Carg25215 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25215
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	cytochrome P450 87A3-like
Location	Carg_Chr16: 8080801 .. 8083321 (+)
RNA-Seq Expression	Carg25215
Synteny	Carg25215

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGCTTCTTTTGATTGGAGCTATTGTTGTTGTAAGTTTTACACACTGGCTTTATAGATGGAGAAATCCCAAATGTAATGGAAAACTTCCACCAGGTTCAATGGGTTTTCCTCTACTTGGTGAAACCCTGCAGTTCTTTGCTCCCAATACCTCCTCTGATATTCCTCCTTTCGTCAGAAACAGAATGGATAGGTTTGTTGTGTTCCTTCTTGTTCTTTTTCTTTCTCCTTATGTTCTTATTGAACGCGCTTAAATGTTTCAGGTATGGACCGATATTCCGAACGAGTTTGGTGGGAAGACCGTTGATTGTCTCAACAGACCCTGACTTGAATTACTATATCTTTCAAGAAGAGGGACAATTGTTCCAGAGCTGGTATCCTGATACCTTCACAGAGATATTTGGAAGGCAAAATGTTGGTTCGCTGCACGGTTTCATGTATAAATATCTTAAGAACATGGTTCTTCATCTTTTTGGCCCTGAAAGTCTGAGAAAGATGATACCTGAAGTTGAGGCTGTAGCAACTCAAAGATTGAGACAATGGTCATCTCAAAACTCAGTTGAATTGAAGGATAATACTGCAAGTGTAATTACATACTCGAACATCCTTGTTAACAATGGCATGTTTAGAAATTCTGTTCATTTTCCTTGTCTGATACTGATTTTTTGGTCCATGCAGATGATTTTTCATCTGACTGCAAAGAAGCTGATTAGTTATGATTCAGAAAATTCTTCGGAGAATTTGAGAGAGAACTTTGTTGCATTCATACAGGGCTTGATCTCCTTCCCTCTAAATGTTCCCGGAACTGCTTATAACAAATGCTTACAGGTAATCCAAATATAGTGTAGAAATTCTAATGATGCGCACTTCCTCTGTATTCATTTTTGCCATAGGAATTGAGGGTGCTCTTATGGATAACTTCAATTGGCAAAATTTTAATTCAGAACTGCAAGATGAACTTTGGAGTTCTTTAGAATAATTCAAAAGAAATTCCGTTTTCAGTTCAAAATTAAGCTTGTTTAGTAATAATTTCTTTATTTCATAATATATTCCTAATATACACAAGAAATAATTGCCAAATTTGGTATCAAAAGCTACATCTAAAAAGTTACTTTTTTTTAAGCTTCTACAATATGGCTAGTTTAATGAACATGTAGTTACAATATCGGTTAGTTTAGAGCCTTAATTTTTTAAAACACGATTGATATCAAATTATTAGTTTGAATATATCTTTCTGTTATCTTTGAAAATGTCTTACTTCTTATAATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAATATGTGTATATATATATACATATATTTATTTATTTATTTATACTATTTTCCCAACATTTGTTGTGTTTTGATATCAATATGACTTCAATTTGTTCCGTTCAAACCTACTAACAACACAGGGTACAATTGATAATGACTTGTTTTTTAACTAATCTATTGTAAAATTGATCTTTTACTTTGAAATGAATTAACAGGGTCGGAAAAGAGCGATGAGTATGCTGAAGAACATGTTACAAGAAAGGCGAGCAAATCCAAGGAAACAACAAATAGATTTTTTTGATTTTGTTCTTGAAGAACTGGAGAAAGATGGAACTCTTCTCACTGAGGAAATTGCTTTGGACCTTATGTTTGTACTACTTTTTGCAAGCTTTGAAACTACTTCACTTGCTATTACAGCAGCCATCAAATTTCTCCTCAACAATCCTCATGTGCTCGAGGAGCTGACGGTCCGTTCTGTTCTATTATAGCAGCCATAAAAGCTACTTGCTTTCCACAACCTTGTTGAAATCTGTCCTTTTCTGATGAGTATTTGCATCTTTATCAGGCTGAACATGAGGGAATATTGAGGAGAAGGGAAAATGCACATTCTGGGCTTACTTGGGAGGAATACAAATCCATGACATTCACATTTCAGGTTACTTGAATTATTCACATGTTCATTCTTTTCTCTCTTTTATGCCCTATGTTTCTCACAAACCAACTTCATCAAATTTCAGTTTATCAACGAAACGGCGAGACTGGCAAATATAGTTCCAGGAATCTTTCGAAAAGCACTTAGAGATATCCAGTTTAAGGGTATGCAGTAGTAGCATTTAAGATGTTAGATTTTTTTTTTTTTTTTTTCCTGCAAAAGATCATCCCAGATGTTGACTAAAAATGTATGCACCTTAATTCGAGCAGGATATACCATTCCAGCAGGCTGGGCTGTTATGGTCTGCCCTCCAGCTGTGCACTTAAACCCGGAAAAGTACATCGATCCCCTTGCTTTTAACCCGTGGCGATGGGAGGTATGTTGAGGATACATCGTTAGTCCTTTTTTTGTTGTCTGAGCTAGTTACTATTGTGAGTAGTTTCACTACGTTTGATATTTGGGATCACAATAATTGATTTTCCTTTTTGCCAGAAATCTGAACTGAAGGGAGCTTCTAAACATTTCATGGCTTTTGGTGGGGGCATGAGGTTCTGTGTTGGTACAGATTTTACCAAAGTTCAAATGGCT

mRNA sequence

ATGTGGCTTCTTTTGATTGGAGCTATTGTTGTTGTAAGTTTTACACACTGGCTTTATAGATGGAGAAATCCCAAATGTAATGGAAAACTTCCACCAGGTTCAATGGGTTTTCCTCTACTTGGTGAAACCCTGCAGTTCTTTGCTCCCAATACCTCCTCTGATATTCCTCCTTTCGTCAGAAACAGAATGGATAGGTATGGACCGATATTCCGAACGAGTTTGGTGGGAAGACCGTTGATTGTCTCAACAGACCCTGACTTGAATTACTATATCTTTCAAGAAGAGGGACAATTGTTCCAGAGCTGGTATCCTGATACCTTCACAGAGATATTTGGAAGGCAAAATGTTGGTTCGCTGCACGGTTTCATGTATAAATATCTTAAGAACATGGTTCTTCATCTTTTTGGCCCTGAAAGTCTGAGAAAGATGATACCTGAAGTTGAGGCTGTAGCAACTCAAAGATTGAGACAATGGTCATCTCAAAACTCAGTTGAATTGAAGGATAATACTGCAAGTATGATTTTTCATCTGACTGCAAAGAAGCTGATTAGTTATGATTCAGAAAATTCTTCGGAGAATTTGAGAGAGAACTTTGTTGCATTCATACAGGGCTTGATCTCCTTCCCTCTAAATGTTCCCGGAACTGCTTATAACAAATGCTTACAGGGTCGGAAAAGAGCGATGAGTATGCTGAAGAACATGTTACAAGAAAGGCGAGCAAATCCAAGGAAACAACAAATAGATTTTTTTGATTTTGTTCTTGAAGAACTGGAGAAAGATGGAACTCTTCTCACTGAGGAAATTGCTTTGGACCTTATGTTTGTACTACTTTTTGCAAGCTTTGAAACTACTTCACTTGCTATTACAGCAGCCATCAAATTTCTCCTCAACAATCCTCATGTGCTCGAGGAGCTGACGGCTGAACATGAGGGAATATTGAGGAGAAGGGAAAATGCACATTCTGGGCTTACTTGGGAGGAATACAAATCCATGACATTCACATTTCAGTTTATCAACGAAACGGCGAGACTGGCAAATATAGTTCCAGGAATCTTTCGAAAAGCACTTAGAGATATCCAGTTTAAGGGATATACCATTCCAGCAGGCTGGGCTGTTATGGTCTGCCCTCCAGCTGTGCACTTAAACCCGGAAAAGTACATCGATCCCCTTGCTTTTAACCCGTGGCGATGGGAGAAATCTGAACTGAAGGGAGCTTCTAAACATTTCATGGCTTTTGGTGGGGGCATGAGGTTCTGTGTTGGTACAGATTTTACCAAAGTTCAAATGGCT

Coding sequence (CDS)

ATGTGGCTTCTTTTGATTGGAGCTATTGTTGTTGTAAGTTTTACACACTGGCTTTATAGATGGAGAAATCCCAAATGTAATGGAAAACTTCCACCAGGTTCAATGGGTTTTCCTCTACTTGGTGAAACCCTGCAGTTCTTTGCTCCCAATACCTCCTCTGATATTCCTCCTTTCGTCAGAAACAGAATGGATAGGTATGGACCGATATTCCGAACGAGTTTGGTGGGAAGACCGTTGATTGTCTCAACAGACCCTGACTTGAATTACTATATCTTTCAAGAAGAGGGACAATTGTTCCAGAGCTGGTATCCTGATACCTTCACAGAGATATTTGGAAGGCAAAATGTTGGTTCGCTGCACGGTTTCATGTATAAATATCTTAAGAACATGGTTCTTCATCTTTTTGGCCCTGAAAGTCTGAGAAAGATGATACCTGAAGTTGAGGCTGTAGCAACTCAAAGATTGAGACAATGGTCATCTCAAAACTCAGTTGAATTGAAGGATAATACTGCAAGTATGATTTTTCATCTGACTGCAAAGAAGCTGATTAGTTATGATTCAGAAAATTCTTCGGAGAATTTGAGAGAGAACTTTGTTGCATTCATACAGGGCTTGATCTCCTTCCCTCTAAATGTTCCCGGAACTGCTTATAACAAATGCTTACAGGGTCGGAAAAGAGCGATGAGTATGCTGAAGAACATGTTACAAGAAAGGCGAGCAAATCCAAGGAAACAACAAATAGATTTTTTTGATTTTGTTCTTGAAGAACTGGAGAAAGATGGAACTCTTCTCACTGAGGAAATTGCTTTGGACCTTATGTTTGTACTACTTTTTGCAAGCTTTGAAACTACTTCACTTGCTATTACAGCAGCCATCAAATTTCTCCTCAACAATCCTCATGTGCTCGAGGAGCTGACGGCTGAACATGAGGGAATATTGAGGAGAAGGGAAAATGCACATTCTGGGCTTACTTGGGAGGAATACAAATCCATGACATTCACATTTCAGTTTATCAACGAAACGGCGAGACTGGCAAATATAGTTCCAGGAATCTTTCGAAAAGCACTTAGAGATATCCAGTTTAAGGGATATACCATTCCAGCAGGCTGGGCTGTTATGGTCTGCCCTCCAGCTGTGCACTTAAACCCGGAAAAGTACATCGATCCCCTTGCTTTTAACCCGTGGCGATGGGAGAAATCTGAACTGAAGGGAGCTTCTAAACATTTCATGGCTTTTGGTGGGGGCATGAGGTTCTGTGTTGGTACAGATTTTACCAAAGTTCAAATGGCT

Protein sequence

MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAKKLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERRANPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHVLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQFKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCVGTDFTKVQMA

Homology

[Close]

BLAST of Carg25215 vs. NCBI nr
Match: KAG7015620.1 (Cytochrome P450 87A3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 880.9 bits (2275), Expect = 4.3e-252
Identity = 430/430 (100.00%), Postives = 430/430 (100.00%), Query Frame = 0


Query: 1   MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR 60
           MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR
Sbjct: 1   MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR 60

Query: 61  NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH 120
           NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH
Sbjct: 61  NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH 120

Query: 121 GFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAK 180
           GFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAK
Sbjct: 121 GFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAK 180

Query: 181 KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERRA 240
           KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERRA
Sbjct: 181 KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERRA 240

Query: 241 NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300
           NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH
Sbjct: 241 NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ 360
           VLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ
Sbjct: 301 VLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ 360

Query: 361 FKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV 420
           FKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV
Sbjct: 361 FKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV 420

Query: 421 GTDFTKVQMA 431
           GTDFTKVQMA
Sbjct: 421 GTDFTKVQMA 430

[Close]

BLAST of Carg25215 vs. NCBI nr
Match: XP_022923566.1 (cytochrome P450 87A3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 879.0 bits (2270), Expect = 1.6e-251
Identity = 429/430 (99.77%), Postives = 429/430 (99.77%), Query Frame = 0


Query: 1   MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR 60
           MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR
Sbjct: 1   MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR 60

Query: 61  NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH 120
           NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH
Sbjct: 61  NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH 120

Query: 121 GFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAK 180
           GFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAK
Sbjct: 121 GFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAK 180

Query: 181 KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERRA 240
           KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAM MLKNMLQERRA
Sbjct: 181 KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300
           NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH
Sbjct: 241 NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ 360
           VLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ
Sbjct: 301 VLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ 360

Query: 361 FKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV 420
           FKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV
Sbjct: 361 FKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV 420

Query: 421 GTDFTKVQMA 431
           GTDFTKVQMA
Sbjct: 421 GTDFTKVQMA 430

[Close]

