Carg22217 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg22217
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	WRKY protein
Location	Carg_Chr16: 3310847 .. 3311706 (-)
RNA-Seq Expression	Carg22217
Synteny	Carg22217

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGATTGGGATTTGCAAGCAATTGTAAAAGGATGCAACGGATCAATTACTCCTTCAAGAACAGCAACGCTCATGGAAGATCCAAATTTGGTCTCCTTTTTGATCGAAAAAGATGAACTTTTAAGTTCTTGTTCAGTGTATGATGAGTTTGAAGGGTTGTTTGGAAGCTCCGATGACTCTGCCTCTGCCTCTGCCTCTGCCGCCGCCATTTCTGGTCTTCTCAGAGAGCTGAAGGAACCGGAAAAGCTTCATCGGAAGAGCCAAATCGCTGCAACTAAACAGAAACAGAGGTGGGTACTTCGAATTTAGATTCAAAATTTTATCTTTTGAAGGATTTGATTGAGTTTTGATTTGGTATCAGCAAGAAAAGTCGGGAAAACAGAGTAGTGAAAGAGGTGAAAGCAGAGGGGGTGTGTTCGGATTCATGGGGATGGCGGAAATATGGACAGAAACCGATTAAAGGGTCGCCATATCCGAGAAGCTATTATCGATGTAGCTCCTCCAAAGGATGCTCTGCTAGAAAGCAAGTTGAACGGAGCCTCTCCGATCCCGGCGCCTTTGTCGTAACCTACACGGCTGAGCACAACCACGCTGAGCCTACTCGCCGTAACGCGCTTGCCGGAACCACCAGGAAGAAGTTTCCGGCGCCGGAAAATCCGAGGTTGGACGTGATTCTGTCGCCTAGTAATTCTATGTCCGAGGCGTCCGTTGAAGAAGAACAGCCGATGGAGGGAGTTGGCAGAGGGGGGAGTTATTATGGATTTGCCATTCGAATTCTTTTCTGGTTTGGAGGATTTTCTTTTCGAATGACATTTTTTTTTTTTTTTTTTTAAGAGAATGTGAATTGGCAATTGTAA

mRNA sequence

ATGGAGGATTGGGATTTGCAAGCAATTGTAAAAGGATGCAACGGATCAATTACTCCTTCAAGAACAGCAACGCTCATGGAAGATCCAAATTTGGTCTCCTTTTTGATCGAAAAAGATGAACTTTTAAGTTCTTGTTCAGTGTATGATGAGTTTGAAGGGTTGTTTGGAAGCTCCGATGACTCTGCCTCTGCCTCTGCCTCTGCCGCCGCCATTTCTGGTCTTCTCAGAGAGCTGAAGGAACCGGAAAAGCTTCATCGGAAGAGCCAAATCGCTGCAACTAAACAGAAACAGAGCAAGAAAAGTCGGGAAAACAGAGTAGTGAAAGAGGTGAAAGCAGAGGGGGTGTGTTCGGATTCATGGGGATGGCGGAAATATGGACAGAAACCGATTAAAGGGTCGCCATATCCGAGAAGCTATTATCGATGTAGCTCCTCCAAAGGATGCTCTGCTAGAAAGCAAGTTGAACGGAGCCTCTCCGATCCCGGCGCCTTTGTCGTAACCTACACGGCTGAGCACAACCACGCTGAGCCTACTCGCCGTAACGCGCTTGCCGGAACCACCAGGAAGAAGTTTCCGGCGCCGGAAAATCCGAGGTTGGACGTGATTCTGTCGCCTAGTAATTCTATGTCCGAGGCGTCCGTTGAAGAAGAACAGCCGATGGAGGGAGTTGGCAGAGGGGGGAAGAATGTGAATTGGCAATTGTAA

Coding sequence (CDS)

ATGGAGGATTGGGATTTGCAAGCAATTGTAAAAGGATGCAACGGATCAATTACTCCTTCAAGAACAGCAACGCTCATGGAAGATCCAAATTTGGTCTCCTTTTTGATCGAAAAAGATGAACTTTTAAGTTCTTGTTCAGTGTATGATGAGTTTGAAGGGTTGTTTGGAAGCTCCGATGACTCTGCCTCTGCCTCTGCCTCTGCCGCCGCCATTTCTGGTCTTCTCAGAGAGCTGAAGGAACCGGAAAAGCTTCATCGGAAGAGCCAAATCGCTGCAACTAAACAGAAACAGAGCAAGAAAAGTCGGGAAAACAGAGTAGTGAAAGAGGTGAAAGCAGAGGGGGTGTGTTCGGATTCATGGGGATGGCGGAAATATGGACAGAAACCGATTAAAGGGTCGCCATATCCGAGAAGCTATTATCGATGTAGCTCCTCCAAAGGATGCTCTGCTAGAAAGCAAGTTGAACGGAGCCTCTCCGATCCCGGCGCCTTTGTCGTAACCTACACGGCTGAGCACAACCACGCTGAGCCTACTCGCCGTAACGCGCTTGCCGGAACCACCAGGAAGAAGTTTCCGGCGCCGGAAAATCCGAGGTTGGACGTGATTCTGTCGCCTAGTAATTCTATGTCCGAGGCGTCCGTTGAAGAAGAACAGCCGATGGAGGGAGTTGGCAGAGGGGGGAAGAATGTGAATTGGCAATTGTAA

Protein sequence

MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDDSASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPTRRNALAGTTRKKFPAPENPRLDVILSPSNSMSEASVEEEQPMEGVGRGGKNVNWQL

Homology

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BLAST of Carg22217 vs. NCBI nr
Match: KAG7015174.1 (WRKY transcription factor 22, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 459.9 bits (1182), Expect = 1.3e-125
Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 0


Query: 1   MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60
           MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD
Sbjct: 1   MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60

Query: 61  SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW 120
           SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW
Sbjct: 61  SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW 120

Query: 121 GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPTRR 180
           GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPTRR
Sbjct: 121 GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPTRR 180

Query: 181 NALAGTTRKKFPAPENPRLDVILSPSNSMSEASVEEEQPMEGVGRGGKNVNWQL 235
           NALAGTTRKKFPAPENPRLDVILSPSNSMSEASVEEEQPMEGVGRGGKNVNWQL
Sbjct: 181 NALAGTTRKKFPAPENPRLDVILSPSNSMSEASVEEEQPMEGVGRGGKNVNWQL 234

[Close]

BLAST of Carg22217 vs. NCBI nr
Match: KAG6577177.1 (WRKY transcription factor 22, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 436.8 bits (1122), Expect = 1.2e-118
Identity = 224/227 (98.68%), Postives = 224/227 (98.68%), Query Frame = 0


Query: 1   MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60
           MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD
Sbjct: 1   MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60

Query: 61  SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW 120
           SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW
Sbjct: 61  SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW 120

Query: 121 GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPTRR 180
           GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPTRR
Sbjct: 121 GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPTRR 180

Query: 181 NALAGTTRKKFPAPENPRLDVILSPSNSMSEASVEEEQPMEGVGRGG 228
           NALAGTTRKKFPAPENPRLDVILSPSNSMS ASVEEEQPMEGV  GG
Sbjct: 181 NALAGTTRKKFPAPENPRLDVILSPSNSMSVASVEEEQPMEGVAEGG 227

[Close]

BLAST of Carg22217 vs. NCBI nr
Match: XP_022931440.1 (probable WRKY transcription factor 29 [Cucurbita moschata])

HSP 1 Score: 435.3 bits (1118), Expect = 3.4e-118
Identity = 223/227 (98.24%), Postives = 224/227 (98.68%), Query Frame = 0


Query: 1   MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60
           MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD
Sbjct: 1   MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60

Query: 61  SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW 120
           SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW
Sbjct: 61  SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW 120

Query: 121 GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPTRR 180
           GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTY+AEHNHAEPTRR
Sbjct: 121 GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYSAEHNHAEPTRR 180

Query: 181 NALAGTTRKKFPAPENPRLDVILSPSNSMSEASVEEEQPMEGVGRGG 228
           NALAGTTRKKFPAPENPRLDVILSPSNSMS ASVEEEQPMEGV  GG
Sbjct: 181 NALAGTTRKKFPAPENPRLDVILSPSNSMSVASVEEEQPMEGVAEGG 227

[Close]

BLAST of Carg22217 vs. NCBI nr
Match: XP_022985418.1 (probable WRKY transcription factor 29 [Cucurbita maxima])

HSP 1 Score: 407.1 bits (1045), Expect = 1.0e-109
Identity = 211/223 (94.62%), Postives = 213/223 (95.52%), Query Frame = 0


