GSLSQTHCELVVQEFHKFKDDANPFPRPDSLTFHPIRDHFCDLNRQLERRLRKSSSRIRL 235

Query: 184 VRPP------TVCC-SGDAITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLV 243
           VR P       +CC  G AITAYIP +FARRELAYAAQ+KVASRNTY+LKTDLDTLDSLV
Sbjct: 236 VRSPNPDPNTVLCCRGGAAITAYIPPKFARRELAYAAQLKVASRNTYVLKTDLDTLDSLV 295

Query: 244 RRLHNAVEGDKRFIQMGLGIMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVN 303
            RLHN VE DKRFI++GL + ND +T QEVL HL  N  NL HYLDLLE+KITTCLITVN
Sbjct: 296 GRLHNTVEDDKRFIRLGLNMGNDEHTVQEVLIHLGKNHPNLGHYLDLLEQKITTCLITVN 355

Query: 304 QSRSLLLQEILHHQSKAESSHSQ 305
            SRS LL+EIL HQ+ ++SSH Q
Sbjct: 356 HSRSQLLKEILLHQTSSDSSHPQ 378

BLAST of Carg25303 vs. ExPASy TrEMBL
Match: A0A1S3BP81 (UPF0496 protein At3g19330 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491707 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 6.7e-96
Identity = 207/323 (64.09%), Postives = 234/323 (72.45%), Query Frame = 0

Query: 4   SPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVAD-----QDHL-----VLAQVLRPD 63
           S  + NLSRIY+  +L  +S+KEM RITSSMDLVV D     QD L     +L QVL P+
Sbjct: 32  STTTINLSRIYSTAILQTNSFKEMVRITSSMDLVVPDAADHEQDQLQDLSGMLTQVLSPN 91

Query: 64  RDHVHKVLHRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEARRLYRPIQNL-----I 123
           RD V   LHRSKS SL  LISSYFD SE+ TR CL+LR  +A+ARRLY PI  L     I
Sbjct: 92  RDRVLNALHRSKSTSLLNLISSYFDYSERTTRLCLTLRGIVAQARRLYSPINTLLQDLPI 151

Query: 124 DQLPQSHCDLVFQEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRF 183
           D L Q+HC+LV QEF KF  + NPFP PDS T   IRD F  LNRQLERRL KSSSRIR 
Sbjct: 152 DSLSQTHCELVVQEFHKFKDDANPFPRPDSLTFHPIRDHFCDLNRQLERRLRKSSSRIRL 211

Query: 184 VRPP------TVCC-SGDAITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLV 243
           VR P       +CC  G AITAYIP +FARRELAYAAQ+KVASRNTY+LKTDLDTLDSLV
Sbjct: 212 VRSPNPDPNTVLCCRGGAAITAYIPPKFARRELAYAAQLKVASRNTYVLKTDLDTLDSLV 271

Query: 244 RRLHNAVEGDKRFIQMGLGIMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVN 303
            RLHN VE DKRFI++GL + ND +T QEVL HL  N  NL HYLDLLE+KITTCLITVN
Sbjct: 272 GRLHNTVEDDKRFIRLGLNMGNDEHTVQEVLIHLGKNHPNLGHYLDLLEQKITTCLITVN 331

Query: 304 QSRSLLLQEILHHQSKAESSHSQ 305
            SRS LL+EIL HQ+ ++SSH Q
Sbjct: 332 HSRSQLLKEILLHQTSSDSSHPQ 354

BLAST of Carg25303 vs. ExPASy TrEMBL
Match: A0A1S3BMN5 (UPF0496 protein At3g19330 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491707 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 6.7e-96
Identity = 207/323 (64.09%), Postives = 234/323 (72.45%), Query Frame = 0

Query: 4   SPRSTNLSRIYTINVLHADSYKEMSRITSSMDLVVAD-----QDHL-----VLAQVLRPD 63
           S  + NLSRIY+  +L  +S+KEM RITSSMDLVV D     QD L     +L QVL P+
Sbjct: 13  STTTINLSRIYSTAILQTNSFKEMVRITSSMDLVVPDAADHEQDQLQDLSGMLTQVLSPN 72

Query: 64  RDHVHKVLHRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEARRLYRPIQNL-----I 123
           RD V   LHRSKS SL  LISSYFD SE+ TR CL+LR  +A+ARRLY PI  L     I
Sbjct: 73  RDRVLNALHRSKSTSLLNLISSYFDYSERTTRLCLTLRGIVAQARRLYSPINTLLQDLPI 132

Query: 124 DQLPQSHCDLVFQEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRF 183
           D L Q+HC+LV QEF KF  + NPFP PDS T   IRD F  LNRQLERRL KSSSRIR 
Sbjct: 133 DSLSQTHCELVVQEFHKFKDDANPFPRPDSLTFHPIRDHFCDLNRQLERRLRKSSSRIRL 192

Query: 184 VRPP------TVCC-SGDAITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLV 243
           VR P       +CC  G AITAYIP +FARRELAYAAQ+KVASRNTY+LKTDLDTLDSLV
Sbjct: 193 VRSPNPDPNTVLCCRGGAAITAYIPPKFARRELAYAAQLKVASRNTYVLKTDLDTLDSLV 252

Query: 244 RRLHNAVEGDKRFIQMGLGIMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVN 303
            RLHN VE DKRFI++GL + ND +T QEVL HL  N  NL HYLDLLE+KITTCLITVN
Sbjct: 253 GRLHNTVEDDKRFIRLGLNMGNDEHTVQEVLIHLGKNHPNLGHYLDLLEQKITTCLITVN 312

Query: 304 QSRSLLLQEILHHQSKAESSHSQ 305
            SRS LL+EIL HQ+ ++SSH Q
Sbjct: 313 HSRSQLLKEILLHQTSSDSSHPQ 335

BLAST of Carg25303 vs. TAIR 10
Match: AT3G19330.3 (Protein of unknown function (DUF677) )

HSP 1 Score: 172.2 bits (435), Expect = 6.5e-43
Identity = 114/299 (38.13%), Postives = 165/299 (55.18%), Query Frame = 0

Query: 9   NLSRIYTINVLHADSYKEM-SRITSSMDL------VVADQDHLVLAQVLRPDRDHVHKVL 68
           NLSR    +     SY ++ SR+   +DL      ++     L+L+QVL+P+++ V + +
Sbjct: 46  NLSR-ELAHAFQTPSYHDVRSRVHVVVDLTQIHHRLIQPDIELLLSQVLQPNKECVQEAI 105

Query: 69  HRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEAR-RLYRPIQNLI-----DQLP--- 128
              K  +L+ L+S+YF +SE ATR CL+L + +  AR  LY P+ +L      D LP   
Sbjct: 106 RHVKQTTLTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDLFNIFPGDSLPAID 165

Query: 129 QSHCDLVFQEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPP 188
           +S CDL F  F K D   NPF  P+S +    +  F  L   L+RRL KS SR+R +   
Sbjct: 166 ESLCDLAFDVFLKLDTFENPFSSPESYSFRDTQLCFSQLKHNLDRRLRKSRSRVRLIHHA 225

Query: 189 TVCCSGDAITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDK 248
           T   +G   + Y+P  F R+EL    Q+  AS+ T++L  DLDT+D LV RLH  +E DK
Sbjct: 226 T---AGPLCSPYLPHSFKRKELTNICQLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDK 285

Query: 249 RFIQMGLGIMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEI 292
             I++GL    D ++ QE+LK L  +   L H L  LE+ I      VN++RSLLL EI
Sbjct: 286 FLIRLGLERGRDVHSIQEILKLLRKSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTEI 340

BLAST of Carg25303 vs. TAIR 10
Match: AT3G19330.2 (Protein of unknown function (DUF677) )

HSP 1 Score: 172.2 bits (435), Expect = 6.5e-43
Identity = 114/299 (38.13%), Postives = 165/299 (55.18%), Query Frame = 0

Query: 9   NLSRIYTINVLHADSYKEM-SRITSSMDL------VVADQDHLVLAQVLRPDRDHVHKVL 68
           NLSR    +     SY ++ SR+   +DL      ++     L+L+QVL+P+++ V + +
Sbjct: 46  NLSR-ELAHAFQTPSYHDVRSRVHVVVDLTQIHHRLIQPDIELLLSQVLQPNKECVQEAI 105

Query: 69  HRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEAR-RLYRPIQNLI-----DQLP--- 128
              K  +L+ L+S+YF +SE ATR CL+L + +  AR  LY P+ +L      D LP   
Sbjct: 106 RHVKQTTLTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDLFNIFPGDSLPAID 165

Query: 129 QSHCDLVFQEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVRPP 188
           +S CDL F  F K D   NPF  P+S +    +  F  L   L+RRL KS SR+R +   
Sbjct: 166 ESLCDLAFDVFLKLDTFENPFSSPESYSFRDTQLCFSQLKHNLDRRLRKSRSRVRLIHHA 225

Query: 189 TVCCSGDAITAYIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDK 248
           T   +G   + Y+P  F R+EL    Q+  AS+ T++L  DLDT+D LV RLH  +E DK
Sbjct: 226 T---AGPLCSPYLPHSFKRKELTNICQLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDK 285

Query: 249 RFIQMGLGIMNDNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEI 292
             I++GL    D ++ QE+LK L  +   L H L  LE+ I      VN++RSLLL EI
Sbjct: 286 FLIRLGLERGRDVHSIQEILKLLRKSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTEI 340

BLAST of Carg25303 vs. TAIR 10
Match: AT3G19330.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 162.2 bits (409), Expect = 6.8e-40
Identity = 114/328 (34.76%), Postives = 166/328 (50.61%), Query Frame = 0

Query: 9   NLSRIYTINVLHADSYKEM-SRITSSMDL------VVADQDHLVLAQVLRPDRDHVHKVL 68
           NLSR    +     SY ++ SR+   +DL      ++     L+L+QVL+P+++ V + +
Sbjct: 46  NLSR-ELAHAFQTPSYHDVRSRVHVVVDLTQIHHRLIQPDIELLLSQVLQPNKECVQEAI 105

Query: 69  HRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEAR-RLYRPIQNLI-----DQLP--- 128
              K  +L+ L+S+YF +SE ATR CL+L + +  AR  LY P+ +L      D LP   
Sbjct: 106 RHVKQTTLTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDLFNIFPGDSLPAID 165

Query: 129 QSHCDLVFQEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRIRFVR-- 188
           +S CDL F  F K D   NPF  P+S +    +  F  L   L+RRL KS SR+R +   
Sbjct: 166 ESLCDLAFDVFLKLDTFENPFSSPESYSFRDTQLCFSQLKHNLDRRLRKSRSRVRLIHHA 225

Query: 189 ---------------------------PPTVCCSGDAITAYIPSRFARRELAYAAQIKVA 248
                                      P  +  +G   + Y+P  F R+EL    Q+  A
Sbjct: 226 TAGSSLCLVAAVVAVAASAVVIASHALPILLVVAGPLCSPYLPHSFKRKELTNICQLNAA 285

Query: 249 SRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLGIMNDNNTAQEVLKHLEMNQQNLR 292
           S+ T++L  DLDT+D LV RLH  +E DK  I++GL    D ++ QE+LK L  +   L 
Sbjct: 286 SKGTFVLNKDLDTIDRLVSRLHTGIEYDKFLIRLGLERGRDVHSIQEILKLLRKSHLPLT 345

BLAST of Carg25303 vs. TAIR 10
Match: AT3G19250.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 155.6 bits (392), Expect = 6.3e-38
Identity = 102/293 (34.81%), Postives = 150/293 (51.19%), Query Frame = 0

Query: 44  LVLAQVLRPDRDHVHKV--LHRSKSKSLSELISSYFDNSEKATRFCLSLRRCIAEAR-RL 103
           L L+Q LRP+ + V +   L  +K  +L+ L+S+YF +SE ATRFCL+L + +  AR  L
Sbjct: 68  LFLSQELRPNNESVQEALSLRHAKQTTLTNLVSTYFQHSEDATRFCLNLYQNVHSARCHL 127

Query: 104 YRPIQNLIDQLP--------QSHCDLVFQEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLN 163
           Y P+ +L +  P        +S C+L F  F K D   NPF  P+S +    +  F  L 
Sbjct: 128 YTPLLDLFNIFPRDSHSAIDESFCNLAFDVFLKLDTFENPFASPESHSFQDTQLCFYQLA 187

Query: 164 RQLERRLNKSSSRIRFVR-----------------------------PPTVCCSGDAITA 223
            +L+ R+ KS SR+R +                              P  +  +G   T 
Sbjct: 188 DKLDTRIRKSKSRVRLLHHATAGSALCLVTAVVVVAASAAFIAYHALPTILVVAGPLCTP 247

Query: 224 YIPSRFARRELAYAAQIKVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLGIMN 283
           Y+P  F ++EL+   Q+ VA++ T+ L  DLDT+D LV RLH  V+ DK  I++GL    
Sbjct: 248 YLPHSFKKKELSNIFQLNVAAKGTFALNKDLDTIDRLVSRLHTGVKNDKLLIRLGLERGR 307

Query: 284 DNNTAQEVLKHLEMNQQNLRHYLDLLEEKITTCLITVNQSRSLLLQEILHHQS 297
           D  T  E +K L  +  N  H L++L + I      VN+SRSLLL+EIL  Q+
Sbjct: 308 DVYTIPEFVKQLRKSHVNHTHQLEVLVDHICRWFTNVNKSRSLLLKEILRPQT 360

BLAST of Carg25303 vs. TAIR 10
Match: AT3G49070.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 74.3 bits (181), Expect = 1.9e-13
Identity = 68/273 (24.91%), Postives = 127/273 (46.52%), Query Frame = 0

Query: 49  VLRPDRDHVHKVLHRSK-SKSLSELISSYFDNSEKATRFCLSLRRCIAEARRLYRPIQNL 108
           +L PD + + ++L  S+  +    L+S YF  +  A   C  L + I   R  Y  ++  
Sbjct: 129 LLDPDLNTITRILDVSRVGRHTRTLLSDYFLETANAFLLCTQLLKNIHHLRSKYESLKPK 188

Query: 109 IDQLPQSHCDL-VFQEFCKFDKEHNPFPGPDSPTLDQIRDLFGVLNRQLERRLNKSSSRI 168
                ++H  L +  +F +  K  +PF    S  +  IR     L ++LE R +K+ +++
Sbjct: 189 FHS--ENHNSLALIDQFTEISKWFDPFISSGS-RIQLIRSGCLYLLKRLESRRDKTRAKL 248

Query: 169 RFVRPPT---------------VCCSGDAITAYI-----------PSRFARRELAYAAQI 228
           + +   T               V  +  A   ++           P+    +    AA++
Sbjct: 249 KLINGLTHSSGLLVLALTTTLIVTIASHAFALFLAAPTLLASQFKPAGLRNKLTKTAARL 308

Query: 229 KVASRNTYILKTDLDTLDSLVRRLHNAVEGDKRFIQMGLGIMNDN-NTAQEVLKHLEMNQ 288
            VA++ TYIL  DLDT+  LV R+++ V   +   +  +G  +     ++EV + L+  +
Sbjct: 309 DVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFWVGRGSGRVRGSEEVARELKRCE 368

Query: 289 QNLRHYLDLLEEKITTCLITVNQSRSLLLQEIL 293
           ++    LD LEE I  C +T+N++R+LL++EIL
Sbjct: 369 ESFSEELDELEEHIYLCFMTINRARNLLVKEIL 398

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6588857.14.0e-167100.00UPF0496 protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022615.1 U... [more]
XP_022928413.11.5e-16699.34UPF0496 protein At3g19330-like [Cucurbita moschata][more]
XP_023531167.12.7e-16398.36UPF0496 protein At3g19330-like [Cucurbita pepo subsp. pepo][more]
XP_022989697.15.0e-15493.46UPF0496 protein At3g19250-like [Cucurbita maxima][more]
XP_008449986.11.4e-9564.09PREDICTED: UPF0496 protein At3g19330 isoform X2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9LT849.5e-3934.76UPF0496 protein At3g19330 OS=Arabidopsis thaliana OX=3702 GN=At3g19330 PE=2 SV=2[more]
Q9LJK48.9e-3734.81UPF0496 protein At3g19250 OS=Arabidopsis thaliana OX=3702 GN=At3g19250 PE=2 SV=1[more]
Q9SMU42.6e-1224.91UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1[more]
A2XCJ15.1e-0824.40UPF0496 protein 3 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_009784 PE=3 SV=2[more]
Q10RR94.3e-0724.91UPF0496 protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0148000 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1ENY57.3e-16799.34UPF0496 protein At3g19330-like OS=Cucurbita moschata OX=3662 GN=LOC111435234 PE=... [more]
A0A6J1JGK12.4e-15493.46UPF0496 protein At3g19250-like OS=Cucurbita maxima OX=3661 GN=LOC111486700 PE=3 ... [more]
A0A5D3DUJ96.7e-9663.78UPF0496 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold236G0035... [more]
A0A1S3BP816.7e-9664.09UPF0496 protein At3g19330 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491707 PE=... [more]
A0A1S3BMN56.7e-9664.09UPF0496 protein At3g19330 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491707 PE=... [more]
Match NameE-valueIdentityDescription
AT3G19330.36.5e-4338.13Protein of unknown function (DUF677) [more]
AT3G19330.26.5e-4338.13Protein of unknown function (DUF677) [more]
AT3G19330.16.8e-4034.76Protein of unknown function (DUF677) [more]
AT3G19250.16.3e-3834.81Protein of unknown function (DUF677) [more]
AT3G49070.11.9e-1324.91Protein of unknown function (DUF677) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 252..272
NoneNo IPR availablePANTHERPTHR31113:SF5OS04G0405700 PROTEINcoord: 6..173
coord: 184..296
IPR007749Protein of unknown function DUF677PANTHERPTHR31113UPF0496 PROTEIN 3-RELATEDcoord: 6..173
coord: 184..296

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25303-RACarg25303-RAmRNA