Carg23133 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg23133
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRNA helicase
LocationCarg_Chr08: 3273008 .. 3275995 (+)
RNA-Seq ExpressionCarg23133
SyntenyCarg23133
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATAGCTCCACCGGCTGATAGACGGTTTTGCAGTCAGAATTTCAGAAGACTCGGTTGGTTGTTTCCTTCCGCCCACCGCTGCCGTTTTTCTCGTCGGCGACCCTCCACCGACCAATCTCCCTCTGTATGTCAGTACTTTTTGCTAGTCTCGATCATCCATATCACTTTCTCCATTTGGTTCTTATTCGAATCCTGACAACGAAGAAGTAGAGGAGTGATCTACCTCCCGTGGAACAGTTTCGAGTAAATTGTAGTTTTTTTTTTGCCTTTTGATAATGTTTGTGGGTTCTTTATGTTGAATCACTTGTCGACTAAGTTCTTCGGTTCATATATTTCACTCTAAATCTGTCTCTGGGTGGTTCGAACTGGAATGGGATTGTATTTCGAACCCTAATCGGGAGAATCTAATTCGAGAGTCTTGTTATTGTTGTTATCTGCATTTGAGGTTTTTTTGTTCGGTTAATTCTAATTATTATCTGCGTGTAATATGAAATCTAATAGTTTCTAGGGTTAGCTGCGAAGTGTCTAGGGATTGGATTTATGATTGTCTTTTGATTATTTTTGGGCGCAGAGAGGGGATTCTATGATGGATGTAGTAATGAAGGACGAAAAAAGCATGGAAGAAGAAGAGGATTATGTTGAATATGTTCCCGTGGCAAAGCGTAGAGCAATGGAAGCTCAAAAGATTCTTATGCGCAAGGGCAAGGCTTCTGCTCTTGAGGAAGAGCTGGAGAAATCAAGACTTGCTGAAGCTAAACCTAGTCTCCTTGTAAAAGCGTCTCAGATGAAGCGTGACCAGCCAGAAGTTAGTCCAACTGAGCAGATTGTTCAGCAGGAGAAGGAGATGATTGAGCACTTATCTGATCGTAAGACTCTAATGTCGGTTCGAGAATTAGCAAAAGGAATTACTTACACAGAGCCATTATTAACTGGATGGAAGCCACCATTGCCTATCAGGAGGATGCCGAGAAAGGCGTGTGATTTGATTCGCAAACAATGGCACATTATAGTTGATGGTGACGATATTCCACCGCCAATTAAAAATTTCAAGGACATGAGACTCCCAGAACCAGTTTTGAAGAAGTTGAAAGAAAAGGGAATTGTGCAGCCTACTCCCATTCAGGTCCAAGGTTTACCGGTAATTTTATCTGGTCGAGATATGATTGGGATTGCATTTACGGGTTCGGGAAAGACCCTAGTTTTTGTTCTTCCTTTGATAATGATTGCACTACAAGAAGAGGTCATGATGCCAATTGTTTCTGGAGAAGGGCCTTTTGGTTTGATTATTTGTCCTTCAAGAGAGCTTGCTAGACAGACCTACGAAGTGGTGGAACAATTTTTAATTCCCATGAAGGAGGCTGGATATCCTGAACTAAGGCCTTTACTCTGTATAGGTGGAGTGGATATGAGATCACAAATAGAGATTGTGAAGAAGGGTGTTCACATTGTTGTTGCAACCCCTGGGAGGTTGAAGGACATGTTGGCAAAAAAGAAAATGAACCTTGACAATTGCAGGTAACATATTTCGTCAGTTTCTATTACATTATTTGGGTTAGACTCTCTGAAAACGAATTTGAAATGTTCTAGCCAACCTGTGATAGCGAGTAGCAACATAACTTATCGATAATATTTCTCTCGTGTGGAAAACCGTCAATTGTTTGGCAAAGAAATCCATGCTTGCTTCCATCTTCACTATAAACCTTTTTTGTATGGTTATTTTTGTAGATGATTTTGTTGTTACACATCAACTTGTTTCCCATGACTTATTTTAGCTGATGCAAACTGATGATTACTTTACGTTCTCAATTTAGTTTTCCTCTTGGTTGAAAATAATGATACAAATGCTGCTATTGTCATAGGTATCTTACCTTAGATGAGGCCGATAGATTGGTGGATCTGGGATTTGAGGACGATATAAGGGAAGTTTTCGATCATTTCAAGGCGCAACGCCAAACTCTCTTATTTTCTGCCACAATGCCTACCAAAATTCAAAATTTTGCAAGAAGTGCCCTGGTAAAGCCTGTAACTGTTAACGTGGGAAGAGCTGGAGCTGCAAATCTTGATGTTATCCAGGAGGTCGAGTACGTGAAACAAGAGGCGAAAATTGTTTACTTGCTCGAGTGCCTGCAGAAAACTCCACCTCCAGTTTTGATATTTTGTGAGAATAAAGCCGACGTCGATGACATTCACGAGTACCTTCTCTTGAAAGGTGTTGAAGCAGTGGCAATTCATGGAGGAAAAGATCAGGAAGAGAGAGAGTATGCTATATCATCCTTTAAGGCAAGCAAGAAAGATGTATTAGTTGCTACTGATGTTGCATCAAAGGGTTTAGATTTTCCAGACATTCAGCACGTTATCAATTACGATATGCCAGCTGAAATCGAGAACTATGTCCATAGGATAGGACGAACTGGACGGTGTGGTAAGACAGGAATTGCAACAACTTTCATAAACAAGAATCAAAGCGAAACAACTCTCCTTGATTTGAAGCACCTTCTGCAAGAAGCAAAACAGAGGATTCCACCAGTGTTGGCTGAGCTAAACGATCCAATGGAGGATGTCGAAGCGATCACCAATGCCAGCGGAGTCAAGGGCTGCGCTTATTGTGGCGGTCTTGGTCACCGTATCCGTGACTGCCCCAAGTTAGAACACCAGAAAAGCATGGCAATTGCAAGTTCTAGAAGAGATTATTTTGGATCAGGTGGTTACAGAGGAGAAATTTGAGAAAGCTAAGAACAGAACGGATATTTTTGTTTTGTTTCCCATTTACTCTCCACTGATTGTACCTGTACAAACTGATAAGTTATTATGGTTAAAACGTTCCAATGAAATCGTCTAGTTTACTTGGTGGATGTCAATACGTTCTTTTGTCTGCGTTGATTTTATCTCAGTGACAATGACAATCAGTGTTATTTATCTTTGCAACAGGACATCATTAATTGACATTATTGACACTGTTTCTTACAAATATAGATAAGGATTGTCTGT

mRNA sequence

AATAGCTCCACCGGCTGATAGACGGTTTTGCAGTCAGAATTTCAGAAGACTCGGTTGGTTGTTTCCTTCCGCCCACCGCTGCCGTTTTTCTCGTCGGCGACCCTCCACCGACCAATCTCCCTCTAGAGGGGATTCTATGATGGATGTAGTAATGAAGGACGAAAAAAGCATGGAAGAAGAAGAGGATTATGTTGAATATGTTCCCGTGGCAAAGCGTAGAGCAATGGAAGCTCAAAAGATTCTTATGCGCAAGGGCAAGGCTTCTGCTCTTGAGGAAGAGCTGGAGAAATCAAGACTTGCTGAAGCTAAACCTAGTCTCCTTGTAAAAGCGTCTCAGATGAAGCGTGACCAGCCAGAAGTTAGTCCAACTGAGCAGATTGTTCAGCAGGAGAAGGAGATGATTGAGCACTTATCTGATCGTAAGACTCTAATGTCGGTTCGAGAATTAGCAAAAGGAATTACTTACACAGAGCCATTATTAACTGGATGGAAGCCACCATTGCCTATCAGGAGGATGCCGAGAAAGGCGTGTGATTTGATTCGCAAACAATGGCACATTATAGTTGATGGTGACGATATTCCACCGCCAATTAAAAATTTCAAGGACATGAGACTCCCAGAACCAGTTTTGAAGAAGTTGAAAGAAAAGGGAATTGTGCAGCCTACTCCCATTCAGGTCCAAGGTTTACCGGTAATTTTATCTGGTCGAGATATGATTGGGATTGCATTTACGGGTTCGGGAAAGACCCTAGTTTTTGTTCTTCCTTTGATAATGATTGCACTACAAGAAGAGGTCATGATGCCAATTGTTTCTGGAGAAGGGCCTTTTGGTTTGATTATTTGTCCTTCAAGAGAGCTTGCTAGACAGACCTACGAAGTGGTGGAACAATTTTTAATTCCCATGAAGGAGGCTGGATATCCTGAACTAAGGCCTTTACTCTGTATAGGTGGAGTGGATATGAGATCACAAATAGAGATTGTGAAGAAGGGTGTTCACATTGTTGTTGCAACCCCTGGGAGGTTGAAGGACATGTTGGCAAAAAAGAAAATGAACCTTGACAATTGCAGGTATCTTACCTTAGATGAGGCCGATAGATTGGTGGATCTGGGATTTGAGGACGATATAAGGGAAGTTTTCGATCATTTCAAGGCGCAACGCCAAACTCTCTTATTTTCTGCCACAATGCCTACCAAAATTCAAAATTTTGCAAGAAGTGCCCTGGTAAAGCCTGTAACTGTTAACGTGGGAAGAGCTGGAGCTGCAAATCTTGATGTTATCCAGGAGGTCGAGTACGTGAAACAAGAGGCGAAAATTGTTTACTTGCTCGAGTGCCTGCAGAAAACTCCACCTCCAGTTTTGATATTTTGTGAGAATAAAGCCGACGTCGATGACATTCACGAGTACCTTCTCTTGAAAGGTGTTGAAGCAGTGGCAATTCATGGAGGAAAAGATCAGGAAGAGAGAGAGTATGCTATATCATCCTTTAAGGCAAGCAAGAAAGATGTATTAGTTGCTACTGATGTTGCATCAAAGGGTTTAGATTTTCCAGACATTCAGCACGTTATCAATTACGATATGCCAGCTGAAATCGAGAACTATGTCCATAGGATAGGACGAACTGGACGGTGTGGTAAGACAGGAATTGCAACAACTTTCATAAACAAGAATCAAAGCGAAACAACTCTCCTTGATTTGAAGCACCTTCTGCAAGAAGCAAAACAGAGGATTCCACCAGTGTTGGCTGAGCTAAACGATCCAATGGAGGATGTCGAAGCGATCACCAATGCCAGCGGAGTCAAGGGCTGCGCTTATTGTGGCGGTCTTGGTCACCGTATCCGTGACTGCCCCAAGTTAGAACACCAGAAAAGCATGGCAATTGCAAGTTCTAGAAGAGATTATTTTGGATCAGGTGGTTACAGAGGAGAAATTTGAGAAAGCTAAGAACAGAACGGATATTTTTGTTTTGTTTCCCATTTACTCTCCACTGATTGTACCTGTACAAACTGATAAGTTATTATGGTTAAAACGTTCCAATGAAATCGTCTAGTTTACTTGGTGGATGTCAATACGTTCTTTTGTCTGCGTTGATTTTATCTCAGTGACAATGACAATCAGTGTTATTTATCTTTGCAACAGGACATCATTAATTGACATTATTGACACTGTTTCTTACAAATATAGATAAGGATTGTCTGT

Coding sequence (CDS)

ATGATGGATGTAGTAATGAAGGACGAAAAAAGCATGGAAGAAGAAGAGGATTATGTTGAATATGTTCCCGTGGCAAAGCGTAGAGCAATGGAAGCTCAAAAGATTCTTATGCGCAAGGGCAAGGCTTCTGCTCTTGAGGAAGAGCTGGAGAAATCAAGACTTGCTGAAGCTAAACCTAGTCTCCTTGTAAAAGCGTCTCAGATGAAGCGTGACCAGCCAGAAGTTAGTCCAACTGAGCAGATTGTTCAGCAGGAGAAGGAGATGATTGAGCACTTATCTGATCGTAAGACTCTAATGTCGGTTCGAGAATTAGCAAAAGGAATTACTTACACAGAGCCATTATTAACTGGATGGAAGCCACCATTGCCTATCAGGAGGATGCCGAGAAAGGCGTGTGATTTGATTCGCAAACAATGGCACATTATAGTTGATGGTGACGATATTCCACCGCCAATTAAAAATTTCAAGGACATGAGACTCCCAGAACCAGTTTTGAAGAAGTTGAAAGAAAAGGGAATTGTGCAGCCTACTCCCATTCAGGTCCAAGGTTTACCGGTAATTTTATCTGGTCGAGATATGATTGGGATTGCATTTACGGGTTCGGGAAAGACCCTAGTTTTTGTTCTTCCTTTGATAATGATTGCACTACAAGAAGAGGTCATGATGCCAATTGTTTCTGGAGAAGGGCCTTTTGGTTTGATTATTTGTCCTTCAAGAGAGCTTGCTAGACAGACCTACGAAGTGGTGGAACAATTTTTAATTCCCATGAAGGAGGCTGGATATCCTGAACTAAGGCCTTTACTCTGTATAGGTGGAGTGGATATGAGATCACAAATAGAGATTGTGAAGAAGGGTGTTCACATTGTTGTTGCAACCCCTGGGAGGTTGAAGGACATGTTGGCAAAAAAGAAAATGAACCTTGACAATTGCAGGTATCTTACCTTAGATGAGGCCGATAGATTGGTGGATCTGGGATTTGAGGACGATATAAGGGAAGTTTTCGATCATTTCAAGGCGCAACGCCAAACTCTCTTATTTTCTGCCACAATGCCTACCAAAATTCAAAATTTTGCAAGAAGTGCCCTGGTAAAGCCTGTAACTGTTAACGTGGGAAGAGCTGGAGCTGCAAATCTTGATGTTATCCAGGAGGTCGAGTACGTGAAACAAGAGGCGAAAATTGTTTACTTGCTCGAGTGCCTGCAGAAAACTCCACCTCCAGTTTTGATATTTTGTGAGAATAAAGCCGACGTCGATGACATTCACGAGTACCTTCTCTTGAAAGGTGTTGAAGCAGTGGCAATTCATGGAGGAAAAGATCAGGAAGAGAGAGAGTATGCTATATCATCCTTTAAGGCAAGCAAGAAAGATGTATTAGTTGCTACTGATGTTGCATCAAAGGGTTTAGATTTTCCAGACATTCAGCACGTTATCAATTACGATATGCCAGCTGAAATCGAGAACTATGTCCATAGGATAGGACGAACTGGACGGTGTGGTAAGACAGGAATTGCAACAACTTTCATAAACAAGAATCAAAGCGAAACAACTCTCCTTGATTTGAAGCACCTTCTGCAAGAAGCAAAACAGAGGATTCCACCAGTGTTGGCTGAGCTAAACGATCCAATGGAGGATGTCGAAGCGATCACCAATGCCAGCGGAGTCAAGGGCTGCGCTTATTGTGGCGGTCTTGGTCACCGTATCCGTGACTGCCCCAAGTTAGAACACCAGAAAAGCATGGCAATTGCAAGTTCTAGAAGAGATTATTTTGGATCAGGTGGTTACAGAGGAGAAATTTGA

Protein sequence

MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSLLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPPLPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQVQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLAKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Homology
BLAST of Carg23133 vs. NCBI nr
Match: XP_023004285.1 (DEAD-box ATP-dependent RNA helicase 35 [Cucurbita maxima] >XP_023004286.1 DEAD-box ATP-dependent RNA helicase 35 [Cucurbita maxima] >XP_023514207.1 DEAD-box ATP-dependent RNA helicase 35 [Cucurbita pepo subsp. pepo] >KAG7025731.1 DEAD-box ATP-dependent RNA helicase 35 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1172.1 bits (3031), Expect = 0.0e+00
Identity = 598/598 (100.00%), Postives = 598/598 (100.00%), Query Frame = 0

Query: 1   MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPS 60
           MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPS
Sbjct: 1   MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPS 60

Query: 61  LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP 120
           LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP
Sbjct: 61  LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP 120

Query: 121 PLPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ 180
           PLPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ
Sbjct: 121 PLPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ 180

Query: 181 VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE 240
           VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE
Sbjct: 181 VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE 240

Query: 241 LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML 300
           LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML
Sbjct: 241 LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML 300

Query: 301 AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS 360
           AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS
Sbjct: 301 AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS 360

Query: 361 ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI 420
           ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI
Sbjct: 361 ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI 420

Query: 421 HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD 480
           HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD
Sbjct: 421 HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD 480

Query: 481 MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND 540
           MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND
Sbjct: 481 MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND 540

Query: 541 PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 599
           PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 541 PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598

BLAST of Carg23133 vs. NCBI nr
Match: KAG6593383.1 (DEAD-box ATP-dependent RNA helicase 35, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1172.1 bits (3031), Expect = 0.0e+00
Identity = 598/598 (100.00%), Postives = 598/598 (100.00%), Query Frame = 0

Query: 1   MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPS 60
           MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPS
Sbjct: 73  MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPS 132

Query: 61  LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP 120
           LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP
Sbjct: 133 LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP 192

Query: 121 PLPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ 180
           PLPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ
Sbjct: 193 PLPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ 252

Query: 181 VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE 240
           VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE
Sbjct: 253 VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE 312

Query: 241 LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML 300
           LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML
Sbjct: 313 LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML 372

Query: 301 AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS 360
           AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS
Sbjct: 373 AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS 432

Query: 361 ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI 420
           ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI
Sbjct: 433 ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI 492

Query: 421 HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD 480
           HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD
Sbjct: 493 HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD 552

Query: 481 MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND 540
           MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND
Sbjct: 553 MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND 612

Query: 541 PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 599
           PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 613 PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 670

BLAST of Carg23133 vs. NCBI nr
Match: XP_022960221.1 (DEAD-box ATP-dependent RNA helicase 35-like [Cucurbita moschata] >XP_022960222.1 DEAD-box ATP-dependent RNA helicase 35-like [Cucurbita moschata])

HSP 1 Score: 1170.2 bits (3026), Expect = 0.0e+00
Identity = 597/598 (99.83%), Postives = 598/598 (100.00%), Query Frame = 0

Query: 1   MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPS 60
           MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPS
Sbjct: 1   MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPS 60

Query: 61  LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP 120
           LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP
Sbjct: 61  LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP 120

Query: 121 PLPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ 180
           PLPIRRMPRKACDLIRKQWHIIVDGD+IPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ
Sbjct: 121 PLPIRRMPRKACDLIRKQWHIIVDGDNIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ 180

Query: 181 VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE 240
           VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE
Sbjct: 181 VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE 240

Query: 241 LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML 300
           LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML
Sbjct: 241 LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML 300

Query: 301 AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS 360
           AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS
Sbjct: 301 AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS 360

Query: 361 ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI 420
           ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI
Sbjct: 361 ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI 420

Query: 421 HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD 480
           HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD
Sbjct: 421 HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD 480

Query: 481 MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND 540
           MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND
Sbjct: 481 MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND 540

Query: 541 PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 599
           PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 541 PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598

BLAST of Carg23133 vs. NCBI nr
Match: XP_008461142.1 (PREDICTED: DEAD-box ATP-dependent RNA helicase 35 [Cucumis melo] >XP_008461143.1 PREDICTED: DEAD-box ATP-dependent RNA helicase 35 [Cucumis melo] >KAA0058735.1 DEAD-box ATP-dependent RNA helicase 35 [Cucumis melo var. makuwa])

HSP 1 Score: 1162.9 bits (3007), Expect = 0.0e+00
Identity = 591/597 (98.99%), Postives = 596/597 (99.83%), Query Frame = 0

Query: 2   MDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL 61
           MDV MKDEKS+EEE+DYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL
Sbjct: 1   MDVQMKDEKSLEEEDDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL 60

Query: 62  LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 121
           LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP
Sbjct: 61  LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120

Query: 122 LPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV 181
           LPIRRMP+KACDLIRKQWHIIVDGD+IPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV
Sbjct: 121 LPIRRMPKKACDLIRKQWHIIVDGDEIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV 180

Query: 182 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 241
           QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL
Sbjct: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240

Query: 242 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLA 301
           ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIE+VKKGVHIVVATPGRLKDMLA
Sbjct: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300

Query: 302 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 361
           KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA
Sbjct: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360

Query: 362 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 421
           LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH
Sbjct: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420

Query: 422 EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM 481
           EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM
Sbjct: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM 480

Query: 482 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 541
           PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP
Sbjct: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540

Query: 542 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 599
           MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 597

BLAST of Carg23133 vs. NCBI nr
Match: XP_038885065.1 (DEAD-box ATP-dependent RNA helicase 35 [Benincasa hispida] >XP_038885066.1 DEAD-box ATP-dependent RNA helicase 35 [Benincasa hispida] >XP_038885067.1 DEAD-box ATP-dependent RNA helicase 35 [Benincasa hispida])

HSP 1 Score: 1161.7 bits (3004), Expect = 0.0e+00
Identity = 590/598 (98.66%), Postives = 596/598 (99.67%), Query Frame = 0

Query: 1   MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPS 60
           MMDV MKDEKS+EEE+DYVEYVPVAKRRAMEAQKILM KGKASALEEELEKSRLAEAKPS
Sbjct: 1   MMDVQMKDEKSLEEEDDYVEYVPVAKRRAMEAQKILMHKGKASALEEELEKSRLAEAKPS 60

Query: 61  LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP 120
           LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP
Sbjct: 61  LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP 120

Query: 121 PLPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ 180
           PLPIRRMP+KACDLIRKQWHIIVDGD+IPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ
Sbjct: 121 PLPIRRMPKKACDLIRKQWHIIVDGDEIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ 180

Query: 181 VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE 240
           VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE
Sbjct: 181 VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE 240

Query: 241 LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML 300
           LARQTYEVVEQFL+PMKEAGYPELRPLLCIGGVDMRSQIE+VKKGVHIVVATPGRLKDML
Sbjct: 241 LARQTYEVVEQFLLPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDML 300

Query: 301 AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS 360
           AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS
Sbjct: 301 AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS 360

Query: 361 ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI 420
           ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI
Sbjct: 361 ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI 420

Query: 421 HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD 480
           HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD
Sbjct: 421 HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD 480

Query: 481 MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND 540
           MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND
Sbjct: 481 MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND 540

Query: 541 PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 599
           PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 541 PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598

BLAST of Carg23133 vs. ExPASy Swiss-Prot
Match: Q9LU46 (DEAD-box ATP-dependent RNA helicase 35 OS=Arabidopsis thaliana OX=3702 GN=RH35 PE=2 SV=1)

HSP 1 Score: 1036.6 bits (2679), Expect = 1.1e-301
Identity = 521/590 (88.31%), Postives = 555/590 (94.07%), Query Frame = 0

Query: 9   EKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSLLVKASQM 68
           E  MEE + Y+EYV VA+RRA+ AQKIL RKGKAS LEEE +K +LAEAKPSLLV+A+Q+
Sbjct: 2   ESIMEEADSYIEYVSVAERRAIAAQKILQRKGKASELEEEADKEKLAEAKPSLLVQATQL 61

Query: 69  KRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPPLPIRRMP 128
           KRD PEVS TEQI+ QEKEM+EHLSD+KTLMSVRELAKGITYTEPLLTGWKPPL IR+M 
Sbjct: 62  KRDVPEVSATEQIILQEKEMMEHLSDKKTLMSVRELAKGITYTEPLLTGWKPPLHIRKMS 121

Query: 129 RKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQVQGLPVIL 188
            K  DLIRKQWHIIV+GDDIPPPIKNFKDM+ P PVL  LKEKGIVQPTPIQVQGLPVIL
Sbjct: 122 SKQRDLIRKQWHIIVNGDDIPPPIKNFKDMKFPRPVLDTLKEKGIVQPTPIQVQGLPVIL 181

Query: 189 SGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTYEV 248
           +GRDMIGIAFTGSGKTLVFVLP+IMIALQEE+MMPI +GEGP GLI+CPSRELARQTYEV
Sbjct: 182 AGRDMIGIAFTGSGKTLVFVLPMIMIALQEEMMMPIAAGEGPIGLIVCPSRELARQTYEV 241

Query: 249 VEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLAKKKMNLD 308
           VEQF+ P+ EAGYP LR LLCIGG+DMRSQ+E+VK+GVHIVVATPGRLKDMLAKKKM+LD
Sbjct: 242 VEQFVAPLVEAGYPPLRSLLCIGGIDMRSQLEVVKRGVHIVVATPGRLKDMLAKKKMSLD 301

Query: 309 NCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPVTV 368
            CRYLTLDEADRLVDLGFEDDIREVFDHFK+QRQTLLFSATMPTKIQ FARSALVKPVTV
Sbjct: 302 ACRYLTLDEADRLVDLGFEDDIREVFDHFKSQRQTLLFSATMPTKIQIFARSALVKPVTV 361

Query: 369 NVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLLKG 428
           NVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKT PPVLIFCENKADVDDIHEYLLLKG
Sbjct: 362 NVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTSPPVLIFCENKADVDDIHEYLLLKG 421

Query: 429 VEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENY 488
           VEAVAIHGGKDQE+REYAISSFKA KKDVLVATDVASKGLDFPDIQHVINYDMPAEIENY
Sbjct: 422 VEAVAIHGGKDQEDREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENY 481

Query: 489 VHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVEAI 548
           VHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPME+ E I
Sbjct: 482 VHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEEAETI 541

Query: 549 TNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 599
            NASGVKGCAYCGGLGHRIRDCPKLEHQKS+AI++SR+DYFGSGGYRGEI
Sbjct: 542 ANASGVKGCAYCGGLGHRIRDCPKLEHQKSVAISNSRKDYFGSGGYRGEI 591

BLAST of Carg23133 vs. ExPASy Swiss-Prot
Match: Q0E3X4 (DEAD-box ATP-dependent RNA helicase 35A OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0150100 PE=2 SV=2)

HSP 1 Score: 974.9 bits (2519), Expect = 4.0e-283
Identity = 487/610 (79.84%), Postives = 544/610 (89.18%), Query Frame = 0

Query: 13  EEEEDYVEYVPVAKRRAMEAQKI-LMRKGK-----ASALEEELE---------------- 72
           ++E++Y EY+PVAKRRAMEA ++  +R  K     +SA E   +                
Sbjct: 18  DDEDNYEEYIPVAKRRAMEADRLRRLRLSKPAPPSSSAAEAASDLPPPPPPPPNQPSAGG 77

Query: 73  --KSRLAEAKPSLLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGI 132
                 A AKPSLLVKA+Q+KR  PEV+ TEQ++ QEKEMIEHLSDRKTLMSVRELAKGI
Sbjct: 78  GGGGLEASAKPSLLVKATQLKRAAPEVTHTEQLIMQEKEMIEHLSDRKTLMSVRELAKGI 137

Query: 133 TYTEPLLTGWKPPLPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKL 192
           TY++PL TGWKPPL +RRMPR   D +R++WHI+VDGDD+PPP ++F+D+RLPEP+L+KL
Sbjct: 138 TYSDPLKTGWKPPLRLRRMPRAKADELRRKWHILVDGDDVPPPARDFRDLRLPEPMLRKL 197

Query: 193 KEKGIVQPTPIQVQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGE 252
           +EKGIVQPTPIQVQGLPV+LSGRDMIGIAFTGSGKTLVFVLPLIM+ALQEE+MMPIV GE
Sbjct: 198 REKGIVQPTPIQVQGLPVVLSGRDMIGIAFTGSGKTLVFVLPLIMVALQEEMMMPIVPGE 257

Query: 253 GPFGLIICPSRELARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHI 312
           GPFG+IICPSRELA+QTY+V+EQFL+P+KEAGYPE+RPLLCIGGVDMR+Q+++VKKGVHI
Sbjct: 258 GPFGMIICPSRELAKQTYDVIEQFLVPLKEAGYPEIRPLLCIGGVDMRAQLDVVKKGVHI 317

Query: 313 VVATPGRLKDMLAKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSA 372
           VVATPGRLKD+LAKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSA
Sbjct: 318 VVATPGRLKDLLAKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSA 377

Query: 373 TMPTKIQNFARSALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVL 432
           TMP KIQNFA+SALVKPV VNVGRAGAANLDVIQEVEYVK++A+I+YLLECLQKTPPPVL
Sbjct: 378 TMPKKIQNFAKSALVKPVIVNVGRAGAANLDVIQEVEYVKEDARIIYLLECLQKTPPPVL 437

Query: 433 IFCENKADVDDIHEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGL 492
           +FCENKADVD IHEYLLLKGVEAVAIHGGKDQEERE AI  FK  KKDVLVATDVASKGL
Sbjct: 438 VFCENKADVDYIHEYLLLKGVEAVAIHGGKDQEERENAIEFFKNGKKDVLVATDVASKGL 497

Query: 493 DFPDIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAK 552
           DFPDIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQ+ETTLLDLKHLL+EAK
Sbjct: 498 DFPDIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQTETTLLDLKHLLKEAK 557

Query: 553 QRIPPVLAELNDPMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDY 599
           QRIPPVLAELNDP+ED E +   SGVKGCAYCGGLGHR+ DCPKLEHQKSMAIA SRRDY
Sbjct: 558 QRIPPVLAELNDPLEDEETMAKESGVKGCAYCGGLGHRVTDCPKLEHQKSMAIAGSRRDY 617

BLAST of Carg23133 vs. ExPASy Swiss-Prot
Match: Q5Z6G5 (DEAD-box ATP-dependent RNA helicase 35B OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0697200 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 7.4e-261
Identity = 454/599 (75.79%), Postives = 520/599 (86.81%), Query Frame = 0

Query: 14  EEEDYVEYVPVAKRRAMEAQKILMRKGK-------ASALEEELEKSRLAEA------KPS 73
           +EEDY EYVPVAKRRAMEA++ L R  K       A A      +S  + A      K S
Sbjct: 22  DEEDYEEYVPVAKRRAMEAER-LRRATKPPTTNAVAVAAPPPPPRSTSSPAVGEVAVKTS 81

Query: 74  LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP 133
           LLVKA+++KR+ PEV+P E+++QQE+EMIEHLSDRK LM V E+AKGI+Y+EP+ TGW+P
Sbjct: 82  LLVKATKLKREAPEVTPAERLLQQEREMIEHLSDRKALMPVGEIAKGISYSEPITTGWRP 141

Query: 134 PLPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ 193
           PL +RRMPR   D +R+ WHI+VDGDD+PPP ++F D+RLPEP+L+ L+ KGI +PTPIQ
Sbjct: 142 PLRLRRMPRSRADALRRSWHILVDGDDVPPPSRSFGDLRLPEPILRALRGKGIEKPTPIQ 201

Query: 194 VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE 253
           VQGLPV LSGRDMIGIAFTGSGKTLVFVLPLIM ALQEE++MPIV GEGPFGLI+CPSRE
Sbjct: 202 VQGLPVALSGRDMIGIAFTGSGKTLVFVLPLIMAALQEEILMPIVPGEGPFGLIVCPSRE 261

Query: 254 LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML 313
           LARQT+EV+E FL P+ EAGYPE+RPLLCIGGVDMR+Q+E+VKKGVHIVVATPGRLKD+L
Sbjct: 262 LARQTHEVIEMFLAPLMEAGYPEIRPLLCIGGVDMRTQMEVVKKGVHIVVATPGRLKDLL 321

Query: 314 AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS 373
           +KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMP KIQNFA+S
Sbjct: 322 SKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPEKIQNFAKS 381

Query: 374 ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI 433
           ALVKP+ VNVGRAGAANLDVIQEVEYVK+EA+I+YLLECLQKTPPPVL+FCE+KADVD I
Sbjct: 382 ALVKPIIVNVGRAGAANLDVIQEVEYVKEEARIIYLLECLQKTPPPVLVFCEHKADVDYI 441

Query: 434 HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD 493
            E+LLLKGVEAVAIHGGKD EER+ A  SFKAS+KDVLVATDVASKGLD PDIQHVINYD
Sbjct: 442 QEFLLLKGVEAVAIHGGKDDEERKDAFKSFKASEKDVLVATDVASKGLDIPDIQHVINYD 501

Query: 494 MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND 553
           MPAEIENYVHRIGRTGR GKTG+ATTFINKNQ+ETTLLDLK LL E+KQR+PP+LA+L+D
Sbjct: 502 MPAEIENYVHRIGRTGRRGKTGVATTFINKNQTETTLLDLKQLLIESKQRLPPILADLDD 561

Query: 554 PMEDVE-AITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 599
           P ED + AI   SGVKGCA+CGGLGHRI  CPK + Q S+ +A +R DYFG GGYRGEI
Sbjct: 562 PQEDDKVAIAQQSGVKGCAFCGGLGHRIEACPKQQLQNSVTLARARSDYFGGGGYRGEI 619

BLAST of Carg23133 vs. ExPASy Swiss-Prot
Match: Q9SZB4 (Putative DEAD-box ATP-dependent RNA helicase 43 OS=Arabidopsis thaliana OX=3702 GN=RH43 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 3.5e-255
Identity = 445/587 (75.81%), Postives = 488/587 (83.13%), Query Frame = 0

Query: 12  MEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSLLVKASQMKRD 71
           ME ++ YVEYVPV +R A   +K++   GK                              
Sbjct: 1   MEVDDGYVEYVPVEERLAQMKRKVVEEPGKG----------------------------- 60

Query: 72  QPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPPLPIRRMPRKA 131
                           M+EHLSD+K LMSV ELA+GITYTEPL T WKPPL +R+M  K 
Sbjct: 61  ----------------MMEHLSDKKKLMSVGELARGITYTEPLSTWWKPPLHVRKMSTKQ 120

Query: 132 CDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQVQGLPVILSGR 191
            DLIRKQWHI V+G+DIPPPIKNF DM+ P P+L+ LK+KGI+ PTPIQVQGLPV+LSGR
Sbjct: 121 MDLIRKQWHITVNGEDIPPPIKNFMDMKFPSPLLRMLKDKGIMHPTPIQVQGLPVVLSGR 180

Query: 192 DMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTYEVVEQ 251
           DMIGIAFTGSGKTLVFVLP+I++ALQEE+MMPI +GEGP  L+ICPSRELA+QTY+VVEQ
Sbjct: 181 DMIGIAFTGSGKTLVFVLPMIILALQEEIMMPIAAGEGPIALVICPSRELAKQTYDVVEQ 240

Query: 252 FLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLAKKKMNLDNCR 311
           F+  + E GYP LR LLCIGGVDMRSQ+++VKKGVHIVVATPGRLKD+LAKKKM+LD CR
Sbjct: 241 FVASLVEDGYPRLRSLLCIGGVDMRSQLDVVKKGVHIVVATPGRLKDILAKKKMSLDACR 300

Query: 312 YLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPVTVNVG 371
            LTLDEADRLVDLGFEDDIR VFDHFK+QRQTLLFSATMP KIQ FA SALVKPVTVNVG
Sbjct: 301 LLTLDEADRLVDLGFEDDIRHVFDHFKSQRQTLLFSATMPAKIQIFATSALVKPVTVNVG 360

Query: 372 RAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLLKGVEA 431
           RAGAANLDVIQEVEYVKQEAKIVYLLECLQKT PPVLIFCENKADVDDIHEYLLLKGVEA
Sbjct: 361 RAGAANLDVIQEVEYVKQEAKIVYLLECLQKTTPPVLIFCENKADVDDIHEYLLLKGVEA 420

Query: 432 VAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENYVHR 491
           VAIHGGKDQE+R+YAIS FKA KKDVLVATDVASKGLDFPDIQHVINYDMP EIENYVHR
Sbjct: 421 VAIHGGKDQEDRDYAISLFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPGEIENYVHR 480

Query: 492 IGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVEAITNA 551
           IGRTGRCGKTGIATTFINKNQSE TLLDLKHLLQEAKQRIPPVLAELN PME+ E I NA
Sbjct: 481 IGRTGRCGKTGIATTFINKNQSEITLLDLKHLLQEAKQRIPPVLAELNGPMEETETIANA 540

Query: 552 SGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 599
           SGVKGCAYCGGLGHRI  CPK EHQKS+AI+SSR+D+FGS GYRGE+
Sbjct: 541 SGVKGCAYCGGLGHRILQCPKFEHQKSVAISSSRKDHFGSDGYRGEV 542

BLAST of Carg23133 vs. ExPASy Swiss-Prot
Match: Q9UJV9 (Probable ATP-dependent RNA helicase DDX41 OS=Homo sapiens OX=9606 GN=DDX41 PE=1 SV=2)

HSP 1 Score: 695.7 bits (1794), Expect = 4.7e-199
Identity = 357/596 (59.90%), Postives = 456/596 (76.51%), Query Frame = 0

Query: 7   KDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRL-----------A 66
           + E   E++EDYV YVP+ +RR +  QK+L R+ K +A EE+ +                
Sbjct: 22  RSEAEDEDDEDYVPYVPLRQRRQLLLQKLLQRRRKGAAEEEQQDSGSEPRGDEDDIPLGP 81

Query: 67  EAKPSLLVKASQMKR--DQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEP 126
           ++  SLL +   +K   +  + S  E+ +++E++++E +++ + LMSV+E+AKGITY +P
Sbjct: 82  QSNVSLLDQHQHLKEKAEARKESAKEKQLKEEEKILESVAEGRALMSVKEMAKGITYDDP 141

Query: 127 LLTGWKPPLPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGI 186
           + T W PP  +  M  +  + +RK++HI+V+GD IPPPIK+FK+M+ P  +L+ LK+KGI
Sbjct: 142 IKTSWTPPRYVLSMSEERHERVRKKYHILVEGDGIPPPIKSFKEMKFPAAILRGLKKKGI 201

Query: 187 VQPTPIQVQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGL 246
             PTPIQ+QG+P ILSGRDMIGIAFTGSGKTLVF LP+IM  L++E  +P    EGP+GL
Sbjct: 202 HHPTPIQIQGIPTILSGRDMIGIAFTGSGKTLVFTLPVIMFCLEQEKRLPFSKREGPYGL 261

Query: 247 IICPSRELARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATP 306
           IICPSRELARQT+ ++E +   ++E   P LR  LCIGG+ ++ Q+E ++ GVH++VATP
Sbjct: 262 IICPSRELARQTHGILEYYCRLLQEDSSPLLRCALCIGGMSVKEQMETIRHGVHMMVATP 321

Query: 307 GRLKDMLAKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTK 366
           GRL D+L KK ++LD CRYL LDEADR++D+GFE DIR +F +FK QRQTLLFSATMP K
Sbjct: 322 GRLMDLLQKKMVSLDICRYLALDEADRMIDMGFEGDIRTIFSYFKGQRQTLLFSATMPKK 381

Query: 367 IQNFARSALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCEN 426
           IQNFA+SALVKPVT+NVGRAGAA+LDVIQEVEYVK+EAK+VYLLECLQKTPPPVLIF E 
Sbjct: 382 IQNFAKSALVKPVTINVGRAGAASLDVIQEVEYVKEEAKMVYLLECLQKTPPPVLIFAEK 441

Query: 427 KADVDDIHEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDI 486
           KADVD IHEYLLLKGVEAVAIHGGKDQEER  AI +F+  KKDVLVATDVASKGLDFP I
Sbjct: 442 KADVDAIHEYLLLKGVEAVAIHGGKDQEERTKAIEAFREGKKDVLVATDVASKGLDFPAI 501

Query: 487 QHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPP 546
           QHVINYDMP EIENYVHRIGRTGR G TGIATTFINK   E+ L+DLK LL EAKQ++PP
Sbjct: 502 QHVINYDMPEEIENYVHRIGRTGRSGNTGIATTFINKACDESVLMDLKALLLEAKQKVPP 561

Query: 547 VLAELNDPMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIAS-SRRDY 589
           VL  L+      E++ +  G +GCA+CGGLGHRI DCPKLE  ++  +++  R+DY
Sbjct: 562 VLQVLHC---GDESMLDIGGERGCAFCGGLGHRITDCPKLEAMQTKQVSNIGRKDY 614

BLAST of Carg23133 vs. ExPASy TrEMBL
Match: A0A6J1KU58 (RNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111497657 PE=3 SV=1)

HSP 1 Score: 1172.1 bits (3031), Expect = 0.0e+00
Identity = 598/598 (100.00%), Postives = 598/598 (100.00%), Query Frame = 0

Query: 1   MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPS 60
           MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPS
Sbjct: 1   MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPS 60

Query: 61  LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP 120
           LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP
Sbjct: 61  LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP 120

Query: 121 PLPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ 180
           PLPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ
Sbjct: 121 PLPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ 180

Query: 181 VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE 240
           VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE
Sbjct: 181 VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE 240

Query: 241 LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML 300
           LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML
Sbjct: 241 LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML 300

Query: 301 AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS 360
           AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS
Sbjct: 301 AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS 360

Query: 361 ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI 420
           ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI
Sbjct: 361 ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI 420

Query: 421 HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD 480
           HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD
Sbjct: 421 HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD 480

Query: 481 MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND 540
           MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND
Sbjct: 481 MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND 540

Query: 541 PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 599
           PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 541 PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598

BLAST of Carg23133 vs. ExPASy TrEMBL
Match: A0A6J1H714 (RNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111461026 PE=3 SV=1)

HSP 1 Score: 1170.2 bits (3026), Expect = 0.0e+00
Identity = 597/598 (99.83%), Postives = 598/598 (100.00%), Query Frame = 0

Query: 1   MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPS 60
           MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPS
Sbjct: 1   MMDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPS 60

Query: 61  LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP 120
           LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP
Sbjct: 61  LLVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP 120

Query: 121 PLPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ 180
           PLPIRRMPRKACDLIRKQWHIIVDGD+IPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ
Sbjct: 121 PLPIRRMPRKACDLIRKQWHIIVDGDNIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQ 180

Query: 181 VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE 240
           VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE
Sbjct: 181 VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE 240

Query: 241 LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML 300
           LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML
Sbjct: 241 LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDML 300

Query: 301 AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS 360
           AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS
Sbjct: 301 AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS 360

Query: 361 ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI 420
           ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI
Sbjct: 361 ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI 420

Query: 421 HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD 480
           HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD
Sbjct: 421 HEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYD 480

Query: 481 MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND 540
           MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND
Sbjct: 481 MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND 540

Query: 541 PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 599
           PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 541 PMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598

BLAST of Carg23133 vs. ExPASy TrEMBL
Match: A0A1S3CDK5 (RNA helicase OS=Cucumis melo OX=3656 GN=LOC103499818 PE=3 SV=1)

HSP 1 Score: 1162.9 bits (3007), Expect = 0.0e+00
Identity = 591/597 (98.99%), Postives = 596/597 (99.83%), Query Frame = 0

Query: 2   MDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL 61
           MDV MKDEKS+EEE+DYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL
Sbjct: 1   MDVQMKDEKSLEEEDDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL 60

Query: 62  LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 121
           LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP
Sbjct: 61  LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120

Query: 122 LPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV 181
           LPIRRMP+KACDLIRKQWHIIVDGD+IPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV
Sbjct: 121 LPIRRMPKKACDLIRKQWHIIVDGDEIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV 180

Query: 182 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 241
           QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL
Sbjct: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240

Query: 242 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLA 301
           ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIE+VKKGVHIVVATPGRLKDMLA
Sbjct: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300

Query: 302 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 361
           KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA
Sbjct: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360

Query: 362 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 421
           LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH
Sbjct: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420

Query: 422 EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM 481
           EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM
Sbjct: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM 480

Query: 482 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 541
           PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP
Sbjct: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540

Query: 542 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 599
           MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 597

BLAST of Carg23133 vs. ExPASy TrEMBL
Match: A0A5A7UYT9 (RNA helicase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G002050 PE=3 SV=1)

HSP 1 Score: 1162.9 bits (3007), Expect = 0.0e+00
Identity = 591/597 (98.99%), Postives = 596/597 (99.83%), Query Frame = 0

Query: 2   MDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL 61
           MDV MKDEKS+EEE+DYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL
Sbjct: 1   MDVQMKDEKSLEEEDDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL 60

Query: 62  LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 121
           LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP
Sbjct: 61  LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120

Query: 122 LPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV 181
           LPIRRMP+KACDLIRKQWHIIVDGD+IPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV
Sbjct: 121 LPIRRMPKKACDLIRKQWHIIVDGDEIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV 180

Query: 182 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 241
           QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL
Sbjct: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240

Query: 242 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLA 301
           ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIE+VKKGVHIVVATPGRLKDMLA
Sbjct: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300

Query: 302 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 361
           KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA
Sbjct: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360

Query: 362 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 421
           LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH
Sbjct: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420

Query: 422 EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM 481
           EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM
Sbjct: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM 480

Query: 482 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 541
           PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP
Sbjct: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540

Query: 542 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 599
           MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 597

BLAST of Carg23133 vs. ExPASy TrEMBL
Match: A0A0A0K6A2 (RNA helicase OS=Cucumis sativus OX=3659 GN=Csa_7G432260 PE=3 SV=1)

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 590/597 (98.83%), Postives = 596/597 (99.83%), Query Frame = 0

Query: 2   MDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL 61
           MDV MKDEKS+EEE++YVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL
Sbjct: 121 MDVQMKDEKSLEEEDEYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL 180

Query: 62  LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 121
           LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP
Sbjct: 181 LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 240

Query: 122 LPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV 181
           LPIRRMP+KACDLIRKQWHIIVDGD+IPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV
Sbjct: 241 LPIRRMPKKACDLIRKQWHIIVDGDEIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV 300

Query: 182 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 241
           QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL
Sbjct: 301 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 360

Query: 242 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLA 301
           ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIE+VKKGVHIVVATPGRLKDMLA
Sbjct: 361 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 420

Query: 302 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 361
           KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA
Sbjct: 421 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 480

Query: 362 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 421
           LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH
Sbjct: 481 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 540

Query: 422 EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM 481
           EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM
Sbjct: 541 EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM 600

Query: 482 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 541
           PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP
Sbjct: 601 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 660

Query: 542 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 599
           MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 661 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 717

BLAST of Carg23133 vs. TAIR 10
Match: AT5G51280.1 (DEAD-box protein abstrakt, putative )

HSP 1 Score: 1036.6 bits (2679), Expect = 8.0e-303
Identity = 521/590 (88.31%), Postives = 555/590 (94.07%), Query Frame = 0

Query: 9   EKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSLLVKASQM 68
           E  MEE + Y+EYV VA+RRA+ AQKIL RKGKAS LEEE +K +LAEAKPSLLV+A+Q+
Sbjct: 2   ESIMEEADSYIEYVSVAERRAIAAQKILQRKGKASELEEEADKEKLAEAKPSLLVQATQL 61

Query: 69  KRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPPLPIRRMP 128
           KRD PEVS TEQI+ QEKEM+EHLSD+KTLMSVRELAKGITYTEPLLTGWKPPL IR+M 
Sbjct: 62  KRDVPEVSATEQIILQEKEMMEHLSDKKTLMSVRELAKGITYTEPLLTGWKPPLHIRKMS 121

Query: 129 RKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQVQGLPVIL 188
            K  DLIRKQWHIIV+GDDIPPPIKNFKDM+ P PVL  LKEKGIVQPTPIQVQGLPVIL
Sbjct: 122 SKQRDLIRKQWHIIVNGDDIPPPIKNFKDMKFPRPVLDTLKEKGIVQPTPIQVQGLPVIL 181

Query: 189 SGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTYEV 248
           +GRDMIGIAFTGSGKTLVFVLP+IMIALQEE+MMPI +GEGP GLI+CPSRELARQTYEV
Sbjct: 182 AGRDMIGIAFTGSGKTLVFVLPMIMIALQEEMMMPIAAGEGPIGLIVCPSRELARQTYEV 241

Query: 249 VEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLAKKKMNLD 308
           VEQF+ P+ EAGYP LR LLCIGG+DMRSQ+E+VK+GVHIVVATPGRLKDMLAKKKM+LD
Sbjct: 242 VEQFVAPLVEAGYPPLRSLLCIGGIDMRSQLEVVKRGVHIVVATPGRLKDMLAKKKMSLD 301

Query: 309 NCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPVTV 368
            CRYLTLDEADRLVDLGFEDDIREVFDHFK+QRQTLLFSATMPTKIQ FARSALVKPVTV
Sbjct: 302 ACRYLTLDEADRLVDLGFEDDIREVFDHFKSQRQTLLFSATMPTKIQIFARSALVKPVTV 361

Query: 369 NVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLLKG 428
           NVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKT PPVLIFCENKADVDDIHEYLLLKG
Sbjct: 362 NVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTSPPVLIFCENKADVDDIHEYLLLKG 421

Query: 429 VEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENY 488
           VEAVAIHGGKDQE+REYAISSFKA KKDVLVATDVASKGLDFPDIQHVINYDMPAEIENY
Sbjct: 422 VEAVAIHGGKDQEDREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENY 481

Query: 489 VHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVEAI 548
           VHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPME+ E I
Sbjct: 482 VHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEEAETI 541

Query: 549 TNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 599
            NASGVKGCAYCGGLGHRIRDCPKLEHQKS+AI++SR+DYFGSGGYRGEI
Sbjct: 542 ANASGVKGCAYCGGLGHRIRDCPKLEHQKSVAISNSRKDYFGSGGYRGEI 591

BLAST of Carg23133 vs. TAIR 10
Match: AT4G33370.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 882.1 bits (2278), Expect = 2.5e-256
Identity = 445/587 (75.81%), Postives = 488/587 (83.13%), Query Frame = 0

Query: 12  MEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSLLVKASQMKRD 71
           ME ++ YVEYVPV +R A   +K++   GK                              
Sbjct: 1   MEVDDGYVEYVPVEERLAQMKRKVVEEPGKG----------------------------- 60

Query: 72  QPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPPLPIRRMPRKA 131
                           M+EHLSD+K LMSV ELA+GITYTEPL T WKPPL +R+M  K 
Sbjct: 61  ----------------MMEHLSDKKKLMSVGELARGITYTEPLSTWWKPPLHVRKMSTKQ 120

Query: 132 CDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQVQGLPVILSGR 191
            DLIRKQWHI V+G+DIPPPIKNF DM+ P P+L+ LK+KGI+ PTPIQVQGLPV+LSGR
Sbjct: 121 MDLIRKQWHITVNGEDIPPPIKNFMDMKFPSPLLRMLKDKGIMHPTPIQVQGLPVVLSGR 180

Query: 192 DMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTYEVVEQ 251
           DMIGIAFTGSGKTLVFVLP+I++ALQEE+MMPI +GEGP  L+ICPSRELA+QTY+VVEQ
Sbjct: 181 DMIGIAFTGSGKTLVFVLPMIILALQEEIMMPIAAGEGPIALVICPSRELAKQTYDVVEQ 240

Query: 252 FLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLAKKKMNLDNCR 311
           F+  + E GYP LR LLCIGGVDMRSQ+++VKKGVHIVVATPGRLKD+LAKKKM+LD CR
Sbjct: 241 FVASLVEDGYPRLRSLLCIGGVDMRSQLDVVKKGVHIVVATPGRLKDILAKKKMSLDACR 300

Query: 312 YLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPVTVNVG 371
            LTLDEADRLVDLGFEDDIR VFDHFK+QRQTLLFSATMP KIQ FA SALVKPVTVNVG
Sbjct: 301 LLTLDEADRLVDLGFEDDIRHVFDHFKSQRQTLLFSATMPAKIQIFATSALVKPVTVNVG 360

Query: 372 RAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLLKGVEA 431
           RAGAANLDVIQEVEYVKQEAKIVYLLECLQKT PPVLIFCENKADVDDIHEYLLLKGVEA
Sbjct: 361 RAGAANLDVIQEVEYVKQEAKIVYLLECLQKTTPPVLIFCENKADVDDIHEYLLLKGVEA 420

Query: 432 VAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENYVHR 491
           VAIHGGKDQE+R+YAIS FKA KKDVLVATDVASKGLDFPDIQHVINYDMP EIENYVHR
Sbjct: 421 VAIHGGKDQEDRDYAISLFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPGEIENYVHR 480

Query: 492 IGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVEAITNA 551
           IGRTGRCGKTGIATTFINKNQSE TLLDLKHLLQEAKQRIPPVLAELN PME+ E I NA
Sbjct: 481 IGRTGRCGKTGIATTFINKNQSEITLLDLKHLLQEAKQRIPPVLAELNGPMEETETIANA 540

Query: 552 SGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 599
           SGVKGCAYCGGLGHRI  CPK EHQKS+AI+SSR+D+FGS GYRGE+
Sbjct: 541 SGVKGCAYCGGLGHRILQCPKFEHQKSVAISSSRKDHFGSDGYRGEV 542

BLAST of Carg23133 vs. TAIR 10
Match: AT1G55150.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 303.9 bits (777), Expect = 2.8e-82
Identity = 163/406 (40.15%), Postives = 246/406 (60.59%), Query Frame = 0

Query: 136 RKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQVQGLPVILSGRDMIG 195
           RK   I V+G DIP P+K+F+D+  P+ VL+++K+ G  +PTPIQ QG P+ + GRD+IG
Sbjct: 82  RKLREITVEGKDIPKPVKSFRDVGFPDYVLEEVKKAGFTEPTPIQSQGWPMAMKGRDLIG 141

Query: 196 IAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTYEVVEQFLIP 255
           IA TGSGKTL ++LP I+    + +   +  G+GP  L++ P+RELA Q  +   +F   
Sbjct: 142 IAETGSGKTLSYLLPAIVHVNAQPM---LAHGDGPIVLVLAPTRELAVQIQQEASKF--- 201

Query: 256 MKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLAKKKMNLDNCRYLTL 315
                  +++     GGV    Q+  ++KGV IV+ATPGRL DM+     NL    YL L
Sbjct: 202 ---GSSSKIKTTCIYGGVPKGPQVRDLQKGVEIVIATPGRLIDMMESNNTNLRRVTYLVL 261

Query: 316 DEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPVTVNVGRAG- 375
           DEADR++D+GF+  IR++  H +  RQTL +SAT P +++  ++  L  P  V +G +  
Sbjct: 262 DEADRMLDMGFDPQIRKIVSHIRPDRQTLYWSATWPKEVEQLSKKFLYNPYKVIIGSSDL 321

Query: 376 AANLDVIQEVEYVKQEAKIVYLLECLQ--KTPPPVLIFCENKADVDDIHEYLLLKGVEAV 435
            AN  + Q V+ + +  K   L++ L+       +L+F + K   D I   L + G  A+
Sbjct: 322 KANRAIRQIVDVISESQKYNKLVKLLEDIMDGSRILVFLDTKKGCDQITRQLRMDGWPAL 381

Query: 436 AIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENYVHRI 495
           +IHG K Q ER++ +S F++ K  ++ ATDVA++GLD  D+++VINYD P  +E+YVHRI
Sbjct: 382 SIHGDKSQAERDWVLSEFRSGKSPIMTATDVAARGLDVKDVKYVINYDFPGSLEDYVHRI 441

Query: 496 GRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAEL 539
           GRTGR G  G A TF     +     +L ++LQEA Q++ P LA +
Sbjct: 442 GRTGRAGAKGTAYTFFTVANARFA-KELTNILQEAGQKVSPELASM 477

BLAST of Carg23133 vs. TAIR 10
Match: AT2G47330.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 296.2 bits (757), Expect = 5.9e-80
Identity = 175/425 (41.18%), Postives = 245/425 (57.65%), Query Frame = 0

Query: 136 RKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQVQGLPVILSGRDMIG 195
           R++  I V G D+  P+K F+D      ++  +K++   +PT IQ Q LP++LSGRD+IG
Sbjct: 211 RQRLGIRVSGFDVHRPVKTFEDCGFSSQIMSAIKKQAYEKPTAIQCQALPIVLSGRDVIG 270

Query: 196 IAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTYEVVEQFLIP 255
           IA TGSGKT  FVLP+I+  + +  +      EGP G+I  P+RELA Q +   ++F   
Sbjct: 271 IAKTGSGKTAAFVLPMIVHIMDQPELQ---RDEGPIGVICAPTRELAHQIFLEAKKF--- 330

Query: 256 MKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLAKKKMNLDNCRYLTL 315
            K  G   LR     GG+    Q + +K G  IVVATPGRL DML  K + +    YL L
Sbjct: 331 SKAYG---LRVSAVYGGMSKHEQFKELKAGCEIVVATPGRLIDMLKMKALTMMRASYLVL 390

Query: 316 DEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPVTVNVGRAGA 375
           DEADR+ DLGFE  +R +    +  RQTLLFSATMP K++  AR  L  P+ V VG  G 
Sbjct: 391 DEADRMFDLGFEPQVRSIVGQIRPDRQTLLFSATMPWKVEKLAREILSDPIRVTVGEVGM 450

Query: 376 ANLDVIQEVEYVKQEA-KIVYLLECL--QKTPPPVLIFCENKADVDDIHEYLLLKGVEAV 435
           AN D+ Q V  +  +A K+ +LLE L        VL+F   KA VD+I   L L   +  
Sbjct: 451 ANEDITQVVNVIPSDAEKLPWLLEKLPGMIDEGDVLVFASKKATVDEIEAQLTLNSFKVA 510

Query: 436 AIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENYVHRI 495
           A+HG KDQ  R   +  FK+    VL+ATDVA++GLD   ++ V+NYD+  +++ +VHRI
Sbjct: 511 ALHGDKDQASRMETLQKFKSGVHHVLIATDVAARGLDIKSLKTVVNYDIAKDMDMHVHRI 570

Query: 496 GRTGRCG-KTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVEAITNA 555
           GRTGR G + G+A T + + ++     +L + L  A Q +PP L +L   M+D    +  
Sbjct: 571 GRTGRAGDRDGVAYTLVTQREARFA-GELVNSLVAAGQNVPPELTDL--AMKDGRFKSKR 623

Query: 556 SGVKG 557
            G KG
Sbjct: 631 DGRKG 623

BLAST of Carg23133 vs. TAIR 10
Match: AT5G63120.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 294.7 bits (753), Expect = 1.7e-79
Identity = 170/419 (40.57%), Postives = 246/419 (58.71%), Query Frame = 0

Query: 124 IRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQVQG 183
           ++ M  +   + R +  I V+G D+P P+K F+D   P+ +L+ + + G  +PTPIQ QG
Sbjct: 136 VQAMTEQDVAMYRTERDISVEGRDVPKPMKMFQDANFPDNILEAIAKLGFTEPTPIQAQG 195

Query: 184 LPVILSGRDMIGIAFTGSGKTLVFVLP-LIMIALQEEVMMPIVSGEGPFGLIICPSRELA 243
            P+ L GRD+IGIA TGSGKTL ++LP L+ ++ Q      +   +GP  LI+ P+RELA
Sbjct: 196 WPMALKGRDLIGIAETGSGKTLAYLLPALVHVSAQPR----LGQDDGPIVLILAPTRELA 255

Query: 244 RQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLAK 303
            Q  E   +F +    +G   +R     GG     QI  +++GV IV+ATPGRL DML  
Sbjct: 256 VQIQEESRKFGL---RSG---VRSTCIYGGAPKGPQIRDLRRGVEIVIATPGRLIDMLEC 315

Query: 304 KKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSAL 363
           +  NL    YL LDEADR++D+GFE  IR++    +  RQTLL+SAT P +++  AR  L
Sbjct: 316 QHTNLKRVTYLVLDEADRMLDMGFEPQIRKIVSQIRPDRQTLLWSATWPREVETLARQFL 375

Query: 364 VKPVTVNVGRAG-AANLDVIQEVEYVKQEAKIVYLLECLQK--TPPPVLIFCENKADVDD 423
             P    +G     AN  + Q +E V    K   LL  L++      +LIF E K   D 
Sbjct: 376 RDPYKAIIGSTDLKANQSINQVIEIVPTPEKYNRLLTLLKQLMDGSKILIFVETKRGCDQ 435

Query: 424 IHEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINY 483
           +   L + G  A+AIHG K Q ER+  ++ FK+ +  ++ ATDVA++GLD  DI+ V+NY
Sbjct: 436 VTRQLRMDGWPALAIHGDKTQSERDRVLAEFKSGRSPIMTATDVAARGLDVKDIKCVVNY 495

Query: 484 DMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAEL 539
           D P  +E+Y+HRIGRTGR G  G+A TF   + ++    +L  +LQEA Q +PP L+ L
Sbjct: 496 DFPNTLEDYIHRIGRTGRAGAKGMAFTFFTHDNAKFA-RELVKILQEAGQVVPPTLSAL 543

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023004285.10.0e+00100.00DEAD-box ATP-dependent RNA helicase 35 [Cucurbita maxima] >XP_023004286.1 DEAD-b... [more]
KAG6593383.10.0e+00100.00DEAD-box ATP-dependent RNA helicase 35, partial [Cucurbita argyrosperma subsp. s... [more]
XP_022960221.10.0e+0099.83DEAD-box ATP-dependent RNA helicase 35-like [Cucurbita moschata] >XP_022960222.1... [more]
XP_008461142.10.0e+0098.99PREDICTED: DEAD-box ATP-dependent RNA helicase 35 [Cucumis melo] >XP_008461143.1... [more]
XP_038885065.10.0e+0098.66DEAD-box ATP-dependent RNA helicase 35 [Benincasa hispida] >XP_038885066.1 DEAD-... [more]
Match NameE-valueIdentityDescription
Q9LU461.1e-30188.31DEAD-box ATP-dependent RNA helicase 35 OS=Arabidopsis thaliana OX=3702 GN=RH35 P... [more]
Q0E3X44.0e-28379.84DEAD-box ATP-dependent RNA helicase 35A OS=Oryza sativa subsp. japonica OX=39947... [more]
Q5Z6G57.4e-26175.79DEAD-box ATP-dependent RNA helicase 35B OS=Oryza sativa subsp. japonica OX=39947... [more]
Q9SZB43.5e-25575.81Putative DEAD-box ATP-dependent RNA helicase 43 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9UJV94.7e-19959.90Probable ATP-dependent RNA helicase DDX41 OS=Homo sapiens OX=9606 GN=DDX41 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1KU580.0e+00100.00RNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111497657 PE=3 SV=1[more]
A0A6J1H7140.0e+0099.83RNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111461026 PE=3 SV=1[more]
A0A1S3CDK50.0e+0098.99RNA helicase OS=Cucumis melo OX=3656 GN=LOC103499818 PE=3 SV=1[more]
A0A5A7UYT90.0e+0098.99RNA helicase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G002050 ... [more]
A0A0A0K6A20.0e+0098.83RNA helicase OS=Cucumis sativus OX=3659 GN=Csa_7G432260 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G51280.18.0e-30388.31DEAD-box protein abstrakt, putative [more]
AT4G33370.12.5e-25675.81DEA(D/H)-box RNA helicase family protein [more]
AT1G55150.12.8e-8240.15DEA(D/H)-box RNA helicase family protein [more]
AT2G47330.15.9e-8041.18P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G63120.21.7e-7940.57P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 510..530
NoneNo IPR availablePANTHERPTHR47958:SF79BNAA06G38640D PROTEINcoord: 19..578
NoneNo IPR availablePANTHERPTHR47958ATP-DEPENDENT RNA HELICASE DBP3coord: 19..578
NoneNo IPR availableCDDcd18787SF2_C_DEADcoord: 380..508
e-value: 3.75783E-56
score: 183.863
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 172..383
e-value: 2.2E-56
score: 203.3
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 184..368
score: 30.383554
IPR001650Helicase, C-terminalSMARTSM00490helicmild6coord: 418..499
e-value: 5.5E-33
score: 125.6
IPR001650Helicase, C-terminalPFAMPF00271Helicase_Ccoord: 392..499
e-value: 3.9E-30
score: 104.5
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 379..539
score: 23.096851
IPR001878Zinc finger, CCHC-typeSMARTSM00343c2hcfinal6coord: 556..572
e-value: 0.0053
score: 25.3
IPR001878Zinc finger, CCHC-typePFAMPF00098zf-CCHCcoord: 556..572
e-value: 2.0E-4
score: 21.3
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 557..572
score: 9.108787
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 122..372
e-value: 6.9E-80
score: 270.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 378..540
e-value: 1.9E-55
score: 188.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 229..514
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 177..356
e-value: 5.1E-48
score: 163.2
IPR014014RNA helicase, DEAD-box type, Q motifPROSITEPS51195Q_MOTIFcoord: 153..181
score: 10.800855
IPR044113DDX41, DEAD-box helicase domainCDDcd17951DEADc_DDX41coord: 164..369
e-value: 1.06411E-137
score: 397.095
IPR036875Zinc finger, CCHC-type superfamilySUPERFAMILY57756Retrovirus zinc finger-like domainscoord: 556..577

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23133-RACarg23133-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0005681 spliceosomal complex
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0003724 RNA helicase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003676 nucleic acid binding