Query: 1   MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60
           MEDWDLQAIVKGCNGSIT SRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD
Sbjct: 1   MEDWDLQAIVKGCNGSITSSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60

Query: 61  SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW 120
           SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSR+NRVVKEVKAEGVCSDSW
Sbjct: 61  SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRQNRVVKEVKAEGVCSDSW 120

Query: 121 GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPTRR 180
           GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFV+TYTAEHNHAEPTRR
Sbjct: 121 GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVITYTAEHNHAEPTRR 180

Query: 181 NALAGTTRKKFPAPENPRLDVILSPSNSMSEASVEEEQPMEGV 224
           NALAGTTRKKFPAPENPRLD        MS ASVEEEQPMEGV
Sbjct: 181 NALAGTTRKKFPAPENPRLD--------MSVASVEEEQPMEGV 215

[Close]

BLAST of Carg22217 vs. NCBI nr
Match: XP_023552880.1 (probable WRKY transcription factor 29 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 399.1 bits (1024), Expect = 2.7e-107
Identity = 210/229 (91.70%), Postives = 212/229 (92.58%), Query Frame = 0


Query: 1   MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60
           MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDE+LSSCSVYDEFEGLFGSSDD
Sbjct: 104 MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDEILSSCSVYDEFEGLFGSSDD 163

Query: 61  --SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSD 120
             SASASASAAAISGLLRELKEPEKLHRKS IAATKQKQSKKSR+NRVVKEVKAEGVCSD
Sbjct: 164 SASASASASAAAISGLLRELKEPEKLHRKSHIAATKQKQSKKSRQNRVVKEVKAEGVCSD 223

Query: 121 SWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPT 180
           SWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPT
Sbjct: 224 SWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPT 283

Query: 181 RRNALAGTTRKKFPAPENPRLDVILSPSNSMSEASVEEEQPMEGVGRGG 228
           RRNALAGTTRKKFPAPEN  LD         S ASVEEEQPMEGV  GG
Sbjct: 284 RRNALAGTTRKKFPAPENTSLD--------RSVASVEEEQPMEGVAEGG 324

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BLAST of Carg22217 vs. ExPASy Swiss-Prot
Match: Q9SUS1 (Probable WRKY transcription factor 29 OS=Arabidopsis thaliana OX=3702 GN=WRKY29 PE=2 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.9e-34
Identity = 102/246 (41.46%), Postives = 137/246 (55.69%), Query Frame = 0


Query: 1   MEDWDLQAIVKGCNGS----------------ITPSRTATLMEDPNLVSFLIEKDELLSS 60
           M++ DL+AIV+G +GS                  P  T++  E     S L E  EL   
Sbjct: 1   MDEGDLEAIVRGYSGSGDAFSGESSGTFSPSFCLPMETSSFYEPEMETSGLDELGEL--- 60

Query: 61  CSVYDEFEGLFGSSDDSASASASAAAISGLLRELKEPEK----LHRKS-----QIAATKQ 120
              Y  F     S+    ++S S    S   R+ K+       L   S     +I+ +K 
Sbjct: 61  ---YKPFYPF--STQTILTSSVSLPEDSKPFRDDKKQRSHGCLLSNGSRADHIRISESKS 120

Query: 121 KQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVE 180
           K+SKK+++ RVV++VK E + SD+W WRKYGQKPIKGSPYPRSYYRCSSSKGC ARKQVE
Sbjct: 121 KKSKKNQQKRVVEQVKEENLLSDAWAWRKYGQKPIKGSPYPRSYYRCSSSKGCLARKQVE 180

Query: 181 RSLSDPGAFVVTYTAEHNHAEPTRRNALAGTTRKKFPAP-----ENPRLDVILSPSNSMS 217
           R+  +P  F +TYT EHNH  PTRRN+LAG+TR K   P     +    +V+ SP+++  
Sbjct: 181 RNPQNPEKFTITYTNEHNHELPTRRNSLAGSTRAKTSQPKPTLTKKSEKEVVSSPTSNPM 238

[Close]

BLAST of Carg22217 vs. ExPASy Swiss-Prot
Match: O04609 (WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.7e-32
Identity = 86/200 (43.00%), Postives = 115/200 (57.50%), Query Frame = 0


Query: 2   EDWDLQAIVKGCNGSITPSRTAT-----LMEDPNLVSFLIEKDELLSSCSVYDEFEGLFG 61
           +DWDL A+V+GC+ +++ S T T     +    N +  +  +   +S   + D +     
Sbjct: 3   DDWDLHAVVRGCS-AVSSSATTTVYSPGVSSHTNPIFTVGRQSNAVSFGEIRDLYTPFTQ 62

Query: 62  SSDDSASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSR------ENRVVKEV 121
            S  S+ +  +        +  K P  L   S    +K   S  SR      +++ V  V
Sbjct: 63  ESVVSSFSCINYPEEPRKPQNQKRPLSLSASSGSVTSKPSGSNTSRSKRRKIQHKKVCHV 122

Query: 122 KAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTA 181
            AE + SD W WRKYGQKPIKGSPYPR YYRCS+SKGC ARKQVER+ SDP  F+VTYTA
Sbjct: 123 AAEALNSDVWAWRKYGQKPIKGSPYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTA 182

Query: 182 EHNHAEPTRRNALAGTTRKK 191
           EHNH  PT RN+LAG+TR+K
Sbjct: 183 EHNHPAPTHRNSLAGSTRQK 201

[Close]

BLAST of Carg22217 vs. ExPASy Swiss-Prot
Match: Q9FLX8 (Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.6e-30
Identity = 69/123 (56.10%), Postives = 84/123 (68.29%), Query Frame = 0


Query: 95  QKQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQV 154
           + + +K+++ R +  V  E + SD W WRKYGQKPIKGSPYPR+YYRCSSSKGC ARKQV
Sbjct: 143 RSRKRKNQQKRTICHVTQENLSSDLWAWRKYGQKPIKGSPYPRNYYRCSSSKGCLARKQV 202

Query: 155 ERSLSDPGAFVVTYTAEHNHAEPTRRNALAGTTRKKFPAPENPRLDVILSPSNSMSEASV 214
           ERS  DP  F+VTYT EH H  PT RN+LAG+TR K   P NP    +  P  S    +V
Sbjct: 203 ERSNLDPNIFIVTYTGEHTHPRPTHRNSLAGSTRNK-SQPVNP----VPKPDTSPLSDTV 260

Query: 215 EEE 218
           +EE
Sbjct: 263 KEE 260

[Close]

BLAST of Carg22217 vs. ExPASy Swiss-Prot
Match: O64747 (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 4.5e-28
Identity = 74/166 (44.58%), Postives = 98/166 (59.04%), Query Frame = 0


Query: 59  DDSASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSD 118
           +++ ++S S   IS   R L    +  +  ++       +  SR +        E V SD
Sbjct: 164 NNNNTSSCSQVQISSSPRNLGIKRRKSQAKKVVCIPAPAAMNSRSS-------GEVVPSD 223

Query: 119 SWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPT 178
            W WRKYGQKPIKGSPYPR YYRCSSSKGCSARKQVERS +DP   V+TYT+EHNH  PT
Sbjct: 224 LWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPT 283

Query: 179 RRNALAGTTRKKFPAPENP-----RLDVILSPSNSMSEASVEEEQP 220
           +RNALAG+TR    +  NP           SPS+ + + +  +++P
Sbjct: 284 QRNALAGSTRSSSSSSLNPSSKSSTAAATTSPSSRVFQNNSSKDEP 322

[Close]

BLAST of Carg22217 vs. ExPASy Swiss-Prot
Match: Q9FL92 (Disease resistance protein RRS1B OS=Arabidopsis thaliana OX=3702 GN=RRS1B PE=1 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.4e-26
Identity = 73/144 (50.69%), Postives = 93/144 (64.58%), Query Frame = 0


Query: 77   ELKEPEKL--HRKSQIAATKQ-------KQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQ 136
            E K+ +KL  + +S + ATK+        + K ++E RVV  V      SD W WRKYGQ
Sbjct: 1131 ESKKTDKLVDNIQSSMIATKEIEITRSKSRRKNNKEKRVVCVVDRGSRSSDLWVWRKYGQ 1190

Query: 137  KPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPTRRNALAGTT 196
            KPIK SPYPRSYYRC+SSKGC ARKQVERS +DP   V+TY +EHNH  PT RN LAG+T
Sbjct: 1191 KPIKSSPYPRSYYRCASSKGCFARKQVERSRTDPNVSVITYISEHNHPFPTLRNTLAGST 1250