BLAST of Carg25215 vs. NCBI nr
Match: XP_023553524.1 (cytochrome P450 87A3-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 878.6 bits (2269), Expect = 2.1e-251
Identity = 428/430 (99.53%), Postives = 429/430 (99.77%), Query Frame = 0


Query: 1   MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR 60
           MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR
Sbjct: 1   MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR 60

Query: 61  NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH 120
           NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH
Sbjct: 61  NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH 120

Query: 121 GFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAK 180
           GFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAK
Sbjct: 121 GFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAK 180

Query: 181 KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERRA 240
           KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAM MLKNMLQERRA
Sbjct: 181 KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300
           NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH
Sbjct: 241 NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ 360
           VLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ
Sbjct: 301 VLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ 360

Query: 361 FKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV 420
           FKGYTIPAGWAVMVCPPAVHLNPEKY+DPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV
Sbjct: 361 FKGYTIPAGWAVMVCPPAVHLNPEKYVDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV 420

Query: 421 GTDFTKVQMA 431
           GTDFTKVQMA
Sbjct: 421 GTDFTKVQMA 430

[Close]

BLAST of Carg25215 vs. NCBI nr
Match: XP_023007812.1 (cytochrome P450 87A3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 873.2 bits (2255), Expect = 9.0e-250
Identity = 425/430 (98.84%), Postives = 427/430 (99.30%), Query Frame = 0


Query: 1   MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR 60
           MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR
Sbjct: 1   MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR 60

Query: 61  NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH 120
           NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH
Sbjct: 61  NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH 120

Query: 121 GFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAK 180
           GFMYKYLKNMVLHLFGPESLRKMIPEVEAVA QRLRQWSSQNSVELKDNTASMIFHLTAK
Sbjct: 121 GFMYKYLKNMVLHLFGPESLRKMIPEVEAVANQRLRQWSSQNSVELKDNTASMIFHLTAK 180

Query: 181 KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERRA 240
           KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAM MLKNMLQERRA
Sbjct: 181 KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300
           NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH
Sbjct: 241 NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ 360
           VLEEL AEHEGIL+RRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ
Sbjct: 301 VLEELMAEHEGILKRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ 360

Query: 361 FKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV 420
           FKGYTIPAGWAVMVCPPAVHLNPEKY+DPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV
Sbjct: 361 FKGYTIPAGWAVMVCPPAVHLNPEKYVDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV 420

Query: 421 GTDFTKVQMA 431
           GTDFTKVQMA
Sbjct: 421 GTDFTKVQMA 430

[Close]

BLAST of Carg25215 vs. NCBI nr
Match: XP_022923567.1 (cytochrome P450 87A3-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 858.2 bits (2216), Expect = 3.0e-245
Identity = 417/418 (99.76%), Postives = 417/418 (99.76%), Query Frame = 0


Query: 13  SFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNRMDRYGPIFRT 72
           SFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNRMDRYGPIFRT
Sbjct: 5   SFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNRMDRYGPIFRT 64

Query: 73  SLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGFMYKYLKNMVL 132
           SLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGFMYKYLKNMVL
Sbjct: 65  SLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGFMYKYLKNMVL 124

Query: 133 HLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAKKLISYDSENSSE 192
           HLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAKKLISYDSENSSE
Sbjct: 125 HLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAKKLISYDSENSSE 184

Query: 193 NLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERRANPRKQQIDFFDF 252
           NLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAM MLKNMLQERRANPRKQQIDFFDF
Sbjct: 185 NLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMRMLKNMLQERRANPRKQQIDFFDF 244

Query: 253 VLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHVLEELTAEHEGI 312
           VLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHVLEELTAEHEGI
Sbjct: 245 VLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHVLEELTAEHEGI 304

Query: 313 LRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQFKGYTIPAGWAV 372
           LRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQFKGYTIPAGWAV
Sbjct: 305 LRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQFKGYTIPAGWAV 364

Query: 373 MVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCVGTDFTKVQMA 431
           MVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCVGTDFTKVQMA
Sbjct: 365 MVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCVGTDFTKVQMA 422

[Close]

BLAST of Carg25215 vs. ExPASy Swiss-Prot
Match: Q7XU38 (Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV=3)

HSP 1 Score: 585.1 bits (1507), Expect = 6.4e-166
Identity = 276/431 (64.04%), Postives = 343/431 (79.58%), Query Frame = 0


Query: 3   LLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNR 62
           LL      VV+   W YRW +P+ NG+LPPGS+G P++GETLQFFAPN + D+ PFV+ R
Sbjct: 44  LLCAALAAVVALLRWAYRWSHPRSNGRLPPGSLGLPVIGETLQFFAPNPTCDLSPFVKER 103

Query: 63  MDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGF 122
           + RYG IF+TS+VGRP++VS DP++NYY+FQ+EG+LF+SWYPDTFTEIFGR NVGSLHGF
Sbjct: 104 IKRYGSIFKTSVVGRPVVVSADPEMNYYVFQQEGKLFESWYPDTFTEIFGRDNVGSLHGF 163

Query: 123 MYKYLKNMVLHLFGPESLRK-MIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAKK 182
           MYKYLK +VL L+G E+L+  ++ E +A     L  W+SQ SVELK+  ++MIF LTAKK
Sbjct: 164 MYKYLKTLVLRLYGQENLKSVLLAETDAACRGSLASWASQPSVELKEGISTMIFDLTAKK 223

Query: 183 LISYD-SENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERRA 242
           LI YD S+ S  NLR+NF AFI GLISFPLN+PGTAY++C++GRK AM +L+ M++ER A
Sbjct: 224 LIGYDPSKPSQVNLRKNFGAFICGLISFPLNIPGTAYHECMEGRKNAMKVLRGMMKERMA 283

Query: 243 NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 302
            P +   DFFD V++EL ++  LLTE IALDLMFVLLFASFETT+LA+T  +K L  NP 
Sbjct: 284 EPERPCEDFFDHVIQELRREKPLLTETIALDLMFVLLFASFETTALALTIGVKLLTENPK 343

Query: 303 VLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ 362
           V++ L  EHE I+R R++ +SG+TW EYKSMTFT Q I E  RLANIVPGIFRKAL+D++
Sbjct: 344 VVDALREEHEAIIRNRKDPNSGVTWAEYKSMTFTSQVIMEIVRLANIVPGIFRKALQDVE 403

Query: 363 FKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWE-KSELKGASKHFMAFGGGMRFC 422
            KGYTIPAGW +MVCPPAVHLNPE Y DPLAFNPWRW+ K E+ G +KHFMAFGGG+RFC
Sbjct: 404 IKGYTIPAGWGIMVCPPAVHLNPEIYEDPLAFNPWRWQGKPEITGGTKHFMAFGGGLRFC 463

Query: 423 VGTDFTKVQMA 431
           VGTD +KV MA
Sbjct: 464 VGTDLSKVLMA 474

[Close]

BLAST of Carg25215 vs. ExPASy Swiss-Prot
Match: K7NBR2 (Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=1 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 3.1e-104
Identity = 179/433 (41.34%), Postives = 273/433 (63.05%), Query Frame = 0


Query: 1   MWLLLIG--AIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPF 60
           MW +++G   + V  + HW+ +WR+ K NG LPPG+MG PL+GET+Q   P+ S D+ PF
Sbjct: 1   MWTVVLGLATLFVAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  VRNRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVG- 120
           ++ +++RYGPIF+T L GRP++VS D + N YI  +EG+  + WY DT ++ FG      
Sbjct: 61  IQKKVERYGPIFKTCLAGRPVVVSADAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 SLHGFMYKYLKNMVLHLFGPESLR-KMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFH 180
              G ++KY++++ L+ FG E+LR + +P +EA + + L  WS+Q SVE+K+ +A M+F 
Sbjct: 121 KALGLIHKYIRSITLNHFGAEALRERFLPFIEASSMEALHSWSTQPSVEVKNASALMVFR 180

Query: 181 LTAKKLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQ 240
            +  K+   D++  S N+   F   + G +S PLN PGT Y+KCL+  K     L+ ++ 
Sbjct: 181 TSVNKMFGEDAKKLSGNIPGKFTKLLGGFLSLPLNFPGTTYHKCLKDMKEIQKKLREVVD 240

Query: 241 ERRANPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLL 300
           +R AN      DF    L++ E +   ++EE  + L+F + FASFE+ S  +T  +K L 
Sbjct: 241 DRLANVGPDVEDFLGQALKDKESE-KFISEEFIIQLLFSISFASFESISTTLTLILKLLD 300

Query: 301 NNPHVLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKAL 360
            +P V++EL AEHE I + R +    +TWEEYKSMTFT Q INET RL ++ P + RK +
Sbjct: 301 EHPEVVKELEAEHEAIRKARADPDGPITWEEYKSMTFTLQVINETLRLGSVTPALLRKTV 360

Query: 361 RDIQFKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGM 420
           +D+Q KGY IP GW +M+   + H +P+ Y DP  FNPWRW+  +     K+FM FGGG+
Sbjct: 361 KDLQVKGYIIPEGWTIMLVTASRHRDPKVYKDPHIFNPWRWKDLDSITIQKNFMPFGGGL 420

Query: 421 RFCVGTDFTKVQM 430
           R C G +++KV +
Sbjct: 421 RHCAGAEYSKVYL 432

[Close]

BLAST of Carg25215 vs. ExPASy Swiss-Prot
Match: Q8L7D5 (Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3)

HSP 1 Score: 365.2 bits (936), Expect = 1.0e-99
Identity = 185/433 (42.73%), Postives = 274/433 (63.28%), Query Frame = 0


Query: 1   MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR 60
           +W++ + A+V+   + WLYRW NPKCNGKLPPGSMG P++GET  FF P+   +I PFV+
Sbjct: 9   VWVIAVAAVVI---SKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVK 68

Query: 61  NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH 120
            RM +YGP+FRT++ G   +V T+PD+ + +F++E + F   YP+ F + FG++NV   H
Sbjct: 69  KRMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKH 128

Query: 121 GFMYKYLKNMVLHLFGPESL-RKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTA 180
           G ++K++K + L   G E+L +KMI E++ V  + LR  ++Q S + K+   S+I     
Sbjct: 129 GNIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLT 188

Query: 181 KKLISYDSENSSENLRENFVAFIQGLISFPLNVPG-TAYNKCLQGRKRAMSMLKNMLQER 240
            K+IS     +   L +N +A        PL +    +  K    R+ A+ ++K++   R
Sbjct: 189 PKIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRR 248

Query: 241 RANPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNN 300
           +A+ R+   DF D ++EE EK+  +  EE A++L+F +L  + E+TS   + AIKFL  N
Sbjct: 249 KAS-REMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAEN 308

Query: 301 PHVLEELTAEHEGILRRRENAHSGLTWEEYK-SMTFTFQFINETARLANIVPGIFRKALR 360
              L EL  EH  IL+ R    +G++WEEY+  MTFT   INET R+AN+ P ++RKA+ 
Sbjct: 309 HKALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETLRMANMAPIMYRKAVN 368

Query: 361 DIQFKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMR 420
           D++ KGYTIPAGW V V PPAVH N   Y +PL FNPWRWE  EL+  SK FM FGGG+R
Sbjct: 369 DVEIKGYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWRWEGKELRSGSKTFMVFGGGVR 428

Query: 421 FCVGTDFTKVQMA 431
            CVG +F ++Q++
Sbjct: 429 QCVGAEFARLQIS 437

[Close]

BLAST of Carg25215 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 291.2 bits (744), Expect = 1.9e-77
Identity = 172/466 (36.91%), Postives = 260/466 (55.79%), Query Frame = 0


Query: 3   LLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNR 62
           LLL+ +++ +     L + RN K    LPPG  G+P LGET+ +  P T++ +  F++  
Sbjct: 12  LLLLPSLLSLLLFLILLKRRNRKTRFNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQH 71

Query: 63  MDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGF 122
           + +YG I+R++L G P IVS D  LN +I Q EG+LF+  YP +   I G+ ++  L G 
Sbjct: 72  VSKYGKIYRSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGD 131

Query: 123 MYKYLKNMVLHLFGPESLRK-MIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAKK 182
           M++ ++++ L+      LR  ++ +VE      L  W   +    +D      F+L AK 
Sbjct: 132 MHRDMRSISLNFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKH 191

Query: 183 LISYD-SENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERRA 242
           ++S D  E  +E L++ +V F++G++S PLN+PGTAY+K LQ R   +  ++  ++ER+ 
Sbjct: 192 IMSMDPGEEETEQLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKL 251

Query: 243 ------------------------NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVL 302
                                   + RKQ+ D  D +L  + K   L TE+I LDL+  L
Sbjct: 252 DIKEEDQEEEEVKTEDEAEMSKSDHVRKQRTD--DDLLGWVLKHSNLSTEQI-LDLILSL 311

Query: 303 LFASFETTSLAITAAIKFLLNNPHVLEELTAEHEGILR-RRENAHSGLTWEEYKSMTFTF 362
           LFA  ET+S+AI  AI FL   P  +EEL  EH  I R ++E   S L W++YK M FT 
Sbjct: 312 LFAGHETSSVAIALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQ 371

