Carg20746 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg20746
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionMethylenetetrahydrofolate reductase
LocationCarg_Chr15: 10110121 .. 10115615 (+)
RNA-Seq ExpressionCarg20746
SyntenyCarg20746
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGTGATTGACAAGATCACGGCAGCCACCGCCGGTGATGGCGGCAGAGTTGTGTTCTCCTTTGAGTACTTCCCGCCCAAGACGGAGGACGGTGTTGATAACCTCTTCGACAGGATGGACCGGATGGTGGCTCATAATCCTTCCTTCTGTGACATTACTTGGGGCGCCGGTGGCTCCACCGCCGATCTCACCCTCGATATTGCTAACAAGATGCAGAACATGATCTGTGTCGAGACCATGATGCATCTTACCTGCACCAATATGCCTGTTGAGAAGATCGACCATGCTCTTCAGACTATTAAGTCTAATGGCCTTCAGAATGTCCTCGCGCTTCGTGGCGACCCTCCTCATGGACAGGATAAATTCGTCCAGATCGAAGGCGGCTTCGCTTGTGCTCTCGATCTTGTACTCTACAATGGCTTCATCCATCTTCGTTTCCTCTGTTCTGTGTTTCTGCTCTCTAAATGTTTACATTTCTTATTCGTAGGTTAAACACATCAGGGGGAAGTATGGTGACTACTTCGGCATTACTGTAGCTGGATATCCAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTATCGAAATTTACAGATCTACTTGCAAATGCCTTTTCTTTTCTGTAACTAACGGAGTGCTAATACATAGTTCTATTCTATTCTTCTCAGAGGCACATCCGGACGTGATTGGAAGCAATGGCGTGGCGACGGCTGAAGGCTACCAGAGCGATCTGGCTTATCTTAAGAAAAAGGTGAATCTGATCTTTAAGCAATTTAATTCCTCTTCCCCTGTTCTTACATGGATTACTTTTTATATGCTATTTTGTCTGTTCTTTTCTTTTTTTCGTCGATTGCTGTTATTTACTACATTGTCTTTGGTTCTTTTCAGTTTGATGCCGGGGCTGATCTGATTGTTACCCAACTCTTCTATGACACTGACATTTTCCTCAAGTTTGTTAACGATTGTCGTCAAATTGGAATTACTTGTCCGATTGTACCTGGTATTATGCCCATCAATAATTACAAAGGTTTCTTACGCATGACCAGCTTTTGCAAAACGAAGGTACTAACCTCTCTCTAATCTTTTTATTAATGGCCTTGATTTAAATTAAGTGCGTCCACTCTAGAAAAATTTACAACCAGCGATCATCAAAATTTTGCCTTTTCTTCTCCAAAGAAATATTAATATTATCTGTAATGTTAAATTGTTTCTCTTTTACCTGTATGTATGACTTTCGAAAAATTGCAGATTCCGGATGAGGTCATGGCTGCCTTAGAGCCAATCAAGGATAACGAAGAAGCTGTCAAAGCTTATGGAATTCACCTTGGAACTGAAATGTGCAAGAAGATTTTGGCTCATGGAATCAAGACTCTTCACATGTATACACTGAATATGGAGAAATCGGCATTGGCTATACTAATGGTATATTCCAGTATCCTAAACGCATTCTATTTTTACTTCCAATTATTGTTTCTATGGGTAATTTTGTCATTGGATGCTCTGGTACCAGGGGAATTTCTGAAGCTTTTGTTGATGTATTCTTATATTCTGGTGTGTCGGTGAGGGGTTTCCTCTTATGGATTTTGGCTTCCTTTCCATTCGTACTCTTGGGTTCATGTTTTGTTTGAACTAACTGAGACTCGGCATCTTCCTTTAGAAGTAAACTTGTAAGGAGCCATAAAGTAATTTCCATATGAAAACTTCTGATTGTAAATAGATGATAAGTCTATTAAAAGTATGTTCTATGCTGATACACTTATCTTATACTCAAAGGAACATGATACGCACACATACATATATTTTTAAACATAGGGTAAATAATACTATAACATGAGATAGTTTTTTTGTTGATTGGATGCCGTAGGCTTCTTTTTCTATTATAATGGCACTTAAATTTTGTTCTTCTTTTACTTTTTTCGTACATCATTTTTTATCTTTTTTTTTTTTTTTTTTAATCTTTTTTGGAGGCTTGTAGTTTAATCTGTTTTTTTTGGGGTTAAGTCTAAGTGCATTTCTCTACTCATGGCAGAATCTTGGTCTGATTGAAGAGAGCAAAGTCTCAAGATCATTACCTTGGAGACGCCCTACAAATGTTTTCCGTATCAGGGAAGATGTTCGCCCAATCTTCTGGTATCTCTTATGCTTATTGCAAAATGAGGATGCATTCCTAATGATTTTGGATGCATAAAATTGTTTTACATTTTGCTAAATTTGAGATAGAGCTGATCTAGGTAGTTGTTGATCACAGGGCTAATCGTCCTAGAAGCTACATATCAAGGACCATAGGTTGGGACCAGTATCCTCATGGTAGATGGAGTGATTCTAGTAATCCTTCATATGGGGCACTAACTGACTATCAGGTATGAACAATAAACTAGAACTTGTAAATGATCTATGTTGATATTTGTTACATACACACACACTCTAGAGAGATAGAGAGACAGAGAGAGCTGTACGGAGGAGATTTCCTTTTAACATCTGGAATTATTTCTTGTGGACTGCCTGAAAACTTAGTCTTCTGTTCCCTCTTTAATCACCATAGCTTGTAATTGTTAACTCTTTGTGATCTGCATGAATTCAAGACTTAAAAGTAGGCTTTGAGAAATTTCTCCCGCATGATGTATCATTTACTTTGGCTTGGTCTGATTCATCTGACACCACAACATTATCAAAATATAATTCTGTTGAGTCGAATGAAGCTTATTGTAGGACAAGATGTTAATTTTAAAGAAATTCGGTCTCAAATCCTGTTGAAAGCTTCTGGTAAAATTTCCCCCAGATAATTTGTTTGTTTCTTTGGTTATTATAGGCAAGCTGGTGATATGATTTCTTTTTCATGTTTCTCAAACGCTTCAAAGAGCTGAAGATTCAAACTTTACTTCATTCAATATTTATTTGAATATTGTTATAACACGTTTAGCATAGTTTCTAGTGTGTTTAATCAGTGTTACTTTTGCTTTGTTTTGCACTTAAGTTTATGCGGCCACGTGCACGTGACAAGAAGCTTCTTGAGGAATGGGCTCTCCCATTGAAAAGCATAGAGGATGTTTATGAGGTACTTTTCCTAACCAAGGTGTGAAGAAAGTTTGTTATAATGTTAGCGATTGACTAGATATGTTATACTGTTTCCTATCAGAAATTTATGAAGTACTGCCTTGGAAAGTTGAGAAGCAGTCCGTGGTCTGAATTAGACGGTCTTCAGGCTGAGACAAGAATAATAAGCGAACAGCTTGGTAAAATTAACATGAAAGGTTTCCTTTCAATCAACAGCCAACCAGCGGTTAATGGAGAAAAATCTGATTCTCCTTCTGTTGGTAAGTACAAATTAATCCATTCATACAATTATCTTTTTTGACAGCATAACTGAGTAAGTATTTGAAAGTACTGCAATACAATCTGAGGTTAGTGGATAATGCTCTCTTCTTACCATTTCAAGTGTGGACGATTTCATCATTGTTCACCCTATAACTGTTGAAGTCAAAGGAAAAGTACTGAAATACTAAAACTGAACTAGATAATTTTTTATCATGTAGCAAATTCATATTTGGCTAAAAAAACTAAATAAATAAAAACAATATATTATTGGCTCACCAATTGATAAATTTTAGTTTATTACATTATTTTATAAATAATGTTTGATTATTAATTATTAATATACTAATTCATAGGACCCTTTGACTGGCTAGTAAATTCACAAGTTAATGTATGAAGAATAATGAATGGTTTAAATTCTATTTTTTTCCTTGTAATTTCATGTTTGTTTCACAGGTCCCTGAACTTTTAAATAAGTGTTTTAGTTCTCAACTTTCAATTATTCCGTTTTAATCCTTGAACATCCTCTTTTTTTTTTTTTTTTTTTTTTGTCTTTACTGGGGACTTGCTTTAAAACCAAAGCAAGTTAAATACGAAGAAATCATGCTATCAACATGCATCGAGTGATCTAATATAGAATTTCTTGGAACCTTCCCATAGATTTTCATTAAACATAATTTAGTATCAGGGATTGAGGGAGAATTTAGTATGCCCAAGGGAATGGAGCCCACTGCAGTTTCCGTTCATATTCCTCTTGAAAATCAGAAACCATTGTTGGACCTATCTCTCAAATACTACTAACGGTTATTTGAAGAGATGAAAAGCGTGTTGACAGTATCCTACTAAAATTGAATTCTTTGGCCTTTACCCACTTTGGAACCAGTTGGAAATTAATATGTAGTGGAACACGTTGTTCCTCTAATTGAACAACTAAGCATGATATCTTGATAGAAGTGTAAATTTTAACCATAAATAGAAAACACCCTAACAAAACAATCATTTGTATGCAAGGGATCTGAGTCGCCTGTCTAGAGAGGGGGGGTTTCTTCCCCTGTGCATCTTTTGTTTATTTGTTTAGTTTTGTAGTGATCAATTCAGTTTTGACTTATGATATTTGTGGCAATGGCGGCAGAATGCTTGTTTAGTCATCTGATCAGTGTTGGGATGATTAATAATGATTTGCAGGATGGGGTGGATCAGGAGGTTACGTTTATCAAAAGGCATACATCGAGTTTTTCTGTTCCAGGGAAAACTTGGATGCTGTTGTTGACAAATGCAGGGGCCTTCCATCTCTAACCTACATGGCAGTGAATAAGGAAGGCATTTGGGTATCCAACATCACTCAAAATGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCGTCCAGCCAACTGTCGTCGATCCTGCCAGCTTTCTAGTTTGGAAAGATGAGGCATTTGAGATTTGGTCAAGGGGATGGGCTTCCTTGTACCCCGAGGGTGACCCGTCCCGAAAATTTCTCGAAGAGGTTGGTACACTTGTTATATCTTTTCAGTGCTCAATTAATTTCCCCGTTCTTTCTTCACCTGATATTACCCCTTAGTAGAGAAGTGTGGATTATTAAGTGATGGAAATGCGTTGAATCGAAATTTATGTTGTCATCGAAAGGCTTAATCAGGAAGTGCTGTTTTATCTATAAAAGGTTAATCTTAATATAGTGATGGAAGTTGAGAAGTTTTGTGGAAGATTACTATCTCGATGACAAAGCACTTTACTATTTGTTTCAGATACAGAGCAGCTATTACTTGGTGAGCTTGGTGGATAACAATTACGTCAATTCTAACCTCTTTGCTGCTTTGGAGGGATTCTGAGGTTGAGCTGCTATTCCTTTCCATGATTCTAGTTTTACTTCCATCTTTCGATCTCTCATTGAAACCTTGCCCGAGGTTAATGCAAGAAGAATTTTCTTTTTCCGTTTGCTTGAGTTTAATAATATGATTCCCAATAAGGGCTGTCCATTTATTAAGAACATTGGGATTTTAAAACTTCCCGAGGTTGAAGGTAATGTGTCATTCATTTGTGACTAAAAACATTAGATTAAAATATTTCTTAAGGTAGCCATAGCCTTGGGCATGGGATACTATATTATATGGTTTGTAGAATTTTCAATTTTTAACGTGTTTATCATTGG

mRNA sequence

ATGAAGGTGATTGACAAGATCACGGCAGCCACCGCCGGTGATGGCGGCAGAGTTGTGTTCTCCTTTGAGTACTTCCCGCCCAAGACGGAGGACGGTGTTGATAACCTCTTCGACAGGATGGACCGGATGGTGGCTCATAATCCTTCCTTCTGTGACATTACTTGGGGCGCCGGTGGCTCCACCGCCGATCTCACCCTCGATATTGCTAACAAGATGCAGAACATGATCTGTGTCGAGACCATGATGCATCTTACCTGCACCAATATGCCTGTTGAGAAGATCGACCATGCTCTTCAGACTATTAAGTCTAATGGCCTTCAGAATGTCCTCGCGCTTCGTGGCGACCCTCCTCATGGACAGGATAAATTCGTCCAGATCGAAGGCGGCTTCGCTTGTGCTCTCGATCTTGTTAAACACATCAGGGGGAAGTATGGTGACTACTTCGGCATTACTGTAGCTGGATATCCAGAGGCACATCCGGACGTGATTGGAAGCAATGGCGTGGCGACGGCTGAAGGCTACCAGAGCGATCTGGCTTATCTTAAGAAAAAGTTTGATGCCGGGGCTGATCTGATTGTTACCCAACTCTTCTATGACACTGACATTTTCCTCAAGTTTGTTAACGATTGTCGTCAAATTGGAATTACTTGTCCGATTGTACCTGGTATTATGCCCATCAATAATTACAAAGGTTTCTTACGCATGACCAGCTTTTGCAAAACGAAGATTCCGGATGAGGTCATGGCTGCCTTAGAGCCAATCAAGGATAACGAAGAAGCTGTCAAAGCTTATGGAATTCACCTTGGAACTGAAATGTGCAAGAAGATTTTGGCTCATGGAATCAAGACTCTTCACATGTATACACTGAATATGGAGAAATCGGCATTGGCTATACTAATGAATCTTGGTCTGATTGAAGAGAGCAAAGTCTCAAGATCATTACCTTGGAGACGCCCTACAAATGTTTTCCGTATCAGGGAAGATGTTCGCCCAATCTTCTGGGCTAATCGTCCTAGAAGCTACATATCAAGGACCATAGGTTGGGACCAGTATCCTCATGGTAGATGGAGTGATTCTAGTAATCCTTCATATGGGGCACTAACTGACTATCAGTTTATGCGGCCACGTGCACGTGACAAGAAGCTTCTTGAGGAATGGGCTCTCCCATTGAAAAGCATAGAGGATGTTTATGAGAAATTTATGAAGTACTGCCTTGGAAAGTTGAGAAGCAGTCCGTGGTCTGAATTAGACGGTCTTCAGGCTGAGACAAGAATAATAAGCGAACAGCTTGGTAAAATTAACATGAAAGGTTTCCTTTCAATCAACAGCCAACCAGCGGTTAATGGAGAAAAATCTGATTCTCCTTCTGTTGGATGGGGTGGATCAGGAGGTTACGTTTATCAAAAGGCATACATCGAGTTTTTCTGTTCCAGGGAAAACTTGGATGCTGTTGTTGACAAATGCAGGGGCCTTCCATCTCTAACCTACATGGCAGTGAATAAGGAAGGCATTTGGGTATCCAACATCACTCAAAATGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCGTCCAGCCAACTGTCGTCGATCCTGCCAGCTTTCTAGTTTGGAAAGATGAGGCATTTGAGATTTGGTCAAGGGGATGGGCTTCCTTGTACCCCGAGGGTGACCCGTCCCGAAAATTTCTCGAAGAGATACAGAGCAGCTATTACTTGGTGAGCTTGGTGGATAACAATTACGTCAATTCTAACCTCTTTGCTGCTTTGGAGGGATTCTGAGGTTGAGCTGCTATTCCTTTCCATGATTCTAGTTTTACTTCCATCTTTCGATCTCTCATTGAAACCTTGCCCGAGGTTAATGCAAGAAGAATTTTCTTTTTCCGTTTGCTTGAGTTTAATAATATGATTCCCAATAAGGGCTGTCCATTTATTAAGAACATTGGGATTTTAAAACTTCCCGAGGTTGAAGGTAATGTGTCATTCATTTGTGACTAAAAACATTAGATTAAAATATTTCTTAAGGTAGCCATAGCCTTGGGCATGGGATACTATATTATATGGTTTGTAGAATTTTCAATTTTTAACGTGTTTATCATTGG

Coding sequence (CDS)

ATGAAGGTGATTGACAAGATCACGGCAGCCACCGCCGGTGATGGCGGCAGAGTTGTGTTCTCCTTTGAGTACTTCCCGCCCAAGACGGAGGACGGTGTTGATAACCTCTTCGACAGGATGGACCGGATGGTGGCTCATAATCCTTCCTTCTGTGACATTACTTGGGGCGCCGGTGGCTCCACCGCCGATCTCACCCTCGATATTGCTAACAAGATGCAGAACATGATCTGTGTCGAGACCATGATGCATCTTACCTGCACCAATATGCCTGTTGAGAAGATCGACCATGCTCTTCAGACTATTAAGTCTAATGGCCTTCAGAATGTCCTCGCGCTTCGTGGCGACCCTCCTCATGGACAGGATAAATTCGTCCAGATCGAAGGCGGCTTCGCTTGTGCTCTCGATCTTGTTAAACACATCAGGGGGAAGTATGGTGACTACTTCGGCATTACTGTAGCTGGATATCCAGAGGCACATCCGGACGTGATTGGAAGCAATGGCGTGGCGACGGCTGAAGGCTACCAGAGCGATCTGGCTTATCTTAAGAAAAAGTTTGATGCCGGGGCTGATCTGATTGTTACCCAACTCTTCTATGACACTGACATTTTCCTCAAGTTTGTTAACGATTGTCGTCAAATTGGAATTACTTGTCCGATTGTACCTGGTATTATGCCCATCAATAATTACAAAGGTTTCTTACGCATGACCAGCTTTTGCAAAACGAAGATTCCGGATGAGGTCATGGCTGCCTTAGAGCCAATCAAGGATAACGAAGAAGCTGTCAAAGCTTATGGAATTCACCTTGGAACTGAAATGTGCAAGAAGATTTTGGCTCATGGAATCAAGACTCTTCACATGTATACACTGAATATGGAGAAATCGGCATTGGCTATACTAATGAATCTTGGTCTGATTGAAGAGAGCAAAGTCTCAAGATCATTACCTTGGAGACGCCCTACAAATGTTTTCCGTATCAGGGAAGATGTTCGCCCAATCTTCTGGGCTAATCGTCCTAGAAGCTACATATCAAGGACCATAGGTTGGGACCAGTATCCTCATGGTAGATGGAGTGATTCTAGTAATCCTTCATATGGGGCACTAACTGACTATCAGTTTATGCGGCCACGTGCACGTGACAAGAAGCTTCTTGAGGAATGGGCTCTCCCATTGAAAAGCATAGAGGATGTTTATGAGAAATTTATGAAGTACTGCCTTGGAAAGTTGAGAAGCAGTCCGTGGTCTGAATTAGACGGTCTTCAGGCTGAGACAAGAATAATAAGCGAACAGCTTGGTAAAATTAACATGAAAGGTTTCCTTTCAATCAACAGCCAACCAGCGGTTAATGGAGAAAAATCTGATTCTCCTTCTGTTGGATGGGGTGGATCAGGAGGTTACGTTTATCAAAAGGCATACATCGAGTTTTTCTGTTCCAGGGAAAACTTGGATGCTGTTGTTGACAAATGCAGGGGCCTTCCATCTCTAACCTACATGGCAGTGAATAAGGAAGGCATTTGGGTATCCAACATCACTCAAAATGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCGTCCAGCCAACTGTCGTCGATCCTGCCAGCTTTCTAGTTTGGAAAGATGAGGCATTTGAGATTTGGTCAAGGGGATGGGCTTCCTTGTACCCCGAGGGTGACCCGTCCCGAAAATTTCTCGAAGAGATACAGAGCAGCTATTACTTGGTGAGCTTGGTGGATAACAATTACGTCAATTCTAACCTCTTTGCTGCTTTGGAGGGATTCTGA

Protein sequence

MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGSTADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQDKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAYLKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCKTKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILMNLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSSNPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSRENLDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLVWKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF
Homology
[Close]
BLAST of Carg20746 vs. NCBI nr
Match: KAG7017155.1 (Methylenetetrahydrofolate reductase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1239.6 bits (3206), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM
Sbjct: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS
Sbjct: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
           AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN
Sbjct: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV
Sbjct: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 597
           WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF
Sbjct: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 596

[Close]
BLAST of Carg20746 vs. NCBI nr
Match: KAG6579712.1 (Methylenetetrahydrofolate reductase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1238.0 bits (3202), Expect = 0.0e+00
Identity = 595/596 (99.83%), Postives = 595/596 (99.83%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM
Sbjct: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS
Sbjct: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
           AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN
Sbjct: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV
Sbjct: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 597
           WKDEAFEIWSRGW SLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF
Sbjct: 541 WKDEAFEIWSRGWGSLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 596

[Close]
BLAST of Carg20746 vs. NCBI nr
Match: XP_022928798.1 (methylenetetrahydrofolate reductase 2-like [Cucurbita moschata])

HSP 1 Score: 1236.5 bits (3198), Expect = 0.0e+00
Identity = 595/596 (99.83%), Postives = 595/596 (99.83%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIR KYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM
Sbjct: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS
Sbjct: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
           AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN
Sbjct: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV
Sbjct: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 597
           WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF
Sbjct: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 596

[Close]
BLAST of Carg20746 vs. NCBI nr
Match: XP_023550795.1 (methylenetetrahydrofolate reductase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1226.5 bits (3172), Expect = 0.0e+00
Identity = 589/596 (98.83%), Postives = 593/596 (99.50%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIR KYGDYFGITVAGYPEAHPDVIGSNGVAT EGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM
Sbjct: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGW+QYPHGRWSDSS
Sbjct: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWAL LKS+EDVYEKFMKYCLGKLRSSPWSELDGLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
           AETRIISEQLGKINMKGFLSINSQPAVNGE+SDSPSVGWGGSGGYVYQKAYIEFFCSREN
Sbjct: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV
Sbjct: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 597
           WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDN+YVNSNLFAALEGF
Sbjct: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 596

[Close]
BLAST of Carg20746 vs. NCBI nr
Match: XP_022970234.1 (probable methylenetetrahydrofolate reductase [Cucurbita maxima])

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 589/596 (98.83%), Postives = 592/596 (99.33%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKIT ATAGDGGRVVFSFEYFPPKTEDGVDNLF+RMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIDKITEATAGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIR KYGDYFGITVAGYPEAHPDVIGSNGVAT EGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM
Sbjct: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS
Sbjct: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
           AETRIISEQLGKINMKGFLSINSQPAVNGE+SDSPSVGWGGSGGYVYQKAYIEFFCSREN
Sbjct: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV
Sbjct: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 597
           WKDEAFEIWSRGWASLYPEGD SRKFLEEIQSSYYLVSLVDN+YVNSNLFAALEGF
Sbjct: 541 WKDEAFEIWSRGWASLYPEGDLSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 596

[Close]
BLAST of Carg20746 vs. ExPASy Swiss-Prot
Match: O80585 (Methylenetetrahydrofolate reductase 2 OS=Arabidopsis thaliana OX=3702 GN=MTHFR2 PE=1 SV=2)

HSP 1 Score: 1028.1 bits (2657), Expect = 4.0e-299
Identity = 475/591 (80.37%), Postives = 536/591 (90.69%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKI   +  D G+  FSFE+FPPKTEDGVDNLF+RMDRMVA+ P+FCDITWGAGGS
Sbjct: 1   MKVIDKI--QSLADEGKTAFSFEFFPPKTEDGVDNLFERMDRMVAYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIA++MQN++CVE+MMHLTCTNMPVEKIDHAL+TI+SNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIASRMQNVVCVESMMHLTCTNMPVEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQ+EGGF CALDLV HIR KYGDYFGITVAGYPEAHPDVIG NG+A+ E YQSDL Y
Sbjct: 121 DKFVQVEGGFDCALDLVNHIRSKYGDYFGITVAGYPEAHPDVIGENGLASNEAYQSDLEY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKK DAGADLIVTQLFYDTDIFLKFVNDCRQIGI+CPIVPGIMPINNY+GFLRMT FCK
Sbjct: 181 LKKKIDAGADLIVTQLFYDTDIFLKFVNDCRQIGISCPIVPGIMPINNYRGFLRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP EVMAALEPIKDNEEAVKAYGIHLGTEMCKK+LAHG+K+LH+YTLNMEKSALAILM
Sbjct: 241 TKIPVEVMAALEPIKDNEEAVKAYGIHLGTEMCKKMLAHGVKSLHLYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLG+I+ESK+SRSLPWRRP NVFR +EDVRPIFWANRP+SYISRT GW+ +P GRW DS 
Sbjct: 301 NLGMIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWEDFPQGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           + SYGAL+D+QF RPRARDKKL +EW +PLKS+ED+ EKF + CLG L+SSPWSELDGLQ
Sbjct: 361 SASYGALSDHQFSRPRARDKKLQQEWVVPLKSVEDIQEKFKELCLGNLKSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ETRII+EQL K+N KGFL+INSQP+VN E+SDSP+VGWGG  GYVYQKAY+EFFCS+E 
Sbjct: 421 PETRIINEQLIKVNSKGFLTINSQPSVNAERSDSPTVGWGGPVGYVYQKAYLEFFCSKEK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDAVV+KC+ LPS+TYMAVNK   WVSN  Q DVNAVTWGVFPAKEI+QPT+VDPASF V
Sbjct: 481 LDAVVEKCKALPSITYMAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFA 592
           WKDEAFE WSR WA+LYPE DPSR  LEE+++SYYLVSLV+N+Y+N ++FA
Sbjct: 541 WKDEAFETWSRSWANLYPEADPSRNLLEEVKNSYYLVSLVENDYINGDIFA 589

[Close]
BLAST of Carg20746 vs. ExPASy Swiss-Prot
Match: Q75HE6 (Probable methylenetetrahydrofolate reductase OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0815200 PE=2 SV=1)

HSP 1 Score: 1027.7 bits (2656), Expect = 5.2e-299
Identity = 481/594 (80.98%), Postives = 532/594 (89.56%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVI+KI  A A   GR VFSFEYFPPKTE+G+DNLF+RMDRMVAH P+FCDITWGAGGS
Sbjct: 1   MKVIEKIQEAAA--DGRTVFSFEYFPPKTEEGLDNLFERMDRMVAHGPNFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IAN+MQNM+CVETMMHLTCTNMPVEKID AL TIKSNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANRMQNMVCVETMMHLTCTNMPVEKIDDALTTIKSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQ+ GGFACALDLV+HIR KYGDYFGITVAGYPEAHPD I S   AT E Y +DLAY
Sbjct: 121 DKFVQVAGGFACALDLVQHIRAKYGDYFGITVAGYPEAHPDAIQSTEGATPEAYSNDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LK+K DAGADLI+TQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCK
Sbjct: 181 LKQKVDAGADLIITQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP E+ AALEPIKDNEEAVKAYGIHLGTEMCKKILA GIKTLH+YTLNMEKSAL ILM
Sbjct: 241 TKIPAEITAALEPIKDNEEAVKAYGIHLGTEMCKKILATGIKTLHLYTLNMEKSALGILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLGLIEESK+SRSLPWR PTNVFR++EDVRPIFWANRP+SYISRT+GWDQYPHGRW DS 
Sbjct: 301 NLGLIEESKISRSLPWRPPTNVFRVKEDVRPIFWANRPKSYISRTLGWDQYPHGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQF RPR R KKL EEWA+P+KS+ED+ E+FM +C GKL SSPWSELDGLQ
Sbjct: 361 NPSYGALTDYQFTRPRGRGKKLQEEWAVPVKSVEDINERFMNFCQGKLTSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ET+II +QL KIN KGFL+INSQPAVNGE+SDS SVGWGG GGYVYQKAY+EFFCS+E 
Sbjct: 421 PETKIIDDQLVKINQKGFLTINSQPAVNGERSDSTSVGWGGPGGYVYQKAYLEFFCSKEK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LD +++K +  PSLTY+AVNK+G   SNI  N VNAVTWGVFP KEIVQPTVVD ASF+V
Sbjct: 481 LDQLIEKSKAFPSLTYIAVNKDGESFSNIPTNAVNAVTWGVFPGKEIVQPTVVDSASFMV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALE 595
           WKDEAFEIWS+GWA L+PEGD SR+ L+++Q SY+LVSLVDN+Y+N +LFAA +
Sbjct: 541 WKDEAFEIWSKGWACLFPEGDSSREILDKVQKSYFLVSLVDNDYINGDLFAAFK 592

[Close]
BLAST of Carg20746 vs. ExPASy Swiss-Prot
Match: Q9SE94 (Methylenetetrahydrofolate reductase 1 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 1019.2 bits (2634), Expect = 1.9e-296
Identity = 474/594 (79.80%), Postives = 530/594 (89.23%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVI+KI  A AGD GR  FSFEYFPPKTE+GV+NLF+RMDRMVAH PSFCDITWGAGGS
Sbjct: 1   MKVIEKILEA-AGD-GRTAFSFEYFPPKTEEGVENLFERMDRMVAHGPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IAN+MQNM+CVETMMHLTCTNMPVEKIDHAL+TIKSNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANRMQNMVCVETMMHLTCTNMPVEKIDHALETIKSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQ+EGGFACALDLV+HIR KYGDYFGITVAGYPEAHPD I   G AT E Y +DLAY
Sbjct: 121 DKFVQVEGGFACALDLVQHIRAKYGDYFGITVAGYPEAHPDAIQGEGGATLEAYSNDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LK+K DAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCK
Sbjct: 181 LKRKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP E+ AAL+PIKDNEEAV+ YGIHLGTEMCKKILA GIKTLH+YTLNM+KSA+ ILM
Sbjct: 241 TKIPSEITAALDPIKDNEEAVRQYGIHLGTEMCKKILATGIKTLHLYTLNMDKSAIGILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLGLIEESKVSR LPWR  TNVFR++EDVRPIFWANRP+SY+ RT+GWDQYPHGRW DS 
Sbjct: 301 NLGLIEESKVSRPLPWRPATNVFRVKEDVRPIFWANRPKSYLKRTLGWDQYPHGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTD+QF RPR R KKL EEWA+PLKS+ED+ E+F  +C GKL SSPWSELDGLQ
Sbjct: 361 NPSYGALTDHQFTRPRGRGKKLQEEWAVPLKSVEDISERFTNFCQGKLTSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ET+II +QL  IN KGFL+INSQPAVNGEKSDSP+VGWGG GGYVYQKAY+EFFC++E 
Sbjct: 421 PETKIIDDQLVNINQKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYLEFFCAKEK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LD +++K +  PSLTY+AVNK+G   SNI+ N VNAVTWGVFP KEI+QPTVVD ASF+V
Sbjct: 481 LDQLIEKIKAFPSLTYIAVNKDGETFSNISPNAVNAVTWGVFPGKEIIQPTVVDHASFMV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALE 595
           WKDEAFEIW+RGW  ++PEGD SR+ LE++Q +YYLVSLVDN+YV  +LFAA +
Sbjct: 541 WKDEAFEIWTRGWGCMFPEGDSSRELLEKVQKTYYLVSLVDNDYVQGDLFAAFK 592

[Close]
BLAST of Carg20746 vs. ExPASy Swiss-Prot
Match: Q9SE60 (Methylenetetrahydrofolate reductase 1 OS=Arabidopsis thaliana OX=3702 GN=MTHFR1 PE=1 SV=1)

HSP 1 Score: 1006.1 bits (2600), Expect = 1.6e-292
Identity = 458/591 (77.50%), Postives = 530/591 (89.68%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKV+DKI + T  + G+  FSFE+FPPKTEDGV+NLF+RMDR+V++ P+FCDITWGAGGS
Sbjct: 1   MKVVDKIKSVT--EQGQTAFSFEFFPPKTEDGVENLFERMDRLVSYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IA++MQN+ICVETMMHLTCTNMP+EKIDHAL+TI+SNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIASRMQNVICVETMMHLTCTNMPIEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQ+EGGFACALDLV HIR KYGDYFGITVAGYPEAHPDVI ++G+AT E YQSDLAY
Sbjct: 121 DKFVQVEGGFACALDLVNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKK DAGADLIVTQLFYDTDIFLKFVNDCRQIGI CPIVPGIMPI+NYKGFLRM  FCK
Sbjct: 181 LKKKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP E+ AALEPIKDN+EAVKAYGIH  TEMCKKILAHGI +LH+YTLN++KSA+ ILM
Sbjct: 241 TKIPAELTAALEPIKDNDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLGLI+ESK+SRSLPWRRP NVFR +EDVRPIFWANRP+SYISRT GW+ +PHGRW DS 
Sbjct: 301 NLGLIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSH 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           + +Y  L+DYQF RP+ RDKKL +EW +PLKSIEDV EKF + C+G L+SSPWSELDGLQ
Sbjct: 361 SAAYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQEKFKELCIGNLKSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ET+II+EQLGKIN  GFL+INSQP+VN  KSDSP++GWGG GGYVYQKAY+EFFCS++ 
Sbjct: 421 PETKIINEQLGKINSNGFLTINSQPSVNAAKSDSPAIGWGGPGGYVYQKAYLEFFCSKDK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LD +V+K +  PS+TYMAVNK   WVSN  ++DVNAVTWGVFPAKE++QPT+VDPASF V
Sbjct: 481 LDTLVEKSKAFPSITYMAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFA 592
           WKDEAFEIWSR WA+LYPE DPSRK LEE+++SYYLVSLVDNNY+N ++F+
Sbjct: 541 WKDEAFEIWSRSWANLYPEDDPSRKLLEEVKNSYYLVSLVDNNYINGDIFS 589

[Close]
BLAST of Carg20746 vs. ExPASy Swiss-Prot
Match: P42898 (Methylenetetrahydrofolate reductase OS=Homo sapiens OX=9606 GN=MTHFR PE=1 SV=3)

HSP 1 Score: 530.0 bits (1364), Expect = 3.4e-149
Identity = 262/590 (44.41%), Postives = 371/590 (62.88%), Query Frame = 0 

Query: 14  DGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGSTAD----LTLDIA 73
           + G   FS E+FPP+T +G  NL  R DRM A  P + D+TW   G         ++ IA
Sbjct: 54  ESGDKWFSLEFFPPRTAEGAVNLISRFDRMAAGGPLYIDVTWHPAGDPGSDKETSSMMIA 113

Query: 74  NKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQDKFVQIEGG 133
           +   N   +ET++H+TC    +E+I   L   K  GL+N++ALRGDP    D++ + EGG
Sbjct: 114 STAVNYCGLETILHMTCCRQRLEEITGHLHKAKQLGLKNIMALRGDPI--GDQWEEEEGG 173

Query: 134 FACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAYLKKKFDAGA 193
           F  A+DLVKHIR ++GDYF I VAGYP+ HP+         A  +++DL +LK+K  AGA
Sbjct: 174 FNYAVDLVKHIRSEFGDYFDICVAGYPKGHPE---------AGSFEADLKHLKEKVSAGA 233

Query: 194 DLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCKTKIPDEVMA 253
           D I+TQLF++ D F +FV  C  +GITCPIVPGI PI  Y    ++    K ++P E+  
Sbjct: 234 DFIITQLFFEADTFFRFVKACTDMGITCPIVPGIFPIQGYHSLRQLVKLSKLEVPQEIKD 293

Query: 254 ALEPIKDNEEAVKAYGIHLGTEMCKKILAHG-IKTLHMYTLNMEKSALAILMNLGLIEES 313
            +EPIKDN+ A++ YGI L   +C+++LA G +  LH YTLN E +   +L  LG+  E 
Sbjct: 294 VIEPIKDNDAAIRNYGIELAVSLCQELLASGLVPGLHFYTLNREMATTEVLKRLGMWTED 353

Query: 314 KVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSSNPSYGALT 373
              R LPW    +  R  EDVRPIFWA+RP+SYI RT  WD++P+GRW +SS+P++G L 
Sbjct: 354 P-RRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELK 413

Query: 374 DYQ--FMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLG-------KLRSSPWSELDGL 433
           DY   +++ ++  ++LL+ W   L S E V+E F+ Y  G       K+   PW++ + L
Sbjct: 414 DYYLFYLKSKSPKEELLKMWGEELTSEESVFEVFVLYLSGEPNRNGHKVTCLPWND-EPL 473

Query: 434 QAETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSRE 493
            AET ++ E+L ++N +G L+INSQP +NG+ S  P VGWG SGGYV+QKAY+EFF SRE
Sbjct: 474 AAETSLLKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRE 533

Query: 494 NLDAVVDKCRGLP-SLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASF 553
             +A++   +     + Y  VN +G  ++N  +   NAVTWG+FP +EI+QPTVVDP SF
Sbjct: 534 TAEALLQVLKKYELRVNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSF 593

Query: 554 LVWKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSN 589
           + WKDEAF +W   W  LY E  PSR  ++ I  +Y+LV+LVDN++   N
Sbjct: 594 MFWKDEAFALWIERWGKLYEEESPSRTIIQYIHDNYFLVNLVDNDFPLDN 630

[Close]
BLAST of Carg20746 vs. ExPASy TrEMBL
Match: A0A6J1EQ45 (Methylenetetrahydrofolate reductase OS=Cucurbita moschata OX=3662 GN=LOC111435611 PE=3 SV=1)

HSP 1 Score: 1236.5 bits (3198), Expect = 0.0e+00
Identity = 595/596 (99.83%), Postives = 595/596 (99.83%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIR KYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM
Sbjct: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS
Sbjct: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
           AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN
Sbjct: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV
Sbjct: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 597
           WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF
Sbjct: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 596

[Close]
BLAST of Carg20746 vs. ExPASy TrEMBL
Match: A0A6J1I4Y4 (Methylenetetrahydrofolate reductase OS=Cucurbita maxima OX=3661 GN=LOC111469179 PE=3 SV=1)

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 589/596 (98.83%), Postives = 592/596 (99.33%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKIT ATAGDGGRVVFSFEYFPPKTEDGVDNLF+RMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIDKITEATAGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIR KYGDYFGITVAGYPEAHPDVIGSNGVAT EGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM
Sbjct: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS
Sbjct: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
           AETRIISEQLGKINMKGFLSINSQPAVNGE+SDSPSVGWGGSGGYVYQKAYIEFFCSREN
Sbjct: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV
Sbjct: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 597
           WKDEAFEIWSRGWASLYPEGD SRKFLEEIQSSYYLVSLVDN+YVNSNLFAALEGF
Sbjct: 541 WKDEAFEIWSRGWASLYPEGDLSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 596

[Close]
BLAST of Carg20746 vs. ExPASy TrEMBL
Match: A0A5A7V2U8 (Methylenetetrahydrofolate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold767G00900 PE=3 SV=1)

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 566/596 (94.97%), Postives = 582/596 (97.65%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKI AAT GDGGRVVFSFEYFPPKTEDGVDNLF+RMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIK+NGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIR KYGDYFGITVAGYPEAHPDVIGSNGVAT EGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIPDE+MAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILM
Sbjct: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLGLIEESKVSRSLPWRRPTNVFR+REDVRPIFWANRPRSYISRTIGWDQYPHGRWSDS 
Sbjct: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSEL+GLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
           AETR+ISEQLGKINMKGFLSINSQPAVNGE+SDSPSVGWGG GGYVYQKAYIEFFCSRE 
Sbjct: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDA+VDKCR LPSLTYMAVNKEGIWVSN+TQ DVNAVTWGVFPAKEI+QPTVVDPASFLV
Sbjct: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 597
           WKDEAFEIWSRGWA+LYPEGD SRK L+EIQSSYYLVSLVDN+Y+NS+LFA LE F
Sbjct: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 596

[Close]
BLAST of Carg20746 vs. ExPASy TrEMBL
Match: A0A6J1DPD3 (Methylenetetrahydrofolate reductase OS=Momordica charantia OX=3673 GN=LOC111022964 PE=3 SV=1)

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 569/596 (95.47%), Postives = 583/596 (97.82%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVI+KI AATAGDGGRVVFSFEYFPPKTEDGVDNLF+RMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIEKIRAATAGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIR KYGDYFGITVAGYPEAHPDVIGSNGVAT EGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIPDE+MAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILM
Sbjct: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHVYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLGLIEESKV+RSLPWRRPTNVFRIREDVRPIFWANRP+SYISRTIGWDQYPHGRWSDSS
Sbjct: 301 NLGLIEESKVARSLPWRRPTNVFRIREDVRPIFWANRPKSYISRTIGWDQYPHGRWSDSS 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
           AETRIISEQLGKINMKGFLSINSQPAVNGE+SDSPSVGWGG GGYVYQKAY+EFFCSREN
Sbjct: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYLEFFCSREN 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDA+VDKCR LP+LTYMAVNKEGIWVSNITQ DVNAVTWGVFPAKEI+QPTVVDPASF V
Sbjct: 481 LDALVDKCRVLPNLTYMAVNKEGIWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 597
           WKDEAFEIW RGWASLYPEGD SRK LEE+QSSYYLVSLVDN+YV+SNLFA LE F
Sbjct: 541 WKDEAFEIWLRGWASLYPEGDLSRKLLEEVQSSYYLVSLVDNDYVHSNLFAILEDF 596

[Close]
BLAST of Carg20746 vs. ExPASy TrEMBL
Match: A0A0A0LIC6 (Methylenetetrahydrofolate reductase OS=Cucumis sativus OX=3659 GN=Csa_2G149470 PE=3 SV=1)

HSP 1 Score: 1187.9 bits (3072), Expect = 0.0e+00
Identity = 565/596 (94.80%), Postives = 580/596 (97.32%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKI AATAGDGGRVVFSFEYFPPKTEDGVDNLF+RMDRMVAHNPSFCDITWGAGGS
Sbjct: 43  MKVIDKIRAATAGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 102

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ
Sbjct: 103 TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 162

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIR KYGDYFGITVAGYPEAHPDVIGSNGVAT EGYQSDLAY
Sbjct: 163 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 222

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCK
Sbjct: 223 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 282

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIPDE+MAALEPIKDNEEAVKAYGIHLGTEMCKKIL HGIKTLH+YTLNMEKSALAILM
Sbjct: 283 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILTHGIKTLHIYTLNMEKSALAILM 342

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLGLIEESKVSRSLPWRRPTNVFR+REDVRPIFWANRPRSYISRTIGWDQYPHGRWSDS 
Sbjct: 343 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 402

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSEL+GLQ
Sbjct: 403 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 462

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
           AETR+ISEQLGKINMKGFLSINSQPAVNGE+SDSP VGWGG GGYVYQKAYIEFFCSRE 
Sbjct: 463 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPCVGWGGPGGYVYQKAYIEFFCSREK 522

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDA+VDKCR LPSLTYMAVNKEGIWVSN TQ DVNAVTWGVFPAKEI+QPTVVDPASF+V
Sbjct: 523 LDAIVDKCRALPSLTYMAVNKEGIWVSNATQKDVNAVTWGVFPAKEIIQPTVVDPASFIV 582

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 597
           WKDEAFEIWSRGWA+LYPEGD SRK L+EIQSSYYLVSLVDN+Y+NS+LFA LE F
Sbjct: 583 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 638

[Close]
BLAST of Carg20746 vs. TAIR 10
Match: AT2G44160.1 (methylenetetrahydrofolate reductase 2 )

HSP 1 Score: 1028.1 bits (2657), Expect = 2.8e-300
Identity = 475/591 (80.37%), Postives = 536/591 (90.69%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKI   +  D G+  FSFE+FPPKTEDGVDNLF+RMDRMVA+ P+FCDITWGAGGS
Sbjct: 1   MKVIDKI--QSLADEGKTAFSFEFFPPKTEDGVDNLFERMDRMVAYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIA++MQN++CVE+MMHLTCTNMPVEKIDHAL+TI+SNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIASRMQNVVCVESMMHLTCTNMPVEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQ+EGGF CALDLV HIR KYGDYFGITVAGYPEAHPDVIG NG+A+ E YQSDL Y
Sbjct: 121 DKFVQVEGGFDCALDLVNHIRSKYGDYFGITVAGYPEAHPDVIGENGLASNEAYQSDLEY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKK DAGADLIVTQLFYDTDIFLKFVNDCRQIGI+CPIVPGIMPINNY+GFLRMT FCK
Sbjct: 181 LKKKIDAGADLIVTQLFYDTDIFLKFVNDCRQIGISCPIVPGIMPINNYRGFLRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP EVMAALEPIKDNEEAVKAYGIHLGTEMCKK+LAHG+K+LH+YTLNMEKSALAILM
Sbjct: 241 TKIPVEVMAALEPIKDNEEAVKAYGIHLGTEMCKKMLAHGVKSLHLYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLG+I+ESK+SRSLPWRRP NVFR +EDVRPIFWANRP+SYISRT GW+ +P GRW DS 
Sbjct: 301 NLGMIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWEDFPQGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           + SYGAL+D+QF RPRARDKKL +EW +PLKS+ED+ EKF + CLG L+SSPWSELDGLQ
Sbjct: 361 SASYGALSDHQFSRPRARDKKLQQEWVVPLKSVEDIQEKFKELCLGNLKSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ETRII+EQL K+N KGFL+INSQP+VN E+SDSP+VGWGG  GYVYQKAY+EFFCS+E 
Sbjct: 421 PETRIINEQLIKVNSKGFLTINSQPSVNAERSDSPTVGWGGPVGYVYQKAYLEFFCSKEK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDAVV+KC+ LPS+TYMAVNK   WVSN  Q DVNAVTWGVFPAKEI+QPT+VDPASF V
Sbjct: 481 LDAVVEKCKALPSITYMAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFA 592
           WKDEAFE WSR WA+LYPE DPSR  LEE+++SYYLVSLV+N+Y+N ++FA
Sbjct: 541 WKDEAFETWSRSWANLYPEADPSRNLLEEVKNSYYLVSLVENDYINGDIFA 589

[Close]
BLAST of Carg20746 vs. TAIR 10
Match: AT3G59970.3 (methylenetetrahydrofolate reductase 1 )

HSP 1 Score: 1006.1 bits (2600), Expect = 1.2e-293
Identity = 458/591 (77.50%), Postives = 530/591 (89.68%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKV+DKI + T  + G+  FSFE+FPPKTEDGV+NLF+RMDR+V++ P+FCDITWGAGGS
Sbjct: 1   MKVVDKIKSVT--EQGQTAFSFEFFPPKTEDGVENLFERMDRLVSYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IA++MQN+ICVETMMHLTCTNMP+EKIDHAL+TI+SNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIASRMQNVICVETMMHLTCTNMPIEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQ+EGGFACALDLV HIR KYGDYFGITVAGYPEAHPDVI ++G+AT E YQSDLAY
Sbjct: 121 DKFVQVEGGFACALDLVNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKK DAGADLIVTQLFYDTDIFLKFVNDCRQIGI CPIVPGIMPI+NYKGFLRM  FCK
Sbjct: 181 LKKKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP E+ AALEPIKDN+EAVKAYGIH  TEMCKKILAHGI +LH+YTLN++KSA+ ILM
Sbjct: 241 TKIPAELTAALEPIKDNDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLGLI+ESK+SRSLPWRRP NVFR +EDVRPIFWANRP+SYISRT GW+ +PHGRW DS 
Sbjct: 301 NLGLIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSH 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           + +Y  L+DYQF RP+ RDKKL +EW +PLKSIEDV EKF + C+G L+SSPWSELDGLQ
Sbjct: 361 SAAYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQEKFKELCIGNLKSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ET+II+EQLGKIN  GFL+INSQP+VN  KSDSP++GWGG GGYVYQKAY+EFFCS++ 
Sbjct: 421 PETKIINEQLGKINSNGFLTINSQPSVNAAKSDSPAIGWGGPGGYVYQKAYLEFFCSKDK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LD +V+K +  PS+TYMAVNK   WVSN  ++DVNAVTWGVFPAKE++QPT+VDPASF V
Sbjct: 481 LDTLVEKSKAFPSITYMAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFA 592
           WKDEAFEIWSR WA+LYPE DPSRK LEE+++SYYLVSLVDNNY+N ++F+
Sbjct: 541 WKDEAFEIWSRSWANLYPEDDPSRKLLEEVKNSYYLVSLVDNNYINGDIFS 589

[Close]
BLAST of Carg20746 vs. TAIR 10
Match: AT3G59970.2 (methylenetetrahydrofolate reductase 1 )

HSP 1 Score: 692.6 bits (1786), Expect = 2.8e-199
Identity = 318/399 (79.70%), Postives = 362/399 (90.73%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKV+DKI + T  + G+  FSFE+FPPKTEDGV+NLF+RMDR+V++ P+FCDITWGAGGS
Sbjct: 1   MKVVDKIKSVT--EQGQTAFSFEFFPPKTEDGVENLFERMDRLVSYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IA++MQN+ICVETMMHLTCTNMP+EKIDHAL+TI+SNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIASRMQNVICVETMMHLTCTNMPIEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQ+EGGFACALDLV HIR KYGDYFGITVAGYPEAHPDVI ++G+AT E YQSDLAY
Sbjct: 121 DKFVQVEGGFACALDLVNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKK DAGADLIVTQLFYDTDIFLKFVNDCRQIGI CPIVPGIMPI+NYKGFLRM  FCK
Sbjct: 181 LKKKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP E+ AALEPIKDN+EAVKAYGIH  TEMCKKILAHGI +LH+YTLN++KSA+ ILM
Sbjct: 241 TKIPAELTAALEPIKDNDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLGLI+ESK+SRSLPWRRP NVFR +EDVRPIFWANRP+SYISRT GW+ +PHGRW DS 
Sbjct: 301 NLGLIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSH 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEK 400
           + +Y  L+DYQF RP+ RDKKL +EW +PLKSIEDV E+
Sbjct: 361 SAAYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQER 397

[Close]
BLAST of Carg20746 vs. TAIR 10
Match: AT3G59970.1 (methylenetetrahydrofolate reductase 1 )

HSP 1 Score: 692.2 bits (1785), Expect = 3.7e-199
Identity = 318/398 (79.90%), Postives = 361/398 (90.70%), Query Frame = 0 

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKV+DKI + T  + G+  FSFE+FPPKTEDGV+NLF+RMDR+V++ P+FCDITWGAGGS
Sbjct: 1   MKVVDKIKSVT--EQGQTAFSFEFFPPKTEDGVENLFERMDRLVSYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IA++MQN+ICVETMMHLTCTNMP+EKIDHAL+TI+SNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIASRMQNVICVETMMHLTCTNMPIEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180
           DKFVQ+EGGFACALDLV HIR KYGDYFGITVAGYPEAHPDVI ++G+AT E YQSDLAY
Sbjct: 121 DKFVQVEGGFACALDLVNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKK DAGADLIVTQLFYDTDIFLKFVNDCRQIGI CPIVPGIMPI+NYKGFLRM  FCK
Sbjct: 181 LKKKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP E+ AALEPIKDN+EAVKAYGIH  TEMCKKILAHGI +LH+YTLN++KSA+ ILM
Sbjct: 241 TKIPAELTAALEPIKDNDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360
           NLGLI+ESK+SRSLPWRRP NVFR +EDVRPIFWANRP+SYISRT GW+ +PHGRW DS 
Sbjct: 301 NLGLIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSH 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYE 399
           + +Y  L+DYQF RP+ RDKKL +EW +PLKSIEDV E
Sbjct: 361 SAAYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQE 396

The following BLAST results are available for this feature:
BLAST of Carg20746 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7017155.10.0e+00100.00Methylenetetrahydrofolate reductase 2, partial [Cucurbita argyrosperma subsp. ar... [more]
KAG6579712.10.0e+0099.83Methylenetetrahydrofolate reductase 2, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022928798.10.0e+0099.83methylenetetrahydrofolate reductase 2-like [Cucurbita moschata][more]
XP_023550795.10.0e+0098.83methylenetetrahydrofolate reductase 2-like [Cucurbita pepo subsp. pepo][more]
XP_022970234.10.0e+0098.83probable methylenetetrahydrofolate reductase [Cucurbita maxima][more]
BLAST of Carg20746 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O805854.0e-29980.37Methylenetetrahydrofolate reductase 2 OS=Arabidopsis thaliana OX=3702 GN=MTHFR2 ... [more]
Q75HE65.2e-29980.98Probable methylenetetrahydrofolate reductase OS=Oryza sativa subsp. japonica OX=... [more]
Q9SE941.9e-29679.80Methylenetetrahydrofolate reductase 1 OS=Zea mays OX=4577 PE=2 SV=1[more]
Q9SE601.6e-29277.50Methylenetetrahydrofolate reductase 1 OS=Arabidopsis thaliana OX=3702 GN=MTHFR1 ... [more]
P428983.4e-14944.41Methylenetetrahydrofolate reductase OS=Homo sapiens OX=9606 GN=MTHFR PE=1 SV=3[more]
BLAST of Carg20746 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1EQ450.0e+0099.83Methylenetetrahydrofolate reductase OS=Cucurbita moschata OX=3662 GN=LOC11143561... [more]
A0A6J1I4Y40.0e+0098.83Methylenetetrahydrofolate reductase OS=Cucurbita maxima OX=3661 GN=LOC111469179 ... [more]
A0A5A7V2U80.0e+0094.97Methylenetetrahydrofolate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A6J1DPD30.0e+0095.47Methylenetetrahydrofolate reductase OS=Momordica charantia OX=3673 GN=LOC1110229... [more]
A0A0A0LIC60.0e+0094.80Methylenetetrahydrofolate reductase OS=Cucumis sativus OX=3659 GN=Csa_2G149470 P... [more]
BLAST of Carg20746 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G44160.12.8e-30080.37methylenetetrahydrofolate reductase 2 [more]
AT3G59970.31.2e-29377.50methylenetetrahydrofolate reductase 1 [more]
AT3G59970.22.8e-19979.70methylenetetrahydrofolate reductase 1 [more]
AT3G59970.13.7e-19979.90methylenetetrahydrofolate reductase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003171Methylenetetrahydrofolate reductase-likePFAMPF02219MTHFRcoord: 15..303
e-value: 6.8E-123
score: 409.6
IPR003171Methylenetetrahydrofolate reductase-likeCDDcd00537MTHFRcoord: 20..302
e-value: 2.12942E-121
score: 358.081
NoneNo IPR availableGENE3D3.20.20.220coord: 1..306
e-value: 6.4E-119
score: 398.3
NoneNo IPR availablePANTHERPTHR45754METHYLENETETRAHYDROFOLATE REDUCTASEcoord: 1..592
NoneNo IPR availablePANTHERPTHR45754:SF4METHYLENETETRAHYDROFOLATE REDUCTASE 1coord: 1..592
IPR004621Eukaryotic-type methylenetetrahydrofolate reductaseTIGRFAMTIGR00677TIGR00677coord: 19..307
e-value: 3.0E-150
score: 497.1
IPR029041FAD-linked oxidoreductase-likeSUPERFAMILY51730FAD-linked oxidoreductasecoord: 14..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20746-RACarg20746-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009086 methionine biosynthetic process
biological_process GO:0035999 tetrahydrofolate interconversion
biological_process GO:0006555 methionine metabolic process
cellular_component GO:0005829 cytosol
molecular_function GO:0071949 FAD binding
molecular_function GO:0106312 methylenetetrahydrofolate reductase NADH activity
molecular_function GO:0106313 methylenetetrahydrofolate reductase NADPH activity
molecular_function GO:0004489 methylenetetrahydrofolate reductase (NAD(P)H) activity