Query: 197  RKKFPAPENPRLDVILSPSNSMSE 212
            R    +  +   DV  S S+++S+
Sbjct: 1251 R----SSSSKCSDVTTSASSTVSQ 1270

[Close]

BLAST of Carg22217 vs. ExPASy TrEMBL
Match: A0A6J1EZF1 (probable WRKY transcription factor 29 OS=Cucurbita moschata OX=3662 GN=LOC111437621 PE=4 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.7e-118
Identity = 223/227 (98.24%), Postives = 224/227 (98.68%), Query Frame = 0


Query: 1   MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60
           MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD
Sbjct: 1   MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60

Query: 61  SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW 120
           SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW
Sbjct: 61  SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW 120

Query: 121 GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPTRR 180
           GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTY+AEHNHAEPTRR
Sbjct: 121 GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYSAEHNHAEPTRR 180

Query: 181 NALAGTTRKKFPAPENPRLDVILSPSNSMSEASVEEEQPMEGVGRGG 228
           NALAGTTRKKFPAPENPRLDVILSPSNSMS ASVEEEQPMEGV  GG
Sbjct: 181 NALAGTTRKKFPAPENPRLDVILSPSNSMSVASVEEEQPMEGVAEGG 227

[Close]

BLAST of Carg22217 vs. ExPASy TrEMBL
Match: A0A6J1JDJ7 (probable WRKY transcription factor 29 OS=Cucurbita maxima OX=3661 GN=LOC111483427 PE=4 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 4.8e-110
Identity = 211/223 (94.62%), Postives = 213/223 (95.52%), Query Frame = 0


Query: 1   MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60
           MEDWDLQAIVKGCNGSIT SRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD
Sbjct: 1   MEDWDLQAIVKGCNGSITSSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60

Query: 61  SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW 120
           SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSR+NRVVKEVKAEGVCSDSW
Sbjct: 61  SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRQNRVVKEVKAEGVCSDSW 120

Query: 121 GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPTRR 180
           GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFV+TYTAEHNHAEPTRR
Sbjct: 121 GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVITYTAEHNHAEPTRR 180

Query: 181 NALAGTTRKKFPAPENPRLDVILSPSNSMSEASVEEEQPMEGV 224
           NALAGTTRKKFPAPENPRLD        MS ASVEEEQPMEGV
Sbjct: 181 NALAGTTRKKFPAPENPRLD--------MSVASVEEEQPMEGV 215

[Close]

BLAST of Carg22217 vs. ExPASy TrEMBL
Match: A0A6J1FQY6 (probable WRKY transcription factor 29 OS=Cucurbita moschata OX=3662 GN=LOC111447645 PE=4 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 4.4e-79
Identity = 170/238 (71.43%), Postives = 188/238 (78.99%), Query Frame = 0


Query: 1   MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDEL------LSSCSVYDEFEGL 60
           MEDWDLQAIVKGC+     SR  ++++DP   SFL E+D+        SSCS + EFEG 
Sbjct: 1   MEDWDLQAIVKGCS-----SRPTSMLQDP-FFSFLSEEDDFSCDFSSSSSCSSH-EFEG- 60

Query: 61  FGSS-----DDSASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKE 120
           FGSS             ++AAIS LL E KEP+KL +K QI+A KQKQSKKSR+NRVVKE
Sbjct: 61  FGSSIFPVFHPLIPYPNNSAAISDLLGEFKEPQKLLQKKQISAIKQKQSKKSRQNRVVKE 120

Query: 121 VKAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYT 180
           VKA+GVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERS SDP  FVVTYT
Sbjct: 121 VKADGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSFSDPNIFVVTYT 180

Query: 181 AEHNHAEPTRRNALAGTTRKKFPAPENPRLDVILSPSNSMSEASVEEEQPMEGVGRGG 228
           AEHNHAEPTRRNALAGTTRKKFPAPENP  DVILSP++S   AS+EEEQPMEGVG GG
Sbjct: 181 AEHNHAEPTRRNALAGTTRKKFPAPENPSFDVILSPNDSTCVASIEEEQPMEGVGEGG 230

[Close]

BLAST of Carg22217 vs. ExPASy TrEMBL
Match: E7CEX4 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY26 PE=2 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 7.5e-79
Identity = 175/252 (69.44%), Postives = 191/252 (75.79%), Query Frame = 0


Query: 1   MEDWDLQAIVKGCN----GSITPSRTATLMEDPNLVSFL---IEKDELLSSC-------- 60
           MEDW LQAIVKGCN    GS T + T  LMED N  SFL    E+DE  SSC        
Sbjct: 1   MEDWGLQAIVKGCNGIPIGSSTTAATTRLMEDSNWYSFLRSDQEEDEFFSSCVYNSSYNN 60

Query: 61  -------SVYDEFEGLFGSSDDSASASASAAAISGLLRELKEP--EKLHRKSQ-IAATKQ 120
                    +DEFEGLFG +  + SA   AA+IS LLR+ KEP  +KLH K+Q I  TKQ
Sbjct: 61  PQISSSSIFHDEFEGLFGRNSSNNSA---AASISHLLRDFKEPADQKLHHKNQIIQPTKQ 120

Query: 121 KQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVE 180
           KQSKKSR+NRVVKEVKA+ VCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVE
Sbjct: 121 KQSKKSRQNRVVKEVKADKVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVE 180

Query: 181 RSLSDPGAFVVTYTAEHNHAEPTRRNALAGTTRKKFPAPENPRLDVILSPSNSMSEASVE 227
           RSLSDP  F+VTYTAEHNHAEPTRRNALAGTTRKKFPA ENP LD+ILSP+NS S AS+E
Sbjct: 181 RSLSDPEVFIVTYTAEHNHAEPTRRNALAGTTRKKFPALENPNLDMILSPNNSTSVASIE 240

[Close]

BLAST of Carg22217 vs. ExPASy TrEMBL
Match: A0A5D3CZ98 (Putative WRKY transcription factor 29 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004460 PE=4 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 5.4e-77
Identity = 174/254 (68.50%), Postives = 189/254 (74.41%), Query Frame = 0


Query: 1   MEDWDLQAIVKGCN----GSITPSRTATLMEDP-NLVSFLIEKDE--------------- 60
           MEDW LQAIVKGCN    GS T + T  LMED  NL SFL    E               
Sbjct: 1   MEDWGLQAIVKGCNGIPIGSSTTAATTRLMEDTNNLYSFLRSDQEEDGGFFSSCVYNNYY 60

Query: 61  ---LLSSCSVYDEFEGLFGSSDDSASASASAAAISGLLRELKEP--EKLHRKSQ-IAATK 120
              + SS   +DEFEGLFG +  + SA   AA+IS LLR+ KEP  +KLH K+Q I  TK
Sbjct: 61  NPQISSSSIFHDEFEGLFGRNSSNNSA---AASISHLLRDFKEPADQKLHHKNQIIQPTK 120

Query: 121 QKQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQV 180
           QKQSKKSR+NRVVKEVKA+ VCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQV
Sbjct: 121 QKQSKKSRQNRVVKEVKADKVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQV 180

Query: 181 ERSLSDPGAFVVTYTAEHNHAEPTRRNALAGTTRKKFPAPENPRLDVILSPSNSMSEASV 227
           ERSLSDP  FVVTYTAEHNHAEPTRRNALAGTTRKKFPA ENP LD+ILSP+NS S AS+
Sbjct: 181 ERSLSDPEVFVVTYTAEHNHAEPTRRNALAGTTRKKFPALENPNLDMILSPNNSTSVASI 240

[Close]

BLAST of Carg22217 vs. TAIR 10
Match: AT4G23550.1 (WRKY family transcription factor )

HSP 1 Score: 147.5 bits (371), Expect = 1.3e-35
Identity = 102/246 (41.46%), Postives = 137/246 (55.69%), Query Frame = 0


Query: 1   MEDWDLQAIVKGCNGS----------------ITPSRTATLMEDPNLVSFLIEKDELLSS 60
           M++ DL+AIV+G +GS                  P  T++  E     S L E  EL   
Sbjct: 1   MDEGDLEAIVRGYSGSGDAFSGESSGTFSPSFCLPMETSSFYEPEMETSGLDELGEL--- 60

Query: 61  CSVYDEFEGLFGSSDDSASASASAAAISGLLRELKEPEK----LHRKS-----QIAATKQ 120
              Y  F     S+    ++S S    S   R+ K+       L   S     +I+ +K 
Sbjct: 61  ---YKPFYPF--STQTILTSSVSLPEDSKPFRDDKKQRSHGCLLSNGSRADHIRISESKS 120

Query: 121 KQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVE 180
           K+SKK+++ RVV++VK E + SD+W WRKYGQKPIKGSPYPRSYYRCSSSKGC ARKQVE
Sbjct: 121 KKSKKNQQKRVVEQVKEENLLSDAWAWRKYGQKPIKGSPYPRSYYRCSSSKGCLARKQVE 180

Query: 181 RSLSDPGAFVVTYTAEHNHAEPTRRNALAGTTRKKFPAP-----ENPRLDVILSPSNSMS 217
           R+  +P  F +TYT EHNH  PTRRN+LAG+TR K   P     +    +V+ SP+++  
Sbjct: 181 RNPQNPEKFTITYTNEHNHELPTRRNSLAGSTRAKTSQPKPTLTKKSEKEVVSSPTSNPM 238

[Close]

BLAST of Carg22217 vs. TAIR 10
Match: AT4G01250.1 (WRKY family transcription factor )

HSP 1 Score: 141.0 bits (354), Expect = 1.2e-33
Identity = 86/200 (43.00%), Postives = 115/200 (57.50%), Query Frame = 0


Query: 2   EDWDLQAIVKGCNGSITPSRTAT-----LMEDPNLVSFLIEKDELLSSCSVYDEFEGLFG 61
           +DWDL A+V+GC+ +++ S T T     +    N +  +  +   +S   + D +     
Sbjct: 3   DDWDLHAVVRGCS-AVSSSATTTVYSPGVSSHTNPIFTVGRQSNAVSFGEIRDLYTPFTQ 62

Query: 62  SSDDSASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSR------ENRVVKEV 121
            S  S+ +  +        +  K P  L   S    +K   S  SR      +++ V  V
Sbjct: 63  ESVVSSFSCINYPEEPRKPQNQKRPLSLSASSGSVTSKPSGSNTSRSKRRKIQHKKVCHV 122

Query: 122 KAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTA 181
            AE + SD W WRKYGQKPIKGSPYPR YYRCS+SKGC ARKQVER+ SDP  F+VTYTA
Sbjct: 123 AAEALNSDVWAWRKYGQKPIKGSPYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTA 182

Query: 182 EHNHAEPTRRNALAGTTRKK 191
           EHNH  PT RN+LAG+TR+K
Sbjct: 183 EHNHPAPTHRNSLAGSTRQK 201

[Close]

BLAST of Carg22217 vs. TAIR 10
Match: AT5G52830.1 (WRKY DNA-binding protein 27 )

HSP 1 Score: 134.4 bits (337), Expect = 1.2e-31
Identity = 69/123 (56.10%), Postives = 84/123 (68.29%), Query Frame = 0


Query: 95  QKQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQV 154
           + + +K+++ R +  V  E + SD W WRKYGQKPIKGSPYPR+YYRCSSSKGC ARKQV
Sbjct: 143 RSRKRKNQQKRTICHVTQENLSSDLWAWRKYGQKPIKGSPYPRNYYRCSSSKGCLARKQV 202

Query: 155 ERSLSDPGAFVVTYTAEHNHAEPTRRNALAGTTRKKFPAPENPRLDVILSPSNSMSEASV 214
           ERS  DP  F+VTYT EH H  PT RN+LAG+TR K   P NP    +  P  S    +V
Sbjct: 203 ERSNLDPNIFIVTYTGEHTHPRPTHRNSLAGSTRNK-SQPVNP----VPKPDTSPLSDTV 260

Query: 215 EEE 218
           +EE
Sbjct: 263 KEE 260

[Close]

BLAST of Carg22217 vs. TAIR 10
Match: AT2G34830.1 (WRKY DNA-binding protein 35 )

HSP 1 Score: 126.3 bits (316), Expect = 3.2e-29
Identity = 74/166 (44.58%), Postives = 98/166 (59.04%), Query Frame = 0


Query: 59  DDSASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSD 118
           +++ ++S S   IS   R L    +  +  ++       +  SR +        E V SD
Sbjct: 164 NNNNTSSCSQVQISSSPRNLGIKRRKSQAKKVVCIPAPAAMNSRSS-------GEVVPSD 223

Query: 119 SWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPT 178
            W WRKYGQKPIKGSPYPR YYRCSSSKGCSARKQVERS +DP   V+TYT+EHNH  PT
Sbjct: 224 LWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPT 283

Query: 179 RRNALAGTTRKKFPAPENP-----RLDVILSPSNSMSEASVEEEQP 220
           +RNALAG+TR    +  NP           SPS+ + + +  +++P
Sbjct: 284 QRNALAGSTRSSSSSSLNPSSKSSTAAATTSPSSRVFQNNSSKDEP 322

[Close]

BLAST of Carg22217 vs. TAIR 10
Match: AT5G45050.1 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 121.3 bits (303), Expect = 1.0e-27
Identity = 73/144 (50.69%), Postives = 93/144 (64.58%), Query Frame = 0


Query: 77   ELKEPEKL--HRKSQIAATKQ-------KQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQ 136
            E K+ +KL  + +S + ATK+        + K ++E RVV  V      SD W WRKYGQ
Sbjct: 1131 ESKKTDKLVDNIQSSMIATKEIEITRSKSRRKNNKEKRVVCVVDRGSRSSDLWVWRKYGQ 1190

Query: 137  KPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYTAEHNHAEPTRRNALAGTT 196
            KPIK SPYPRSYYRC+SSKGC ARKQVERS +DP   V+TY +EHNH  PT RN LAG+T
Sbjct: 1191 KPIKSSPYPRSYYRCASSKGCFARKQVERSRTDPNVSVITYISEHNHPFPTLRNTLAGST 1250

Query: 197  RKKFPAPENPRLDVILSPSNSMSE 212
            R    +  +   DV  S S+++S+
Sbjct: 1251 R----SSSSKCSDVTTSASSTVSQ 1270

The following BLAST results are available for this feature:

BLAST of Carg22217 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7015174.1	1.3e-125	100.00	WRKY transcription factor 22, partial [Cucurbita argyrosperma subsp. argyrosperm...	[more]
KAG6577177.1	1.2e-118	98.68	WRKY transcription factor 22, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022931440.1	3.4e-118	98.24	probable WRKY transcription factor 29 [Cucurbita moschata]	[more]
XP_022985418.1	1.0e-109	94.62	probable WRKY transcription factor 29 [Cucurbita maxima]	[more]
XP_023552880.1	2.7e-107	91.70	probable WRKY transcription factor 29 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg22217 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SUS1	1.9e-34	41.46	Probable WRKY transcription factor 29 OS=Arabidopsis thaliana OX=3702 GN=WRKY29 ...	[more]
O04609	1.7e-32	43.00	WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1	[more]
Q9FLX8	1.6e-30	56.10	Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 ...	[more]
O64747	4.5e-28	44.58	Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 ...	[more]
Q9FL92	1.4e-26	50.69	Disease resistance protein RRS1B OS=Arabidopsis thaliana OX=3702 GN=RRS1B PE=1 S...	[more]

BLAST of Carg22217 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EZF1	1.7e-118	98.24	probable WRKY transcription factor 29 OS=Cucurbita moschata OX=3662 GN=LOC111437...	[more]
A0A6J1JDJ7	4.8e-110	94.62	probable WRKY transcription factor 29 OS=Cucurbita maxima OX=3661 GN=LOC11148342...	[more]
A0A6J1FQY6	4.4e-79	71.43	probable WRKY transcription factor 29 OS=Cucurbita moschata OX=3662 GN=LOC111447...	[more]
E7CEX4	7.5e-79	69.44	WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY26 PE=2 SV=1	[more]
A0A5D3CZ98	5.4e-77	68.50	Putative WRKY transcription factor 29 OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]

BLAST of Carg22217 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23550.1	1.3e-35	41.46	WRKY family transcription factor	[more]
AT4G01250.1	1.2e-33	43.00	WRKY family transcription factor	[more]
AT5G52830.1	1.2e-31	56.10	WRKY DNA-binding protein 27	[more]
AT2G34830.1	3.2e-29	44.58	WRKY DNA-binding protein 35	[more]
AT5G45050.1	1.0e-27	50.69	Disease resistance protein (TIR-NBS-LRR class)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 116..176 e-value: 1.1E-34 score: 131.2
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 118..174 e-value: 3.7E-25 score: 87.9
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 111..177 score: 32.294022
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 92..178 e-value: 1.7E-28 score: 100.2
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 114..177
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 175..234
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 85..105
None	No IPR available	PANTHER	PTHR32096:SF87	WRKY TRANSCRIPTION FACTOR 63-RELATED	coord: 3..209
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR32096	WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATED	coord: 3..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg22217-RA	Carg22217-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0000976	transcription cis-regulatory region binding
molecular_function	GO:0043565	sequence-specific DNA binding