Carg23842 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg23842
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionUbiquitinyl hydrolase 1
LocationCarg_Chr02: 4249967 .. 4250920 (-)
RNA-Seq ExpressionCarg23842
SyntenyCarg23842
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAGGTACTCCGTCGGTGAAATGAAATTCCGAGCACTTCCATCTCCGACGATTCTCGAAGGCCTGCATTCGTCGTCAACGCAGCCAATGCTCCAGTTCTAATTCTTAACCTAGATTCAGAGGTCTGCGTGCTTCTGAAATTGGAATTTACGCGAGTTCAGGCTGAGGATTTGAGATCTAAGTTGTGATGACGAGCTAGGATTGTCGAGTTTATCACGAGAGGCAAAAACTACAGTTCTGCCTCTTGCACGCGCTCAACAACCTTTTTCAGGTACCTTCACTATCGAATGTTTGACTGTGGAAAAGTTGCTGAACGCTCTCTGATTGGTTGCGATGAAAAAAATCGTTCAATCTTGGAATTTATGATTTGCAATGTAATTAAGATGCAGATGAACGGAATTATACTGTTTTTTTCGTCATTAAGAAAGGATTATGGTGCATAGAGTCTCAGAATTCGAACTTATCGTCTCACTATTTCACGATTTGTTGCTTTCCTTTTGTAATTCAATGTCTTCTTACTGCTAAATCGTTAATCAACATTTAAACGCCTCTTAAAACAGGAAAATGATGCATTTACTCGAGCCAACTTAAATGCAACAGCTGAAAAACTGGTGCTCGAGGAGCCAAACAAAGAACCTTGGACACCATTATCATTTGTCTTTAAGTCTCATCACAACACAGTAACTGGAAATTATGACATAACCATCGAAATAGAGCTTCTTCAATCGATTTGGATGGGCCTGAGGCTACTCATAAGGTTATGGTTTGATGGCATTTGGAATAGTAGGCACTGGGATACACCGGGAAAGATAAACGAATTGTGGTATAATTTGGACAGTGATCTGCTTGATCCTCATCCGTTTAAGGATATTGATGAAGTAAGACAATTTTTGGATCATGTGATTGGTGACAATGGGGAAATTTTGTTTGTTATAAATGACAGGAACTGA

mRNA sequence

ATGGCGAAGGAAAATGATGCATTTACTCGAGCCAACTTAAATGCAACAGCTGAAAAACTGGTGCTCGAGGAGCCAAACAAAGAACCTTGGACACCATTATCATTTGTCTTTAAGTCTCATCACAACACAGTAACTGGAAATTATGACATAACCATCGAAATAGAGCTTCTTCAATCGATTTGGATGGGCCTGAGGCTACTCATAAGGTTATGGTTTGATGGCATTTGGAATAGTAGGCACTGGGATACACCGGGAAAGATAAACGAATTGTGGTATAATTTGGACAGTGATCTGCTTGATCCTCATCCGTTTAAGGATATTGATGAAGTAAGACAATTTTTGGATCATGTGATTGGTGACAATGGGGAAATTTTGTTTGTTATAAATGACAGGAACTGA

Coding sequence (CDS)

ATGGCGAAGGAAAATGATGCATTTACTCGAGCCAACTTAAATGCAACAGCTGAAAAACTGGTGCTCGAGGAGCCAAACAAAGAACCTTGGACACCATTATCATTTGTCTTTAAGTCTCATCACAACACAGTAACTGGAAATTATGACATAACCATCGAAATAGAGCTTCTTCAATCGATTTGGATGGGCCTGAGGCTACTCATAAGGTTATGGTTTGATGGCATTTGGAATAGTAGGCACTGGGATACACCGGGAAAGATAAACGAATTGTGGTATAATTTGGACAGTGATCTGCTTGATCCTCATCCGTTTAAGGATATTGATGAAGTAAGACAATTTTTGGATCATGTGATTGGTGACAATGGGGAAATTTTGTTTGTTATAAATGACAGGAACTGA

Protein sequence

MAKENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQSIWMGLRLLIRLWFDGIWNSRHWDTPGKINELWYNLDSDLLDPHPFKDIDEVRQFLDHVIGDNGEILFVINDRN
Homology
BLAST of Carg23842 vs. NCBI nr
Match: KAG7035373.1 (Josephin-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 282.7 bits (722), Expect = 1.6e-72
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 0

