Carg20367 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg20367
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	calmodulin-like protein 1
Location	Carg_Chr18: 11615476 .. 11616030 (-)
RNA-Seq Expression	Carg20367
Synteny	Carg20367

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGATGAACCCAAATCTCCTCGACCTGCAATACAGAGTCTCCAAGAAAAAGTTCCTCCGGGAGCCCTCCCGACTGTTCTCCTTCAAGGACCGGCAGAGCTCCGGCGTCCGGCGGGCTCTGCAACCGAGCACGGAGGAGATAAAGCAGATCTTCAACAAGTTCGACACGAACAGAGACGGGCGGATCTCAAAGCACGAATACAGGGGAGTTCTTAAGGCGCTGGGGAGAGGGAACTCGATGGAAGAGGTGCAGAAGATTTTCAGGGCGGTGGATACCGACGGCGATGGGTACATAAACTTGAATGAGTTCATGGAGGTTCACCGGAGTGGCGACGGAGTTCAGGCGAGAGATGTGGAATTTGCTTTTAAGACGTTTGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAATTATGAGAGTGTTGAGGGGATTAGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGTATGGTGGACATTGATGAGTTCATGACTATGATGACTCGCTCTGTGATGTAA

mRNA sequence

ATGCCGATGAACCCAAATCTCCTCGACCTGCAATACAGAGTCTCCAAGAAAAAGTTCCTCCGGGAGCCCTCCCGACTGTTCTCCTTCAAGGACCGGCAGAGCTCCGGCGTCCGGCGGGCTCTGCAACCGAGCACGGAGGAGATAAAGCAGATCTTCAACAAGTTCGACACGAACAGAGACGGGCGGATCTCAAAGCACGAATACAGGGGAGTTCTTAAGGCGCTGGGGAGAGGGAACTCGATGGAAGAGGTGCAGAAGATTTTCAGGGCGGTGGATACCGACGGCGATGGGTACATAAACTTGAATGAGTTCATGGAGGTTCACCGGAGTGGCGACGGAGTTCAGGCGAGAGATGTGGAATTTGCTTTTAAGACGTTTGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAATTATGAGAGTGTTGAGGGGATTAGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGTATGGTGGACATTGATGAGTTCATGACTATGATGACTCGCTCTGTGATGTAA

Coding sequence (CDS)

ATGCCGATGAACCCAAATCTCCTCGACCTGCAATACAGAGTCTCCAAGAAAAAGTTCCTCCGGGAGCCCTCCCGACTGTTCTCCTTCAAGGACCGGCAGAGCTCCGGCGTCCGGCGGGCTCTGCAACCGAGCACGGAGGAGATAAAGCAGATCTTCAACAAGTTCGACACGAACAGAGACGGGCGGATCTCAAAGCACGAATACAGGGGAGTTCTTAAGGCGCTGGGGAGAGGGAACTCGATGGAAGAGGTGCAGAAGATTTTCAGGGCGGTGGATACCGACGGCGATGGGTACATAAACTTGAATGAGTTCATGGAGGTTCACCGGAGTGGCGACGGAGTTCAGGCGAGAGATGTGGAATTTGCTTTTAAGACGTTTGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAATTATGAGAGTGTTGAGGGGATTAGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGTATGGTGGACATTGATGAGTTCATGACTATGATGACTCGCTCTGTGATGTAA

Protein sequence

MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSVM

Homology

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BLAST of Carg20367 vs. NCBI nr
Match: KAG6588656.1 (Calmodulin-like protein 30, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013282.1 Calmodulin-like protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 365.5 bits (937), Expect = 2.6e-97
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120
           GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE
Sbjct: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120

Query: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RSVM 185
           RSVM
Sbjct: 181 RSVM 184

[Close]

BLAST of Carg20367 vs. NCBI nr
Match: XP_022988967.1 (calmodulin-like protein 1 [Cucurbita maxima])

HSP 1 Score: 364.0 bits (933), Expect = 7.6e-97
Identity = 183/184 (99.46%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120
           GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQAR+VE
Sbjct: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQAREVE 120

Query: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RSVM 185
           RSVM
Sbjct: 181 RSVM 184

[Close]

BLAST of Carg20367 vs. NCBI nr
Match: XP_022927648.1 (calmodulin-like protein 1 [Cucurbita moschata])

HSP 1 Score: 362.8 bits (930), Expect = 1.7e-96
Identity = 183/184 (99.46%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120
           G ISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE
Sbjct: 61  GLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120

Query: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RSVM 185
           RSVM
Sbjct: 181 RSVM 184

[Close]

BLAST of Carg20367 vs. NCBI nr
Match: XP_023530136.1 (calmodulin-like protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 359.0 bits (920), Expect = 2.4e-95
Identity = 181/184 (98.37%), Postives = 182/184 (98.91%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120
           G ISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINL EFMEVH+SGDGVQARDVE
Sbjct: 61  GLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLKEFMEVHQSGDGVQARDVE 120

Query: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RSVM 185
           RSVM
Sbjct: 181 RSVM 184

[Close]

BLAST of Carg20367 vs. NCBI nr
Match: XP_004137114.2 (calmodulin-like protein 30 [Cucumis sativus] >KGN43936.1 hypothetical protein Csa_017094 [Cucumis sativus])

HSP 1 Score: 340.9 bits (873), Expect = 6.9e-90
Identity = 167/182 (91.76%), Postives = 179/182 (98.35%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN L+LQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS+EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120
           GRISKHEYRG+LKALGRGNSMEEVQKIFRAVD+DGDGYINLNEFMEVHRSG GVQA++VE
Sbjct: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

[Close]

BLAST of Carg20367 vs. ExPASy Swiss-Prot
Match: Q9ZQE6 (Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 2.5e-50
Identity = 97/182 (53.30%), Postives = 143/182 (78.57%), Query Frame = 0


Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRA-----LQPSTEEIKQIFNKFDTN 63
           N + L+LQY++SK K LR+PSR+FS +DRQSSG+         QPS  E++++F++FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  RDGRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARD 123
           +DG+IS+ EY+ VL+ALG+  ++E+V KIF+AVD DGDG+I+  EF++ ++   G+++ D
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSD 122

Query: 124 VEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTM 181
           +  +F TFDLNGD KISAEE+M VL  LGE+CS+EDC RMVRAVD+DGDG+V+++EF+ M
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKM 182

[Close]

BLAST of Carg20367 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 9.2e-29
Identity = 66/150 (44.00%), Postives = 97/150 (64.67%), Query Frame = 0


Query: 34  SSGVRRALQPSTEEIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNSMEEVQKIFRAVDT 93
           S  V R    S E+IK++F +FD N DG+IS  E + V+ AL    S EE + + +  D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 94  DGDGYINLNEFMEV----HRSGDGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEK 153
           DG+G+I+L+EF+ +     +S +    RD++ AF  +DL+ + +ISA E+  V++ LGEK
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 154 CSIEDCRRMVRAVDSDGDGMVDIDEFMTMM 180
           CSI+DC+RM+  VDSDGDG VD +EF  MM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of Carg20367 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 125.2 bits (313), Expect = 7.8e-28
Identity = 63/152 (41.45%), Postives = 101/152 (66.45%), Query Frame = 0


Query: 35  SGVRRALQPSTEEIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTD 94
           +GV R+   S ++IK++F +FD N DG+IS  E + V++AL    S EE   + +  D D
Sbjct: 5   NGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLD 64

Query: 95  GDGYINLNEFMEVHRSGDG------VQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGE 154
           G+G+I+L+EF+ + + G G          D++ AF+ +DL+G+ +ISA+E+  V++ LGE
Sbjct: 65  GNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGE 124

Query: 155 KCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 181
           KCS++DC++M+  VD DGDG V+ DEF  MM+
Sbjct: 125 KCSVQDCKKMISKVDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of Carg20367 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.3e-27
Identity = 63/152 (41.45%), Postives = 95/152 (62.50%), Query Frame = 0


Query: 31  DRQSSGVRRALQPSTEEIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNSMEEVQKIFRA 90
           D  S G   A  P  EE++++F KFD N DGRIS+ E   + ++LG   + +E+ ++   
Sbjct: 25  DPASGGGSPAPTPE-EEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAE 84

Query: 91  VDTDGDGYINLNEFMEVHRSGDGVQA---RDVEFAFKTFDLNGDRKISAEEIMRVLRGLG 150
            D DGDG+I+L+EF  ++ +  G  A    D+  AF+ FD +G+  ISA E+ RVL GLG
Sbjct: 85  ADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLG 144

Query: 151 EKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMM 180
           EK +++ CRRM+  VD +GDG++  +EF  MM
Sbjct: 145 EKATVQQCRRMIEGVDQNGDGLISFEEFKVMM 175

[Close]

BLAST of Carg20367 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.3e-27
Identity = 64/137 (46.72%), Postives = 90/137 (65.69%), Query Frame = 0


Query: 46  EEIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFM 105
           EE+K++F++FD+N DG+IS  E  GV KA+G   +  E+ ++   VDTD DGYINL+EF 
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 EVHRSGDGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSD 165
            + RS     A ++  AF  +D + +  ISA E+ +VL  LG  CS+EDC RM+  VD+D
Sbjct: 82  TLCRSSS--SAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 141

Query: 166 GDGMVDIDEFMTMMTRS 183
           GDG V+ +EF  MMT S
Sbjct: 142 GDGNVNFEEFQKMMTSS 156

[Close]

BLAST of Carg20367 vs. ExPASy TrEMBL
Match: A0A6J1JEH1 (calmodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111486169 PE=4 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 3.7e-97
Identity = 183/184 (99.46%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120
           GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQAR+VE
Sbjct: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQAREVE 120

Query: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RSVM 185
           RSVM
Sbjct: 181 RSVM 184

[Close]

BLAST of Carg20367 vs. ExPASy TrEMBL
Match: A0A6J1ELL0 (calmodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111434472 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 8.2e-97
Identity = 183/184 (99.46%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120
           G ISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE
Sbjct: 61  GLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120

Query: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RSVM 185
           RSVM
Sbjct: 181 RSVM 184

[Close]

BLAST of Carg20367 vs. ExPASy TrEMBL
Match: A0A0A0K804 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073720 PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 3.3e-90
Identity = 167/182 (91.76%), Postives = 179/182 (98.35%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN L+LQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS+EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120
           GRISKHEYRG+LKALGRGNSMEEVQKIFRAVD+DGDGYINLNEFMEVHRSG GVQA++VE
Sbjct: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

[Close]

BLAST of Carg20367 vs. ExPASy TrEMBL
Match: A0A5D3C643 (Calmodulin-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00450 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 7.4e-90
Identity = 167/182 (91.76%), Postives = 178/182 (97.80%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN LDLQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120
           GRISKHEYRG+LKALGRG+SMEEVQKIFRAVD+DGDGYINLNEFMEVHRSG GVQA++VE
Sbjct: 61  GRISKHEYRGILKALGRGSSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

[Close]

BLAST of Carg20367 vs. ExPASy TrEMBL
Match: A0A1S3C0X9 (calmodulin-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103495233 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 7.4e-90
Identity = 167/182 (91.76%), Postives = 178/182 (97.80%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN LDLQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120
           GRISKHEYRG+LKALGRG+SMEEVQKIFRAVD+DGDGYINLNEFMEVHRSG GVQA++VE
Sbjct: 61  GRISKHEYRGILKALGRGSSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

[Close]

BLAST of Carg20367 vs. TAIR 10
Match: AT2G15680.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 199.9 bits (507), Expect = 1.8e-51
Identity = 97/182 (53.30%), Postives = 143/182 (78.57%), Query Frame = 0


Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRA-----LQPSTEEIKQIFNKFDTN 63
           N + L+LQY++SK K LR+PSR+FS +DRQSSG+         QPS  E++++F++FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  RDGRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARD 123
           +DG+IS+ EY+ VL+ALG+  ++E+V KIF+AVD DGDG+I+  EF++ ++   G+++ D
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSD 122

Query: 124 VEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTM 181
           +  +F TFDLNGD KISAEE+M VL  LGE+CS+EDC RMVRAVD+DGDG+V+++EF+ M
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKM 182

[Close]

BLAST of Carg20367 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 128.3 bits (321), Expect = 6.6e-30
Identity = 66/150 (44.00%), Postives = 97/150 (64.67%), Query Frame = 0


Query: 34  SSGVRRALQPSTEEIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNSMEEVQKIFRAVDT 93
           S  V R    S E+IK++F +FD N DG+IS  E + V+ AL    S EE + + +  D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 94  DGDGYINLNEFMEV----HRSGDGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEK 153
           DG+G+I+L+EF+ +     +S +    RD++ AF  +DL+ + +ISA E+  V++ LGEK
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 154 CSIEDCRRMVRAVDSDGDGMVDIDEFMTMM 180
           CSI+DC+RM+  VDSDGDG VD +EF  MM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of Carg20367 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 125.2 bits (313), Expect = 5.5e-29
Identity = 63/152 (41.45%), Postives = 101/152 (66.45%), Query Frame = 0


Query: 35  SGVRRALQPSTEEIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTD 94
           +GV R+   S ++IK++F +FD N DG+IS  E + V++AL    S EE   + +  D D
Sbjct: 5   NGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLD 64

Query: 95  GDGYINLNEFMEVHRSGDG------VQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGE 154
           G+G+I+L+EF+ + + G G          D++ AF+ +DL+G+ +ISA+E+  V++ LGE
Sbjct: 65  GNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGE 124

Query: 155 KCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 181
           KCS++DC++M+  VD DGDG V+ DEF  MM+
Sbjct: 125 KCSVQDCKKMISKVDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of Carg20367 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 124.4 bits (311), Expect = 9.5e-29
Identity = 64/137 (46.72%), Postives = 90/137 (65.69%), Query Frame = 0


Query: 46  EEIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFM 105
           EE+K++F++FD+N DG+IS  E  GV KA+G   +  E+ ++   VDTD DGYINL+EF 
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 EVHRSGDGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSD 165
            + RS     A ++  AF  +D + +  ISA E+ +VL  LG  CS+EDC RM+  VD+D
Sbjct: 82  TLCRSSS--SAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 141

Query: 166 GDGMVDIDEFMTMMTRS 183
           GDG V+ +EF  MMT S
Sbjct: 142 GDGNVNFEEFQKMMTSS 156

[Close]

BLAST of Carg20367 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 124.4 bits (311), Expect = 9.5e-29
Identity = 64/137 (46.72%), Postives = 90/137 (65.69%), Query Frame = 0


Query: 46  EEIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFM 105
           EE+K++F++FD+N DG+IS  E  GV KA+G   +  E+ ++   VDTD DGYINL+EF 
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 EVHRSGDGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSD 165
            + RS     A ++  AF  +D + +  ISA E+ +VL  LG  CS+EDC RM+  VD+D
Sbjct: 82  TLCRSSS--SAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 141

Query: 166 GDGMVDIDEFMTMMTRS 183
           GDG V+ +EF  MMT S
Sbjct: 142 GDGNVNFEEFQKMMTSS 156

The following BLAST results are available for this feature:

BLAST of Carg20367 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6588656.1	2.6e-97	100.00	Calmodulin-like protein 30, partial [Cucurbita argyrosperma subsp. sororia] >KAG...	[more]
XP_022988967.1	7.6e-97	99.46	calmodulin-like protein 1 [Cucurbita maxima]	[more]
XP_022927648.1	1.7e-96	99.46	calmodulin-like protein 1 [Cucurbita moschata]	[more]
XP_023530136.1	2.4e-95	98.37	calmodulin-like protein 1 [Cucurbita pepo subsp. pepo]	[more]
XP_004137114.2	6.9e-90	91.76	calmodulin-like protein 30 [Cucumis sativus] >KGN43936.1 hypothetical protein Cs...	[more]

BLAST of Carg20367 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQE6	2.5e-50	53.30	Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1	[more]
Q9C8Y1	9.2e-29	44.00	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
P25070	7.8e-28	41.45	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]
Q8RZB5	1.3e-27	41.45	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9LE22	1.3e-27	46.72	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]

BLAST of Carg20367 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JEH1	3.7e-97	99.46	calmodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111486169 PE=4 SV=1	[more]
A0A6J1ELL0	8.2e-97	99.46	calmodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111434472 PE=4 SV=...	[more]
A0A0A0K804	3.3e-90	91.76	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073720 PE=4 SV=1	[more]
A0A5D3C643	7.4e-90	91.76	Calmodulin-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3C0X9	7.4e-90	91.76	calmodulin-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103495233 PE=4 SV=1	[more]

BLAST of Carg20367 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G15680.1	1.8e-51	53.30	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	6.6e-30	44.00	calmodulin like 23	[more]
AT5G37770.1	5.5e-29	41.45	EF hand calcium-binding protein family	[more]
AT1G18210.1	9.5e-29	46.72	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	9.5e-29	46.72	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 47..75 e-value: 1.2E-6 score: 38.1 coord: 83..111 e-value: 2.3E-5 score: 33.8 coord: 118..146 e-value: 0.0024 score: 27.1 coord: 154..182 e-value: 0.0063 score: 25.7
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 46..106 e-value: 2.4E-15 score: 56.7 coord: 119..179 e-value: 3.8E-12 score: 46.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 79..114 score: 14.178661
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 118..149 score: 12.477041
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 150..184 score: 11.193852
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 43..78 score: 15.629221
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 47..109 e-value: 1.23735E-18 score: 73.7361
None	No IPR available	GENE3D	1.10.238.10		coord: 113..183 e-value: 1.6E-20 score: 74.9
None	No IPR available	GENE3D	1.10.238.10		coord: 8..112 e-value: 3.5E-24 score: 87.7
None	No IPR available	PANTHER	PTHR10891:SF685	CALCIUM-BINDING PROTEIN CML34-RELATED	coord: 8..182
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 8..182
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 56..68
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 163..175
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 92..104
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 127..139
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 45..181

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg20367-RA	Carg20367-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005509	calcium ion binding