Query: 363 QFINETARLANIVPGIFRKALRDIQFKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPW 422
             INET RL N+V  + RKAL+D+++KGY IP+GW V+    AVHL+  +Y  P  FNPW
Sbjct: 372 CVINETLRLGNVVRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPW 431

Query: 423 RWEKSELKGAS-----------KHFMAFGGGMRFCVGTDFTKVQMA 431
           RW++    GAS            ++M FGGG R C G++  K++MA
Sbjct: 432 RWQQQN-NGASSSGSGSFSTWGNNYMPFGGGPRLCAGSELAKLEMA 473

[Close]

BLAST of Carg25215 vs. ExPASy Swiss-Prot
Match: Q5CCK3 (Cytochrome P450 90B2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90B2 PE=1 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 6.2e-76
Identity = 160/431 (37.12%), Postives = 241/431 (55.92%), Query Frame = 0


Query: 16  HWLYRWRNPKCNGK-----LPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNRMDRYGPIF 75
           HW   WR      K     LPPG+ G+PL+GET  +   + ++ +  F+   + RYG I+
Sbjct: 38  HW---WRGGTTPAKRKRMNLPPGAAGWPLVGETFGYLRAHPATSVGRFMEQHIARYGKIY 97

Query: 76  RTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGFMYKYLKNM 135
           R+SL G   +VS D  LN YI Q EG+LF+  YP +   I G+ ++  L G  ++ ++ +
Sbjct: 98  RSSLFGERTVVSADAGLNRYILQNEGRLFECSYPRSIGGILGKWSMLVLVGDPHREMRAI 157

Query: 136 VLHLFGPESLRK-MIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAKKLISYD-SE 195
            L+      LR  ++PEVE      LR W   ++   +       F+L AK ++S D  E
Sbjct: 158 SLNFLSSVRLRAVLLPEVERHTLLVLRAWPPSSTFSAQHQAKKFTFNLMAKNIMSMDPGE 217

Query: 196 NSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQER-----RANPR 255
             +E LR  ++ F++G++S PLN+PGT Y K L+ R   + +++  ++ER     + +  
Sbjct: 218 EETERLRREYITFMKGVVSAPLNLPGTPYWKALKSRAAILGVIERKMEERVEKLSKEDAS 277

Query: 256 KQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHVLE 315
            +Q D   + L++     + L++E  LDL+  LLFA  ET+S+A+  AI FL   P  ++
Sbjct: 278 VEQDDLLGWALKQ-----SNLSKEQILDLLLSLLFAGHETSSMALALAIFFLEGCPKAVQ 337

Query: 316 ELTAEHEGILRR-RENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQFK 375
           EL  EH GI RR R      L+WE+YK M FT   INET RL N+V  + RK ++D+ +K
Sbjct: 338 ELREEHLGIARRQRLRGECKLSWEDYKEMVFTQCVINETLRLGNVVRFLHRKVIKDVHYK 397

Query: 376 GYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGA---SKHFMAFGGGMRFC 431
           GY IP+GW ++    AVHL+   Y DP  FNPWRW+ S   G    S  FM +GGG R C
Sbjct: 398 GYDIPSGWKILPVLAAVHLDSSLYEDPQRFNPWRWKSSGSSGGLAQSSSFMPYGGGTRLC 457

[Close]

BLAST of Carg25215 vs. ExPASy TrEMBL
Match: A0A6J1E749 (cytochrome P450 87A3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431215 PE=3 SV=1)

HSP 1 Score: 879.0 bits (2270), Expect = 8.0e-252
Identity = 429/430 (99.77%), Postives = 429/430 (99.77%), Query Frame = 0


Query: 1   MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR 60
           MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR
Sbjct: 1   MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR 60

Query: 61  NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH 120
           NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH
Sbjct: 61  NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH 120

Query: 121 GFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAK 180
           GFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAK
Sbjct: 121 GFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAK 180

Query: 181 KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERRA 240
           KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAM MLKNMLQERRA
Sbjct: 181 KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300
           NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH
Sbjct: 241 NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ 360
           VLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ
Sbjct: 301 VLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ 360

Query: 361 FKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV 420
           FKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV
Sbjct: 361 FKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV 420

Query: 421 GTDFTKVQMA 431
           GTDFTKVQMA
Sbjct: 421 GTDFTKVQMA 430

[Close]

BLAST of Carg25215 vs. ExPASy TrEMBL
Match: A0A6J1KZQ5 (cytochrome P450 87A3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500308 PE=3 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 4.4e-250
Identity = 425/430 (98.84%), Postives = 427/430 (99.30%), Query Frame = 0


Query: 1   MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR 60
           MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR
Sbjct: 1   MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR 60

Query: 61  NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH 120
           NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH
Sbjct: 61  NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH 120

Query: 121 GFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAK 180
           GFMYKYLKNMVLHLFGPESLRKMIPEVEAVA QRLRQWSSQNSVELKDNTASMIFHLTAK
Sbjct: 121 GFMYKYLKNMVLHLFGPESLRKMIPEVEAVANQRLRQWSSQNSVELKDNTASMIFHLTAK 180

Query: 181 KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERRA 240
           KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAM MLKNMLQERRA
Sbjct: 181 KLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300
           NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH
Sbjct: 241 NPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ 360
           VLEEL AEHEGIL+RRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ
Sbjct: 301 VLEELMAEHEGILKRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQ 360

Query: 361 FKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV 420
           FKGYTIPAGWAVMVCPPAVHLNPEKY+DPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV
Sbjct: 361 FKGYTIPAGWAVMVCPPAVHLNPEKYVDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV 420

Query: 421 GTDFTKVQMA 431
           GTDFTKVQMA
Sbjct: 421 GTDFTKVQMA 430

[Close]

BLAST of Carg25215 vs. ExPASy TrEMBL
Match: A0A6J1E6G8 (cytochrome P450 87A3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431215 PE=3 SV=1)

HSP 1 Score: 858.2 bits (2216), Expect = 1.5e-245
Identity = 417/418 (99.76%), Postives = 417/418 (99.76%), Query Frame = 0


Query: 13  SFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNRMDRYGPIFRT 72
           SFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNRMDRYGPIFRT
Sbjct: 5   SFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNRMDRYGPIFRT 64

Query: 73  SLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGFMYKYLKNMVL 132
           SLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGFMYKYLKNMVL
Sbjct: 65  SLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGFMYKYLKNMVL 124

Query: 133 HLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAKKLISYDSENSSE 192
           HLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAKKLISYDSENSSE
Sbjct: 125 HLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAKKLISYDSENSSE 184

Query: 193 NLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERRANPRKQQIDFFDF 252
           NLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAM MLKNMLQERRANPRKQQIDFFDF
Sbjct: 185 NLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMRMLKNMLQERRANPRKQQIDFFDF 244

Query: 253 VLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHVLEELTAEHEGI 312
           VLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHVLEELTAEHEGI
Sbjct: 245 VLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHVLEELTAEHEGI 304

Query: 313 LRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQFKGYTIPAGWAV 372
           LRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQFKGYTIPAGWAV
Sbjct: 305 LRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQFKGYTIPAGWAV 364

Query: 373 MVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCVGTDFTKVQMA 431
           MVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCVGTDFTKVQMA
Sbjct: 365 MVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCVGTDFTKVQMA 422

[Close]

BLAST of Carg25215 vs. ExPASy TrEMBL
Match: A0A6J1L8P6 (cytochrome P450 87A3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111500308 PE=3 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 8.0e-244
Identity = 413/418 (98.80%), Postives = 415/418 (99.28%), Query Frame = 0


Query: 13  SFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNRMDRYGPIFRT 72
           SFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNRMDRYGPIFRT
Sbjct: 5   SFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNRMDRYGPIFRT 64

Query: 73  SLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGFMYKYLKNMVL 132
           SLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGFMYKYLKNMVL
Sbjct: 65  SLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGFMYKYLKNMVL 124

Query: 133 HLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAKKLISYDSENSSE 192
           HLFGPESLRKMIPEVEAVA QRLRQWSSQNSVELKDNTASMIFHLTAKKLISYDSENSSE
Sbjct: 125 HLFGPESLRKMIPEVEAVANQRLRQWSSQNSVELKDNTASMIFHLTAKKLISYDSENSSE 184

Query: 193 NLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERRANPRKQQIDFFDF 252
           NLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAM MLKNMLQERRANPRKQQIDFFDF
Sbjct: 185 NLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMRMLKNMLQERRANPRKQQIDFFDF 244

Query: 253 VLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHVLEELTAEHEGI 312
           VLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHVLEEL AEHEGI
Sbjct: 245 VLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHVLEELMAEHEGI 304

Query: 313 LRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQFKGYTIPAGWAV 372
           L+RRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQFKGYTIPAGWAV
Sbjct: 305 LKRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQFKGYTIPAGWAV 364

Query: 373 MVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCVGTDFTKVQMA 431
           MVCPPAVHLNPEKY+DPLAFNPWRWEKSELKGASKHFMAFGGGMRFCVGTDFTKVQMA
Sbjct: 365 MVCPPAVHLNPEKYVDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCVGTDFTKVQMA 422

[Close]

BLAST of Carg25215 vs. ExPASy TrEMBL
Match: A0A6J1EA00 (cytochrome P450 87A3-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111431215 PE=3 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 3.1e-240
Identity = 410/411 (99.76%), Postives = 410/411 (99.76%), Query Frame = 0


Query: 20  RWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNRMDRYGPIFRTSLVGRPL 79
           RWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNRMDRYGPIFRTSLVGRPL
Sbjct: 5   RWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNRMDRYGPIFRTSLVGRPL 64

Query: 80  IVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGFMYKYLKNMVLHLFGPES 139
           IVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGFMYKYLKNMVLHLFGPES
Sbjct: 65  IVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGFMYKYLKNMVLHLFGPES 124

Query: 140 LRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAKKLISYDSENSSENLRENFV 199
           LRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAKKLISYDSENSSENLRENFV
Sbjct: 125 LRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAKKLISYDSENSSENLRENFV 184

Query: 200 AFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERRANPRKQQIDFFDFVLEELEK 259
           AFIQGLISFPLNVPGTAYNKCLQGRKRAM MLKNMLQERRANPRKQQIDFFDFVLEELEK
Sbjct: 185 AFIQGLISFPLNVPGTAYNKCLQGRKRAMRMLKNMLQERRANPRKQQIDFFDFVLEELEK 244

Query: 260 DGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHVLEELTAEHEGILRRRENA 319
           DGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHVLEELTAEHEGILRRRENA
Sbjct: 245 DGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHVLEELTAEHEGILRRRENA 304

Query: 320 HSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQFKGYTIPAGWAVMVCPPAV 379
           HSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQFKGYTIPAGWAVMVCPPAV
Sbjct: 305 HSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDIQFKGYTIPAGWAVMVCPPAV 364

Query: 380 HLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCVGTDFTKVQMA 431
           HLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCVGTDFTKVQMA
Sbjct: 365 HLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCVGTDFTKVQMA 415

[Close]

BLAST of Carg25215 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 691.0 bits (1782), Expect = 5.9e-199
Identity = 327/431 (75.87%), Postives = 380/431 (88.17%), Query Frame = 0


Query: 1   MWLLLIG-AIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFV 60
           MW LLI  +++++S THW+Y WRNPKC GKLPPGSMGFPLLGE++QFF PN +SDIPPF+
Sbjct: 1   MWALLIWVSLLLISITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPFI 60

Query: 61  RNRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSL 120
           + R+ +YGPIF+T+LVGRP+IVSTD DL+Y++F +EG+ FQSWYPDTFT IFG++NVGSL
Sbjct: 61  KERVKKYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVGSL 120

Query: 121 HGFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTA 180
           HGFMYKYLKNMVL LFG + L+KM+P+VE  A +RL  WS+Q+SVELKD TASMIF LTA
Sbjct: 121 HGFMYKYLKNMVLTLFGHDGLKKMLPQVEMTANKRLELWSNQDSVELKDATASMIFDLTA 180

Query: 181 KKLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERR 240
           KKLIS+D + SSENLR NFVAFIQGLISFP ++PGTAY+KCLQGR +AM ML+NMLQERR
Sbjct: 181 KKLISHDPDKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNMLQERR 240

Query: 241 ANPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNP 300
            NPRK   DFFD+V+EE++K+GT+LTEEIALDLMFVLLFASFETTSLA+T AIKFL ++P
Sbjct: 241 ENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIKFLSDDP 300

Query: 301 HVLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFRKALRDI 360
            VL+ LT EHE ILR RE+A SGLTWEEYKSMT+TFQFINETARLANIVP IFRKALRDI
Sbjct: 301 EVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAIFRKALRDI 360

Query: 361 QFKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFC 420
           +FK YTIPAGWAVMVCPPAVHLNPE Y DPL FNP RWE S++  ASKHFMAFGGGMRFC
Sbjct: 361 KFKDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNASKHFMAFGGGMRFC 420

Query: 421 VGTDFTKVQMA 431
           VGTDFTK+QMA
Sbjct: 421 VGTDFTKLQMA 431

[Close]

BLAST of Carg25215 vs. TAIR 10
Match: AT1G12740.2 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 686.4 bits (1770), Expect = 1.5e-197
Identity = 329/437 (75.29%), Postives = 380/437 (86.96%), Query Frame = 0


Query: 1   MWLLLIG-AIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFV 60
           MW LLI  +++++S THW+Y WRNPKC GKLPPGSMGFPLLGE++QFF PN +SDIPPF+
Sbjct: 1   MWALLIWVSLLLISITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPFI 60

Query: 61  RNR------MDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGR 120
           + R      M RYGPIF+T+LVGRP+IVSTD DL+Y++F +EG+ FQSWYPDTFT IFG+
Sbjct: 61  KERVKNDVDMCRYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGK 120

Query: 121 QNVGSLHGFMYKYLKNMVLHLFGPESLRKMIPEVEAVATQRLRQWSSQNSVELKDNTASM 180
           +NVGSLHGFMYKYLKNMVL LFG + L+KM+P+VE  A +RL  WS+Q+SVELKD TASM
Sbjct: 121 KNVGSLHGFMYKYLKNMVLTLFGHDGLKKMLPQVEMTANKRLELWSNQDSVELKDATASM 180

Query: 181 IFHLTAKKLISYDSENSSENLRENFVAFIQGLISFPLNVPGTAYNKCLQGRKRAMSMLKN 240
           IF LTAKKLIS+D + SSENLR NFVAFIQGLISFP ++PGTAY+KCLQGR +AM ML+N
Sbjct: 181 IFDLTAKKLISHDPDKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRN 240

Query: 241 MLQERRANPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIK 300
           MLQERR NPRK   DFFD+V+EE++K+GT+LTEEIALDLMFVLLFASFETTSLA+T AIK
Sbjct: 241 MLQERRENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIK 300

Query: 301 FLLNNPHVLEELTAEHEGILRRRENAHSGLTWEEYKSMTFTFQFINETARLANIVPGIFR 360
           FL ++P VL+ LT EHE ILR RE+A SGLTWEEYKSMT+TFQFINETARLANIVP IFR
Sbjct: 301 FLSDDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAIFR 360

Query: 361 KALRDIQFKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFG 420
           KALRDI+FK YTIPAGWAVMVCPPAVHLNPE Y DPL FNP RWE S++  ASKHFMAFG
Sbjct: 361 KALRDIKFKDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNASKHFMAFG 420

Query: 421 GGMRFCVGTDFTKVQMA 431
           GGMRFCVGTDFTK+QMA
Sbjct: 421 GGMRFCVGTDFTKLQMA 437

[Close]

BLAST of Carg25215 vs. TAIR 10
Match: AT1G78490.1 (cytochrome P450, family 708, subfamily A, polypeptide 3 )

HSP 1 Score: 377.1 bits (967), Expect = 1.9e-104
Identity = 190/430 (44.19%), Postives = 273/430 (63.49%), Query Frame = 0


Query: 4   LLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVRNRM 63
           +L+ A+VVV  +HWLYRW NPKC GKLPPGSMGFP++GETL FF P     IP FV+ RM
Sbjct: 9   MLMVALVVVRISHWLYRWSNPKCPGKLPPGSMGFPIIGETLDFFKPCGVEGIPTFVKKRM 68

Query: 64  DRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLHGFM 123
            RYGP+FRT++ G   +VSTDPD+ + IF++E   F+  YPD F ++FG+ N+     F+
Sbjct: 69  IRYGPLFRTNIFGSKTVVSTDPDVIHQIFRQENTSFELGYPDIFVKVFGKDNLFLKEVFI 128

Query: 124 YKYLKNMVLHLFGPESLRK-MIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTAKKL 183
           +KYL+ + + + G E L++ M+  ++      +R  +SQ S  ++    +++      KL
Sbjct: 129 HKYLQKITMQILGSEGLKQTMLGNMDKATRDHIRSIASQGSFNVRKEVENLVVAYMTPKL 188

Query: 184 ISYDSENSSENLRENFVAF-IQGLISFPLNVPGTAYNKCLQGRKRAMSMLKNMLQERRAN 243
           IS     +   L +N  AF +    SF       A  K L+ R+ A+ ++K++L  R+  
Sbjct: 189 ISNLKPETQSKLIDNLNAFNLDWFKSFLRLSTWKAVTKALKSREEAIQVMKDVLMMRKET 248

Query: 244 PRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPHV 303
             KQ+ DF + +LEELEKDG+   +  A++L+F+L FA  E TS     A+KF+  +P V
Sbjct: 249 REKQE-DFLNTLLEELEKDGSFFDQGSAINLIFLLAFALREGTSSCTALAVKFISKDPKV 308

Query: 304 LEELTAEHEGILRRRENAHSGLTWEEYK-SMTFTFQFINETARLANIVPGIFRKALRDIQ 363
           L EL  EH+ I+  R++  +G++WEEY+ +MTFT    NE  RLAN  P +FRKA++D++
Sbjct: 309 LAELKREHKAIVDNRKDKEAGVSWEEYRHNMTFTNMVSNEVLRLANTTPLLFRKAVQDVE 368

Query: 364 FKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMRFCV 423
            KGYTIPAGW V V P AVH +P  Y +P  FNPWRWE  E+   SK FMAFG G+R CV
Sbjct: 369 IKGYTIPAGWIVAVAPSAVHFDPAIYENPFEFNPWRWEGKEMIWGSKTFMAFGYGVRLCV 428

Query: 424 GTDFTKVQMA 431
           G +F+++QMA
Sbjct: 429 GAEFSRLQMA 437

[Close]

BLAST of Carg25215 vs. TAIR 10
Match: AT5G48000.2 (cytochrome P450, family 708, subfamily A, polypeptide 2 )

HSP 1 Score: 365.2 bits (936), Expect = 7.4e-101
Identity = 185/433 (42.73%), Postives = 274/433 (63.28%), Query Frame = 0


Query: 1   MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR 60
           +W++ + A+V+   + WLYRW NPKCNGKLPPGSMG P++GET  FF P+   +I PFV+
Sbjct: 9   VWVIAVAAVVI---SKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVK 68

Query: 61  NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH 120
            RM +YGP+FRT++ G   +V T+PD+ + +F++E + F   YP+ F + FG++NV   H
Sbjct: 69  KRMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKH 128

Query: 121 GFMYKYLKNMVLHLFGPESL-RKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTA 180
           G ++K++K + L   G E+L +KMI E++ V  + LR  ++Q S + K+   S+I     
Sbjct: 129 GNIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLT 188

Query: 181 KKLISYDSENSSENLRENFVAFIQGLISFPLNVPG-TAYNKCLQGRKRAMSMLKNMLQER 240
            K+IS     +   L +N +A        PL +    +  K    R+ A+ ++K++   R
Sbjct: 189 PKIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRR 248

Query: 241 RANPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNN 300
           +A+ R+   DF D ++EE EK+  +  EE A++L+F +L  + E+TS   + AIKFL  N
Sbjct: 249 KAS-REMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAEN 308

Query: 301 PHVLEELTAEHEGILRRRENAHSGLTWEEYK-SMTFTFQFINETARLANIVPGIFRKALR 360
              L EL  EH  IL+ R    +G++WEEY+  MTFT   INET R+AN+ P ++RKA+ 
Sbjct: 309 HKALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETLRMANMAPIMYRKAVN 368

Query: 361 DIQFKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMR 420
           D++ KGYTIPAGW V V PPAVH N   Y +PL FNPWRWE  EL+  SK FM FGGG+R
Sbjct: 369 DVEIKGYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWRWEGKELRSGSKTFMVFGGGVR 428

Query: 421 FCVGTDFTKVQMA 431
            CVG +F ++Q++
Sbjct: 429 QCVGAEFARLQIS 437

[Close]

BLAST of Carg25215 vs. TAIR 10
Match: AT5G48000.3 (cytochrome P450, family 708, subfamily A, polypeptide 2 )

HSP 1 Score: 365.2 bits (936), Expect = 7.4e-101
Identity = 185/433 (42.73%), Postives = 274/433 (63.28%), Query Frame = 0


Query: 1   MWLLLIGAIVVVSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDIPPFVR 60
           +W++ + A+V+   + WLYRW NPKCNGKLPPGSMG P++GET  FF P+   +I PFV+
Sbjct: 9   VWVIAVAAVVI---SKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVK 68

Query: 61  NRMDRYGPIFRTSLVGRPLIVSTDPDLNYYIFQEEGQLFQSWYPDTFTEIFGRQNVGSLH 120
            RM +YGP+FRT++ G   +V T+PD+ + +F++E + F   YP+ F + FG++NV   H
Sbjct: 69  KRMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKH 128

Query: 121 GFMYKYLKNMVLHLFGPESL-RKMIPEVEAVATQRLRQWSSQNSVELKDNTASMIFHLTA 180
           G ++K++K + L   G E+L +KMI E++ V  + LR  ++Q S + K+   S+I     
Sbjct: 129 GNIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLT 188

Query: 181 KKLISYDSENSSENLRENFVAFIQGLISFPLNVPG-TAYNKCLQGRKRAMSMLKNMLQER 240
            K+IS     +   L +N +A        PL +    +  K    R+ A+ ++K++   R
Sbjct: 189 PKIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRR 248

Query: 241 RANPRKQQIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNN 300
           +A+ R+   DF D ++EE EK+  +  EE A++L+F +L  + E+TS   + AIKFL  N
Sbjct: 249 KAS-REMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAEN 308

Query: 301 PHVLEELTAEHEGILRRRENAHSGLTWEEYK-SMTFTFQFINETARLANIVPGIFRKALR 360
              L EL  EH  IL+ R    +G++WEEY+  MTFT   INET R+AN+ P ++RKA+ 
Sbjct: 309 HKALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETLRMANMAPIMYRKAVN 368

Query: 361 DIQFKGYTIPAGWAVMVCPPAVHLNPEKYIDPLAFNPWRWEKSELKGASKHFMAFGGGMR 420
           D++ KGYTIPAGW V V PPAVH N   Y +PL FNPWRWE  EL+  SK FM FGGG+R
Sbjct: 369 DVEIKGYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWRWEGKELRSGSKTFMVFGGGVR 428

Query: 421 FCVGTDFTKVQMA 431
            CVG +F ++Q++
Sbjct: 429 QCVGAEFARLQIS 437

The following BLAST results are available for this feature:

BLAST of Carg25215 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7015620.1	4.3e-252	100.00	Cytochrome P450 87A3, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022923566.1	1.6e-251	99.77	cytochrome P450 87A3-like isoform X1 [Cucurbita moschata]	[more]
XP_023553524.1	2.1e-251	99.53	cytochrome P450 87A3-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_023007812.1	9.0e-250	98.84	cytochrome P450 87A3-like isoform X1 [Cucurbita maxima]	[more]
XP_022923567.1	3.0e-245	99.76	cytochrome P450 87A3-like isoform X2 [Cucurbita moschata]	[more]

BLAST of Carg25215 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7XU38	6.4e-166	64.04	Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV...	[more]
K7NBR2	3.1e-104	41.34	Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=...	[more]
Q8L7D5	1.0e-99	42.73	Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3	[more]
O64989	1.9e-77	36.91	Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2	[more]
Q5CCK3	6.2e-76	37.12	Cytochrome P450 90B2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90B2 PE=1 SV...	[more]

BLAST of Carg25215 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E749	8.0e-252	99.77	cytochrome P450 87A3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114312...	[more]
A0A6J1KZQ5	4.4e-250	98.84	cytochrome P450 87A3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500308...	[more]
A0A6J1E6G8	1.5e-245	99.76	cytochrome P450 87A3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1114312...	[more]
A0A6J1L8P6	8.0e-244	98.80	cytochrome P450 87A3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111500308...	[more]
A0A6J1EA00	3.1e-240	99.76	cytochrome P450 87A3-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC1114312...	[more]

BLAST of Carg25215 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12740.1	5.9e-199	75.87	cytochrome P450, family 87, subfamily A, polypeptide 2	[more]
AT1G12740.2	1.5e-197	75.29	cytochrome P450, family 87, subfamily A, polypeptide 2	[more]
AT1G78490.1	1.9e-104	44.19	cytochrome P450, family 708, subfamily A, polypeptide 3	[more]
AT5G48000.2	7.4e-101	42.73	cytochrome P450, family 708, subfamily A, polypeptide 2	[more]
AT5G48000.3	7.4e-101	42.73	cytochrome P450, family 708, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 410..419 score: 49.71 coord: 419..430 score: 23.5 coord: 279..296 score: 30.72 coord: 336..347 score: 34.31
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 31..429 e-value: 5.7E-59 score: 200.1
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 419..430 score: 9.75 coord: 288..314 score: 21.61 coord: 268..285 score: 28.08 coord: 335..353 score: 27.27 coord: 409..419 score: 49.14 coord: 63..82 score: 28.8 coord: 375..399 score: 22.96
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 22..430 e-value: 1.8E-102 score: 345.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 27..429
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 2..424 e-value: 2.1E-20 score: 70.9
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 8..430
None	No IPR available	PANTHER	PTHR24286:SF11	CYTOCHROME P450, FAMILY 87, SUBFAMILY A, POLYPEPTIDE 2	coord: 8..430
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 412..421

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25215-RA	Carg25215-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen