Carg21828 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg21828
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionpectinesterase inhibitor 12-like
LocationCarg_Chr05: 156392 .. 156886 (+)
RNA-Seq ExpressionCarg21828
SyntenyCarg21828
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCACAACTTCATGCTCTTCGTTGTTTGTGGCCATTATGGCGTGTATGTGCTTCACTGCCCACGCATTTTTCAATCTCAACATTGGAATGGACATCTGCCAGAAGGCGGACTACCCGGCCTTGTGTCGCTCTGTGGCTAGGGGCCAGGCGGACCCATCGGTGGTCCTCGAGGCCACGATCAGGCTATTGATGGTGCAGACGTACAAGGCGATGGGACAGGCCCGGCGCGACAAGACGTCGGCCATGGAAGTTTGCCTGGAGGTTTACGACGACGCGTTCAGCAGCCTGCAGACAGCCATTTCAAACCTGAAAAGCCACGACAAGGGTAGCCTCGGCATTAACCTCTCAGCGGCATTGACCGATTACGTGACCTGCGACGATGCGGTGGAGGAGAGTGGGTTAAGTTCACCGGTTACCCGAAGAAACGCGAGGATGCGCAAAATGACGAGCAACTGCTTGTATTTGTCTAGTTTGATTCGGTGGCACTGA

mRNA sequence

ATGGCTTCCACAACTTCATGCTCTTCGTTGTTTGTGGCCATTATGGCGTGTATGTGCTTCACTGCCCACGCATTTTTCAATCTCAACATTGGAATGGACATCTGCCAGAAGGCGGACTACCCGGCCTTGTGTCGCTCTGTGGCTAGGGGCCAGGCGGACCCATCGGTGGTCCTCGAGGCCACGATCAGGCTATTGATGGTGCAGACGTACAAGGCGATGGGACAGGCCCGGCGCGACAAGACGTCGGCCATGGAAGTTTGCCTGGAGGTTTACGACGACGCGTTCAGCAGCCTGCAGACAGCCATTTCAAACCTGAAAAGCCACGACAAGGGTAGCCTCGGCATTAACCTCTCAGCGGCATTGACCGATTACGTGACCTGCGACGATGCGGTGGAGGAGAGTGGGTTAAGTTCACCGGTTACCCGAAGAAACGCGAGGATGCGCAAAATGACGAGCAACTGCTTGTATTTGTCTAGTTTGATTCGGTGGCACTGA

Coding sequence (CDS)

ATGGCTTCCACAACTTCATGCTCTTCGTTGTTTGTGGCCATTATGGCGTGTATGTGCTTCACTGCCCACGCATTTTTCAATCTCAACATTGGAATGGACATCTGCCAGAAGGCGGACTACCCGGCCTTGTGTCGCTCTGTGGCTAGGGGCCAGGCGGACCCATCGGTGGTCCTCGAGGCCACGATCAGGCTATTGATGGTGCAGACGTACAAGGCGATGGGACAGGCCCGGCGCGACAAGACGTCGGCCATGGAAGTTTGCCTGGAGGTTTACGACGACGCGTTCAGCAGCCTGCAGACAGCCATTTCAAACCTGAAAAGCCACGACAAGGGTAGCCTCGGCATTAACCTCTCAGCGGCATTGACCGATTACGTGACCTGCGACGATGCGGTGGAGGAGAGTGGGTTAAGTTCACCGGTTACCCGAAGAAACGCGAGGATGCGCAAAATGACGAGCAACTGCTTGTATTTGTCTAGTTTGATTCGGTGGCACTGA

Protein sequence

MASTTSCSSLFVAIMACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEATIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAALTDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH
Homology
BLAST of Carg21828 vs. NCBI nr
Match: KAG7029213.1 (hypothetical protein SDJN02_07550, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 321.2 bits (822), Expect = 5.0e-84
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0

Query: 1   MASTTSCSSLFVAIMACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEA 60
           MASTTSCSSLFVAIMACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEA
Sbjct: 1   MASTTSCSSLFVAIMACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEA 60

Query: 61  TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAA 120
           TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAA
Sbjct: 61  TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAA 120

Query: 121 LTDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH 165
           LTDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH
Sbjct: 121 LTDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH 164

BLAST of Carg21828 vs. NCBI nr
Match: KAG6598234.1 (hypothetical protein SDJN03_08012, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 318.2 bits (814), Expect = 4.3e-83
Identity = 163/164 (99.39%), Postives = 163/164 (99.39%), Query Frame = 0

Query: 1   MASTTSCSSLFVAIMACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEA 60
           MASTTSCSSLFVAIMACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEA
Sbjct: 1   MASTTSCSSLFVAIMACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEA 60

Query: 61  TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAA 120
           TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAA
Sbjct: 61  TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAA 120

Query: 121 LTDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH 165
           LTDYVTCDDAVEESGLSS VTRRNARMRKMTSNCLYLSSLIRWH
Sbjct: 121 LTDYVTCDDAVEESGLSSAVTRRNARMRKMTSNCLYLSSLIRWH 164

BLAST of Carg21828 vs. NCBI nr
Match: XP_022961849.1 (uncharacterized protein LOC111462491 [Cucurbita moschata])

HSP 1 Score: 317.8 bits (813), Expect = 5.6e-83
Identity = 162/164 (98.78%), Postives = 163/164 (99.39%), Query Frame = 0

Query: 1   MASTTSCSSLFVAIMACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEA 60
           MASTTSCSSLFVAI+ACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEA
Sbjct: 1   MASTTSCSSLFVAILACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEA 60

Query: 61  TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAA 120
           TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAA
Sbjct: 61  TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAA 120

Query: 121 LTDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH 165
            TDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH
Sbjct: 121 STDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH 164

BLAST of Carg21828 vs. NCBI nr
Match: XP_023547120.1 (uncharacterized protein LOC111806025 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 308.1 bits (788), Expect = 4.4e-80
Identity = 156/164 (95.12%), Postives = 159/164 (96.95%), Query Frame = 0

Query: 1   MASTTSCSSLFVAIMACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEA 60
           MASTTSCSSL VAIMACMCFTAHAFFNL IGMDICQKADYP LCRSVARG+ DPSVVLEA
Sbjct: 1   MASTTSCSSLLVAIMACMCFTAHAFFNLKIGMDICQKADYPVLCRSVARGREDPSVVLEA 60

Query: 61  TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAA 120
           TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQT+ISN+KSHDKGSLGINLSAA
Sbjct: 61  TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTSISNMKSHDKGSLGINLSAA 120

Query: 121 LTDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH 165
           LTDYVTCDDAVEESGLSSPVTRRN RMRKMTSNCLYLSSLIRWH
Sbjct: 121 LTDYVTCDDAVEESGLSSPVTRRNVRMRKMTSNCLYLSSLIRWH 164

BLAST of Carg21828 vs. NCBI nr
Match: XP_022996861.1 (pectinesterase inhibitor 12-like [Cucurbita maxima])

HSP 1 Score: 305.4 bits (781), Expect = 2.9e-79
Identity = 155/164 (94.51%), Postives = 158/164 (96.34%), Query Frame = 0

Query: 1   MASTTSCSSLFVAIMACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEA 60
           MASTTSCSSLFVAIMACMCFTAHAFFNLNIG+DICQKADYPALCRSVARGQ DPSVVLE 
Sbjct: 1   MASTTSCSSLFVAIMACMCFTAHAFFNLNIGVDICQKADYPALCRSVARGQKDPSVVLET 60

Query: 61  TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAA 120
           TIRLLM QTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQT+IS+LKSHDKGSLGINLSAA
Sbjct: 61  TIRLLMTQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTSISSLKSHDKGSLGINLSAA 120

Query: 121 LTDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH 165
           LTDYVTCDDAV ESGL SPVTRRN RMRKMTSNCLYLSSLIRWH
Sbjct: 121 LTDYVTCDDAVAESGLRSPVTRRNERMRKMTSNCLYLSSLIRWH 164

BLAST of Carg21828 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.1e-08
Identity = 32/76 (42.11%), Postives = 50/76 (65.79%), Query Frame = 0

Query: 87  CLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAALTDYVTCDDAV-EESGLSSPVTRRNA 146
           C+ +Y DA SS+Q AI++ KS D  S  + +SAAL D VTC+D   E+ G++SPVT+ N 
Sbjct: 93  CVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIASPVTKENK 152

Query: 147 RMRKMTSNCLYLSSLI 162
              ++T+  L ++ L+
Sbjct: 153 DYVQLTAISLAITKLL 168

BLAST of Carg21828 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 4.7e-08
Identity = 32/76 (42.11%), Postives = 49/76 (64.47%), Query Frame = 0

Query: 87  CLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAALTDYVTCDDAV-EESGLSSPVTRRNA 146
           C+ +Y DA SS+Q AI++ KS D  S  + +SAAL D VTC+D   E+ G+ SPVT+ N 
Sbjct: 102 CVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENK 161

Query: 147 RMRKMTSNCLYLSSLI 162
              ++T+  L ++ L+
Sbjct: 162 DYVQLTAISLAITKLL 177

BLAST of Carg21828 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.0e-06
Identity = 29/75 (38.67%), Postives = 44/75 (58.67%), Query Frame = 0

Query: 75  QARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAALTDYVTCDDAVEES 134
           Q  +D    +++CL  Y+DA   L TA++N+KS D     INLSAAL     C+D  +E+
Sbjct: 88  QRYQDIVEDLKLCLGFYNDANDDLTTALANIKSRDYQGANINLSAALDVPGNCEDDFKEA 147

Query: 135 GLSSPVTRRNARMRK 150
             +SP+T  N+ + K
Sbjct: 148 KKTSPITNENSILFK 162

BLAST of Carg21828 vs. ExPASy TrEMBL
Match: A0A6J1HF79 (uncharacterized protein LOC111462491 OS=Cucurbita moschata OX=3662 GN=LOC111462491 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 2.7e-83
Identity = 162/164 (98.78%), Postives = 163/164 (99.39%), Query Frame = 0

Query: 1   MASTTSCSSLFVAIMACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEA 60
           MASTTSCSSLFVAI+ACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEA
Sbjct: 1   MASTTSCSSLFVAILACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEA 60

Query: 61  TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAA 120
           TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAA
Sbjct: 61  TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAA 120

Query: 121 LTDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH 165
            TDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH
Sbjct: 121 STDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH 164

BLAST of Carg21828 vs. ExPASy TrEMBL
Match: A0A6J1K5X6 (pectinesterase inhibitor 12-like OS=Cucurbita maxima OX=3661 GN=LOC111491980 PE=4 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.4e-79
Identity = 155/164 (94.51%), Postives = 158/164 (96.34%), Query Frame = 0

Query: 1   MASTTSCSSLFVAIMACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEA 60
           MASTTSCSSLFVAIMACMCFTAHAFFNLNIG+DICQKADYPALCRSVARGQ DPSVVLE 
Sbjct: 1   MASTTSCSSLFVAIMACMCFTAHAFFNLNIGVDICQKADYPALCRSVARGQKDPSVVLET 60

Query: 61  TIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAA 120
           TIRLLM QTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQT+IS+LKSHDKGSLGINLSAA
Sbjct: 61  TIRLLMTQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTSISSLKSHDKGSLGINLSAA 120

Query: 121 LTDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH 165
           LTDYVTCDDAV ESGL SPVTRRN RMRKMTSNCLYLSSLIRWH
Sbjct: 121 LTDYVTCDDAVAESGLRSPVTRRNERMRKMTSNCLYLSSLIRWH 164

BLAST of Carg21828 vs. ExPASy TrEMBL
Match: A0A5A7V5S8 (Putative pectinesterase/pectinesterase inhibitor 40 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G002070 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 2.2e-45
Identity = 94/168 (55.95%), Postives = 127/168 (75.60%), Query Frame = 0

Query: 3   STTSCSSLFVAIMACMCF------TAHAFFNLNIGMDICQKADYPALCRSVARGQADPSV 62
           ST  C++ ++ ++            A    NLN+ +D+C+KADYPALCRSV +G  DPS 
Sbjct: 8   STVCCNNFYLPLLVVAMLWSSPGSEAALTLNLNLSLDLCKKADYPALCRSVVKGLTDPST 67

Query: 63  VLEATIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGIN 122
            +E +I+ LMV+T +AM  A+R K+SAM+VC+EVYDDA+S+L+T++S+LKSHDKG+L IN
Sbjct: 68  AMETSIKKLMVETKQAMSVAKRQKSSAMDVCIEVYDDAYSNLETSLSSLKSHDKGTLNIN 127

Query: 123 LSAALTDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH 165
           LSA+LTDYVTCDDA+ E G +SPVTR N  +R+MT+NCLYLS LIR H
Sbjct: 128 LSASLTDYVTCDDAIAERGSTSPVTRNNKLLREMTTNCLYLSGLIRLH 175

BLAST of Carg21828 vs. ExPASy TrEMBL
Match: A0A1S3BAX0 (uncharacterized protein LOC103487709 OS=Cucumis melo OX=3656 GN=LOC103487709 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 2.2e-45
Identity = 94/168 (55.95%), Postives = 127/168 (75.60%), Query Frame = 0

Query: 3   STTSCSSLFVAIMACMCF------TAHAFFNLNIGMDICQKADYPALCRSVARGQADPSV 62
           ST  C++ ++ ++            A    NLN+ +D+C+KADYPALCRSV +G  DPS 
Sbjct: 8   STVCCNNFYLPLLVVAMLWSSPGSEAALTLNLNLSLDLCKKADYPALCRSVVKGLTDPST 67

Query: 63  VLEATIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGIN 122
            +E +I+ LMV+T +AM  A+R K+SAM+VC+EVYDDA+S+L+T++S+LKSHDKG+L IN
Sbjct: 68  AMETSIKKLMVETKQAMSVAKRQKSSAMDVCIEVYDDAYSNLETSLSSLKSHDKGTLNIN 127

Query: 123 LSAALTDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH 165
           LSA+LTDYVTCDDA+ E G +SPVTR N  +R+MT+NCLYLS LIR H
Sbjct: 128 LSASLTDYVTCDDAIAERGSTSPVTRNNKLLREMTTNCLYLSGLIRLH 175

BLAST of Carg21828 vs. ExPASy TrEMBL
Match: A0A0A0LNC2 (Invertase/pectin methylesterase inhibitor family protein OS=Cucumis sativus OX=3659 GN=Csa_2G348980 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 5.0e-45
Identity = 97/171 (56.73%), Postives = 131/171 (76.61%), Query Frame = 0

Query: 2   ASTTSCSS---LFVAIMACMCFT-----AHAFFNLNIGMDICQKADYPALCRSVARGQAD 61
           ++ +SCS+   L + ++A M ++     A    NLN+ +D+C+KADYPALCRSV +G  D
Sbjct: 8   STISSCSNFYLLMLLVVANMLWSSWGSEAALTLNLNLSLDLCKKADYPALCRSVVKGLVD 67

Query: 62  PSVVLEATIRLLMVQTYKAMGQARRDKTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSL 121
           PS  +E +I+ LMV+T +AM  A+R K+SAM+VC+EVYDDA+S+L+T +S+LKSHDKG+L
Sbjct: 68  PSTAMETSIKQLMVETKQAMSVAKRQKSSAMDVCIEVYDDAYSNLETCLSSLKSHDKGTL 127

Query: 122 GINLSAALTDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIRWH 165
            INLSAALTDYVTC DA+ E GLSSP+TR N  + +MT+NCLYLS LIR H
Sbjct: 128 NINLSAALTDYVTCQDAIAEKGLSSPITRNNNLLSQMTTNCLYLSGLIRLH 178

BLAST of Carg21828 vs. TAIR 10
Match: AT1G70540.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 99.8 bits (247), Expect = 2.2e-21
Identity = 53/134 (39.55%), Postives = 79/134 (58.96%), Query Frame = 0

Query: 31  GMDICQKADYPALCRSVARGQADPSVVLEATIRLLMVQTYKAMGQAR--RDKTSAMEVCL 90
           G D C  +D+  LCRSV +GQ + +   E +IR LM +T KA   A+  R     ++ C 
Sbjct: 33  GRDPCSVSDFKVLCRSVVKGQKNVNAATEVSIRELMKRTIKAKEAAKISRKSGGGLKTCY 92

Query: 91  EVYDDAFSSLQTAISNLKSHDKGSLGINLSAALTDYVTCDDAVEESGLSSPVTRRNARMR 150
             YD A  +LQ A+ N+K +D  SL INLSA+LTD+ TC+DA+     S+   +  + + 
Sbjct: 93  SNYDSALENLQKALKNIKQNDGFSLNINLSASLTDFDTCNDAMGGGTASNVFAKSTSTLH 152

Query: 151 KMTSNCLYLSSLIR 163
           +M  NCL LS+L++
Sbjct: 153 EMADNCLALSTLVK 166

BLAST of Carg21828 vs. TAIR 10
Match: AT1G23350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 94.4 bits (233), Expect = 9.3e-20
Identity = 52/154 (33.77%), Postives = 92/154 (59.74%), Query Frame = 0

Query: 11  FVAIMACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEATIRLLMVQTY 70
           +V I   +  T+    ++++  D C  +D+PALCRS  +GQ + +   +  I+ LM +T 
Sbjct: 6   YVTIFLLLAATSSQALSISLA-DPCTVSDFPALCRSTIKGQTNVNAATDVAIKELMKRTR 65

Query: 71  KAMGQARRD--KTSAMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLSAALTDYVTCD 130
           +A   A ++  +   +  CL  ++ AF +L+ A++N+K +D  SL INLSAALTDY TC 
Sbjct: 66  QAKKIAEKELKRDGGVATCLSNFNSAFDNLEKALTNIKENDGFSLNINLSAALTDYDTCS 125

Query: 131 DAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIR 163
           D+++E+   + + +    + +M  NCL LS+L++
Sbjct: 126 DSMKETKEVNVIYKSAGVLYRMADNCLALSTLVK 158

BLAST of Carg21828 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 84.7 bits (208), Expect = 7.4e-17
Identity = 55/135 (40.74%), Postives = 76/135 (56.30%), Query Frame = 0

Query: 34  ICQKADYPALCRSVARGQADP------SVVLEATIRLLMVQTYKAMGQARRDKTSAMEVC 93
           IC K D P LC S       P      S VL   I+  +  T  AM  A  +K  A + C
Sbjct: 133 ICGKTDNPPLCESSVSPLLTPQLKPNTSSVLILAIQASITATKAAM--AIVEKVDASD-C 192

Query: 94  LEVYDDAFSSLQTAISNLKSHDKGSLGINLSAALTDYVTCDDAVEESGLSSPVTRRNARM 153
            E+YDDA  +L+ A++ +KS D  ++  NLSAA+TDY TC+D  EESG  +P+     ++
Sbjct: 193 QELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGFEESGEPNPLAYVADKL 252

Query: 154 RKMTSNCLYLSSLIR 163
            KM SNCL +S+LI+
Sbjct: 253 TKMVSNCLAISTLIK 264

BLAST of Carg21828 vs. TAIR 10
Match: AT1G10770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 72.8 bits (177), Expect = 2.9e-13
Identity = 42/133 (31.58%), Postives = 69/133 (51.88%), Query Frame = 0

Query: 32  MDICQKADYPALCRSVARGQADPSVVLEATIRLLMVQTYKAMGQARRDK--TSAMEVCLE 91
           ++IC    YP+LCR + +    P      TI+ L  +T  A+ +  R K    A+  C E
Sbjct: 35  INICSHTAYPSLCRPLVKRVTSPRKATHRTIQALEAKTKLALAETARFKNGNQAVSTCYE 94

Query: 92  VYDDAFSSLQTAISNLKSHDKGSLGINLSAALTDYVTCDDAVEESGLSSPVTRRNARMRK 151
              DA  +L +A  +++  D  ++   L+AA++DY  C D   E+   + +      +RK
Sbjct: 95  TLGDAVYNLASARKSIRKRDVPAMNTYLTAAVSDYGACVDGFIETQQVNAIQNAVVDLRK 154

Query: 152 MTSNCLYLSSLIR 163
           ++SNCL LS+LIR
Sbjct: 155 ISSNCLALSTLIR 167

BLAST of Carg21828 vs. TAIR 10
Match: AT1G60760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 55.8 bits (133), Expect = 3.7e-08
Identity = 44/164 (26.83%), Postives = 80/164 (48.78%), Query Frame = 0

Query: 1   MASTTSCSSLFVAIMACMCFTAHAFFNLNIGMDICQKADYPALCRSVARGQADPSVVLEA 60
           MAS  +       ++A +   A +         +C +  YP LCR + +G  +P     +
Sbjct: 1   MASRNTIQMYLTTVVAIVILLAASQATEARFTSLCAQTAYPTLCRPLVKG-PNPRRATHS 60

Query: 61  TIRLLMVQTYKAMGQARRDKTS--AMEVCLEVYDDAFSSLQTAISNLKSHDKGSLGINLS 120
           TIR L  +T  A+  + R K+    + +C     DA  +L+ A ++++  +   L + L+
Sbjct: 61  TIRALEAKTKLAITDSARFKSGNPTVAICYATLVDASFNLRRARTSIRKRNVMMLKMFLT 120

Query: 121 AALTDYVTCDDAVEESGLSSPVTRRNARMRKMTSNCLYLSSLIR 163
           AA++DY  C +   +S   + +      +RKM +NCL L++LIR
Sbjct: 121 AAVSDYGVCVNGFIDSHQVNTLQNDVDELRKMGTNCLLLATLIR 163

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029213.15.0e-84100.00hypothetical protein SDJN02_07550, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6598234.14.3e-8399.39hypothetical protein SDJN03_08012, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022961849.15.6e-8398.78uncharacterized protein LOC111462491 [Cucurbita moschata][more]
XP_023547120.14.4e-8095.12uncharacterized protein LOC111806025 [Cucurbita pepo subsp. pepo][more]
XP_022996861.12.9e-7994.51pectinesterase inhibitor 12-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A9YUH42.1e-0842.11Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1[more]
Q8GT414.7e-0842.11Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1[more]
Q9FJR52.0e-0638.67Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HF792.7e-8398.78uncharacterized protein LOC111462491 OS=Cucurbita moschata OX=3662 GN=LOC1114624... [more]
A0A6J1K5X61.4e-7994.51pectinesterase inhibitor 12-like OS=Cucurbita maxima OX=3661 GN=LOC111491980 PE=... [more]
A0A5A7V5S82.2e-4555.95Putative pectinesterase/pectinesterase inhibitor 40 OS=Cucumis melo var. makuwa ... [more]
A0A1S3BAX02.2e-4555.95uncharacterized protein LOC103487709 OS=Cucumis melo OX=3656 GN=LOC103487709 PE=... [more]
A0A0A0LNC25.0e-4556.73Invertase/pectin methylesterase inhibitor family protein OS=Cucumis sativus OX=3... [more]
Match NameE-valueIdentityDescription
AT1G70540.12.2e-2139.55Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT1G23350.19.3e-2033.77Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT3G36659.17.4e-1740.74Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT1G10770.12.9e-1331.58Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT1G60760.13.7e-0826.83Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 26..157
e-value: 2.9E-25
score: 99.9
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 32..159
e-value: 5.9E-16
score: 56.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 33..156
e-value: 2.5E-21
score: 76.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 32..162
e-value: 3.9E-24
score: 87.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 33..161
NoneNo IPR availablePANTHERPTHR31080PECTINESTERASE INHIBITOR-LIKEcoord: 33..161
NoneNo IPR availablePANTHERPTHR31080:SF68PECTINESTERASE INHIBITOR-LIKEcoord: 33..161
NoneNo IPR availableCDDcd15800PMEI-like_2coord: 32..161
e-value: 1.67323E-44
score: 141.728

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg21828-RACarg21828-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
molecular_function GO:0004857 enzyme inhibitor activity