Query: 1   MAKENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQSI 60
           MAKENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQSI
Sbjct: 1   MAKENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQSI 60

Query: 61  WMGLRLLIRLWFDGIWNSRHWDTPGKINELWYNLDSDLLDPHPFKDIDEVRQFLDHVIGD 120
           WMGLRLLIRLWFDGIWNSRHWDTPGKINELWYNLDSDLLDPHPFKDIDEVRQFLDHVIGD
Sbjct: 61  WMGLRLLIRLWFDGIWNSRHWDTPGKINELWYNLDSDLLDPHPFKDIDEVRQFLDHVIGD 120

Query: 121 NGEILFVINDRN 133
           NGEILFVINDRN
Sbjct: 121 NGEILFVINDRN 132

BLAST of Carg23842 vs. NCBI nr
Match: XP_022947023.1 (josephin-like protein [Cucurbita moschata] >XP_022947024.1 josephin-like protein [Cucurbita moschata])

HSP 1 Score: 270.4 bits (690), Expect = 8.2e-69
Identity = 125/130 (96.15%), Postives = 128/130 (98.46%), Query Frame = 0

Query: 3   KENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQSIWM 62
           +ENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFK HHNTVTGNYDITIEIELLQSIWM
Sbjct: 28  QENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKPHHNTVTGNYDITIEIELLQSIWM 87

Query: 63  GLRLLIRLWFDGIWNSRHWDTPGKINELWYNLDSDLLDPHPFKDIDEVRQFLDHVIGDNG 122
           GLRLL++LWFDGIWNSRHWDTPGKINELWYNLDSDLL PHPFKDIDEVRQFLDHVIGDNG
Sbjct: 88  GLRLLMKLWFDGIWNSRHWDTPGKINELWYNLDSDLLAPHPFKDIDEVRQFLDHVIGDNG 147

Query: 123 EILFVINDRN 133
           EILFVINDRN
Sbjct: 148 EILFVINDRN 157

BLAST of Carg23842 vs. NCBI nr
Match: XP_023532315.1 (josephin-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 254.2 bits (648), Expect = 6.1e-64
Identity = 120/130 (92.31%), Postives = 123/130 (94.62%), Query Frame = 0

Query: 3   KENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQSIWM 62
           +ENDAFTRANLNATAEKLV EEPNKEPWTPLSFVFK +H TVTGNYDITIEIELLQSIWM
Sbjct: 28  QENDAFTRANLNATAEKLVFEEPNKEPWTPLSFVFKPYHTTVTGNYDITIEIELLQSIWM 87

Query: 63  GLRLLIRLWFDGIWNSRHWDTPGKINELWYNLDSDLLDPHPFKDIDEVRQFLDHVIGDNG 122
           GLRLLI LWFDGIWNSRHWD PGKINELWYNLDSDLL  HPFKDIDEVRQFLDHVIGDNG
Sbjct: 88  GLRLLINLWFDGIWNSRHWDAPGKINELWYNLDSDLL-AHPFKDIDEVRQFLDHVIGDNG 147

Query: 123 EILFVINDRN 133
           EILFVINDR+
Sbjct: 148 EILFVINDRS 156

BLAST of Carg23842 vs. NCBI nr
Match: KAG6605424.1 (Josephin-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 251.9 bits (642), Expect = 3.0e-63
Identity = 116/117 (99.15%), Postives = 117/117 (100.00%), Query Frame = 0

Query: 3   KENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQSIWM 62
           +ENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQSIWM
Sbjct: 53  QENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQSIWM 112

Query: 63  GLRLLIRLWFDGIWNSRHWDTPGKINELWYNLDSDLLDPHPFKDIDEVRQFLDHVIG 120
           GLRLLIRLWFDGIWNSRHWDTPGKINELWYNLDSDLLDPHPFKDIDEVRQFLDHVIG
Sbjct: 113 GLRLLIRLWFDGIWNSRHWDTPGKINELWYNLDSDLLDPHPFKDIDEVRQFLDHVIG 169

BLAST of Carg23842 vs. NCBI nr
Match: XP_038902688.1 (josephin-like protein [Benincasa hispida] >XP_038902689.1 josephin-like protein [Benincasa hispida])

HSP 1 Score: 180.3 bits (456), Expect = 1.1e-41
Identity = 94/157 (59.87%), Postives = 106/157 (67.52%), Query Frame = 0

Query: 3   KENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQS--- 62
           +E DAFTRANLNA AEKLVL+EPN+E WTPLSFVFK HHNTVTGNYDI + I  L+    
Sbjct: 28  QEKDAFTRANLNAIAEKLVLDEPNEERWTPLSFVFKPHHNTVTGNYDINVLISALEEKGK 87

Query: 63  --IW---------------------MGLRLLIRL-WFDGIWNSRHWDTPGKINELWYNLD 122
             +W                     MG+ L + +  F GIW SRHW    KIN LWYNLD
Sbjct: 88  IVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLD 147

Query: 123 SDLLDPHPFKDIDEVRQFLDHVIGDNGEILFVINDRN 133
           SDL  PHPFKD DEVRQFLDHVIG+NGEIL ++NDRN
Sbjct: 148 SDLPAPHPFKDTDEVRQFLDHVIGNNGEILLIMNDRN 184

BLAST of Carg23842 vs. ExPASy Swiss-Prot
Match: O82391 (Josephin-like protein OS=Arabidopsis thaliana OX=3702 GN=At2g29640 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 9.6e-28
Identity = 64/137 (46.72%), Postives = 83/137 (60.58%), Query Frame = 0

Query: 3   KENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQS--- 62
           ++ DAFT+ +LN+ AEKL   +PNKE WTPLSFV K HHNT+TGNYD+ + I  L+    
Sbjct: 28  QDKDAFTKESLNSIAEKLETNDPNKETWTPLSFVLKPHHNTITGNYDVNVMITALEGKGK 87

Query: 63  --IW------------------MGLRLLIRL-WFDGIWNSRHWDTPGKINELWYNLDSDL 116
             +W                  MG+ L + +  + G+W SRHW    KIN +WYNLDSDL
Sbjct: 88  SVVWHDKRIGASSIDLDDADTLMGIVLNVPVKRYGGLWRSRHWVVVRKINGVWYNLDSDL 147

BLAST of Carg23842 vs. ExPASy TrEMBL
Match: A0A6J1G5H0 (Ubiquitinyl hydrolase 1 OS=Cucurbita moschata OX=3662 GN=LOC111451025 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 4.0e-69
Identity = 125/130 (96.15%), Postives = 128/130 (98.46%), Query Frame = 0

Query: 3   KENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQSIWM 62
           +ENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFK HHNTVTGNYDITIEIELLQSIWM
Sbjct: 28  QENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKPHHNTVTGNYDITIEIELLQSIWM 87

Query: 63  GLRLLIRLWFDGIWNSRHWDTPGKINELWYNLDSDLLDPHPFKDIDEVRQFLDHVIGDNG 122
           GLRLL++LWFDGIWNSRHWDTPGKINELWYNLDSDLL PHPFKDIDEVRQFLDHVIGDNG
Sbjct: 88  GLRLLMKLWFDGIWNSRHWDTPGKINELWYNLDSDLLAPHPFKDIDEVRQFLDHVIGDNG 147

Query: 123 EILFVINDRN 133
           EILFVINDRN
Sbjct: 148 EILFVINDRN 157

BLAST of Carg23842 vs. ExPASy TrEMBL
Match: A0A6J1JI12 (Ubiquitinyl hydrolase 1 OS=Cucurbita maxima OX=3661 GN=LOC111484533 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 1.2e-41
Identity = 94/157 (59.87%), Postives = 107/157 (68.15%), Query Frame = 0

Query: 3   KENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQS--- 62
           +EN+AFTRA+LNA AEKLVL+EPNKEPWTPLSFVFK HHN+VTGNYDI + I  L+    
Sbjct: 28  QENNAFTRAHLNAIAEKLVLDEPNKEPWTPLSFVFKPHHNSVTGNYDINVLISALEEKGK 87

Query: 63  --IW---------------------MGLRLLIRL-WFDGIWNSRHWDTPGKINELWYNLD 122
             +W                     MG+ L + +  F GIW SRHW    KINELWYNLD
Sbjct: 88  SVVWHDHRNGASSIDLDGPEATHKLMGIVLNVPVRRFGGIWKSRHWVALRKINELWYNLD 147

Query: 123 SDLLDPHPFKDIDEVRQFLDHVIGDNGEILFVINDRN 133
           SDL  PH FKD DEVRQFLD+VI DNGEIL V+NDRN
Sbjct: 148 SDLPTPHTFKDTDEVRQFLDNVINDNGEILLVMNDRN 184

BLAST of Carg23842 vs. ExPASy TrEMBL
Match: A0A6J1FYW5 (Ubiquitinyl hydrolase 1 OS=Cucurbita moschata OX=3662 GN=LOC111449101 PE=4 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.3e-40
Identity = 91/157 (57.96%), Postives = 106/157 (67.52%), Query Frame = 0

Query: 3   KENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQS--- 62
           +ENDAFTRA+LNA +EKLVL+EPN+EPWTPLSFVFK HHN+VTGNYDI + I  ++    
Sbjct: 28  QENDAFTRAHLNAISEKLVLDEPNREPWTPLSFVFKPHHNSVTGNYDINVLISAMEEKGK 87

Query: 63  --IW---------------------MGLRLLIRL-WFDGIWNSRHWDTPGKINELWYNLD 122
             +W                     MG+ L + +  F GIW SRHW    KINE WYNLD
Sbjct: 88  SVVWHDHRNGASSIDLDGPEATHKLMGIVLNVPVRRFGGIWKSRHWVALRKINESWYNLD 147

Query: 123 SDLLDPHPFKDIDEVRQFLDHVIGDNGEILFVINDRN 133
           SDL  PH FKD DEVRQFLD+VI DNGEIL V+NDRN
Sbjct: 148 SDLHTPHTFKDTDEVRQFLDNVINDNGEILLVMNDRN 184

BLAST of Carg23842 vs. ExPASy TrEMBL
Match: A0A0A0KBN9 (Ubiquitinyl hydrolase 1 OS=Cucumis sativus OX=3659 GN=Csa_6G187930 PE=4 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 6.6e-40
Identity = 91/157 (57.96%), Postives = 103/157 (65.61%), Query Frame = 0

Query: 3   KENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQS--- 62
           +E DAFTRANLNA AEKLV + PN+E WTPLS VFK HHNTVTGNYDI + I  L+    
Sbjct: 28  QEKDAFTRANLNAIAEKLVRDVPNQETWTPLSIVFKPHHNTVTGNYDINVLISALEEKGK 87

Query: 63  --IW---------------------MGLRLLIRL-WFDGIWNSRHWDTPGKINELWYNLD 122
             +W                     MG+ L + +  F GIW SRHW    KI+ LWYNLD
Sbjct: 88  SVVWHDHRNGAFSIDLDGPDHTCKLMGIVLNVSVRRFGGIWKSRHWVALRKIDGLWYNLD 147

Query: 123 SDLLDPHPFKDIDEVRQFLDHVIGDNGEILFVINDRN 133
           SDL DPHPFKD DE+RQFLDHVI DNGEIL V+NDRN
Sbjct: 148 SDLPDPHPFKDTDELRQFLDHVINDNGEILLVMNDRN 184

BLAST of Carg23842 vs. ExPASy TrEMBL
Match: A0A1S4E4I3 (Ubiquitinyl hydrolase 1 OS=Cucumis melo OX=3656 GN=LOC103502098 PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 2.5e-39
Identity = 90/157 (57.32%), Postives = 103/157 (65.61%), Query Frame = 0

Query: 3   KENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQS--- 62
           +E DAFTRANLNA AEKLV +EPN+E WTPLS VFK HHNTVTGNYDI + I  L+    
Sbjct: 29  QEKDAFTRANLNAIAEKLVRDEPNQETWTPLSIVFKPHHNTVTGNYDINVLISALEEKGK 88

Query: 63  --IW---------------------MGLRLLIRL-WFDGIWNSRHWDTPGKINELWYNLD 122
             +W                     MG+ L + +  F GIW SRHW    KI+ LWYNLD
Sbjct: 89  SVVWHDHRNGASTIDLDGPDDTRKLMGIVLNVSVRRFGGIWKSRHWVALRKIDGLWYNLD 148

Query: 123 SDLLDPHPFKDIDEVRQFLDHVIGDNGEILFVINDRN 133
           SDL  PHPFKD +EVR+FLDHVI DNGEIL V+NDRN
Sbjct: 149 SDLPAPHPFKDTEEVREFLDHVINDNGEILLVMNDRN 185

BLAST of Carg23842 vs. TAIR 10
Match: AT2G29640.1 (JOSEPHIN-like protein )

HSP 1 Score: 124.4 bits (311), Expect = 6.8e-29
Identity = 64/137 (46.72%), Postives = 83/137 (60.58%), Query Frame = 0

Query: 3   KENDAFTRANLNATAEKLVLEEPNKEPWTPLSFVFKSHHNTVTGNYDITIEIELLQS--- 62
           ++ DAFT+ +LN+ AEKL   +PNKE WTPLSFV K HHNT+TGNYD+ + I  L+    
Sbjct: 28  QDKDAFTKESLNSIAEKLETNDPNKETWTPLSFVLKPHHNTITGNYDVNVMITALEGKGK 87

Query: 63  --IW------------------MGLRLLIRL-WFDGIWNSRHWDTPGKINELWYNLDSDL 116
             +W                  MG+ L + +  + G+W SRHW    KIN +WYNLDSDL
Sbjct: 88  SVVWHDKRIGASSIDLDDADTLMGIVLNVPVKRYGGLWRSRHWVVVRKINGVWYNLDSDL 147

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035373.11.6e-72100.00Josephin-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022947023.18.2e-6996.15josephin-like protein [Cucurbita moschata] >XP_022947024.1 josephin-like protein... [more]
XP_023532315.16.1e-6492.31josephin-like protein [Cucurbita pepo subsp. pepo][more]
KAG6605424.13.0e-6399.15Josephin-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038902688.11.1e-4159.87josephin-like protein [Benincasa hispida] >XP_038902689.1 josephin-like protein ... [more]
Match NameE-valueIdentityDescription
O823919.6e-2846.72Josephin-like protein OS=Arabidopsis thaliana OX=3702 GN=At2g29640 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G5H04.0e-6996.15Ubiquitinyl hydrolase 1 OS=Cucurbita moschata OX=3662 GN=LOC111451025 PE=4 SV=1[more]
A0A6J1JI121.2e-4159.87Ubiquitinyl hydrolase 1 OS=Cucurbita maxima OX=3661 GN=LOC111484533 PE=4 SV=1[more]
A0A6J1FYW51.3e-4057.96Ubiquitinyl hydrolase 1 OS=Cucurbita moschata OX=3662 GN=LOC111449101 PE=4 SV=1[more]
A0A0A0KBN96.6e-4057.96Ubiquitinyl hydrolase 1 OS=Cucumis sativus OX=3659 GN=Csa_6G187930 PE=4 SV=1[more]
A0A1S4E4I32.5e-3957.32Ubiquitinyl hydrolase 1 OS=Cucumis melo OX=3656 GN=LOC103502098 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G29640.16.8e-2946.72JOSEPHIN-like protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006155Josephin domainSMARTSM01246Josephin_2coord: 4..130
e-value: 5.9E-5
score: 8.2
IPR006155Josephin domainPFAMPF02099Josephincoord: 78..127
e-value: 7.1E-8
score: 32.6
IPR006155Josephin domainPROSITEPS50957JOSEPHINcoord: 1..132
score: 10.394816
NoneNo IPR availableGENE3D3.90.70.40coord: 69..131
e-value: 1.4E-8
score: 36.3
NoneNo IPR availablePANTHERPTHR13291:SF0JOSEPHIN-LIKE PROTEINcoord: 4..59
coord: 74..131
IPR040053Josephin-1/2PANTHERPTHR13291JOSEPHIN 1, 2coord: 4..59
coord: 74..131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23842-RACarg23842-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016579 protein deubiquitination
molecular_function GO:0004843 thiol-dependent deubiquitinase