Carg23052 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg23052
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	hemK methyltransferase family member 2-like
Location	Carg_Chr11: 9293799 .. 9296065 (-)
RNA-Seq Expression	Carg23052
Synteny	Carg23052

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGGTCTCTCATTTCCTCGCAGCAATTTCCTCGTACCCTCCGTTCTCCTTCCTGGTATTGTATCCATTTCCTTTGATTTCAGTTGCTTTCTTTCCTTTTTGATGGATGTTGAGTATAATCGCTTAATTCTACTGATTTTCTCCGAGCCTCCATGAATCGAAATGAAGCTGGCTTGTGTAAAATCTATTGGCTGTTGTTTATGTTCATGTGCCGAATCGTGTAGTTTGATTCGCTTTATGAATCCTGGAGTCTTCTTGACTTGTTTTATCTCTCATTTTCTTGCTTGTGTTCTTTCGGAAATCAAAAATCGGTGTCCCATTGTTGATTAAAGTGAAGGTTCTGATCCCTACTTGTAGGAACATTTTGTGCCCTAAAATCACTGGGAATTGCTCGGAAATTATCCGGGAACCGTCTGCGAAAAGAATTATTGGGATTTGGGCCTGAATTTCTGCCACTGACTTTTTTGGTGGCCTTGCTCGGGATTGTTGGGAGGGAGTTCTGCATTAGCTAATTTAGGCAACGATCATGGGCTTACATCTCTATTGGTATGAGGCCTTTTGGGGAAGCTCAAAGCAAAACCATGAGAGCTTATACTCAAAGTGAATAATATCATAACATTGTGGAGAGTCGTGATTTCTAACATGGTATTAGAGTCATGCTCTTAACTTAGTCATCTCAATAGCATCCTCAAATGTCAGAACCAAGAAGTTGTAAGCCTCGAAAGTGTAGTCAAAAGTGACTCAAGTGTCGACAAAAGGTGTACTTTGTTCGAGGACTCCAGAGAAGGAGTCAAGCCTTGATTAAGAGGAGACTATTTGAGGGCTCCATAGGCCTCGGGGGAGGCTCTATAGTGTACTTTGTTCGAGGGGAGGTGAGGAATACATCTCCATTGGTTTGAGGCCTTTTGGAGAAGTCCAAAGCAAAACCATGAGAGCTTATGCTCAAGGTGGACAGTATCATACCATTGTGGAGAGTCGTTATTCCTAACAAGTCTTTTCAATATCGATGCAATTGTTCTCGTAATATTACACACACACAAGTTTACTTATACTTGTTCATCTCTTCTTATCCATAATTTCGTGACAGTTCCAGCACATGTCCCCATTCAAAACTGCGCAAATCCACCTTGTGAGTTCTCATCGTGAGGTTTATGAACCCTGTGATGATTCATTTGCTTTGGTTGATGCACTTTTAGCTGACCGAGTTAACTTGTTAAATCATCACCCAACATTGTGCTTAGAAGTAGGCTGTGGAAGTGGGTATGTCATAACATCTCTTGCTCTTATGTTGGGAAAGGAAGCCTCTGGCACTCACTATATCGCTACAGACATCAATCCTCATGCAACGAGAGTCACTCAGGAGACACTAGAAGCACATGGGGTTCATGCGGAGTTGGTATCCACTGACATAGCATATGGTTTGGAGAAACGACTGGCTTCATCTGTTGATGTGTTGATTGTGAATCCACCCTATGTTCCAACCCCTGAGGAGGAAGTGGGTCTTGAAGGAATTGCCTCTGCTTGGGCAGGAGGGGAAAATGGCCGCAGTGTTATCGATAGAATGTTGCCAGTTGCTGATGCTCTTTTGTCTGACAAAGGCTGGTTGTACATGGTTTTTCTTGAAGCAAACAATCCCTCCCAGATATGCCTTCAAATGAGAGAAAAGGGGTATGCTAGTCGAATCGTTGTGCAGAGATCAACTGAAGAAGAGAGTCTTCATGTGATCAAGTTTTGGAAGAATGCTGATCTTCAAGTTCATGGAAAAGGTTCAAGTCACAAAACAGGAACAATTTCAACATCTTAATTAGGAACACTTTCATAAGTTCCTTTTGTTTCCTGAATGTAAGTGCTTAATCTTGCTAATCCATGCTTTGTTGGTACTAGTTATTACATCGGTTTATAATCTCGGTGGCGTTTCGGTTGTTTTTCTCTTGCAGGACTTGAGATTCATCAGGACATCCTCTGTTGTTACAAGGAACTAGCCCATTTTTCAAAATTTGTGCATTCTACAGGTGCGTATTGTTCGATTTTGTATATCAAATACATTTGTTTTGACCCTGTTACTGCTTAGAGATCTTATAGTAGTCGGTACTCTAATTGTCGATGAGTTCGAAACTTCGAAGTTCGTAGTTCATAGGAACTTATAATTTCATTGTCAAGATACTTCTGAAACTGAAAATATATGAATTGAGCTGGCTGAACGTTTTTGAACTGCCATTGTTGACAAGCTTTACTCAAATTAGTTCTCACCTGATAAGATCCAATTCT

mRNA sequence

TTGGTCTCTCATTTCCTCGCAGCAATTTCCTCGTACCCTCCGTTCTCCTTCCTGTTCCAGCACATGTCCCCATTCAAAACTGCGCAAATCCACCTTGTGAGTTCTCATCGTGAGGTTTATGAACCCTGTGATGATTCATTTGCTTTGGTTGATGCACTTTTAGCTGACCGAGTTAACTTGTTAAATCATCACCCAACATTGTGCTTAGAAGTAGGCTGTGGAAGTGGGTATGTCATAACATCTCTTGCTCTTATGTTGGGAAAGGAAGCCTCTGGCACTCACTATATCGCTACAGACATCAATCCTCATGCAACGAGAGTCACTCAGGAGACACTAGAAGCACATGGGGTTCATGCGGAGTTGGTATCCACTGACATAGCATATGGTTTGGAGAAACGACTGGCTTCATCTGTTGATGTGTTGATTGTGAATCCACCCTATGTTCCAACCCCTGAGGAGGAAGTGGGTCTTGAAGGAATTGCCTCTGCTTGGGCAGGAGGGGAAAATGGCCGCAGTGTTATCGATAGAATGTTGCCAGTTGCTGATGCTCTTTTGTCTGACAAAGGCTGGTTGTACATGGTTTTTCTTGAAGCAAACAATCCCTCCCAGATATGCCTTCAAATGAGAGAAAAGGGGTATGCTAGTCGAATCGTTGTGCAGAGATCAACTGAAGAAGAGAGTCTTCATGTGATCAAGTTTTGGAAGAATGCTGATCTTCAAGTTCATGGAAAAGGACTTGAGATTCATCAGGACATCCTCTGTTGTTACAAGGAACTAGCCCATTTTTCAAAATTTGTGCATTCTACAGGTGCGTATTGTTCGATTTTGTATATCAAATACATTTGTTTTGACCCTGTTACTGCTTAGAGATCTTATAGTAGTCGGTACTCTAATTGTCGATGAGTTCGAAACTTCGAAGTTCGTAGTTCATAGGAACTTATAATTTCATTGTCAAGATACTTCTGAAACTGAAAATATATGAATTGAGCTGGCTGAACGTTTTTGAACTGCCATTGTTGACAAGCTTTACTCAAATTAGTTCTCACCTGATAAGATCCAATTCT

Coding sequence (CDS)

TTGGTCTCTCATTTCCTCGCAGCAATTTCCTCGTACCCTCCGTTCTCCTTCCTGTTCCAGCACATGTCCCCATTCAAAACTGCGCAAATCCACCTTGTGAGTTCTCATCGTGAGGTTTATGAACCCTGTGATGATTCATTTGCTTTGGTTGATGCACTTTTAGCTGACCGAGTTAACTTGTTAAATCATCACCCAACATTGTGCTTAGAAGTAGGCTGTGGAAGTGGGTATGTCATAACATCTCTTGCTCTTATGTTGGGAAAGGAAGCCTCTGGCACTCACTATATCGCTACAGACATCAATCCTCATGCAACGAGAGTCACTCAGGAGACACTAGAAGCACATGGGGTTCATGCGGAGTTGGTATCCACTGACATAGCATATGGTTTGGAGAAACGACTGGCTTCATCTGTTGATGTGTTGATTGTGAATCCACCCTATGTTCCAACCCCTGAGGAGGAAGTGGGTCTTGAAGGAATTGCCTCTGCTTGGGCAGGAGGGGAAAATGGCCGCAGTGTTATCGATAGAATGTTGCCAGTTGCTGATGCTCTTTTGTCTGACAAAGGCTGGTTGTACATGGTTTTTCTTGAAGCAAACAATCCCTCCCAGATATGCCTTCAAATGAGAGAAAAGGGGTATGCTAGTCGAATCGTTGTGCAGAGATCAACTGAAGAAGAGAGTCTTCATGTGATCAAGTTTTGGAAGAATGCTGATCTTCAAGTTCATGGAAAAGGACTTGAGATTCATCAGGACATCCTCTGTTGTTACAAGGAACTAGCCCATTTTTCAAAATTTGTGCATTCTACAGGTGCGTATTGTTCGATTTTGTATATCAAATACATTTGTTTTGACCCTGTTACTGCTTAG

Protein sequence

LVSHFLAAISSYPPFSFLFQHMSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVLIVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKGLEIHQDILCCYKELAHFSKFVHSTGAYCSILYIKYICFDPVTA

Homology

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BLAST of Carg23052 vs. NCBI nr
Match: KAG7022515.1 (HemK methyltransferase family member 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 588.2 bits (1515), Expect = 3.9e-164
Identity = 288/288 (100.00%), Postives = 288/288 (100.00%), Query Frame = 0


Query: 1   LVSHFLAAISSYPPFSFLFQHMSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNL 60
           LVSHFLAAISSYPPFSFLFQHMSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNL
Sbjct: 1   LVSHFLAAISSYPPFSFLFQHMSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNL 60

Query: 61  LNHHPTLCLEVGCGSGYVITSLALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAE 120
           LNHHPTLCLEVGCGSGYVITSLALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAE
Sbjct: 61  LNHHPTLCLEVGCGSGYVITSLALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAE 120

Query: 121 LVSTDIAYGLEKRLASSVDVLIVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPV 180
           LVSTDIAYGLEKRLASSVDVLIVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPV
Sbjct: 121 LVSTDIAYGLEKRLASSVDVLIVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPV 180

Query: 181 ADALLSDKGWLYMVFLEANNPSQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQ 240
           ADALLSDKGWLYMVFLEANNPSQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQ
Sbjct: 181 ADALLSDKGWLYMVFLEANNPSQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQ 240

Query: 241 VHGKGLEIHQDILCCYKELAHFSKFVHSTGAYCSILYIKYICFDPVTA 289
           VHGKGLEIHQDILCCYKELAHFSKFVHSTGAYCSILYIKYICFDPVTA
Sbjct: 241 VHGKGLEIHQDILCCYKELAHFSKFVHSTGAYCSILYIKYICFDPVTA 288

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BLAST of Carg23052 vs. NCBI nr
Match: XP_022927839.1 (hemK methyltransferase family member 2-like [Cucurbita moschata] >XP_022927840.1 hemK methyltransferase family member 2-like [Cucurbita moschata])

HSP 1 Score: 454.1 bits (1167), Expect = 8.8e-124
Identity = 223/223 (100.00%), Postives = 223/223 (100.00%), Query Frame = 0


Query: 22  MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 81
           MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS
Sbjct: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60

Query: 82  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVL 141
           LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVL
Sbjct: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVL 120

Query: 142 IVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 201
           IVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP
Sbjct: 121 IVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180

Query: 202 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK 245
           SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK
Sbjct: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK 223

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BLAST of Carg23052 vs. NCBI nr
Match: XP_023530050.1 (hemK methyltransferase family member 2-like [Cucurbita pepo subsp. pepo] >XP_023530051.1 hemK methyltransferase family member 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 452.6 bits (1163), Expect = 2.5e-123
Identity = 221/223 (99.10%), Postives = 223/223 (100.00%), Query Frame = 0


Query: 22  MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 81
           MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS
Sbjct: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60

Query: 82  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVL 141
           LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAEL+STDIAYGLEKRLASSVDVL
Sbjct: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120

Query: 142 IVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 201
           IVNPPYVPTPE+EVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP
Sbjct: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180

Query: 202 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK 245
           SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK
Sbjct: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK 223

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BLAST of Carg23052 vs. NCBI nr
Match: XP_022988929.1 (hemK methyltransferase family member 2-like [Cucurbita maxima] >XP_022988930.1 hemK methyltransferase family member 2-like [Cucurbita maxima])

HSP 1 Score: 450.3 bits (1157), Expect = 1.3e-122
Identity = 220/223 (98.65%), Postives = 222/223 (99.55%), Query Frame = 0


Query: 22  MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 81
           MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS
Sbjct: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60

Query: 82  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVL 141
           LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAEL+STDIAYGLEKRLASSVDVL
Sbjct: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120

Query: 142 IVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 201
           IVNPPYVPTPE+EVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP
Sbjct: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180

Query: 202 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK 245
           SQICLQMREKGYASRIVVQR TEEESLHVIKFWKNADLQVHGK
Sbjct: 181 SQICLQMREKGYASRIVVQRLTEEESLHVIKFWKNADLQVHGK 223

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BLAST of Carg23052 vs. NCBI nr
Match: KAG6571894.1 (putative protein S-acyltransferase 23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 429.5 bits (1103), Expect = 2.3e-116
Identity = 213/239 (89.12%), Postives = 222/239 (92.89%), Query Frame = 0


Query: 6   LAAISSYPPFSFLFQHMSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHP 65
           L  +SS+    FL QHMS F+TAQIHLVSSHREVYEPCDDSFALVDALLA+RVNLLNHHP
Sbjct: 551 LFPVSSHLRSPFLVQHMSSFRTAQIHLVSSHREVYEPCDDSFALVDALLAERVNLLNHHP 610

Query: 66  TLCLEVGCGSGYVITSLALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTD 125
           TLCLEVGCGSGYVITSLALMLGKEASG HYIATDINPHA RVTQETLEAHGVHAELVSTD
Sbjct: 611 TLCLEVGCGSGYVITSLALMLGKEASGAHYIATDINPHAIRVTQETLEAHGVHAELVSTD 670

Query: 126 IAYGLEKRLASSVDVLIVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALL 185
           IA GLEKRLA SVDVL+VNPPYVPTPE+EVG +GIASAWAGGENGRSVIDR+LPVAD LL
Sbjct: 671 IASGLEKRLAGSVDVLVVNPPYVPTPEDEVGRDGIASAWAGGENGRSVIDRILPVADVLL 730

Query: 186 SDKGWLYMVFLEANNPSQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK 245
           SDKGWLYMV L ANNPSQICLQMREKGYASRIVVQRSTEEESLHVIKFWK+ADLQV GK
Sbjct: 731 SDKGWLYMVTLTANNPSQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGK 789

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BLAST of Carg23052 vs. ExPASy Swiss-Prot
Match: Q6SKR2 (Methyltransferase N6AMT1 OS=Mus musculus OX=10090 GN=N6amt1 PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 6.0e-43
Identity = 93/200 (46.50%), Postives = 122/200 (61.00%), Query Frame = 0


Query: 37  REVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALMLGKEASGTHYI 96
           R+VYEP +D+F L+DAL A    L      +CLEVG GSG V   LA M+G  A    Y+
Sbjct: 20  RDVYEPAEDTFLLLDALEAAAAELAG--VEICLEVGAGSGVVSAFLASMIGPRAL---YM 79

Query: 97  ATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVLIVNPPYVPTPEEEVG 156
            TDINP A   T ET   + VH + V TD+ +GL  RL   VD+L+ NPPYV TP EEVG
Sbjct: 80  CTDINPEAAACTLETARCNRVHVQPVITDLVHGLLPRLKGKVDLLVFNPPYVVTPPEEVG 139

Query: 157 LEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQICLQMREKGYASR 216
             GI +AWAGG NGR V+DR  P+A  LLS +G  Y+V ++ NNP +I   M+ +G    
Sbjct: 140 SRGIEAAWAGGRNGREVMDRFFPLAPELLSPRGLFYLVTVKENNPEEIFKTMKTRGLQGT 199

Query: 217 IVVQRSTEEESLHVIKFWKN 237
             + R   +E+L V++F K+
Sbjct: 200 TALCRQAGQEALSVLRFSKS 214

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BLAST of Carg23052 vs. ExPASy Swiss-Prot
Match: Q9Y5N5 (Methyltransferase N6AMT1 OS=Homo sapiens OX=9606 GN=N6AMT1 PE=1 SV=4)

HSP 1 Score: 173.7 bits (439), Expect = 3.0e-42
Identity = 92/199 (46.23%), Postives = 120/199 (60.30%), Query Frame = 0


Query: 38  EVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALMLGKEASGTHYIA 97
           +VYEP +D+F L+DAL A    L      +CLEVG GSG V   LA M+G +A    Y+ 
Sbjct: 21  DVYEPAEDTFLLLDALEAAAAELAG--VEICLEVGSGSGVVSAFLASMIGPQAL---YMC 80

Query: 98  TDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVLIVNPPYVPTPEEEVGL 157
           TDINP A   T ET   + VH + V TD+  GL  RL   VD+L+ NPPYV TP +EVG 
Sbjct: 81  TDINPEAAACTLETARCNKVHIQPVITDLVKGLLPRLTEKVDLLVFNPPYVVTPPQEVGS 140

Query: 158 EGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQICLQMREKGYASRI 217
            GI +AWAGG NGR V+DR  P+   LLS +G  Y+V ++ NNP +I   M+ KG     
Sbjct: 141 HGIEAAWAGGRNGREVMDRFFPLVPDLLSPRGLFYLVTIKENNPEEILKIMKTKGLQGTT 200

Query: 218 VVQRSTEEESLHVIKFWKN 237
            + R   +E+L V+KF K+
Sbjct: 201 ALSRQAGQETLSVLKFTKS 214

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BLAST of Carg23052 vs. ExPASy Swiss-Prot
Match: Q9UT94 (eRF1 methyltransferase catalytic subunit mtq2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mtq2 PE=3 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.6e-22
Identity = 75/221 (33.94%), Postives = 114/221 (51.58%), Query Frame = 0


Query: 27  TAQIHLVSSHREVYEPCDDSFALVDALLADRVNL---LNHHPTLCLEVGCGSGYVITSLA 86
           T+Q+ L    ++VYEP +D+FAL+DAL  D   L         L  E+GCGSG   + L 
Sbjct: 7   TSQLRL-KEFQDVYEPAEDTFALLDALEKDAKKLRQMAEMKNLLTAEIGCGSGCASSFLK 66

Query: 87  LMLGKEASGTHYIATDINPHATRVTQET-LEAHGVHAE------LVSTDIAYGLEKRLAS 146
             + K     H++ +DI+  A R ++ T L    ++ +       V T    G+  RL +
Sbjct: 67  SGILKNKPIVHFM-SDISNSACRASKITALNNRELYKDDNGLFITVQTSFLDGI--RLGN 126

Query: 147 SVDVLIVNPPYVPTPEEEVGLEG--IASAWAGGENGRSVIDRMLPVADALLSDKGWLYMV 206
            VD+LI NPPYVPT  EE+  E   IASAWAGG +G  V   +L     +LS  G  YMV
Sbjct: 127 GVDILIFNPPYVPTEFEEIPSEAATIASAWAGGTDGMDVTSTLLNQLKDILSQDGVFYMV 186

Query: 207 FLEANNPSQICLQMREKGYASRIVVQRSTEEESLHVIKFWK 236
            +  N    IC  +++ G+     ++R    E+L +++ ++
Sbjct: 187 AVARNKLHSICEILQKDGFIVNETLKRKAGRETLSILRIYR 223

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BLAST of Carg23052 vs. ExPASy Swiss-Prot
Match: Q58338 (Putative protein N5-glutamine methyltransferase MJ0928 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0928 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 5.3e-15
Identity = 54/200 (27.00%), Postives = 94/200 (47.00%), Query Frame = 0


Query: 36  HREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALMLGKEASGTHY 95
           H EVYEP +DS  L+        NL++      LE+G G+G +  + A    K+  G   
Sbjct: 13  HPEVYEPAEDSILLLK-------NLVDVKNKDVLEIGVGTGLISIACAKKGAKKIVG--- 72

Query: 96  IATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVLIVNPPYVPTPEEEV 155
              DINP+A ++ +E  + + V+     +D    L + +    DV++ NPPY+PT E+E 
Sbjct: 73  --VDINPYAVKLAKENAKLNNVNISFFESD----LFENVTGKFDVILFNPPYLPTSEDEK 132

Query: 156 GLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQICLQMREKGYAS 215
               +  A+ GG++GR ++DR +      L   G + ++        +   +++  G+  
Sbjct: 133 IDSYLNFAFDGGKDGREILDRFIYELPNYLKKGGVVQILQSSLTGEKETINKLKPLGFKV 192

Query: 216 RIVVQRSTEEESLHVIKFWK 236
            I  +     E L VI  W+
Sbjct: 193 EISARLKVPFEELMVINAWR 196

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BLAST of Carg23052 vs. ExPASy Swiss-Prot
Match: Q03920 (eRF1 methyltransferase catalytic subunit MTQ2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MTQ2 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.6e-14
Identity = 53/180 (29.44%), Postives = 87/180 (48.33%), Query Frame = 0


Query: 38  EVYEPCDDSFALVDALLADRVNL---LNHHPTLCLEVGCGSGYVITSLALMLGKEASGTH 97
           +VYEP +DSF ++D L  +   L     +   +  E+G GSG V T L          + 
Sbjct: 13  KVYEPAEDSFLILDCLEKEHDFLKQKFGNRLAIVCEIGSGSGIVTTFLMQNKIIPQENSI 72

Query: 98  YIATDINPHATRVTQETLEAHGVHA---ELVSTDIAYGLEKRLASSVDVLIVNPPYVPTP 157
           ++A DINP A   T +T + +   +   E++  D+   +     + VDVLI NPPYVP  
Sbjct: 73  HLAVDINPWALEATLDTAKLNSCKSSFLEVIQADLNSSIRN---NQVDVLIFNPPYVPAE 132

Query: 158 -------EEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQI 205
                    E   + +  A  GG++G ++ D++L   + +LS  G  Y++F   N P ++
Sbjct: 133 CVPDVPGSREEADQWLDLALLGGKDGMAITDKLLRQLEQILSPDGVAYILFCARNKPKEV 189

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BLAST of Carg23052 vs. ExPASy TrEMBL
Match: A0A6J1EJ44 (hemK methyltransferase family member 2-like OS=Cucurbita moschata OX=3662 GN=LOC111434608 PE=3 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 4.2e-124
Identity = 223/223 (100.00%), Postives = 223/223 (100.00%), Query Frame = 0


Query: 22  MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 81
           MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS
Sbjct: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60

Query: 82  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVL 141
           LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVL
Sbjct: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVL 120

Query: 142 IVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 201
           IVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP
Sbjct: 121 IVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180

Query: 202 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK 245
           SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK
Sbjct: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK 223

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BLAST of Carg23052 vs. ExPASy TrEMBL
Match: A0A6J1JKY8 (hemK methyltransferase family member 2-like OS=Cucurbita maxima OX=3661 GN=LOC111486137 PE=3 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 6.1e-123
Identity = 220/223 (98.65%), Postives = 222/223 (99.55%), Query Frame = 0


Query: 22  MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 81
           MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS
Sbjct: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60

Query: 82  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVL 141
           LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAEL+STDIAYGLEKRLASSVDVL
Sbjct: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120

Query: 142 IVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 201
           IVNPPYVPTPE+EVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP
Sbjct: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180

Query: 202 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK 245
           SQICLQMREKGYASRIVVQR TEEESLHVIKFWKNADLQVHGK
Sbjct: 181 SQICLQMREKGYASRIVVQRLTEEESLHVIKFWKNADLQVHGK 223

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BLAST of Carg23052 vs. ExPASy TrEMBL
Match: A0A6J1GME1 (hemK methyltransferase family member 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455633 PE=3 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 2.0e-113
Identity = 205/223 (91.93%), Postives = 212/223 (95.07%), Query Frame = 0


Query: 22  MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 81
           MS F+TAQIHLVSSHREVYEPCDDSFALVDALLA+RVNLLNHHPTLC EVGCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLAERVNLLNHHPTLCFEVGCGSGYVITS 60

Query: 82  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVL 141
           LALMLGKEASG HYIATDINPHA RVTQETLEAHGVHAELVSTDIA GLEKRLA SVDVL
Sbjct: 61  LALMLGKEASGAHYIATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEKRLAGSVDVL 120

Query: 142 IVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 201
           +VNPPYVPTPE+EVG +GIASAWAGGENGRSVIDR+LPVAD LLSDKGWLYMV L ANNP
Sbjct: 121 VVNPPYVPTPEDEVGRDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 202 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK 245
           SQICLQMREKGYASRIVVQRSTEEESLHVIKFWK+ADLQV GK
Sbjct: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGK 223

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BLAST of Carg23052 vs. ExPASy TrEMBL
Match: A0A6J1I5Y4 (hemK methyltransferase family member 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470989 PE=3 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 9.8e-113
Identity = 205/223 (91.93%), Postives = 212/223 (95.07%), Query Frame = 0


Query: 22  MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 81
           MS F+TAQIHLVSSHREVYEPCDDSFALVDALLA+RVNLLNHHPTLCLEVGCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLAERVNLLNHHPTLCLEVGCGSGYVITS 60

Query: 82  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVL 141
           LALMLGKEASG HYIATDINPHA RVTQETLEAHGVHAELVSTDIA GLEKRLA SVDVL
Sbjct: 61  LALMLGKEASGAHYIATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEKRLAGSVDVL 120

Query: 142 IVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 201
           +VNPPYVPTPE+EVG +GIASAWAGGENGRSVIDR+L VAD LLSDKGWLYMV L ANNP
Sbjct: 121 VVNPPYVPTPEDEVGRDGIASAWAGGENGRSVIDRILLVADVLLSDKGWLYMVTLTANNP 180

Query: 202 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK 245
           SQICLQMREKGYASRIVVQRSTEEESLHVIKFWK+ADLQV GK
Sbjct: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGK 223

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BLAST of Carg23052 vs. ExPASy TrEMBL
Match: A0A6J1DPY1 (hemK methyltransferase family member 2 OS=Momordica charantia OX=3673 GN=LOC111022463 PE=3 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 1.7e-112
Identity = 203/223 (91.03%), Postives = 211/223 (94.62%), Query Frame = 0


Query: 22  MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 81
           MS F+TAQIHLVSSHREVYEPCDDSFALVDALLADR NLLNH+PTLCLEVGCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLADRANLLNHNPTLCLEVGCGSGYVITS 60

Query: 82  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVL 141
           LALMLGKEASGTHYIATDINPHA RVTQETLEAHGVHAELVSTDIA G+EKRLA  VDVL
Sbjct: 61  LALMLGKEASGTHYIATDINPHALRVTQETLEAHGVHAELVSTDIASGMEKRLAGIVDVL 120

Query: 142 IVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 201
           +VNPPYVPTPE+EVG EGIASAWAGGENGRSVIDR+LPVAD LLSDKGW YMV L ANNP
Sbjct: 121 VVNPPYVPTPEDEVGREGIASAWAGGENGRSVIDRILPVADVLLSDKGWFYMVTLTANNP 180

Query: 202 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK 245
           SQICLQMREKGYASRI+VQRSTEEESLHVIKFWK+ADLQV GK
Sbjct: 181 SQICLQMREKGYASRIIVQRSTEEESLHVIKFWKDADLQVDGK 223

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BLAST of Carg23052 vs. TAIR 10
Match: AT3G13440.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 331.3 bits (848), Expect = 8.0e-91
Identity = 158/231 (68.40%), Postives = 191/231 (82.68%), Query Frame = 0


Query: 14  PFSFLFQHMSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGC 73
           P S +   M P + A I LVSSHREVYEPCDDSFALVDALLADR NL+ H+P +C+E+GC
Sbjct: 25  PHSQVIYKMPP-RIAAIRLVSSHREVYEPCDDSFALVDALLADRTNLIEHNPKICMEIGC 84

Query: 74  GSGYVITSLALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKR 133
           GSGYVITSL L+L  E  G HY+A D NP ATRVT+ETLEAHGV+A+++  D+A GLEKR
Sbjct: 85  GSGYVITSLILLLQNEVPGVHYLAIDTNPIATRVTKETLEAHGVNADVICADLATGLEKR 144

Query: 134 LASSVDVLIVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYM 193
           LA SVDV++VNPPYVPTPE EVG+EGIASAWAGGENGR+VID++LPV D LLS+KGW Y+
Sbjct: 145 LAGSVDVIVVNPPYVPTPEYEVGMEGIASAWAGGENGRTVIDKILPVVDLLLSEKGWFYL 204

Query: 194 VFLEANNPSQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK 245
           V L +N P++ICL MR+KGYASRIVVQRSTEEE+L ++KFW++ D +   K
Sbjct: 205 VTLTSNFPAEICLMMRKKGYASRIVVQRSTEEENLVILKFWRDKDEESQDK 254

The following BLAST results are available for this feature:

BLAST of Carg23052 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7022515.1	3.9e-164	100.00	HemK methyltransferase family member 2, partial [Cucurbita argyrosperma subsp. a...	[more]
XP_022927839.1	8.8e-124	100.00	hemK methyltransferase family member 2-like [Cucurbita moschata] >XP_022927840.1...	[more]
XP_023530050.1	2.5e-123	99.10	hemK methyltransferase family member 2-like [Cucurbita pepo subsp. pepo] >XP_023...	[more]
XP_022988929.1	1.3e-122	98.65	hemK methyltransferase family member 2-like [Cucurbita maxima] >XP_022988930.1 h...	[more]
KAG6571894.1	2.3e-116	89.12	putative protein S-acyltransferase 23, partial [Cucurbita argyrosperma subsp. so...	[more]

BLAST of Carg23052 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6SKR2	6.0e-43	46.50	Methyltransferase N6AMT1 OS=Mus musculus OX=10090 GN=N6amt1 PE=1 SV=1	[more]
Q9Y5N5	3.0e-42	46.23	Methyltransferase N6AMT1 OS=Homo sapiens OX=9606 GN=N6AMT1 PE=1 SV=4	[more]
Q9UT94	2.6e-22	33.94	eRF1 methyltransferase catalytic subunit mtq2 OS=Schizosaccharomyces pombe (stra...	[more]
Q58338	5.3e-15	27.00	Putative protein N5-glutamine methyltransferase MJ0928 OS=Methanocaldococcus jan...	[more]
Q03920	2.6e-14	29.44	eRF1 methyltransferase catalytic subunit MTQ2 OS=Saccharomyces cerevisiae (strai...	[more]

BLAST of Carg23052 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EJ44	4.2e-124	100.00	hemK methyltransferase family member 2-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1JKY8	6.1e-123	98.65	hemK methyltransferase family member 2-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1GME1	2.0e-113	91.93	hemK methyltransferase family member 2-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1I5Y4	9.8e-113	91.93	hemK methyltransferase family member 2-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1DPY1	1.7e-112	91.03	hemK methyltransferase family member 2 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Carg23052 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13440.1	8.0e-91	68.40	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 37..242 e-value: 5.6E-42 score: 145.3
None	No IPR available	PANTHER	PTHR45875	METHYLTRANSFERASE N6AMT1	coord: 25..238
None	No IPR available	PANTHER	PTHR45875:SF5	BNAC01G37640D PROTEIN	coord: 25..238
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 69..198 e-value: 2.3351E-7 score: 46.2691
IPR004557	Eukaryotic/archaeal PrmC-related	TIGRFAM	TIGR00537	TIGR00537	coord: 39..234 e-value: 9.5E-27 score: 91.8
IPR007848	Methyltransferase small domain	PFAM	PF05175	MTS	coord: 64..150 e-value: 1.5E-9 score: 37.7
IPR002052	DNA methylase, N-6 adenine-specific, conserved site	PROSITE	PS00092	N6_MTASE	coord: 141..147
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 44..220

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23052-RA	Carg23052-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006479	protein methylation
biological_process	GO:0032259	methylation
cellular_component	GO:0035657	eRF1 methyltransferase complex
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008276	protein methyltransferase activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity